Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,755,150 A→T 100% L300* (TTG→TAG)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,755,1500AT95.0% 563.0 / 15.1 180L300* (TTG→TAG) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base A (7/2);  new base T (89/82);  total (96/84)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 180 total aligned reads displayed.

CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACA  >  CP009273/1755005‑1755294
                                                                                                                                                 |                                                                                                                                                
cTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTAc                                                                                                                                                >  1:1528600/1‑148 (MQ=255)
  gCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTg                                                                                                                                              >  2:3482622/1‑148 (MQ=255)
      tttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCgg                                                                                                                                          >  2:581274/1‑148 (MQ=255)
       ttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGc                                                                                                                                         <  2:622645/148‑1 (MQ=255)
        tCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCt                                                                                                                                        <  2:321517/148‑1 (MQ=255)
         cGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTg                                                                                                                                       <  2:250076/148‑1 (MQ=255)
         cGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTg                                                                                                                                       <  2:2022545/148‑1 (MQ=255)
           tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGtt                                                                                                                                     <  2:1330344/148‑1 (MQ=255)
             tCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTAc                                                                                                                                   >  1:646675/1‑148 (MQ=255)
                aCTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaa                                                                                                                                >  1:2925795/1‑148 (MQ=255)
                 cTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaa                                                                                                                               <  1:3260413/148‑1 (MQ=255)
                 cTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaa                                                                                                                               <  1:1989825/148‑1 (MQ=255)
                 cTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATATGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaa                                                                                                                               >  2:350268/1‑148 (MQ=255)
                  ttCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACaaaaa                                                                                                                              >  1:576354/1‑148 (MQ=255)
                       ccAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCttt                                                                                                                         >  2:2862337/1‑148 (MQ=255)
                       ccAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCttt                                                                                                                         <  2:2993058/148‑1 (MQ=255)
                        cAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTa                                                                                                                        >  1:1368131/1‑148 (MQ=255)
                              gCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTgtgt                                                                                                                  <  2:2683367/148‑1 (MQ=255)
                              gCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTgtgt                                                                                                                  <  1:2780223/148‑1 (MQ=255)
                               cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                 <  2:2272801/148‑1 (MQ=255)
                               cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                 >  2:156721/1‑148 (MQ=255)
                               cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                 >  1:567689/1‑148 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  2:1801756/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  2:2321329/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  1:983322/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                <  2:949456/148‑1 (MQ=255)
                                ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTc                                                                                                                >  1:2174785/1‑148 (MQ=255)
                                 cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                               >  2:299546/1‑148 (MQ=255)
                                 cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                               >  2:299566/1‑148 (MQ=255)
                                 cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                               <  2:2448829/148‑1 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            >  2:3218885/1‑148 (MQ=255)
                                    cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                            >  1:3253359/1‑148 (MQ=255)
                                      gcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagcca                                                                                                          >  2:569966/1‑148 (MQ=255)
                                      gcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagcca                                                                                                          >  2:570433/1‑148 (MQ=255)
                                       cagATTGTTGATGGTCATCTGACCATCCGTTTTGAATGCGTGCTGCGCGACGTTGAACAAACCGTTAAATACCGCGCGCCCATTGTCGCTGACGATAGTTTTGAGCTACTGTCGGCTGTAACAAAAACCTTTATTTTGTTccagacag                                                                                                         >  2:877257/1‑148 (MQ=255)
                                         gATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGt                                                                                                       >  1:1873911/1‑148 (MQ=255)
                                          aTTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTa                                                                                                      <  2:763483/148‑1 (MQ=255)
                                                  tgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATc                                                                                              >  2:3300630/1‑148 (MQ=255)
                                                  tgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATc                                                                                              >  2:691114/1‑148 (MQ=255)
                                                    gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                            >  1:2596860/1‑148 (MQ=255)
                                                    gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                            >  2:804474/1‑148 (MQ=255)
                                                        tCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCacacac                                                                                        <  2:1368131/148‑1 (MQ=255)
                                                         cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACAcc                                                                                       >  2:2627346/1‑148 (MQ=255)
                                                         cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACAcc                                                                                       <  1:102858/148‑1 (MQ=255)
                                                            aCCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    >  1:2863064/1‑148 (MQ=255)
                                                            aCCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCg                                                                                    >  1:2909761/1‑148 (MQ=255)
                                                             ccATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGt                                                                                   >  1:3498370/1‑148 (MQ=255)
                                                             ccATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGt                                                                                   <  2:2519155/148‑1 (MQ=255)
                                                               aTCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttt                                                                                 >  1:1228072/1‑148 (MQ=255)
                                                               aTCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttt                                                                                 >  1:1228067/1‑148 (MQ=255)
                                                               aTCCGTTTTGATGGCGTGCTGCGCGAAGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCTCTGACGATATTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGttt                                                                                 <  1:125550/148‑1 (MQ=255)
                                                                          tGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCAt                                                                      <  1:510237/148‑1 (MQ=255)
                                                                                    cgcgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTa                                                            <  2:2578698/148‑1 (MQ=255)
                                                                                    cgcgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTa                                                            <  1:1595658/148‑1 (MQ=255)
                                                                                      cgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTAtt                                                          <  1:156721/148‑1 (MQ=255)
                                                                                        aCGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGa                                                        >  1:118280/1‑148 (MQ=255)
                                                                                         cGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                       >  1:999683/1‑148 (MQ=255)
                                                                                         cGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                       <  2:2150572/148‑1 (MQ=255)
                                                                                          gTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATc                                                      <  2:3458796/148‑1 (MQ=255)
                                                                                           ttGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATcc                                                     >  1:1815894/1‑148 (MQ=255)
                                                                                           ttGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATcc                                                     >  2:1097932/1‑148 (MQ=255)
                                                                                           ttGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATcc                                                     >  1:442350/1‑148 (MQ=255)
                                                                                             gATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGc                                                   <  1:3343398/148‑1 (MQ=255)
                                                                                               tCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                 >  1:3076585/1‑148 (MQ=255)
                                                                                                cAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGc                                                >  2:3026942/1‑148 (MQ=255)
                                                                                                  aaCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGt                                              >  2:46844/1‑148 (MQ=255)
                                                                                                   aCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTg                                             >  2:2188589/1‑148 (MQ=255)
                                                                                                     cGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCt                                           >  2:1093586/1‑148 (MQ=255)
                                                                                                      gTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTg                                          >  2:2515922/1‑148 (MQ=255)
                                                                                                        tAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGtt                                        >  1:2048179/1‑148 (MQ=255)
                                                                                                        tAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGtt                                        >  2:1794276/1‑148 (MQ=255)
                                                                                                         aaaTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGttt                                       >  1:285902/1‑148 (MQ=255)
                                                                                                           aTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                     >  1:1113598/1‑148 (MQ=255)
                                                                                                           aTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                     >  2:1349036/1‑148 (MQ=255)
                                                                                                           aTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                     >  2:3152193/1‑148 (MQ=255)
                                                                                                                   cgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTAGTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGa                             <  2:3066727/148‑1 (MQ=255)
                                                                                                                     gCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGt                           >  1:882594/1‑148 (MQ=255)
                                                                                                                     gCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGt                           <  2:1615534/148‑1 (MQ=255)
                                                                                                                      ccTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                          >  1:2481737/1‑148 (MQ=255)
                                                                                                                      ccTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                          <  2:3355027/148‑1 (MQ=255)
                                                                                                                        tttGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGc                        <  1:109845/148‑1 (MQ=255)
                                                                                                                          tGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGt                      <  1:2550483/148‑1 (MQ=255)
                                                                                                                           gTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGGTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTa                     <  2:1962687/148‑1 (MQ=255)
                                                                                                                            tCGCTGACGATAGTTTTGTGCTACTGTCCGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTAc                    <  2:1467770/148‑1 (MQ=255)
                                                                                                                             cGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACt                   >  1:3130160/1‑148 (MQ=255)
                                                                                                                              gCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTg                  <  1:1016348/148‑1 (MQ=255)
                                                                                                                               cTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTgg                 >  2:2755398/1‑148 (MQ=255)
                                                                                                                                tGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgt                >  1:2370441/1‑148 (MQ=255)
                                                                                                                                 gACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg               >  1:3216954/1‑148 (MQ=255)
                                                                                                                                 gACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg               >  1:3217176/1‑148 (MQ=255)
                                                                                                                                  aCGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtgt              <  2:1815894/148‑1 (MQ=255)
                                                                                                                                  aCGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtgt              <  1:3459415/148‑1 (MQ=255)
                                                                                                                                   cGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgtt             <  1:691036/148‑1 (MQ=255)
                                                                                                                                     aTAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt           >  2:2946708/1‑148 (MQ=255)
                                                                                                                                     aTAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt           >  2:678784/1‑148 (MQ=255)
                                                                                                                                      tAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTCTTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg          >  1:3306877/1‑148 (MQ=255)
                                                                                                                                      tAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg          >  1:1567475/1‑148 (MQ=255)
                                                                                                                                       aGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTCCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTgtgt         <  2:576927/148‑1 (MQ=255)
                                                                                                                                         ttttGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCg       <  1:2622756/148‑1 (MQ=255)
                                                                                                                                              tgCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAaca  <  2:646675/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACA  >  CP009273/1755005‑1755294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCG  >  CP009273/1755003‑1755299
                                                                                                                                                   |                                                                                                                                                     
AGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTAC                                                                                                                                                     >  SRR19973065.1538650/1‑150 (MQ=60)
 GCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACT                                                                                                                                                    <  SRR19973065.3131928/150‑1 (MQ=60)
 GCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACT                                                                                                                                                    <  SRR19973065.3481913/150‑1 (MQ=60)
 GCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACT                                                                                                                                                    <  SRR19973065.3481874/150‑1 (MQ=60)
      GGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGG                                                                                                                                               >  SRR19973065.263632/1‑150 (MQ=60)
             TATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTAC                                                                                                                                        >  SRR19973065.650927/1‑150 (MQ=60)
             TATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTAC                                                                                                                                        >  SRR19973065.1157731/1‑150 (MQ=60)
              ATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACA                                                                                                                                       <  SRR19973065.1827720/150‑1 (MQ=60)
                CCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAA                                                                                                                                     >  SRR19973065.2943569/1‑150 (MQ=60)
                  ACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAA                                                                                                                                   >  SRR19973065.579969/1‑150 (MQ=60)
                  ACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAA                                                                                                                                   >  SRR19973065.1277853/1‑150 (MQ=60)
                  ACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAA                                                                                                                                   >  SRR19973065.580544/1‑150 (MQ=60)
                   CTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAAC                                                                                                                                  <  SRR19973065.3280152/150‑1 (MQ=60)
                   CTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAAC                                                                                                                                  <  SRR19973065.2002734/150‑1 (MQ=60)
                        GCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTA                                                                                                                             >  SRR19973065.1377170/1‑150 (MQ=60)
                          CAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATT                                                                                                                           <  SRR19973065.2062203/150‑1 (MQ=60)
                               TGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTT                                                                                                                      >  SRR19973065.571288/1‑150 (MQ=60)
                                GCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTC                                                                                                                     <  SRR19973065.2797474/150‑1 (MQ=60)
                                GCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTC                                                                                                                     >  SRR19973065.2188758/1‑150 (MQ=60)
                                GCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTC                                                                                                                     >  SRR19973065.2188737/1‑150 (MQ=60)
                                  CCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCA                                                                                                                   <  SRR19973065.989854/150‑1 (MQ=60)
                                    ATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGC                                                                                                                 >  SRR19973065.3272752/1‑150 (MQ=60)
                                    ATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGC                                                                                                                 >  SRR19973065.3273028/1‑150 (MQ=60)
                                         CAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGT                                                                                                            >  SRR19973065.1886249/1‑150 (MQ=60)
                                               GTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGT                                                                                                      >  SRR19973065.916792/1‑150 (MQ=60)
                                                    TGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCAC                                                                                                 >  SRR19973065.2613002/1‑150 (MQ=60)
                                                        CATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCATACCGTTAAATACCGCGCGGCCTTTTTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACATAAACCTTTCTTGTGTTCCAGCCAGGTACGGGTATCACACAC                                                                                             <  SRR19973065.2550897/150‑1 (MQ=60)
                                                           CTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTC                                                                                          <  SRR19973065.159023/150‑1 (MQ=60)
                                                           CTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTC                                                                                          <  SRR19973065.3051612/150‑1 (MQ=60)
                                                           CTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTC                                                                                          <  SRR19973065.103807/150‑1 (MQ=60)
                                                            TGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCG                                                                                         >  SRR19973065.2927482/1‑150 (MQ=60)
                                                            TGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCG                                                                                         >  SRR19973065.2927478/1‑150 (MQ=60)
                                                            TGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCG                                                                                         >  SRR19973065.2880638/1‑150 (MQ=60)
                                                             GACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGT                                                                                        >  SRR19973065.3519625/1‑150 (MQ=60)
                                                               CCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTT                                                                                      >  SRR19973065.1236084/1‑150 (MQ=60)
                                                               CCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTT                                                                                      >  SRR19973065.1236089/1‑150 (MQ=60)
                                                               CCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTT                                                                                      >  SRR19973065.1239132/1‑150 (MQ=60)
                                                                 ATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTT                                                                                    >  SRR19973065.3221130/1‑150 (MQ=60)
                                                                 ATCCGTTTTGATGGCGTGCTGCGCGAAGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCTCTGACGATATTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTT                                                                                    <  SRR19973065.126635/150‑1 (MQ=60)
                                                                    CGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCAC                                                                                 >  SRR19973065.1567796/1‑150 (MQ=60)
                                                                    CGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCAC                                                                                 <  SRR19973065.1222819/150‑1 (MQ=60)
                                                                     GTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACC                                                                                >  SRR19973065.2743617/1‑150 (MQ=60)
                                                                         TGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCA                                                                            <  SRR19973065.3314408/150‑1 (MQ=60)
                                                                            TGGCGTGCTGCGCGACGTTGATCACACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCG                                                                         <  SRR19973065.1415132/150‑1 (MQ=60)
                                                                            TGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCG                                                                         <  SRR19973065.513619/150‑1 (MQ=60)
                                                                            TGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCG                                                                         <  SRR19973065.1840115/150‑1 (MQ=60)
                                                                                   CTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCT                                                                  <  SRR19973065.3446606/150‑1 (MQ=60)
                                                                                      CGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATT                                                               <  SRR19973065.1606316/150‑1 (MQ=60)
                                                                                        CGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGA                                                             >  SRR19973065.119317/1‑150 (MQ=60)
                                                                                        CGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGA                                                             <  SRR19973065.158020/150‑1 (MQ=60)
                                                                                         GACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAT                                                            >  SRR19973065.1006324/1‑150 (MQ=60)
                                                                                           CGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCC                                                          >  SRR19973065.1827854/1‑150 (MQ=60)
                                                                                           CGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCC                                                          >  SRR19973065.445414/1‑150 (MQ=60)
                                                                                           CGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCC                                                          >  SRR19973065.32973/1‑150 (MQ=60)
                                                                                               GATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAG                                                      <  SRR19973065.3363824/150‑1 (MQ=60)
                                                                                               GATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAG                                                      >  SRR19973065.3095377/1‑150 (MQ=60)
                                                                                               GATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAG                                                      >  SRR19973065.3097184/1‑150 (MQ=60)
                                                                                                       CGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGT                                              >  SRR19973065.558560/1‑150 (MQ=60)
                                                                                                        GTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTT                                             >  SRR19973065.2061271/1‑150 (MQ=60)
                                                                                                         TTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTT                                            >  SRR19973065.287783/1‑150 (MQ=60)
                                                                                                           AAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTC                                          >  SRR19973065.1120875/1‑150 (MQ=60)
                                                                                                           AAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTC                                          >  SRR19973065.1120783/1‑150 (MQ=60)
                                                                                                                CCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCAT                                     <  SRR19973065.3503813/150‑1 (MQ=60)
                                                                                                                    GCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAG                                 <  SRR19973065.2865659/150‑1 (MQ=60)
                                                                                                                     CGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGT                                >  SRR19973065.888213/1‑150 (MQ=60)
                                                                                                                      GGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTT                               >  SRR19973065.2497358/1‑150 (MQ=60)
                                                                                                                      GGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTT                               <  SRR19973065.429147/150‑1 (MQ=60)
                                                                                                                      GGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTT                               >  SRR19973065.2497226/1‑150 (MQ=60)
                                                                                                                          TTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGT                           <  SRR19973065.110252/150‑1 (MQ=60)
                                                                                                                          TTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGT                           <  SRR19973065.110837/150‑1 (MQ=60)
                                                                                                                            TGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTAC                         <  SRR19973065.2566449/150‑1 (MQ=60)
                                                                                                                             GTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACT                        >  SRR19973065.3149209/1‑150 (MQ=60)
                                                                                                                                GCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGT                     >  SRR19973065.2385224/1‑150 (MQ=60)
                                                                                                                                GCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGT                     <  SRR19973065.1023079/150‑1 (MQ=60)
                                                                                                                                 CTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTG                    >  SRR19973065.471972/1‑150 (MQ=60)
                                                                                                                                 CTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTG                    >  SRR19973065.3236300/1‑150 (MQ=60)
                                                                                                                                 CTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTG                    >  SRR19973065.3236522/1‑150 (MQ=60)
                                                                                                                                    ACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTG                 <  SRR19973065.3480491/150‑1 (MQ=60)
                                                                                                                                     CGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGT                >  SRR19973065.1916390/1‑150 (MQ=60)
                                                                                                                                     CGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGT                <  SRR19973065.695641/150‑1 (MQ=60)
                                                                                                                                     CGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGT                <  SRR19973065.695719/150‑1 (MQ=60)
                                                                                                                                      GATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTCTTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTG               >  SRR19973065.3327028/1‑150 (MQ=60)
                                                                                                                                      GATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTG               >  SRR19973065.1577896/1‑150 (MQ=60)
                                                                                                                                           TTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTA          <  SRR19973065.2638988/150‑1 (MQ=60)
                                                                                                                                           TTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTA          <  SRR19973065.683307/150‑1 (MQ=60)
                                                                                                                                               GTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACAC      >  SRR19973065.1883588/1‑150 (MQ=60)
                                                                                                                                                TGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACT     <  SRR19973065.2879908/150‑1 (MQ=60)
                                                                                                                                                   TACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCG  <  SRR19973065.1339585/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACTGCG  >  CP009273/1755003‑1755299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑