Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,089 T→A 100% intergenic (‑120/‑310) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0890TA98.3% 774.1 / 1.7 237intergenic (‑120/‑310)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (109/124);  total (112/125)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 244 total aligned reads displayed.

CCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAAT  >  CP009273/4264945‑4265234
                                                                                                                                                |                                                                                                                                                 
ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                                                <  2:2079909/148‑1 (MQ=255)
ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                                                <  1:2330410/148‑1 (MQ=255)
    gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTaa                                                                                                                                            <  2:243886/148‑1 (MQ=255)
    gCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTaa                                                                                                                                            >  2:3170812/1‑148 (MQ=255)
     ccAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAt                                                                                                                                           <  2:951738/148‑1 (MQ=255)
       aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                                                         <  1:2517905/148‑1 (MQ=255)
       aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                                                         <  2:2984993/148‑1 (MQ=255)
         tggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtg                                                                                                                                       <  1:2870646/148‑1 (MQ=255)
             gAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGaa                                                                                                                                   <  1:501259/148‑1 (MQ=255)
                ggTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACta                                                                                                                                >  1:3210888/1‑148 (MQ=255)
                   aGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTatat                                                                                                                             >  2:2821796/1‑148 (MQ=255)
                    gCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATc                                                                                                                            >  2:1774218/1‑148 (MQ=255)
                    gCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATc                                                                                                                            >  2:2347512/1‑148 (MQ=255)
                      cATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcac                                                                                                                          >  1:1035919/1‑148 (MQ=255)
                        tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATAACACaa                                                                                                                        <  2:712969/148‑1 (MQ=255)
                         cAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAat                                                                                                                       >  2:2714735/1‑148 (MQ=255)
                           aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattg                                                                                                                     <  2:1306083/148‑1 (MQ=255)
                            aCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattga                                                                                                                    <  1:81709/148‑1 (MQ=255)
                             cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                                                   >  1:918619/1‑148 (MQ=255)
                             cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                                                   >  1:3052747/1‑148 (MQ=255)
                              aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgatt                                                                                                                  <  2:3005922/148‑1 (MQ=255)
                               aTTACTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCTCGGGAGGCGTGGTACACTCTGGCTATCGAGGGGCTTGCATAAAACAAAAATGATACACTCTGTTTTTATCATTAATTTTTTGAACTATATCACAattgattg                                                                                                                 <  2:289515/148‑1 (MQ=255)
                                ttCCTCTGAATAGAGCCGATGCCCTGGACAGAGCGGCTTTGTTCTGGGGAGGCGTTGTTAACTCTTGCTTTCGCGGGGATTGCAGTAAACTTAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTTTATCTCAATTGAttgt                                                                                                                <  1:1164193/148‑1 (MQ=255)
                                ttCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgt                                                                                                                <  2:1331911/148‑1 (MQ=255)
                                 tCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtt                                                                                                               >  1:2156877/1‑148 (MQ=255)
                                   ctctGAAAAGAGCCGATGCCCTGGATAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttg                                                                                                             <  2:563012/148‑1 (MQ=255)
                                   ctctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttg                                                                                                             <  1:3478897/148‑1 (MQ=255)
                                     ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                                           >  2:86497/1‑148 (MQ=255)
                                     ctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATtgtttgtt                                                                                                           <  2:1920086/148‑1 (MQ=255)
                                          aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                                                      <  1:2962210/148‑1 (MQ=255)
                                          aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                                                      <  1:1881570/148‑1 (MQ=255)
                                          aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                                                      <  1:74763/148‑1 (MQ=255)
                                           agagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCat                                                                                                     <  1:2262526/148‑1 (MQ=255)
                                            gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                                                    <  1:1774218/148‑1 (MQ=255)
                                            gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                                                    >  2:2263154/1‑148 (MQ=255)
                                            gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                                                    >  2:939680/1‑148 (MQ=255)
                                             agCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCatat                                                                                                   >  1:2678294/1‑148 (MQ=255)
                                              gCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAtt                                                                                                  <  2:2497625/148‑1 (MQ=255)
                                               ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTa                                                                                                 <  1:2185791/148‑1 (MQ=255)
                                                cGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAg                                                                                                <  2:1963664/148‑1 (MQ=255)
                                                 gATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                                               <  2:442339/148‑1 (MQ=255)
                                                      ccTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAgg                                                                                               <  1:1892263/143‑1 (MQ=255)
                                                       cTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGa                                                                                         >  2:1876346/1‑148 (MQ=255)
                                                         ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                                                       >  2:1234154/1‑148 (MQ=255)
                                                         ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                                                       <  2:3425468/148‑1 (MQ=255)
                                                          gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                                      <  2:1845117/148‑1 (MQ=255)
                                                           aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                     <  1:1126133/148‑1 (MQ=255)
                                                           aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                     <  1:376060/148‑1 (MQ=255)
                                                           aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                     <  1:3401544/148‑1 (MQ=255)
                                                             aGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTa                                                                                   >  1:354118/1‑148 (MQ=255)
                                                             aGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTa                                                                                   >  2:1684305/1‑148 (MQ=255)
                                                              gcgcGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAt                                                                                  <  1:940645/148‑1 (MQ=255)
                                                               cgcgGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAtt                                                                                 >  2:2323936/1‑148 (MQ=255)
                                                                  ggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                                              <  2:2637552/148‑1 (MQ=255)
                                                                       tgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAGTTTTATTGCTGTACa                                                                         >  2:2013576/1‑148 (MQ=255)
                                                                           cACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAtt                                                                     <  1:3102876/148‑1 (MQ=255)
                                                                            aCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTa                                                                    <  1:1794824/148‑1 (MQ=255)
                                                                            aCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTa                                                                    <  1:561182/148‑1 (MQ=255)
                                                                             cGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAt                                                                   <  2:2764563/148‑1 (MQ=255)
                                                                              gggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtg                                                                  <  1:787772/148‑1 (MQ=255)
                                                                                gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                                                >  2:2378474/1‑148 (MQ=255)
                                                                                gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                                                <  1:1302608/148‑1 (MQ=255)
                                                                                gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                                                >  2:3067005/1‑148 (MQ=255)
                                                                                gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                                                <  1:2714735/148‑1 (MQ=255)
                                                                                    cGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGttt                                                            <  1:2347512/148‑1 (MQ=255)
                                                                                         tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                                       <  2:2156877/148‑1 (MQ=255)
                                                                                         tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                                       <  2:39104/148‑1 (MQ=255)
                                                                                         tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                                       >  1:2858376/1‑148 (MQ=255)
                                                                                          acacTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGt                                                      <  1:1606783/148‑1 (MQ=255)
                                                                                           cacTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAGTATGTGGTTTTTCAGTg                                                     >  2:293569/1‑148 (MQ=255)
                                                                                             ctctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGc                                                   >  2:1747376/1‑148 (MQ=255)
                                                                                              tctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCa                                                  <  2:2240374/148‑1 (MQ=255)
                                                                                                   cTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTGAg                                             <  2:1542393/148‑1 (MQ=255)
                                                                                                    tATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                                            <  1:2494380/148‑1 (MQ=255)
                                                                                                     aTCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAggg                                           >  2:3067178/1‑148 (MQ=255)
                                                                                                      tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                          >  2:633936/1‑148 (MQ=255)
                                                                                                      tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                          >  1:2062365/1‑148 (MQ=255)
                                                                                                      tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                          >  2:651917/1‑148 (MQ=255)
                                                                                                      tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                          >  2:1433071/1‑148 (MQ=255)
                                                                                                        gcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCat                                        <  1:1965483/148‑1 (MQ=255)
                                                                                                           gggCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATc                                     >  2:2032497/1‑148 (MQ=255)
                                                                                                              cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc                                  <  2:1344151/148‑1 (MQ=255)
                                                                                                                  cAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgc                              >  2:2164143/1‑148 (MQ=255)
                                                                                                                    gAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgccg                            >  2:313725/1‑148 (MQ=255)
                                                                                                                      aaacaAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCgccgcc                          >  1:712708/3‑148 (MQ=255)
                                                                                                                          aaaaaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTg                      >  2:556607/1‑148 (MQ=255)
                                                                                                                          aaaaaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAGTAAGGGCATATCTTCCCGCCGCCTCTg                      >  2:2347259/1‑148 (MQ=255)
                                                                                                                             aaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAt                   <  2:3186944/148‑1 (MQ=255)
                                                                                                                             aaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAt                   <  2:3378762/148‑1 (MQ=255)
                                                                                                                               tGACACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTCTGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTc                 <  1:1745583/148‑1 (MQ=255)
                                                                                                                               tGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTc                 <  1:3170812/148‑1 (MQ=255)
                                                                                                                                   acacTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGt             <  2:1278935/148‑1 (MQ=255)
                                                                                                                                     acTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAg           >  2:1940713/1‑148 (MQ=255)
                                                                                                                                     acTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAg           <  1:1584034/148‑1 (MQ=255)
                                                                                                                                       tctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGa         >  2:1184941/1‑148 (MQ=255)
                                                                                                                                       tctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGa         >  2:2058476/1‑148 (MQ=255)
                                                                                                                                       tctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTGTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGa         >  2:1020431/1‑148 (MQ=255)
                                                                                                                                          gtttgtatCATTAATTTTGTGAACTATATAACAATTGATTGTTTGTTAGCCATATTATGCCGTGACTTTTATTGTTTTACAGATTATTTGGTTTTTCAGTGGCATTAATGTCATATCTTCCCGCCGCCTCTGCATTCCTGTATGAaat      >  1:2948522/1‑148 (MQ=255)
                                                                                                                                           tttgtttCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaatt     >  2:3313155/1‑148 (MQ=255)
                                                                                                                                              gtttCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaattaat  <  1:1234154/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAAT  >  CP009273/4264945‑4265234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT  >  CP009273/4264941‑4265237
                                                                                                                                                    |                                                                                                                                                    
AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTAT                                                                                                                                                     <  SRR19973066.1423453/150‑1 (MQ=60)
  AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCA                                                                                                                                                   <  SRR19973066.2012214/150‑1 (MQ=60)
  AACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCA                                                                                                                                                   <  SRR19973066.2012174/150‑1 (MQ=60)
    CCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATT                                                                                                                                                 <  SRR19973066.2346317/150‑1 (MQ=60)
        GCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATT                                                                                                                                             <  SRR19973066.2593645/150‑1 (MQ=60)
           AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTTGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTG                                                                                                                                          >  SRR19973066.1951895/1‑150 (MQ=60)
           AGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTG                                                                                                                                          <  SRR19973066.2535028/150‑1 (MQ=60)
             TGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTG                                                                                                                                        <  SRR19973066.2889354/150‑1 (MQ=60)
             TGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTG                                                                                                                                        <  SRR19973066.2889898/150‑1 (MQ=60)
                 GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACT                                                                                                                                    <  SRR19973066.504983/150‑1 (MQ=60)
                 GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACT                                                                                                                                    <  SRR19973066.917230/150‑1 (MQ=60)
                 GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACT                                                                                                                                    <  SRR19973066.917242/150‑1 (MQ=60)
                 GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACT                                                                                                                                    >  SRR19973066.3208139/1‑150 (MQ=60)
                 GAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACT                                                                                                                                    >  SRR19973066.3207958/1‑150 (MQ=60)
                  AGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTA                                                                                                                                   >  SRR19973066.3232169/1‑150 (MQ=60)
                    GGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATA                                                                                                                                 <  SRR19973066.759393/150‑1 (MQ=60)
                      TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                                               >  SRR19973066.381096/1‑150 (MQ=60)
                        GCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCAC                                                                                                                             >  SRR19973066.1043540/1‑150 (MQ=60)
                        GCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCAC                                                                                                                             >  SRR19973066.1201757/1‑150 (MQ=60)
                               AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                                                      >  SRR19973066.3073164/1‑150 (MQ=60)
                               AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                                                      >  SRR19973066.3080064/1‑150 (MQ=60)
                               AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                                                      >  SRR19973066.925214/1‑150 (MQ=60)
                               AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATAACAATTTAT                                                                                                                      >  SRR19973066.925351/1‑150 (MQ=60)
                                ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATT                                                                                                                     <  SRR19973066.81970/150‑1 (MQ=60)
                                ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATT                                                                                                                     <  SRR19973066.82635/150‑1 (MQ=60)
                                  AATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGT                                                                                                                   >  SRR19973066.1832088/1‑150 (MQ=60)
                                   ATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTT                                                                                                                  >  SRR19973066.2171857/1‑150 (MQ=60)
                                    TTCCTCTGAATAGAGCCGATGCCCTGGACAGAGCGGCTTTGTTCTGGGGAGGCGTTGTTAACTCTTGCTTTCGCGGGGATTGCAGTAAACTTAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTTTATCTCAATTGATTGTTT                                                                                                                 <  SRR19973066.1172441/150‑1 (MQ=53)
                                    TTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                                                 <  SRR19973066.2673047/150‑1 (MQ=60)
                                    GTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTT                                                                                                                 <  SRR19973066.2731277/150‑1 (MQ=60)
                                     TCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTG                                                                                                                <  SRR19973066.1848810/150‑1 (MQ=60)
                                     TCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTG                                                                                                                <  SRR19973066.1129136/150‑1 (MQ=60)
                                       CTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTT                                                                                                              <  SRR19973066.3502114/150‑1 (MQ=60)
                                        TCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTA                                                                                                             <  SRR19973066.1786724/150‑1 (MQ=60)
                                             AAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAT                                                                                                        >  SRR19973066.750841/1‑150 (MQ=60)
                                              AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                       <  SRR19973066.1895001/150‑1 (MQ=60)
                                              AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                       <  SRR19973066.2981844/150‑1 (MQ=60)
                                              AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                       <  SRR19973066.2981822/150‑1 (MQ=60)
                                              AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                       <  SRR19973066.75640/150‑1 (MQ=60)
                                               AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                                                      <  SRR19973066.2278103/150‑1 (MQ=60)
                                               AGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATAT                                                                                                      >  SRR19973066.2696172/1‑150 (MQ=60)
                                                GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                                                     <  SRR19973066.1786910/150‑1 (MQ=60)
                                                GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                                                     <  SRR19973066.1641346/150‑1 (MQ=60)
                                                   CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGG                                                                                                  <  SRR19973066.2200959/150‑1 (MQ=60)
                                                     tcTGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCC                                                                                                <  SRR19973066.1905754/148‑1 (MQ=60)
                                                         CCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGA                                                                                            >  SRR19973066.3142043/1‑150 (MQ=60)
                                                          CCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAC                                                                                           >  SRR19973066.1704490/1‑150 (MQ=60)
                                                           CTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACT                                                                                          <  SRR19973066.2149770/150‑1 (MQ=60)
                                                            TGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTT                                                                                         >  SRR19973066.2774068/1‑150 (MQ=60)
                                                               ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTA                                                                                      <  SRR19973066.1134510/150‑1 (MQ=60)
                                                               ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTA                                                                                      <  SRR19973066.1134229/150‑1 (MQ=60)
                                                               ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTA                                                                                      <  SRR19973066.3424253/150‑1 (MQ=60)
                                                               ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTA                                                                                      <  SRR19973066.379014/150‑1 (MQ=60)
                                                               ACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTGTTA                                                                                      >  SRR19973066.356872/1‑150 (MQ=60)
                                                                CAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAT                                                                                     <  SRR19973066.234858/150‑1 (MQ=60)
                                                                CAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTAT                                                                                     <  SRR19973066.2123194/150‑1 (MQ=60)
                                                                  GCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTG                                                                                   <  SRR19973066.947498/150‑1 (MQ=60)
                                                                  GCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTG                                                                                   <  SRR19973066.3541388/150‑1 (MQ=60)
                                                                  GCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTG                                                                                   <  SRR19973066.2744577/150‑1 (MQ=60)
                                                                    GCGGCGTTGTTCACGGGAGGCGTTGTACACTCTTGCTATCGCGGTGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGCTTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCT                                                                                 <  SRR19973066.1899541/150‑1 (MQ=60)
                                                                       GCGTTGTTCACGTGAGGCGGTGTACACTCTGGCTATCGCGGGTCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTA                                                                              <  SRR19973066.1597206/150‑1 (MQ=60)
                                                                            GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                                         <  SRR19973066.470853/150‑1 (MQ=60)
                                                                              TCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTA                                                                       <  SRR19973066.2412671/150‑1 (MQ=60)
                                                                               CACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAT                                                                      <  SRR19973066.3123601/150‑1 (MQ=60)
                                                                               CACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAT                                                                      <  SRR19973066.3123600/150‑1 (MQ=60)
                                                                                ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                                                     <  SRR19973066.2513715/150‑1 (MQ=60)
                                                                                ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                                                     <  SRR19973066.1807592/150‑1 (MQ=60)
                                                                                ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                                                     <  SRR19973066.565187/150‑1 (MQ=60)
                                                                                ACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATG                                                                     <  SRR19973066.565192/150‑1 (MQ=60)
                                                                                  GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                                                   >  SRR19973066.2193137/1‑150 (MQ=60)
                                                                                  GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                                                   <  SRR19973066.793578/150‑1 (MQ=60)
                                                                                    GAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGT                                                                 <  SRR19973066.1312048/150‑1 (MQ=60)
                                                                                    GAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGT                                                                 <  SRR19973066.2732970/150‑1 (MQ=60)
                                                                                        CGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTT                                                             <  SRR19973066.2363416/150‑1 (MQ=60)
                                                                                        CGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTT                                                             <  SRR19973066.2363476/150‑1 (MQ=60)
                                                                                        CGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTT                                                             <  SRR19973066.3497248/150‑1 (MQ=60)
                                                                                        CGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTT                                                             <  SRR19973066.3497249/150‑1 (MQ=60)
                                                                                           GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                          >  SRR19973066.2877577/1‑150 (MQ=60)
                                                                                              ACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGG                                                       <  SRR19973066.1618439/150‑1 (MQ=60)
                                                                                                 CTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAT                                                    <  SRR19973066.291364/150‑1 (MQ=60)
                                                                                                 CTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAT                                                    <  SRR19973066.533843/150‑1 (MQ=60)
                                                                                                       CTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATTATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGG                                              >  SRR19973066.376125/1‑150 (MQ=60)
                                                                                                        TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                                             <  SRR19973066.2511384/150‑1 (MQ=60)
                                                                                                        TATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGC                                             >  SRR19973066.2076657/1‑150 (MQ=60)
                                                                                                            GCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATAT                                         <  SRR19973066.1979370/150‑1 (MQ=60)
                                                                                                             CGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATC                                        >  SRR19973066.761537/1‑150 (MQ=60)
                                                                                                                GGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTC                                     <  SRR19973066.692301/150‑1 (MQ=60)
                                                                                                                  CTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCC                                   >  SRR19973066.1186295/1‑150 (MQ=60)
                                                                                                                  CTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCC                                   <  SRR19973066.1443294/150‑1 (MQ=60)
                                                                                                                        GAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCC                             >  SRR19973066.717988/1‑150 (MQ=60)
                                                                                                                           AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                          >  SRR19973066.2939101/1‑150 (MQ=60)
                                                                                                                             CAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGC                        >  SRR19973066.1372448/1‑150 (MQ=60)
                                                                                                                              AAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCA                       >  SRR19973066.3284946/1‑150 (MQ=60)
                                                                                                                              AAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCA                       >  SRR19973066.2434185/1‑150 (MQ=60)
                                                                                                                               AAAATGAAACACTCGGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCAT                      >  SRR19973066.1965401/1‑150 (MQ=60)
                                                                                                                                   TGACACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTCTGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                  <  SRR19973066.1758153/150‑1 (MQ=60)
                                                                                                                                   TGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                  <  SRR19973066.3191929/150‑1 (MQ=60)
                                                                                                                                   TGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                  <  SRR19973066.1246328/150‑1 (MQ=60)
                                                                                                                                     AAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGT                <  SRR19973066.225453/150‑1 (MQ=60)
                                                                                                                                     AAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGT                <  SRR19973066.225278/150‑1 (MQ=60)
                                                                                                                                      AACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTA               >  SRR19973066.1806462/1‑150 (MQ=60)
                                                                                                                                         ACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGA            <  SRR19973066.1595487/150‑1 (MQ=60)
                                                                                                                                            CTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGTTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAAT         >  SRR19973066.2968610/1‑150 (MQ=60)
                                                                                                                                            CTGTTTGTATCATTAATTTTGTGAACTATATAACAATTGATTGTTTGTTAGCCATATTATGCCGTGACTTTTATTGTTTTACAGATTATTTGGTTTTTCAGTGGCATTAATGTCATATCTTCCCGCCGCCTCTGCATTCCTGTATGAAAT         >  SRR19973066.2968088/1‑150 (MQ=60)
                                                                                                                                              GTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTA       >  SRR19973066.1986809/1‑150 (MQ=60)
                                                                                                                                                  GTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTT   <  SRR19973066.1242923/150‑1 (MQ=60)
                                                                                                                                                   TATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT  <  SRR19973066.2881580/150‑1 (MQ=60)
                                                                                                                                                   TATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT  >  SRR19973066.1994471/1‑150 (MQ=60)
                                                                                                                                                   TATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT  <  SRR19973066.1064177/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTT  >  CP009273/4264941‑4265237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑