Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,089 T→A 100% intergenic (‑120/‑310) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0890TA96.3% 685.9 / 10.7 215intergenic (‑120/‑310)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (1/7);  new base A (107/100);  total (108/107)
Fisher's exact test for biased strand distribution p-value = 3.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

ACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTA  >  CP009273/4264944‑4265232
                                                                                                                                                 |                                                                                                                                               
     gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTaa                                                                                                                                          <  2:2188445/148‑1 (MQ=255)
     gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTaa                                                                                                                                          >  2:696765/1‑148 (MQ=255)
     gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTaa                                                                                                                                          <  1:1960932/148‑1 (MQ=255)
        aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                                                       <  1:3481327/148‑1 (MQ=255)
         gtggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCCCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAtttt                                                                                                                                      <  2:2529891/148‑1 (MQ=255)
          tggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtg                                                                                                                                     >  2:1423712/1‑148 (MQ=255)
             tgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGa                                                                                                                                  >  2:3044601/1‑148 (MQ=255)
             tgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGa                                                                                                                                  >  2:2433703/1‑148 (MQ=255)
              gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGaa                                                                                                                                 <  2:2564576/148‑1 (MQ=255)
                gggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACt                                                                                                                               <  2:2914749/148‑1 (MQ=255)
                   tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtata                                                                                                                            <  2:3241065/148‑1 (MQ=255)
                   tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtata                                                                                                                            <  2:2895583/148‑1 (MQ=255)
                   tAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtata                                                                                                                            >  1:807548/1‑148 (MQ=255)
                      ccATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATca                                                                                                                         >  1:2551800/1‑148 (MQ=255)
                        aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcaca                                                                                                                       <  1:567960/148‑1 (MQ=255)
                         tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                                                      <  2:431442/148‑1 (MQ=255)
                         tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                                                      <  1:857075/148‑1 (MQ=255)
                         tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                                                      >  1:2113534/1‑148 (MQ=255)
                          cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAat                                                                                                                     >  1:2878051/1‑148 (MQ=255)
                          cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAat                                                                                                                     <  1:636535/148‑1 (MQ=255)
                           aaaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAatt                                                                                                                    >  2:1113901/1‑148 (MQ=255)
                            aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattg                                                                                                                   >  2:2068773/1‑148 (MQ=255)
                            aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattg                                                                                                                   <  2:1557458/148‑1 (MQ=255)
                             aCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattaa                                                                                                                  >  1:3531338/1‑146 (MQ=255)
                               aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgatt                                                                                                                >  1:3072138/1‑148 (MQ=255)
                                aTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgattg                                                                                                               >  2:2252109/1‑148 (MQ=255)
                                aTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgattg                                                                                                               >  2:47416/1‑148 (MQ=255)
                                 ttCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGAttgt                                                                                                              <  1:2734112/148‑1 (MQ=255)
                                   ccTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgttt                                                                                                            <  1:2520114/148‑1 (MQ=255)
                                      ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                                         <  1:1208674/148‑1 (MQ=255)
                                         aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                                                      <  1:2713865/148‑1 (MQ=255)
                                         aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                                                      >  2:553023/1‑148 (MQ=255)
                                           aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                                                    >  1:8724/1‑148 (MQ=255)
                                           aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                                                    >  1:1609361/1‑148 (MQ=255)
                                            agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCat                                                                                                   <  2:1562328/148‑1 (MQ=255)
                                            agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCat                                                                                                   >  1:2862311/1‑148 (MQ=255)
                                             gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                                                  >  1:1719597/1‑148 (MQ=255)
                                              agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                                                 >  1:2301653/1‑148 (MQ=255)
                                              agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                                                 >  1:2121076/1‑148 (MQ=255)
                                              agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                                                 >  1:908012/1‑148 (MQ=255)
                                              agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                                                 >  1:2937500/1‑148 (MQ=255)
                                                ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAGTGATTGTTTGTTAGCCATATTa                                                                                               >  2:3366701/1‑148 (MQ=255)
                                                  gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                                             >  1:2260786/1‑148 (MQ=255)
                                                  gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                                             <  1:293398/148‑1 (MQ=255)
                                                   aTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGc                                                                                            >  1:1387477/1‑148 (MQ=255)
                                                     gCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCg                                                                                          >  1:1287688/1‑148 (MQ=255)
                                                        cTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGa                                                                                       <  1:3441204/148‑1 (MQ=255)
                                                         tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAc                                                                                      >  1:1767231/1‑148 (MQ=255)
                                                         tGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAc                                                                                      >  1:1499559/1‑148 (MQ=255)
                                                          ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                                                     <  2:1979895/148‑1 (MQ=255)
                                                          ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATGAGGCCGTGACt                                                                                     >  1:2922336/1‑148 (MQ=255)
                                                           gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                                                       <  2:2549558/97‑1 (MQ=255)
                                                           gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTATGCCGTGACtt                                                                                    >  1:938306/1‑148 (MQ=255)
                                                            aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                   >  1:1977349/1‑148 (MQ=255)
                                                            aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                   <  2:1207971/148‑1 (MQ=255)
                                                            aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                   <  2:3137842/148‑1 (MQ=255)
                                                             cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                                                  >  2:2384740/1‑148 (MQ=255)
                                                               gcgcGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAt                                                                                <  2:1922748/148‑1 (MQ=255)
                                                                  cggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGc                                                                             >  2:1236061/1‑148 (MQ=255)
                                                                        tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACa                                                                       >  2:2712435/1‑148 (MQ=255)
                                                                         gttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAg                                                                      <  2:3485456/148‑1 (MQ=255)
                                                                           tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAt                                                                    >  2:3089777/1‑148 (MQ=255)
                                                                             aCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTa                                                                  <  1:730462/148‑1 (MQ=255)
                                                                                ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgt                                                               >  2:204243/1‑148 (MQ=255)
                                                                                  aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgttg                                                             <  2:737173/148‑3 (MQ=255)
                                                                                  aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                                             <  1:2612770/148‑1 (MQ=255)
                                                                                  aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                                             <  1:1348936/148‑1 (MQ=255)
                                                                                     cGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGttt                                                          <  2:18461/148‑1 (MQ=255)
                                                                                        ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                                       <  2:3074764/148‑1 (MQ=255)
                                                                                          tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                                     <  1:2019307/148‑1 (MQ=255)
                                                                                          tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                                     <  1:1423712/148‑1 (MQ=255)
                                                                                             acTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGATGTACAGATTATGTGGTTTTTCAGTgg                                                  >  2:3325983/1‑148 (MQ=255)
                                                                                                ctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAt                                               >  1:3428820/1‑148 (MQ=255)
                                                                                                ctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAt                                               <  2:234111/148‑1 (MQ=255)
                                                                                                  ggCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTa                                             <  2:3499901/148‑1 (MQ=255)
                                                                                                   gCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTaa                                            >  2:704065/1‑148 (MQ=255)
                                                                                                     tATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                                          <  2:508550/148‑1 (MQ=255)
                                                                                                      aTCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAggg                                         >  2:1636049/1‑148 (MQ=255)
                                                                                                      aTCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAGTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAggg                                         >  2:3409969/1‑148 (MQ=255)
                                                                                                       tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                        >  1:382240/1‑148 (MQ=255)
                                                                                                        cgcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCa                                       >  2:1138510/1‑148 (MQ=255)
                                                                                                                ttGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTcc                               >  1:3014624/1‑148 (MQ=255)
                                                                                                                     gAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgccg                          >  1:3114056/1‑148 (MQ=255)
                                                                                                                      aaaacaAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCcgccgc                         <  1:661054/145‑1 (MQ=255)
                                                                                                                       aaacaAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCgccgcc                        >  1:2155100/3‑148 (MQ=255)
                                                                                                                          caAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCctct                     >  1:240975/1‑148 (MQ=255)
                                                                                                                          caAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCctct                     >  1:3364371/1‑148 (MQ=255)
                                                                                                                              aaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAt                 >  1:3513475/1‑148 (MQ=255)
                                                                                                                               aTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAtt                >  2:742118/1‑148 (MQ=255)
                                                                                                                               aTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAtt                >  2:2733896/1‑148 (MQ=255)
                                                                                                                               aTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAtt                >  1:1920277/1‑148 (MQ=255)
                                                                                                                               aTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAtt                >  1:1921555/1‑148 (MQ=255)
                                                                                                                                 gAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTcc              <  1:3119152/148‑1 (MQ=255)
                                                                                                                                 gAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTcc              <  1:1188815/148‑1 (MQ=255)
                                                                                                                                 gAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTcc              <  1:3227833/148‑1 (MQ=255)
                                                                                                                                         ctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGaa      <  2:1719597/148‑1 (MQ=255)
                                                                                                                                          tgtttgtaTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGaaa     >  2:1692153/1‑148 (MQ=255)
                                                                                                                                           gtttgtatCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaat    >  1:3337978/1‑148 (MQ=255)
                                                                                                                                            tttgtatCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaatt   >  2:622977/1‑148 (MQ=255)
                                                                                                                                             ttgtatCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaatta  <  2:1333374/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTA  >  CP009273/4264944‑4265232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  >  CP009273/4264942‑4265238
                                                                                                                                                   |                                                                                                                                                     
GAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATC                                                                                                                                                     >  SRR19973067.2707662/1‑150 (MQ=60)
 AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCA                                                                                                                                                    <  SRR19973067.2841156/150‑1 (MQ=60)
      CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                                               <  SRR19973067.1678754/150‑1 (MQ=60)
       GCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATT                                                                                                                                              <  SRR19973067.1974258/150‑1 (MQ=60)
         CAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTT                                                                                                                                            >  SRR19973067.2942166/1‑150 (MQ=60)
         CAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTT                                                                                                                                            >  SRR19973067.2942163/1‑150 (MQ=60)
          AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTG                                                                                                                                           <  SRR19973067.3503419/150‑1 (MQ=60)
          AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTG                                                                                                                                           >  SRR19973067.2979519/1‑150 (MQ=60)
            TGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTG                                                                                                                                         <  SRR19973067.2318614/150‑1 (MQ=60)
            TGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTG                                                                                                                                         <  SRR19973067.2128349/150‑1 (MQ=60)
                 AGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTA                                                                                                                                    >  SRR19973067.3441333/1‑150 (MQ=60)
                   GGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATA                                                                                                                                  >  SRR19973067.813099/1‑150 (MQ=60)
                   GGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATA                                                                                                                                  <  SRR19973067.2859663/150‑1 (MQ=60)
                      AGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCA                                                                                                                               >  SRR19973067.3198923/1‑150 (MQ=60)
                      AGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCA                                                                                                                               >  SRR19973067.2568489/1‑150 (MQ=60)
                       GCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCAC                                                                                                                              <  SRR19973067.365493/150‑1 (MQ=60)
                         CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAA                                                                                                                            >  SRR19973067.2127608/1‑150 (MQ=60)
                          ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAAT                                                                                                                           <  SRR19973067.1197617/150‑1 (MQ=60)
                          ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                                           <  SRR19973067.571654/150‑1 (MQ=60)
                          ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                                           >  SRR19973067.2896464/1‑150 (MQ=60)
                          ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                                           >  SRR19973067.2894640/1‑150 (MQ=60)
                           TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                                          <  SRR19973067.2655319/150‑1 (MQ=60)
                           TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                                          <  SRR19973067.1933767/150‑1 (MQ=60)
                           TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                                          <  SRR19973067.862842/150‑1 (MQ=60)
                            CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTG                                                                                                                         <  SRR19973067.640813/150‑1 (MQ=60)
                             AAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGA                                                                                                                        >  SRR19973067.2010332/1‑150 (MQ=60)
                             AAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTAA                                                                                                                        >  SRR19973067.3553661/1‑150 (MQ=60)
                               ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATT                                                                                                                      >  SRR19973067.3091694/1‑150 (MQ=60)
                                CAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTG                                                                                                                     >  SRR19973067.235773/1‑150 (MQ=60)
                                   TTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                                                  <  SRR19973067.2751727/150‑1 (MQ=60)
                                   TTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                                                  <  SRR19973067.2751759/150‑1 (MQ=60)
                                     CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                                                <  SRR19973067.2536639/150‑1 (MQ=60)
                                     CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                                                >  SRR19973067.570288/1‑150 (MQ=60)
                                        CTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAG                                                                                                             <  SRR19973067.1970274/150‑1 (MQ=60)
                                        CTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAG                                                                                                             <  SRR19973067.1216619/150‑1 (MQ=60)
                                          GAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCC                                                                                                           <  SRR19973067.1409615/150‑1 (MQ=60)
                                          GAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCC                                                                                                           <  SRR19973067.1409569/150‑1 (MQ=60)
                                           AATAGAGCCGATGCCCTGGACAGCGCGTCGTTTTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                                          <  SRR19973067.942237/150‑1 (MQ=60)
                                           AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                                          <  SRR19973067.2731355/150‑1 (MQ=60)
                                           AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                                          >  SRR19973067.1620413/1‑150 (MQ=60)
                                           AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                                          >  SRR19973067.8861/1‑150 (MQ=60)
                                            AAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAT                                                                                                         >  SRR19973067.2880655/1‑150 (MQ=60)
                                             AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                        >  SRR19973067.1731375/1‑150 (MQ=60)
                                              AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACCCTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                                                       <  SRR19973067.1765828/150‑1 (MQ=60)
                                              AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                                                       >  SRR19973067.2316823/1‑150 (MQ=60)
                                              AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                                                       >  SRR19973067.2135223/1‑150 (MQ=60)
                                              AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                                                       >  SRR19973067.2956133/1‑150 (MQ=60)
                                              AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATAACAATTGATTGTTTGTTAGCCATAT                                                                                                       >  SRR19973067.914025/1‑150 (MQ=60)
                                                 GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                                                    >  SRR19973067.3542450/1‑150 (MQ=60)
                                                 GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                                                    <  SRR19973067.2432911/150‑1 (MQ=60)
                                                  CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGTGCTTGCAGAAAACAAATATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACTATTGATTGTTTGTTATCCATATTAGG                                                                                                   <  SRR19973067.3531372/150‑1 (MQ=60)
                                                  CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGG                                                                                                   >  SRR19973067.2275785/1‑150 (MQ=60)
                                                   CGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGC                                                                                                  >  SRR19973067.1396914/1‑150 (MQ=60)
                                                    GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                                                 <  SRR19973067.595896/150‑1 (MQ=60)
                                                    GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                                                 <  SRR19973067.295282/150‑1 (MQ=60)
                                                    GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                                                 <  SRR19973067.295338/150‑1 (MQ=60)
                                                    GATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                                                 >  SRR19973067.2146068/1‑150 (MQ=60)
                                                     ATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCG                                                                                                >  SRR19973067.1296473/1‑150 (MQ=60)
                                                         CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAC                                                                                            >  SRR19973067.1509439/1‑150 (MQ=60)
                                                         CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAC                                                                                            >  SRR19973067.1509036/1‑150 (MQ=60)
                                                         CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGGGAC                                                                                            >  SRR19973067.1779235/1‑150 (MQ=60)
                                                          CTGGACAGCGCGGCGTTGTTCACGGGATGCGTGGTACACTCTTGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTTAAATATATCACAAGTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                                           >  SRR19973067.2940692/1‑150 (MQ=60)
                                                          CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                                           <  SRR19973067.3463086/150‑1 (MQ=60)
                                                          CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                                           >  SRR19973067.2586543/1‑150 (MQ=60)
                                                          CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                                           <  SRR19973067.3463107/150‑1 (MQ=60)
                                                          CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                                           <  SRR19973067.3463082/150‑1 (MQ=60)
                                                          CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATGAGGCCGTGACT                                                                                           >  SRR19973067.2940913/1‑150 (MQ=60)
                                                           TGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGagatcggaagagcacacgtctgaactccagtcacgtattctcgcatctc                                                                                          >  SRR19973067.2566237/1‑101 (MQ=60)
                                                           TGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTATGCCGTGACTT                                                                                          >  SRR19973067.944685/1‑150 (MQ=60)
                                                            GGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTT                                                                                         >  SRR19973067.1990743/1‑150 (MQ=60)
                                                                AGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATT                                                                                     >  SRR19973067.86317/1‑150 (MQ=60)
                                                                 GCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGCGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTG                                                                                    <  SRR19973067.901372/150‑1 (MQ=60)
                                                                         TTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAG                                                                            <  SRR19973067.3330741/150‑1 (MQ=60)
                                                                           GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                                          <  SRR19973067.2089143/150‑1 (MQ=60)
                                                                               ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                                                      <  SRR19973067.735537/150‑1 (MQ=60)
                                                                               ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                                                      <  SRR19973067.735538/150‑1 (MQ=60)
                                                                                    ATGCGTGGTACACTCTGGCTATCGAGGGTCTTTCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                                                 <  SRR19973067.3135465/150‑1 (MQ=60)
                                                                                    AGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                                                 <  SRR19973067.2629727/150‑1 (MQ=60)
                                                                                    AGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                                                 <  SRR19973067.1358157/150‑1 (MQ=60)
                                                                                    AGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTT                                                                 <  SRR19973067.779753/150‑1 (MQ=60)
                                                                                           GTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGT                                                          <  SRR19973067.1834654/150‑1 (MQ=60)
                                                                                            TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                                         <  SRR19973067.224256/150‑1 (MQ=60)
                                                                                            TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                                         <  SRR19973067.1433327/150‑1 (MQ=60)
                                                                                            TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                                         <  SRR19973067.2032872/150‑1 (MQ=60)
                                                                                              CACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGC                                                       >  SRR19973067.66247/1‑150 (MQ=60)
                                                                                                CTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAT                                                     >  SRR19973067.3450638/1‑150 (MQ=60)
                                                                                                      CTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGG                                               >  SRR19973067.1311767/1‑150 (MQ=60)
                                                                                                       TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                                              >  SRR19973067.3137826/1‑150 (MQ=60)
                                                                                                       TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                                              >  SRR19973067.384903/1‑150 (MQ=60)
                                                                                                       TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                                              >  SRR19973067.384902/1‑150 (MQ=60)
                                                                                                                GCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCC                                     >  SRR19973067.3033804/1‑150 (MQ=60)
                                                                                                                  TTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCG                                   <  SRR19973067.312696/150‑1 (MQ=60)
                                                                                                                   TGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGC                                  >  SRR19973067.2307358/1‑150 (MQ=60)
                                                                                                                     CAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG                                <  SRR19973067.1864144/150‑1 (MQ=60)
                                                                                                                     CAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCG                                >  SRR19973067.3133850/1‑150 (MQ=60)
                                                                                                                       GAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCC                              >  SRR19973067.2169506/1‑150 (MQ=60)
                                                                                                                        AAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCT                             <  SRR19973067.665554/150‑1 (MQ=60)
                                                                                                                          AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                           >  SRR19973067.242654/1‑150 (MQ=60)
                                                                                                                          AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                           >  SRR19973067.1615000/1‑150 (MQ=60)
                                                                                                                          AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                           >  SRR19973067.3385727/1‑150 (MQ=60)
                                                                                                                          AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                           <  SRR19973067.1245320/150‑1 (MQ=60)
                                                                                                                          AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGAGAACTATATAACAATTGATTGTTTGTTAGCCATATTAGGCAGTGACTTTTATTGCTCTACAGATTATGTGGTTTTTCAGTGGCATTAAGGTCATATCTTCACGCCGCCTCT                           >  SRR19973067.873902/1‑150 (MQ=60)
                                                                                                                              AAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAT                       >  SRR19973067.3535723/1‑150 (MQ=60)
                                                                                                                              AAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAT                       <  SRR19973067.1983937/150‑1 (MQ=60)
                                                                                                                              AAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAT                       >  SRR19973067.139347/1‑150 (MQ=60)
                                                                                                                               AAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATT                      >  SRR19973067.1934663/1‑150 (MQ=60)
                                                                                                                               AAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATT                      >  SRR19973067.1933378/1‑150 (MQ=60)
                                                                                                                                  TGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                   <  SRR19973067.3497082/150‑1 (MQ=60)
                                                                                                                                  TGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                   >  SRR19973067.3089804/1‑150 (MQ=60)
                                                                                                                                  TGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                   <  SRR19973067.674334/150‑1 (MQ=60)
                                                                                                                                   GAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTG                  <  SRR19973067.3138984/150‑1 (MQ=60)
                                                                                                                                   GAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTG                  <  SRR19973067.3248098/150‑1 (MQ=60)
                                                                                                                                   GAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTG                  <  SRR19973067.1196638/150‑1 (MQ=60)
                                                                                                                                       CACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGG              <  SRR19973067.2073569/150‑1 (MQ=60)
                                                                                                                                           CTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAAT          >  SRR19973067.3359113/1‑150 (MQ=60)
                                                                                                                                           CTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAAT          >  SRR19973067.3359114/1‑150 (MQ=60)
                                                                                                                                                TGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATT     >  SRR19973067.665912/1‑150 (MQ=60)
                                                                                                                                                TGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATT     >  SRR19973067.665919/1‑150 (MQ=60)
                                                                                                                                                  TATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT   <  SRR19973067.1071276/150‑1 (MQ=60)
                                                                                                                                                  TATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT   <  SRR19973067.1070966/150‑1 (MQ=60)
                                                                                                                                                   ATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  <  SRR19973067.208908/150‑1 (MQ=60)
                                                                                                                                                   ATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  <  SRR19973067.1361954/150‑1 (MQ=60)
                                                                                                                                                   ATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  <  SRR19973067.1361959/150‑1 (MQ=60)
                                                                                                                                                   ATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  >  SRR19973067.2848444/1‑150 (MQ=60)
                                                                                                                                                   ATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  >  SRR19973067.1061415/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTG  >  CP009273/4264942‑4265238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑