Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,128,128 T→A 100% K336* (AAA→TAA)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,128,1280TA95.8% 598.8 / 12.3 191K336* (AAA→TAA) wzccolanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base A (79/104);  total (84/107)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 191 total aligned reads displayed.

TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT  >  CP009273/2127983‑2128272
                                                                                                                                                 |                                                                                                                                                
 gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGgt                                                                                                                                               >  2:2995712/1‑148 (MQ=255)
 gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCAGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGgt                                                                                                                                               >  1:3219276/1‑148 (MQ=255)
    gACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTa                                                                                                                                            <  1:3238257/148‑1 (MQ=255)
       cAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAg                                                                                                                                         <  2:1051444/148‑1 (MQ=255)
       cAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAg                                                                                                                                         >  2:735545/1‑148 (MQ=255)
       cAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAg                                                                                                                                         >  2:2866537/1‑148 (MQ=255)
        aGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGc                                                                                                                                        <  2:435850/148‑1 (MQ=255)
        aGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGc                                                                                                                                        <  1:2325483/148‑1 (MQ=255)
         gACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGAACATCt                                                                                                                                       >  2:573576/1‑148 (MQ=255)
             cGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGga                                                                                                                                   <  1:1987434/148‑1 (MQ=255)
               aCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGgaga                                                                                                                                 <  2:870614/148‑1 (MQ=255)
               aCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGgaga                                                                                                                                 <  2:627428/148‑1 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                <  1:3003622/148‑1 (MQ=255)
                     cgGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCg                                                                                                                           <  2:3265364/148‑1 (MQ=255)
                      gggTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGc                                                                                                                          <  1:2277592/148‑1 (MQ=255)
                       ggTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGcc                                                                                                                         >  1:2111306/1‑148 (MQ=255)
                        gTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCct                                                                                                                        <  1:1698226/148‑1 (MQ=255)
                        gTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCct                                                                                                                        <  1:3463693/148‑1 (MQ=255)
                         tCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCctc                                                                                                                       >  1:1673989/1‑148 (MQ=255)
                         tCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTGGGAGATTTCCGCctc                                                                                                                       >  1:3020261/1‑148 (MQ=255)
                         tCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctc                                                                                                                       <  1:2789051/148‑1 (MQ=255)
                            gACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCgtt                                                                                                                    >  2:2919481/1‑148 (MQ=255)
                              cGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTaa                                                                                                                  >  2:3247066/1‑148 (MQ=255)
                              cGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTaa                                                                                                                  <  1:463188/148‑1 (MQ=255)
                              cGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTaa                                                                                                                  >  1:1860783/1‑148 (MQ=255)
                                gACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAg                                                                                                                <  1:502161/148‑1 (MQ=255)
                                 aCAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAgg                                                                                                               >  2:1227780/1‑148 (MQ=255)
                                 aCAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTATTCCACCGCCTGACGTTTATACAGCAGCGTGCGGTACGCCGGGTGTACTTAGGTGTACAGCTTGGAGATTTACGCCTCTTTAAAgg                                                                                                               >  1:616919/1‑148 (MQ=255)
                                   aaTCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTc                                                                                                             <  1:1129253/148‑1 (MQ=255)
                                   aaTCTCCTGCTGGGTTATCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTc                                                                                                             >  2:1805246/1‑148 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           >  1:744693/1‑148 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           >  2:3354620/1‑148 (MQ=255)
                                      ctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCACCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCGTGGAGATTTCCGCCGCGTTAAAGGTCAgt                                                                                                          >  1:920478/1‑148 (MQ=255)
                                       tcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgtt                                                                                                         <  2:1587095/148‑1 (MQ=255)
                                       tcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgtt                                                                                                         <  2:2449814/148‑1 (MQ=255)
                                           gctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgtt                                                                                                     >  2:1891748/1‑148 (MQ=255)
                                           gctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgtt                                                                                                     <  1:1063221/148‑1 (MQ=255)
                                            ctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgttc                                                                                                    >  1:3136939/1‑148 (MQ=255)
                                              gggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCaa                                                                                                  >  1:2623265/1‑148 (MQ=255)
                                               ggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAc                                                                                                 <  1:1071020/148‑1 (MQ=255)
                                                        cATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTCTTACAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCg                                                                                        >  1:1232828/1‑148 (MQ=255)
                                                         aTCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGa                                                                                       <  2:1777075/148‑1 (MQ=255)
                                                          tCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCACCGCCTGACGTTTATCCAGCAGCGTGCGGTAAGCCGGGTGAAAATATGTGTAAAGCGTGGAGATTTCCGCCTCTTTAAATGTAAGTTCGTTCAAATGCGCaaagat                                                                                      >  1:2143948/1‑143 (MQ=255)
                                                           cGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATg                                                                                     >  2:1624424/1‑148 (MQ=255)
                                                           cGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATg                                                                                     <  2:310086/148‑1 (MQ=255)
                                                             ccGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGtt                                                                                   <  1:735545/148‑1 (MQ=255)
                                                             ccGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGtt                                                                                   >  2:3044685/1‑148 (MQ=255)
                                                                      gACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcga                                                                          <  2:569249/148‑1 (MQ=255)
                                                                       aCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaa                                                                         >  1:2650524/1‑148 (MQ=255)
                                                                       aCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaa                                                                         >  1:863596/1‑148 (MQ=255)
                                                                        ccGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaat                                                                        <  2:1642367/148‑1 (MQ=255)
                                                                        ccGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaat                                                                        <  1:1507114/148‑1 (MQ=255)
                                                                          gTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCTGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatcg                                                                      <  2:321451/148‑1 (MQ=255)
                                                                           ttAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatcga                                                                     >  2:1539048/1‑148 (MQ=255)
                                                                             aaGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGc                                                                   <  1:971872/148‑1 (MQ=255)
                                                                              aGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCa                                                                  >  1:2733631/1‑148 (MQ=255)
                                                                                    gCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGc                                                              <  2:2668479/146‑1 (MQ=255)
                                                                                    gCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtt                                                            <  1:3331333/148‑1 (MQ=255)
                                                                                    gCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtt                                                            <  2:1645822/148‑1 (MQ=255)
                                                                                     cTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCttt                                                           <  1:268176/148‑1 (MQ=255)
                                                                                      tttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtttt                                                          >  2:1876865/1‑148 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         <  2:1243/148‑1 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         >  1:2861537/1‑148 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         <  2:604215/148‑1 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         >  1:699809/1‑148 (MQ=255)
                                                                                          tCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCtt                                                      <  1:2419680/148‑1 (MQ=255)
                                                                                                tCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCg                                                >  2:399544/1‑148 (MQ=255)
                                                                                                 ccAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCgg                                               <  2:1918693/148‑1 (MQ=255)
                                                                                                 ccAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCgg                                               >  2:2796131/1‑148 (MQ=255)
                                                                                                    gcgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAg                                            >  2:3540278/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           <  2:877/148‑1 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  1:3352240/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           <  1:356756/148‑1 (MQ=255)
                                                                                                      gcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGAt                                          <  2:704797/148‑1 (MQ=255)
                                                                                                        cTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCa                                        >  2:1470717/1‑148 (MQ=255)
                                                                                                         tGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                       <  1:1205392/148‑1 (MQ=255)
                                                                                                          gACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAc                                      >  2:1719576/1‑148 (MQ=255)
                                                                                                          gACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAc                                      >  1:1332148/1‑148 (MQ=255)
                                                                                                            cGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAg                                    >  2:1357722/1‑148 (MQ=255)
                                                                                                              tttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGaa                                  <  2:2179986/148‑1 (MQ=255)
                                                                                                                tctcCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATc                                <  1:1891748/148‑1 (MQ=255)
                                                                                                                 ctcCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCt                               <  2:62144/148‑1 (MQ=255)
                                                                                                                   ccAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCttt                             <  2:1710719/148‑1 (MQ=255)
                                                                                                                      gcagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATc                          <  2:1232742/148‑1 (MQ=255)
                                                                                                                      gcagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATc                          <  1:143379/148‑1 (MQ=255)
                                                                                                                       cagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATcc                         <  1:721015/148‑1 (MQ=255)
                                                                                                                       cagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATcc                         >  2:779996/1‑148 (MQ=255)
                                                                                                                        agcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCt                        <  2:690/148‑1 (MQ=255)
                                                                                                                        agcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCt                        <  2:657/148‑1 (MQ=255)
                                                                                                                          cGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGa                      <  1:3247066/148‑1 (MQ=255)
                                                                                                                            tGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACg                    >  2:464218/1‑148 (MQ=255)
                                                                                                                              cGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGa                  <  1:2866537/148‑1 (MQ=255)
                                                                                                                                 tACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAgg               >  2:1133405/1‑148 (MQ=255)
                                                                                                                                  aCGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGc              <  1:1447992/148‑1 (MQ=255)
                                                                                                                                    gCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCAt            >  2:2580026/1‑148 (MQ=255)
                                                                                                                                    gCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCGGACGGAAGGCAt            <  2:2237048/148‑1 (MQ=255)
                                                                                                                                         gTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  1:252728/148‑1 (MQ=255)
                                                                                                                                             aCTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgt   <  1:1229029/148‑1 (MQ=255)
                                                                                                                                              cTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgtt  >  1:1415041/1‑148 (MQ=255)
                                                                                                                                              cTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgtt  <  2:2111306/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT  >  CP009273/2127983‑2128272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCG  >  CP009273/2127979‑2128276
                                                                                                                                                     |                                                                                                                                                    
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTA                                                                                                                                                      <  SRR19973066.525065/150‑1 (MQ=60)
 ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                     <  SRR19973066.1023457/150‑1 (MQ=60)
 ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                     <  SRR19973066.3504475/150‑1 (MQ=60)
 ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                     <  SRR19973066.1730920/150‑1 (MQ=60)
 ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                     <  SRR19973066.3472523/150‑1 (MQ=60)
 ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                     <  SRR19973066.3472562/150‑1 (MQ=60)
  CCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGG                                                                                                                                                    <  SRR19973066.2359483/150‑1 (MQ=60)
   CTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGT                                                                                                                                                   >  SRR19973066.1456608/1‑150 (MQ=60)
   CTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGT                                                                                                                                                   <  SRR19973066.831516/150‑1 (MQ=60)
   CTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGT                                                                                                                                                   <  SRR19973066.1413082/150‑1 (MQ=60)
   CTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCAGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGT                                                                                                                                                   >  SRR19973066.3240579/1‑150 (MQ=60)
     GCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGT                                                                                                                                                 <  SRR19973066.3510859/150‑1 (MQ=60)
        GACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACA                                                                                                                                              <  SRR19973066.3259632/150‑1 (MQ=60)
        GACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACA                                                                                                                                              <  SRR19973066.3259613/150‑1 (MQ=60)
           CAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCT                                                                                                                                           <  SRR19973066.934502/150‑1 (MQ=60)
            AGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTT                                                                                                                                          <  SRR19973066.2341364/150‑1 (MQ=60)
             GACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTG                                                                                                                                         <  SRR19973066.1279524/150‑1 (MQ=60)
               CTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCGTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGA                                                                                                                                       >  SRR19973066.1517615/1‑150 (MQ=60)
                 CGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGA                                                                                                                                     >  SRR19973066.2891896/1‑150 (MQ=60)
                 CGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGA                                                                                                                                     <  SRR19973066.2001442/150‑1 (MQ=60)
                  GACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAT                                                                                                                                    <  SRR19973066.2741539/150‑1 (MQ=60)
                    CATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTT                                                                                                                                  <  SRR19973066.3023596/150‑1 (MQ=60)
                         CGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCC                                                                                                                             >  SRR19973066.2125888/1‑150 (MQ=60)
                          GGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCT                                                                                                                            <  SRR19973066.2293235/150‑1 (MQ=60)
                          GGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCT                                                                                                                            <  SRR19973066.2292911/150‑1 (MQ=60)
                           GGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTC                                                                                                                           >  SRR19973066.1686184/1‑150 (MQ=60)
                           GGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTGGGAGATTTCCGCCTC                                                                                                                           >  SRR19973066.3040406/1‑150 (MQ=60)
                            GTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCT                                                                                                                          <  SRR19973066.1710569/150‑1 (MQ=60)
                            GTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCT                                                                                                                          <  SRR19973066.3486847/150‑1 (MQ=60)
                             TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                         <  SRR19973066.2807822/150‑1 (MQ=60)
                             TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                         <  SRR19973066.2808928/150‑1 (MQ=60)
                             TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                         >  SRR19973066.3433251/1‑150 (MQ=60)
                                GNCGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAA                                                                                                                      >  SRR19973066.1874089/1‑150 (MQ=60)
                                  CGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAG                                                                                                                    <  SRR19973066.467759/150‑1 (MQ=60)
                                  CGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAG                                                                                                                    <  SRR19973066.466728/150‑1 (MQ=60)
                                   GGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGG                                                                                                                   >  SRR19973066.2293827/1‑150 (MQ=60)
                                   GGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTATTCCACCGCCTGACGTTTATACAGCAGCGTGCGGTACGCCGGGTGTACTTAGGTGTACAGCTTGGAGATTTACGCCTCTTTAAAGG                                                                                                                   >  SRR19973066.621260/1‑150 (MQ=60)
                                    GACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGT                                                                                                                  <  SRR19973066.505891/150‑1 (MQ=60)
                                       AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                               <  SRR19973066.1137363/150‑1 (MQ=60)
                                       AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                               >  SRR19973066.750221/1‑150 (MQ=60)
                                        ATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTATCCAGCAGCGTGCGGTACGCCGGGTGAACATAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGT                                                                                                              >  SRR19973066.927088/1‑150 (MQ=60)
                                        ATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCACCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCGTGGAGATTTCCGCCGCGTTAAAGGTCAGT                                                                                                              >  SRR19973066.927099/1‑150 (MQ=60)
                                         TCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTT                                                                                                             <  SRR19973066.1115594/150‑1 (MQ=60)
                                          CTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTC                                                                                                            >  SRR19973066.2537611/1‑150 (MQ=60)
                                           TCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCG                                                                                                           <  SRR19973066.1160708/150‑1 (MQ=60)
                                              TGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTC                                                                                                        >  SRR19973066.3157876/1‑150 (MQ=60)
                                               GCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCA                                                                                                       <  SRR19973066.1071014/150‑1 (MQ=60)
                                                CTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAA                                                                                                      >  SRR19973066.2640959/1‑150 (MQ=60)
                                                 TGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAC                                                                                                     >  SRR19973066.3284546/1‑150 (MQ=60)
                                                  GGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACT                                                                                                    <  SRR19973066.529172/150‑1 (MQ=60)
                                                   GGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTG                                                                                                   <  SRR19973066.1078872/150‑1 (MQ=60)
                                                     TTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCG                                                                                                 <  SRR19973066.1955831/150‑1 (MQ=60)
                                                       TTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCA                                                                                               <  SRR19973066.2143296/150‑1 (MQ=60)
                                                          GGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCGCTTTAAATGTCAGTTAGTTCAACTGCGCATCG                                                                                            >  SRR19973066.1241500/1‑150 (MQ=60)
                                                          GGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTCTTACAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCGCTTTAAAGGTCAGTTCGTTCAACTGCGCATCG                                                                                            >  SRR19973066.1241586/1‑150 (MQ=60)
                                                           GCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGA                                                                                           >  SRR19973066.3181559/1‑150 (MQ=60)
                                                            CATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCACCGCCTGACGTTTATCCAGCAGCGTGCGGTAAGCCGGGTGAAAATATGTGTAAAGCGTGGAGATTTCCGCCTCTTTAAATGTAAGTTCGTTCAAATGCGCAaagat                                                                                          >  SRR19973066.2158831/1‑145 (MQ=60)
                                                              TCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGT                                                                                        <  SRR19973066.1670918/150‑1 (MQ=60)
                                                                GCCGTTACGCGACCGTTAAGTTTGGCGTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTC                                                                                      >  SRR19973066.1390379/1‑150 (MQ=60)
                                                                 CCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCA                                                                                     <  SRR19973066.741003/150‑1 (MQ=60)
                                                                         CGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAA                                                                             >  SRR19973066.869803/1‑150 (MQ=60)
                                                                         CGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAA                                                                             >  SRR19973066.2668315/1‑150 (MQ=60)
                                                                           ACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATC                                                                           <  SRR19973066.2394360/150‑1 (MQ=60)
                                                                            CCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCG                                                                          <  SRR19973066.1517637/150‑1 (MQ=60)
                                                                              GTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAG                                                                        >  SRR19973066.865565/1‑150 (MQ=60)
                                                                               TTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGC                                                                       <  SRR19973066.392234/150‑1 (MQ=60)
                                                                                TAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCA                                                                      >  SRR19973066.2752016/1‑150 (MQ=60)
                                                                                 AAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAC                                                                     <  SRR19973066.979060/150‑1 (MQ=60)
                                                                                 AAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAC                                                                     >  SRR19973066.203993/1‑150 (MQ=60)
                                                                                 AAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAC                                                                     <  SRR19973066.979021/150‑1 (MQ=60)
                                                                                      TGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTT                                                                >  SRR19973066.2686328/1‑150 (MQ=60)
                                                                                        gcgctgTCGTCTGCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGGACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTT                                                              <  SRR19973066.3353484/144‑1 (MQ=60)
                                                                                        GCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTT                                                              <  SRR19973066.3353482/150‑1 (MQ=60)
                                                                                         CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                             >  SRR19973066.704970/1‑150 (MQ=60)
                                                                                         CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                             >  SRR19973066.2880751/1‑150 (MQ=60)
                                                                                         CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                             <  SRR19973066.270170/150‑1 (MQ=60)
                                                                                              TCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCC                                                        <  SRR19973066.2436097/150‑1 (MQ=60)
                                                                                                       AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                               >  SRR19973066.3374580/1‑150 (MQ=60)
                                                                                                         CGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATC                                             <  SRR19973066.359537/150‑1 (MQ=60)
                                                                                                            CTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAC                                          >  SRR19973066.1341819/1‑150 (MQ=60)
                                                                                                             TGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACA                                         <  SRR19973066.1213791/150‑1 (MQ=60)
                                                                                                              GACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAG                                        >  SRR19973066.2594031/1‑150 (MQ=60)
                                                                                                               ACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGA                                       <  SRR19973066.626402/150‑1 (MQ=60)
                                                                                                                    TCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTT                                  <  SRR19973066.1905235/150‑1 (MQ=60)
                                                                                                                        CAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATC                              >  SRR19973066.3047716/1‑150 (MQ=60)
                                                                                                                          GCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCT                            <  SRR19973066.144753/150‑1 (MQ=60)
                                                                                                                           CAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTG                           <  SRR19973066.726355/150‑1 (MQ=60)
                                                                                                                           CAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTG                           <  SRR19973066.726354/150‑1 (MQ=60)
                                                                                                                           CAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTG                           <  SRR19973066.726320/150‑1 (MQ=60)
                                                                                                                              CGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACG                        <  SRR19973066.3268469/150‑1 (MQ=60)
                                                                                                                              CGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGGTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACG                        <  SRR19973066.3035783/150‑1 (MQ=60)
                                                                                                                                  CGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAG                    <  SRR19973066.2885757/150‑1 (MQ=60)
                                                                                                                                  CGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAG                    <  SRR19973066.2885770/150‑1 (MQ=60)
                                                                                                                                      ACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCAT                <  SRR19973066.1458280/150‑1 (MQ=60)
                                                                                                                                            GGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTT          <  SRR19973066.1876169/150‑1 (MQ=60)
                                                                                                                                             GTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTT         <  SRR19973066.254657/150‑1 (MQ=60)
                                                                                                                                              TGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTG        <  SRR19973066.1838926/150‑1 (MQ=60)
                                                                                                                                               GAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGT       >  SRR19973066.2792000/1‑150 (MQ=60)
                                                                                                                                                AACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT      >  SRR19973066.1425803/1‑150 (MQ=60)
                                                                                                                                                AACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT      >  SRR19973066.1425184/1‑150 (MQ=60)
                                                                                                                                                 ACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTT     >  SRR19973066.1539604/1‑150 (MQ=60)
                                                                                                                                                 ACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTT     <  SRR19973066.1237755/150‑1 (MQ=60)
                                                                                                                                                 ACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTT     <  SRR19973066.1236488/150‑1 (MQ=60)
                                                                                                                                                 ACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTT     >  SRR19973066.972578/1‑150 (MQ=60)
                                                                                                                                                 ACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTT     >  SRR19973066.972639/1‑150 (MQ=60)
                                                                                                                                                   TTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTC   <  SRR19973066.460642/150‑1 (MQ=60)
                                                                                                                                                    TAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCG  <  SRR19973066.1595844/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCG  >  CP009273/2127979‑2128276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑