Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,216,296 C→A 100% I211I (ATC→ATA)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,216,2960CA99.5% 705.3 / ‑4.2 217I211I (ATC→ATA) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (105/111);  total (106/111)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

TGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAG  >  CP009273/2216151‑2216440
                                                                                                                                                 |                                                                                                                                                
tGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATaaa                                                                                                                                                <  2:844549/148‑1 (MQ=255)
tGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATaaa                                                                                                                                                <  1:1283758/148‑1 (MQ=255)
  gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAg                                                                                                                                              <  2:1117251/148‑1 (MQ=255)
  gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAg                                                                                                                                              <  1:2389406/148‑1 (MQ=255)
  gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAg                                                                                                                                              <  1:3239936/148‑1 (MQ=255)
   tGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGa                                                                                                                                             <  1:858198/148‑1 (MQ=255)
     cAACGTGGCCCGGCGTATACCGACATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                           <  2:1317472/148‑1 (MQ=255)
     cAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                           >  2:2700975/1‑148 (MQ=255)
     cAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                           <  2:3531810/148‑1 (MQ=255)
        cGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACg                                                                                                                                        >  2:1770185/1‑148 (MQ=255)
             cccGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAtgtg                                                                                                                                   <  2:1943945/148‑1 (MQ=255)
              ccGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGc                                                                                                                                  >  1:983937/1‑148 (MQ=255)
                    tataCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACg                                                                                                                            >  2:1135992/1‑148 (MQ=255)
                    tataCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACg                                                                                                                            >  1:1108295/1‑148 (MQ=255)
                      taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAAATTACGCTGGTGAACCATCTGGGGATTTATCTGGGCGAAAAGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGAt                                                                                                                          >  1:2959878/1‑148 (MQ=255)
                       aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATg                                                                                                                         >  2:582781/1‑148 (MQ=255)
                        ccGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATgg                                                                                                                        >  1:2089855/1‑148 (MQ=255)
                         cGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgt                                                                                                                       >  2:1276201/1‑148 (MQ=255)
                           aaaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcg                                                                                                                     >  1:1059252/1‑148 (MQ=255)
                            aaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgt                                                                                                                    >  2:2556021/1‑148 (MQ=255)
                             aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                   >  1:3145392/1‑148 (MQ=255)
                             aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                   <  1:2579622/148‑1 (MQ=255)
                                  gTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGcc                                                                                                              <  2:2037613/148‑1 (MQ=255)
                                  gTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGcc                                                                                                              <  2:2967959/148‑1 (MQ=255)
                                    tATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCa                                                                                                            >  2:416210/1‑148 (MQ=255)
                                       tCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGa                                                                                                         <  1:1472512/148‑1 (MQ=255)
                                        cgcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAt                                                                                                        <  2:2113254/148‑1 (MQ=255)
                                        cgcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAt                                                                                                        <  1:3016578/148‑1 (MQ=255)
                                            cgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATg                                                                                                    >  1:3294525/1‑148 (MQ=255)
                                            cgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATg                                                                                                    >  2:1420123/1‑148 (MQ=255)
                                            cgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATg                                                                                                    <  2:1800736/148‑1 (MQ=255)
                                            cgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATg                                                                                                    <  1:1819025/148‑1 (MQ=255)
                                             gTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGa                                                                                                   >  1:680027/1‑148 (MQ=255)
                                              tataAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGAc                                                                                                  >  2:2204/1‑148 (MQ=255)
                                                taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTa                                                                                                <  1:2878341/148‑1 (MQ=255)
                                                taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTa                                                                                                >  1:554097/1‑148 (MQ=255)
                                                 aaaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAt                                                                                               >  2:3422836/1‑148 (MQ=255)
                                                  aaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATg                                                                                              <  1:24756/148‑1 (MQ=255)
                                                   aTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGt                                                                                             >  1:2177810/1‑148 (MQ=255)
                                                   aTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGt                                                                                             <  2:883368/148‑1 (MQ=255)
                                                            gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGt                                                                                    <  1:1454688/148‑1 (MQ=255)
                                                            gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGt                                                                                    <  1:806590/148‑1 (MQ=255)
                                                            gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGt                                                                                    <  1:806742/148‑1 (MQ=255)
                                                                aCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGt                                                                                >  2:1648858/1‑148 (MQ=255)
                                                                    aCGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGata                                                                            <  1:2787535/148‑1 (MQ=255)
                                                                    aCGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGata                                                                            >  2:2829338/1‑148 (MQ=255)
                                                                     cGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGatat                                                                           >  2:2143587/1‑148 (MQ=255)
                                                                       cTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTg                                                                         <  2:1209622/148‑1 (MQ=255)
                                                                         ggTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGaa                                                                       >  1:1088703/1‑148 (MQ=255)
                                                                           tGACCCATCTGGGGATTGATCGGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGTATGATGCTCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCTCGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGc                                                                     <  1:2369173/148‑1 (MQ=255)
                                                                             aaCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATCATGACTATGTCCACCGCGTTCGTGATATTGAAGCCg                                                                   <  2:497156/148‑1 (MQ=255)
                                                                               ccATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGac                                                                 >  1:873051/1‑148 (MQ=255)
                                                                                cATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGaca                                                                <  1:2675384/148‑1 (MQ=255)
                                                                                   cTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGc                                                             >  1:3566025/1‑148 (MQ=255)
                                                                                    tGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGcc                                                            <  2:483681/148‑1 (MQ=255)
                                                                                     ggggATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGccc                                                           >  2:2153190/1‑148 (MQ=255)
                                                                                         aTTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCAc                                                       >  1:2433071/1‑148 (MQ=255)
                                                                                           tGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGt                                                     >  1:1545107/1‑148 (MQ=255)
                                                                                             aTCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTta                                                   >  1:3172313/1‑148 (MQ=255)
                                                                                              tCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTtat                                                  >  1:2474601/1‑148 (MQ=255)
                                                                                               cTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTtata                                                 <  2:1286868/148‑1 (MQ=255)
                                                                                                 gggCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAAc                                               >  2:2366562/1‑148 (MQ=255)
                                                                                                  ggCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACg                                              <  1:2355121/148‑1 (MQ=255)
                                                                                                   gCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGc                                             <  1:1155268/148‑1 (MQ=255)
                                                                                                     gAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCg                                           <  2:2539342/148‑1 (MQ=255)
                                                                                                       aaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGAt                                         <  1:3020830/148‑1 (MQ=255)
                                                                                                        aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                        <  2:3489943/148‑1 (MQ=255)
                                                                                                        aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                        >  1:2527271/1‑148 (MQ=255)
                                                                                                        aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                        <  2:543674/148‑1 (MQ=255)
                                                                                                         cGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                       <  2:1088703/148‑1 (MQ=255)
                                                                                                            cGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                    >  2:3042214/1‑148 (MQ=255)
                                                                                                            cGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                    >  1:465015/1‑148 (MQ=255)
                                                                                                               aGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCg                                 >  1:1410059/1‑148 (MQ=255)
                                                                                                               aGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCg                                 >  2:228666/1‑148 (MQ=255)
                                                                                                                gCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCgg                                <  2:2177810/148‑1 (MQ=255)
                                                                                                                    aTCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                            >  1:191272/1‑148 (MQ=255)
                                                                                                                    aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                            >  2:3390145/1‑148 (MQ=255)
                                                                                                                    aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                            <  1:2153190/148‑1 (MQ=255)
                                                                                                                     tCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                           <  1:1770185/148‑1 (MQ=255)
                                                                                                                      ccTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAttt                          >  2:2210945/1‑148 (MQ=255)
                                                                                                                      ccTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAttt                          <  1:3371252/148‑1 (MQ=255)
                                                                                                                       cTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                         <  2:452923/148‑1 (MQ=255)
                                                                                                                        ttAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGa                        <  2:1245685/148‑1 (MQ=255)
                                                                                                                          aGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAt                      <  1:2829083/148‑1 (MQ=255)
                                                                                                                          aGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAt                      <  1:1609143/148‑1 (MQ=255)
                                                                                                                           gCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtc                     >  2:1480949/1‑148 (MQ=255)
                                                                                                                           gCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtc                     <  2:3303043/148‑1 (MQ=255)
                                                                                                                           gCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtc                     <  2:422817/148‑1 (MQ=255)
                                                                                                                            cAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtct                    >  2:1512226/1‑148 (MQ=255)
                                                                                                                            cAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtct                    <  1:166192/148‑1 (MQ=255)
                                                                                                                             aaGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtctt                   <  1:3426292/148‑1 (MQ=255)
                                                                                                                               gCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtcttct                 >  2:2348542/1‑148 (MQ=255)
                                                                                                                                  tgatgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTTGt              <  1:2969424/147‑1 (MQ=255)
                                                                                                                                  ggATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGt              >  2:2849301/1‑148 (MQ=255)
                                                                                                                                   gatgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGtt             >  1:401429/1‑148 (MQ=255)
                                                                                                                                    atgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTg            <  2:873008/148‑1 (MQ=255)
                                                                                                                                       atgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc         >  1:1753660/1‑148 (MQ=255)
                                                                                                                                          atCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCgg      >  1:3081100/1‑148 (MQ=255)
                                                                                                                                              cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAg  <  1:1242972/148‑1 (MQ=255)
                                                                                                                                              cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAg  <  2:1978535/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAG  >  CP009273/2216151‑2216440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  >  CP009273/2216150‑2216442
                                                                                                                                                  |                                                                                                                                                  
CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAA                                                                                                                                                 <  SRR19973066.1272258/150‑1 (MQ=60)
 TGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAG                                                                                                                                                >  SRR19973066.140486/1‑150 (MQ=60)
 TGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAG                                                                                                                                                <  SRR19973066.1293028/150‑1 (MQ=60)
   GTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAT                                                                                                                                              <  SRR19973066.3261314/150‑1 (MQ=60)
   GTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAT                                                                                                                                              <  SRR19973066.2405493/150‑1 (MQ=60)
    TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                             <  SRR19973066.789986/150‑1 (MQ=60)
    TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                             <  SRR19973066.1558248/150‑1 (MQ=60)
    TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                             <  SRR19973066.864382/150‑1 (MQ=60)
      CAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAC                                                                                                                                           >  SRR19973066.908182/1‑150 (MQ=60)
             GCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGC                                                                                                                                    >  SRR19973066.991198/1‑150 (MQ=60)
                   CGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACG                                                                                                                              >  SRR19973066.2373022/1‑150 (MQ=60)
                   CGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACG                                                                                                                              >  SRR19973066.1116317/1‑150 (MQ=60)
                   CGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACG                                                                                                                              >  SRR19973066.1116282/1‑150 (MQ=60)
                     TATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAAATTACGCTGGTGAACCATCTGGGGATTTATCTGGGCGAAAAGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGAT                                                                                                                            >  SRR19973066.2979485/1‑150 (MQ=60)
                       TACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGG                                                                                                                          >  SRR19973066.2104256/1‑150 (MQ=60)
                        ACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGT                                                                                                                         >  SRR19973066.3294511/1‑150 (MQ=60)
                         CCTAAATGTCGTTAGTCGCGCGTATAAATGAAGACGGCAAACTGCCGCTGGTGAAACATCTGGTGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                        <  SRR19973066.2686811/150‑1 (MQ=60)
                          CGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCG                                                                                                                       >  SRR19973066.1067031/1‑150 (MQ=60)
                            AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                     >  SRR19973066.3164510/1‑150 (MQ=60)
                            AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                     >  SRR19973066.3166372/1‑150 (MQ=60)
                            AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                     >  SRR19973066.3166552/1‑150 (MQ=60)
                              ATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCAC                                                                                                                   <  SRR19973066.2597115/150‑1 (MQ=60)
                                  CGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCC                                                                                                               >  SRR19973066.166842/1‑150 (MQ=60)
                                  CGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCC                                                                                                               >  SRR19973066.3436410/1‑150 (MQ=60)
                                  CGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCC                                                                                                               <  SRR19973066.1924895/150‑1 (MQ=60)
                                     TATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACG                                                                                                            >  SRR19973066.1135518/1‑150 (MQ=60)
                                        ttcctatctTAAATGAAGACGGCAAACTGACGCTGGTTAACCATCTGGGGATTGATCTGGTAGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATT                                                                                                         <  SRR19973066.3067708/141‑1 (MQ=60)
                                        TCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATT                                                                                                         <  SRR19973066.1482861/150‑1 (MQ=60)
                                        TCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATT                                                                                                         <  SRR19973066.1482892/150‑1 (MQ=60)
                                         CGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTA                                                                                                        >  SRR19973066.1546983/1‑150 (MQ=60)
                                         CGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTA                                                                                                        <  SRR19973066.3036687/150‑1 (MQ=60)
                                         CGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTA                                                                                                        <  SRR19973066.3036727/150‑1 (MQ=60)
                                           CGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATG                                                                                                      >  SRR19973066.3316275/1‑150 (MQ=60)
                                            gatTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGA                                                                                                     <  SRR19973066.818694/147‑1 (MQ=60)
                                            GCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGA                                                                                                     >  SRR19973066.685012/1‑150 (MQ=60)
                                             CGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGAC                                                                                                    <  SRR19973066.1831988/150‑1 (MQ=60)
                                               TATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTA                                                                                                  >  SRR19973066.558073/1‑150 (MQ=60)
                                               TATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTA                                                                                                  <  SRR19973066.124772/150‑1 (MQ=60)
                                                 TAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATG                                                                                                <  SRR19973066.2897627/150‑1 (MQ=60)
                                                  AAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGT                                                                                               >  SRR19973066.2192929/1‑150 (MQ=60)
                                                  AAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGT                                                                                               >  SRR19973066.2192930/1‑150 (MQ=60)
                                                   AATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTC                                                                                              <  SRR19973066.25112/150‑1 (MQ=60)
                                                   AATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTC                                                                                              <  SRR19973066.28171/150‑1 (MQ=60)
                                                     TGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCA                                                                                            <  SRR19973066.3249945/150‑1 (MQ=60)
                                                       AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                          <  SRR19973066.2867804/150‑1 (MQ=60)
                                                       AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                          <  SRR19973066.2867814/150‑1 (MQ=60)
                                                       AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                          >  SRR19973066.1615086/1‑150 (MQ=60)
                                                       AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                          <  SRR19973066.2051612/150‑1 (MQ=60)
                                                        AGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCG                                                                                         >  SRR19973066.1805343/1‑150 (MQ=60)
                                                             GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                    <  SRR19973066.812633/150‑1 (MQ=60)
                                                             GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                    <  SRR19973066.812666/150‑1 (MQ=60)
                                                             GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                    <  SRR19973066.812513/150‑1 (MQ=60)
                                                             GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                    <  SRR19973066.1464717/150‑1 (MQ=60)
                                                             GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                    <  SRR19973066.1465001/150‑1 (MQ=60)
                                                                AACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGAGGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTG                                                                                 >  SRR19973066.2556603/1‑150 (MQ=60)
                                                                 ACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGA                                                                                <  SRR19973066.641779/150‑1 (MQ=60)
                                                                 ACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGA                                                                                <  SRR19973066.2443681/150‑1 (MQ=60)
                                                                  CTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGAT                                                                               >  SRR19973066.2984587/1‑150 (MQ=60)
                                                                     ACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATT                                                                            <  SRR19973066.2806301/150‑1 (MQ=60)
                                                                      CGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTG                                                                           <  SRR19973066.2458072/150‑1 (MQ=60)
                                                                        CTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAA                                                                         >  SRR19973066.1096644/1‑150 (MQ=60)
                                                                            TGACCCATCTGGGGATTGATCGGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGTATGATGCTCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCTCGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCG                                                                     <  SRR19973066.2385199/150‑1 (MQ=60)
                                                                              AACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGAC                                                                   >  SRR19973066.879295/1‑150 (MQ=60)
                                                                              AACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGAC                                                                   >  SRR19973066.879252/1‑150 (MQ=60)
                                                                                 CATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACG                                                                <  SRR19973066.2693253/150‑1 (MQ=60)
                                                                                  ATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGC                                                               >  SRR19973066.3589662/1‑150 (MQ=60)
                                                                                    CTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCC                                                             >  SRR19973066.396315/1‑150 (MQ=60)
                                                                                        GGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCAC                                                         >  SRR19973066.2449634/1‑150 (MQ=60)
                                                                                          ATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGT                                                       >  SRR19973066.1556164/1‑150 (MQ=60)
                                                                                          ATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGT                                                       >  SRR19973066.3336228/1‑150 (MQ=60)
                                                                                            TGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTA                                                     >  SRR19973066.3193436/1‑150 (MQ=60)
                                                                                             GATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTAT                                                    >  SRR19973066.2491478/1‑150 (MQ=60)
                                                                                             GATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTAT                                                    >  SRR19973066.2491490/1‑150 (MQ=60)
                                                                                               TCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAA                                                  <  SRR19973066.3061136/150‑1 (MQ=60)
                                                                                                   GGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCC                                              <  SRR19973066.2371109/150‑1 (MQ=60)
                                                                                                    GCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCG                                             <  SRR19973066.1163483/150‑1 (MQ=60)
                                                                                                     CGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGA                                            >  SRR19973066.869569/1‑150 (MQ=60)
                                                                                                     CGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGA                                            >  SRR19973066.1082454/1‑150 (MQ=60)
                                                                                                       AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                          >  SRR19973066.470657/1‑150 (MQ=60)
                                                                                                       AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                          >  SRR19973066.470663/1‑150 (MQ=60)
                                                                                                       AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                          >  SRR19973066.2544456/1‑150 (MQ=60)
                                                                                                       AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                          >  SRR19973066.2544447/1‑150 (MQ=60)
                                                                                                       AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                          >  SRR19973066.2544379/1‑150 (MQ=60)
                                                                                                       AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                          >  SRR19973066.3076769/1‑150 (MQ=60)
                                                                                                        AACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCC                                         >  SRR19973066.2843359/1‑150 (MQ=60)
                                                                                                        AACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCC                                         <  SRR19973066.3040981/150‑1 (MQ=60)
                                                                                                           GCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGA                                      >  SRR19973066.468563/1‑150 (MQ=60)
                                                                                                             CGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATC                                    >  SRR19973066.3476329/1‑150 (MQ=60)
                                                                                                              GGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCG                                   >  SRR19973066.1420187/1‑150 (MQ=60)
                                                                                                              GGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCG                                   >  SRR19973066.1746837/1‑150 (MQ=60)
                                                                                                                 GCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTT                                <  SRR19973066.1287116/150‑1 (MQ=60)
                                                                                                                 GCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTT                                <  SRR19973066.1285415/150‑1 (MQ=60)
                                                                                                                   AGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAT                              >  SRR19973066.192922/1‑150 (MQ=60)
                                                                                                                    GATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATT                             <  SRR19973066.419460/150‑1 (MQ=60)
                                                                                                                     ATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTT                            <  SRR19973066.2168139/150‑1 (MQ=60)
                                                                                                                      TCCTTAGCAAGCTTGATGATGATCGCATAAAATTTGACGATGTTCGTCACGTTGGTCGTCACGCCCACTATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACAAGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                           <  SRR19973066.2972863/150‑1 (MQ=60)
                                                                                                                      TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                           <  SRR19973066.1782864/150‑1 (MQ=60)
                                                                                                                      TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                           <  SRR19973066.2382584/150‑1 (MQ=60)
                                                                                                                       CCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGA                          <  SRR19973066.3393744/150‑1 (MQ=60)
                                                                                                                       CCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGA                          <  SRR19973066.3393497/150‑1 (MQ=60)
                                                                                                                          TAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATC                       >  SRR19973066.302611/1‑150 (MQ=60)
                                                                                                                           AGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCT                      <  SRR19973066.2848090/150‑1 (MQ=60)
                                                                                                                           AGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCT                      <  SRR19973066.2235/150‑1 (MQ=60)
                                                                                                                           AGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCT                      <  SRR19973066.1620818/150‑1 (MQ=60)
                                                                                                                             CAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTC                    <  SRR19973066.167697/150‑1 (MQ=60)
                                                                                                                              AAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCT                   <  SRR19973066.3449205/150‑1 (MQ=60)
                                                                                                                               AGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTG                  <  SRR19973066.2137108/150‑1 (MQ=60)
                                                                                                                                  TGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTT               >  SRR19973066.404589/1‑150 (MQ=60)
                                                                                                                                   TGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTTGTTG              <  SRR19973066.2989058/150‑1 (MQ=60)
                                                                                                                                      TGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGC           >  SRR19973066.1766268/1‑150 (MQ=60)
                                                                                                                                      TGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGC           >  SRR19973066.1766349/1‑150 (MQ=60)
                                                                                                                                         TGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGG        >  SRR19973066.3101682/1‑150 (MQ=60)
                                                                                                                                            TCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAA     <  SRR19973066.2394600/150‑1 (MQ=60)
                                                                                                                                             CGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAG    >  SRR19973066.1901389/1‑150 (MQ=60)
                                                                                                                                               CATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  <  SRR19973066.1251872/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                  
CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  >  CP009273/2216150‑2216442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑