Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 402,340 G→T 100% V160V (GTG→GTT)  aroL → shikimate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273402,3400GT99.1% 794.8 / ‑1.5 223V160V (GTG→GTT) aroLshikimate kinase II
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (107/114);  total (107/116)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

CAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA  >  CP009273/402194‑402484
                                                                                                                                                  |                                                                                                                                                
cAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACTGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGAtt                                                                                                                                               <  2:2119856/150‑1 (MQ=255)
cAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAtt                                                                                                                                               <  1:1179288/150‑1 (MQ=255)
 aaGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAttt                                                                                                                                              <  1:3842842/150‑1 (MQ=255)
  aGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTc                                                                                                                                             >  2:3653065/1‑150 (MQ=255)
  aGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTc                                                                                                                                             >  1:2175166/1‑150 (MQ=255)
   gCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCt                                                                                                                                            <  1:3490705/150‑1 (MQ=255)
      gccccGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGaa                                                                                                                                         >  1:522667/4‑150 (MQ=255)
         ccGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAtt                                                                                                                                      <  1:2758591/150‑1 (MQ=255)
         ccGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAtt                                                                                                                                      <  1:358394/150‑1 (MQ=255)
          cGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTc                                                                                                                                     <  1:685336/150‑1 (MQ=255)
             aagttgaTTTTCGGCCATACTTAACGGGAAAACCGCTGAGCGAAGACGTTCTGGAAGTGCTGGAAGAACGCGATGCGCTATTTCGCGAAGTTGCGCATATTATCATCGACGCAACTACCGAACCCAGCCAGGTTATTTCTGAAATTCGCa                                                                                                                                  <  1:574518/145‑1 (MQ=255)
             aagaagATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCa                                                                                                                                  <  1:2950803/150‑1 (MQ=255)
                   aTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGccc                                                                                                                            >  2:389266/1‑150 (MQ=255)
                    tttACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCt                                                                                                                           <  2:539150/150‑1 (MQ=255)
                    tttACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCt                                                                                                                           <  1:715444/150‑1 (MQ=255)
                        cGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGca                                                                                                                       <  1:3760954/150‑1 (MQ=255)
                          gCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGcaca                                                                                                                     <  2:2250093/150‑1 (MQ=255)
                           ccAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAg                                                                                                                    <  1:2808395/150‑1 (MQ=255)
                            cAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGa                                                                                                                   >  1:2892281/1‑150 (MQ=255)
                             aaCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAc                                                                                                                  <  1:4045972/150‑1 (MQ=255)
                             aaCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAc                                                                                                                  <  2:410365/150‑1 (MQ=255)
                              aCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACg                                                                                                                 >  2:650208/1‑150 (MQ=255)
                               ccTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGa                                                                                                                <  2:652603/150‑1 (MQ=255)
                                cTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAt                                                                                                               <  1:2593001/150‑1 (MQ=255)
                                 ttAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATc                                                                                                              >  1:2447431/1‑150 (MQ=255)
                                 ttAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATc                                                                                                              >  1:1494957/1‑150 (MQ=255)
                                  tAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             >  2:1393719/1‑150 (MQ=255)
                                    aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAt                                                                                                           <  2:3528843/150‑1 (MQ=255)
                                      gggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttg                                                                                                         >  2:1501203/1‑150 (MQ=255)
                                      gggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttg                                                                                                         <  2:1888987/150‑1 (MQ=255)
                                           aaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAt                                                                                                    >  2:2019000/1‑150 (MQ=255)
                                             ccGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAttt                                                                                                  <  2:1678645/150‑1 (MQ=255)
                                              cGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAtttt                                                                                                 <  2:1878168/150‑1 (MQ=255)
                                                  gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAg                                                                                             >  1:2432915/1‑150 (MQ=255)
                                                  gAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAATGTTGATTTTCGAg                                                                                             >  1:1948667/1‑150 (MQ=255)
                                                      gaagaaGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGcc                                                                                         >  2:3772629/1‑150 (MQ=255)
                                                      gaagaaGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGcc                                                                                         <  1:1968755/150‑1 (MQ=255)
                                                        agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                       <  2:1153193/150‑1 (MQ=255)
                                                        agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                       <  1:102122/150‑1 (MQ=255)
                                                        agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                       >  2:1875330/1‑150 (MQ=255)
                                                        agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                       >  1:114894/1‑150 (MQ=255)
                                                        agaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                       >  1:3218133/1‑150 (MQ=255)
                                                         gaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCtat                                                                                      <  2:844574/150‑1 (MQ=255)
                                                             ttCAGGAAGTGCTCGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACtt                                                                                  <  2:3354493/150‑1 (MQ=255)
                                                              tCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 <  1:2303828/150‑1 (MQ=255)
                                                                 ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACg                                                                              <  2:3122824/150‑1 (MQ=255)
                                                                     gTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCa                                                                          <  1:3635416/150‑1 (MQ=255)
                                                                          ggAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                     <  1:2508698/150‑1 (MQ=255)
                                                                          ggAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                     <  1:3006852/150‑1 (MQ=255)
                                                                            aagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTg                                                                   <  1:1260435/150‑1 (MQ=255)
                                                                             agaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGa                                                                  <  1:3968854/150‑1 (MQ=255)
                                                                               aaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGaaa                                                                <  1:1738135/150‑1 (MQ=255)
                                                                                 cgcgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATa                                                              <  2:2722601/150‑1 (MQ=255)
                                                                                  gcgATGCGCTATATCGCGAAGTTGCGCATTTTATCATCGACGCAACAAACGTACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATaa                                                             <  1:412912/150‑1 (MQ=255)
                                                                                   cgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAg                                                            <  1:3412295/150‑1 (MQ=255)
                                                                                   cgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAg                                                            >  1:1345816/1‑150 (MQ=255)
                                                                                    gATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAgg                                                           >  1:1836954/1‑150 (MQ=255)
                                                                                      tGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaa                                                         >  1:3675843/1‑150 (MQ=255)
                                                                                      tGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaa                                                         <  2:1185166/150‑1 (MQ=255)
                                                                                      tGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaa                                                         <  2:3989648/150‑1 (MQ=255)
                                                                                         gcTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaagaa                                                      <  2:2450755/150‑1 (MQ=255)
                                                                                              atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                 >  1:1664918/1‑150 (MQ=255)
                                                                                              atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                 <  2:1981656/150‑1 (MQ=255)
                                                                                                  cgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCaa                                             >  2:2547603/1‑150 (MQ=255)
                                                                                                  cgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCaa                                             >  1:169113/1‑150 (MQ=255)
                                                                                                  cgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCaa                                             >  1:1368443/1‑150 (MQ=255)
                                                                                                   gAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAAc                                            >  1:1884733/1‑150 (MQ=255)
                                                                                                    aaGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACg                                           >  2:3755602/1‑150 (MQ=255)
                                                                                                          cgcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCg                                     <  1:2875567/150‑1 (MQ=255)
                                                                                                            cATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGcc                                   <  2:1580929/150‑1 (MQ=255)
                                                                                                             atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCt                                  >  2:4170253/1‑150 (MQ=255)
                                                                                                             atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCt                                  >  2:2754013/1‑150 (MQ=255)
                                                                                                             atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCt                                  >  2:3226246/1‑150 (MQ=255)
                                                                                                              tattatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCtt                                 <  1:3088656/150‑1 (MQ=255)
                                                                                                                 tatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATc                              >  1:1008696/1‑150 (MQ=255)
                                                                                                                   tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCNt                            <  2:2638111/150‑1 (MQ=255)
                                                                                                                    catcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTc                           <  2:3500486/150‑1 (MQ=255)
                                                                                                                    catcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTc                           >  2:4148668/1‑150 (MQ=255)
                                                                                                                     atcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCg                          <  1:3312326/150‑1 (MQ=255)
                                                                                                                        gACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGa                       >  2:2904333/1‑150 (MQ=255)
                                                                                                                         aCGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGaa                      <  1:711501/150‑1 (MQ=255)
                                                                                                                         aCGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGaa                      <  1:394219/150‑1 (MQ=255)
                                                                                                                            caacaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCa                   <  1:803347/150‑1 (MQ=255)
                                                                                                                                aaaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatata               >  2:2935446/1‑150 (MQ=255)
                                                                                                                                aaaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatata               <  2:2178699/150‑1 (MQ=255)
                                                                                                                                 aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat              >  2:2955329/1‑150 (MQ=255)
                                                                                                                                     aaCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          <  1:3033330/150‑1 (MQ=255)
                                                                                                                                      aCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGa         >  2:3267744/1‑150 (MQ=255)
                                                                                                                                      aCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGa         <  1:974972/150‑1 (MQ=255)
                                                                                                                                       cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAc        >  1:3819205/1‑150 (MQ=255)
                                                                                                                                       cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAc        >  2:3095748/1‑150 (MQ=255)
                                                                                                                                       cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAc        >  1:1611701/1‑150 (MQ=255)
                                                                                                                                         cagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa      <  2:1758847/150‑1 (MQ=255)
                                                                                                                                         cagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa      >  2:956948/1‑150 (MQ=255)
                                                                                                                                          agccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAt     <  1:306394/150‑1 (MQ=255)
                                                                                                                                          agccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAt     <  1:2339566/150‑1 (MQ=255)
                                                                                                                                           gccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAtt    <  2:2461413/150‑1 (MQ=255)
                                                                                                                                           gccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAtt    <  2:339812/150‑1 (MQ=255)
                                                                                                                                            ccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTg   <  2:1242253/150‑1 (MQ=255)
                                                                                                                                             cagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGa  >  1:2845784/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA  >  CP009273/402194‑402484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA  >  CP009273/402192‑402487
                                                                                                                                                    |                                                                                                                                                   
TGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTA                                                                                                                                                    >  SRR19973074.508893/1‑150 (MQ=60)
  CAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATT                                                                                                                                                  <  SRR19973074.1181533/150‑1 (MQ=60)
   AAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTT                                                                                                                                                 <  SRR19973074.3849843/150‑1 (MQ=60)
   AAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTT                                                                                                                                                 <  SRR19973074.3849847/150‑1 (MQ=60)
   AAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTT                                                                                                                                                 >  SRR19973074.1112358/1‑150 (MQ=60)
    AGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTC                                                                                                                                                >  SRR19973074.2179251/1‑150 (MQ=60)
     GCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCT                                                                                                                                               <  SRR19973074.3497109/150‑1 (MQ=60)
     GCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCT                                                                                                                                               <  SRR19973074.3497058/150‑1 (MQ=60)
       TGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGA                                                                                                                                             >  SRR19973074.2882967/1‑150 (MQ=60)
        GCCCCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAA                                                                                                                                            >  SRR19973074.523709/1‑150 (MQ=60)
        GCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAA                                                                                                                                            >  SRR19973074.523710/1‑150 (MQ=60)
          ACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAAT                                                                                                                                          <  SRR19973074.1541904/150‑1 (MQ=60)
           CCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATT                                                                                                                                         <  SRR19973074.359053/150‑1 (MQ=60)
           CCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATT                                                                                                                                         <  SRR19973074.359051/150‑1 (MQ=60)
           CCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATT                                                                                                                                         <  SRR19973074.2763670/150‑1 (MQ=60)
            CGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTC                                                                                                                                        <  SRR19973074.686594/150‑1 (MQ=60)
            CGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTC                                                                                                                                        <  SRR19973074.3971329/150‑1 (MQ=60)
               aagttgattttcggccataCTTAACGGGAAAACCGCTGAGCGAAGACGTTCTGGAAGTGCTGGAAGAACGCGATGCGCTATTTCGCGAAGTTGCGCATATTATCATCGACGCAACTACCGAACCCAGCCAGGTTATTTCTGAAATTCGCA                                                                                                                                     <  SRR19973074.575639/131‑1 (MQ=60)
               AAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCA                                                                                                                                     <  SRR19973074.2956290/150‑1 (MQ=60)
               AAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCA                                                                                                                                     <  SRR19973074.575652/150‑1 (MQ=60)
                      TTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCT                                                                                                                              <  SRR19973074.1834965/150‑1 (MQ=60)
                      TTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCT                                                                                                                              <  SRR19973074.716747/150‑1 (MQ=60)
                      TTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCT                                                                                                                              <  SRR19973074.716777/150‑1 (MQ=60)
                       TTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTG                                                                                                                             <  SRR19973074.2260906/150‑1 (MQ=60)
                          CGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCA                                                                                                                          <  SRR19973074.3767865/150‑1 (MQ=60)
                             CCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAG                                                                                                                       <  SRR19973074.2813807/150‑1 (MQ=60)
                             CCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAG                                                                                                                       <  SRR19973074.2813581/150‑1 (MQ=60)
                              CAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGA                                                                                                                      >  SRR19973074.2897646/1‑150 (MQ=60)
                               AACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGAC                                                                                                                     <  SRR19973074.4053451/150‑1 (MQ=60)
                                  CTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAT                                                                                                                  <  SRR19973074.2597849/150‑1 (MQ=60)
                                   TTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATC                                                                                                                 >  SRR19973074.2451959/1‑150 (MQ=60)
                                   TTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATC                                                                                                                 >  SRR19973074.367045/1‑150 (MQ=60)
                                   TTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATC                                                                                                                 >  SRR19973074.1497777/1‑150 (MQ=60)
                                       CGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATT                                                                                                             <  SRR19973074.1696541/150‑1 (MQ=60)
                                        GGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTG                                                                                                            >  SRR19973074.2989914/1‑150 (MQ=60)
                                         GGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGT                                                                                                           >  SRR19973074.3295794/1‑150 (MQ=60)
                                                CGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTT                                                                                                    <  SRR19973074.3781072/150‑1 (MQ=60)
                                                    GAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAG                                                                                                >  SRR19973074.1952399/1‑150 (MQ=60)
                                                    GAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAG                                                                                                >  SRR19973074.2437415/1‑150 (MQ=60)
                                                    GAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAG                                                                                                >  SRR19973074.2437465/1‑150 (MQ=60)
                                                    GAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAATGTTGATTTTCGAG                                                                                                >  SRR19973074.1952388/1‑150 (MQ=60)
                                                        GAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCC                                                                                            <  SRR19973074.1972509/150‑1 (MQ=60)
                                                          AGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTA                                                                                          >  SRR19973074.3224079/1‑150 (MQ=60)
                                                          AGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTA                                                                                          >  SRR19973074.115168/1‑150 (MQ=60)
                                                          AGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTA                                                                                          <  SRR19973074.102372/150‑1 (MQ=60)
                                                           GAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTAT                                                                                         >  SRR19973074.3387406/1‑150 (MQ=60)
                                                            AAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATA                                                                                        >  SRR19973074.859960/1‑150 (MQ=60)
                                                                TCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTA                                                                                    <  SRR19973074.2308134/150‑1 (MQ=60)
                                                                 CAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAA                                                                                   >  SRR19973074.3065596/1‑150 (MQ=60)
                                                                  AGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAAC                                                                                  >  SRR19973074.2907720/1‑150 (MQ=60)
                                                                     AAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTT                                                                               <  SRR19973074.3557602/150‑1 (MQ=60)
                                                                      AGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTC                                                                              >  SRR19973074.121705/1‑150 (MQ=60)
                                                                       GTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCA                                                                             <  SRR19973074.3642138/150‑1 (MQ=60)
                                                                          CTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCC                                                                          <  SRR19973074.1623470/150‑1 (MQ=60)
                                                                          CTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCC                                                                          <  SRR19973074.1622954/150‑1 (MQ=60)
                                                                            GGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCG                                                                        <  SRR19973074.2513371/150‑1 (MQ=60)
                                                                            GGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCG                                                                        <  SRR19973074.3012457/150‑1 (MQ=60)
                                                                            GGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCG                                                                        <  SRR19973074.3012427/150‑1 (MQ=60)
                                                                             GAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGT                                                                       >  SRR19973074.788484/1‑150 (MQ=60)
                                                                              AAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGTAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTG                                                                      <  SRR19973074.3756197/150‑1 (MQ=60)
                                                                              AAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTG                                                                      <  SRR19973074.1262854/150‑1 (MQ=60)
                                                                               AGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                     <  SRR19973074.3976169/150‑1 (MQ=60)
                                                                               AGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGA                                                                     >  SRR19973074.3143747/1‑150 (MQ=60)
                                                                                 AACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAA                                                                   <  SRR19973074.1741352/150‑1 (MQ=60)
                                                                                  ACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAAT                                                                  <  SRR19973074.1941606/150‑1 (MQ=60)
                                                                                   CGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATA                                                                 >  SRR19973074.2459466/1‑150 (MQ=60)
                                                                                    GCGATGCGCTATATCGCGAAGTTGCGCATTTTATCATCGACGCAACAAACGTACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAA                                                                <  SRR19973074.413702/150‑1 (MQ=60)
                                                                                     CGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAG                                                               <  SRR19973074.3418598/150‑1 (MQ=60)
                                                                                     CGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAG                                                               >  SRR19973074.1348326/1‑150 (MQ=60)
                                                                                      GATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGG                                                              >  SRR19973074.1840456/1‑150 (MQ=60)
                                                                                      GATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGG                                                              >  SRR19973074.1382298/1‑150 (MQ=60)
                                                                                        TGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAA                                                            >  SRR19973074.3682639/1‑150 (MQ=60)
                                                                                           GCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAA                                                         <  SRR19973074.2691307/150‑1 (MQ=60)
                                                                                                ATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATG                                                    >  SRR19973074.1668058/1‑150 (MQ=60)
                                                                                                   GCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCA                                                 <  SRR19973074.4070467/150‑1 (MQ=60)
                                                                                                   GCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCA                                                 <  SRR19973074.1681721/150‑1 (MQ=60)
                                                                                                    CGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAA                                                >  SRR19973074.1370972/1‑150 (MQ=60)
                                                                                                    CGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAA                                                >  SRR19973074.1370847/1‑150 (MQ=60)
                                                                                                    CGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAA                                                >  SRR19973074.169486/1‑150 (MQ=60)
                                                                                                     GAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAAC                                               >  SRR19973074.3990940/1‑150 (MQ=60)
                                                                                                     GAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAAC                                               >  SRR19973074.1864065/1‑150 (MQ=60)
                                                                                                     GAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAAC                                               >  SRR19973074.1888338/1‑150 (MQ=60)
                                                                                                      AAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACG                                              <  SRR19973074.3893533/150‑1 (MQ=60)
                                                                                                            CGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCG                                        <  SRR19973074.2880904/150‑1 (MQ=60)
                                                                                                            CGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCG                                        <  SRR19973074.2880908/150‑1 (MQ=60)
                                                                                                             GCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGC                                       >  SRR19973074.2767674/1‑150 (MQ=60)
                                                                                                                TATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTT                                    <  SRR19973074.3094332/150‑1 (MQ=60)
                                                                                                                 ATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTA                                   >  SRR19973074.855680/1‑150 (MQ=60)
                                                                                                                   TATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATC                                 >  SRR19973074.1010663/1‑150 (MQ=60)
                                                                                                                   TATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATC                                 >  SRR19973074.1010571/1‑150 (MQ=60)
                                                                                                                     TCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCT                               >  SRR19973074.551848/1‑150 (MQ=60)
                                                                                                                      CATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTC                              >  SRR19973074.2551977/1‑150 (MQ=60)
                                                                                                                       ATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCG                             <  SRR19973074.3318474/150‑1 (MQ=60)
                                                                                                                         CGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTG                           >  SRR19973074.1949216/1‑150 (MQ=60)
                                                                                                                           ACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAA                         <  SRR19973074.712824/150‑1 (MQ=60)
                                                                                                                           ACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAA                         <  SRR19973074.394972/150‑1 (MQ=60)
                                                                                                                           ACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAA                         <  SRR19973074.395135/150‑1 (MQ=60)
                                                                                                                            CGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAG                        <  SRR19973074.2419109/150‑1 (MQ=60)
                                                                                                                              CAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCA                      <  SRR19973074.804889/150‑1 (MQ=60)
                                                                                                                                  AAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCATCGTGAAGCATATA                  >  SRR19973074.99934/1‑150 (MQ=60)
                                                                                                                                   AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                 >  SRR19973074.1274190/1‑150 (MQ=60)
                                                                                                                                   AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                 >  SRR19973074.1274228/1‑150 (MQ=60)
                                                                                                                                   AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                 >  SRR19973074.2080971/1‑150 (MQ=60)
                                                                                                                                   AACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAT                 >  SRR19973074.3371055/1‑150 (MQ=60)
                                                                                                                                     CGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAG               <  SRR19973074.4154381/150‑1 (MQ=60)
                                                                                                                                       AACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTG             <  SRR19973074.3038940/150‑1 (MQ=60)
                                                                                                                                       AACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTG             <  SRR19973074.2543197/150‑1 (MQ=60)
                                                                                                                                       AACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTG             <  SRR19973074.3038990/150‑1 (MQ=60)
                                                                                                                                        ACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGA            <  SRR19973074.37563/150‑1 (MQ=60)
                                                                                                                                        ACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGA            <  SRR19973074.976805/150‑1 (MQ=60)
                                                                                                                                        ACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGA            >  SRR19973074.2325981/1‑150 (MQ=60)
                                                                                                                                         CCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAC           >  SRR19973074.1614765/1‑150 (MQ=60)
                                                                                                                                         CCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAC           >  SRR19973074.708732/1‑150 (MQ=60)
                                                                                                                                         CCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAC           >  SRR19973074.3826177/1‑150 (MQ=60)
                                                                                                                                            AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT        <  SRR19973074.2343929/150‑1 (MQ=60)
                                                                                                                                            AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT        <  SRR19973074.2343938/150‑1 (MQ=60)
                                                                                                                                            AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT        <  SRR19973074.306962/150‑1 (MQ=60)
                                                                                                                                            AGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAT        <  SRR19973074.306971/150‑1 (MQ=60)
                                                                                                                                               CAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA     >  SRR19973074.2851059/1‑150 (MQ=60)
                                                                                                                                               CAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA     >  SRR19973074.2858243/1‑150 (MQ=60)
                                                                                                                                               CAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA     <  SRR19973074.1181507/150‑1 (MQ=60)
                                                                                                                                                  GTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA  >  SRR19973074.1560494/1‑150 (MQ=60)
                                                                                                                                                  GTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA  >  SRR19973074.1560464/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
TGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAA  >  CP009273/402192‑402487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑