Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,216,296 C→A 100% I211I (ATC→ATA)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,216,2960CA96.0% 797.1 / 18.1 235I211I (ATC→ATA) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base C (2/8);  new base A (110/115);  total (112/123)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 237 total aligned reads displayed.

GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  >  CP009273/2216149‑2216442
                                                                                                                                                   |                                                                                                                                                  
 cTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATaaaa                                                                                                                                                 >  1:1027401/1‑150 (MQ=255)
    gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAt                                                                                                                                              <  2:913914/150‑1 (MQ=255)
    gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAt                                                                                                                                              <  1:3461031/150‑1 (MQ=255)
    gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAt                                                                                                                                              <  1:1460110/150‑1 (MQ=255)
    gTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAt                                                                                                                                              <  2:781402/150‑1 (MQ=255)
     tGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                             <  2:3804283/150‑1 (MQ=255)
       cAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAc                                                                                                                                           <  2:1928176/150‑1 (MQ=255)
       cAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAc                                                                                                                                           >  2:102401/1‑150 (MQ=255)
       cAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAc                                                                                                                                           <  1:2760876/150‑1 (MQ=255)
        aaCGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACg                                                                                                                                          <  2:1320701/150‑1 (MQ=255)
             ggCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAtgtg                                                                                                                                     <  1:4150691/150‑1 (MQ=255)
                  ggCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCa                                                                                                                                >  2:1183386/1‑150 (MQ=255)
                  ggCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCa                                                                                                                                >  1:2794133/1‑150 (MQ=255)
                    cGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACg                                                                                                                              >  2:688148/1‑150 (MQ=255)
                     gTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGa                                                                                                                             >  2:3549816/1‑150 (MQ=255)
                     gTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGa                                                                                                                             >  2:2899756/1‑150 (MQ=255)
                        taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCTTCACGATgg                                                                                                                          <  1:4027098/150‑1 (MQ=255)
                        taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATgg                                                                                                                          <  1:1796395/150‑1 (MQ=255)
                        taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATgg                                                                                                                          >  1:1876244/1‑150 (MQ=255)
                         aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgt                                                                                                                         <  2:1611062/150‑1 (MQ=255)
                         aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgt                                                                                                                         <  1:2093733/150‑1 (MQ=255)
                           cGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcg                                                                                                                       >  1:1176541/1‑150 (MQ=255)
                            gAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgt                                                                                                                      >  2:847663/1‑150 (MQ=255)
                            gAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgt                                                                                                                      >  2:3022982/1‑150 (MQ=255)
                              aaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCa                                                                                                                    <  1:3184610/150‑1 (MQ=255)
                               aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCAc                                                                                                                   >  2:3551588/1‑150 (MQ=255)
                                tGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACg                                                                                                                  >  2:328059/1‑150 (MQ=255)
                                   cGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGccc                                                                                                               >  2:1763495/1‑150 (MQ=255)
                                   cGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGccc                                                                                                               >  2:1131266/1‑150 (MQ=255)
                                     ttATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAc                                                                                                             <  1:3089847/150‑1 (MQ=255)
                                         tCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCTTCACGATGGTCGTCACGCCCACGAtt                                                                                                         <  2:3857370/150‑1 (MQ=255)
                                         tCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAtt                                                                                                         <  1:1100899/150‑1 (MQ=255)
                                         tCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAtt                                                                                                         <  2:892517/150‑1 (MQ=255)
                                               gTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACt                                                                                                   >  2:2487093/1‑150 (MQ=255)
                                                 ataAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAt                                                                                                 <  1:2699723/150‑1 (MQ=255)
                                                 ataAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAt                                                                                                 <  2:3396238/150‑1 (MQ=255)
                                                    aaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTc                                                                                              <  2:2961248/150‑1 (MQ=255)
                                                       gAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAc                                                                                           <  1:641554/150‑1 (MQ=255)
                                                       gAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAc                                                                                           >  2:1813594/1‑150 (MQ=255)
                                                        aaGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAcc                                                                                          >  1:2915844/1‑150 (MQ=255)
                                                         aGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcg                                                                                         >  2:283666/1‑150 (MQ=255)
                                                           aCGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcgcg                                                                                       >  1:1533369/1‑150 (MQ=255)
                                                             ggCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGtt                                                                                     >  2:3756665/1‑150 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                    <  2:313633/150‑1 (MQ=255)
                                                               cAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCg                                                                                   >  1:3364492/1‑150 (MQ=255)
                                                               cAAACTGACGCTGGAGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCg                                                                                   >  1:130683/1‑150 (MQ=255)
                                                                aaccTGACGCTGGTGAACCATCGGGGGAGTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGCTGGTCGTCACGCCCACTATTATGACTATGTCCACCGCGTTCGt                                                                                  <  2:3463461/147‑1 (MQ=255)
                                                                aaaCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGt                                                                                  <  2:2400087/150‑1 (MQ=255)
                                                                  aCTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGa                                                                                <  1:4311588/150‑1 (MQ=255)
                                                                    tGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGata                                                                              >  2:4232667/1‑150 (MQ=255)
                                                                          tGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAg                                                                        <  2:614258/150‑1 (MQ=255)
                                                                           ggTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGc                                                                       >  2:2193366/1‑150 (MQ=255)
                                                                            gTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGcc                                                                      <  2:1631992/150‑1 (MQ=255)
                                                                             tGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCg                                                                     >  2:3296137/1‑150 (MQ=255)
                                                                             tGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCg                                                                     >  2:2288651/1‑150 (MQ=255)
                                                                             tGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCg                                                                     <  1:1366353/150‑1 (MQ=255)
                                                                               aaCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGac                                                                   >  2:3378244/1‑150 (MQ=255)
                                                                               aaCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGac                                                                   >  2:2600930/1‑150 (MQ=255)
                                                                                aCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGtca                                                                  <  2:781271/150‑1 (MQ=255)
                                                                                      tGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCg                                                            <  2:3582641/150‑1 (MQ=255)
                                                                                         ggATTGATCTGGGCTAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCAc                                                         <  1:3275909/150‑1 (MQ=255)
                                                                                         ggATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCAc                                                         <  2:1150494/150‑1 (MQ=255)
                                                                                              gATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTtat                                                    <  2:1476155/150‑1 (MQ=255)
                                                                                                tCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATaa                                                  <  2:2886137/150‑1 (MQ=255)
                                                                                                 cTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAAc                                                 <  2:3387887/150‑1 (MQ=255)
                                                                                                  tGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACg                                                >  1:400388/1‑150 (MQ=255)
                                                                                                    ggCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGcc                                              <  1:974134/150‑1 (MQ=255)
                                                                                                        aaaCGCCGGAGCAGATCCTTAGCAAGCTGTATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                          <  1:2583488/150‑1 (MQ=255)
                                                                                                        aaaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATc                                          <  1:3106462/150‑1 (MQ=255)
                                                                                                         aaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                         <  2:4125576/150‑1 (MQ=255)
                                                                                                            gCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGa                                      >  1:125030/1‑150 (MQ=255)
                                                                                                             ccGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGAt                                     <  2:2686759/150‑1 (MQ=255)
                                                                                                             ccGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGAt                                     >  2:317709/1‑150 (MQ=255)
                                                                                                              cGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATc                                    <  2:3921034/150‑1 (MQ=255)
                                                                                                                gAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCgg                                  <  1:3515644/150‑1 (MQ=255)
                                                                                                                 aGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGt                                 <  1:1662871/150‑1 (MQ=255)
                                                                                                                   cAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTa                               <  1:688148/150‑1 (MQ=255)
                                                                                                                     gATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                             <  2:2550450/150‑1 (MQ=255)
                                                                                                                     gATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                             >  2:522144/1‑150 (MQ=255)
                                                                                                                     gATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                             >  2:4224944/1‑150 (MQ=255)
                                                                                                                      aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAttt                            >  2:3998737/1‑150 (MQ=255)
                                                                                                                       tCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                           >  1:2145040/1‑150 (MQ=255)
                                                                                                                       tCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                           <  2:3791577/150‑1 (MQ=255)
                                                                                                                       tCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTg                           >  1:3306175/1‑150 (MQ=255)
                                                                                                                         cTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGaa                         >  2:2313697/1‑150 (MQ=255)
                                                                                                                            aGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCTTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtct                      <  2:3369847/150‑1 (MQ=255)
                                                                                                                             gCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtctt                     <  1:2962220/150‑1 (MQ=255)
                                                                                                                                aGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTg                  <  2:304558/150‑1 (MQ=255)
                                                                                                                                 gCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTgg                 <  1:3041781/150‑1 (MQ=255)
                                                                                                                                   tGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGtt               >  1:725787/1‑150 (MQ=255)
                                                                                                                                      atgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcg            >  2:2110929/1‑150 (MQ=255)
                                                                                                                                       tgatgatCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc           >  2:1227241/1‑150 (MQ=255)
                                                                                                                                       tgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc           >  1:3316697/1‑150 (MQ=255)
                                                                                                                                       tgatgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTgcgc           >  1:2465769/1‑150 (MQ=255)
                                                                                                                                           gatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCggg       >  2:2534514/1‑150 (MQ=255)
                                                                                                                                           gatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCggg       >  1:3926804/1‑150 (MQ=255)
                                                                                                                                           gatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCggg       <  1:2584558/150‑1 (MQ=255)
                                                                                                                                              cGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAg    >  1:1826476/1‑150 (MQ=255)
                                                                                                                                                cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCt  >  1:368572/1‑150 (MQ=255)
                                                                                                                                                cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCt  <  1:835615/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  >  CP009273/2216149‑2216442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGG  >  CP009273/2216148‑2216444
                                                                                                                                                    |                                                                                                                                                    
CGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAA                                                                                                                                                     <  SRR19973079.1983911/150‑1 (MQ=60)
 GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAA                                                                                                                                                    >  SRR19973079.3387201/1‑150 (MQ=60)
  CTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAA                                                                                                                                                   >  SRR19973079.1033123/1‑150 (MQ=60)
     GTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAT                                                                                                                                                <  SRR19973079.1468055/150‑1 (MQ=60)
     GTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGAT                                                                                                                                                <  SRR19973079.3478890/150‑1 (MQ=60)
        CAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAC                                                                                                                                             <  SRR19973079.2775095/150‑1 (MQ=60)
         AACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACG                                                                                                                                            <  SRR19973079.3213167/150‑1 (MQ=60)
             TGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGT                                                                                                                                        <  SRR19973079.2740670/150‑1 (MQ=60)
              GGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTG                                                                                                                                       <  SRR19973079.4171763/150‑1 (MQ=60)
                   GGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCA                                                                                                                                  >  SRR19973079.2808378/1‑150 (MQ=60)
                   GGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCA                                                                                                                                  >  SRR19973079.2808456/1‑150 (MQ=60)
                   GGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCA                                                                                                                                  >  SRR19973079.2808459/1‑150 (MQ=60)
                         TACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCTTCACGATGG                                                                                                                            <  SRR19973079.4047422/150‑1 (MQ=60)
                         TACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCTTCACGATGG                                                                                                                            <  SRR19973079.4130493/150‑1 (MQ=60)
                         TACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGG                                                                                                                            >  SRR19973079.1886217/1‑150 (MQ=60)
                         TACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGG                                                                                                                            <  SRR19973079.1805757/150‑1 (MQ=60)
                          ACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGT                                                                                                                           <  SRR19973079.4301868/150‑1 (MQ=60)
                          ACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGT                                                                                                                           <  SRR19973079.2104629/150‑1 (MQ=60)
                          ACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGT                                                                                                                           <  SRR19973079.2944837/150‑1 (MQ=60)
                           CCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                          >  SRR19973079.1228231/1‑150 (MQ=60)
                           CCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTC                                                                                                                          >  SRR19973079.3432519/1‑150 (MQ=60)
                            CGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCG                                                                                                                         >  SRR19973079.1183317/1‑150 (MQ=60)
                              AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                       >  SRR19973079.1337754/1‑150 (MQ=60)
                              AAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTC                                                                                                                       >  SRR19973079.703203/1‑150 (MQ=60)
                               AATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCA                                                                                                                      <  SRR19973079.3200888/150‑1 (MQ=60)
                                      TTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAC                                                                                                               <  SRR19973079.3105823/150‑1 (MQ=60)
                                      TTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAC                                                                                                               <  SRR19973079.400088/150‑1 (MQ=60)
                                      TTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGAGCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAC                                                                                                               <  SRR19973079.3105846/150‑1 (MQ=60)
                                          TCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATT                                                                                                           <  SRR19973079.1107067/150‑1 (MQ=60)
                                          TCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATT                                                                                                           <  SRR19973079.2024625/150‑1 (MQ=60)
                                           CGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTA                                                                                                          <  SRR19973079.1569238/150‑1 (MQ=60)
                                             CGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATG                                                                                                        >  SRR19973079.731165/1‑150 (MQ=60)
                                                GTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACT                                                                                                     >  SRR19973079.669992/1‑150 (MQ=60)
                                                  ATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAT                                                                                                   >  SRR19973079.1861807/1‑150 (MQ=60)
                                                  ATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTAT                                                                                                   <  SRR19973079.2713701/150‑1 (MQ=60)
                                                    AAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGT                                                                                                 >  SRR19973079.1690663/1‑150 (MQ=60)
                                                       TGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCA                                                                                              >  SRR19973079.4056183/1‑150 (MQ=60)
                                                       TGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCA                                                                                              <  SRR19973079.8602/150‑1 (MQ=60)
                                                        GAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAC                                                                                             <  SRR19973079.645201/150‑1 (MQ=60)
                                                         AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                            >  SRR19973079.2930641/1‑150 (MQ=60)
                                                         AAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACC                                                                                            <  SRR19973079.3086488/150‑1 (MQ=60)
                                                          AGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCG                                                                                           <  SRR19973079.3181513/150‑1 (MQ=60)
                                                            ACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCG                                                                                         >  SRR19973079.1541657/1‑150 (MQ=60)
                                                            ACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCG                                                                                         >  SRR19973079.1541827/1‑150 (MQ=60)
                                                              GGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTT                                                                                       >  SRR19973079.2927649/1‑150 (MQ=60)
                                                              GGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTT                                                                                       >  SRR19973079.3486996/1‑150 (MQ=60)
                                                               GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                      <  SRR19973079.3026763/150‑1 (MQ=60)
                                                               GCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTC                                                                                      <  SRR19973079.3026769/150‑1 (MQ=60)
                                                                CAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCG                                                                                     >  SRR19973079.3381807/1‑150 (MQ=60)
                                                                CAAACTGACGCTGGAGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCG                                                                                     >  SRR19973079.131747/1‑150 (MQ=60)
                                                                   ACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGA                                                                                  <  SRR19973079.4333299/150‑1 (MQ=60)
                                                                   ACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGA                                                                                  <  SRR19973079.1791465/150‑1 (MQ=60)
                                                                    CTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGAT                                                                                 <  SRR19973079.1094687/150‑1 (MQ=60)
                                                                      GACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATAT                                                                               <  SRR19973079.3177583/150‑1 (MQ=60)
                                                                          CTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAA                                                                           >  SRR19973079.3339654/1‑150 (MQ=60)
                                                                           TGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAG                                                                          >  SRR19973079.2411393/1‑150 (MQ=60)
                                                                              TGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCG                                                                       <  SRR19973079.1373943/150‑1 (MQ=60)
                                                                                        GGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGC                                                             >  SRR19973079.76966/1‑150 (MQ=60)
                                                                                          GGATTGATCTGGGCTAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCAC                                                           <  SRR19973079.3292485/150‑1 (MQ=60)
                                                                                          GGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCAC                                                           <  SRR19973079.1808130/150‑1 (MQ=60)
                                                                                                   TGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACG                                                  >  SRR19973079.402720/1‑150 (MQ=60)
                                                                                                   TGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACG                                                  >  SRR19973079.402980/1‑150 (MQ=60)
                                                                                                     GGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCC                                                <  SRR19973079.979668/150‑1 (MQ=60)
                                                                                                     GGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCC                                                <  SRR19973079.979682/150‑1 (MQ=60)
                                                                                                       CGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGA                                              >  SRR19973079.1878335/1‑150 (MQ=60)
                                                                                                         AAACGCCGGAGCATATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACTATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                            <  SRR19973079.2596909/150‑1 (MQ=60)
                                                                                                         AAACGCCGGAGCAGATCCTTAGCAAGCTGTATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                            <  SRR19973079.2596717/150‑1 (MQ=60)
                                                                                                         AAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATC                                            <  SRR19973079.3122495/150‑1 (MQ=60)
                                                                                                             GCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGA                                        >  SRR19973079.126031/1‑150 (MQ=60)
                                                                                                             GCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGA                                        >  SRR19973079.2328476/1‑150 (MQ=60)
                                                                                                             GCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGA                                        >  SRR19973079.1266831/1‑150 (MQ=60)
                                                                                                             GCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGA                                        >  SRR19973079.126066/1‑150 (MQ=60)
                                                                                                             GCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACGATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTGATAACGCCGATCCTGA                                        >  SRR19973079.141406/1‑150 (MQ=60)
                                                                                                                 GAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGG                                    <  SRR19973079.3533715/150‑1 (MQ=60)
                                                                                                                  AGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGT                                   <  SRR19973079.1671880/150‑1 (MQ=60)
                                                                                                                  AGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGT                                   >  SRR19973079.3563031/1‑150 (MQ=60)
                                                                                                                    CAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTA                                 <  SRR19973079.691910/150‑1 (MQ=60)
                                                                                                                    CAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTA                                 >  SRR19973079.2534976/1‑150 (MQ=60)
                                                                                                                       ATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTT                              >  SRR19973079.542074/1‑150 (MQ=60)
                                                                                                                        TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                             <  SRR19973079.645165/150‑1 (MQ=60)
                                                                                                                        TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                             >  SRR19973079.2154631/1‑150 (MQ=60)
                                                                                                                        TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                             >  SRR19973079.2156066/1‑150 (MQ=60)
                                                                                                                        TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                             >  SRR19973079.3323045/1‑150 (MQ=60)
                                                                                                                        TCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTG                             >  SRR19973079.3323051/1‑150 (MQ=60)
                                                                                                                              GCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTT                       <  SRR19973079.2977439/150‑1 (MQ=60)
                                                                                                                               CAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTC                      <  SRR19973079.3783569/150‑1 (MQ=60)
                                                                                                                                  GCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGG                   <  SRR19973079.3057535/150‑1 (MQ=60)
                                                                                                                                    TGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTT                 >  SRR19973079.3714189/1‑150 (MQ=60)
                                                                                                                                    TGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTT                 >  SRR19973079.729637/1‑150 (MQ=60)
                                                                                                                                        TGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGC             >  SRR19973079.2478383/1‑150 (MQ=60)
                                                                                                                                        TGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGC             >  SRR19973079.3333306/1‑150 (MQ=60)
                                                                                                                                        TGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGC             >  SRR19973079.3333639/1‑150 (MQ=60)
                                                                                                                                           TGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGG          >  SRR19973079.1118016/1‑150 (MQ=60)
                                                                                                                                            GATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGG         <  SRR19973079.2598287/150‑1 (MQ=60)
                                                                                                                                            GATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGG         <  SRR19973079.2597832/150‑1 (MQ=60)
                                                                                                                                            GATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGG         <  SRR19973079.2597799/150‑1 (MQ=60)
                                                                                                                                            GATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGG         >  SRR19973079.3946666/1‑150 (MQ=60)
                                                                                                                                              TCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAA       <  SRR19973079.3318104/150‑1 (MQ=60)
                                                                                                                                               CGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAG      >  SRR19973079.1836051/1‑150 (MQ=60)
                                                                                                                                                 CATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT    >  SRR19973079.142437/1‑150 (MQ=60)
                                                                                                                                                 CATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT    >  SRR19973079.370557/1‑150 (MQ=60)
                                                                                                                                                 CATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT    <  SRR19973079.840442/150‑1 (MQ=60)
                                                                                                                                                   TAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGACGCTGG  >  SRR19973079.1160370/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGG  >  CP009273/2216148‑2216444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑