Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,782,065	A→T	80.6%	N122Y (AAT→TAT)	ppsR →	bifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,782,065	0	A	T	80.6%	560.4 / 121.2	246	N122Y (AAT→TAT)	ppsR	bifunctional regulatory protein: PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand): ref base A (28/20); new base T (102/96); total (130/116)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

CATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG  >  CP009273/1781917‑1782211

   cGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAAtct                                                                                                                                                <  1:4197917/150‑1 (MQ=255)

    gtgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTAtctc                                                                                                                                               <  1:3638240/150‑1 (MQ=255)

    gtgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTAtctc                                                                                                                                               >  1:2025328/1‑150 (MQ=255)

    gtgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAAtctc                                                                                                                                               >  1:212470/1‑150 (MQ=255)

     tgtTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCa                                                                                                                                              >  2:3068765/1‑150 (MQ=255)

        tGCCGGAGATTCGCGCCATCATCTTACAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCaata                                                                                                                                           <  2:2074338/150‑1 (MQ=255)

         gCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATAGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGATCATCGTACCCATGGCCTTAACCCTAATTATCTCaataa                                                                                                                                          >  2:1482983/1‑150 (MQ=255)

            ggAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCaataaata                                                                                                                                       >  1:2989169/1‑150 (MQ=255)

              agaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAACTatg                                                                                                                                     >  2:15474/1‑150 (MQ=255)

              agaTTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATatg                                                                                                                                     <  1:4421135/150‑1 (MQ=255)

                 ttCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATatgatg                                                                                                                                  >  2:2283604/1‑150 (MQ=255)

                     cgcCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcgcg                                                                                                                              >  1:3331688/1‑150 (MQ=255)

                     cgacatcatCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATgcgcg                                                                                                                              <  2:493886/147‑1 (MQ=255)

                       ccATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCa                                                                                                                            >  1:3281396/1‑150 (MQ=255)

                          tcatcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTg                                                                                                                         <  2:4803781/150‑1 (MQ=255)

                           catcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTgc                                                                                                                        <  2:4008196/150‑1 (MQ=255)

                            atcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTgcg                                                                                                                       >  2:1265216/1‑150 (MQ=255)

                             tcTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTgcgg                                                                                                                      >  2:3009026/1‑150 (MQ=255)

                              cTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTgcggc                                                                                                                     >  2:3846524/1‑150 (MQ=255)

                                  cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTCCCCATGGCCTTAACCCTAATAATCTCAAGAAATATGATGCGCGCATTGCGGCgatt                                                                                                                 >  1:2218243/1‑150 (MQ=255)

                                  cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgatt                                                                                                                 >  2:1608677/1‑150 (MQ=255)

                                  cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCgatt                                                                                                                 >  2:713048/1‑150 (MQ=255)

                                  cAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCgatt                                                                                                                 <  1:3688502/150‑1 (MQ=255)

                                   aaaGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattg                                                                                                                <  2:2011472/150‑1 (MQ=255)

                                   aaaGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattg                                                                                                                <  2:2452795/150‑1 (MQ=255)

                                   aaaGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattg                                                                                                                <  1:1058038/150‑1 (MQ=255)

                                    aaGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCgattga                                                                                                               >  1:3293823/1‑150 (MQ=255)

                                     aGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattgat                                                                                                              <  2:4212178/150‑1 (MQ=255)

                                     aGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCgattgat                                                                                                              >  1:4836698/1‑150 (MQ=255)

                                       tGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTa                                                                                                            >  2:3266974/1‑150 (MQ=255)

                                       tGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTa                                                                                                            >  1:4104277/1‑150 (MQ=255)

                                         aaGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTaca                                                                                                          <  2:91074/150‑1 (MQ=255)

                                          aGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTacac                                                                                                         >  1:3844824/1‑150 (MQ=255)

                                            gCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAccc                                                                                                       >  2:195638/1‑150 (MQ=255)

                                             cTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCt                                                                                                      <  2:3171999/150‑1 (MQ=255)

                                             cTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCt                                                                                                      <  1:87269/150‑1 (MQ=255)

                                             cTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCt                                                                                                      <  2:1237165/150‑1 (MQ=255)

                                              ttttGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTc                                                                                                     <  1:1699496/150‑1 (MQ=255)

                                              ttttGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTc                                                                                                     <  1:1570175/150‑1 (MQ=255)

                                              ttttGCCAGGATATCGTTCAGGAGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTc                                                                                                     <  2:3894331/150‑1 (MQ=255)

                                               tttGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCg                                                                                                    >  2:3741716/1‑150 (MQ=255)

                                                 tGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGcc                                                                                                  <  2:2657539/150‑1 (MQ=255)

                                                 tGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGcc                                                                                                  <  2:4317525/150‑1 (MQ=255)

                                                  gCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGccc                                                                                                 <  2:4520708/150‑1 (MQ=255)

                                                  gCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGccc                                                                                                 <  2:2116825/150‑1 (MQ=255)

                                                    cAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCAc                                                                                               <  2:2711788/150‑1 (MQ=255)

                                                    cAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCAc                                                                                               >  1:2793101/1‑150 (MQ=255)

                                                     aGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACg                                                                                              >  2:3659446/1‑150 (MQ=255)

                                                        atatCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATg                                                                                           >  2:4696799/1‑150 (MQ=255)

                                                          atCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGAc                                                                                         <  2:738413/150‑1 (MQ=255)

                                                          atCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGAc                                                                                         <  2:3254835/150‑1 (MQ=255)

                                                          atCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGAc                                                                                         >  2:1549014/1‑150 (MQ=255)

                                                               tCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAt                                                                                    >  1:3977306/1‑150 (MQ=255)

                                                                 aGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                  >  2:37943/1‑150 (MQ=255)

                                                                 aGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAttt                                                                                  <  1:4023283/150‑1 (MQ=255)

                                                                     gcTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGtt                                                                              >  1:4503575/1‑150 (MQ=255)

                                                                      cTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTg                                                                             >  2:2188349/1‑150 (MQ=255)

                                                                      cTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTg                                                                             >  2:3707855/1‑150 (MQ=255)

                                                                      cTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTg                                                                             <  1:2943555/150‑1 (MQ=255)

                                                                        ggTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTgcg                                                                           <  1:5112369/150‑1 (MQ=255)

                                                                        ggTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTgcg                                                                           <  2:4134099/150‑1 (MQ=255)

                                                                        ggTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTgcg                                                                           >  1:4684356/1‑150 (MQ=255)

                                                                               ccGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTg                                                                    <  2:1329867/150‑1 (MQ=255)

                                                                                  cTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAc                                                                 <  1:4557455/150‑1 (MQ=255)

                                                                                       acaaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGc                                                            >  1:2376419/1‑150 (MQ=255)

                                                                                        caaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCt                                                           >  1:3144382/1‑150 (MQ=255)

                                                                                         aaGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTc                                                          <  1:4963895/150‑1 (MQ=255)

                                                                                               tGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGa                                                    >  2:417325/1‑150 (MQ=255)

                                                                                                 aaaCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATc                                                  <  1:1502643/150‑1 (MQ=255)

                                                                                                   aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                <  2:562056/150‑1 (MQ=255)

                                                                                                   aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                <  2:4606147/150‑1 (MQ=255)

                                                                                                   aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                >  1:1217492/1‑150 (MQ=255)

                                                                                                   aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                >  1:2291707/1‑150 (MQ=255)

                                                                                                   aCTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCt                                                >  1:3416595/1‑150 (MQ=255)

                                                                                                    cTGGATCCAACGCCGAGTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTg                                               <  1:1820859/150‑1 (MQ=255)

                                                                                                      ggATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCt                                             <  2:117979/150‑1 (MQ=255)

                                                                                                       gATCCAACGCCGATTACTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTc                                            <  2:1844801/150‑1 (MQ=255)

                                                                                                          ccAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGgt                                         >  1:1363295/1‑150 (MQ=255)

                                                                                                            aaCGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGgtgt                                       >  1:388926/1‑150 (MQ=255)

                                                                                                              cGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGttt                                     >  2:1400183/1‑150 (MQ=255)

                                                                                                               gCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTtc                                    >  1:3920743/1‑150 (MQ=255)

                                                                                                                 cGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTtctc                                  <  1:77492/150‑1 (MQ=255)

                                                                                                                 cGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTtctc                                  <  1:4588735/150‑1 (MQ=255)

                                                                                                                 cGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTtctc                                  <  1:509737/150‑1 (MQ=255)

                                                                                                                    ttGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCt                               <  1:4923971/150‑1 (MQ=255)

                                                                                                                    ttGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCt                               >  2:16932/1‑150 (MQ=255)

                                                                                                                    ttGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCt                               >  1:1843243/1‑150 (MQ=255)

                                                                                                                        tCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTgg                           >  2:3412688/1‑150 (MQ=255)

                                                                                                                                 ccATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAcccc                  >  2:3464981/1‑150 (MQ=255)

                                                                                                                                  cATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAccccc                 >  2:4660148/1‑150 (MQ=255)

                                                                                                                                   aTGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCcca                >  1:4597534/1‑150 (MQ=255)

                                                                                                                                   aTGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCcca                <  1:628734/150‑1 (MQ=255)

                                                                                                                                     ggCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACTGCATTTAGTTGCGAAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAACaccacc              >  1:2956989/1‑150 (MQ=255)

                                                                                                                                        cTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGt           >  1:2724233/1‑150 (MQ=255)

                                                                                                                                          tAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCt         >  1:505645/1‑150 (MQ=255)

                                                                                                                                            aCCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGt       <  1:56421/150‑1 (MQ=255)

                                                                                                                                            aCCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGt       <  2:2492658/150‑1 (MQ=255)

                                                                                                                                              ccTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAt     <  1:2512216/150‑1 (MQ=255)

                                                                                                                                              ccTAATAATCTCAATAACTATGATGCGCGCCTTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAGTTCGGTGCTCAATCTGGACCAGGCTCAGGTGATCCTGCTGGGGGTTTCTCGCTGGGGTAATACCCCCACCAGTcgggag     >  2:2951098/1‑145 (MQ=255)

                                                                                                                                                 aattatCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTg  <  1:953503/150‑1 (MQ=255)

CATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG  >  CP009273/1781917‑1782211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG  >  CP009273/1781920‑1782211

CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCT                                                                                                                                                <  SRR19973063.4207366/150‑1 (MQ=60)

CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCT                                                                                                                                                <  SRR19973063.4211315/150‑1 (MQ=60)

 GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTC                                                                                                                                               >  SRR19973063.2031650/1‑150 (MQ=60)

 GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTC                                                                                                                                               <  SRR19973063.3649914/150‑1 (MQ=60)

 GTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTC                                                                                                                                               >  SRR19973063.213109/1‑150 (MQ=60)

         GGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATA                                                                                                                                       >  SRR19973063.2998709/1‑150 (MQ=60)

          GAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATAT                                                                                                                                      >  SRR19973063.1815104/1‑150 (MQ=60)

           AGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATG                                                                                                                                     <  SRR19973063.4435330/150‑1 (MQ=60)

             ATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGAT                                                                                                                                   >  SRR19973063.4067617/1‑150 (MQ=60)

                  CGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCG                                                                                                                              >  SRR19973063.3342318/1‑150 (MQ=60)

                   GCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGC                                                                                                                             >  SRR19973063.4827721/1‑150 (MQ=60)

                   GCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTAAGGCGCTGGTAGCCCCGCTACAACAAGAGATGAAACTCTATCCAACGCCGAATGCTCATCGAACCCATGGCCTTAACCCAAATAATCTCAAGAAAAATGATGCGCGC                                                                                                                             >  SRR19973063.661341/1‑150 (MQ=60)

                    CCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCA                                                                                                                            >  SRR19973063.3291924/1‑150 (MQ=60)

                      ATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATT                                                                                                                          >  SRR19973063.4697337/1‑150 (MQ=60)

                      ATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATT                                                                                                                          <  SRR19973063.1788769/150‑1 (MQ=60)

                       TCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTG                                                                                                                         >  SRR19973063.4620881/1‑150 (MQ=60)

                        CATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGC                                                                                                                        >  SRR19973063.3083819/1‑150 (MQ=60)

                             TGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGA                                                                                                                   <  SRR19973063.799630/150‑1 (MQ=60)

                               CAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTCCCCATGGCCTTAACCCTAATAATCTCAAGAAATATGATGCGCGCATTGCGGCGATT                                                                                                                 >  SRR19973063.2225241/1‑150 (MQ=60)

                               CAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATT                                                                                                                 >  SRR19973063.235441/1‑150 (MQ=60)

                               CAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATT                                                                                                                 <  SRR19973063.3700311/150‑1 (MQ=60)

                                AAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTG                                                                                                                <  SRR19973063.1061244/150‑1 (MQ=60)

                                AAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTG                                                                                                                >  SRR19973063.204669/1‑150 (MQ=60)

                                 AAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGA                                                                                                               >  SRR19973063.1547206/1‑150 (MQ=60)

                                 AAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGA                                                                                                               >  SRR19973063.1547124/1‑150 (MQ=60)

                                 AAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGA                                                                                                               >  SRR19973063.3304262/1‑150 (MQ=60)

                                 AAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGA                                                                                                               >  SRR19973063.3304370/1‑150 (MQ=60)

                                  AGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGAT                                                                                                              >  SRR19973063.4852234/1‑150 (MQ=60)

                                   GTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATT                                                                                                             <  SRR19973063.3318058/150‑1 (MQ=60)

                                    TGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTA                                                                                                            <  SRR19973063.1400502/150‑1 (MQ=60)

                                    TGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTA                                                                                                            >  SRR19973063.4117426/1‑150 (MQ=60)

                                    TGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTA                                                                                                            >  SRR19973063.4117424/1‑150 (MQ=60)

                                     GAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTAC                                                                                                           >  SRR19973063.2297470/1‑150 (MQ=60)

                                       AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                         >  SRR19973063.3857047/1‑150 (MQ=60)

                                       AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                         >  SRR19973063.4018565/1‑150 (MQ=60)

                                       AGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACAC                                                                                                         >  SRR19973063.2484704/1‑150 (MQ=60)

                                        GGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACC                                                                                                        >  SRR19973063.980225/1‑150 (MQ=60)

                                          CTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCT                                                                                                      <  SRR19973063.87566/150‑1 (MQ=60)

                                           TTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTC                                                                                                     <  SRR19973063.1704676/150‑1 (MQ=60)

                                           TTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTC                                                                                                     <  SRR19973063.1574998/150‑1 (MQ=60)

                                            TTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCG                                                                                                    >  SRR19973063.2591725/1‑150 (MQ=60)

                                                CCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCA                                                                                                >  SRR19973063.2771681/1‑150 (MQ=60)

                                                 CAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCAC                                                                                               >  SRR19973063.2802085/1‑150 (MQ=60)

                                                            TCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAT                                                                                    >  SRR19973063.3990050/1‑150 (MQ=60)

                                                             CAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATT                                                                                   <  SRR19973063.4029644/150‑1 (MQ=60)

                                                              AGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTT                                                                                  <  SRR19973063.915957/150‑1 (MQ=60)

                                                              AGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTT                                                                                  <  SRR19973063.4036190/150‑1 (MQ=60)

                                                              AGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTT                                                                                  <  SRR19973063.5024449/150‑1 (MQ=60)

                                                               GGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCATCGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTC                                                                                 <  SRR19973063.2697987/150‑1 (MQ=60)

                                                               GGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTC                                                                                 <  SRR19973063.2960579/150‑1 (MQ=60)

                                                                GCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCG                                                                                >  SRR19973063.1267201/1‑150 (MQ=60)

                                                                 CGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGT                                                                               <  SRR19973063.5122879/150‑1 (MQ=60)

                                                                  GCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTT                                                                              >  SRR19973063.4518031/1‑150 (MQ=60)

                                                                  GCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTT                                                                              >  SRR19973063.4517938/1‑150 (MQ=60)

                                                                   CTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTG                                                                             <  SRR19973063.2952958/150‑1 (MQ=60)

                                                                    TGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGC                                                                            >  SRR19973063.2904485/1‑150 (MQ=60)

                                                                     GGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCG                                                                           <  SRR19973063.5128816/150‑1 (MQ=60)

                                                                     GGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCG                                                                           <  SRR19973063.5128793/150‑1 (MQ=60)

                                                                     GGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCG                                                                           >  SRR19973063.4699294/1‑150 (MQ=60)

                                                                         GCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAAT                                                                       <  SRR19973063.3481418/150‑1 (MQ=60)

                                                                         GCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAAT                                                                       <  SRR19973063.5062830/150‑1 (MQ=60)

                                                                           CCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCT                                                                     <  SRR19973063.5075790/150‑1 (MQ=60)

                                                                             CGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGG                                                                   <  SRR19973063.3788862/150‑1 (MQ=60)

                                                                             CGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGG                                                                   >  SRR19973063.2372124/1‑150 (MQ=60)

                                                                               CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                 <  SRR19973063.4572054/150‑1 (MQ=60)

                                                                               CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                 <  SRR19973063.446196/150‑1 (MQ=60)

                                                                               CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                 >  SRR19973063.3243596/1‑150 (MQ=60)

                                                                               CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                 <  SRR19973063.4572101/150‑1 (MQ=60)

                                                                               CTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGAC                                                                 <  SRR19973063.2974843/150‑1 (MQ=60)

                                                                                TACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACC                                                                <  SRR19973063.4578148/150‑1 (MQ=60)

                                                                                    ACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGC                                                            >  SRR19973063.2384031/1‑150 (MQ=60)

                                                                                     CAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCT                                                           >  SRR19973063.3154491/1‑150 (MQ=60)

                                                                                      AAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTC                                                          <  SRR19973063.4979945/150‑1 (MQ=60)

                                                                                      AAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTC                                                          <  SRR19973063.1140910/150‑1 (MQ=60)

                                                                                          GATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGT                                                      >  SRR19973063.1623688/1‑150 (MQ=60)

                                                                                             GAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGAT                                                   >  SRR19973063.604033/1‑150 (MQ=60)

                                                                                              AAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATC                                                  >  SRR19973063.2181755/1‑150 (MQ=60)

                                                                                              AAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATC                                                  >  SRR19973063.216642/1‑150 (MQ=60)

                                                                                              AAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATC                                                  <  SRR19973063.1507246/150‑1 (MQ=60)

                                                                                                ACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCT                                                >  SRR19973063.1221138/1‑150 (MQ=60)

                                                                                                ACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCT                                                >  SRR19973063.2299023/1‑150 (MQ=60)

                                                                                                ACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCT                                                >  SRR19973063.3427471/1‑150 (MQ=60)

                                                                                                ACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAAGAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGGTGCGCAATCTGGACCAGGCTCAGGTGATCCT                                                >  SRR19973063.834847/1‑150 (MQ=60)

                                                                                                 CTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTG                                               <  SRR19973063.1826725/150‑1 (MQ=60)

                                                                                                 CTGGATCCAACGCCGAGTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTG                                               <  SRR19973063.1826525/150‑1 (MQ=60)

                                                                                                  TGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTCTCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGC                                              >  SRR19973063.3662261/1‑150 (MQ=60)

                                                                                                      TCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGG                                          >  SRR19973063.337435/1‑150 (MQ=60)

                                                                                                       CCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGT                                         <  SRR19973063.3530075/150‑1 (MQ=60)

                                                                                                       CCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGT                                         >  SRR19973063.1367401/1‑150 (MQ=60)

                                                                                                       CCAACGCCGATTGCTCATCGTACACATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACACTCGCCCACGATGACGGCATTTCGTTTCGCAATATGGACCAGGCTCAGGTGATCCTGCTCGGT                                         >  SRR19973063.1367386/1‑150 (MQ=60)

                                                                                                         AACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGT                                       >  SRR19973063.390032/1‑150 (MQ=60)

                                                                                                           CGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTT                                     <  SRR19973063.875020/150‑1 (MQ=60)

                                                                                                            GCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTC                                    >  SRR19973063.3933251/1‑150 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  >  SRR19973063.4520116/1‑150 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  <  SRR19973063.77762/150‑1 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  <  SRR19973063.4399662/150‑1 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  <  SRR19973063.4603418/150‑1 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  <  SRR19973063.3903163/150‑1 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  <  SRR19973063.511218/150‑1 (MQ=60)

                                                                                                              CGATTGCTCATCGTACCCATGGCCTTAACCCTAATTATATCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCAGTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTC                                  >  SRR19973063.3215813/1‑150 (MQ=60)

                                                                                                                 TTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCT                               <  SRR19973063.4939870/150‑1 (MQ=60)

                                                                                                                 TTGCTCATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCT                               >  SRR19973063.3892234/1‑150 (MQ=60)

                                                                                                                 TTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCT                               >  SRR19973063.1848999/1‑150 (MQ=60)

                                                                                                                       ATCGTACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTA                         >  SRR19973063.3750812/1‑150 (MQ=60)

                                                                                                                          GTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAA                      <  SRR19973063.193686/150‑1 (MQ=60)

                                                                                                                            ACCCATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACC                    >  SRR19973063.4917472/1‑150 (MQ=60)

                                                                                                                               CATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCC                 >  SRR19973063.3946751/1‑150 (MQ=60)

                                                                                                                                ATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCA                <  SRR19973063.630585/150‑1 (MQ=60)

                                                                                                                                ATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCA                <  SRR19973063.1932898/150‑1 (MQ=60)

                                                                                                                                ATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCA                >  SRR19973063.4612208/1‑150 (MQ=60)

                                                                                                                                ATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCA                >  SRR19973063.4856562/1‑150 (MQ=60)

                                                                                                                                ATGGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCA                >  SRR19973063.4612246/1‑150 (MQ=60)

                                                                                                                                  GGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACTGCATTTAGTTGCGAAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAAACACCACC              >  SRR19973063.2966446/1‑150 (MQ=60)

                                                                                                                                  GGCCTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACC              <  SRR19973063.968365/150‑1 (MQ=60)

                                                                                                                                     CTTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGT           >  SRR19973063.2733026/1‑150 (MQ=60)

                                                                                                                                      TTAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTC          >  SRR19973063.284471/1‑150 (MQ=60)

                                                                                                                                       TAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCT         >  SRR19973063.604839/1‑150 (MQ=60)

                                                                                                                                       TAACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCT         >  SRR19973063.507111/1‑150 (MQ=60)

                                                                                                                                         ACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGT       >  SRR19973063.3333259/1‑150 (MQ=60)

                                                                                                                                         ACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGT       <  SRR19973063.56620/150‑1 (MQ=60)

                                                                                                                                         ACCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGT       <  SRR19973063.56598/150‑1 (MQ=60)

                                                                                                                                          CCCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTA      <  SRR19973063.3916445/150‑1 (MQ=60)

                                                                                                                                           CCTAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTAT     <  SRR19973063.2520225/150‑1 (MQ=60)

                                                                                                                                             TAATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCT   <  SRR19973063.2976436/150‑1 (MQ=60)

                                                                                                                                              AATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG  <  SRR19973063.956367/150‑1 (MQ=60)

                                                                                                                                              AATTATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG  >  SRR19973063.4967272/1‑150 (MQ=60)

CGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGCTTTTGCCAGGATATCGTTCAGGCGCTGGTTGCCCCGCTACAACAAGAGATGAAACTGGATCCAACGCCGATTGCTCATCGTACCCATGGCCTTAACCCTAATAATCTCAATAAATATGATGCGCGCATTGCGGCGATTGATTACACCCTCGCCCACGATGACGGCATTTCGTTGCGCAATCTGGACCAGGCTCAGGTGATCCTGCTCGGTGTTTCTCGCTGTGGTAAAACCCCCACCAGTCTGTATCTG  >  CP009273/1781920‑1782211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`