Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,128,128 T→A 100% K336* (AAA→TAA)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,128,1280TA99.0% 677.8 / ‑2.5 205K336* (AAA→TAA) wzccolanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (94/109);  total (94/111)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT  >  CP009273/2127983‑2128272
                                                                                                                                                 |                                                                                                                                                
tgctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAgg                                                                                                                                                <  1:2854789/148‑1 (MQ=255)
 gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGgt                                                                                                                                               >  2:2270314/1‑148 (MQ=255)
    gACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTa                                                                                                                                            >  1:813496/1‑148 (MQ=255)
     aCCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTAc                                                                                                                                           >  2:3736941/1‑148 (MQ=255)
       cAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAg                                                                                                                                         <  1:3545889/148‑1 (MQ=255)
        aGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGc                                                                                                                                        >  2:92772/1‑148 (MQ=255)
          aCTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCtt                                                                                                                                      <  1:1903117/148‑1 (MQ=255)
          aCTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCtt                                                                                                                                      >  2:2125785/1‑148 (MQ=255)
            tCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTgg                                                                                                                                    >  1:2603350/1‑148 (MQ=255)
            tCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTgg                                                                                                                                    >  1:2584328/1‑148 (MQ=255)
            tCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTgg                                                                                                                                    >  1:1433308/1‑148 (MQ=255)
            tCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTgg                                                                                                                                    >  1:672195/1‑148 (MQ=255)
            tCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTgg                                                                                                                                    <  1:442137/148‑1 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                >  2:1479239/1‑148 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                >  2:1012161/1‑148 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                >  1:2290048/1‑148 (MQ=255)
                  tCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAttt                                                                                                                              <  2:1288526/148‑1 (MQ=255)
                      gggTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGc                                                                                                                          <  1:3693802/148‑1 (MQ=255)
                         tCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctc                                                                                                                       <  1:2681084/148‑1 (MQ=255)
                         tCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctc                                                                                                                       <  1:2151578/148‑1 (MQ=255)
                         tCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctc                                                                                                                       <  1:763870/148‑1 (MQ=255)
                          cAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctct                                                                                                                      <  1:1502909/148‑1 (MQ=255)
                          cAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctct                                                                                                                      <  1:2798972/148‑1 (MQ=255)
                          cAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctct                                                                                                                      >  1:2032203/1‑148 (MQ=255)
                              cGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTaa                                                                                                                  <  2:3495920/148‑1 (MQ=255)
                                gACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACTTTTATCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTACGCCTCTTTAAAg                                                                                                                >  2:210014/1‑148 (MQ=255)
                                   aaTCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTc                                                                                                             <  2:2869278/148‑1 (MQ=255)
                                    aTCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCa                                                                                                            >  2:2815929/1‑148 (MQ=255)
                                    aTCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCa                                                                                                            >  2:1979017/1‑148 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           >  1:591304/1‑148 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           >  1:592502/1‑148 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           >  2:2500056/1‑148 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           >  1:3250283/1‑148 (MQ=255)
                                     tatcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           <  2:2296157/146‑1 (MQ=255)
                                      ctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgt                                                                                                          >  2:3263582/1‑148 (MQ=255)
                                       tcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgtt                                                                                                         <  2:2067999/148‑1 (MQ=255)
                                        ccTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttc                                                                                                        >  1:3813904/1‑148 (MQ=255)
                                        ccTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttc                                                                                                        >  1:2181888/1‑148 (MQ=255)
                                          tgctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgt                                                                                                      >  1:826835/1‑148 (MQ=255)
                                             tgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCa                                                                                                   <  1:567384/148‑1 (MQ=255)
                                              gggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCaa                                                                                                  <  2:1687678/148‑1 (MQ=255)
                                               ggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTCTTCCAGCGCCGGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCGTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAc                                                                                                 >  1:247358/1‑148 (MQ=255)
                                                    tCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTgcgc                                                                                            <  2:637960/148‑1 (MQ=255)
                                                    tCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTgcgc                                                                                            <  1:77776/148‑1 (MQ=255)
                                                        cATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCg                                                                                        >  2:388347/1‑148 (MQ=255)
                                                          tCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGAt                                                                                      >  2:227414/1‑148 (MQ=255)
                                                            gCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGt                                                                                    <  2:3662949/148‑1 (MQ=255)
                                                              cGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTc                                                                                  <  2:3504603/148‑1 (MQ=255)
                                                              cGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTc                                                                                  <  1:2807275/148‑1 (MQ=255)
                                                                  aCGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCa                                                                              >  1:1694324/1‑148 (MQ=255)
                                                                       aCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaa                                                                         >  1:1677565/1‑148 (MQ=255)
                                                                       aCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTAACCatctaa                                                                         >  2:1670898/1‑148 (MQ=255)
                                                                          gTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatcg                                                                      <  2:801419/148‑1 (MQ=255)
                                                                               gTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAc                                                                 >  2:716317/1‑148 (MQ=255)
                                                                                    gCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtt                                                            >  2:3302743/1‑148 (MQ=255)
                                                                                     cTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCttt                                                           <  1:2409405/148‑1 (MQ=255)
                                                                                      tttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtttt                                                          >  1:1116359/1‑148 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGTGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         >  1:1383104/1‑148 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         <  2:2390608/148‑1 (MQ=255)
                                                                                       ttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                         >  1:1383611/1‑148 (MQ=255)
                                                                                        tttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAGTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGc                                                        <  2:1187697/148‑1 (MQ=255)
                                                                                           cGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTc                                                     <  1:1268448/148‑1 (MQ=255)
                                                                                             tCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCa                                                   >  2:2761459/1‑148 (MQ=255)
                                                                                                  cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGc                                              <  2:3625263/148‑1 (MQ=255)
                                                                                                  cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGc                                              <  1:539014/148‑1 (MQ=255)
                                                                                                   aGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCa                                             >  2:1882889/1‑148 (MQ=255)
                                                                                                    gcgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAg                                            >  2:3535141/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  1:1867636/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  2:447703/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  2:3403554/1‑148 (MQ=255)
                                                                                                      gcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGAt                                          <  1:1033500/148‑1 (MQ=255)
                                                                                                       ccTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATc                                         >  1:1032043/1‑148 (MQ=255)
                                                                                                         tGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                       <  1:2515659/148‑1 (MQ=255)
                                                                                                         tGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                       <  2:3241290/148‑1 (MQ=255)
                                                                                                          gACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCCTCCAGCGGCAGATCAAc                                      >  2:1155690/1‑148 (MQ=255)
                                                                                                              tttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGaa                                  <  2:2032001/148‑1 (MQ=255)
                                                                                                               ttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAAt                                 >  2:2583923/1‑148 (MQ=255)
                                                                                                               ttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAAt                                 >  2:2462903/1‑148 (MQ=255)
                                                                                                                tctcCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATc                                <  1:2125785/148‑1 (MQ=255)
                                                                                                                tctcCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATc                                <  2:2233389/148‑1 (MQ=255)
                                                                                                                 ctcCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCt                               <  2:3711215/148‑1 (MQ=255)
                                                                                                                   ccAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCttt                             <  1:3111807/148‑1 (MQ=255)
                                                                                                                   ccAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCttt                             <  1:1979017/148‑1 (MQ=255)
                                                                                                                      gcagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATc                          <  2:2290048/148‑1 (MQ=255)
                                                                                                                         gcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTg                       <  2:1254133/148‑1 (MQ=255)
                                                                                                                          cGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGa                      <  1:716317/148‑1 (MQ=255)
                                                                                                                          cGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGa                      <  1:2270314/148‑1 (MQ=255)
                                                                                                                               ggTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGaa                 <  1:2761459/148‑1 (MQ=255)
                                                                                                                                 tACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAgg               >  1:3582027/1‑148 (MQ=255)
                                                                                                                                  aCGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGc              <  2:1505726/148‑1 (MQ=255)
                                                                                                                                  aCGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGc              <  2:3202326/148‑1 (MQ=255)
                                                                                                                                  aCGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGc              <  2:2907836/148‑1 (MQ=255)
                                                                                                                                   cGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCa             <  1:3431369/148‑1 (MQ=255)
                                                                                                                                        ggTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAg        >  2:1810398/1‑148 (MQ=255)
                                                                                                                                         gTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  2:2603350/148‑1 (MQ=255)
                                                                                                                                         gTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  2:1043141/148‑1 (MQ=255)
                                                                                                                                         gTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  2:672195/148‑1 (MQ=255)
                                                                                                                                         gTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  2:3147452/148‑1 (MQ=255)
                                                                                                                                          tGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtt      >  2:3021193/1‑148 (MQ=255)
                                                                                                                                             aCTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgt   >  2:3572140/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT  >  CP009273/2127983‑2128272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTT  >  CP009273/2127980‑2128274
                                                                                                                                                    |                                                                                                                                                  
ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                   <  SRR19973069.3645196/150‑1 (MQ=60)
ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                   <  SRR19973069.1245104/150‑1 (MQ=60)
ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                   <  SRR19973069.3161977/150‑1 (MQ=60)
ACCTGCTGACCAGACTCGACATCGCGGGTAAGACGGAAAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                   <  SRR19973069.3562554/150‑1 (MQ=60)
 CCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGG                                                                                                                                                  <  SRR19973069.1638054/150‑1 (MQ=60)
   TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTG                                                                                                                                                <  SRR19973069.2873577/150‑1 (MQ=60)
     CTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTA                                                                                                                                              >  SRR19973069.819241/1‑150 (MQ=60)
       GACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACA                                                                                                                                            <  SRR19973069.1075420/150‑1 (MQ=60)
          CAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCT                                                                                                                                         <  SRR19973069.3568917/150‑1 (MQ=60)
             ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      >  SRR19973069.676817/1‑150 (MQ=60)
             ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      >  SRR19973069.2620732/1‑150 (MQ=60)
             ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      >  SRR19973069.2601527/1‑150 (MQ=60)
             ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      >  SRR19973069.1443310/1‑150 (MQ=60)
             ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      <  SRR19973069.1916269/150‑1 (MQ=60)
               TCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAG                                                                                                                                    <  SRR19973069.3202314/150‑1 (MQ=60)
               TCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAG                                                                                                                                    <  SRR19973069.445371/150‑1 (MQ=60)
                 GACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAT                                                                                                                                  >  SRR19973069.2305609/1‑150 (MQ=60)
                         GGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCT                                                                                                                          <  SRR19973069.3717955/150‑1 (MQ=60)
                           GTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCT                                                                                                                        >  SRR19973069.2046112/1‑150 (MQ=60)
                           GTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTACGCCTCT                                                                                                                        >  SRR19973069.2046315/1‑150 (MQ=60)
                            TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                       <  SRR19973069.2699059/150‑1 (MQ=60)
                            TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                       <  SRR19973069.769041/150‑1 (MQ=60)
                            TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                       <  SRR19973069.769261/150‑1 (MQ=60)
                            TCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTT                                                                                                                       <  SRR19973069.2166317/150‑1 (MQ=60)
                             CAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTT                                                                                                                      <  SRR19973069.2817561/150‑1 (MQ=60)
                             CAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTT                                                                                                                      <  SRR19973069.1513318/150‑1 (MQ=60)
                             CAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTT                                                                                                                      <  SRR19973069.2817642/150‑1 (MQ=60)
                             CAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTT                                                                                                                      <  SRR19973069.2059369/150‑1 (MQ=60)
                                  GGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGG                                                                                                                 >  SRR19973069.2481003/1‑150 (MQ=60)
                                  GGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTACAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGG                                                                                                                 >  SRR19973069.777859/1‑150 (MQ=60)
                                     CAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCA                                                                                                              >  SRR19973069.2165798/1‑150 (MQ=60)
                                      AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                             >  SRR19973069.595329/1‑150 (MQ=60)
                                      AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                             >  SRR19973069.596530/1‑150 (MQ=60)
                                      AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                             >  SRR19973069.3271563/1‑150 (MQ=60)
                                      AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                             >  SRR19973069.595528/1‑150 (MQ=60)
                                      AATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAG                                                                                                             >  SRR19973069.595503/1‑150 (MQ=60)
                                       ATCTCCTGCGGGGTTTTCGGCATCGCCGTTACGCGACCGTTAATTTTGGCTTTTTGGTCGTCCAGCCCCGGACGGTTCTCCAGCCGCGTGCGGTAAGCCGGGTGAACTTAGGTGTACAGCTTTGAGATTTACGCCTAGTTAAAGGTCAGT                                                                                                            >  SRR19973069.2931369/1‑150 (MQ=60)
                                         CTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTC                                                                                                          >  SRR19973069.3838592/1‑150 (MQ=60)
                                         CTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTC                                                                                                          >  SRR19973069.2196718/1‑150 (MQ=60)
                                         CTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTC                                                                                                          >  SRR19973069.2196760/1‑150 (MQ=60)
                                           CCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGT                                                                                                        >  SRR19973069.832662/1‑150 (MQ=60)
                                           CCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGT                                                                                                        >  SRR19973069.832652/1‑150 (MQ=60)
                                             TGCTGTGTTTTCGGAATCGGCGTTTCGCGACCGTTTAGTTTGGGTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTTCGGTTCGCAGGGTGAACTTAGGTGTACAGCTTTGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTC                                                                                                      <  SRR19973069.2842874/150‑1 (MQ=60)
                                                TGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAC                                                                                                   <  SRR19973069.571325/150‑1 (MQ=60)
                                                TGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAC                                                                                                   <  SRR19973069.584779/150‑1 (MQ=60)
                                                TGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTAGTCTTCCAGCGCCGGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCGTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAC                                                                                                   >  SRR19973069.249186/1‑150 (MQ=60)
                                                 GGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACT                                                                                                  <  SRR19973069.1440860/150‑1 (MQ=60)
                                                       TCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCAT                                                                                            <  SRR19973069.78626/150‑1 (MQ=60)
                                                              CGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTT                                                                                     <  SRR19973069.2758134/150‑1 (MQ=60)
                                                               GCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTC                                                                                    >  SRR19973069.1328030/1‑150 (MQ=60)
                                                               GCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTC                                                                                    >  SRR19973069.2511577/1‑150 (MQ=60)
                                                                 CGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCAC                                                                                  <  SRR19973069.2825889/150‑1 (MQ=60)
                                                                   TTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCAACGATGTTCACCA                                                                                >  SRR19973069.1706164/1‑150 (MQ=60)
                                                                        CGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAA                                                                           >  SRR19973069.1689260/1‑150 (MQ=60)
                                                                          ACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATC                                                                         >  SRR19973069.1159186/1‑150 (MQ=60)
                                                                           CCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAATGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAAACG                                                                        >  SRR19973069.2608487/1‑150 (MQ=60)
                                                                             GTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAG                                                                      >  SRR19973069.3704837/1‑150 (MQ=60)
                                                                             GTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAG                                                                      <  SRR19973069.3605351/150‑1 (MQ=60)
                                                                               TAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCA                                                                    <  SRR19973069.391600/150‑1 (MQ=60)
                                                                                    TTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCT                                                               <  SRR19973069.55775/150‑1 (MQ=60)
                                                                                     TGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTT                                                              >  SRR19973069.1885647/1‑150 (MQ=60)
                                                                                      GGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTT                                                             >  SRR19973069.2544835/1‑150 (MQ=60)
                                                                                       GCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTT                                                            >  SRR19973069.1124149/1‑150 (MQ=60)
                                                                                        CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGTGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                           >  SRR19973069.1392756/1‑150 (MQ=60)
                                                                                        CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                           >  SRR19973069.1393264/1‑150 (MQ=60)
                                                                                        CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                           <  SRR19973069.1450824/150‑1 (MQ=60)
                                                                                        CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                           <  SRR19973069.2425699/150‑1 (MQ=60)
                                                                                        CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                           <  SRR19973069.163393/150‑1 (MQ=60)
                                                                                        CTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTG                                                           <  SRR19973069.2425696/150‑1 (MQ=60)
                                                                                              CGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCA                                                     <  SRR19973069.1276979/150‑1 (MQ=60)
                                                                                              CGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCA                                                     <  SRR19973069.796364/150‑1 (MQ=60)
                                                                                              CGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCA                                                     <  SRR19973069.2811062/150‑1 (MQ=60)
                                                                                              CGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTGCACCATCGAATCGAGCACCGCTTTTGCTTCCA                                                     <  SRR19973069.1969817/150‑1 (MQ=60)
                                                                                                 CTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCG                                                  >  SRR19973069.1701578/1‑150 (MQ=60)
                                                                                                     CAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAG                                              >  SRR19973069.2467017/1‑150 (MQ=60)
                                                                                                     CAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAG                                              <  SRR19973069.542813/150‑1 (MQ=60)
                                                                                                     CAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAG                                              <  SRR19973069.475263/150‑1 (MQ=60)
                                                                                                      AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                             >  SRR19973069.1880652/1‑150 (MQ=60)
                                                                                                        CGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATC                                           >  SRR19973069.1040251/1‑150 (MQ=60)
                                                                                                        CGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATC                                           >  SRR19973069.1039191/1‑150 (MQ=60)
                                                                                                         GCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCA                                          <  SRR19973069.1040665/150‑1 (MQ=60)
                                                                                                           CTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAC                                        >  SRR19973069.1713563/1‑150 (MQ=60)
                                                                                                           CTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAC                                        >  SRR19973069.3361239/1‑150 (MQ=60)
                                                                                                            TGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACA                                       <  SRR19973069.601119/150‑1 (MQ=60)
                                                                                                            TGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACA                                       <  SRR19973069.2532404/150‑1 (MQ=60)
                                                                                                                GTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAAT                                   >  SRR19973069.221773/1‑150 (MQ=60)
                                                                                                                  TTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCT                                 <  SRR19973069.311708/150‑1 (MQ=60)
                                                                                                                  TTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCT                                 <  SRR19973069.683342/150‑1 (MQ=60)
                                                                                                                   TCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTT                                <  SRR19973069.2140413/150‑1 (MQ=60)
                                                                                                                      CCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAT                             <  SRR19973069.3543514/150‑1 (MQ=60)
                                                                                                                      CCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAT                             <  SRR19973069.3132270/150‑1 (MQ=60)
                                                                                                                      CCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAT                             <  SRR19973069.3834078/150‑1 (MQ=60)
                                                                                                                      CCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAT                             <  SRR19973069.1992782/150‑1 (MQ=60)
                                                                                                                          CAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTG                         <  SRR19973069.1392980/150‑1 (MQ=60)
                                                                                                                             CGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACG                      <  SRR19973069.2286556/150‑1 (MQ=60)
                                                                                                                             CGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACG                      <  SRR19973069.2285709/150‑1 (MQ=60)
                                                                                                                             CGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACG                      <  SRR19973069.721359/150‑1 (MQ=60)
                                                                                                                                  GGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGG                 >  SRR19973069.3605383/1‑150 (MQ=60)
                                                                                                                                  GGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGG                 <  SRR19973069.2622872/150‑1 (MQ=60)
                                                                                                                                  GGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGG                 <  SRR19973069.2779890/150‑1 (MQ=60)
                                                                                                                                      CGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATT             <  SRR19973069.3453675/150‑1 (MQ=60)
                                                                                                                                                 CTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTT  >  SRR19973069.1512368/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTT  >  CP009273/2127980‑2128274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑