Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,265,089 T→A 100% intergenic (‑120/‑310) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,265,0890TA99.1% 692.4 / ‑2.6 211intergenic (‑120/‑310)yjcB/yjcCputative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (90/119);  total (91/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

ACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAA  >  CP009273/4264944‑4265233
                                                                                                                                                 |                                                                                                                                                
aCCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTc                                                                                                                                                >  1:1562108/1‑148 (MQ=255)
 ccACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                                               >  2:1847819/1‑148 (MQ=255)
 ccACGCCAGTGGTGAGGGTAGCCATCAAACAAGTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCa                                                                                                                                               <  2:1178683/148‑1 (MQ=255)
    cGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTa                                                                                                                                            >  2:1989461/1‑148 (MQ=255)
      ccAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAt                                                                                                                                          >  1:62676/1‑148 (MQ=255)
      ccAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAt                                                                                                                                          <  1:1795449/148‑1 (MQ=255)
        aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                                                        >  2:2791679/1‑148 (MQ=255)
        aGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAttt                                                                                                                                        >  1:1621901/1‑148 (MQ=255)
          tggtgAGGGTAGCCATCAAACAATTCCTCTGAAATGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtg                                                                                                                                      >  2:158913/1‑148 (MQ=255)
          tggtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCGGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTCtg                                                                                                                                      <  2:3303397/148‑1 (MQ=255)
            gtgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTtgtg                                                                                                                                    >  2:1207601/1‑148 (MQ=255)
             tgAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGa                                                                                                                                   <  1:3264272/148‑1 (MQ=255)
                  gTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACtat                                                                                                                              >  1:1933417/1‑148 (MQ=255)
                       cATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcac                                                                                                                         <  1:2039739/148‑1 (MQ=255)
                        aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcaca                                                                                                                        <  1:1677004/148‑1 (MQ=255)
                        aTCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATcaca                                                                                                                        <  2:3268522/148‑1 (MQ=255)
                         tCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACACTGTTTGTATCATTAATTTTGTGAACTATATCACaa                                                                                                                       >  1:179716/1‑148 (MQ=255)
                           aaaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAatt                                                                                                                     >  1:3382568/1‑148 (MQ=255)
                            aaCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattg                                                                                                                    >  1:2557247/1‑148 (MQ=255)
                             aCAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattga                                                                                                                   >  2:1759148/1‑148 (MQ=255)
                              cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                                                  >  2:1536480/1‑148 (MQ=255)
                              cAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                                                  >  1:250379/1‑148 (MQ=255)
                              cAAGTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgat                                                                                                                  <  2:1341255/148‑1 (MQ=255)
                               aaTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgatt                                                                                                                 <  1:1490077/148‑1 (MQ=255)
                                aTTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAattgattg                                                                                                                <  1:2154886/148‑1 (MQ=255)
                                  tCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtt                                                                                                              >  1:1699880/1‑148 (MQ=255)
                                   ccTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgttt                                                                                                             >  2:228828/1‑148 (MQ=255)
                                    ctctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttg                                                                                                            >  2:3280001/1‑148 (MQ=255)
                                     tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                                           >  2:20483/1‑148 (MQ=255)
                                     tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                                           <  2:2676357/148‑1 (MQ=255)
                                     tctGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAttgtttgt                                                                                                           <  2:1637019/148‑1 (MQ=255)
                                         aaaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGc                                                                                                       >  1:2772931/1‑148 (MQ=255)
                                          aaaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGcc                                                                                                      >  2:480368/1‑148 (MQ=255)
                                           aaGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCa                                                                                                     >  2:3426454/1‑148 (MQ=255)
                                             gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                                                   <  1:354443/148‑1 (MQ=255)
                                             gagCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCata                                                                                                   >  1:2576190/1‑148 (MQ=255)
                                               gcggttGCCCTGGACTGGGCGGAGTTGTTCTCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAATATGAAACACTCTGTTTGTATCATTTATTTTGTGAACTATCTCACAATTGATTGTTTGTTAGCCATAtt                                                                                                 <  1:3180586/143‑1 (MQ=255)
                                                ccGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTa                                                                                                <  1:1155069/148‑1 (MQ=255)
                                                          ggACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACt                                                                                      >  2:1223003/1‑148 (MQ=255)
                                                           gACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtt                                                                                     >  2:1144421/1‑148 (MQ=255)
                                                            aCAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACttt                                                                                    <  2:3029761/148‑1 (MQ=255)
                                                             cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                                                   >  2:3107103/1‑148 (MQ=255)
                                                             cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                                                   <  2:2717667/148‑1 (MQ=255)
                                                             cAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACGATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACtttt                                                                                   <  2:1203316/148‑1 (MQ=255)
                                                                 gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTg                                                                               <  2:518420/148‑1 (MQ=255)
                                                                 gcggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAATTTTATTg                                                                               <  1:2514053/148‑1 (MQ=255)
                                                                  cggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGc                                                                              <  2:2770619/148‑1 (MQ=255)
                                                                   ggcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCt                                                                             <  1:1485266/148‑1 (MQ=255)
                                                                    gcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTg                                                                            <  1:2625009/148‑1 (MQ=255)
                                                                    gcgTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTGGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACGTTTATTGCTg                                                                            >  2:2976691/1‑148 (MQ=255)
                                                                       ttgttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTAc                                                                         <  2:1929210/148‑1 (MQ=255)
                                                                         gttCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAg                                                                       <  1:1758766/148‑1 (MQ=255)
                                                                           tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTATGCCGTGACTTTTATTTCTGTACAGag                                                                     >  2:3010843/1‑147 (MQ=255)
                                                                           tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAt                                                                     >  2:2632420/1‑148 (MQ=255)
                                                                           tCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAt                                                                     >  2:664065/1‑148 (MQ=255)
                                                                                ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgt                                                                >  2:2548904/1‑148 (MQ=255)
                                                                                ggAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgt                                                                <  2:2249283/148‑1 (MQ=255)
                                                                                 gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                                               <  2:1727864/148‑1 (MQ=255)
                                                                                 gAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTAtgtg                                                               >  1:298280/1‑148 (MQ=255)
                                                                                  aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                                              <  2:3372070/148‑1 (MQ=255)
                                                                                  aGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTgg                                                              <  2:418248/148‑1 (MQ=255)
                                                                                    gCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                                            <  1:2031975/148‑1 (MQ=255)
                                                                                    gCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGtt                                                            <  1:1668200/148‑1 (MQ=255)
                                                                                     cGTGGTACACTCTGGCTATCGCGGGGGTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGttt                                                           <  1:1089020/148‑1 (MQ=255)
                                                                                        ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                                        <  1:3079895/148‑1 (MQ=255)
                                                                                        ggTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTc                                                        <  1:2791679/148‑1 (MQ=255)
                                                                                          tACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAg                                                      >  1:1602202/1‑148 (MQ=255)
                                                                                               tctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCa                                                 <  1:946458/148‑1 (MQ=255)
                                                                                               tctGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCa                                                 >  1:1310602/1‑148 (MQ=255)
                                                                                                 tGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCCTATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCAtt                                               >  1:2386408/1‑148 (MQ=255)
                                                                                                  ggCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTa                                              >  1:1618793/1‑148 (MQ=255)
                                                                                                    cTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAg                                            <  1:1209415/148‑1 (MQ=255)
                                                                                                     tATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAgg                                           >  2:729465/1‑148 (MQ=255)
                                                                                                       tcacgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                         <  2:1469710/145‑1 (MQ=255)
                                                                                                       tCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGc                                         <  1:1191189/148‑1 (MQ=255)
                                                                                                         gcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCat                                       <  1:3411884/148‑1 (MQ=255)
                                                                                                         gcgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAGATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCat                                       >  1:2037790/1‑148 (MQ=255)
                                                                                                          cgGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCata                                      <  2:1133471/148‑1 (MQ=255)
                                                                                                             ggCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCt                                   >  1:797634/1‑148 (MQ=255)
                                                                                                              gCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCtt                                  >  2:1654587/1‑148 (MQ=255)
                                                                                                               cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc                                 <  2:508285/148‑1 (MQ=255)
                                                                                                               cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc                                 <  2:699524/148‑1 (MQ=255)
                                                                                                               cTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTc                                 <  1:2302819/148‑1 (MQ=255)
                                                                                                                   cAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCccgc                             >  1:488507/1‑148 (MQ=255)
                                                                                                                        aacaAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAGTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCct                        >  1:126620/2‑148 (MQ=255)
                                                                                                                         acaAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCctc                       >  1:288277/1‑148 (MQ=255)
                                                                                                                             aaaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCa                   <  2:3047071/148‑1 (MQ=255)
                                                                                                                             aaaTGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCa                   >  1:3034652/1‑148 (MQ=255)
                                                                                                                                tGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTc                >  1:2899361/1‑148 (MQ=255)
                                                                                                                                tGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTc                <  1:1021682/148‑1 (MQ=255)
                                                                                                                                    acacTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGt            >  2:2749177/1‑148 (MQ=255)
                                                                                                                                     cacTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTa           <  2:786917/148‑1 (MQ=255)
                                                                                                                                       ctctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAgg         >  1:1453072/1‑148 (MQ=255)
                                                                                                                                        tctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGa        >  2:1037374/1‑148 (MQ=255)
                                                                                                                                         ctGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGaa       >  1:2905621/1‑148 (MQ=255)
                                                                                                                                          tgtttgtaTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGaaa      <  1:2030827/148‑1 (MQ=255)
                                                                                                                                          tgtttgtaTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGaaa      <  1:862921/148‑1 (MQ=255)
                                                                                                                                            tttgtatCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaatt    >  2:2318603/1‑148 (MQ=255)
                                                                                                                                            tttgtatCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaatt    >  2:2082307/1‑148 (MQ=255)
                                                                                                                                             ttgtatCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAaatta   <  2:2160537/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAA  >  CP009273/4264944‑4265233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTT  >  CP009273/4264940‑4265236
                                                                                                                                                     |                                                                                                                                                   
AAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTA                                                                                                                                                     <  SRR19973065.2810821/150‑1 (MQ=60)
 AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTAT                                                                                                                                                    >  SRR19973065.1739319/1‑150 (MQ=60)
 AGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTAT                                                                                                                                                    >  SRR19973065.792133/1‑150 (MQ=60)
  GAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTTTC                                                                                                                                                   >  SRR19973065.1572489/1‑150 (MQ=60)
     CCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATT                                                                                                                                                <  SRR19973065.1289252/150‑1 (MQ=60)
        CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                                             <  SRR19973065.1191844/150‑1 (MQ=60)
        CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                                             <  SRR19973065.2952401/150‑1 (MQ=60)
        CGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAAT                                                                                                                                             >  SRR19973065.63346/1‑150 (MQ=60)
          CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                                           >  SRR19973065.695522/1‑150 (MQ=60)
          CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                                           <  SRR19973065.1808341/150‑1 (MQ=60)
          CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                                           >  SRR19973065.1632254/1‑150 (MQ=60)
          CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                                           <  SRR19973065.1807232/150‑1 (MQ=60)
          CCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTT                                                                                                                                           >  SRR19973065.1632773/1‑150 (MQ=60)
               GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGA                                                                                                                                      <  SRR19973065.2550220/150‑1 (MQ=60)
               GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGA                                                                                                                                      <  SRR19973065.1951353/150‑1 (MQ=60)
               GGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGA                                                                                                                                      <  SRR19973065.2546476/150‑1 (MQ=60)
                 TGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAAC                                                                                                                                    <  SRR19973065.3284059/150‑1 (MQ=60)
                   AGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTA                                                                                                                                  <  SRR19973065.1670053/150‑1 (MQ=60)
                    GGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTAT                                                                                                                                 >  SRR19973065.1945994/1‑150 (MQ=60)
                    GGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTAT                                                                                                                                 <  SRR19973065.39/150‑1 (MQ=60)
                    GGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTAT                                                                                                                                 >  SRR19973065.1946073/1‑150 (MQ=60)
                       TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                                              >  SRR19973065.2829467/1‑150 (MQ=60)
                       TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                                              >  SRR19973065.1067068/1‑150 (MQ=60)
                       TAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATC                                                                                                                              <  SRR19973065.897366/150‑1 (MQ=60)
                           CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAA                                                                                                                          <  SRR19973065.2052801/150‑1 (MQ=60)
                           CATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACACTGTTTGTATCATTAATTTTGTGAACTATATCACAA                                                                                                                          >  SRR19973065.181134/1‑150 (MQ=60)
                            ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                                         <  SRR19973065.1688976/150‑1 (MQ=60)
                            ATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAT                                                                                                                         <  SRR19973065.1688220/150‑1 (MQ=60)
                             TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                                        >  SRR19973065.3403250/1‑150 (MQ=60)
                             TCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATT                                                                                                                        <  SRR19973065.2152731/150‑1 (MQ=60)
                              CAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTG                                                                                                                       >  SRR19973065.2573239/1‑150 (MQ=60)
                                AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                                                     >  SRR19973065.252214/1‑150 (MQ=60)
                                AACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGAT                                                                                                                     >  SRR19973065.252135/1‑150 (MQ=60)
                                 ACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATT                                                                                                                    >  SRR19973065.3368718/1‑150 (MQ=60)
                                   AATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGT                                                                                                                  <  SRR19973065.1499340/150‑1 (MQ=60)
                                   AATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGT                                                                                                                  <  SRR19973065.1499629/150‑1 (MQ=60)
                                    ATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTT                                                                                                                 >  SRR19973065.1711214/1‑150 (MQ=60)
                                    ATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTT                                                                                                                 <  SRR19973065.2168745/150‑1 (MQ=60)
                                       CCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCACGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGT                                                                                                              >  SRR19973065.1479132/1‑150 (MQ=60)
                                           TGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGC                                                                                                          >  SRR19973065.2790154/1‑150 (MQ=60)
                                           TGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGC                                                                                                          >  SRR19973065.174317/1‑150 (MQ=60)
                                             AAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCA                                                                                                        <  SRR19973065.2301759/150‑1 (MQ=60)
                                               AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                      <  SRR19973065.2099192/150‑1 (MQ=60)
                                               AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                      <  SRR19973065.3080756/150‑1 (MQ=60)
                                               AAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATA                                                                                                      >  SRR19973065.2592258/1‑150 (MQ=60)
                                                 GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                                                    <  SRR19973065.356506/150‑1 (MQ=60)
                                                 GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                                                    <  SRR19973065.356938/150‑1 (MQ=60)
                                                 GAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATT                                                                                                    <  SRR19973065.1256155/150‑1 (MQ=60)
                                                   gcggtTGCCCTGGACTGGGCGGAGTTGTTCTCGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAATATGAAACACTCTGTTTGTATCATTTATTTTGTGAACTATCTCACAATTGATTGTTTGTTAGCCATATTAG                                                                                                  <  SRR19973065.3199806/145‑1 (MQ=60)
                                                   GCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAG                                                                                                  <  SRR19973065.3199988/150‑1 (MQ=60)
                                                    CCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGG                                                                                                 <  SRR19973065.1162491/150‑1 (MQ=60)
                                                        TGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGT                                                                                             <  SRR19973065.2805085/150‑1 (MQ=60)
                                                              GGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTTAACTATATCACAATTGATTGTTTGTTATCCATATTAGTCCGTTACTTT                                                                                       >  SRR19973065.3029275/1‑150 (MQ=60)
                                                                  AGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATT                                                                                   <  SRR19973065.3221647/150‑1 (MQ=60)
                                                                     GCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGAATTTTATTGCT                                                                                <  SRR19973065.2529849/150‑1 (MQ=60)
                                                                       GGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGT                                                                              <  SRR19973065.1494740/150‑1 (MQ=60)
                                                                       GGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGT                                                                              <  SRR19973065.1494759/150‑1 (MQ=60)
                                                                        GCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTA                                                                             <  SRR19973065.2641250/150‑1 (MQ=60)
                                                                        GCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTA                                                                             <  SRR19973065.2641242/150‑1 (MQ=60)
                                                                          GTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACA                                                                           >  SRR19973065.3107670/1‑150 (MQ=60)
                                                                             GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                                        <  SRR19973065.1770704/150‑1 (MQ=60)
                                                                             GTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAT                                                                        <  SRR19973065.1770321/150‑1 (MQ=60)
                                                                                  CGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGT                                                                   <  SRR19973065.205409/150‑1 (MQ=60)
                                                                                   GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                                                  >  SRR19973065.300207/1‑150 (MQ=60)
                                                                                   GGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTG                                                                  >  SRR19973065.299349/1‑150 (MQ=60)
                                                                                     GAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTCGGCCGTGACTTTTATTGCTGTACAGATTATGTGGT                                                                >  SRR19973065.436005/1‑150 (MQ=60)
                                                                                        GCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTT                                                             <  SRR19973065.2044983/150‑1 (MQ=60)
                                                                                        GCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTT                                                             <  SRR19973065.1679363/150‑1 (MQ=60)
                                                                                        GCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTT                                                             <  SRR19973065.2045016/150‑1 (MQ=60)
                                                                                         CGTGGTACACTCTGGCTATCGCGGGGGTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTT                                                            <  SRR19973065.1096197/150‑1 (MQ=60)
                                                                                            GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                         >  SRR19973065.1612912/1‑150 (MQ=60)
                                                                                            GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                         <  SRR19973065.2808985/150‑1 (MQ=60)
                                                                                            GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                         <  SRR19973065.2808880/150‑1 (MQ=60)
                                                                                            GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                         <  SRR19973065.1137201/150‑1 (MQ=60)
                                                                                            GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                         <  SRR19973065.3098706/150‑1 (MQ=60)
                                                                                            GGTACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAG                                                         <  SRR19973065.1340790/150‑1 (MQ=60)
                                                                                              TACACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTG                                                       >  SRR19973065.1684964/1‑150 (MQ=60)
                                                                                                 ACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCA                                                    <  SRR19973065.2718406/150‑1 (MQ=60)
                                                                                                 ACTCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCA                                                    >  SRR19973065.1319254/1‑150 (MQ=60)
                                                                                                   TCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCCTATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATT                                                  >  SRR19973065.2401377/1‑150 (MQ=60)
                                                                                                   TCTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATT                                                  <  SRR19973065.952714/150‑1 (MQ=60)
                                                                                                    CTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTA                                                 >  SRR19973065.1629281/1‑150 (MQ=60)
                                                                                                    CTGGCTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTA                                                 >  SRR19973065.1629651/1‑150 (MQ=60)
                                                                                                        CTATCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGG                                             <  SRR19973065.1217211/150‑1 (MQ=60)
                                                                                                           TCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCAT                                          <  SRR19973065.1198736/150‑1 (MQ=60)
                                                                                                           TCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCAT                                          >  SRR19973065.2050769/1‑150 (MQ=60)
                                                                                                           TCGCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCAGATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCAT                                          >  SRR19973065.2050848/1‑150 (MQ=60)
                                                                                                             GCGGGGCTTGCAGAAAACAAAAATTAAACACTCTGTTTTTATAATTAATTGTGTGAACTATATCTCAATTTATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATAT                                        <  SRR19973065.1986427/150‑1 (MQ=60)
                                                                                                             GCGGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATAT                                        <  SRR19973065.3432730/150‑1 (MQ=60)
                                                                                                               GGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCT                                      <  SRR19973065.836272/150‑1 (MQ=60)
                                                                                                               GGGGCTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCT                                      >  SRR19973065.802906/1‑150 (MQ=60)
                                                                                                                   CTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCC                                  <  SRR19973065.2317639/150‑1 (MQ=60)
                                                                                                                   CTTGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCC                                  <  SRR19973065.2317303/150‑1 (MQ=60)
                                                                                                                     TGCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGC                                >  SRR19973065.491809/1‑150 (MQ=60)
                                                                                                                      GCAGAAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCC                               <  SRR19973065.1206575/150‑1 (MQ=60)
                                                                                                                          AAAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGAGTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCT                           >  SRR19973065.127711/1‑150 (MQ=60)
                                                                                                                           AAACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTC                          >  SRR19973065.290161/1‑150 (MQ=60)
                                                                                                                            AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                         <  SRR19973065.3026357/150‑1 (MQ=60)
                                                                                                                            AACAAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCT                         <  SRR19973065.3413034/150‑1 (MQ=60)
                                                                                                                               AAAAATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCA                      >  SRR19973065.3053240/1‑150 (MQ=60)
                                                                                                                                  AATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTC                   >  SRR19973065.1630205/1‑150 (MQ=60)
                                                                                                                                  AATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTC                   >  SRR19973065.2917022/1‑150 (MQ=60)
                                                                                                                                  AATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTC                   >  SRR19973065.2917052/1‑150 (MQ=60)
                                                                                                                                  AATGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTC                   >  SRR19973065.2917059/1‑150 (MQ=60)
                                                                                                                                    TGAAACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCT                 <  SRR19973065.1028455/150‑1 (MQ=60)
                                                                                                                                       AACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTA              <  SRR19973065.160227/150‑1 (MQ=60)
                                                                                                                                       AACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTA              >  SRR19973065.1220480/1‑150 (MQ=60)
                                                                                                                                       AACACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAAATGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTACCCGCCGCCTCTGAAATCCTGTA              >  SRR19973065.510940/1‑150 (MQ=60)
                                                                                                                                         CACTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGG            >  SRR19973065.1462439/1‑150 (MQ=60)
                                                                                                                                           CTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAA          <  SRR19973065.1546614/150‑1 (MQ=60)
                                                                                                                                           CTCTGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAA          >  SRR19973065.2923331/1‑150 (MQ=60)
                                                                                                                                              TGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATT       <  SRR19973065.868473/150‑1 (MQ=60)
                                                                                                                                              TGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATT       <  SRR19973065.869529/150‑1 (MQ=60)
                                                                                                                                              TGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATT       <  SRR19973065.2043860/150‑1 (MQ=60)
                                                                                                                                              TGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATT       <  SRR19973065.258838/150‑1 (MQ=60)
                                                                                                                                              TGTTTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATT       <  SRR19973065.2498089/150‑1 (MQ=60)
                                                                                                                                                 TTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAAT    >  SRR19973065.2541493/1‑150 (MQ=60)
                                                                                                                                                 TTGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAAT    >  SRR19973065.2541485/1‑150 (MQ=60)
                                                                                                                                                  TGTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATT   <  SRR19973065.1714323/150‑1 (MQ=60)
                                                                                                                                                   GTATCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTT  <  SRR19973065.531533/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AAGAACCACGCCAGTGGTGAGGGTAGCCATCAAACAATTCCTCTGAAAAGAGCCGATGCCCTGGACAGCGCGGCGTTGTTCACGGGAGGCGTGGTACACTCTGGCTATCGCGGGGCTTGCAGAACACAAAAATGAAACACTCTGTTTGTTTCATTAATTTTGTGAACTATATCACAATTGATTGTTTGTTAGCCATATTAGGCCGTGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTT  >  CP009273/4264940‑4265236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑