Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,526,794 C→T 100% intergenic (+55/‑329) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,526,7940CT98.8% 792.4 / ‑0.2 240intergenic (+55/‑329)cysK/ptsHcysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (119/118);  total (120/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

TATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTT  >  CP009273/2526649‑2526936
                                                                                                                                                 |                                                                                                                                              
tatTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCt                                                                                                                                              >  2:616203/1‑148 (MQ=255)
tatTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCt                                                                                                                                              >  2:2381957/1‑148 (MQ=255)
 atTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTg                                                                                                                                             >  1:1124589/1‑148 (MQ=255)
 atTGTGGTTATACTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTg                                                                                                                                             >  1:2240668/1‑148 (MQ=255)
   tgtgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCt                                                                                                                                           >  2:3147994/1‑148 (MQ=255)
    gtgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCtt                                                                                                                                          <  1:1665186/148‑1 (MQ=255)
    gtgGTTATTATACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCtt                                                                                                                                          <  1:583233/148‑1 (MQ=255)
      ggTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCa                                                                                                                                        >  1:3028547/1‑148 (MQ=255)
       gTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCaa                                                                                                                                       <  2:1304315/148‑1 (MQ=255)
         tATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAAc                                                                                                                                     <  1:1620497/148‑1 (MQ=255)
                ccATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGcc                                                                                                                              >  2:100094/1‑148 (MQ=255)
                ccATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGcc                                                                                                                              >  1:651700/1‑148 (MQ=255)
                 catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGccc                                                                                                                             <  2:3190410/148‑1 (MQ=255)
                 catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTGTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGccc                                                                                                                             >  2:1664743/1‑148 (MQ=255)
                    catcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCcctc                                                                                                                          >  2:2077434/1‑148 (MQ=255)
                       cGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTg                                                                                                                       <  2:3480742/148‑1 (MQ=255)
                        gggTTAGCGTTATTTAAGCACCTCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCATCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCACCTCCTgg                                                                                                                      <  1:2825425/148‑1 (MQ=255)
                            gAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCAtt                                                                                                                  <  2:954998/148‑1 (MQ=255)
                              gCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGa                                                                                                                >  1:172450/1‑148 (MQ=255)
                                 ttATTTCAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTc                                                                                                             <  2:1874378/148‑1 (MQ=255)
                                  tATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCa                                                                                                            >  1:254029/1‑148 (MQ=255)
                                  tATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCa                                                                                                            >  2:1670124/1‑148 (MQ=255)
                                   aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                                                                                                           <  2:1450377/148‑1 (MQ=255)
                                    tttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                                                                                                          <  1:1077318/148‑1 (MQ=255)
                                    tttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                                                                                                          <  2:2858894/148‑1 (MQ=255)
                                     ttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                                         <  1:1743416/148‑1 (MQ=255)
                                     ttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                                         <  1:425546/148‑1 (MQ=255)
                                     ttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                                         <  1:3368444/148‑1 (MQ=255)
                                      tAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCt                                                                                                        <  1:1849729/148‑1 (MQ=255)
                                        aGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGt                                                                                                      <  2:1516736/148‑1 (MQ=255)
                                            cctcATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGa                                                                                                  <  1:2454761/145‑1 (MQ=255)
                                            ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGa                                                                                                  <  1:2915083/148‑1 (MQ=255)
                                             cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGaa                                                                                                 <  2:1778051/148‑1 (MQ=255)
                                               cATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                                                                                               >  1:2370057/1‑148 (MQ=255)
                                               cATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                                                                                               >  1:2792198/1‑148 (MQ=255)
                                                aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTg                                                                                              <  1:794929/148‑1 (MQ=255)
                                                aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTg                                                                                              <  1:3054779/148‑1 (MQ=255)
                                                 ttGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                                                                                             >  2:244513/1‑148 (MQ=255)
                                                  tGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGt                                                                                            >  2:3744/1‑148 (MQ=255)
                                                   gTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTa                                                                                           >  1:2328384/1‑148 (MQ=255)
                                                        ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTaa                                                                                      <  1:2673201/148‑1 (MQ=255)
                                                           aTCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCa                                                                                   >  2:447946/1‑148 (MQ=255)
                                                              tctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGAc                                                                                <  2:2943797/148‑1 (MQ=255)
                                                                 tCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa                                                                             >  2:2619872/1‑148 (MQ=255)
                                                                 tCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa                                                                             >  2:2276133/1‑148 (MQ=255)
                                                                 tCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa                                                                             <  2:1216439/148‑1 (MQ=255)
                                                                   aCTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAatt                                                                           <  1:3520309/148‑1 (MQ=255)
                                                                   aCTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAatt                                                                           <  1:3483629/148‑1 (MQ=255)
                                                                    cTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAatta                                                                          >  2:3091253/1‑148 (MQ=255)
                                                                          aagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGa                                                                    >  2:1299935/1‑148 (MQ=255)
                                                                             aaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATgc                                                                 >  2:2687299/1‑148 (MQ=255)
                                                                             aaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATgc                                                                 >  2:3300881/1‑148 (MQ=255)
                                                                               ttccAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATgcgc                                                               >  2:3311181/4‑148 (MQ=255)
                                                                                  cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaa                                                            >  2:785425/1‑148 (MQ=255)
                                                                                  cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaa                                                            >  1:667727/1‑148 (MQ=255)
                                                                                  cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaa                                                            <  1:1235639/148‑1 (MQ=255)
                                                                                   aaCAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                                           <  2:2541582/148‑1 (MQ=255)
                                                                                       gTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTaa                                                       <  2:1041329/148‑1 (MQ=255)
                                                                                        tAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAAt                                                      <  1:3277397/148‑1 (MQ=255)
                                                                                           tGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGt                                                   >  1:1714255/1‑148 (MQ=255)
                                                                                            gCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGtt                                                  <  2:3028547/148‑1 (MQ=255)
                                                                                            gCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGtt                                                  <  1:746132/148‑1 (MQ=255)
                                                                                                gCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAg                                              >  2:21248/1‑148 (MQ=255)
                                                                                                gCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAg                                              >  2:20475/1‑148 (MQ=255)
                                                                                                 cTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAgg                                             >  2:1736618/1‑148 (MQ=255)
                                                                                                  ttGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGa                                            >  2:1286077/1‑148 (MQ=255)
                                                                                                  ttGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGa                                            <  2:282978/148‑1 (MQ=255)
                                                                                                       aaaaaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGACAAGc                                       >  2:1017697/1‑148 (MQ=255)
                                                                                                       aaaaaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCGGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGc                                       <  2:1408767/148‑1 (MQ=255)
                                                                                                            tGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAg                                  <  1:3744/148‑1 (MQ=255)
                                                                                                             gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                                 <  2:3427812/148‑1 (MQ=255)
                                                                                                             gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                                 >  1:1453897/1‑148 (MQ=255)
                                                                                                             gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                                 >  1:2274147/1‑148 (MQ=255)
                                                                                                                 aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGaa                             <  2:1378622/148‑1 (MQ=255)
                                                                                                                    aaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCg                          >  2:4067/1‑148 (MQ=255)
                                                                                                                     aaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa                         >  2:363712/1‑148 (MQ=255)
                                                                                                                     aaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa                         >  2:1129642/1‑148 (MQ=255)
                                                                                                                      aGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAt                        >  1:905772/1‑148 (MQ=255)
                                                                                                                      aGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAt                        <  1:1529524/148‑1 (MQ=255)
                                                                                                                      aGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAt                        >  2:656657/1‑148 (MQ=255)
                                                                                                                        cACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAttt                      >  1:3388056/1‑148 (MQ=255)
                                                                                                                        cACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAttt                      >  2:1098839/1‑148 (MQ=255)
                                                                                                                            tttttAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAGTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg                  >  1:2598623/1‑148 (MQ=255)
                                                                                                                            tttttAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg                  <  1:1848456/148‑1 (MQ=255)
                                                                                                                             ttttAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGa                 >  1:583489/1‑148 (MQ=255)
                                                                                                                             ttttAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGa                 >  2:90204/1‑148 (MQ=255)
                                                                                                                             ttttAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTAAAAGGAAAAGCCAAAGATGAATCGATTTTATGa                 >  2:1718782/1‑148 (MQ=255)
                                                                                                                               ttAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAtt               <  2:3525015/148‑1 (MQ=255)
                                                                                                                                 aGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTg             <  1:1272095/148‑1 (MQ=255)
                                                                                                                                 aGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTg             <  2:1544507/148‑1 (MQ=255)
                                                                                                                                 aGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTg             <  1:3121604/148‑1 (MQ=255)
                                                                                                                                  ggTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTgg            <  1:1294636/148‑1 (MQ=255)
                                                                                                                                   gTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGt           <  1:616203/148‑1 (MQ=255)
                                                                                                                                    tGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGtt          >  1:2836291/1‑148 (MQ=255)
                                                                                                                                     gCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTc         >  1:27955/1‑148 (MQ=255)
                                                                                                                                     gCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTc         >  1:2490225/1‑148 (MQ=255)
                                                                                                                                      cTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCa        <  1:703445/148‑1 (MQ=255)
                                                                                                                                      cTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCa        <  2:2570000/148‑1 (MQ=255)
                                                                                                                                        tttttGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAt      >  1:1418793/1‑148 (MQ=255)
                                                                                                                                            tgtgGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAttctt  <  1:2228064/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTT  >  CP009273/2526649‑2526936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTA  >  CP009273/2526645‑2526942
                                                                                                                                                     |                                                                                                                                                    
AGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGT                                                                                                                                                      <  SRR19973066.1838435/150‑1 (MQ=60)
  AATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCT                                                                                                                                                    <  SRR19973066.3053000/150‑1 (MQ=60)
   ATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTG                                                                                                                                                   >  SRR19973066.1131853/1‑150 (MQ=60)
   ATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTG                                                                                                                                                   >  SRR19973066.1132682/1‑150 (MQ=60)
   ATATTGTGGTTATACTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTG                                                                                                                                                   >  SRR19973066.2256121/1‑150 (MQ=60)
        GTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCA                                                                                                                                              <  SRR19973066.1677317/150‑1 (MQ=60)
        GTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCA                                                                                                                                              >  SRR19973066.3048783/1‑150 (MQ=60)
        GTGGTTATTATACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCA                                                                                                                                              <  SRR19973066.587309/150‑1 (MQ=60)
        GTGGTTATTATACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCA                                                                                                                                              <  SRR19973066.587303/150‑1 (MQ=60)
          GGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAAAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAA                                                                                                                                            <  SRR19973066.650497/150‑1 (MQ=60)
          GGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAAAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAA                                                                                                                                            <  SRR19973066.650088/150‑1 (MQ=60)
            TTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACT                                                                                                                                          <  SRR19973066.783244/150‑1 (MQ=60)
             TATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTT                                                                                                                                         <  SRR19973066.1632247/150‑1 (MQ=60)
               TTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTC                                                                                                                                       <  SRR19973066.1256374/150‑1 (MQ=60)
                  TACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCC                                                                                                                                    >  SRR19973066.656440/1‑150 (MQ=60)
                     CATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCT                                                                                                                                 <  SRR19973066.2592794/150‑1 (MQ=60)
                      ATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTC                                                                                                                                >  SRR19973066.1257306/1‑150 (MQ=60)
                        CATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCT                                                                                                                              >  SRR19973066.2846230/1‑150 (MQ=60)
                           CGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCGGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGC                                                                                                                           <  SRR19973066.576576/150‑1 (MQ=60)
                            GGGTTAGCGTTATTTAAGCACCTCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCATCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCACCTCCTGGCA                                                                                                                          <  SRR19973066.2844405/150‑1 (MQ=60)
                                GAGCGTTATTTAAGCACCGCATTGTTTTCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGA                                                                                                                      >  SRR19973066.2752069/1‑150 (MQ=60)
                                GAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGA                                                                                                                      >  SRR19973066.173985/1‑150 (MQ=60)
                                  GCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATT                                                                                                                    <  SRR19973066.691847/150‑1 (MQ=60)
                                   CGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTC                                                                                                                   >  SRR19973066.2384208/1‑150 (MQ=60)
                                    GTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCA                                                                                                                  >  SRR19973066.255961/1‑150 (MQ=60)
                                        TTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCT                                                                                                              <  SRR19973066.1085199/150‑1 (MQ=60)
                                         TTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTG                                                                                                             <  SRR19973066.3390977/150‑1 (MQ=60)
                                         TTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTG                                                                                                             <  SRR19973066.428848/150‑1 (MQ=60)
                                         TTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTG                                                                                                             <  SRR19973066.3390911/150‑1 (MQ=60)
                                         TTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTG                                                                                                             <  SRR19973066.1755976/150‑1 (MQ=60)
                                         TTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTG                                                                                                             <  SRR19973066.1755813/150‑1 (MQ=60)
                                          TAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGT                                                                                                            <  SRR19973066.1862969/150‑1 (MQ=60)
                                              CACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGA                                                                                                        >  SRR19973066.3130200/1‑150 (MQ=60)
                                               ACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAA                                                                                                       >  SRR19973066.671571/1‑150 (MQ=60)
                                                CCTCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAC                                                                                                      <  SRR19973066.2471488/150‑1 (MQ=60)
                                                CCGGATTGTTTGCCGATCTCTTCACTGAGAAATAATTGCAACTGTTATGCCAGCTTGTTAAAAATGCGTAAACAAGGACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAC                                                                                                      <  SRR19973066.2472497/150‑1 (MQ=60)
                                                CCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAACAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAC                                                                                                      <  SRR19973066.2928952/150‑1 (MQ=60)
                                                CCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAC                                                                                                      <  SRR19973066.2934531/150‑1 (MQ=60)
                                                 CGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACT                                                                                                     >  SRR19973066.2386343/1‑150 (MQ=60)
                                                 CGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACT                                                                                                     >  SRR19973066.2386084/1‑150 (MQ=60)
                                                 CGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACT                                                                                                     >  SRR19973066.2810988/1‑150 (MQ=60)
                                                    ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                  <  SRR19973066.800766/150‑1 (MQ=60)
                                                    ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                  <  SRR19973066.2598210/150‑1 (MQ=60)
                                                    ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                  <  SRR19973066.3071836/150‑1 (MQ=60)
                                                    ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                  <  SRR19973066.3075210/150‑1 (MQ=60)
                                                     TTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTA                                                                                                 >  SRR19973066.2344278/1‑150 (MQ=60)
                                                           GCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTTGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAC                                                                                           <  SRR19973066.80751/150‑1 (MQ=60)
                                                            CCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACC                                                                                          <  SRR19973066.2691061/150‑1 (MQ=60)
                                                              GATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAG                                                                                        >  SRR19973066.376999/1‑150 (MQ=60)
                                                                  TCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTA                                                                                    <  SRR19973066.391214/150‑1 (MQ=60)
                                                                       ACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAT                                                                               <  SRR19973066.3543749/150‑1 (MQ=60)
                                                                       ACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAT                                                                               <  SRR19973066.3506869/150‑1 (MQ=60)
                                                                       ACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAT                                                                               <  SRR19973066.1732984/150‑1 (MQ=60)
                                                                             AAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGAT                                                                         <  SRR19973066.1419605/150‑1 (MQ=60)
                                                                             AAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGAT                                                                         >  SRR19973066.222662/1‑150 (MQ=60)
                                                                             AAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGAT                                                                         <  SRR19973066.3204110/150‑1 (MQ=60)
                                                                             AAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGAT                                                                         >  SRR19973066.2632146/1‑150 (MQ=60)
                                                                               AGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGC                                                                       <  SRR19973066.2845820/150‑1 (MQ=60)
                                                                                    TGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATTCGCGAC                                                                  >  SRR19973066.2544813/1‑150 (MQ=60)
                                                                                    TGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAA                                                                  >  SRR19973066.672604/1‑150 (MQ=60)
                                                                                      CAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAAT                                                                <  SRR19973066.1244427/150‑1 (MQ=60)
                                                                                           GTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATC                                                           <  SRR19973066.914542/150‑1 (MQ=60)
                                                                                            TAATTCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCG                                                          >  SRR19973066.2319362/1‑150 (MQ=60)
                                                                                            TAATTCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCG                                                          >  SRR19973066.2319242/1‑150 (MQ=60)
                                                                                            TAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCG                                                          <  SRR19973066.3298918/150‑1 (MQ=60)
                                                                                             AATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGT                                                         >  SRR19973066.1726688/1‑150 (MQ=60)
                                                                                             AATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGT                                                         >  SRR19973066.1726558/1‑150 (MQ=60)
                                                                                              ATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTT                                                        >  SRR19973066.2735609/1‑150 (MQ=60)
                                                                                               TGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTA                                                       <  SRR19973066.1236124/150‑1 (MQ=60)
                                                                                                GCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTAC                                                      <  SRR19973066.751666/150‑1 (MQ=60)
                                                                                                   AGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGG                                                   <  SRR19973066.1625558/150‑1 (MQ=60)
                                                                                                   AGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGG                                                   <  SRR19973066.1625660/150‑1 (MQ=60)
                                                                                                           AAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCA                                           >  SRR19973066.3431208/1‑150 (MQ=60)
                                                                                                               ATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGC                                       >  SRR19973066.2287959/1‑150 (MQ=60)
                                                                                                               ATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGC                                       >  SRR19973066.1464496/1‑150 (MQ=60)
                                                                                                               ATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGC                                       >  SRR19973066.1464206/1‑150 (MQ=60)
                                                                                                               ATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGC                                       >  SRR19973066.2289773/1‑150 (MQ=60)
                                                                                                                TGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCT                                      <  SRR19973066.1649901/150‑1 (MQ=60)
                                                                                                                TGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCT                                      <  SRR19973066.3803/150‑1 (MQ=60)
                                                                                                                        AAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAT                              >  SRR19973066.912168/1‑150 (MQ=60)
                                                                                                                        AAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAT                              >  SRR19973066.1379033/1‑150 (MQ=60)
                                                                                                                        AAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAT                              <  SRR19973066.833508/150‑1 (MQ=60)
                                                                                                                         AAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGANCTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATT                             >  SRR19973066.1822954/1‑150 (MQ=60)
                                                                                                                         AAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGANCTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATT                             >  SRR19973066.1822778/1‑150 (MQ=60)
                                                                                                                         AAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATT                             <  SRR19973066.750842/150‑1 (MQ=60)
                                                                                                                          AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                            >  SRR19973066.3408098/1‑150 (MQ=60)
                                                                                                                          AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                            <  SRR19973066.1540383/150‑1 (MQ=60)
                                                                                                                          AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                            >  SRR19973066.3410665/1‑150 (MQ=60)
                                                                                                                          AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                            <  SRR19973066.3322705/150‑1 (MQ=60)
                                                                                                                           GCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTT                           <  SRR19973066.3223809/150‑1 (MQ=60)
                                                                                                                           GCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTT                           <  SRR19973066.3546220/150‑1 (MQ=60)
                                                                                                                             ACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTAT                         <  SRR19973066.3037190/150‑1 (MQ=60)
                                                                                                                              CCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAGTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATG                        >  SRR19973066.2616212/1‑150 (MQ=60)
                                                                                                                               CTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGA                       >  SRR19973066.3515470/1‑150 (MQ=60)
                                                                                                                               CTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGA                       >  SRR19973066.587512/1‑150 (MQ=60)
                                                                                                                               CTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGA                       >  SRR19973066.587568/1‑150 (MQ=60)
                                                                                                                                TTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTACTCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAT                      <  SRR19973066.1861955/150‑1 (MQ=60)
                                                                                                                                TTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAT                      <  SRR19973066.1861689/150‑1 (MQ=60)
                                                                                                                                TTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGAT                      <  SRR19973066.2091789/150‑1 (MQ=60)
                                                                                                                                    TAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGG                  <  SRR19973066.2398020/150‑1 (MQ=60)
                                                                                                                                    TAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGG                  <  SRR19973066.1706937/150‑1 (MQ=60)
                                                                                                                                     AGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGT                 >  SRR19973066.2250582/1‑150 (MQ=60)
                                                                                                                                     AGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGT                 <  SRR19973066.2823326/150‑1 (MQ=60)
                                                                                                                                     AGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGT                 <  SRR19973066.1281281/150‑1 (MQ=60)
                                                                                                                                     AGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGT                 <  SRR19973066.3142449/150‑1 (MQ=60)
                                                                                                                                      GGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTT                <  SRR19973066.1304014/150‑1 (MQ=60)
                                                                                                                                      GGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTT                <  SRR19973066.1302037/150‑1 (MQ=60)
                                                                                                                                      GGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTT                >  SRR19973066.2855356/1‑150 (MQ=60)
                                                                                                                                       GTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTC               <  SRR19973066.620536/150‑1 (MQ=60)
                                                                                                                                       GTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTC               >  SRR19973066.2507214/1‑150 (MQ=60)
                                                                                                                                       GTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTC               >  SRR19973066.28342/1‑150 (MQ=60)
                                                                                                                                       GTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTC               >  SRR19973066.28348/1‑150 (MQ=60)
                                                                                                                                          CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT            <  SRR19973066.708635/150‑1 (MQ=60)
                                                                                                                                          CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT            >  SRR19973066.1433422/1‑150 (MQ=60)
                                                                                                                                          CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT            >  SRR19973066.598092/1‑150 (MQ=60)
                                                                                                                                          CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT            <  SRR19973066.708622/150‑1 (MQ=60)
                                                                                                                                          CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT            >  SRR19973066.1428954/1‑150 (MQ=60)
                                                                                                                                              TTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTT        <  SRR19973066.2999874/150‑1 (MQ=60)
                                                                                                                                                TGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCC      <  SRR19973066.2243458/150‑1 (MQ=60)
                                                                                                                                                  TGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTT    >  SRR19973066.1791598/1‑150 (MQ=60)
                                                                                                                                                   GGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTT   <  SRR19973066.2324182/150‑1 (MQ=60)
                                                                                                                                                    GTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTA  <  SRR19973066.165145/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTA  >  CP009273/2526645‑2526942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑