Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,526,794 C→T 88.9% intergenic (+55/‑329) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,526,7940CT88.9% 806.6 / 76.2 271intergenic (+55/‑329)cysK/ptsHcysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
Reads supporting (aligned to +/- strand):  ref base C (14/16);  new base T (124/116);  total (138/133)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTA  >  CP009273/2526648‑2526942
                                                                                                                                                  |                                                                                                                                                    
  atTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGc                                                                                                                                                  <  1:444628/149‑1 (MQ=255)
  atTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGc                                                                                                                                                  <  2:794389/149‑1 (MQ=255)
     gtgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTc                                                                                                                                               >  2:1296827/1‑149 (MQ=255)
     gtgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTc                                                                                                                                               >  1:99934/1‑149 (MQ=255)
          tATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACt                                                                                                                                          >  1:1485712/1‑149 (MQ=255)
             tCTACAATCAACGGGTGAGCGTTATATAAGCACCGCATTGTTTGCCGAACTCTTCACTGAGAAAGAATTGAAACAGTAATGCCAGCTTGTTAAAATTGCGTAAAAAAGCACCTTTTTAGGTGCTATTTTGAGGTCTGCTTCAAACttta                                                                                                                                       >  2:1366087/1‑148 (MQ=255)
               tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGc                                                                                                                                     >  2:1500343/1‑149 (MQ=255)
               tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGc                                                                                                                                     >  2:2377631/1‑149 (MQ=255)
               tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGc                                                                                                                                     >  2:1509173/1‑149 (MQ=255)
                aCCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGcc                                                                                                                                    <  2:1682634/149‑1 (MQ=255)
                   atcatcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCcct                                                                                                                                 <  2:523517/149‑1 (MQ=255)
                      atcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcct                                                                                                                              <  2:4787165/149‑1 (MQ=255)
                       tcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTg                                                                                                                             <  1:2044567/149‑1 (MQ=255)
                             gAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGACAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTg                                                                                                                       >  1:2679792/1‑149 (MQ=255)
                              aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGa                                                                                                                      <  2:89692/149‑1 (MQ=255)
                              aGCGTTATTGAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGa                                                                                                                      <  2:3065221/149‑1 (MQ=255)
                                cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCTCTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGAtt                                                                                                                    <  1:4763715/149‑1 (MQ=255)
                                cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGAGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCGGGCATTGAtt                                                                                                                    <  2:4584010/149‑1 (MQ=255)
                                     tttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                                               <  1:4710104/149‑1 (MQ=255)
                                     tttAAGCACCGCAGTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                                               <  2:3032286/149‑1 (MQ=255)
                                     attAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                                               <  1:498885/148‑1 (MQ=255)
                                           cACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCgg                                                                                                         <  2:265755/149‑1 (MQ=255)
                                                 aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                                                                                                   <  1:172262/149‑1 (MQ=255)
                                                 aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                                                                                                   >  2:3797074/1‑149 (MQ=255)
                                                     tttGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCGTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAtt                                                                                               <  1:1297978/149‑1 (MQ=255)
                                                       tGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTa                                                                                             <  2:2797904/149‑1 (MQ=255)
                                                        gCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTaa                                                                                            <  2:959669/149‑1 (MQ=255)
                                                        gCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTaa                                                                                            <  1:4282167/149‑1 (MQ=255)
                                                         ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTAc                                                                                           <  2:3818660/149‑1 (MQ=255)
                                                            aTCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAg                                                                                        >  1:2741067/1‑149 (MQ=255)
                                                            aTCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAg                                                                                        >  1:3292576/1‑149 (MQ=255)
                                                            aTCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTGTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg                                                                                        >  2:3475150/1‑149 (MQ=255)
                                                             tctcTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGa                                                                                       <  1:987833/149‑1 (MQ=255)
                                                              ctctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTACAAACTTACGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGAc                                                                                      <  2:1203515/149‑1 (MQ=255)
                                                               tctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTATCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACt                                                                                     >  2:1963668/1‑149 (MQ=255)
                                                                 ttCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa                                                                                   >  2:677810/1‑149 (MQ=255)
                                                                   cACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGTCTGTCGGAACTGGTATTTAACCAGACTAatt                                                                                 <  2:2941403/149‑1 (MQ=255)
                                                                    aCTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAatta                                                                                <  2:131686/149‑1 (MQ=255)
                                                                     cTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAattat                                                                               >  2:3938128/1‑149 (MQ=255)
                                                                      tGAGAAATAATTGCAACAGTAATGCCAGCTTGTTAAAAATGAGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCTAACTTTAGCCCATCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAataatt                                                                              >  2:3135212/1‑149 (MQ=255)
                                                                      tGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAattatt                                                                              >  2:4701462/1‑149 (MQ=255)
                                                                      tGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAattatt                                                                              <  2:1858357/149‑1 (MQ=255)
                                                                      tGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAattatt                                                                              <  2:784491/149‑1 (MQ=255)
                                                                       gagaAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAttt                                                                             <  2:4448541/149‑1 (MQ=255)
                                                                        agaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAtttt                                                                            >  2:694086/1‑149 (MQ=255)
                                                                        agaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAtttt                                                                            <  1:3718690/149‑1 (MQ=255)
                                                                         gaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTg                                                                           >  1:893246/1‑149 (MQ=255)
                                                                          aaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGa                                                                          >  2:645376/1‑149 (MQ=255)
                                                                          aaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGa                                                                          >  2:645095/1‑149 (MQ=255)
                                                                          aaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGa                                                                          >  2:369536/1‑149 (MQ=255)
                                                                             gaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATgc                                                                       >  2:3665417/1‑149 (MQ=255)
                                                                                   cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                                                 <  1:1037762/149‑1 (MQ=255)
                                                                                   cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                                                 <  2:215406/149‑1 (MQ=255)
                                                                                    aaCAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAAt                                                                <  2:1159098/149‑1 (MQ=255)
                                                                                     aCAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAAtt                                                               >  2:1234357/1‑149 (MQ=255)
                                                                                       aGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTaa                                                             <  1:2631259/149‑1 (MQ=255)
                                                                                        gTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAAt                                                            <  1:1595472/149‑1 (MQ=255)
                                                                                          aaTGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCg                                                          >  2:2055772/1‑149 (MQ=255)
                                                                                            tGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGtt                                                        >  1:3405522/1‑149 (MQ=255)
                                                                                            tGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGtt                                                        <  2:2679784/149‑1 (MQ=255)
                                                                                             gCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATTCGCGAAATTAATCGTTa                                                       >  1:3173398/1‑149 (MQ=255)
                                                                                             gCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTa                                                       >  1:555453/1‑149 (MQ=255)
                                                                                               cAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACa                                                     >  1:1376969/1‑149 (MQ=255)
                                                                                                aGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAGCTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAg                                                    >  1:3645242/1‑149 (MQ=255)
                                                                                                 gCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAgg                                                   >  2:1601436/1‑149 (MQ=255)
                                                                                                 gCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATGTTGATGCGCGAAATTAATCGTTACAgg                                                   >  1:2590767/1‑149 (MQ=255)
                                                                                                   ttGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGaa                                                 >  1:4534307/1‑149 (MQ=255)
                                                                                                      ttAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAg                                              <  2:1090154/149‑1 (MQ=255)
                                                                                                      ttAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAg                                              >  1:2374891/1‑149 (MQ=255)
                                                                                                        aaaaaTGCGTAAAAAAGCACCTTTTTNGGTGCTGTTTTGTNGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGACTGTCGGAACTGGTATTTAACCAGACTAATTATTTAGATGCGNNAAATAAATCGATACAGGAAAAGcc                                            >  1:2798690/1‑149 (MQ=255)
                                                                                                           aaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCaaa                                         >  1:4097382/1‑149 (MQ=255)
                                                                                                           aaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCaaa                                         <  1:3147502/149‑1 (MQ=255)
                                                                                                            aTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAg                                        >  1:4087003/1‑149 (MQ=255)
                                                                                                            aTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAg                                        >  2:1402284/1‑149 (MQ=255)
                                                                                                            aTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAg                                        <  1:1639570/149‑1 (MQ=255)
                                                                                                            aTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAg                                        >  2:2181602/1‑149 (MQ=255)
                                                                                                             tGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                                       <  2:4212596/149‑1 (MQ=255)
                                                                                                             tGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                                       >  1:1490902/1‑149 (MQ=255)
                                                                                                              gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCt                                      <  2:2279234/149‑1 (MQ=255)
                                                                                                                 tAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGaa                                   <  1:1941319/149‑1 (MQ=255)
                                                                                                                  aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt                                  <  1:2717070/149‑1 (MQ=255)
                                                                                                                  aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt                                  <  1:4175685/149‑1 (MQ=255)
                                                                                                                  aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt                                  <  2:4216425/149‑1 (MQ=255)
                                                                                                                   aaaaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATc                                 <  2:3385070/149‑1 (MQ=255)
                                                                                                                     aaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGACAAGCCAAAGCTGACTCGa                               >  2:3263624/1‑149 (MQ=255)
                                                                                                                     aaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa                               <  1:2188005/149‑1 (MQ=255)
                                                                                                                     aaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa                               >  1:1140058/1‑149 (MQ=255)
                                                                                                                     aaaGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa                               >  2:4020434/1‑149 (MQ=255)
                                                                                                                        gCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAttt                            >  1:2723557/1‑149 (MQ=255)
                                                                                                                         cACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtttt                           >  2:1061329/1‑149 (MQ=255)
                                                                                                                          aCCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTa                          >  2:3173181/1‑149 (MQ=255)
                                                                                                                                  aGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTgg                  >  1:3830396/1‑149 (MQ=255)
                                                                                                                                   ggTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGt                 >  1:4337401/1‑149 (MQ=255)
                                                                                                                                          ttttGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTCAATCGATTTTATGATTTGGTTCAAttc          <  2:3578105/149‑1 (MQ=255)
                                                                                                                                            ttGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAttctt        <  1:1213138/149‑1 (MQ=255)
                                                                                                                                              gtgGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAATAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTcc      <  1:645095/149‑1 (MQ=255)
                                                                                                                                              gtgGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTcc      <  1:645376/149‑1 (MQ=255)
                                                                                                                                              gtgGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTcc      <  1:918517/149‑1 (MQ=255)
                                                                                                                                              gtgGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTcc      >  1:3534248/1‑149 (MQ=255)
                                                                                                                                                  ccTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTa  <  2:3292576/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTA  >  CP009273/2526648‑2526942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTT  >  CP009273/2526647‑2526941
                                                                                                                                                   |                                                                                                                                                   
AATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCT                                                                                                                                                   <  SRR19973071.1553764/150‑1 (MQ=60)
   ATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCT                                                                                                                                                <  SRR19973071.446216/150‑1 (MQ=60)
     TGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTC                                                                                                                                              >  SRR19973071.100451/1‑150 (MQ=60)
     TGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTC                                                                                                                                              >  SRR19973071.100386/1‑150 (MQ=60)
        GGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAA                                                                                                                                           >  SRR19973071.1094012/1‑150 (MQ=60)
          TTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACT                                                                                                                                         >  SRR19973071.1491313/1‑150 (MQ=60)
          TTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACT                                                                                                                                         >  SRR19973071.1491190/1‑150 (MQ=60)
          TTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACT                                                                                                                                         >  SRR19973071.1491107/1‑150 (MQ=60)
           TATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTT                                                                                                                                        >  SRR19973071.906583/1‑150 (MQ=60)
                  CCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCC                                                                                                                                 >  SRR19973071.954774/1‑150 (MQ=60)
                     TCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCC                                                                                                                              >  SRR19973071.218437/1‑150 (MQ=60)
                     TCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCC                                                                                                                              >  SRR19973071.4227580/1‑150 (MQ=60)
                       ATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTG                                                                                                                            >  SRR19973071.119389/1‑150 (MQ=60)
                        TCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGG                                                                                                                           <  SRR19973071.2052063/150‑1 (MQ=60)
                           GGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCAT                                                                                                                        >  SRR19973071.632783/1‑150 (MQ=60)
                            GTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATT                                                                                                                       <  SRR19973071.1599517/150‑1 (MQ=60)
                            GTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATT                                                                                                                       <  SRR19973071.4155091/150‑1 (MQ=60)
                             TGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATTACAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCATGGCATTG                                                                                                                      >  SRR19973071.2689377/1‑150 (MQ=60)
                             TGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTG                                                                                                                      >  SRR19973071.2689369/1‑150 (MQ=60)
                               AGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGAT                                                                                                                    <  SRR19973071.858242/150‑1 (MQ=60)
                                 CGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCTCTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTC                                                                                                                  <  SRR19973071.4780545/150‑1 (MQ=60)
                                    TATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGC                                                                                                               <  SRR19973071.2704444/150‑1 (MQ=60)
                                      TTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCT                                                                                                             <  SRR19973071.4726765/150‑1 (MQ=60)
                                      TTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCT                                                                                                             <  SRR19973071.4726812/150‑1 (MQ=60)
                                      ATTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCT                                                                                                             <  SRR19973071.500737/150‑1 (MQ=60)
                                          AGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCG                                                                                                         >  SRR19973071.1467481/1‑150 (MQ=60)
                                           GCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGG                                                                                                        <  SRR19973071.3433544/150‑1 (MQ=60)
                                                GCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTG                                                                                                   <  SRR19973071.456838/150‑1 (MQ=60)
                                                 CATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGG                                                                                                  >  SRR19973071.4539350/1‑150 (MQ=60)
                                                 CATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGG                                                                                                  >  SRR19973071.1826156/1‑150 (MQ=60)
                                                  ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                 <  SRR19973071.172995/150‑1 (MQ=60)
                                                  ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                 >  SRR19973071.620954/1‑150 (MQ=60)
                                                  ATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGT                                                                                                 >  SRR19973071.2575785/1‑150 (MQ=60)
                                                    TGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAT                                                                                               >  SRR19973071.50474/1‑150 (MQ=60)
                                                      TTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCGTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTT                                                                                             <  SRR19973071.1302716/150‑1 (MQ=60)
                                                        TGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAA                                                                                           <  SRR19973071.1521235/150‑1 (MQ=60)
                                                         GCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAC                                                                                          <  SRR19973071.4297328/150‑1 (MQ=60)
                                                         GCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAC                                                                                          <  SRR19973071.4297588/150‑1 (MQ=60)
                                                          CCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTTAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACC                                                                                         <  SRR19973071.2284139/150‑1 (MQ=60)
                                                           CGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCA                                                                                        >  SRR19973071.4091287/1‑150 (MQ=60)
                                                            GATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAG                                                                                       >  SRR19973071.3304244/1‑150 (MQ=60)
                                                            GATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAG                                                                                       >  SRR19973071.3303387/1‑150 (MQ=60)
                                                            GATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAG                                                                                       >  SRR19973071.2750785/1‑150 (MQ=60)
                                                              TCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGAC                                                                                     <  SRR19973071.383437/150‑1 (MQ=60)
                                                              TCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGAC                                                                                     <  SRR19973071.991425/150‑1 (MQ=60)
                                                               CTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACT                                                                                    <  SRR19973071.1288997/150‑1 (MQ=60)
                                                                TCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTA                                                                                   <  SRR19973071.4271397/150‑1 (MQ=60)
                                                                TCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTA                                                                                   <  SRR19973071.2691747/150‑1 (MQ=60)
                                                                   TCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATT                                                                                <  SRR19973071.3401149/150‑1 (MQ=60)
                                                                        GAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTT                                                                           >  SRR19973071.4150452/1‑150 (MQ=60)
                                                                         AGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTG                                                                          <  SRR19973071.3731865/150‑1 (MQ=60)
                                                                         AGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTG                                                                          >  SRR19973071.896479/1‑150 (MQ=60)
                                                                         AGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTG                                                                          <  SRR19973071.3731140/150‑1 (MQ=60)
                                                                            AAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATG                                                                       >  SRR19973071.2261946/1‑150 (MQ=60)
                                                                             AGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGC                                                                      >  SRR19973071.486576/1‑150 (MQ=60)
                                                                                ATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCG                                                                   <  SRR19973071.1711141/150‑1 (MQ=60)
                                                                                    CAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAAT                                                               <  SRR19973071.1041503/150‑1 (MQ=60)
                                                                                     AACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATT                                                              >  SRR19973071.2504143/1‑150 (MQ=60)
                                                                                        AGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAAT                                                           <  SRR19973071.2640992/150‑1 (MQ=60)
                                                                                        AGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAAT                                                           <  SRR19973071.2640737/150‑1 (MQ=60)
                                                                                        AGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAAT                                                           <  SRR19973071.2640707/150‑1 (MQ=60)
                                                                                         GTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATC                                                          <  SRR19973071.4025970/150‑1 (MQ=60)
                                                                                         GTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATC                                                          <  SRR19973071.1601102/150‑1 (MQ=60)
                                                                                            ATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTT                                                       <  SRR19973071.837216/150‑1 (MQ=60)
                                                                                            ATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTT                                                       >  SRR19973071.3417582/1‑150 (MQ=60)
                                                                                            ATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTT                                                       >  SRR19973071.3417583/1‑150 (MQ=60)
                                                                                            ACGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTT                                                       >  SRR19973071.3417561/1‑150 (MQ=60)
                                                                                             TGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATTCGCGAAATTAATCGTTA                                                      >  SRR19973071.3184464/1‑150 (MQ=60)
                                                                                             TGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTA                                                      >  SRR19973071.1643116/1‑150 (MQ=60)
                                                                                             TGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTA                                                      >  SRR19973071.557542/1‑150 (MQ=60)
                                                                                               CCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACA                                                    >  SRR19973071.1647459/1‑150 (MQ=60)
                                                                                               CCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACA                                                    >  SRR19973071.1382049/1‑150 (MQ=60)
                                                                                                CAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAGCTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAG                                                   >  SRR19973071.3658070/1‑150 (MQ=60)
                                                                                                CAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAG                                                   >  SRR19973071.1460105/1‑150 (MQ=60)
                                                                                                CAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAG                                                   >  SRR19973071.985709/1‑150 (MQ=60)
                                                                                                CAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAG                                                   >  SRR19973071.3658562/1‑150 (MQ=60)
                                                                                                 AGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATGTTGATGCGCGAAATTAATCGTTACAGG                                                  >  SRR19973071.2600156/1‑150 (MQ=60)
                                                                                                   CTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAA                                                >  SRR19973071.4550455/1‑150 (MQ=60)
                                                                                                    TTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAA                                               <  SRR19973071.2757131/150‑1 (MQ=60)
                                                                                                      GTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAG                                             >  SRR19973071.2383465/1‑150 (MQ=60)
                                                                                                      GTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAG                                             <  SRR19973071.2873172/150‑1 (MQ=60)
                                                                                                        TAAAAATGCGTAAAAAAGCACCTTTTTNGGTGCTGTTTTGTNGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGACTGTCGGAACTGGTATTTAACCAGACTAATTATTTAGATGCGNNAAATAAATCGATACAGGAAAAGCC                                           >  SRR19973071.2808524/1‑150 (MQ=60)
                                                                                                        TAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCC                                           >  SRR19973071.2808472/1‑150 (MQ=60)
                                                                                                           AAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAA                                        >  SRR19973071.4111872/1‑150 (MQ=60)
                                                                                                           AAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAA                                        >  SRR19973071.4111940/1‑150 (MQ=60)
                                                                                                            AATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAG                                       <  SRR19973071.3158524/150‑1 (MQ=60)
                                                                                                            AATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAG                                       >  SRR19973071.4101517/1‑150 (MQ=60)
                                                                                                             ATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGC                                      >  SRR19973071.1496305/1‑150 (MQ=60)
                                                                                                             ATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGC                                      <  SRR19973071.1645522/150‑1 (MQ=60)
                                                                                                                CGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGA                                   <  SRR19973071.1345282/150‑1 (MQ=60)
                                                                                                                 GTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAA                                  >  SRR19973071.3609861/1‑150 (MQ=60)
                                                                                                                  TAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAT                                 <  SRR19973071.1914596/150‑1 (MQ=60)
                                                                                                                  TAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAT                                 <  SRR19973071.1948389/150‑1 (MQ=60)
                                                                                                                  TAAAAAAGAACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAT                                 <  SRR19973071.4345810/150‑1 (MQ=60)
                                                                                                                   AAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATC                                <  SRR19973071.2726742/150‑1 (MQ=60)
                                                                                                                   AAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATC                                <  SRR19973071.2726751/150‑1 (MQ=60)
                                                                                                                   AAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATC                                <  SRR19973071.4190553/150‑1 (MQ=60)
                                                                                                                    AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCG                               <  SRR19973071.4486287/150‑1 (MQ=60)
                                                                                                                     AAAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGA                              >  SRR19973071.1144039/1‑150 (MQ=60)
                                                                                                                      AAAGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAT                             <  SRR19973071.2196095/150‑1 (MQ=60)
                                                                                                                       AAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATT                            >  SRR19973071.542950/1‑150 (MQ=60)
                                                                                                                        AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                           >  SRR19973071.2733221/1‑150 (MQ=60)
                                                                                                                        AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                           >  SRR19973071.2733243/1‑150 (MQ=60)
                                                                                                                        AGCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTT                           >  SRR19973071.2734404/1‑150 (MQ=60)
                                                                                                                         GCACCTTTTTAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAGTTT                          >  SRR19973071.4439285/1‑150 (MQ=60)
                                                                                                                                  TAGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGG                 >  SRR19973071.3843975/1‑150 (MQ=60)
                                                                                                                                   AGGTGCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGT                >  SRR19973071.4352698/1‑150 (MQ=60)
                                                                                                                                       GCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAA            >  SRR19973071.896879/1‑150 (MQ=60)
                                                                                                                                       GCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAA            <  SRR19973071.2945214/150‑1 (MQ=60)
                                                                                                                                       GCTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAA            <  SRR19973071.1144366/150‑1 (MQ=60)
                                                                                                                                        CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT           <  SRR19973071.1287273/150‑1 (MQ=60)
                                                                                                                                        CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT           >  SRR19973071.3161589/1‑150 (MQ=60)
                                                                                                                                        CTTTTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAAT           >  SRR19973071.3161574/1‑150 (MQ=60)
                                                                                                                                           TTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCT        <  SRR19973071.2024986/150‑1 (MQ=60)
                                                                                                                                           TTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCT        <  SRR19973071.2024310/150‑1 (MQ=60)
                                                                                                                                           TTTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCT        <  SRR19973071.374034/150‑1 (MQ=60)
                                                                                                                                            TTTGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTT       <  SRR19973071.4745010/150‑1 (MQ=60)
                                                                                                                                            TTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTT       <  SRR19973071.4771804/150‑1 (MQ=60)
                                                                                                                                             TTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTTACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTC      <  SRR19973071.1217324/150‑1 (MQ=60)
                                                                                                                                              TGTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCC     >  SRR19973071.3546614/1‑150 (MQ=60)
                                                                                                                                               GTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAATAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCT    <  SRR19973071.647455/150‑1 (MQ=60)
                                                                                                                                               GTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCT    <  SRR19973071.647736/150‑1 (MQ=60)
                                                                                                                                               GTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCT    <  SRR19973071.647702/150‑1 (MQ=60)
                                                                                                                                               GTGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCT    <  SRR19973071.921842/150‑1 (MQ=60)
                                                                                                                                                TGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTT   >  SRR19973071.1865437/1‑150 (MQ=60)
                                                                                                                                                TGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTT   >  SRR19973071.1865468/1‑150 (MQ=60)
                                                                                                                                                TGGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGGTCAATTCTTCCTT   >  SRR19973071.1866011/1‑150 (MQ=60)
                                                                                                                                                 GGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTT  <  SRR19973071.2465399/150‑1 (MQ=60)
                                                                                                                                                 GGTCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTT  <  SRR19973071.2465481/150‑1 (MQ=60)
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AATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTT  >  CP009273/2526647‑2526941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑