Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,075,234 C→A 100% D296Y (GAC→TAC)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,075,2340CA98.3% 576.8 / ‑0.4 192D296Y (GAC→TAC) fdoHformate dehydrogenase‑O, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (90/98);  total (94/98)
Fisher's exact test for biased strand distribution p-value = 5.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

CATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC  >  CP009273/4075089‑4075378
                                                                                                                                                 |                                                                                                                                                
cATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAtt                                                                                                                                                >  2:2519893/1‑148 (MQ=255)
cATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAtt                                                                                                                                                >  2:3541696/1‑148 (MQ=255)
 aTCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAttt                                                                                                                                               <  2:1230742/148‑1 (MQ=255)
  tCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtc                                                                                                                                              >  2:2212182/1‑148 (MQ=255)
    aaCCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtctc                                                                                                                                            <  1:1279954/148‑1 (MQ=255)
      ccAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCtt                                                                                                                                          >  2:623270/1‑148 (MQ=255)
        aGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCg                                                                                                                                        <  2:1087583/148‑1 (MQ=255)
        aGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCg                                                                                                                                        <  2:3516338/148‑1 (MQ=255)
              aGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGa                                                                                                                                  <  1:656383/148‑1 (MQ=255)
              aGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGa                                                                                                                                  <  2:2603456/148‑1 (MQ=255)
               ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGat                                                                                                                                 >  2:2791455/1‑148 (MQ=255)
                 aCGGTAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatta                                                                                                                               <  1:3099579/148‑1 (MQ=255)
                 aCGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatta                                                                                                                               <  2:28011/148‑1 (MQ=255)
                 aCGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatta                                                                                                                               >  2:2885933/1‑148 (MQ=255)
                   gggAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGattatt                                                                                                                             <  2:9192/148‑1 (MQ=255)
                     gAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           <  2:2947594/148‑1 (MQ=255)
                     gAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           >  2:2984611/1‑148 (MQ=255)
                      aaCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtctc                                                                                                                          >  2:720263/1‑148 (MQ=255)
                       aCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTctct                                                                                                                         >  1:1992723/1‑148 (MQ=255)
                       aCAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTctct                                                                                                                         >  2:1637267/1‑148 (MQ=255)
                        cAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCtt                                                                                                                        <  1:1925417/148‑1 (MQ=255)
                          aaaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTcc                                                                                                                      >  1:3137476/1‑148 (MQ=255)
                           aaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTCGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCt                                                                                                                     >  2:2753888/1‑148 (MQ=255)
                           aaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTGTCTTCGTGGAGATTATTCTCTTCCt                                                                                                                     >  1:985113/1‑148 (MQ=255)
                             agcccagcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCa                                                                                                                   <  1:1520094/148‑1 (MQ=255)
                              gcccagcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCAt                                                                                                                  <  1:1030100/148‑1 (MQ=255)
                               cccagcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATc                                                                                                                 <  2:2318944/148‑1 (MQ=255)
                                    cccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCAc                                                                                                            >  1:264280/1‑148 (MQ=255)
                                      cGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACgg                                                                                                          >  2:2273218/1‑148 (MQ=255)
                                      cGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACgg                                                                                                          >  1:397630/1‑148 (MQ=255)
                                        cTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGtt                                                                                                        <  2:3002523/148‑1 (MQ=255)
                                          cACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCGCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCg                                                                                                      >  1:2456001/1‑148 (MQ=255)
                                            ccgccgCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGa                                                                                                    <  1:25289/148‑1 (MQ=255)
                                             cgccgcCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGAc                                                                                                   >  2:1576351/1‑148 (MQ=255)
                                             cgccgcCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGAc                                                                                                   >  2:944144/1‑148 (MQ=255)
                                               ccgccAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCg                                                                                                 <  2:497049/148‑1 (MQ=255)
                                                  ccAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGaca                                                                                              >  2:2585220/1‑148 (MQ=255)
                                                   cAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGacac                                                                                             >  2:1284652/1‑148 (MQ=255)
                                                   cAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGacac                                                                                             >  2:3060042/1‑148 (MQ=255)
                                                     ggATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAccc                                                                                           >  2:1004837/1‑148 (MQ=255)
                                                      gATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTGATCGTATTTATCTTCGTGGAGATTATTATCATCCTCATCCGCACGGTTCGGACCGACACCCa                                                                                          >  1:260177/1‑148 (MQ=255)
                                                      gATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCa                                                                                          <  1:3416846/148‑1 (MQ=255)
                                                       aTGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCAc                                                                                         >  2:2302627/1‑148 (MQ=255)
                                                        tGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACg                                                                                        <  1:623270/148‑1 (MQ=255)
                                                         gAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGt                                                                                       <  2:2440686/148‑1 (MQ=255)
                                                          aaGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCCCGGTTCGGACCGACACCCACGTa                                                                                      <  1:768396/148‑1 (MQ=255)
                                                          aaGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTa                                                                                      <  1:3475408/148‑1 (MQ=255)
                                                          aaGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTa                                                                                      <  1:1117795/148‑1 (MQ=255)
                                                           aGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAg                                                                                     <  1:3239542/148‑1 (MQ=255)
                                                            gCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGt                                                                                    <  1:1314177/148‑1 (MQ=255)
                                                             cAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCGCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTg                                                                                   <  2:1381641/148‑1 (MQ=255)
                                                                  ggCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTATTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGa                                                                              >  2:1218213/1‑148 (MQ=255)
                                                                     ggTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATAc                                                                           <  1:3106494/148‑1 (MQ=255)
                                                                      gTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACt                                                                          >  2:1142835/1‑148 (MQ=255)
                                                                      gTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACCTAGTGGCAGATACt                                                                          >  1:3048468/1‑148 (MQ=255)
                                                                           ccAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCg                                                                     <  1:3228893/148‑1 (MQ=255)
                                                                             aGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCa                                                                   >  2:1592901/1‑148 (MQ=255)
                                                                             aGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCa                                                                   >  2:401646/1‑148 (MQ=255)
                                                                                ggTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAg                                                                >  1:521509/1‑148 (MQ=255)
                                                                                ggTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAg                                                                <  1:2885933/148‑1 (MQ=255)
                                                                                 gTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAgg                                                               <  1:1605501/148‑1 (MQ=255)
                                                                                 gTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAgg                                                               <  2:2149955/148‑1 (MQ=255)
                                                                                   tGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCGTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTa                                                             <  2:339655/148‑1 (MQ=255)
                                                                                    gATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAg                                                            <  1:3369768/148‑1 (MQ=255)
                                                                                     aTACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTCATTTGCGCTCCTCGTATTTCTCTTAGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTatc                                                           >  1:1910893/1‑146 (MQ=255)
                                                                                      tACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGcc                                                          >  2:491039/1‑148 (MQ=255)
                                                                                           tCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACCGGTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCaaa                                                     >  1:2847881/1‑148 (MQ=255)
                                                                                           tCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTCATTTGCGCGCCTCGTATTTCTCGTCGTGCAGATTATTATCTTCCTCCACCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCCAAGGTAGCCGCaaa                                                     >  1:1068584/1‑148 (MQ=255)
                                                                                             cGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGc                                                   <  1:328911/148‑1 (MQ=255)
                                                                                              ggCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGcc                                                  <  1:3055100/148‑1 (MQ=255)
                                                                                                cgcgGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTGCTGGGATTTCTCTTCGTGCAGATTTTTCTCTTGCTCATCCGCACGGTGCGGGCCGGCACCCCCGGAGTGGCAGAGACTGGGCGCAAAGGTAGCCGCAAAGccca                                                >  1:1009447/1‑146 (MQ=255)
                                                                                                cgcgGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGGAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGGAGCCGCAAAGCCaa                                                >  1:1009451/1‑148 (MQ=255)
                                                                                                    gtgtAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGc                                            <  1:843762/148‑1 (MQ=255)
                                                                                                         gcgcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGa                                       >  2:2613554/1‑148 (MQ=255)
                                                                                                          cgcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAg                                      >  2:2255418/1‑148 (MQ=255)
                                                                                                                aTGGTGTCACGTCGTTTCATTTGCGCACCTCGTAATTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGGAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGttt                                >  2:875641/1‑148 (MQ=255)
                                                                                                                  ggTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTcc                              <  2:1760779/148‑1 (MQ=255)
                                                                                                                    tgtCACGTCGTTTCATTTGGGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAg                            <  1:2116192/148‑1 (MQ=255)
                                                                                                                       cACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATg                         >  2:2664253/1‑148 (MQ=255)
                                                                                                                         cgtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGcc                       <  1:420584/148‑1 (MQ=255)
                                                                                                                          gtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCACAGCCAACAGCCGCGAGCGGTTTCCAGATGCCt                      >  2:509503/1‑148 (MQ=255)
                                                                                                                          gtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCt                      <  1:401646/148‑1 (MQ=255)
                                                                                                                          gtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCt                      <  2:1840945/148‑1 (MQ=255)
                                                                                                                            cgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCttt                    <  1:2465065/148‑1 (MQ=255)
                                                                                                                            cgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGGAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCttt                    <  2:384236/148‑1 (MQ=255)
                                                                                                                             gtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTc                   <  1:507182/148‑1 (MQ=255)
                                                                                                                              tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTcc                  >  2:2074924/1‑148 (MQ=255)
                                                                                                                              tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTcc                  >  2:2075004/1‑148 (MQ=255)
                                                                                                                              tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTcc                  >  2:3460762/1‑148 (MQ=255)
                                                                                                                                 cATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGa               <  1:3515849/148‑1 (MQ=255)
                                                                                                                                    ttGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAtt            >  2:862087/1‑148 (MQ=255)
                                                                                                                                    ttGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAtt            >  1:2448840/1‑148 (MQ=255)
                                                                                                                                    ttGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAtt            >  2:2716536/1‑148 (MQ=255)
                                                                                                                                         ctcctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACg       >  2:567802/1‑148 (MQ=255)
                                                                                                                                          tcctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAGAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACgg      <  1:2220959/148‑1 (MQ=255)
                                                                                                                                          tcctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAGAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACgg      <  1:2307444/148‑1 (MQ=255)
                                                                                                                                           cctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGt     <  1:731289/148‑1 (MQ=255)
                                                                                                                                             tcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCTCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGttt   <  2:397630/148‑1 (MQ=255)
                                                                                                                                             tcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGttt   >  2:320826/1‑148 (MQ=255)
                                                                                                                                             tcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGttt   <  1:2753888/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC  >  CP009273/4075089‑4075378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  CP009273/4075086‑4075383
                                                                                                                                                    |                                                                                                                                                     
TTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAT                                                                                                                                                      <  SRR19973066.2532717/150‑1 (MQ=60)
 TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATT                                                                                                                                                     <  SRR19973066.3439307/150‑1 (MQ=60)
 TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATT                                                                                                                                                     <  SRR19973066.3439283/150‑1 (MQ=60)
 TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATT                                                                                                                                                     <  SRR19973066.3439258/150‑1 (MQ=60)
       AACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTT                                                                                                                                               <  SRR19973066.1289201/150‑1 (MQ=60)
                AAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGAT                                                                                                                                      >  SRR19973066.125118/1‑150 (MQ=60)
                 AGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATT                                                                                                                                     <  SRR19973066.661172/150‑1 (MQ=60)
                 AGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATT                                                                                                                                     <  SRR19973066.661131/150‑1 (MQ=60)
                 AGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATT                                                                                                                                     >  SRR19973066.3544605/1‑150 (MQ=60)
                    ACGGTAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATT                                                                                                                                  <  SRR19973066.3120279/150‑1 (MQ=60)
                    ACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATT                                                                                                                                  <  SRR19973066.3120786/150‑1 (MQ=60)
                     CGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTC                                                                                                                                 <  SRR19973066.73009/150‑1 (MQ=60)
                      GGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCGCGTATTTCTCTTCGTGCAGATTATTCT                                                                                                                                >  SRR19973066.3075214/1‑150 (MQ=60)
                       GGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTC                                                                                                                               <  SRR19973066.2665662/150‑1 (MQ=60)
                        GAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCT                                                                                                                              >  SRR19973066.2006745/1‑150 (MQ=60)
                         AACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTT                                                                                                                             <  SRR19973066.3509234/150‑1 (MQ=60)
                          ACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTC                                                                                                                            <  SRR19973066.3160382/150‑1 (MQ=60)
                           CAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCC                                                                                                                           <  SRR19973066.1939109/150‑1 (MQ=60)
                           CAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCC                                                                                                                           >  SRR19973066.3158414/1‑150 (MQ=60)
                            AAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTGTCTTCGTGGAGATTATTCTCTTCCT                                                                                                                          >  SRR19973066.992380/1‑150 (MQ=60)
                            AAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCT                                                                                                                          <  SRR19973066.2930557/150‑1 (MQ=60)
                            AAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTCATTTGCGCTCCTCGTCTTTCTCTTCGTGGAGATTATTCTCTTCCT                                                                                                                          >  SRR19973066.992382/1‑150 (MQ=60)
                                AGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATC                                                                                                                      <  SRR19973066.1530812/150‑1 (MQ=60)
                                 GCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCC                                                                                                                     >  SRR19973066.2028804/1‑150 (MQ=60)
                                 GCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCC                                                                                                                     <  SRR19973066.1037593/150‑1 (MQ=60)
                                 GCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCC                                                                                                                     <  SRR19973066.1037689/150‑1 (MQ=60)
                                   CCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGC                                                                                                                   <  SRR19973066.1071918/150‑1 (MQ=60)
                                   CCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGC                                                                                                                   <  SRR19973066.3158345/150‑1 (MQ=60)
                                     AGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTACTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCAACCGCAC                                                                                                                 >  SRR19973066.193123/1‑150 (MQ=60)
                                     AGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCAC                                                                                                                 >  SRR19973066.266260/1‑150 (MQ=60)
                                      GCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACG                                                                                                                <  SRR19973066.2317401/150‑1 (MQ=60)
                                       CCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTATCTTCCTCATCCGCACGG                                                                                                               >  SRR19973066.400760/1‑150 (MQ=60)
                                       CCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTATCTTCCTCAACCGCACGG                                                                                                               >  SRR19973066.400761/1‑150 (MQ=60)
                                         CGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATACGCACGGTT                                                                                                             >  SRR19973066.347067/1‑150 (MQ=60)
                                           CTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCGCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCG                                                                                                           >  SRR19973066.2472738/1‑150 (MQ=60)
                                           CTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCGCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCG                                                                                                           >  SRR19973066.2472735/1‑150 (MQ=60)
                                               CCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACC                                                                                                       <  SRR19973066.25650/150‑1 (MQ=60)
                                                       AGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTGATCGTATTTATCTTCGTGGAGATTATTATCATCCTCATCCGCACGGTTCGGACCGACACCCA                                                                                               >  SRR19973066.262134/1‑150 (MQ=60)
                                                         GATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACG                                                                                             <  SRR19973066.3439695/150‑1 (MQ=60)
                                                           TGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTA                                                                                           <  SRR19973066.627713/150‑1 (MQ=60)
                                                             AAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCCCGGTTCGGACCGACACCCACGTAGT                                                                                         <  SRR19973066.774089/150‑1 (MQ=60)
                                                             AAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGT                                                                                         <  SRR19973066.3498612/150‑1 (MQ=60)
                                                             AAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGT                                                                                         <  SRR19973066.1125855/150‑1 (MQ=60)
                                                              AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTG                                                                                        <  SRR19973066.3260920/150‑1 (MQ=60)
                                                              AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTGATTTGCGCGCCGCGTATTTCTCTTCGTGCCGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGGAGTG                                                                                        >  SRR19973066.3308748/1‑150 (MQ=60)
                                                               GCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGG                                                                                       <  SRR19973066.1323709/150‑1 (MQ=60)
                                                               GCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGG                                                                                       <  SRR19973066.1578859/150‑1 (MQ=60)
                                                                       CGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACCTAGTGGCAGATACT                                                                               >  SRR19973066.3068846/1‑150 (MQ=60)
                                                                        GGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTG                                                                              <  SRR19973066.3127239/150‑1 (MQ=60)
                                                                           GATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCC                                                                           <  SRR19973066.1294070/150‑1 (MQ=60)
                                                                              CCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCA                                                                        <  SRR19973066.3250229/150‑1 (MQ=60)
                                                                              ACAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCA                                                                        <  SRR19973066.3250806/150‑1 (MQ=60)
                                                                                 GTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAG                                                                     >  SRR19973066.525343/1‑150 (MQ=60)
                                                                                 GTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAG                                                                     >  SRR19973066.524466/1‑150 (MQ=60)
                                                                                   GGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGT                                                                   <  SRR19973066.2905248/150‑1 (MQ=60)
                                                                                   GGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGT                                                                   <  SRR19973066.2435508/150‑1 (MQ=60)
                                                                                    GTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTA                                                                  <  SRR19973066.1617151/150‑1 (MQ=60)
                                                                                    GTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTA                                                                  <  SRR19973066.803790/150‑1 (MQ=60)
                                                                                    GTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTA                                                                  <  SRR19973066.1112420/150‑1 (MQ=60)
                                                                                      TGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTCATTTGCGCTCCTCGTATTTCTCTTAGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTATC                                                                >  SRR19973066.1924500/1‑150 (MQ=60)
                                                                                       GATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCC                                                               <  SRR19973066.3392245/150‑1 (MQ=60)
                                                                                        ATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTTCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGCCCGACACCCACGTAGTGGGAGATACTGGCCGCCAAGGTAGCCG                                                              >  SRR19973066.1522522/1‑150 (MQ=60)
                                                                                         TACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGC                                                             >  SRR19973066.3340954/1‑150 (MQ=60)
                                                                                            GTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACCGGTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAA                                                          >  SRR19973066.2867015/1‑150 (MQ=60)
                                                                                            GTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTGTCATTTGCGCGCCTCGTATTTCTCGTCGTGCAGATTATTATCTTCCTCCACCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCCAAGGTAGCCGCAAA                                                          >  SRR19973066.1076416/1‑150 (MQ=60)
                                                                                                CGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCA                                                      <  SRR19973066.331417/150‑1 (MQ=60)
                                                                                                 GGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTGCTGGGATTTCTCTTCGTGCAGATTTTTCTCTTGCTCATCCGCACGGTGCGGGCCGGCACCCCCGGAGTGGCAGAGACTGGGCGCAAAGGTAGCCGCAAAGCCCA                                                     >  SRR19973066.1016882/1‑150 (MQ=60)
                                                                                                 GGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGGAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGGAGCCGCAAAGCCAA                                                     >  SRR19973066.1016886/1‑150 (MQ=60)
                                                                                                 GGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCCAAGCCAA                                                     >  SRR19973066.2878588/1‑150 (MQ=60)
                                                                                                 GGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAA                                                     <  SRR19973066.3075533/150‑1 (MQ=60)
                                                                                                 GGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAA                                                     >  SRR19973066.2811570/1‑150 (MQ=60)
                                                                                                       GTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCG                                               <  SRR19973066.849890/150‑1 (MQ=60)
                                                                                                        TGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCGCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGGTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCCAAGGTAGCCGCAAAGCCAACAGCCGC                                              >  SRR19973066.265418/1‑150 (MQ=60)
                                                                                                           AGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAG                                           <  SRR19973066.762369/150‑1 (MQ=60)
                                                                                                                       TGTCACGTCGTTTCATTTGGGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGAT                               <  SRR19973066.2130816/150‑1 (MQ=60)
                                                                                                                       TGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGAT                               <  SRR19973066.172310/150‑1 (MQ=60)
                                                                                                                            CGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTT                          <  SRR19973066.423858/150‑1 (MQ=60)
                                                                                                                             GTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTT                         <  SRR19973066.2602740/150‑1 (MQ=60)
                                                                                                                             GTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTT                         <  SRR19973066.404809/150‑1 (MQ=60)
                                                                                                                               CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                       <  SRR19973066.2481872/150‑1 (MQ=60)
                                                                                                                               CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                       <  SRR19973066.1980023/150‑1 (MQ=60)
                                                                                                                               CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                       <  SRR19973066.2481871/150‑1 (MQ=60)
                                                                                                                                GTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCA                      <  SRR19973066.510946/150‑1 (MQ=60)
                                                                                                                                    CATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAT                  <  SRR19973066.3539262/150‑1 (MQ=60)
                                                                                                                                     ATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATT                 >  SRR19973066.2465525/1‑150 (MQ=60)
                                                                                                                                      TTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTT                <  SRR19973066.951032/150‑1 (MQ=60)
                                                                                                                                       TTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTC               <  SRR19973066.451312/150‑1 (MQ=60)
                                                                                                                                         GCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCAC             <  SRR19973066.342864/150‑1 (MQ=60)
                                                                                                                                             TCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAGAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTT         <  SRR19973066.2323246/150‑1 (MQ=60)
                                                                                                                                             TCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAAGAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTT         <  SRR19973066.2236322/150‑1 (MQ=60)
                                                                                                                                              CCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT        <  SRR19973066.736711/150‑1 (MQ=60)
                                                                                                                                              CCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT        <  SRR19973066.2537030/150‑1 (MQ=60)
                                                                                                                                               CTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC       <  SRR19973066.538901/150‑1 (MQ=60)
                                                                                                                                                TCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCG      <  SRR19973066.2772431/150‑1 (MQ=60)
                                                                                                                                                  GTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCT    <  SRR19973066.2227501/150‑1 (MQ=60)
                                                                                                                                                   TATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTG   <  SRR19973066.3160744/150‑1 (MQ=60)
                                                                                                                                                   TATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTG   >  SRR19973066.1891379/1‑150 (MQ=60)
                                                                                                                                                    ATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  <  SRR19973066.131517/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  CP009273/4075086‑4075383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑