Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,075,234 C→A D296Y (GAC→TAC)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,075,2340CA100.0% 702.5 / NA 211D296Y (GAC→TAC) fdoHformate dehydrogenase‑O, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (88/123);  total (88/123)

Only 100 of 211 total aligned reads displayed.

TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC  >  CP009273/4075087‑4075378
                                                                                                                                                   |                                                                                                                                                
tGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAtt                                                                                                                                                <  1:2689076/150‑1 (MQ=255)
tGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAtt                                                                                                                                                <  2:673143/150‑1 (MQ=255)
tGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAtt                                                                                                                                                <  1:368070/150‑1 (MQ=255)
 gCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTAttt                                                                                                                                               <  2:1414482/150‑1 (MQ=255)
   aTCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             <  1:1042593/150‑1 (MQ=255)
   aTCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             >  1:3213635/1‑150 (MQ=255)
   aTCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             <  2:2001361/150‑1 (MQ=255)
   aTCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             >  1:654577/1‑150 (MQ=255)
   aTCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtct                                                                                                                                             <  1:3417936/150‑1 (MQ=255)
    tCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTtctc                                                                                                                                            >  2:1011544/1‑150 (MQ=255)
       aCCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTc                                                                                                                                         >  2:346120/1‑150 (MQ=255)
        ccAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCg                                                                                                                                        <  1:573078/150‑1 (MQ=255)
          aGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTg                                                                                                                                      >  2:1953854/1‑150 (MQ=255)
             tGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAg                                                                                                                                   >  1:2265713/1‑150 (MQ=255)
              gAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGa                                                                                                                                  >  2:1085970/1‑150 (MQ=255)
               aaGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGat                                                                                                                                 >  2:3476301/1‑150 (MQ=255)
                aGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatt                                                                                                                                >  2:607980/1‑150 (MQ=255)
                 ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatta                                                                                                                               <  2:2274863/150‑1 (MQ=255)
                 ggACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGatta                                                                                                                               <  2:2662518/150‑1 (MQ=255)
                    cGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtc                                                                                                                            <  1:1969489/150‑1 (MQ=255)
                    cGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtc                                                                                                                            <  1:217065/150‑1 (MQ=255)
                     gggAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           <  2:958804/150‑1 (MQ=255)
                     gggAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           >  2:2832864/1‑150 (MQ=255)
                     gggAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           <  1:2006872/150‑1 (MQ=255)
                     gggAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATtct                                                                                                                           >  2:293123/1‑150 (MQ=255)
                     gggAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGGATTTCTCTTCGTGCAGATTATtct                                                                                                                           >  2:2516990/1‑150 (MQ=255)
                       gAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTctct                                                                                                                         <  1:3461848/150‑1 (MQ=255)
                            aaaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTc                                                                                                                    <  1:2524773/150‑1 (MQ=255)
                             aaaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCa                                                                                                                   >  2:3639927/1‑150 (MQ=255)
                              aaGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCAt                                                                                                                  >  1:3364236/1‑150 (MQ=255)
                                  ccagcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGc                                                                                                              >  1:1567146/1‑150 (MQ=255)
                                   cagcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCa                                                                                                             >  1:1546507/1‑150 (MQ=255)
                                     gcccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACg                                                                                                           <  2:2819777/150‑1 (MQ=255)
                                      cccGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACgg                                                                                                          >  1:1331609/1‑150 (MQ=255)
                                        cGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGtt                                                                                                        <  1:3661076/150‑1 (MQ=255)
                                        cGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCCCATCCGCACGGtt                                                                                                        <  1:2787174/150‑1 (MQ=255)
                                         gCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTc                                                                                                       <  2:1491073/150‑1 (MQ=255)
                                         gCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTc                                                                                                       <  2:1635816/150‑1 (MQ=255)
                                          cTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCg                                                                                                      <  1:991459/150‑1 (MQ=255)
                                          cTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCg                                                                                                      <  2:1518367/150‑1 (MQ=255)
                                           tCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTctct                                                                                                                         >  1:2999052/1‑130 (MQ=255)
                                           tCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCgg                                                                                                     >  1:2666872/1‑150 (MQ=255)
                                            cACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGa                                                                                                    >  2:2793904/1‑150 (MQ=255)
                                              ccgccgCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGAcc                                                                                                  <  1:617561/150‑1 (MQ=255)
                                              ccgccgCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGAcc                                                                                                  <  1:1233134/150‑1 (MQ=255)
                                               cgccgcCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCg                                                                                                 >  1:2376444/1‑150 (MQ=255)
                                                gccgccAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGGATTTCTCTTCGTGCAGATTATTCTCTTCCTCCTCCGCACGGTTCGGACCGa                                                                                                <  2:2062246/150‑1 (MQ=255)
                                                            aaGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGt                                                                                    <  2:1962141/150‑1 (MQ=255)
                                                             aGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTg                                                                                   <  1:2404579/150‑1 (MQ=255)
                                                             aGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTg                                                                                   <  1:2629014/150‑1 (MQ=255)
                                                             aGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTg                                                                                   <  1:1160660/150‑1 (MQ=255)
                                                               cAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGa                                                                                 >  2:3078662/1‑150 (MQ=255)
                                                                aGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGaa                                                                                >  1:1025674/1‑150 (MQ=255)
                                                                aGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGaa                                                                                >  1:2927947/1‑150 (MQ=255)
                                                                 gAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAg                                                                               <  1:936684/150‑1 (MQ=255)
                                                                 gAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAg                                                                               <  2:715385/150‑1 (MQ=255)
                                                                   aGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAt                                                                             <  2:831693/150‑1 (MQ=255)
                                                                   aGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAt                                                                             <  1:2653453/150‑1 (MQ=255)
                                                                      cGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACt                                                                          >  2:1139807/1‑150 (MQ=255)
                                                                          gATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGcc                                                                      <  1:1007439/150‑1 (MQ=255)
                                                                          gATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGcc                                                                      <  2:3434672/150‑1 (MQ=255)
                                                                              cAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCaa                                                                  >  2:1886784/1‑150 (MQ=255)
                                                                                   gTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTa                                                             >  1:3644836/1‑150 (MQ=255)
                                                                                    ttGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAg                                                            <  2:2778363/150‑1 (MQ=255)
                                                                                       aTACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCg                                                         >  2:1405716/1‑150 (MQ=255)
                                                                                       aTACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCg                                                         <  2:1568506/150‑1 (MQ=255)
                                                                                         aCGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCa                                                       <  2:47457/150‑1 (MQ=255)
                                                                                          cGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCaa                                                      >  2:794453/1‑150 (MQ=255)
                                                                                           gTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCaaa                                                     <  1:1954591/150‑1 (MQ=255)
                                                                                            ttCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAg                                                    >  2:2729386/1‑150 (MQ=255)
                                                                                               cGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAATCCa                                                 >  1:1914922/1‑150 (MQ=255)
                                                                                                  cgcgGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACa                                              >  1:457214/1‑150 (MQ=255)
                                                                                                  cgcgGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACa                                              >  1:2353470/1‑150 (MQ=255)
                                                                                                     ggTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGcc                                           <  2:1694932/150‑1 (MQ=255)
                                                                                                     ggTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGcc                                           <  1:335379/150‑1 (MQ=255)
                                                                                                        gtAGCTCACTATTGTGTCACGTCGTTTCATTTGCGCTACTCTTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCctcg                                        <  2:3267700/150‑1 (MQ=255)
                                                                                                         tAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGa                                       >  1:2442834/1‑150 (MQ=255)
                                                                                                           gcgcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGc                                     <  1:3611502/150‑1 (MQ=255)
                                                                                                           gcgcACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGc                                     <  2:1865492/150‑1 (MQ=255)
                                                                                                                  aTGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTcc                              <  2:1487004/150‑1 (MQ=255)
                                                                                                                  aTGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTcc                              <  2:3083238/150‑1 (MQ=255)
                                                                                                                   tGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCa                             <  2:369299/150‑1 (MQ=255)
                                                                                                                     gtgtCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGa                           <  1:3386304/150‑1 (MQ=255)
                                                                                                                      tgtCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGAt                          <  2:634337/150‑1 (MQ=255)
                                                                                                                       gtCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATg                         <  1:2475954/150‑1 (MQ=255)
                                                                                                                          aCGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCt                      <  1:1139807/150‑1 (MQ=255)
                                                                                                                            gtcgtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGACGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCttt                    >  2:3532636/1‑150 (MQ=255)
                                                                                                                               gtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCa                 >  1:3394023/1‑150 (MQ=255)
                                                                                                                               gtTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCa                 >  2:1776648/1‑150 (MQ=255)
                                                                                                                                tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAg                >  1:3582697/1‑150 (MQ=255)
                                                                                                                                tttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAg                >  1:782994/1‑150 (MQ=255)
                                                                                                                                 ttCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGa               >  2:629696/1‑150 (MQ=255)
                                                                                                                                  tCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGaa              <  2:1567146/150‑1 (MQ=255)
                                                                                                                                  tCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGaa              >  1:3710660/1‑150 (MQ=255)
                                                                                                                                   cATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAt             <  1:2965894/150‑1 (MQ=255)
                                                                                                                                    aTTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAtt            >  1:1054271/1‑150 (MQ=255)
                                                                                                                                        gcgcTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCAc        <  1:1244942/150‑1 (MQ=255)
                                                                                                                                           ctcctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGt     <  2:2449262/150‑1 (MQ=255)
                                                                                                                                            tcctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGtt    <  2:2158329/150‑1 (MQ=255)
                                                                                                                                             cctcGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGACGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGttt   >  1:1300672/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTC  >  CP009273/4075087‑4075378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  CP009273/4075085‑4075383
                                                                                                                                                     |                                                                                                                                                     
TTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTA                                                                                                                                                       <  SRR19973061.113794/150‑1 (MQ=60)
  TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATT                                                                                                                                                     <  SRR19973061.2695925/150‑1 (MQ=60)
  TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATT                                                                                                                                                     <  SRR19973061.369070/150‑1 (MQ=60)
  TGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATT                                                                                                                                                     <  SRR19973061.2695991/150‑1 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  <  SRR19973061.1044870/150‑1 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  <  SRR19973061.1045260/150‑1 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  >  SRR19973061.3221890/1‑150 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  >  SRR19973061.656261/1‑150 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  <  SRR19973061.3426528/150‑1 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  <  SRR19973061.3426547/150‑1 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  <  SRR19973061.3426708/150‑1 (MQ=60)
     ATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCT                                                                                                                                                  >  SRR19973061.2979915/1‑150 (MQ=60)
      TCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTC                                                                                                                                                 <  SRR19973061.1181546/150‑1 (MQ=60)
         ACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTC                                                                                                                                              <  SRR19973061.2405445/150‑1 (MQ=60)
          CCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCG                                                                                                                                             <  SRR19973061.574584/150‑1 (MQ=60)
          CCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCG                                                                                                                                             <  SRR19973061.574572/150‑1 (MQ=60)
            AGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTG                                                                                                                                           <  SRR19973061.1146863/150‑1 (MQ=60)
              TTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCA                                                                                                                                         <  SRR19973061.337014/150‑1 (MQ=60)
               TGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAG                                                                                                                                        >  SRR19973061.2271428/1‑150 (MQ=60)
                    GACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTAT                                                                                                                                   <  SRR19973061.2369423/150‑1 (MQ=60)
                      CGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTC                                                                                                                                 <  SRR19973061.217703/150‑1 (MQ=60)
                      CGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTC                                                                                                                                 <  SRR19973061.1974417/150‑1 (MQ=60)
                      CGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTC                                                                                                                                 <  SRR19973061.631247/150‑1 (MQ=60)
                       GGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCT                                                                                                                                <  SRR19973061.2011907/150‑1 (MQ=60)
                       GGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCT                                                                                                                                <  SRR19973061.2011463/150‑1 (MQ=60)
                       GGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCT                                                                                                                                <  SRR19973061.3347781/150‑1 (MQ=60)
                         GAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCT                                                                                                                              <  SRR19973061.3470721/150‑1 (MQ=60)
                          AACAAAAAGCCCAGCCCGCTCACCGTCGCTATGATGAAGCAGAAGGAGGTGATTCAGTGGTTGATATGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTT                                                                                                                             <  SRR19973061.2950614/150‑1 (MQ=60)
                              AAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTC                                                                                                                         <  SRR19973061.2531201/150‑1 (MQ=60)
                                AAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCAT                                                                                                                       >  SRR19973061.3372885/1‑150 (MQ=60)
                                    CCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGC                                                                                                                   >  SRR19973061.1571039/1‑150 (MQ=60)
                                     CAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCA                                                                                                                  >  SRR19973061.1550353/1‑150 (MQ=60)
                                      AGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCAC                                                                                                                 >  SRR19973061.2455506/1‑150 (MQ=60)
                                        CCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGG                                                                                                               >  SRR19973061.1334953/1‑150 (MQ=60)
                                         CCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGT                                                                                                              <  SRR19973061.765195/150‑1 (MQ=60)
                                          CGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTT                                                                                                             <  SRR19973061.3670425/150‑1 (MQ=60)
                                          CGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTT                                                                                                             <  SRR19973061.3670435/150‑1 (MQ=60)
                                          CGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCCCATCCGCACGGTT                                                                                                             <  SRR19973061.2794342/150‑1 (MQ=60)
                                           GCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTC                                                                                                            <  SRR19973061.1708040/150‑1 (MQ=60)
                                            CTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCG                                                                                                           <  SRR19973061.993991/150‑1 (MQ=60)
                                            CTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCG                                                                                                           <  SRR19973061.830117/150‑1 (MQ=60)
                                             TCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTagatcggaagagcacacgtc                                                                                                          >  SRR19973061.3006788/1‑130 (MQ=60)
                                             TCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGG                                                                                                          >  SRR19973061.2673731/1‑150 (MQ=60)
                                                CCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACC                                                                                                       <  SRR19973061.1236199/150‑1 (MQ=60)
                                                CCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACC                                                                                                       <  SRR19973061.619143/150‑1 (MQ=60)
                                                 CGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCATATTATTCTCTTCCTCATCCGCACAATTCTGACCG                                                                                                      >  SRR19973061.2382481/1‑150 (MQ=60)
                                                 CGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCG                                                                                                      >  SRR19973061.2382495/1‑150 (MQ=60)
                                                 CGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCG                                                                                                      >  SRR19973061.1963191/1‑150 (MQ=60)
                                                     GCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACAC                                                                                                  <  SRR19973061.2220409/150‑1 (MQ=60)
                                                         GGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCAC                                                                                              <  SRR19973061.36924/150‑1 (MQ=60)
                                                               AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTG                                                                                        <  SRR19973061.2410714/150‑1 (MQ=60)
                                                               AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTG                                                                                        <  SRR19973061.2411077/150‑1 (MQ=60)
                                                               AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTG                                                                                        <  SRR19973061.1163600/150‑1 (MQ=60)
                                                               AGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTG                                                                                        <  SRR19973061.2635771/150‑1 (MQ=60)
                                                                 CAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGA                                                                                      >  SRR19973061.3443479/1‑150 (MQ=60)
                                                                  AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                     >  SRR19973061.2935475/1‑150 (MQ=60)
                                                                  AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                     >  SRR19973061.939104/1‑150 (MQ=60)
                                                                  AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                     >  SRR19973061.1028235/1‑150 (MQ=60)
                                                                  AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                     >  SRR19973061.1028297/1‑150 (MQ=60)
                                                                  AGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAA                                                                                     <  SRR19973061.1239477/150‑1 (MQ=60)
                                                                   GAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAG                                                                                    <  SRR19973061.939067/150‑1 (MQ=60)
                                                                    ANGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGA                                                                                   >  SRR19973061.1400713/1‑150 (MQ=60)
                                                                     AGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAT                                                                                  <  SRR19973061.2660270/150‑1 (MQ=60)
                                                                     AGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGAT                                                                                  <  SRR19973061.3155782/150‑1 (MQ=60)
                                                                           TGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGC                                                                            <  SRR19973061.529118/150‑1 (MQ=60)
                                                                           TGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGC                                                                            >  SRR19973061.2505995/1‑150 (MQ=60)
                                                                            GATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCC                                                                           <  SRR19973061.1010004/150‑1 (MQ=60)
                                                                             ATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCG                                                                          <  SRR19973061.3705370/150‑1 (MQ=60)
                                                                                     GTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTA                                                                  <  SRR19973061.2737158/150‑1 (MQ=60)
                                                                                     GTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTA                                                                  >  SRR19973061.3654151/1‑150 (MQ=60)
                                                                                       TGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGC                                                                >  SRR19973061.1471449/1‑150 (MQ=60)
                                                                                             GTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAA                                                          <  SRR19973061.1959469/150‑1 (MQ=60)
                                                                                             GTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAA                                                          <  SRR19973061.1958730/150‑1 (MQ=60)
                                                                                              TTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAG                                                         <  SRR19973061.3560110/150‑1 (MQ=60)
                                                                                              TTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAG                                                         <  SRR19973061.2575258/150‑1 (MQ=60)
                                                                                                 CGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAATCCA                                                      >  SRR19973061.1919657/1‑150 (MQ=60)
                                                                                                    CGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACA                                                   >  SRR19973061.2359453/1‑150 (MQ=60)
                                                                                                    CGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACA                                                   >  SRR19973061.458455/1‑150 (MQ=60)
                                                                                                    CGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACA                                                   <  SRR19973061.1557130/150‑1 (MQ=60)
                                                                                                    CGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACA                                                   >  SRR19973061.458450/1‑150 (MQ=60)
                                                                                                       GGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCC                                                <  SRR19973061.293908/150‑1 (MQ=60)
                                                                                                       GGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCC                                                <  SRR19973061.336263/150‑1 (MQ=60)
                                                                                                           TAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGA                                            >  SRR19973061.2449064/1‑150 (MQ=60)
                                                                                                             GCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGC                                          <  SRR19973061.3620743/150‑1 (MQ=60)
                                                                                                                  CGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTT                                     <  SRR19973061.105898/150‑1 (MQ=60)
                                                                                                                   GATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTC                                    >  SRR19973061.2301373/1‑150 (MQ=60)
                                                                                                                      GGTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAG                                 <  SRR19973061.2736403/150‑1 (MQ=60)
                                                                                                                       GTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGA                                <  SRR19973061.3395003/150‑1 (MQ=60)
                                                                                                                       GTGTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGA                                <  SRR19973061.3394996/150‑1 (MQ=60)
                                                                                                                         GTCACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATG                              <  SRR19973061.2482256/150‑1 (MQ=60)
                                                                                                                           CACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCC                            <  SRR19973061.2098705/150‑1 (MQ=60)
                                                                                                                            ACGTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCT                           <  SRR19973061.1142709/150‑1 (MQ=60)
                                                                                                                              GTCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTT                         >  SRR19973061.1285663/1‑150 (MQ=60)
                                                                                                                               TCGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTC                        <  SRR19973061.414458/150‑1 (MQ=60)
                                                                                                                                CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                       >  SRR19973061.3640940/1‑150 (MQ=60)
                                                                                                                                CGTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCC                       >  SRR19973061.2652238/1‑150 (MQ=60)
                                                                                                                                 GTTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCA                      >  SRR19973061.3402737/1‑150 (MQ=60)
                                                                                                                                  TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                     >  SRR19973061.785053/1‑150 (MQ=60)
                                                                                                                                  TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                     <  SRR19973061.2670172/150‑1 (MQ=60)
                                                                                                                                  TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                     >  SRR19973061.3591946/1‑150 (MQ=60)
                                                                                                                                  TTTCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAG                     >  SRR19973061.3591881/1‑150 (MQ=60)
                                                                                                                                    TCATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAA                   >  SRR19973061.3720144/1‑150 (MQ=60)
                                                                                                                                     CATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAT                  <  SRR19973061.3395537/150‑1 (MQ=60)
                                                                                                                                     CATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAAT                  <  SRR19973061.2973547/150‑1 (MQ=60)
                                                                                                                                      ATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATT                 >  SRR19973061.1056981/1‑150 (MQ=60)
                                                                                                                                      ATTTGCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATT                 >  SRR19973061.1056928/1‑150 (MQ=60)
                                                                                                                                          GCGCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCAC             <  SRR19973061.1248026/150‑1 (MQ=60)
                                                                                                                                            GCTCCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGG           <  SRR19973061.2801083/150‑1 (MQ=60)
                                                                                                                                               CNTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT        >  SRR19973061.131073/1‑150 (MQ=60)
                                                                                                                                               CCTCGTATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGACGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTT        >  SRR19973061.1303913/1‑150 (MQ=60)
                                                                                                                                                     ATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCACAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  SRR19973061.1124684/1‑150 (MQ=60)
                                                                                                                                                     ATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  SRR19973061.1123968/1‑150 (MQ=60)
                                                                                                                                                     ATTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  SRR19973061.1123863/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTTGCATCAACCAGTTGAAGGACGGGAACAAAAAGCCCAGCCCGCTCACCGCCGCCAGGATGAAGCAGAAGGCGGTGATCCAGTGGTTGATACGTTCCGGCGCGGTGTAGCGCACGATGGTGTCACGTCGTTTCATTTGCGCTCCTCGTCTTTCTCTTCGTGCAGATTATTCTCTTCCTCATCCGCACGGTTCGGACCGACACCCACGTAGTGGAAGATACTGGCCGCAAAGGTAGCCGCAAAGCCAACAGCCGCGAGCGGTTTCCAGATGCCTTTCCAGAATTTCACGGTTTCGCTGA  >  CP009273/4075085‑4075383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑