Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,782,065 A→T 93.3% N122Y (AAT→TAT)  ppsR → bifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,782,0650AT93.3% 500.1 / 20.9 165N122Y (AAT→TAT) ppsRbifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (4/7);  new base T (68/86);  total (72/93)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 173 total aligned reads displayed.

CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC  >  CP009273/1781920‑1782209
                                                                                                                                                 |                                                                                                                                                
cGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTaattat                                                                                                                                                <  1:1900695/148‑1 (MQ=255)
 gtgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAAtc                                                                                                                                               <  1:233870/148‑1 (MQ=255)
  tgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTAtct                                                                                                                                              <  1:3123055/148‑1 (MQ=255)
   gtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTAtctc                                                                                                                                             >  1:3260864/1‑148 (MQ=255)
    ttGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCa                                                                                                                                            <  2:884759/148‑1 (MQ=255)
    ttGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCa                                                                                                                                            <  1:2100010/148‑1 (MQ=255)
    ttGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCa                                                                                                                                            <  1:1049872/148‑1 (MQ=255)
     tGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaa                                                                                                                                           <  1:2920501/148‑1 (MQ=255)
       ccGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaata                                                                                                                                         <  2:1160982/148‑1 (MQ=255)
        cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataa                                                                                                                                        <  1:751956/148‑1 (MQ=255)
        cGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataa                                                                                                                                        <  2:3227122/148‑1 (MQ=255)
          gagaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataaat                                                                                                                                      <  2:148543/148‑1 (MQ=255)
           agaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataaata                                                                                                                                     >  1:3119155/1‑148 (MQ=255)
            gaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAatat                                                                                                                                    >  1:1089684/1‑148 (MQ=255)
            gaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAatat                                                                                                                                    <  1:1829368/148‑1 (MQ=255)
            gaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAatat                                                                                                                                    <  1:52989/148‑1 (MQ=255)
               tCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatgat                                                                                                                                 <  2:1671820/148‑1 (MQ=255)
                cgcgCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatgatg                                                                                                                                <  1:2825564/148‑1 (MQ=255)
                cgcgCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatgatg                                                                                                                                <  1:1786740/148‑1 (MQ=255)
                cgcgCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatgatg                                                                                                                                <  1:2278870/148‑1 (MQ=255)
                  cgcCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcg                                                                                                                              <  1:46369/148‑1 (MQ=255)
                  cgcCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcg                                                                                                                              <  1:46395/148‑1 (MQ=255)
                     catcatCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcgcgc                                                                                                                           >  1:370852/1‑148 (MQ=255)
                     catcatCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcgcgc                                                                                                                           >  1:3474265/1‑148 (MQ=255)
                      atcatcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCa                                                                                                                          <  1:2997968/148‑1 (MQ=255)
                      atcatcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCa                                                                                                                          <  2:3425054/148‑1 (MQ=255)
                      atcatcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCa                                                                                                                          >  1:2881980/1‑148 (MQ=255)
                      atcatcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCa                                                                                                                          <  1:1771318/148‑1 (MQ=255)
                      atcatcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCa                                                                                                                          <  2:234513/148‑1 (MQ=255)
                         atcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTg                                                                                                                       <  2:1840682/148‑1 (MQ=255)
                          tcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTgc                                                                                                                      >  2:1557466/1‑148 (MQ=255)
                            ttGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTgcgg                                                                                                                    <  2:3057953/148‑1 (MQ=255)
                             tGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTG‑CCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTgcggcg                                                                                                                  <  1:2827161/148‑1 (MQ=255)
                              gCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTgcggcg                                                                                                                  <  2:2858643/148‑1 (MQ=255)
                               cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCga                                                                                                                 <  1:589449/148‑1 (MQ=255)
                               cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCga                                                                                                                 >  1:2781681/1‑148 (MQ=255)
                               cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCga                                                                                                                 <  1:1045123/148‑1 (MQ=255)
                                aaaGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgat                                                                                                                <  1:1766517/148‑1 (MQ=255)
                                  aGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattg                                                                                                              >  2:1458416/1‑148 (MQ=255)
                                   gTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCCTGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattga                                                                                                             <  2:1054623/148‑1 (MQ=255)
                                   gTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattga                                                                                                             <  1:2914599/148‑1 (MQ=255)
                                   gTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattga                                                                                                             <  1:867123/148‑1 (MQ=255)
                                     gAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattgatt                                                                                                           >  1:507552/1‑148 (MQ=255)
                                      aaGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTa                                                                                                          >  1:2604559/1‑148 (MQ=255)
                                      aaGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTa                                                                                                          >  1:3514348/1‑148 (MQ=255)
                                       aGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTac                                                                                                         <  2:408503/148‑1 (MQ=255)
                                       aGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTac                                                                                                         <  1:1425682/148‑1 (MQ=255)
                                        ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                        >  2:822524/1‑148 (MQ=255)
                                        ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                        <  2:2321953/148‑1 (MQ=255)
                                        ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                        >  1:439123/1‑148 (MQ=255)
                                        ggCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                        >  1:1552058/1‑148 (MQ=255)
                                         gCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTacac                                                                                                       <  1:3369790/148‑1 (MQ=255)
                                         gCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTacac                                                                                                       <  1:3369591/148‑1 (MQ=255)
                                                ccAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGcc                                                                                                <  1:194928/148‑1 (MQ=255)
                                                  aGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCa                                                                                              >  2:1930208/1‑148 (MQ=255)
                                                  aGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCa                                                                                              <  2:1567772/148‑1 (MQ=255)
                                                  aGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCa                                                                                              <  2:1567088/148‑1 (MQ=255)
                                                  aGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCa                                                                                              >  1:1831458/1‑148 (MQ=255)
                                                   ggATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCAc                                                                                             >  2:933316/1‑148 (MQ=255)
                                                   ggATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCAc                                                                                             >  2:930993/1‑148 (MQ=255)
                                                    gATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACg                                                                                            >  2:1582238/1‑148 (MQ=255)
                                                              aGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAt                                                                                  >  1:1661003/1‑148 (MQ=255)
                                                               ggCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAtt                                                                                 >  2:598422/1‑148 (MQ=255)
                                                                     ggTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTg                                                                           >  1:1135531/1‑148 (MQ=255)
                                                                            ccGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATc                                                                    <  1:3181233/148‑1 (MQ=255)
                                                                             cGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCt                                                                   <  1:1098433/148‑1 (MQ=255)
                                                                               cTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTgg                                                                 <  1:1299476/148‑1 (MQ=255)
                                                                                  caacaaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAcc                                                              >  2:1040692/1‑148 (MQ=255)
                                                                                     caaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAgg                                                           >  1:2673893/1‑148 (MQ=255)
                                                                                       agagATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCt                                                         <  2:12457/148‑1 (MQ=255)
                                                                                        gagaTGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTc                                                        <  1:2408980/148‑1 (MQ=255)
                                                                                        gagaTGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTc                                                        >  2:3174161/1‑148 (MQ=255)
                                                                                            tGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGt                                                    <  2:3328056/148‑1 (MQ=255)
                                                                                              aaaCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGa                                                  <  1:236349/148‑1 (MQ=255)
                                                                                               aaCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGAt                                                 <  1:1462803/148‑1 (MQ=255)
                                                                                               aaCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGAt                                                 <  1:2701651/148‑1 (MQ=255)
                                                                                               aaCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGAt                                                 <  1:946574/148‑1 (MQ=255)
                                                                                                aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATc                                                <  2:39600/148‑1 (MQ=255)
                                                                                                 cTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAAGATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATcc                                               >  2:3255992/1‑148 (MQ=255)
                                                                                                   ggATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTg                                             >  2:2004943/1‑148 (MQ=255)
                                                                                                   ggATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTg                                             <  1:1396673/148‑1 (MQ=255)
                                                                                                    gATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGc                                            >  1:796453/1‑148 (MQ=255)
                                                                                                      tCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTc                                          >  2:3249942/1‑148 (MQ=255)
                                                                                                              cGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTtc                                  <  1:555803/148‑1 (MQ=255)
                                                                                                                 ttGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCg                               <  1:2321506/148‑1 (MQ=255)
                                                                                                                    cTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCtg                            <  1:1751708/148‑1 (MQ=255)
                                                                                                                       aTCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTgg                         <  2:2781681/148‑1 (MQ=255)
                                                                                                                           tACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTaaa                     <  2:3260864/148‑1 (MQ=255)
                                                                                                                           tACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTaaa                     <  2:71589/148‑1 (MQ=255)
                                                                                                                             cccATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAc                   <  2:1434249/148‑1 (MQ=255)
                                                                                                                              ccATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAcc                  >  2:1737849/1‑148 (MQ=255)
                                                                                                                              ccATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAcc                  >  2:1737870/1‑148 (MQ=255)
                                                                                                                                 gggCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAccccc               <  2:2732352/147‑1 (MQ=255)
                                                                                                                                         aCCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCt       >  1:655901/1‑148 (MQ=255)
                                                                                                                                           ccTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGt     <  1:2472843/148‑1 (MQ=255)
                                                                                                                                           ccTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGt     <  1:1688565/148‑1 (MQ=255)
                                                                                                                                              aattatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGTCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc  <  1:1638435/148‑1 (MQ=255)
                                                                                                                                              aattatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc  >  1:1240013/1‑148 (MQ=255)
                                                                                                                                              aattatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc  <  1:2055490/148‑1 (MQ=255)
                                                                                                                                              aataatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATc  <  1:342301/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC  >  CP009273/1781920‑1782209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGC  >  CP009273/1781919‑1782213
                                                                                                                                                  |                                                                                                                                                    
TCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATC                                                                                                                                                   <  SRR19973065.3490289/150‑1 (MQ=60)
 CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCT                                                                                                                                                  >  SRR19973065.2274988/1‑150 (MQ=60)
 CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCT                                                                                                                                                  <  SRR19973065.1913181/150‑1 (MQ=60)
  GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTC                                                                                                                                                 >  SRR19973065.3280605/1‑150 (MQ=60)
  GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTC                                                                                                                                                 <  SRR19973065.235564/150‑1 (MQ=60)
  GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTC                                                                                                                                                 <  SRR19973065.235617/150‑1 (MQ=60)
  GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTC                                                                                                                                                 <  SRR19973065.3024697/150‑1 (MQ=60)
   TGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCA                                                                                                                                                <  SRR19973065.3142075/150‑1 (MQ=60)
     TTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAAT                                                                                                                                              <  SRR19973065.2113516/150‑1 (MQ=60)
     TTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAAT                                                                                                                                              <  SRR19973065.135330/150‑1 (MQ=60)
     TTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAAT                                                                                                                                              <  SRR19973065.1056826/150‑1 (MQ=60)
      TGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATA                                                                                                                                             <  SRR19973065.2938250/150‑1 (MQ=60)
         CGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAAT                                                                                                                                          <  SRR19973065.756972/150‑1 (MQ=60)
         CGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAAT                                                                                                                                          <  SRR19973065.3236853/150‑1 (MQ=60)
          GGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATA                                                                                                                                         >  SRR19973065.3138162/1‑150 (MQ=60)
          GGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATA                                                                                                                                         >  SRR19973065.555911/1‑150 (MQ=60)
           GAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATAT                                                                                                                                        >  SRR19973065.1096862/1‑150 (MQ=60)
             GATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGA                                                                                                                                      <  SRR19973065.53573/150‑1 (MQ=60)
             GATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGA                                                                                                                                      <  SRR19973065.1841419/150‑1 (MQ=60)
              ATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGAT                                                                                                                                     >  SRR19973065.432050/1‑150 (MQ=60)
                 CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGANATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                  <  SRR19973065.1798019/150‑1 (MQ=60)
                 CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                  >  SRR19973065.2773861/1‑150 (MQ=60)
                 CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                  <  SRR19973065.2324825/150‑1 (MQ=60)
                 CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                  <  SRR19973065.1798436/150‑1 (MQ=60)
                 CGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                  <  SRR19973065.2843006/150‑1 (MQ=60)
                 CGCGCCATCATATTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTGTGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCG                                                                                                                                  <  SRR19973065.2293244/150‑1 (MQ=60)
                   CGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCG                                                                                                                                <  SRR19973065.46908/150‑1 (MQ=60)
                   CGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCG                                                                                                                                <  SRR19973065.46934/150‑1 (MQ=60)
                   CGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCG                                                                                                                                <  SRR19973065.46970/150‑1 (MQ=60)
                    GCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGC                                                                                                                               >  SRR19973065.3495403/1‑150 (MQ=60)
                    GCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGC                                                                                                                               >  SRR19973065.3495741/1‑150 (MQ=60)
                    GCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGC                                                                                                                               >  SRR19973065.373449/1‑150 (MQ=60)
                     CCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCA                                                                                                                              >  SRR19973065.2899616/1‑150 (MQ=60)
                     CCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCA                                                                                                                              >  SRR19973065.2899679/1‑150 (MQ=60)
                       ATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATT                                                                                                                            <  SRR19973065.1782915/150‑1 (MQ=60)
                       ATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATT                                                                                                                            <  SRR19973065.3016332/150‑1 (MQ=60)
                       ATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATT                                                                                                                            <  SRR19973065.1952542/150‑1 (MQ=60)
                          ATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCG                                                                                                                         <  SRR19973065.1744689/150‑1 (MQ=60)
                              TGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGA                                                                                                                     >  SRR19973065.2798939/1‑150 (MQ=60)
                              TGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTG‑CCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGAT                                                                                                                    <  SRR19973065.2844610/150‑1 (MQ=60)
                               GCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGAT                                                                                                                    <  SRR19973065.2844531/150‑1 (MQ=60)
                                CAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATT                                                                                                                   <  SRR19973065.1052030/150‑1 (MQ=60)
                                CAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATT                                                                                                                   <  SRR19973065.593105/150‑1 (MQ=60)
                                 AAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTG                                                                                                                  <  SRR19973065.1778154/150‑1 (MQ=60)
                                 AAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTG                                                                                                                  <  SRR19973065.1778102/150‑1 (MQ=60)
                                   AGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGAT                                                                                                                <  SRR19973065.1520256/150‑1 (MQ=60)
                                    GTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATT                                                                                                               <  SRR19973065.872687/150‑1 (MQ=60)
                                    GTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATT                                                                                                               <  SRR19973065.2932327/150‑1 (MQ=60)
                                    GTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATT                                                                                                               >  SRR19973065.510931/1‑150 (MQ=60)
                                     TGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTA                                                                                                              >  SRR19973065.3535653/1‑150 (MQ=60)
                                     TGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTA                                                                                                              <  SRR19973065.51700/150‑1 (MQ=60)
                                     TGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTA                                                                                                              >  SRR19973065.2620727/1‑150 (MQ=60)
                                       AAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACA                                                                                                            >  SRR19973065.442166/1‑150 (MQ=60)
                                       AAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACA                                                                                                            >  SRR19973065.1562341/1‑150 (MQ=60)
                                        AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                           <  SRR19973065.1434971/150‑1 (MQ=60)
                                          GCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCC                                                                                                         <  SRR19973065.3390200/150‑1 (MQ=60)
                                          GCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCC                                                                                                         <  SRR19973065.3390400/150‑1 (MQ=60)
                                                 CCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCA                                                                                                  <  SRR19973065.196446/150‑1 (MQ=60)
                                                 CCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCA                                                                                                  >  SRR19973065.1843523/1‑150 (MQ=60)
                                                        tctGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCAGTGCGGCGATTGATTACACCCTCGCCCACGATGAC                                                                                           <  SRR19973065.190419/147‑1 (MQ=60)
                                                        ATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGAC                                                                                           >  SRR19973065.3149279/1‑150 (MQ=60)
                                                        ATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGAC                                                                                           >  SRR19973065.1466104/1‑150 (MQ=60)
                                                        ATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGAC                                                                                           <  SRR19973065.785939/150‑1 (MQ=60)
                                                             TCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAT                                                                                      >  SRR19973065.1672130/1‑150 (MQ=60)
                                                                   GCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTT                                                                                >  SRR19973065.3291608/1‑150 (MQ=60)
                                                                    CTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTG                                                                               >  SRR19973065.2699022/1‑150 (MQ=60)
                                                                    CTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTG                                                                               >  SRR19973065.1142892/1‑150 (MQ=60)
                                                                      GGTTGCCCCTCTACAACAAGATATTAAACTGCATCAAACGCCGATTGCTCATCGTACCCATGGCCTTAACACTAATTATCTCAATAAATATGATGCGCTCAGTGCGGCGATTGATTACACCCTCGCCCACGATGAAGGCATTTCGTTGCG                                                                             <  SRR19973065.636398/150‑1 (MQ=60)
                                                                             CCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTG                                                                      <  SRR19973065.3200454/150‑1 (MQ=60)
                                                                              CGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGG                                                                     <  SRR19973065.1105660/150‑1 (MQ=60)
                                                                              CGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGG                                                                     <  SRR19973065.1105557/150‑1 (MQ=60)
                                                                                CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                   <  SRR19973065.1308037/150‑1 (MQ=60)
                                                                                    AACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGG                                                               >  SRR19973065.2690467/1‑150 (MQ=60)
                                                                                     ACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGC                                                              >  SRR19973065.3013136/1‑150 (MQ=60)
                                                                                         GAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAG                                                          <  SRR19973065.2424147/150‑1 (MQ=60)
                                                                                               AAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATC                                                    <  SRR19973065.238051/150‑1 (MQ=60)
                                                                                               AAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATC                                                    <  SRR19973065.238534/150‑1 (MQ=60)
                                                                                                AACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCC                                                   <  SRR19973065.952830/150‑1 (MQ=60)
                                                                                                AACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCC                                                   <  SRR19973065.2718443/150‑1 (MQ=60)
                                                                                                AACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCC                                                   <  SRR19973065.1472201/150‑1 (MQ=60)
                                                                                                   TGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGC                                                >  SRR19973065.801720/1‑150 (MQ=60)
                                                                                                    GGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCT                                               >  SRR19973065.2640124/1‑150 (MQ=60)
                                                                                                    GGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCT                                               <  SRR19973065.1405843/150‑1 (MQ=60)
                                                                                                     GATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTC                                              >  SRR19973065.1233171/1‑150 (MQ=60)
                                                                                                          AACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGT                                         >  SRR19973065.460833/1‑150 (MQ=60)
                                                                                                               CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                    <  SRR19973065.559355/150‑1 (MQ=60)
                                                                                                                  TTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCT                                 <  SRR19973065.2336054/150‑1 (MQ=60)
                                                                                                                    GCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGT                               <  SRR19973065.1084200/150‑1 (MQ=60)
                                                                                                                     CTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTG                              <  SRR19973065.2889237/150‑1 (MQ=60)
                                                                                                                     CTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTG                              <  SRR19973065.1763240/150‑1 (MQ=60)
                                                                                                                       CATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGT                            <  SRR19973065.698295/150‑1 (MQ=60)
                                                                                                                        ATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTA                           >  SRR19973065.180674/1‑150 (MQ=60)
                                                                                                                           GTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAA                        >  SRR19973065.3448347/1‑150 (MQ=60)
                                                                                                                                      CTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGT             >  SRR19973065.3427752/1‑150 (MQ=60)
                                                                                                                                      CTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGT             >  SRR19973065.1680244/1‑150 (MQ=60)
                                                                                                                                        TAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCT           >  SRR19973065.1564014/1‑150 (MQ=60)
                                                                                                                                        TAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCT           >  SRR19973065.660222/1‑150 (MQ=60)
                                                                                                                                            CCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAT       <  SRR19973065.2488410/150‑1 (MQ=60)
                                                                                                                                            CCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAT       <  SRR19973065.1699842/150‑1 (MQ=60)
                                                                                                                                            CCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAT       <  SRR19973065.2488401/150‑1 (MQ=60)
                                                                                                                                             CTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC      <  SRR19973065.936532/150‑1 (MQ=60)
                                                                                                                                             CTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC      >  SRR19973065.1248622/1‑150 (MQ=60)
                                                                                                                                             CTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGCTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATC      >  SRR19973065.1248160/1‑150 (MQ=60)
                                                                                                                                              TAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCT     <  SRR19973065.1005926/150‑1 (MQ=60)
                                                                                                                                               AATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGTCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG    <  SRR19973065.1649442/150‑1 (MQ=60)
                                                                                                                                               AATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG    <  SRR19973065.2068563/150‑1 (MQ=60)
                                                                                                                                               AATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG    >  SRR19973065.2901054/1‑150 (MQ=60)
                                                                                                                                               AATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG    <  SRR19973065.2068615/150‑1 (MQ=60)
                                                                                                                                               AATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG    <  SRR19973065.344692/150‑1 (MQ=60)
                                                                                                                                                 TAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGC  >  SRR19973065.2178556/1‑150 (MQ=60)
                                                                                                                                                 TAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGC  >  SRR19973065.2178530/1‑150 (MQ=60)
                                                                                                                                                 TAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGC  >  SRR19973065.2178479/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
TCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTGGC  >  CP009273/1781919‑1782213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑