Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,782,065 A→T 52.6% N122Y (AAT→TAT)  ppsR → bifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,782,0650AT52.6% 26.0 / 237.1 169N122Y (AAT→TAT) ppsRbifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (37/40);  new base T (38/45);  total (80/89)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

ATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG  >  CP009273/1781918‑1782212
                                                                                                                                                   |                                                                                                                                                   
aTCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCtaata                                                                                                                                                     <  1:2451427/148‑1 (MQ=255)
 tCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCTTACCCATGGCCTGAACCCtaataa                                                                                                                                                    >  1:340596/1‑148 (MQ=255)
  cGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaattat                                                                                                                                                   >  1:2916822/1‑148 (MQ=255)
    tgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTAtct                                                                                                                                                 <  2:1264074/148‑1 (MQ=255)
    tgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAAtct                                                                                                                                                 <  2:2094328/148‑1 (MQ=255)
       tGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCaa                                                                                                                                              <  2:1767796/148‑1 (MQ=255)
        gCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCaat                                                                                                                                             <  1:134489/148‑1 (MQ=255)
        gCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCaat                                                                                                                                             >  2:306728/1‑148 (MQ=255)
        gCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCaat                                                                                                                                             >  2:1563566/1‑148 (MQ=255)
         ccGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaata                                                                                                                                            <  1:3203439/148‑1 (MQ=255)
         ccGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCaata                                                                                                                                            <  2:2225858/148‑1 (MQ=255)
           ggAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataaa                                                                                                                                          <  1:122629/148‑1 (MQ=255)
           ggAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataaa                                                                                                                                          >  2:1545370/1‑148 (MQ=255)
            gagaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCaataaat                                                                                                                                         <  1:3043397/148‑1 (MQ=255)
            gagaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCaataaat                                                                                                                                         <  1:1267334/148‑1 (MQ=255)
             agaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataaata                                                                                                                                        >  2:2057453/1‑148 (MQ=255)
               aTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatg                                                                                                                                      <  1:254147/148‑1 (MQ=255)
               aTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatg                                                                                                                                      <  2:1830920/148‑1 (MQ=255)
               aTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATatg                                                                                                                                      >  2:615904/1‑148 (MQ=255)
                  cgcgCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatgatg                                                                                                                                   <  1:669904/148‑1 (MQ=255)
                   gcgcCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATgc                                                                                                                                  <  2:3322766/148‑1 (MQ=255)
                       catcatCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcgcgc                                                                                                                              <  1:1750652/148‑1 (MQ=255)
                         tcatcTAGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCAt                                                                                                                            >  2:1041650/1‑148 (MQ=255)
                          catcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCAtt                                                                                                                           <  1:503499/148‑1 (MQ=255)
                           atcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAACACCCGACACTGGCTCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTg                                                                                                                          <  2:1630270/148‑1 (MQ=255)
                            tcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTgc                                                                                                                         >  2:703798/1‑148 (MQ=255)
                             cTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTgcg                                                                                                                        >  1:7034/1‑148 (MQ=255)
                                gCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTgcggcg                                                                                                                     <  1:2706474/148‑1 (MQ=255)
                                    aGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCgattg                                                                                                                 <  1:1287725/148‑1 (MQ=255)
                                      tGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCgattgat                                                                                                               <  2:2996611/148‑1 (MQ=255)
                                       gAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattgatt                                                                                                              <  1:1123511/148‑1 (MQ=255)
                                          ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                           <  2:1687011/148‑1 (MQ=255)
                                          ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                           >  2:888675/1‑148 (MQ=255)
                                          ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                           <  2:177309/148‑1 (MQ=255)
                                           gCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTCACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTacac                                                                                                          >  1:263664/1‑148 (MQ=255)
                                           gCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTacac                                                                                                          >  2:2482227/1‑148 (MQ=255)
                                           gCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTacac                                                                                                          >  1:1984578/1‑148 (MQ=255)
                                            cTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAcc                                                                                                         >  2:3510425/1‑148 (MQ=255)
                                             ttttGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAccc                                                                                                        <  1:1555496/148‑1 (MQ=255)
                                                 gCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGc                                                                                                    <  1:3276976/148‑1 (MQ=255)
                                                 gCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGc                                                                                                    <  1:3275323/148‑1 (MQ=255)
                                                   cAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGccc                                                                                                  >  2:3146216/1‑148 (MQ=255)
                                                      gATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACg                                                                                               >  2:1133591/1‑148 (MQ=255)
                                                         atCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGTGGCGATTGATTACACCCTCGCCCACGATg                                                                                            <  1:505048/148‑1 (MQ=255)
                                                         atCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGTGGCGATTGATTACACCCTCGCCCACGATg                                                                                            <  1:527492/148‑1 (MQ=255)
                                                          tCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGa                                                                                           <  2:2176236/148‑1 (MQ=255)
                                                             ttCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACgg                                                                                        >  2:3354388/1‑148 (MQ=255)
                                                                aGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCTATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAt                                                                                     <  1:3121696/148‑1 (MQ=255)
                                                                 ggCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAtt                                                                                    >  1:2600604/1‑148 (MQ=255)
                                                                  gcgcTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                   >  2:2215682/1‑148 (MQ=255)
                                                                  gcgcTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                   >  2:1087517/1‑148 (MQ=255)
                                                                  gcgcTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                   >  2:2867551/1‑148 (MQ=255)
                                                                  gcgcTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                   <  2:445172/148‑1 (MQ=255)
                                                                  gcgcTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                   <  2:445222/148‑1 (MQ=255)
                                                                       ggTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTg                                                                              >  2:1865009/1‑148 (MQ=255)
                                                                       ggTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTg                                                                              >  2:58938/1‑148 (MQ=255)
                                                                              ccGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATc                                                                       >  1:673794/1‑148 (MQ=255)
                                                                               cGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGAACCCATGGCCTTAACCCTCATTATCTCAATAAATATGATGAGCGCATTGAGGCGATAGAATACACACTCGACCACGATGACGGCATTTCGTAGCGCAATCt                                                                      >  2:830457/1‑148 (MQ=255)
                                                                                gCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTg                                                                     >  2:928890/1‑148 (MQ=255)
                                                                                 cTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTgg                                                                    <  1:1665383/148‑1 (MQ=255)
                                                                                   acaacaAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAc                                                                  <  1:2129917/148‑1 (MQ=255)
                                                                                     aacaaGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCa                                                                >  1:3173080/1‑148 (MQ=255)
                                                                                      acaaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAg                                                               >  2:2970158/1‑148 (MQ=255)
                                                                                       caaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAgg                                                              <  1:2004656/148‑1 (MQ=255)
                                                                                        aaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGc                                                             <  1:1037398/148‑1 (MQ=255)
                                                                                        aaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGc                                                             <  1:1036979/148‑1 (MQ=255)
                                                                                             aTGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAgg                                                        <  2:2754375/148‑1 (MQ=255)
                                                                                              tGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGt                                                       <  1:2343199/148‑1 (MQ=255)
                                                                                               gAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTg                                                      >  2:1563240/1‑148 (MQ=255)
                                                                                                  aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATc                                                   <  2:2713249/148‑1 (MQ=255)
                                                                                                   cTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCTCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATcc                                                  <  1:2057460/148‑1 (MQ=255)
                                                                                                   cTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATcc                                                  <  1:2715017/148‑1 (MQ=255)
                                                                                                    tGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                 >  2:2720267/1‑148 (MQ=255)
                                                                                                    tGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                 >  2:2838829/1‑148 (MQ=255)
                                                                                                     ggATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTg                                                <  1:1099226/148‑1 (MQ=255)
                                                                                                         ccAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCg                                            >  1:2853495/1‑148 (MQ=255)
                                                                                                          cAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCgg                                           <  2:3497629/148‑1 (MQ=255)
                                                                                                           aaCGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGgt                                          >  1:2980881/1‑148 (MQ=255)
                                                                                                            aCGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGgtg                                         >  2:2895104/1‑148 (MQ=255)
                                                                                                             cGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGgtgt                                        >  2:1071812/1‑148 (MQ=255)
                                                                                                             cGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGgtgt                                        <  1:651868/148‑1 (MQ=255)
                                                                                                                   ttGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCg                                  <  1:473779/148‑1 (MQ=255)
                                                                                                                   ttGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCg                                  >  1:2764533/1‑148 (MQ=255)
                                                                                                                      cTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCACGGTGTTTCTCGCtg                               >  1:511462/1‑148 (MQ=255)
                                                                                                                        cATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCtgtg                             <  1:3509607/148‑1 (MQ=255)
                                                                                                                             tACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTaaa                        <  1:3222846/148‑1 (MQ=255)
                                                                                                                             tACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTaaa                        >  1:3418245/1‑148 (MQ=255)
                                                                                                                                ccATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAcc                     <  1:1545370/148‑1 (MQ=255)
                                                                                                                                        ttAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAg             <  2:3403996/148‑1 (MQ=255)
                                                                                                                                         tAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGt            <  1:1452320/148‑1 (MQ=255)
                                                                                                                                         tAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGt            >  1:2822295/1‑148 (MQ=255)
                                                                                                                                            cccTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTg         >  2:1494636/1‑148 (MQ=255)
                                                                                                                                            cccTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTg         <  1:888104/148‑1 (MQ=255)
                                                                                                                                            cccTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTg         >  1:824707/1‑148 (MQ=255)
                                                                                                                                               taataaTCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAt      >  1:919882/1‑148 (MQ=255)
                                                                                                                                                aattatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc     <  2:2217815/148‑1 (MQ=255)
                                                                                                                                                aatcatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc     >  1:2528170/1‑148 (MQ=255)
                                                                                                                                                aatcatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc     >  1:1406214/1‑148 (MQ=255)
                                                                                                                                                 ataatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCt    <  1:703798/148‑1 (MQ=255)
                                                                                                                                                   aatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTgg  >  2:2897120/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG  >  CP009273/1781918‑1782212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGCA  >  CP009273/1781916‑1782214
                                                                                                                                                     |                                                                                                                                                     
CCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATT                                                                                                                                                       <  SRR19973066.2002441/150‑1 (MQ=60)
CCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATT                                                                                                                                                       <  SRR19973066.2768767/150‑1 (MQ=60)
 CATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCTTACCCATGGCCTGAACCCTAATAA                                                                                                                                                      >  SRR19973066.343220/1‑150 (MQ=60)
  ATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTAT                                                                                                                                                     >  SRR19973066.2936280/1‑150 (MQ=60)
  ATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAAT                                                                                                                                                     <  SRR19973066.2468137/150‑1 (MQ=60)
  ATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAAT                                                                                                                                                     <  SRR19973066.1996903/150‑1 (MQ=60)
   TCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATC                                                                                                                                                    <  SRR19973066.459729/150‑1 (MQ=60)
    CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCT                                                                                                                                                   <  SRR19973066.42674/150‑1 (MQ=60)
     GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTC                                                                                                                                                  <  SRR19973066.677592/150‑1 (MQ=60)
      TGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCA                                                                                                                                                 >  SRR19973066.2820457/1‑150 (MQ=60)
          GCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAA                                                                                                                                             <  SRR19973066.135802/150‑1 (MQ=60)
           CCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAA                                                                                                                                            <  SRR19973066.3224697/150‑1 (MQ=60)
             GGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATA                                                                                                                                          <  SRR19973066.890140/150‑1 (MQ=60)
             GGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATA                                                                                                                                          <  SRR19973066.123864/150‑1 (MQ=60)
              GAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATAT                                                                                                                                         <  SRR19973066.3063740/150‑1 (MQ=60)
              GAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATAT                                                                                                                                         <  SRR19973066.1276486/150‑1 (MQ=60)
                 ATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGAT                                                                                                                                      <  SRR19973066.2306241/150‑1 (MQ=60)
                 ATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGAT                                                                                                                                      <  SRR19973066.256080/150‑1 (MQ=60)
                    CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                   <  SRR19973066.260790/150‑1 (MQ=60)
                    CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                   <  SRR19973066.674793/150‑1 (MQ=60)
                    CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                   <  SRR19973066.674732/150‑1 (MQ=60)
                     GCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGC                                                                                                                                  >  SRR19973066.1250360/1‑150 (MQ=60)
                      CGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCG                                                                                                                                 >  SRR19973066.252825/1‑150 (MQ=60)
                         CATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCAT                                                                                                                              <  SRR19973066.1763247/150‑1 (MQ=60)
                            CATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGC                                                                                                                           <  SRR19973066.507248/150‑1 (MQ=60)
                            CATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGC                                                                                                                           <  SRR19973066.507239/150‑1 (MQ=60)
                             ATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCG                                                                                                                          >  SRR19973066.7151/1‑150 (MQ=60)
                                  GCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGAT                                                                                                                     <  SRR19973066.2724624/150‑1 (MQ=60)
                                   CAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATT                                                                                                                    <  SRR19973066.681417/150‑1 (MQ=60)
                                      AGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGAT                                                                                                                 >  SRR19973066.3032214/1‑150 (MQ=60)
                                      AGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGAT                                                                                                                 >  SRR19973066.1451513/1‑150 (MQ=60)
                                      AGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGAT                                                                                                                 <  SRR19973066.1297035/150‑1 (MQ=60)
                                         GAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTAC                                                                                                              <  SRR19973066.1131602/150‑1 (MQ=60)
                                         GAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTAC                                                                                                              <  SRR19973066.1131584/150‑1 (MQ=60)
                                           AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTCACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                            >  SRR19973066.265640/1‑150 (MQ=60)
                                           AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTACTTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                            >  SRR19973066.1323580/1‑150 (MQ=60)
                                           AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                            >  SRR19973066.708852/1‑150 (MQ=60)
                                           AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                            >  SRR19973066.1998702/1‑150 (MQ=60)
                                           AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                            >  SRR19973066.1998571/1‑150 (MQ=60)
                                            GGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACC                                                                                                           <  SRR19973066.508969/150‑1 (MQ=60)
                                               TTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTC                                                                                                        <  SRR19973066.1565773/150‑1 (MQ=60)
                                               TTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTC                                                                                                        <  SRR19973066.1566680/150‑1 (MQ=60)
                                                   GCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCC                                                                                                    <  SRR19973066.3296814/150‑1 (MQ=60)
                                                   GCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCC                                                                                                    <  SRR19973066.3296842/150‑1 (MQ=60)
                                                   GCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCC                                                                                                    <  SRR19973066.3298489/150‑1 (MQ=60)
                                                        GATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGAT                                                                                               >  SRR19973066.2731472/1‑150 (MQ=60)
                                                           ATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGTGGCGATTGATTACACCCTCGCCCACGATGAC                                                                                            <  SRR19973066.508803/150‑1 (MQ=60)
                                                           ATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGTGGCGATTGATTACACCCTCGCCCACGATGAC                                                                                            <  SRR19973066.531356/150‑1 (MQ=60)
                                                                 CAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATT                                                                                      >  SRR19973066.2618204/1‑150 (MQ=60)
                                                                  AGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCTATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTT                                                                                     <  SRR19973066.3142542/150‑1 (MQ=60)
                                                                       CTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTG                                                                                <  SRR19973066.3227711/150‑1 (MQ=60)
                                                                          GTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGC                                                                             >  SRR19973066.1357936/1‑150 (MQ=60)
                                                                          GTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGC                                                                             >  SRR19973066.1046782/1‑150 (MQ=60)
                                                                          GTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGC                                                                             >  SRR19973066.3424267/1‑150 (MQ=60)
                                                                              CCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATC                                                                         >  SRR19973066.678728/1‑150 (MQ=60)
                                                                              CCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATC                                                                         <  SRR19973066.3167200/150‑1 (MQ=60)
                                                                                   CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                    <  SRR19973066.1677516/150‑1 (MQ=60)
                                                                                   CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                    <  SRR19973066.1677589/150‑1 (MQ=60)
                                                                                     ACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCA                                                                  <  SRR19973066.2144676/150‑1 (MQ=60)
                                                                                     ACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCA                                                                  >  SRR19973066.646711/1‑150 (MQ=60)
                                                                                     ACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCA                                                                  >  SRR19973066.3194209/1‑150 (MQ=60)
                                                                                      CAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAG                                                                 >  SRR19973066.2898280/1‑150 (MQ=60)
                                                                                         CAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCT                                                              <  SRR19973066.2018725/150‑1 (MQ=60)
                                                                                          AAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTC                                                             <  SRR19973066.1045031/150‑1 (MQ=60)
                                                                                          AAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTC                                                             <  SRR19973066.1044608/150‑1 (MQ=60)
                                                                                              GATGAAACTGGATCCAACGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGT                                                         >  SRR19973066.2972561/1‑150 (MQ=60)
                                                                                                TGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGA                                                       <  SRR19973066.2359151/150‑1 (MQ=60)
                                                                                                     CTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCTCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTG                                                  <  SRR19973066.2071734/150‑1 (MQ=60)
                                                                                                     CTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTG                                                  <  SRR19973066.2071727/150‑1 (MQ=60)
                                                                                                     CTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTG                                                  <  SRR19973066.2733253/150‑1 (MQ=60)
                                                                                                      TGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGC                                                 >  SRR19973066.935601/1‑150 (MQ=60)
                                                                                                       GGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCAGTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCT                                                <  SRR19973066.1107217/150‑1 (MQ=60)
                                                                                                         ATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCG                                              >  SRR19973066.2872671/1‑150 (MQ=60)
                                                                                                           CCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGT                                            >  SRR19973066.3000565/1‑150 (MQ=60)
                                                                                                           CCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGT                                            >  SRR19973066.1526374/1‑150 (MQ=60)
                                                                                                               CGCCGATTGCTCATAGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTT                                        <  SRR19973066.656612/150‑1 (MQ=60)
                                                                                                                   GATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCG                                    >  SRR19973066.2783155/1‑150 (MQ=60)
                                                                                                                     TTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCT                                  <  SRR19973066.477364/150‑1 (MQ=60)
                                                                                                                     TTGCACATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCT                                  <  SRR19973066.3123053/150‑1 (MQ=60)
                                                                                                                      TGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCACGGTGTTTCTCGCTG                                 >  SRR19973066.515245/1‑150 (MQ=60)
                                                                                                                       GCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGT                                <  SRR19973066.1141717/150‑1 (MQ=60)
                                                                                                                        CTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTG                               <  SRR19973066.500275/150‑1 (MQ=60)
                                                                                                                         TCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGG                              >  SRR19973066.2362820/1‑150 (MQ=60)
                                                                                                                         TCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGG                              <  SRR19973066.2674548/150‑1 (MQ=60)
                                                                                                                          CATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGT                             <  SRR19973066.3532990/150‑1 (MQ=60)
                                                                                                                             CGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAA                          >  SRR19973066.3441182/1‑150 (MQ=60)
                                                                                                                             CGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAA                          >  SRR19973066.3441111/1‑150 (MQ=60)
                                                                                                                              GTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAA                         >  SRR19973066.2767585/1‑150 (MQ=60)
                                                                                                                               TACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAC                        <  SRR19973066.3244162/150‑1 (MQ=60)
                                                                                                                                  CCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCC                     <  SRR19973066.1556427/150‑1 (MQ=60)
                                                                                                                                         CTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGT              >  SRR19973066.2841263/1‑150 (MQ=60)
                                                                                                                                           TAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCT            <  SRR19973066.3273539/150‑1 (MQ=60)
                                                                                                                                           TAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCT            <  SRR19973066.1462623/150‑1 (MQ=60)
                                                                                                                                            AACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTG           >  SRR19973066.830723/1‑150 (MQ=60)
                                                                                                                                              CCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTA         <  SRR19973066.894403/150‑1 (MQ=60)
                                                                                                                                              CCCTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTA         <  SRR19973066.894980/150‑1 (MQ=60)
                                                                                                                                               CCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAT        >  SRR19973066.926496/1‑150 (MQ=60)
                                                                                                                                                CTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC       >  SRR19973066.1416327/1‑150 (MQ=60)
                                                                                                                                                CTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC       >  SRR19973066.2545361/1‑150 (MQ=60)
                                                                                                                                                CTAATCATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC       >  SRR19973066.2546001/1‑150 (MQ=60)
                                                                                                                                                 TAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCT      >  SRR19973066.3075476/1‑150 (MQ=60)
                                                                                                                                                   ATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG    >  SRR19973066.1652051/1‑150 (MQ=60)
                                                                                                                                                   ATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG    >  SRR19973066.3222173/1‑150 (MQ=60)
                                                                                                                                                   ATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG    >  SRR19973066.2139385/1‑150 (MQ=60)
                                                                                                                                                   ATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG    >  SRR19973066.2141179/1‑150 (MQ=60)
                                                                                                                                                   ATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG    >  SRR19973066.3483544/1‑150 (MQ=60)
                                                                                                                                                   ATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGG    <  SRR19973066.708994/150‑1 (MQ=60)
                                                                                                                                                     TATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGCA  >  SRR19973066.1419104/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGCA  >  CP009273/1781916‑1782214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑