Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 402,340 G→T 100% V160V (GTG→GTT)  aroL → shikimate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273402,3400GT99.5% 688.8 / ‑4.3 209V160V (GTG→GTT) aroLshikimate kinase II
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (102/106);  total (102/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

AAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA  >  CP009273/402195‑402484
                                                                                                                                                 |                                                                                                                                                
  gCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAttt                                                                                                                                              <  2:1253329/148‑1 (MQ=255)
   cTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTc                                                                                                                                             <  2:2409238/148‑1 (MQ=255)
       aCCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGaa                                                                                                                                         <  2:2757561/148‑1 (MQ=255)
        ccGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGaaa                                                                                                                                        <  1:581729/148‑1 (MQ=255)
          ggAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAtt                                                                                                                                      >  2:1537448/1‑148 (MQ=255)
               aagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAGATTCGCAg                                                                                                                                 >  1:856413/1‑148 (MQ=255)
                  aTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgcgc                                                                                                                              >  2:507234/1‑148 (MQ=255)
                    ttACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGccc                                                                                                                            <  1:61552/148‑1 (MQ=255)
                    ttACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGccc                                                                                                                            <  1:61582/148‑1 (MQ=255)
                      aCGGCCACCCTTAACGGGAAAACCGCTGAGCGAAGATGTTCAGGAAGTGCTGGAAGACCGCGTTGCGCTATAGCGCGAAGTTGCGCATATTATCATCGACGCTACAAACGAACCCAGCCAGGTTTTTTCTGATATTCGGAGCGCCCTg                                                                                                                          <  2:1588164/148‑1 (MQ=255)
                      aCGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTg                                                                                                                          >  2:3393707/1‑148 (MQ=255)
                          ccAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGcac                                                                                                                      >  2:1691534/1‑148 (MQ=255)
                             aCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGa                                                                                                                   >  2:1689151/1‑148 (MQ=255)
                             aCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGa                                                                                                                   >  1:2171232/1‑148 (MQ=255)
                             aCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGa                                                                                                                   <  2:551641/148‑1 (MQ=255)
                                ttAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAGATTCGCAGCGCCCTGGCACAGACGa                                                                                                                <  2:856413/148‑1 (MQ=255)
                                 tAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAt                                                                                                               >  1:1332544/1‑148 (MQ=255)
                                   aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             <  1:2160461/148‑1 (MQ=255)
                                      ggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAtt                                                                                                          >  1:920662/1‑148 (MQ=255)
                                      ggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAtt                                                                                                          >  1:1461358/1‑148 (MQ=255)
                                       gAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttg                                                                                                         >  1:3476579/1‑148 (MQ=255)
                                         aaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgtt                                                                                                       >  2:638416/1‑148 (MQ=255)
                                                 gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCg                                                                                               <  2:1128463/148‑1 (MQ=255)
                                                 gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCg                                                                                               <  2:632984/148‑1 (MQ=255)
                                                 gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCg                                                                                               >  2:2746378/1‑148 (MQ=255)
                                                  aGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTcta                                                                                              >  1:1364844/1‑146 (MQ=255)
                                                  aGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                              <  2:2293547/148‑1 (MQ=255)
                                                   gCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAg                                                                                             <  2:3142279/148‑1 (MQ=255)
                                                    cGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAGACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgc                                                                                            >  1:1056280/1‑148 (MQ=255)
                                                    cGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgc                                                                                            >  1:1785168/1‑148 (MQ=255)
                                                     gaagaaGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgcg                                                                                           <  1:3128872/148‑1 (MQ=255)
                                                      aagaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgcgc                                                                                          >  2:2777173/1‑148 (MQ=255)
                                                       agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGcc                                                                                         >  2:2175630/1‑148 (MQ=255)
                                                       agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGcc                                                                                         >  2:1603653/1‑148 (MQ=255)
                                                       agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGcc                                                                                         >  1:1038695/1‑148 (MQ=255)
                                                           gTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtata                                                                                     <  2:349745/148‑1 (MQ=255)
                                                            ttCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATAc                                                                                    >  2:1180040/1‑148 (MQ=255)
                                                              cAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACtt                                                                                  >  1:3077760/1‑148 (MQ=255)
                                                               aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 <  1:2487963/148‑1 (MQ=255)
                                                               aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 <  1:2317552/148‑1 (MQ=255)
                                                               aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 <  1:568662/148‑1 (MQ=255)
                                                               aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 <  1:524249/148‑1 (MQ=255)
                                                                ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGATCGCCTATACTTaa                                                                                >  1:195188/1‑148 (MQ=255)
                                                                ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTaa                                                                                >  1:274009/1‑148 (MQ=255)
                                                                   aGTGCTTGAAGAACGCGATGCGCTATATCGCGAAGTTTCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGt                                                                             >  2:748053/1‑148 (MQ=255)
                                                                    gTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGtt                                                                            <  2:3295679/148‑1 (MQ=255)
                                                                     tGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTc                                                                           <  1:2562101/148‑1 (MQ=255)
                                                                     tGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTc                                                                           <  1:2562526/148‑1 (MQ=255)
                                                                      gCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCa                                                                          <  1:2908872/148‑1 (MQ=255)
                                                                       cTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAt                                                                         <  1:1993087/148‑1 (MQ=255)
                                                                        tGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATc                                                                        <  2:20696/148‑1 (MQ=255)
                                                                          gaagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGGTCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATccc                                                                      >  1:362065/1‑148 (MQ=255)
                                                                          gaagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATccc                                                                      >  2:3207926/1‑148 (MQ=255)
                                                                          gaagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATccc                                                                      >  1:2454390/1‑148 (MQ=255)
                                                                           aagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                     >  1:2078947/1‑148 (MQ=255)
                                                                           aagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                     >  2:1111048/1‑148 (MQ=255)
                                                                            agaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGt                                                                    >  2:1101309/1‑148 (MQ=255)
                                                                            agaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGt                                                                    >  1:2835300/1‑148 (MQ=255)
                                                                            agaaCGCGAGGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGt                                                                    <  1:41150/148‑1 (MQ=255)
                                                                             gaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTg                                                                   >  1:2450562/1‑148 (MQ=255)
                                                                             gaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTg                                                                   >  1:911380/1‑148 (MQ=255)
                                                                                  ctaTGCGCTATATAGCTAATTTGCGAATATTTTCATCTACTCAACAAAAGAACCCAGCAAGTTTATTTCTTAAATTCGGTGTTCCCTGGCACAGAAGATAAATTGTTGATTTTCGTGAGCATATTCTTAACGTTCATCCCGTGAATTa                                                              <  2:2662959/146‑1 (MQ=255)
                                                                                  cgATGCGCTATATCGCGAAGTTGCGCATTTTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATa                                                              <  2:1788732/148‑1 (MQ=255)
                                                                                     tGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAgg                                                           <  1:3114136/148‑1 (MQ=255)
                                                                                         cTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaaga                                                       <  1:2175630/148‑1 (MQ=255)
                                                                                            tatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACg                                                    >  1:2397868/1‑148 (MQ=255)
                                                                                             atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGa                                                   >  1:1225330/1‑148 (MQ=255)
                                                                                               cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                 <  1:507234/148‑1 (MQ=255)
                                                                                                 cgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                               >  2:2259332/1‑148 (MQ=255)
                                                                                                      ttGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGa                                          >  2:2353402/1‑148 (MQ=255)
                                                                                                        gcgcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaaa                                        <  1:398075/148‑1 (MQ=255)
                                                                                                        gcgcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaaa                                        <  1:1537448/148‑1 (MQ=255)
                                                                                                            atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGc                                    >  2:1389228/1‑148 (MQ=255)
                                                                                                            atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGc                                    <  1:73157/148‑1 (MQ=255)
                                                                                                            atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGc                                    <  2:2031146/148‑1 (MQ=255)
                                                                                                                tatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTa                                >  2:768082/1‑148 (MQ=255)
                                                                                                                  tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATc                              <  2:3615/148‑1 (MQ=255)
                                                                                                                  tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATc                              <  1:3401895/148‑1 (MQ=255)
                                                                                                                  tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATc                              <  2:1785013/148‑1 (MQ=255)
                                                                                                                    atcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACTATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCt                            <  1:1590364/148‑1 (MQ=255)
                                                                                                                     tcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTc                           >  2:265196/1‑148 (MQ=255)
                                                                                                                        aCGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTg                        >  1:2100048/1‑148 (MQ=255)
                                                                                                                        aCGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTg                        >  1:285782/1‑148 (MQ=255)
                                                                                                                        aCGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTATCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCATATCCTCGTg                        >  1:3407582/1‑148 (MQ=255)
                                                                                                                             acaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCa                   <  2:231634/148‑1 (MQ=255)
                                                                                                                              caaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCat                  <  2:2485689/148‑1 (MQ=255)
                                                                                                                              caaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCat                  >  1:3130456/1‑148 (MQ=255)
                                                                                                                                aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatat                <  1:3345024/148‑1 (MQ=255)
                                                                                                                                aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatat                <  2:1463444/148‑1 (MQ=255)
                                                                                                                                aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatat                <  1:1733059/148‑1 (MQ=255)
                                                                                                                                 aCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatata               <  1:3082047/148‑1 (MQ=255)
                                                                                                                                  cGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat              <  2:2441745/148‑1 (MQ=255)
                                                                                                                                    aaCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAg            >  2:1815751/1‑148 (MQ=255)
                                                                                                                                      cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          >  2:2787179/1‑148 (MQ=255)
                                                                                                                                      cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          <  2:658502/148‑1 (MQ=255)
                                                                                                                                       ccagccagGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGa         <  2:2848876/148‑1 (MQ=255)
                                                                                                                                          gccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa      <  1:1180040/148‑1 (MQ=255)
                                                                                                                                          gccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa      >  2:2220044/1‑148 (MQ=255)
                                                                                                                                           ccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAt     <  2:1056280/148‑1 (MQ=255)
                                                                                                                                              ggTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGa  <  2:301523/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA  >  CP009273/402195‑402484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  >  CP009273/402191‑402489
                                                                                                                                                     |                                                                                                                                                     
CTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTT                                                                                                                                                       >  SRR19973066.1801481/1‑150 (MQ=60)
            CCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATT                                                                                                                                           <  SRR19973066.585802/150‑1 (MQ=60)
                 AGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAGATTCGCAG                                                                                                                                      >  SRR19973066.862591/1‑150 (MQ=60)
                        TTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTG                                                                                                                               <  SRR19973066.62345/150‑1 (MQ=60)
                        TTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTG                                                                                                                               <  SRR19973066.62327/150‑1 (MQ=60)
                        TTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTG                                                                                                                               <  SRR19973066.62315/150‑1 (MQ=60)
                        TTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTG                                                                                                                               >  SRR19973066.1108470/1‑150 (MQ=60)
                         TACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCACCAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGG                                                                                                                              <  SRR19973066.2149145/150‑1 (MQ=60)
                               CAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGA                                                                                                                        >  SRR19973066.2186309/1‑150 (MQ=60)
                               CAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGA                                                                                                                        >  SRR19973066.601873/1‑150 (MQ=60)
                                AACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAC                                                                                                                       >  SRR19973066.1194823/1‑150 (MQ=60)
                                AACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAC                                                                                                                       >  SRR19973066.1816718/1‑150 (MQ=60)
                                   CTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAT                                                                                                                    >  SRR19973066.1342217/1‑150 (MQ=60)
                                       ACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAT                                                                                                                <  SRR19973066.2175469/150‑1 (MQ=60)
                                        CGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATT                                                                                                               >  SRR19973066.927286/1‑150 (MQ=60)
                                        CGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATT                                                                                                               >  SRR19973066.1471695/1‑150 (MQ=60)
                                         GGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTG                                                                                                              >  SRR19973066.2720388/1‑150 (MQ=60)
                                         GGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTG                                                                                                              >  SRR19973066.3499786/1‑150 (MQ=60)
                                         GGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTG                                                                                                              >  SRR19973066.3499785/1‑150 (MQ=60)
                                          GGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGT                                                                                                             >  SRR19973066.2189714/1‑150 (MQ=60)
                                              AACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAT                                                                                                         >  SRR19973066.845429/1‑150 (MQ=60)
                                              AACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAT                                                                                                         <  SRR19973066.91361/150‑1 (MQ=60)
                                                    TGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCTA                                                                                                   >  SRR19973066.1374715/1‑150 (MQ=60)
                                                    TGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGA                                                                                                   >  SRR19973066.2545875/1‑150 (MQ=60)
                                                     GAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAG                                                                                                  >  SRR19973066.663309/1‑150 (MQ=60)
                                                      AGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAGACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGC                                                                                                 >  SRR19973066.1064043/1‑150 (MQ=60)
                                                      AGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGC                                                                                                 >  SRR19973066.1797748/1‑150 (MQ=60)
                                                      AGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGC                                                                                                 >  SRR19973066.1797904/1‑150 (MQ=60)
                                                         GAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCC                                                                                              >  SRR19973066.1046342/1‑150 (MQ=60)
                                                         GAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCC                                                                                              <  SRR19973066.3149760/150‑1 (MQ=60)
                                                             AAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATA                                                                                          >  SRR19973066.2463960/1‑150 (MQ=60)
                                                               GTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACT                                                                                        >  SRR19973066.383143/1‑150 (MQ=60)
                                                               GTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACT                                                                                        <  SRR19973066.405216/150‑1 (MQ=60)
                                                                TTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTT                                                                                       >  SRR19973066.3098408/1‑150 (MQ=60)
                                                                TTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTT                                                                                       >  SRR19973066.3098319/1‑150 (MQ=60)
                                                                TTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTT                                                                                       >  SRR19973066.1312377/1‑150 (MQ=60)
                                                                  CAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGATCGCCTATACTTAA                                                                                     >  SRR19973066.196863/1‑150 (MQ=60)
                                                                  CAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAA                                                                                     >  SRR19973066.276023/1‑150 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.572685/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.2504942/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.2504940/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.2333399/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.527543/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.1988119/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.572718/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    <  SRR19973066.528094/150‑1 (MQ=60)
                                                                     GAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGT                                                                                  <  SRR19973066.428706/150‑1 (MQ=60)
                                                                       AGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTC                                                                                <  SRR19973066.2372707/150‑1 (MQ=60)
                                                                         TGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAT                                                                              <  SRR19973066.2579920/150‑1 (MQ=60)
                                                                         TGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAT                                                                              >  SRR19973066.2001328/1‑150 (MQ=60)
                                                                         TGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAT                                                                              <  SRR19973066.2579913/150‑1 (MQ=60)
                                                                         TGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAT                                                                              <  SRR19973066.2579493/150‑1 (MQ=60)
                                                                          GCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATC                                                                             <  SRR19973066.2928290/150‑1 (MQ=60)
                                                                           CTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCC                                                                            <  SRR19973066.2007111/150‑1 (MQ=60)
                                                                            TGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGGTCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCC                                                                           >  SRR19973066.364901/1‑150 (MQ=60)
                                                                            TGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCC                                                                           >  SRR19973066.256185/1‑150 (MQ=60)
                                                                            TGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCC                                                                           >  SRR19973066.2471111/1‑150 (MQ=60)
                                                                            TGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCC                                                                           >  SRR19973066.2471006/1‑150 (MQ=60)
                                                                            TGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCC                                                                           >  SRR19973066.537496/1‑150 (MQ=60)
                                                                             GGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCG                                                                          >  SRR19973066.2093310/1‑150 (MQ=60)
                                                                             GGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCG                                                                          >  SRR19973066.2093308/1‑150 (MQ=60)
                                                                              GAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGT                                                                         >  SRR19973066.2854355/1‑150 (MQ=60)
                                                                               AAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTG                                                                        >  SRR19973066.1473785/1‑150 (MQ=60)
                                                                               AAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTG                                                                        >  SRR19973066.2467263/1‑150 (MQ=60)
                                                                               AAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTG                                                                        >  SRR19973066.917855/1‑150 (MQ=60)
                                                                                AGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                       >  SRR19973066.3308892/1‑150 (MQ=60)
                                                                                AGAACGCGAGGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                       <  SRR19973066.41700/150‑1 (MQ=60)
                                                                                    CGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATA                                                                   >  SRR19973066.1691379/1‑150 (MQ=60)
                                                                                      CGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAG                                                                 >  SRR19973066.977273/1‑150 (MQ=60)
                                                                                         TGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAA                                                              <  SRR19973066.3134403/150‑1 (MQ=60)
                                                                                         TGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAA                                                              <  SRR19973066.1455353/150‑1 (MQ=60)
                                                                                         TGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAA                                                              <  SRR19973066.3134935/150‑1 (MQ=60)
                                                                                             CTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAAC                                                          <  SRR19973066.2190792/150‑1 (MQ=60)
                                                                                             CTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAAC                                                          >  SRR19973066.105207/1‑150 (MQ=60)
                                                                                             CTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAAC                                                          <  SRR19973066.2190740/150‑1 (MQ=60)
                                                                                              TATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACG                                                         >  SRR19973066.2414036/1‑150 (MQ=60)
                                                                                               ATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGA                                                        >  SRR19973066.1234006/1‑150 (MQ=60)
                                                                                                   CGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCC                                                    <  SRR19973066.510998/150‑1 (MQ=60)
                                                                                                         GTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAA                                              >  SRR19973066.599039/1‑150 (MQ=60)
                                                                                                           TGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAAC                                            <  SRR19973066.3416352/150‑1 (MQ=60)
                                                                                                            GCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACC                                           <  SRR19973066.1548419/150‑1 (MQ=60)
                                                                                                            GCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACC                                           <  SRR19973066.401210/150‑1 (MQ=60)
                                                                                                                ATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCT                                       <  SRR19973066.74025/150‑1 (MQ=60)
                                                                                                                 TATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTT                                      >  SRR19973066.2049587/1‑150 (MQ=60)
                                                                                                                   TTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAGGGAAGAACGATGCCAACGAAACCGCCTTAT                                    <  SRR19973066.2997276/150‑1 (MQ=60)
                                                                                                                    TATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATC                                   <  SRR19973066.2797793/150‑1 (MQ=60)
                                                                                                                     ATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCC                                  >  SRR19973066.346422/1‑150 (MQ=60)
                                                                                                                      TCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCT                                 >  SRR19973066.1561599/1‑150 (MQ=60)
                                                                                                                      TCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCT                                 <  SRR19973066.3424606/150‑1 (MQ=60)
                                                                                                                      TCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCT                                 >  SRR19973066.924927/1‑150 (MQ=60)
                                                                                                                        ATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACTATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCG                               <  SRR19973066.1601870/150‑1 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                             >  SRR19973066.287750/1‑150 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                             >  SRR19973066.287846/1‑150 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                             >  SRR19973066.3430341/1‑150 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                             >  SRR19973066.2114504/1‑150 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTATCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCATATCCTCGTG                             >  SRR19973066.3430346/1‑150 (MQ=60)
                                                                                                                               CAACAAACGAACCCAGCCAGGTTATTTCTGAAAGTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCA                        <  SRR19973066.3347125/150‑1 (MQ=60)
                                                                                                                                AACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCAT                       >  SRR19973066.3151352/1‑150 (MQ=60)
                                                                                                                                AACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCAT                       >  SRR19973066.3151234/1‑150 (MQ=60)
                                                                                                                                 ACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATA                      <  SRR19973066.1362908/150‑1 (MQ=60)
                                                                                                                                   AAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATA                    <  SRR19973066.2179704/150‑1 (MQ=60)
                                                                                                                                    AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                   <  SRR19973066.1745575/150‑1 (MQ=60)
                                                                                                                                    AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                   <  SRR19973066.3367294/150‑1 (MQ=60)
                                                                                                                                     ACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATA                  <  SRR19973066.3102633/150‑1 (MQ=60)
                                                                                                                                     ACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATA                  <  SRR19973066.2922463/150‑1 (MQ=60)
                                                                                                                                        AACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTG               >  SRR19973066.3143420/1‑150 (MQ=60)
                                                                                                                                              GCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATT         <  SRR19973066.1188339/150‑1 (MQ=60)
                                                                                                                                                 AGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAA      >  SRR19973066.3313332/1‑150 (MQ=60)
                                                                                                                                                   GTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA    >  SRR19973066.1060116/1‑150 (MQ=60)
                                                                                                                                                     TATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  >  SRR19973066.1593792/1‑150 (MQ=60)
                                                                                                                                                     TATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  <  SRR19973066.1199165/150‑1 (MQ=60)
                                                                                                                                                     TATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTAAACGTTCATCCCGAGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTAGTGAAGCATATATAGTGACGATTGAAAAAG  >  SRR19973066.1634166/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  >  CP009273/402191‑402489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑