Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,216,296 C→A 100% I211I (ATC→ATA)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,216,2960CA99.6% 764.8 / ‑4.4 230I211I (ATC→ATA) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (114/115);  total (114/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGA  >  CP009273/2216150‑2216438
                                                                                                                                                  |                                                                                                                                              
cTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATaaa                                                                                                                                              <  1:426830/149‑1 (MQ=255)
  ggTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAg                                                                                                                                            <  1:1378842/149‑1 (MQ=255)
   gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGa                                                                                                                                           >  1:226386/1‑149 (MQ=255)
     gCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                         >  1:928230/1‑149 (MQ=255)
     gCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                         >  1:2777679/1‑149 (MQ=255)
     gCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                         >  1:2777665/1‑149 (MQ=255)
       aaCGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAc                                                                                                                                       <  1:547117/149‑1 (MQ=255)
       aaCGGGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAc                                                                                                                                       >  2:3158619/1‑149 (MQ=255)
         cGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGa                                                                                                                                     <  1:759518/149‑1 (MQ=255)
          gTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAt                                                                                                                                    <  1:1016112/149‑1 (MQ=255)
            ggCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAtgt                                                                                                                                  <  2:1381245/149‑1 (MQ=255)
             gCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAtgtg                                                                                                                                 >  2:1972861/1‑149 (MQ=255)
              cccGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGc                                                                                                                                <  1:1938954/149‑1 (MQ=255)
               ccGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCg                                                                                                                               <  2:281747/149‑1 (MQ=255)
                 ggCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTc                                                                                                                             >  1:1769415/1‑149 (MQ=255)
                      ataCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGAt                                                                                                                        <  2:1523699/149‑1 (MQ=255)
                       taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATg                                                                                                                       <  2:3086939/149‑1 (MQ=255)
                        aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATgg                                                                                                                      <  2:3872107/149‑1 (MQ=255)
                        aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATgg                                                                                                                      >  2:186625/1‑149 (MQ=255)
                         ccGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgt                                                                                                                     <  2:2254744/149‑1 (MQ=255)
                          cGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtc                                                                                                                    >  1:3494302/1‑149 (MQ=255)
                          cGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtc                                                                                                                    >  1:3494249/1‑149 (MQ=255)
                             aaTGTCGTTATTCGCGCTTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                 <  1:2487836/149‑1 (MQ=255)
                             aaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                 >  1:2047408/1‑149 (MQ=255)
                             aaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                 <  2:3682375/149‑1 (MQ=255)
                              aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCa                                                                                                                <  2:1555763/149‑1 (MQ=255)
                                 tCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGc                                                                                                             >  1:3429054/1‑149 (MQ=255)
                                    ttATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCa                                                                                                          >  1:1165053/1‑149 (MQ=255)
                                     tATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAc                                                                                                         >  2:3219229/1‑149 (MQ=255)
                                         cgcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAtt                                                                                                     <  1:233326/149‑1 (MQ=255)
                                          gcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTa                                                                                                    >  2:3658704/1‑149 (MQ=255)
                                              gTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACTATGTGCGTCACGATGGTCGTCACGCCCACGATTATGAc                                                                                                <  2:1103584/149‑1 (MQ=255)
                                               tataAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACt                                                                                               >  1:332327/1‑149 (MQ=255)
                                                ataAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTa                                                                                              <  1:124901/149‑1 (MQ=255)
                                                ataAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTa                                                                                              <  1:2202215/149‑1 (MQ=255)
                                                 taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAt                                                                                             <  2:1897169/149‑1 (MQ=255)
                                                 taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAt                                                                                             <  2:3934618/149‑1 (MQ=255)
                                                        aGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAcc                                                                                      >  1:2848491/1‑149 (MQ=255)
                                                        aGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAcc                                                                                      <  2:664807/149‑1 (MQ=255)
                                                          aCGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcgc                                                                                    >  2:2073789/1‑149 (MQ=255)
                                                            ggCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGt                                                                                  <  2:677165/149‑1 (MQ=255)
                                                               aaaCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCg                                                                               >  2:3357184/1‑149 (MQ=255)
                                                                aaCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGt                                                                              >  1:3946770/1‑149 (MQ=255)
                                                                  cTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTCTGTCCACCGCGTTCGTGa                                                                            <  1:396021/149‑1 (MQ=255)
                                                                  cTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGa                                                                            <  1:3669619/149‑1 (MQ=255)
                                                                    gACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGata                                                                          >  2:3315897/1‑149 (MQ=255)
                                                                    gACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGata                                                                          >  2:950657/1‑149 (MQ=255)
                                                                      cGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATAtt                                                                        <  2:1679573/149‑1 (MQ=255)
                                                                        cTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGa                                                                      <  1:3306114/149‑1 (MQ=255)
                                                                         tGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGaa                                                                     <  1:466980/149‑1 (MQ=255)
                                                                         tGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGaa                                                                     <  2:2566735/149‑1 (MQ=255)
                                                                          ggTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAg                                                                    <  2:1039635/149‑1 (MQ=255)
                                                                          ggTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAg                                                                    >  1:4017955/1‑149 (MQ=255)
                                                                            tGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGcc                                                                  >  2:1595808/1‑149 (MQ=255)
                                                                             gAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCg                                                                 >  1:818275/1‑149 (MQ=255)
                                                                                  aTCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACg                                                            >  2:153134/1‑149 (MQ=255)
                                                                                    cTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGcc                                                          >  1:2472089/1‑149 (MQ=255)
                                                                                           ttGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGt                                                   <  2:2595098/149‑1 (MQ=255)
                                                                                           ttGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGt                                                   >  2:2722125/1‑149 (MQ=255)
                                                                                            tGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTTATATTGAAGCAGACACGCCCGCACGtt                                                  <  1:1400966/149‑1 (MQ=255)
                                                                                             gATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTta                                                 >  2:4024530/1‑149 (MQ=255)
                                                                                              aTCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTtat                                                >  1:2364190/1‑149 (MQ=255)
                                                                                                cTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATaa                                              <  1:2573177/149‑1 (MQ=255)
                                                                                                cTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATaa                                              <  1:1174092/149‑1 (MQ=255)
                                                                                                   ggCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGc                                           <  2:3746791/149‑1 (MQ=255)
                                                                                                       aaaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGAt                                       <  2:2455679/149‑1 (MQ=255)
                                                                                                       aaaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGAt                                       <  2:2879041/149‑1 (MQ=255)
                                                                                                        aaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                      <  1:3658704/149‑1 (MQ=255)
                                                                                                        aaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                      >  1:2031677/1‑149 (MQ=255)
                                                                                                         aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                     >  2:2124605/1‑149 (MQ=255)
                                                                                                         aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                     >  2:413156/1‑149 (MQ=255)
                                                                                                         aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                     >  2:2318083/1‑149 (MQ=255)
                                                                                                         aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                     >  2:4097190/1‑149 (MQ=255)
                                                                                                           gCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTg                                   <  2:818275/149‑1 (MQ=255)
                                                                                                           gCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTg                                   >  2:2470895/1‑149 (MQ=255)
                                                                                                            ccGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                  <  2:1056685/149‑1 (MQ=255)
                                                                                                            ccGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                  <  1:2114820/149‑1 (MQ=255)
                                                                                                             cGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGAt                                 <  2:3020120/149‑1 (MQ=255)
                                                                                                                 gCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGt                             <  2:2364190/149‑1 (MQ=255)
                                                                                                                   aGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTa                           <  2:3431378/149‑1 (MQ=255)
                                                                                                                    gATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                          >  1:3400685/1‑149 (MQ=255)
                                                                                                                     aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                         <  1:3177225/149‑1 (MQ=255)
                                                                                                                     aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                         >  1:1217456/1‑149 (MQ=255)
                                                                                                                     aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                         <  1:3087638/149‑1 (MQ=255)
                                                                                                                       ccTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                       >  1:1697433/1‑149 (MQ=255)
                                                                                                                        cTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGa                      >  2:4094087/1‑149 (MQ=255)
                                                                                                                         ttAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGaa                     >  1:2041661/1‑149 (MQ=255)
                                                                                                                         ttAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGaa                     >  2:274236/1‑149 (MQ=255)
                                                                                                                           aGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtc                   >  2:1436635/1‑149 (MQ=255)
                                                                                                                               aGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtcttct               <  1:808104/149‑1 (MQ=255)
                                                                                                                               aGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtcttct               >  1:3331533/1‑149 (MQ=255)
                                                                                                                                gCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTg              >  2:1706044/1‑149 (MQ=255)
                                                                                                                                  tGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGt            <  1:2045326/149‑1 (MQ=255)
                                                                                                                                      tgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcg        >  1:1524756/1‑149 (MQ=255)
                                                                                                                                       gatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc       <  2:710377/149‑1 (MQ=255)
                                                                                                                                       gatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc       >  1:3600793/1‑149 (MQ=255)
                                                                                                                                       gatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc       <  1:3264982/149‑1 (MQ=255)
                                                                                                                                        atgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGcc      <  1:3295414/149‑1 (MQ=255)
                                                                                                                                           atCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCggg   >  2:1263540/1‑149 (MQ=255)
                                                                                                                                            tCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGa  >  2:3601013/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGA  >  CP009273/2216150‑2216438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG  >  CP009273/2216148‑2216443
                                                                                                                                                    |                                                                                                                                                   
CGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAA                                                                                                                                                    >  SRR19973068.3204353/1‑150 (MQ=60)
  CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAA                                                                                                                                                  <  SRR19973068.429851/150‑1 (MQ=60)
   TGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAG                                                                                                                                                 <  SRR19973068.3641875/150‑1 (MQ=60)
   TGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAG                                                                                                                                                 <  SRR19973068.2999888/150‑1 (MQ=60)
    GGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGA                                                                                                                                                <  SRR19973068.1387601/150‑1 (MQ=60)
    GGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGA                                                                                                                                                <  SRR19973068.1387622/150‑1 (MQ=60)
    GGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGA                                                                                                                                                >  SRR19973068.228070/1‑150 (MQ=60)
    GGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGA                                                                                                                                                >  SRR19973068.2316185/1‑150 (MQ=60)
      TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                              >  SRR19973068.2794837/1‑150 (MQ=60)
      TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                              >  SRR19973068.2794840/1‑150 (MQ=60)
      TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                              >  SRR19973068.934462/1‑150 (MQ=60)
      TGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATG                                                                                                                                              >  SRR19973068.2794826/1‑150 (MQ=60)
        CAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAC                                                                                                                                            >  SRR19973068.4031230/1‑150 (MQ=60)
         AACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACG                                                                                                                                           <  SRR19973068.550892/150‑1 (MQ=60)
           CGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAT                                                                                                                                         <  SRR19973068.764621/150‑1 (MQ=60)
            GTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATG                                                                                                                                        <  SRR19973068.1022714/150‑1 (MQ=60)
                CCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCG                                                                                                                                    <  SRR19973068.1951845/150‑1 (MQ=60)
                CCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCG                                                                                                                                    <  SRR19973068.198208/150‑1 (MQ=60)
                  CGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTC                                                                                                                                  >  SRR19973068.1780875/1‑150 (MQ=60)
                   GGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCA                                                                                                                                 <  SRR19973068.1216668/150‑1 (MQ=60)
                         TACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGG                                                                                                                           <  SRR19973068.2585443/150‑1 (MQ=60)
                           CCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                         >  SRR19973068.3515532/1‑150 (MQ=60)
                           CCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                         >  SRR19973068.1442910/1‑150 (MQ=60)
                           CCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                         >  SRR19973068.3515493/1‑150 (MQ=60)
                           CCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                         >  SRR19973068.3515479/1‑150 (MQ=60)
                              AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                      >  SRR19973068.2060716/1‑150 (MQ=60)
                              AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                      >  SRR19973068.2060759/1‑150 (MQ=60)
                               AATGTCGTTATTCGCGCTTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCA                                                                                                                     <  SRR19973068.2503703/150‑1 (MQ=60)
                                  GTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGC                                                                                                                  >  SRR19973068.3449713/1‑150 (MQ=60)
                                   TCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCC                                                                                                                 <  SRR19973068.914957/150‑1 (MQ=60)
                                    CGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCC                                                                                                                >  SRR19973068.3432704/1‑150 (MQ=60)
                                    CGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCC                                                                                                                <  SRR19973068.2322938/150‑1 (MQ=60)
                                     GTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCA                                                                                                               >  SRR19973068.1172835/1‑150 (MQ=60)
                                     GTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCA                                                                                                               >  SRR19973068.3518495/1‑150 (MQ=60)
                                     GTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCA                                                                                                               >  SRR19973068.1174291/1‑150 (MQ=60)
                                     GTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCA                                                                                                               <  SRR19973068.2271407/150‑1 (MQ=60)
                                     GTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCAGAAAAGATGACGATGTGCGTCACGAGGGGCGTCACGCCCA                                                                                                               >  SRR19973068.3487931/1‑150 (MQ=60)
                                           CGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTA                                                                                                         <  SRR19973068.2474634/150‑1 (MQ=60)
                                           CGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTA                                                                                                         <  SRR19973068.235056/150‑1 (MQ=60)
                                             CGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATG                                                                                                       >  SRR19973068.455506/1‑150 (MQ=60)
                                                GTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACT                                                                                                    >  SRR19973068.523927/1‑150 (MQ=60)
                                                GTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACT                                                                                                    >  SRR19973068.334444/1‑150 (MQ=60)
                                                  ATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAT                                                                                                  <  SRR19973068.126063/150‑1 (MQ=60)
                                                  ATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAT                                                                                                  <  SRR19973068.126024/150‑1 (MQ=60)
                                                  ATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAT                                                                                                  <  SRR19973068.2216604/150‑1 (MQ=60)
                                                    AAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGT                                                                                                >  SRR19973068.967183/1‑150 (MQ=60)
                                                    AAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGT                                                                                                >  SRR19973068.3038710/1‑150 (MQ=60)
                                                     AATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTC                                                                                               <  SRR19973068.906789/150‑1 (MQ=60)
                                                      ATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCC                                                                                              <  SRR19973068.349082/150‑1 (MQ=60)
                                                      ATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCC                                                                                              <  SRR19973068.2470945/150‑1 (MQ=60)
                                                      ATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCC                                                                                              >  SRR19973068.773401/1‑150 (MQ=60)
                                                         AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                           >  SRR19973068.2866236/1‑150 (MQ=60)
                                                                 AAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGT                                                                                   >  SRR19973068.3970663/1‑150 (MQ=60)
                                                                    CTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTCTGTCCACCGCGTTCGTGAT                                                                                <  SRR19973068.398787/150‑1 (MQ=60)
                                                                    CTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGAT                                                                                <  SRR19973068.3691775/150‑1 (MQ=60)
                                                                    CTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGAT                                                                                <  SRR19973068.3691796/150‑1 (MQ=60)
                                                                     TGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATA                                                                               >  SRR19973068.1607241/1‑150 (MQ=60)
                                                                      GACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATAT                                                                              <  SRR19973068.205121/150‑1 (MQ=60)
                                                                          CTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAA                                                                          <  SRR19973068.3326340/150‑1 (MQ=60)
                                                                          CTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAA                                                                          <  SRR19973068.3327187/150‑1 (MQ=60)
                                                                           TGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAG                                                                         >  SRR19973068.4042206/1‑150 (MQ=60)
                                                                           TGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAG                                                                         <  SRR19973068.2366536/150‑1 (MQ=60)
                                                                           TGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAG                                                                         <  SRR19973068.470272/150‑1 (MQ=60)
                                                                            GGTGAAACATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCGACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGC                                                                        <  SRR19973068.416392/150‑1 (MQ=60)
                                                                             GTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCC                                                                       <  SRR19973068.423064/150‑1 (MQ=60)
                                                                              TGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCG                                                                      >  SRR19973068.823837/1‑150 (MQ=60)
                                                                                AACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGAC                                                                    <  SRR19973068.622167/150‑1 (MQ=60)
                                                                                     TCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCC                                                               >  SRR19973068.2487864/1‑150 (MQ=60)
                                                                                     TCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCC                                                               >  SRR19973068.2054081/1‑150 (MQ=60)
                                                                                     TCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGGCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCC                                                               >  SRR19973068.2487867/1‑150 (MQ=60)
                                                                                           GATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACG                                                         <  SRR19973068.3780806/150‑1 (MQ=60)
                                                                                           GATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACG                                                         <  SRR19973068.1725452/150‑1 (MQ=60)
                                                                                              TGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTTATATTGAAGCAGACACGCCCGCACGTTA                                                      <  SRR19973068.1409952/150‑1 (MQ=60)
                                                                                              TGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTA                                                      >  SRR19973068.2804404/1‑150 (MQ=60)
                                                                                              TGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCAGACACGCCCGCACGTTA                                                      <  SRR19973068.3970985/150‑1 (MQ=60)
                                                                                               GATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTAT                                                     >  SRR19973068.2379318/1‑150 (MQ=60)
                                                                                                ATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATA                                                    >  SRR19973068.2181518/1‑150 (MQ=60)
                                                                                                  CTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACTATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCACGCACGTTATAAC                                                  >  SRR19973068.1716763/1‑150 (MQ=60)
                                                                                                  CTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAAC                                                  <  SRR19973068.1181946/150‑1 (MQ=60)
                                                                                                  CTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAAC                                                  <  SRR19973068.1182012/150‑1 (MQ=60)
                                                                                                  CTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAAC                                                  <  SRR19973068.2589462/150‑1 (MQ=60)
                                                                                                         AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                           >  SRR19973068.2044980/1‑150 (MQ=60)
                                                                                                          AACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCC                                          <  SRR19973068.3680836/150‑1 (MQ=60)
                                                                                                          AACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCC                                          <  SRR19973068.3680815/150‑1 (MQ=60)
                                                                                                            CGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTG                                        >  SRR19973068.2064757/1‑150 (MQ=60)
                                                                                                              CCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGAT                                      <  SRR19973068.2128625/150‑1 (MQ=60)
                                                                                                               CGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATC                                     <  SRR19973068.2794011/150‑1 (MQ=60)
                                                                                                                GGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCG                                    <  SRR19973068.319675/150‑1 (MQ=60)
                                                                                                                  AGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATagatcggaag                                  >  SRR19973068.2078863/1‑140 (MQ=60)
                                                                                                                  AGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGT                                  >  SRR19973068.3346062/1‑150 (MQ=60)
                                                                                                                  AGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGT                                  >  SRR19973068.3344420/1‑150 (MQ=60)
                                                                                                                     AGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAT                               >  SRR19973068.3421261/1‑150 (MQ=60)
                                                                                                                      GATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATT                              >  SRR19973068.1225560/1‑150 (MQ=60)
                                                                                                                       ATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTT                             <  SRR19973068.3196776/150‑1 (MQ=60)
                                                                                                                       ATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTT                             <  SRR19973068.3106442/150‑1 (MQ=60)
                                                                                                                        TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                            >  SRR19973068.1708302/1‑150 (MQ=60)
                                                                                                                          CTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAA                          >  SRR19973068.2054992/1‑150 (MQ=60)
                                                                                                                          CTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAA                          >  SRR19973068.2055457/1‑150 (MQ=60)
                                                                                                                            TAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATC                        <  SRR19973068.1073839/150‑1 (MQ=60)
                                                                                                                             AGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCT                       >  SRR19973068.141035/1‑150 (MQ=60)
                                                                                                                             AGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCT                       >  SRR19973068.141259/1‑150 (MQ=60)
                                                                                                                              GCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTT                      <  SRR19973068.1848671/150‑1 (MQ=60)
                                                                                                                               CAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTC                     >  SRR19973068.3220335/1‑150 (MQ=60)
                                                                                                                                AAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCT                    >  SRR19973068.3351865/1‑150 (MQ=60)
                                                                                                                                 AGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTG                   <  SRR19973068.816849/150‑1 (MQ=60)
                                                                                                                                 AGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTG                   <  SRR19973068.813603/150‑1 (MQ=60)
                                                                                                                                 AGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTG                   <  SRR19973068.3709294/150‑1 (MQ=60)
                                                                                                                                    TGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTT                <  SRR19973068.2058668/150‑1 (MQ=60)
                                                                                                                                    TGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTT                <  SRR19973068.2757042/150‑1 (MQ=60)
                                                                                                                                    TGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTT                <  SRR19973068.723649/150‑1 (MQ=60)
                                                                                                                                       ATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCG             >  SRR19973068.1534781/1‑150 (MQ=60)
                                                                                                                                        TGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGC            >  SRR19973068.3622663/1‑150 (MQ=60)
                                                                                                                                         GATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCC           >  SRR19973068.3405881/1‑150 (MQ=60)
                                                                                                                                         GATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCC           <  SRR19973068.3285027/150‑1 (MQ=60)
                                                                                                                                         GATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCC           <  SRR19973068.3285473/150‑1 (MQ=60)
                                                                                                                                          ATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCG          <  SRR19973068.3315602/150‑1 (MQ=60)
                                                                                                                                          ATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCG          <  SRR19973068.3315515/150‑1 (MQ=60)
                                                                                                                                          ATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCG          <  SRR19973068.3315635/150‑1 (MQ=60)
                                                                                                                                                 CATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT   >  SRR19973068.2111666/1‑150 (MQ=60)
                                                                                                                                                 CATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT   >  SRR19973068.2952065/1‑150 (MQ=60)
                                                                                                                                                  ATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG  <  SRR19973068.1493582/150‑1 (MQ=60)
                                                                                                                                                  ATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG  <  SRR19973068.1985925/150‑1 (MQ=60)
                                                                                                                                                  ATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG  <  SRR19973068.3150039/150‑1 (MQ=60)
                                                                                                                                                  ATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG  <  SRR19973068.2122448/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTG  >  CP009273/2216148‑2216443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑