Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 402,340 G→T 100% V160V (GTG→GTT)  aroL → shikimate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273402,3400GT99.2% 837.6 / ‑2.3 253V160V (GTG→GTT) aroLshikimate kinase II
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (118/133);  total (119/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 253 total aligned reads displayed.

GCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAA  >  CP009273/402193‑402486
                                                                                                                                                   |                                                                                                                                                  
gCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAt                                                                                                                                                  <  1:3350827/150‑1 (MQ=255)
gCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAt                                                                                                                                                  <  2:217022/150‑1 (MQ=255)
 cAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAtt                                                                                                                                                 >  2:452289/1‑150 (MQ=255)
   aGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTc                                                                                                                                               >  2:2791043/1‑150 (MQ=255)
     cTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTg                                                                                                                                             <  2:5162895/150‑1 (MQ=255)
      tGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGa                                                                                                                                            >  1:1724207/1‑150 (MQ=255)
       gCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGaa                                                                                                                                           >  2:2990002/1‑150 (MQ=255)
          ccGGAAGAAGATTTACGGCCAATCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAtt                                                                                                                                        <  2:4833137/150‑1 (MQ=255)
             gaagaaGATTTACGTCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGc                                                                                                                                     >  2:3983635/1‑150 (MQ=255)
              aagaagATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCa                                                                                                                                    <  2:2109152/150‑1 (MQ=255)
                 aagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgcg                                                                                                                                 <  2:4382582/150‑1 (MQ=255)
                  agaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgcgc                                                                                                                                >  1:1699243/1‑150 (MQ=255)
                  agaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgcgc                                                                                                                                <  2:2540820/150‑1 (MQ=255)
                   gaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGcc                                                                                                                               >  1:2157108/1‑150 (MQ=255)
                   gaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGcc                                                                                                                               <  1:1058297/150‑1 (MQ=255)
                        aCGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGc                                                                                                                          >  1:1630712/1‑150 (MQ=255)
                        aCGGACAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGc                                                                                                                          <  2:3648990/150‑1 (MQ=255)
                         cGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGca                                                                                                                         <  1:2215739/150‑1 (MQ=255)
                         cGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGca                                                                                                                         >  1:539178/1‑150 (MQ=255)
                           gCCAACCGTAACGGGAAAACCGCTGAGCGAAGACGTTCAGGAAGTGCTGGAAGCACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGcaca                                                                                                                       <  2:4404638/150‑1 (MQ=255)
                                 cTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAt                                                                                                                 <  1:273296/150‑1 (MQ=255)
                                 cTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAt                                                                                                                 >  1:1150769/1‑150 (MQ=255)
                                 cTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAt                                                                                                                 >  1:3683818/1‑150 (MQ=255)
                                     aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAt                                                                                                             <  2:4307998/150‑1 (MQ=255)
                                     aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAt                                                                                                             <  2:1730955/150‑1 (MQ=255)
                                           aaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGa                                                                                                       >  1:4099837/1‑150 (MQ=255)
                                           aaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGa                                                                                                       >  2:1336965/1‑150 (MQ=255)
                                              ccGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAttt                                                                                                    <  2:2417897/150‑1 (MQ=255)
                                               cGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAtttt                                                                                                   <  1:433082/150‑1 (MQ=255)
                                                gCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTc                                                                                                  <  1:3044382/150‑1 (MQ=255)
                                                 cTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTNg                                                                                                 <  1:4040219/150‑1 (MQ=255)
                                                 cTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCg                                                                                                 <  1:4469714/150‑1 (MQ=255)
                                                  tGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                                >  1:3416530/1‑150 (MQ=255)
                                                   gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAg                                                                                               >  1:2620366/1‑150 (MQ=255)
                                                      cGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgcgc                                                                                            >  1:2510431/1‑150 (MQ=255)
                                                      cGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgcgc                                                                                            >  2:2844333/1‑150 (MQ=255)
                                                      cGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgcgc                                                                                            >  2:1950969/1‑150 (MQ=255)
                                                       gaagaaGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGcc                                                                                           >  1:1763593/1‑150 (MQ=255)
                                                        aagaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCt                                                                                          >  2:4627542/1‑150 (MQ=255)
                                                        aagaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCt                                                                                          >  1:1043323/1‑150 (MQ=255)
                                                          gaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtat                                                                                        <  1:690428/150‑1 (MQ=255)
                                                           aagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtata                                                                                       <  1:1113441/150‑1 (MQ=255)
                                                           aagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtata                                                                                       <  1:5028294/150‑1 (MQ=255)
                                                              ttCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACTCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACtt                                                                                    <  1:4352457/150‑1 (MQ=255)
                                                                 aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCCGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAc                                                                                 >  1:3067278/1‑150 (MQ=255)
                                                                   gAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGt                                                                               >  2:2177772/1‑150 (MQ=255)
                                                                    aaGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGtt                                                                              >  2:892738/1‑150 (MQ=255)
                                                                      gTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCa                                                                            >  2:3416912/1‑150 (MQ=255)
                                                                         cTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATcc                                                                         >  1:4286587/1‑150 (MQ=255)
                                                                          tGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATccc                                                                        >  1:2363962/1‑150 (MQ=255)
                                                                           ggAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                       >  2:743141/1‑150 (MQ=255)
                                                                                  cgcgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATa                                                                <  2:3767368/150‑1 (MQ=255)
                                                                                   gcgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATaa                                                               >  2:1039461/1‑150 (MQ=255)
                                                                                     gATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAgg                                                             <  1:1340434/150‑1 (MQ=255)
                                                                                        gcgcTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaag                                                          >  1:498235/1‑150 (MQ=255)
                                                                                           cTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAAc                                                       <  2:1519973/150‑1 (MQ=255)
                                                                                               atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                   <  2:2147031/150‑1 (MQ=255)
                                                                                               atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                   <  1:3897238/150‑1 (MQ=255)
                                                                                                 cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                                 >  2:61885/1‑150 (MQ=255)
                                                                                                 cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                                 >  1:4072028/1‑150 (MQ=255)
                                                                                                 cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                                 >  2:3540725/1‑150 (MQ=255)
                                                                                                 cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                                 <  1:886602/150‑1 (MQ=255)
                                                                                                 cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGcc                                                 <  2:4114791/150‑1 (MQ=255)
                                                                                                  gcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCa                                                <  1:4505311/150‑1 (MQ=255)
                                                                                                  gcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCa                                                <  1:3939640/150‑1 (MQ=255)
                                                                                                  gcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCa                                                <  1:234816/150‑1 (MQ=255)
                                                                                                    gAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAAc                                              >  1:782170/1‑150 (MQ=255)
                                                                                                     aaGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACg                                             >  2:2274117/1‑150 (MQ=255)
                                                                                                      aGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGGGAAATAAGGAAGAACGATGCCAACGa                                            >  1:293205/1‑150 (MQ=255)
                                                                                                        ttGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaaa                                          <  2:1612593/150‑1 (MQ=255)
                                                                                                           cgcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCg                                       <  1:3693459/150‑1 (MQ=255)
                                                                                                               tattatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCtt                                   <  1:2035687/150‑1 (MQ=255)
                                                                                                               tattatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCtt                                   <  1:2834957/150‑1 (MQ=255)
                                                                                                               tattatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCtt                                   >  1:1604189/1‑150 (MQ=255)
                                                                                                                 ttatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAt                                 <  1:4943523/150‑1 (MQ=255)
                                                                                                                   atcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATcc                               >  2:2446845/1‑150 (MQ=255)
                                                                                                                    tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCt                              <  1:743141/150‑1 (MQ=255)
                                                                                                                     catcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTc                             >  2:4474262/1‑150 (MQ=255)
                                                                                                                     catcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTc                             <  2:3060053/150‑1 (MQ=255)
                                                                                                                       tcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGt                           <  2:2668209/150‑1 (MQ=255)
                                                                                                                         gACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGa                         >  2:3616100/1‑150 (MQ=255)
                                                                                                                         gACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGa                         >  1:3790376/1‑150 (MQ=255)
                                                                                                                           cGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAg                       <  2:1222839/150‑1 (MQ=255)
                                                                                                                              aacaaACGAACCCAGCCATTTTATTTCTGAAATTCGCAGCGCCCTGGAACAGACGATAAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCat                    <  2:2746477/150‑1 (MQ=255)
                                                                                                                               acaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCata                   <  2:335635/150‑1 (MQ=255)
                                                                                                                                caaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatat                  >  1:3225182/1‑150 (MQ=255)
                                                                                                                                  aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat                <  1:1497401/150‑1 (MQ=255)
                                                                                                                                  aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat                <  1:2019438/150‑1 (MQ=255)
                                                                                                                                   aCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCAtatata               <  2:1711225/150‑1 (MQ=255)
                                                                                                                                    cGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAg              <  1:3767945/150‑1 (MQ=255)
                                                                                                                                    cGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAg              >  2:3683003/1‑150 (MQ=255)
                                                                                                                                      aaCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg            >  2:3860819/1‑150 (MQ=255)
                                                                                                                                       aCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGa           >  1:3476068/1‑150 (MQ=255)
                                                                                                                                         ccagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACg         <  2:3039232/150‑1 (MQ=255)
                                                                                                                                           agccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAt       <  1:3976463/150‑1 (MQ=255)
                                                                                                                                            gccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACATACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAtt      <  2:4720177/150‑1 (MQ=255)
                                                                                                                                              cagGTTATTTCTGAAATTCGCAGCGCCCTTGCACAGACGATCAATTGTTGATTTTCGTGCGCCTATTATTAACGTTCATCCCGTGATATATGGATGAACGATGCCATCGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAttta    <  2:68523/150‑3 (MQ=255)
                                                                                                                                               agGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGaa   >  2:2686682/1‑150 (MQ=255)
                                                                                                                                               agGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGaa   >  1:1779603/1‑150 (MQ=255)
                                                                                                                                               agGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGaa   >  2:3283955/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAA  >  CP009273/402193‑402486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  >  CP009273/402191‑402489
                                                                                                                                                     |                                                                                                                                                     
CTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTT                                                                                                                                                       <  SRR19973063.2332508/150‑1 (MQ=60)
 TGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTA                                                                                                                                                      <  SRR19973063.2825445/150‑1 (MQ=60)
  GCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAT                                                                                                                                                     <  SRR19973063.3361498/150‑1 (MQ=60)
  GCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAT                                                                                                                                                     <  SRR19973063.2896330/150‑1 (MQ=60)
  GCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAT                                                                                                                                                     <  SRR19973063.3361334/150‑1 (MQ=60)
   CAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATT                                                                                                                                                    <  SRR19973063.1213916/150‑1 (MQ=60)
     AGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTC                                                                                                                                                  >  SRR19973063.336607/1‑150 (MQ=60)
        TGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGA                                                                                                                                               >  SRR19973063.1729463/1‑150 (MQ=60)
           ACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAT                                                                                                                                            >  SRR19973063.4411068/1‑150 (MQ=60)
             CGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTC                                                                                                                                          <  SRR19973063.2772862/150‑1 (MQ=60)
              GGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCG                                                                                                                                         <  SRR19973063.2828866/150‑1 (MQ=60)
                AAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCA                                                                                                                                       >  SRR19973063.4090385/1‑150 (MQ=60)
                 AGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGAAGCTCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTTAAATTCGCAG                                                                                                                                      >  SRR19973063.1735309/1‑150 (MQ=60)
                  GAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGC                                                                                                                                     >  SRR19973063.4841506/1‑150 (MQ=60)
                    AGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGC                                                                                                                                   >  SRR19973063.1704423/1‑150 (MQ=60)
                    AGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGC                                                                                                                                   >  SRR19973063.2030350/1‑150 (MQ=60)
                     GATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCC                                                                                                                                  <  SRR19973063.1061503/150‑1 (MQ=60)
                     GATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCC                                                                                                                                  >  SRR19973063.2163867/1‑150 (MQ=60)
                      ATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCC                                                                                                                                 <  SRR19973063.3459965/150‑1 (MQ=60)
                          ACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGC                                                                                                                             >  SRR19973063.1635691/1‑150 (MQ=60)
                           CGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCA                                                                                                                            <  SRR19973063.2222725/150‑1 (MQ=60)
                           CGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCA                                                                                                                            >  SRR19973063.540770/1‑150 (MQ=60)
                            GGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCAC                                                                                                                           <  SRR19973063.4523639/150‑1 (MQ=60)
                            GGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCAC                                                                                                                           >  SRR19973063.3230911/1‑150 (MQ=60)
                               CAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGA                                                                                                                        >  SRR19973063.1643679/1‑150 (MQ=60)
                               CAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGA                                                                                                                        <  SRR19973063.5126651/150‑1 (MQ=60)
                                AACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAC                                                                                                                       >  SRR19973063.3857557/1‑150 (MQ=60)
                                AACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAC                                                                                                                       <  SRR19973063.4352865/150‑1 (MQ=60)
                                  CCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGA                                                                                                                     <  SRR19973063.1579539/150‑1 (MQ=60)
                                   CTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAT                                                                                                                    >  SRR19973063.1154246/1‑150 (MQ=60)
                                   CTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAT                                                                                                                    <  SRR19973063.274109/150‑1 (MQ=60)
                                   CTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAT                                                                                                                    >  SRR19973063.3695609/1‑150 (MQ=60)
                                    TTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATC                                                                                                                   <  SRR19973063.411549/150‑1 (MQ=60)
                                       ACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAT                                                                                                                >  SRR19973063.3042519/1‑150 (MQ=60)
                                       ACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAT                                                                                                                <  SRR19973063.823182/150‑1 (MQ=60)
                                            AAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTA                                                                                                           <  SRR19973063.3750156/150‑1 (MQ=60)
                                             AAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGA                                                                                                          >  SRR19973063.4112974/1‑150 (MQ=60)
                                                 CGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTT                                                                                                      <  SRR19973063.434285/150‑1 (MQ=60)
                                                  GCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTC                                                                                                     >  SRR19973063.3560976/1‑150 (MQ=60)
                                                  GCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTC                                                                                                     <  SRR19973063.3054175/150‑1 (MQ=60)
                                                  GCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTC                                                                                                     <  SRR19973063.3054038/150‑1 (MQ=60)
                                                  GCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTC                                                                                                     <  SRR19973063.3054118/150‑1 (MQ=60)
                                                  GCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTC                                                                                                     <  SRR19973063.3935305/150‑1 (MQ=60)
                                                   CTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTNG                                                                                                    <  SRR19973063.4053169/150‑1 (MQ=60)
                                                   CTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCG                                                                                                    <  SRR19973063.4484050/150‑1 (MQ=60)
                                                    TGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGA                                                                                                   >  SRR19973063.3427406/1‑150 (MQ=60)
                                                     GAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAG                                                                                                  >  SRR19973063.2628711/1‑150 (MQ=60)
                                                        CGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGC                                                                                               >  SRR19973063.2261459/1‑150 (MQ=60)
                                                        CGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGC                                                                                               >  SRR19973063.2518434/1‑150 (MQ=60)
                                                         GAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCC                                                                                              >  SRR19973063.1769018/1‑150 (MQ=60)
                                                          AAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCT                                                                                             >  SRR19973063.1046484/1‑150 (MQ=60)
                                                           AGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTA                                                                                            >  SRR19973063.3993231/1‑150 (MQ=60)
                                                           AGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTA                                                                                            >  SRR19973063.161725/1‑150 (MQ=60)
                                                            GAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTAT                                                                                           <  SRR19973063.692459/150‑1 (MQ=60)
                                                             AAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATA                                                                                          <  SRR19973063.5044511/150‑1 (MQ=60)
                                                             AAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATA                                                                                          <  SRR19973063.1116830/150‑1 (MQ=60)
                                                             AAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATA                                                                                          <  SRR19973063.1116681/150‑1 (MQ=60)
                                                                TTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACTCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTT                                                                                       <  SRR19973063.4366361/150‑1 (MQ=60)
                                                                  CAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAA                                                                                     <  SRR19973063.2359828/150‑1 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCCGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    >  SRR19973063.3077131/1‑150 (MQ=60)
                                                                   AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                    >  SRR19973063.556627/1‑150 (MQ=60)
                                                                    GGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACG                                                                                   <  SRR19973063.5017870/150‑1 (MQ=60)
                                                                      AAGTGATGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACTAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTT                                                                                 <  SRR19973063.893652/150‑1 (MQ=60)
                                                                         TGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAT                                                                              <  SRR19973063.3519058/150‑1 (MQ=60)
                                                                           CTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCC                                                                            >  SRR19973063.4300206/1‑150 (MQ=60)
                                                                           CTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCC                                                                            >  SRR19973063.4371390/1‑150 (MQ=60)
                                                                            TGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCC                                                                           >  SRR19973063.2371537/1‑150 (MQ=60)
                                                                               AAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTG                                                                        <  SRR19973063.1589673/150‑1 (MQ=60)
                                                                                AGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                       >  SRR19973063.2101897/1‑150 (MQ=60)
                                                                                AGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                       >  SRR19973063.2101778/1‑150 (MQ=60)
                                                                                AGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                       <  SRR19973063.1391601/150‑1 (MQ=60)
                                                                                  AACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAA                                                                     >  SRR19973063.1056219/1‑150 (MQ=60)
                                                                                       GATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGG                                                                <  SRR19973063.2210665/150‑1 (MQ=60)
                                                                                       GATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGG                                                                <  SRR19973063.1344457/150‑1 (MQ=60)
                                                                                          GCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAG                                                             >  SRR19973063.499668/1‑150 (MQ=60)
                                                                                          GCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAG                                                             >  SRR19973063.2275322/1‑150 (MQ=60)
                                                                                           CGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGA                                                            <  SRR19973063.4001710/150‑1 (MQ=60)
                                                                                                 ATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATG                                                      <  SRR19973063.3909651/150‑1 (MQ=60)
                                                                                                 ATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATG                                                      <  SRR19973063.3892071/150‑1 (MQ=60)
                                                                                                   CGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCC                                                    >  SRR19973063.4084990/1‑150 (MQ=60)
                                                                                                   CGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCC                                                    <  SRR19973063.889165/150‑1 (MQ=60)
                                                                                                   CGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCC                                                    >  SRR19973063.4085077/1‑150 (MQ=60)
                                                                                                   CGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCC                                                    <  SRR19973063.2593087/150‑1 (MQ=60)
                                                                                                    GCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCA                                                   <  SRR19973063.4519772/150‑1 (MQ=60)
                                                                                                    GCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCA                                                   <  SRR19973063.235526/150‑1 (MQ=60)
                                                                                                    GCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCA                                                   <  SRR19973063.4519284/150‑1 (MQ=60)
                                                                                                    GCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCA                                                   <  SRR19973063.3952235/150‑1 (MQ=60)
                                                                                                      GAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAAC                                                 >  SRR19973063.784438/1‑150 (MQ=60)
                                                                                                        AGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGA                                               >  SRR19973063.1982155/1‑150 (MQ=60)
                                                                                                        AGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGGGAAATAAGGAAGAACGATGCCAACGA                                               >  SRR19973063.294073/1‑150 (MQ=60)
                                                                                                          TTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAA                                             <  SRR19973063.5037835/150‑1 (MQ=60)
                                                                                                          TTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAA                                             >  SRR19973063.1714654/1‑150 (MQ=60)
                                                                                                          TTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAA                                             >  SRR19973063.4300741/1‑150 (MQ=60)
                                                                                                            GCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACC                                           <  SRR19973063.679106/150‑1 (MQ=60)
                                                                                                             CGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCG                                          <  SRR19973063.3705284/150‑1 (MQ=60)
                                                                                                             CGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCG                                          <  SRR19973063.1116452/150‑1 (MQ=60)
                                                                                                               CATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCC                                        >  SRR19973063.770672/1‑150 (MQ=60)
                                                                                                               CATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCC                                        >  SRR19973063.770676/1‑150 (MQ=60)
                                                                                                                 TATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTT                                      <  SRR19973063.2042049/150‑1 (MQ=60)
                                                                                                                 TATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTT                                      >  SRR19973063.1609101/1‑150 (MQ=60)
                                                                                                                 TATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTT                                      <  SRR19973063.2844059/150‑1 (MQ=60)
                                                                                                                 TATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTT                                      <  SRR19973063.3698223/150‑1 (MQ=60)
                                                                                                                   TTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAT                                    <  SRR19973063.24758/150‑1 (MQ=60)
                                                                                                                   TTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAT                                    <  SRR19973063.4959554/150‑1 (MQ=60)
                                                                                                                   TTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAT                                    <  SRR19973063.4959513/150‑1 (MQ=60)
                                                                                                                   TTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAT                                    <  SRR19973063.1737813/150‑1 (MQ=60)
                                                                                                                    TATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATC                                   <  SRR19973063.3769523/150‑1 (MQ=60)
                                                                                                                      TCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCT                                 <  SRR19973063.745318/150‑1 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                             >  SRR19973063.1149214/1‑150 (MQ=60)
                                                                                                                          CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                             <  SRR19973063.1642697/150‑1 (MQ=60)
                                                                                                                           GACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGA                            >  SRR19973063.3802465/1‑150 (MQ=60)
                                                                                                                               CAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGAA                        <  SRR19973063.693303/150‑1 (MQ=60)
                                                                                                                                AACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCAT                       >  SRR19973063.3910752/1‑150 (MQ=60)
                                                                                                                                 ACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATA                      >  SRR19973063.3216942/1‑150 (MQ=60)
                                                                                                                                  CAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATAT                     >  SRR19973063.3235544/1‑150 (MQ=60)
                                                                                                                                    AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                   <  SRR19973063.2025740/150‑1 (MQ=60)
                                                                                                                                    AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                   <  SRR19973063.1501986/150‑1 (MQ=60)
                                                                                                                                     ACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATA                  >  SRR19973063.4314624/1‑150 (MQ=60)
                                                                                                                                     ACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATA                  <  SRR19973063.1279735/150‑1 (MQ=60)
                                                                                                                                      CGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAG                 <  SRR19973063.3779982/150‑1 (MQ=60)
                                                                                                                                      CGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAG                 <  SRR19973063.4088847/150‑1 (MQ=60)
                                                                                                                                       GAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGT                >  SRR19973063.3786838/1‑150 (MQ=60)
                                                                                                                                         ACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGA              >  SRR19973063.3487152/1‑150 (MQ=60)
                                                                                                                                         ACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGA              >  SRR19973063.2652291/1‑150 (MQ=60)
                                                                                                                                           CCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACG            <  SRR19973063.2581755/150‑1 (MQ=60)
                                                                                                                                             AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT          <  SRR19973063.3989204/150‑1 (MQ=60)
                                                                                                                                             AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT          <  SRR19973063.2366385/150‑1 (MQ=60)
                                                                                                                                             AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT          <  SRR19973063.3989194/150‑1 (MQ=60)
                                                                                                                                              GCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATT         <  SRR19973063.3412496/150‑1 (MQ=60)
                                                                                                                                                 AGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAA      >  SRR19973063.1782475/1‑150 (MQ=60)
                                                                                                                                                 AGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAA      >  SRR19973063.1785095/1‑150 (MQ=60)
                                                                                                                                                 AGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAA      >  SRR19973063.1926008/1‑150 (MQ=60)
                                                                                                                                                  GGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAA     <  SRR19973063.854279/150‑1 (MQ=60)
                                                                                                                                                   GTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA    >  SRR19973063.4648066/1‑150 (MQ=60)
                                                                                                                                                   GTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA    >  SRR19973063.4644565/1‑150 (MQ=60)
                                                                                                                                                     TATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  >  SRR19973063.4718078/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAG  >  CP009273/402191‑402489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑