Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,128,128 T→A 100% K336* (AAA→TAA)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,128,1280TA99.5% 572.0 / ‑5.9 172K336* (AAA→TAA) wzccolanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (75/96);  total (75/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT  >  CP009273/2127983‑2128272
                                                                                                                                                 |                                                                                                                                                
 gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGgt                                                                                                                                               <  2:2369917/148‑1 (MQ=255)
 gctgACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGgt                                                                                                                                               >  2:666523/1‑148 (MQ=255)
    gACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTa                                                                                                                                            <  1:1639143/148‑1 (MQ=255)
     aCCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTAc                                                                                                                                           <  1:3226640/148‑1 (MQ=255)
      ccAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACa                                                                                                                                          >  2:431234/1‑148 (MQ=255)
      ccAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTAAGCCGGGTGACCTTAGGTGTACa                                                                                                                                          >  2:2840163/1‑148 (MQ=255)
        aGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGc                                                                                                                                        <  1:1653014/148‑1 (MQ=255)
         gACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCt                                                                                                                                       >  2:180557/1‑148 (MQ=255)
         gACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCt                                                                                                                                       <  1:508264/148‑1 (MQ=255)
          aCTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTATGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCtt                                                                                                                                      >  2:2431201/1‑148 (MQ=255)
          aCTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCtt                                                                                                                                      <  1:3433789/148‑1 (MQ=255)
            tCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTgg                                                                                                                                    <  2:3025841/148‑1 (MQ=255)
               aCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGgaga                                                                                                                                 >  2:898865/1‑148 (MQ=255)
               aCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGgaga                                                                                                                                 <  1:995101/148‑1 (MQ=255)
               aCATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGgaga                                                                                                                                 <  1:1740481/148‑1 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                >  2:1264484/1‑148 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                >  2:3228639/1‑148 (MQ=255)
                cATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAt                                                                                                                                >  2:1044454/1‑148 (MQ=255)
                  tCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAttt                                                                                                                              <  1:3363918/148‑1 (MQ=255)
                          cAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctct                                                                                                                      <  1:798948/148‑1 (MQ=255)
                          cAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCctct                                                                                                                      >  2:1294330/1‑148 (MQ=255)
                           aGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCtt                                                                                                                     >  2:779586/1‑148 (MQ=255)
                             aCGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTa                                                                                                                   >  1:3058514/1‑148 (MQ=255)
                               ggACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTaaa                                                                                                                 <  2:1147432/148‑1 (MQ=255)
                                 aCAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAgg                                                                                                               >  2:1045753/1‑148 (MQ=255)
                                    aTCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCa                                                                                                            <  1:2844676/148‑1 (MQ=255)
                                     tctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAg                                                                                                           <  1:1345499/148‑1 (MQ=255)
                                      ctcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgt                                                                                                          >  2:1701803/1‑148 (MQ=255)
                                       tcCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgtt                                                                                                         >  2:1531313/1‑148 (MQ=255)
                                        ccTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGTTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttc                                                                                                        <  2:73322/148‑1 (MQ=255)
                                         ctgctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcg                                                                                                       <  2:3161004/148‑1 (MQ=255)
                                         ctgctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcg                                                                                                       >  2:2133949/1‑148 (MQ=255)
                                           gctgGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgtt                                                                                                     <  2:520627/148‑1 (MQ=255)
                                              gggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCaa                                                                                                  >  2:3081659/1‑148 (MQ=255)
                                              gggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCaa                                                                                                  <  1:1501259/148‑1 (MQ=255)
                                               ggTTTTCGGCATCGCCGTTACGCGACCGTTAATTTTGGCTTTTTAGTCTTCCAGCGCCAGTCGTTTCTCCAGAACCGTGCGGTAAGCCGGGTGAAAGTAGGTGTACAGCTAGGAGAATTACGCCTCTTTAAAGTTCAGTTAGTTCAAc                                                                                                 >  1:649270/1‑148 (MQ=255)
                                               ggTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAAc                                                                                                 >  2:3118948/1‑148 (MQ=255)
                                                  tttCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTgc                                                                                              <  2:3182904/148‑1 (MQ=255)
                                                         aTCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGa                                                                                       <  1:3081659/148‑1 (MQ=255)
                                                          tCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGAt                                                                                      <  2:2957572/148‑1 (MQ=255)
                                                            gCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGt                                                                                    >  1:2844518/1‑148 (MQ=255)
                                                             ccGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGtt                                                                                   <  1:3488968/148‑1 (MQ=255)
                                                              cGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTc                                                                                  <  2:177716/148‑1 (MQ=255)
                                                                ttACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCAc                                                                                <  1:360549/148‑1 (MQ=255)
                                                                ttACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCAc                                                                                >  1:1996527/1‑148 (MQ=255)
                                                                 tACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCAcc                                                                               >  2:1940307/1‑148 (MQ=255)
                                                                   cgcgACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCat                                                                             <  1:795571/148‑1 (MQ=255)
                                                                     cgACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcg                                                                           >  1:1605884/1‑148 (MQ=255)
                                                                       aCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaa                                                                         <  1:1916424/148‑1 (MQ=255)
                                                                        ccGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaat                                                                        <  2:2497483/148‑1 (MQ=255)
                                                                        ccGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaat                                                                        >  2:3282724/1‑148 (MQ=255)
                                                                        ccGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaat                                                                        <  1:588150/148‑1 (MQ=255)
                                                                         cGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatc                                                                       <  1:1159264/148‑1 (MQ=255)
                                                                           ttAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatcga                                                                     >  1:1145826/1‑148 (MQ=255)
                                                                            tAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAg                                                                    >  2:1592461/1‑148 (MQ=255)
                                                                              aGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCa                                                                  <  2:2017741/148‑1 (MQ=255)
                                                                                tttGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAcc                                                                >  2:2035154/1‑148 (MQ=255)
                                                                                tttGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCAACGATGTTCACCATCGAATAGAGCAcc                                                                >  1:2622486/1‑148 (MQ=255)
                                                                                tttGGCTTTTTCGTCTTCCAGCGCCTGACGTTTATCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTTTACAGCTTGGAGATTTACGCCTCTTTAAATGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCaac                                                                >  1:1974801/1‑146 (MQ=255)
                                                                                 ttGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCg                                                               <  1:2619666/148‑1 (MQ=255)
                                                                                    gCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtt                                                            <  1:1233042/148‑1 (MQ=255)
                                                                                      tttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtttt                                                          >  1:2566101/1‑148 (MQ=255)
                                                                                          tCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCtt                                                      >  1:2054654/1‑148 (MQ=255)
                                                                                          tCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCtt                                                      >  1:3204990/1‑148 (MQ=255)
                                                                                            gTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTcc                                                    <  1:304341/148‑1 (MQ=255)
                                                                                             tCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCa                                                   <  2:2864849/148‑1 (MQ=255)
                                                                                              cTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAg                                                  >  2:66544/1‑148 (MQ=255)
                                                                                               ttCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGc                                                 >  2:147353/1‑148 (MQ=255)
                                                                                                tCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCg                                                >  2:1396362/1‑148 (MQ=255)
                                                                                                 ccAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCgg                                               <  2:3363028/148‑1 (MQ=255)
                                                                                                  cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGc                                              <  1:1044454/148‑1 (MQ=255)
                                                                                                  cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGc                                              <  1:2197828/148‑1 (MQ=255)
                                                                                                  cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGc                                              <  1:3323422/148‑1 (MQ=255)
                                                                                                    gcgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAg                                            <  1:3169235/148‑1 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  1:89548/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           <  2:2541749/148‑1 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  1:89533/1‑148 (MQ=255)
                                                                                                     cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGa                                           >  1:89522/1‑148 (MQ=255)
                                                                                                      gcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGAt                                          <  1:1044056/148‑1 (MQ=255)
                                                                                                      gcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGAt                                          <  1:2656878/148‑1 (MQ=255)
                                                                                                             gTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGa                                   <  1:2484629/148‑1 (MQ=255)
                                                                                                              tttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGaa                                  >  2:535764/1‑148 (MQ=255)
                                                                                                               ttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAAt                                 <  2:3113923/148‑1 (MQ=255)
                                                                                                                   ccAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCttt                             <  1:180557/148‑1 (MQ=255)
                                                                                                                      gcagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATc                          >  2:33650/1‑148 (MQ=255)
                                                                                                                      gcagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGGTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATc                          <  1:2133949/148‑1 (MQ=255)
                                                                                                                      gcagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCATCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATc                          <  2:196863/148‑1 (MQ=255)
                                                                                                                       cagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATcc                         >  1:374659/1‑148 (MQ=255)
                                                                                                                       cagcGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATcc                         <  1:1294330/148‑1 (MQ=255)
                                                                                                                            tGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACg                    >  1:2415859/1‑148 (MQ=255)
                                                                                                                              cGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGa                  <  2:645536/148‑1 (MQ=255)
                                                                                                                              cGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGa                  >  1:1943675/1‑148 (MQ=255)
                                                                                                                                 tACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAgg               <  1:1469167/148‑1 (MQ=255)
                                                                                                                                    gCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCAt            <  2:2627172/148‑1 (MQ=255)
                                                                                                                                      cGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTc          <  1:3418490/148‑1 (MQ=255)
                                                                                                                                         gTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGGTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  2:2140453/148‑1 (MQ=255)
                                                                                                                                         gTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       <  1:765524/148‑1 (MQ=255)
                                                                                                                                         gTGAACTAAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgt       >  1:2367433/1‑148 (MQ=255)
                                                                                                                                             aCTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgt   <  2:1199591/148‑1 (MQ=255)
                                                                                                                                             aCTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgt   <  2:2067414/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTT  >  CP009273/2127983‑2128272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTT  >  CP009273/2127979‑2128274
                                                                                                                                                     |                                                                                                                                                  
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTA                                                                                                                                                    >  SRR19973065.973527/1‑150 (MQ=60)
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTA                                                                                                                                                    >  SRR19973065.973215/1‑150 (MQ=60)
 ACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAG                                                                                                                                                   <  SRR19973065.5759/150‑1 (MQ=60)
    TGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTG                                                                                                                                                <  SRR19973065.1522235/150‑1 (MQ=60)
        GACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACA                                                                                                                                            <  SRR19973065.1650153/150‑1 (MQ=60)
         ACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAG                                                                                                                                           <  SRR19973065.3246012/150‑1 (MQ=60)
            AGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTT                                                                                                                                        <  SRR19973065.1664099/150‑1 (MQ=60)
             GACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTG                                                                                                                                       <  SRR19973065.511644/150‑1 (MQ=60)
              ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      <  SRR19973065.3453681/150‑1 (MQ=60)
              ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      <  SRR19973065.374788/150‑1 (MQ=60)
              ACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGG                                                                                                                                      <  SRR19973065.3454741/150‑1 (MQ=60)
                  GACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAT                                                                                                                                  <  SRR19973065.3091296/150‑1 (MQ=60)
                  GACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAT                                                                                                                                  <  SRR19973065.2508082/150‑1 (MQ=60)
                   ACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATT                                                                                                                                 <  SRR19973065.1751973/150‑1 (MQ=60)
                   ACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATT                                                                                                                                 <  SRR19973065.1001716/150‑1 (MQ=60)
                      TCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCC                                                                                                                              <  SRR19973065.3384534/150‑1 (MQ=60)
                      TCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCC                                                                                                                              <  SRR19973065.3384485/150‑1 (MQ=60)
                            GTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCT                                                                                                                        >  SRR19973065.1732877/1‑150 (MQ=60)
                              CAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTT                                                                                                                      <  SRR19973065.804228/150‑1 (MQ=60)
                               AGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTA                                                                                                                     >  SRR19973065.2159389/1‑150 (MQ=60)
                               AGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTA                                                                                                                     >  SRR19973065.3077208/1‑150 (MQ=60)
                               AGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTA                                                                                                                     >  SRR19973065.2633129/1‑150 (MQ=60)
                               AGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGAGTTCCGCCTCTTTA                                                                                                                     >  SRR19973065.3076784/1‑150 (MQ=60)
                                  CGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAG                                                                                                                  <  SRR19973065.1067567/150‑1 (MQ=60)
                                        ATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGT                                                                                                            <  SRR19973065.2862183/150‑1 (MQ=60)
                                         TCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTT                                                                                                           <  SRR19973065.1354396/150‑1 (MQ=60)
                                          CTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTC                                                                                                          >  SRR19973065.2643423/1‑150 (MQ=60)
                                                 TGGGTTTTCGGCATCGCCGTTACGCGACCGTTAATTTTGGCTTTTTAGTCTTCCAGCGCCAGTCGTTTCTCCAGAACCGTGCGGTAAGCCGGGTGAAAGTAGGTGTACAGCTAGGAGAATTACGCCTCTTTAAAGTTCAGTTAGTTCAAC                                                                                                   >  SRR19973065.653543/1‑150 (MQ=60)
                                                  GGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACT                                                                                                  <  SRR19973065.1510956/150‑1 (MQ=60)
                                                             ATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATG                                                                                       <  SRR19973065.3100478/150‑1 (MQ=60)
                                                              TCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGT                                                                                      >  SRR19973065.2862023/1‑150 (MQ=60)
                                                              TCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGT                                                                                      >  SRR19973065.2862748/1‑150 (MQ=60)
                                                               CGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTT                                                                                     <  SRR19973065.1969558/150‑1 (MQ=60)
                                                               CGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTT                                                                                     <  SRR19973065.1265977/150‑1 (MQ=60)
                                                                 CCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCA                                                                                   <  SRR19973065.3510186/150‑1 (MQ=60)
                                                                  CGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCAC                                                                                  >  SRR19973065.2009460/1‑150 (MQ=60)
                                                                    TTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCA                                                                                <  SRR19973065.363081/150‑1 (MQ=60)
                                                                       CGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCG                                                                             <  SRR19973065.800833/150‑1 (MQ=60)
                                                                       CGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCG                                                                             <  SRR19973065.800936/150‑1 (MQ=60)
                                                                       CGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCG                                                                             >  SRR19973065.1618401/1‑150 (MQ=60)
                                                                       CGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCG                                                                             >  SRR19973065.1616631/1‑150 (MQ=60)
                                                                       CGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCG                                                                             <  SRR19973065.3224869/150‑1 (MQ=60)
                                                                         CGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCGTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCAACGAA                                                                           >  SRR19973065.623508/1‑150 (MQ=60)
                                                                          GACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAAT                                                                          <  SRR19973065.1756182/150‑1 (MQ=60)
                                                                           ACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATC                                                                         <  SRR19973065.1928997/150‑1 (MQ=60)
                                                                           ACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATC                                                                         <  SRR19973065.589342/150‑1 (MQ=60)
                                                                            CCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCG                                                                        <  SRR19973065.3527193/150‑1 (MQ=60)
                                                                            CCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCG                                                                        <  SRR19973065.591802/150‑1 (MQ=60)
                                                                             CGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGA                                                                       <  SRR19973065.1166692/150‑1 (MQ=60)
                                                                             CGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGA                                                                       >  SRR19973065.1153392/1‑150 (MQ=60)
                                                                             CGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGA                                                                       >  SRR19973065.1153220/1‑150 (MQ=60)
                                                                              GATAAGTTTGGCTTTTTAGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCAACGATGTTAACCAACGAATCGAG                                                                      >  SRR19973065.322626/1‑150 (MQ=60)
                                                                                  AGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCAACGATGTTCACCATCGAATAGAGCACC                                                                  >  SRR19973065.2638718/1‑150 (MQ=60)
                                                                                  AGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTATCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTTTACAGCTTGGAGATTTACGCCTCTTTAAATGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAAC                                                                  >  SRR19973065.1987658/1‑150 (MQ=60)
                                                                                     TTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCT                                                               <  SRR19973065.2635883/150‑1 (MQ=60)
                                                                                        GCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTT                                                            >  SRR19973065.2582128/1‑150 (MQ=60)
                                                                                        GCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTT                                                            <  SRR19973065.1241117/150‑1 (MQ=60)
                                                                                           TTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCT                                                         >  SRR19973065.1375548/1‑150 (MQ=60)
                                                                                            TTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTT                                                        >  SRR19973065.2067774/1‑150 (MQ=60)
                                                                                            TTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTT                                                        >  SRR19973065.3224283/1‑150 (MQ=60)
                                                                                                GTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAG                                                    <  SRR19973065.306358/150‑1 (MQ=60)
                                                                                                      CAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAG                                              <  SRR19973065.1051357/150‑1 (MQ=60)
                                                                                                      CAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAG                                              <  SRR19973065.2211904/150‑1 (MQ=60)
                                                                                                      CAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAG                                              <  SRR19973065.3343701/150‑1 (MQ=60)
                                                                                                       AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                             >  SRR19973065.90380/1‑150 (MQ=60)
                                                                                                       AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                             >  SRR19973065.90405/1‑150 (MQ=60)
                                                                                                       AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                             >  SRR19973065.90390/1‑150 (MQ=60)
                                                                                                       AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                             >  SRR19973065.90379/1‑150 (MQ=60)
                                                                                                       AGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGA                                             >  SRR19973065.90356/1‑150 (MQ=60)
                                                                                                        GCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGAT                                            <  SRR19973065.3188419/150‑1 (MQ=60)
                                                                                                          GCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCA                                          <  SRR19973065.2673298/150‑1 (MQ=60)
                                                                                                          GCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCA                                          <  SRR19973065.1050957/150‑1 (MQ=60)
                                                                                                           CCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAA                                         >  SRR19973065.2096259/1‑150 (MQ=60)
                                                                                                              GACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAG                                      <  SRR19973065.1996347/150‑1 (MQ=60)
                                                                                                                 GTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAAT                                   <  SRR19973065.2500264/150‑1 (MQ=60)
                                                                                                                     CTCCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTT                               <  SRR19973065.335841/150‑1 (MQ=60)
                                                                                                                       CCAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAT                             <  SRR19973065.181985/150‑1 (MQ=60)
                                                                                                                        CAGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATC                            <  SRR19973065.2446539/150‑1 (MQ=60)
                                                                                                                         AGCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCC                           >  SRR19973065.377277/1‑150 (MQ=60)
                                                                                                                          GCAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGGTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCT                          <  SRR19973065.2147685/150‑1 (MQ=60)
                                                                                                                           CAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTG                         <  SRR19973065.1302858/150‑1 (MQ=60)
                                                                                                                           CAGCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTG                         <  SRR19973065.1303741/150‑1 (MQ=60)
                                                                                                                             GCGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGAC                       <  SRR19973065.670959/150‑1 (MQ=60)
                                                                                                                              CGTGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACG                      >  SRR19973065.2431076/1‑150 (MQ=60)
                                                                                                                                TGCGGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGA                    >  SRR19973065.1956379/1‑150 (MQ=60)
                                                                                                                                   GGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGG                 <  SRR19973065.1828278/150‑1 (MQ=60)
                                                                                                                                   GGTACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGG                 <  SRR19973065.2357785/150‑1 (MQ=60)
                                                                                                                                     TACGCCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCA               <  SRR19973065.1478589/150‑1 (MQ=60)
                                                                                                                                         CCGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCA           <  SRR19973065.2857650/150‑1 (MQ=60)
                                                                                                                                          CGGGTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAG          <  SRR19973065.3439375/150‑1 (MQ=60)
                                                                                                                                           GGGTGAACTAAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGT         >  SRR19973065.2382184/1‑150 (MQ=60)
                                                                                                                                             GTGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTT       <  SRR19973065.770613/150‑1 (MQ=60)
                                                                                                                                              TGAACTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTG      <  SRR19973065.904621/150‑1 (MQ=60)
                                                                                                                                                  CTTAGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTT  >  SRR19973065.615428/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTT  >  CP009273/2127979‑2128274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑