Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,755,150 A→T 66.0% L300* (TTG→TAG)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,755,1500AT66.0% 248.7 / 220.7 212L300* (TTG→TAG) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base A (42/30);  new base T (60/80);  total (102/110)
Fisher's exact test for biased strand distribution p-value = 4.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 212 total aligned reads displayed.

CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACA  >  CP009273/1755005‑1755294
                                                                                                                                                 |                                                                                                                                                
cTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTAc                                                                                                                                                <  1:95275/148‑1 (MQ=255)
  gCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTg                                                                                                                                              <  2:1267621/148‑1 (MQ=255)
   cGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGt                                                                                                                                             <  2:1905087/148‑1 (MQ=255)
      tttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCgg                                                                                                                                          >  2:1028756/1‑148 (MQ=255)
      tttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCgg                                                                                                                                          >  2:213061/1‑148 (MQ=255)
       ttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGc                                                                                                                                         >  2:2997276/1‑148 (MQ=255)
          gTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGt                                                                                                                                      <  2:2672474/148‑1 (MQ=255)
           tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGtt                                                                                                                                     <  1:2449627/148‑1 (MQ=255)
           tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGtt                                                                                                                                     >  1:579064/1‑148 (MQ=255)
            aTCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTa                                                                                                                                    <  1:2470833/148‑1 (MQ=255)
            aTCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTa                                                                                                                                    <  2:123685/148‑1 (MQ=255)
            aTCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTa                                                                                                                                    >  2:245516/1‑148 (MQ=255)
            aTCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTa                                                                                                                                    >  2:2422600/1‑148 (MQ=255)
              ccACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACa                                                                                                                                  >  1:501736/1‑148 (MQ=255)
                  ttCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaaa                                                                                                                              <  1:1424027/148‑1 (MQ=255)
                   tCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAAc                                                                                                                             <  2:2540886/148‑1 (MQ=255)
                       ccAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCttt                                                                                                                         <  1:1723758/148‑1 (MQ=255)
                        cAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTa                                                                                                                        <  1:510791/148‑1 (MQ=255)
                        cAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTa                                                                                                                        >  2:2760753/1‑148 (MQ=255)
                        cAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTa                                                                                                                        >  1:965274/1‑148 (MQ=255)
                        cAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTa                                                                                                                        >  1:965238/1‑148 (MQ=255)
                            ttGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATtgt                                                                                                                    <  2:2275598/148‑1 (MQ=255)
                            ttGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATtgt                                                                                                                    >  2:2893648/1‑148 (MQ=255)
                             tGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATtgtg                                                                                                                   <  2:1197751/148‑1 (MQ=255)
                              gCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTgtgt                                                                                                                  <  2:3106277/148‑1 (MQ=255)
                                  aTCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcca                                                                                                              <  1:2605062/148‑1 (MQ=255)
                                   tCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccag                                                                                                             >  2:432756/1‑148 (MQ=255)
                                   tCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCCTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccag                                                                                                             >  2:1675901/1‑148 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            <  2:2266765/148‑1 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            <  2:962596/148‑1 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            <  1:2681/148‑1 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            <  1:766919/148‑1 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            >  2:2709572/1‑148 (MQ=255)
                                       cagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagccag                                                                                                         >  1:104300/1‑148 (MQ=255)
                                       cagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagccag                                                                                                         >  1:972070/1‑148 (MQ=255)
                                       cagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCATTGTAGCTGACGATAGTTTTGTGCAAATTTCGGCTGTTACAAAAAACTTTATTGTTTTacagccag                                                                                                         >  1:2690155/1‑148 (MQ=255)
                                          aTTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTa                                                                                                      >  2:2578179/1‑148 (MQ=255)
                                          aTTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTa                                                                                                      >  1:1686991/1‑148 (MQ=255)
                                             gttgttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACgg                                                                                                   <  1:1877634/148‑1 (MQ=255)
                                              ttgttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACggg                                                                                                  <  1:823283/148‑1 (MQ=255)
                                                 ttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTAt                                                                                               <  1:2798285/148‑1 (MQ=255)
                                                    gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                            <  1:3331112/148‑1 (MQ=255)
                                                    gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                            >  2:2481298/1‑148 (MQ=255)
                                                     tCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcaca                                                                                           <  1:1028756/148‑1 (MQ=255)
                                                      cATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcacac                                                                                          <  2:3502302/148‑1 (MQ=255)
                                                        tCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCacacac                                                                                        >  2:3337898/1‑148 (MQ=255)
                                                         cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACAcc                                                                                       >  2:701275/1‑148 (MQ=255)
                                                         cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACAcc                                                                                       <  1:435491/148‑1 (MQ=255)
                                                            aCCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    <  1:1894371/148‑1 (MQ=255)
                                                            aCCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    <  1:3472504/148‑1 (MQ=255)
                                                            aCCATACGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    <  2:2088502/148‑1 (MQ=255)
                                                            aCCAGCCGTTGTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    <  2:721043/148‑1 (MQ=255)
                                                              cATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGtt                                                                                  <  1:3121869/148‑1 (MQ=255)
                                                                tCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGtttt                                                                                <  1:2437260/148‑1 (MQ=255)
                                                                 ccGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttttt                                                                               <  1:1871448/148‑1 (MQ=255)
                                                                   gTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCa                                                                             <  1:2873342/148‑1 (MQ=255)
                                                                   gTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCa                                                                             <  1:2873408/148‑1 (MQ=255)
                                                                     tttGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCAcc                                                                           >  2:420099/1‑148 (MQ=255)
                                                                       tGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCgg                                                                         >  1:3342260/1‑148 (MQ=255)
                                                                         aTGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCa                                                                       >  1:1433319/1‑148 (MQ=255)
                                                                          tGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCAt                                                                      <  1:1232889/148‑1 (MQ=255)
                                                                           ggCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATc                                                                     >  1:1163668/1‑148 (MQ=255)
                                                                             cGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGc                                                                   <  1:1542890/148‑1 (MQ=255)
                                                                                 ctgcGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATAACACACCTCGTTTTTCAACGGCATCGCCAgg                                                               >  1:2308501/1‑148 (MQ=255)
                                                                                        aCGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGAAGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGa                                                        >  2:540470/1‑148 (MQ=255)
                                                                                         cGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                       <  2:3121862/148‑1 (MQ=255)
                                                                                         cGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                       <  1:2673977/148‑1 (MQ=255)
                                                                                               tCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                 >  2:3211784/1‑148 (MQ=255)
                                                                                               tCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                 >  1:1804189/1‑148 (MQ=255)
                                                                                               tCAAACCGTTAAATACCGCGCGGCCTGTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTGATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                 <  2:2213015/148‑1 (MQ=255)
                                                                                                cAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTAGGGGTCTCACACACCTCGTTTGTCACCGGCATCGCCCGGCTATTGATCCGCGGc                                                >  2:1862996/1‑148 (MQ=255)
                                                                                                 aaaCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCg                                               <  1:1227096/148‑1 (MQ=255)
                                                                                                   aCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTg                                             >  1:1468838/1‑148 (MQ=255)
                                                                                                      gTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTg                                          >  2:876885/1‑148 (MQ=255)
                                                                                                      gTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTg                                          <  2:1758334/148‑1 (MQ=255)
                                                                                                        tAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGtt                                        >  2:2184096/1‑148 (MQ=255)
                                                                                                          aaTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGtttt                                      <  1:680660/148‑1 (MQ=255)
                                                                                                             aCCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGc                                   >  1:2527721/1‑148 (MQ=255)
                                                                                                             aCCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGc                                   >  1:3539740/1‑148 (MQ=255)
                                                                                                                 cgcgGCCTTGGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCAtt                               <  2:542103/148‑1 (MQ=255)
                                                                                                                   cgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGa                             <  2:1686991/148‑1 (MQ=255)
                                                                                                                   cgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGa                             <  2:1736939/148‑1 (MQ=255)
                                                                                                                     gCCTGTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTGTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGt                           <  2:3307331/148‑1 (MQ=255)
                                                                                                                      ccTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                          >  1:288042/1‑148 (MQ=255)
                                                                                                                       cTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTg                         >  1:348720/1‑148 (MQ=255)
                                                                                                                        tttGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGc                        >  1:529759/1‑148 (MQ=255)
                                                                                                                         ttGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCg                       >  2:52642/1‑148 (MQ=255)
                                                                                                                          tGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGt                      <  1:1402774/148‑1 (MQ=255)
                                                                                                                           gTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTa                     <  1:432756/148‑1 (MQ=255)
                                                                                                                              gCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTg                  >  2:2913863/1‑148 (MQ=255)
                                                                                                                               cTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTgg                 >  2:2356262/1‑148 (MQ=255)
                                                                                                                                 gACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg               <  2:1769500/148‑1 (MQ=255)
                                                                                                                                 gACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg               <  1:2481271/148‑1 (MQ=255)
                                                                                                                                     aTAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt           >  1:2110103/1‑148 (MQ=255)
                                                                                                                                     aTAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTGTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt           <  2:778723/148‑1 (MQ=255)
                                                                                                                                      tAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg          >  1:2729407/1‑148 (MQ=255)
                                                                                                                                       aGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTgtgt         >  1:2137413/1‑148 (MQ=255)
                                                                                                                                       aGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTgtgt         >  1:3252064/1‑148 (MQ=255)
                                                                                                                                         ttttGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTGCGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCg       >  1:3465735/1‑148 (MQ=255)
                                                                                                                                           ttGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTa     <  1:1675901/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACA  >  CP009273/1755005‑1755294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTG  >  CP009273/1755002‑1755297
                                                                                                                                                    |                                                                                                                                                   
CAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTA                                                                                                                                                    <  SRR19973066.1359977/150‑1 (MQ=60)
   CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTG                                                                                                                                                 <  SRR19973066.3450604/150‑1 (MQ=60)
   CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTG                                                                                                                                                 <  SRR19973066.96301/150‑1 (MQ=60)
            CGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTT                                                                                                                                        >  SRR19973066.427841/1‑150 (MQ=60)
            CGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTT                                                                                                                                        >  SRR19973066.583128/1‑150 (MQ=60)
              TATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTAC                                                                                                                                      <  SRR19973066.2466318/150‑1 (MQ=60)
               ATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACA                                                                                                                                     <  SRR19973066.2487672/150‑1 (MQ=60)
               ATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACA                                                                                                                                     <  SRR19973066.2487677/150‑1 (MQ=60)
               ATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACA                                                                                                                                     >  SRR19973066.505463/1‑150 (MQ=60)
                 CCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAA                                                                                                                                   >  SRR19973066.492394/1‑150 (MQ=60)
                   ACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAA                                                                                                                                 >  SRR19973066.974011/1‑150 (MQ=60)
                     TTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACC                                                                                                                               <  SRR19973066.1434057/150‑1 (MQ=60)
                     TTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACC                                                                                                                               <  SRR19973066.1434208/150‑1 (MQ=60)
                       CCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTT                                                                                                                             <  SRR19973066.2759692/150‑1 (MQ=60)
                        CGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTATGCTACTGTCGGCTGTTACAAAAACCTTT                                                                                                                            <  SRR19973066.2653815/150‑1 (MQ=60)
                         GCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTA                                                                                                                           >  SRR19973066.972332/1‑150 (MQ=60)
                         GCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTA                                                                                                                           >  SRR19973066.972368/1‑150 (MQ=60)
                         GCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAACCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTA                                                                                                                           >  SRR19973066.972362/1‑150 (MQ=60)
                          CCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTAT                                                                                                                          <  SRR19973066.1736231/150‑1 (MQ=60)
                           CAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATT                                                                                                                         <  SRR19973066.514572/150‑1 (MQ=60)
                           CAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATT                                                                                                                         <  SRR19973066.514571/150‑1 (MQ=60)
                           CAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATT                                                                                                                         <  SRR19973066.514569/150‑1 (MQ=60)
                                   CCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCA                                                                                                                 >  SRR19973066.1932863/1‑150 (MQ=60)
                                    CATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAG                                                                                                                >  SRR19973066.1471155/1‑150 (MQ=60)
                                     ATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGC                                                                                                               <  SRR19973066.2622681/150‑1 (MQ=60)
                                       CAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCA                                                                                                             <  SRR19973066.2716/150‑1 (MQ=60)
                                       CAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCA                                                                                                             <  SRR19973066.3501578/150‑1 (MQ=60)
                                       CAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCA                                                                                                             <  SRR19973066.772603/150‑1 (MQ=60)
                                        AGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAG                                                                                                            >  SRR19973066.105438/1‑150 (MQ=60)
                                        AGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAG                                                                                                            >  SRR19973066.105394/1‑150 (MQ=60)
                                        AGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAG                                                                                                            >  SRR19973066.105323/1‑150 (MQ=60)
                                        AGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAG                                                                                                            >  SRR19973066.2707480/1‑150 (MQ=60)
                                        AGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAG                                                                                                            >  SRR19973066.979220/1‑150 (MQ=60)
                                        AGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCATTGTAGCTGACGATAGTTTTGTGCAAATTTCGGCTGTTACAAAAAACTTTATTGTTTTACAGCCAG                                                                                                            >  SRR19973066.2708112/1‑150 (MQ=60)
                                           AGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTA                                                                                                         >  SRR19973066.1699271/1‑150 (MQ=60)
                                           AGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTA                                                                                                         >  SRR19973066.1699157/1‑150 (MQ=60)
                                                GTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGT                                                                                                    <  SRR19973066.1891195/150‑1 (MQ=60)
                                                GTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGT                                                                                                    <  SRR19973066.1891042/150‑1 (MQ=60)
                                                GTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGT                                                                                                    <  SRR19973066.1890773/150‑1 (MQ=60)
                                                 TTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTA                                                                                                   <  SRR19973066.829298/150‑1 (MQ=60)
                                                 TTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTA                                                                                                   <  SRR19973066.829296/150‑1 (MQ=60)
                                                    TTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCA                                                                                                <  SRR19973066.2817121/150‑1 (MQ=60)
                                                    TTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCA                                                                                                <  SRR19973066.2817145/150‑1 (MQ=60)
                                                       GTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACAC                                                                                             <  SRR19973066.3352632/150‑1 (MQ=60)
                                                       GTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACAC                                                                                             <  SRR19973066.3353261/150‑1 (MQ=60)
                                                        TCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACA                                                                                            <  SRR19973066.1036330/150‑1 (MQ=60)
                                                        TCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACA                                                                                            <  SRR19973066.2590421/150‑1 (MQ=60)
                                                            CTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTC                                                                                        <  SRR19973066.438865/150‑1 (MQ=60)
                                                             TGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCG                                                                                       >  SRR19973066.1782451/1‑150 (MQ=60)
                                                               ACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTT                                                                                     <  SRR19973066.1907881/150‑1 (MQ=60)
                                                               ACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTT                                                                                     <  SRR19973066.3495692/150‑1 (MQ=60)
                                                               ACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTT                                                                                     <  SRR19973066.1975360/150‑1 (MQ=60)
                                                                 CATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTT                                                                                   >  SRR19973066.2587006/1‑150 (MQ=60)
                                                                 CATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTT                                                                                   <  SRR19973066.3142715/150‑1 (MQ=60)
                                                                   TCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTC                                                                                 <  SRR19973066.2457438/150‑1 (MQ=60)
                                                                   TCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTC                                                                                 <  SRR19973066.2453860/150‑1 (MQ=60)
                                                                    CCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCA                                                                                <  SRR19973066.1884813/150‑1 (MQ=60)
                                                                      GTTTTGATGGCGTGCTGCGCGAGGTTGATCTCACCGTTAAAGCCCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTTGGGGTATCTCACACCTCGTTTTTCACC                                                                              <  SRR19973066.2892609/150‑1 (MQ=60)
                                                                      GTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACC                                                                              <  SRR19973066.2892607/150‑1 (MQ=60)
                                                                      GTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACC                                                                              <  SRR19973066.2892673/150‑1 (MQ=60)
                                                                        TTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGG                                                                            >  SRR19973066.3364509/1‑150 (MQ=60)
                                                                          TGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCA                                                                          >  SRR19973066.1443543/1‑150 (MQ=60)
                                                                          TGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCA                                                                          >  SRR19973066.1442825/1‑150 (MQ=60)
                                                                            ATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATC                                                                        >  SRR19973066.1171916/1‑150 (MQ=60)
                                                                             TGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCG                                                                       <  SRR19973066.1241647/150‑1 (MQ=60)
                                                                                CGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCA                                                                    <  SRR19973066.1553925/150‑1 (MQ=60)
                                                                                  TGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGG                                                                  >  SRR19973066.3360346/1‑150 (MQ=60)
                                                                                  TGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATAACACACCTCGTTTTTCAACGGCATCGCCAGG                                                                  >  SRR19973066.2324308/1‑150 (MQ=60)
                                                                                          GACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAT                                                          >  SRR19973066.227467/1‑150 (MQ=60)
                                                                                            CGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCC                                                        <  SRR19973066.2691841/150‑1 (MQ=60)
                                                                                             GTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCG                                                       >  SRR19973066.2146806/1‑150 (MQ=60)
                                                                                                GATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAG                                                    >  SRR19973066.1817155/1‑150 (MQ=60)
                                                                                                GATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAG                                                    >  SRR19973066.1817000/1‑150 (MQ=60)
                                                                                                   CAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGT                                                 >  SRR19973066.759195/1‑150 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                <  SRR19973066.772638/150‑1 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                >  SRR19973066.677584/1‑150 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                >  SRR19973066.959786/1‑150 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                >  SRR19973066.1479199/1‑150 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                <  SRR19973066.153096/150‑1 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                <  SRR19973066.1235798/150‑1 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                <  SRR19973066.1235791/150‑1 (MQ=60)
                                                                                                    AAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                <  SRR19973066.1535702/150‑1 (MQ=60)
                                                                                                    AAACCGTTAAAGACCGCGCGGCCTGTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTG                                                <  SRR19973066.1621184/150‑1 (MQ=60)
                                                                                                             AATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCG                                       <  SRR19973066.685650/150‑1 (MQ=60)
                                                                                                              ATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGC                                      >  SRR19973066.2544910/1‑150 (MQ=60)
                                                                                                              ATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGC                                      >  SRR19973066.3563260/1‑150 (MQ=60)
                                                                                                                  CGCGCGGCCTTTGTCGCTGACGATATTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATT                                  <  SRR19973066.3157816/150‑1 (MQ=60)
                                                                                                                  CGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATT                                  <  SRR19973066.3542766/150‑1 (MQ=60)
                                                                                                                     GCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAG                               <  SRR19973066.2435516/150‑1 (MQ=60)
                                                                                                                       GGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTT                             >  SRR19973066.290007/1‑150 (MQ=60)
                                                                                                                       GGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTT                             >  SRR19973066.290112/1‑150 (MQ=60)
                                                                                                                       GGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTT                             >  SRR19973066.3490119/1‑150 (MQ=60)
                                                                                                                        GCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTG                            >  SRR19973066.351438/1‑150 (MQ=60)
                                                                                                                         CCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGC                           >  SRR19973066.533676/1‑150 (MQ=60)
                                                                                                                         CCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGC                           >  SRR19973066.533629/1‑150 (MQ=60)
                                                                                                                             TGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTAC                       <  SRR19973066.1214902/150‑1 (MQ=60)
                                                                                                                             TGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTAC                       <  SRR19973066.1412874/150‑1 (MQ=60)
                                                                                                                             TGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTAC                       >  SRR19973066.810715/1‑150 (MQ=60)
                                                                                                                              GTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACT                      <  SRR19973066.436109/150‑1 (MQ=60)
                                                                                                                              GTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACT                      >  SRR19973066.1383490/1‑150 (MQ=60)
                                                                                                                              GTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGGTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTTCT                      <  SRR19973066.436290/150‑1 (MQ=60)
                                                                                                                                    GACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTT                <  SRR19973066.2498195/150‑1 (MQ=60)
                                                                                                                                    GACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTT                <  SRR19973066.2497552/150‑1 (MQ=60)
                                                                                                                                    GACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTT                <  SRR19973066.2498222/150‑1 (MQ=60)
                                                                                                                                      CGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGT              >  SRR19973066.2124673/1‑150 (MQ=60)
                                                                                                                                       GATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTG             >  SRR19973066.2747719/1‑150 (MQ=60)
                                                                                                                                       GATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTG             >  SRR19973066.2747761/1‑150 (MQ=60)
                                                                                                                                        ATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGT            >  SRR19973066.2695842/1‑150 (MQ=60)
                                                                                                                                        ATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGT            >  SRR19973066.3273483/1‑150 (MQ=60)
                                                                                                                                        ATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGT            >  SRR19973066.2152240/1‑150 (MQ=60)
                                                                                                                                          AGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTGCGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCG          >  SRR19973066.311993/1‑150 (MQ=60)
                                                                                                                                          AGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTGCGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCG          >  SRR19973066.1714400/1‑150 (MQ=60)
                                                                                                                                          AGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTGCGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCG          >  SRR19973066.312062/1‑150 (MQ=60)
                                                                                                                                          AGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTGCGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCG          >  SRR19973066.3488901/1‑150 (MQ=60)
                                                                                                                                              TTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAAC      <  SRR19973066.1688111/150‑1 (MQ=60)
                                                                                                                                                 TGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACT   <  SRR19973066.421249/150‑1 (MQ=60)
                                                                                                                                                  GCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTG  <  SRR19973066.3488565/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTG  >  CP009273/1755002‑1755297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑