Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,597,173 T→G 100% L666R (CTG→CGG)  hyfB → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,597,1730TG97.7% 853.5 / 1.3 214L666R (CTG→CGG) hyfBhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base G (99/110);  total (102/112)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

GGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGC  >  CP009273/2597027‑2597317
                                                                                                                                                  |                                                                                                                                                
ggCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGtgctg                                                                                                                                                 >  2:1197525/1‑148 (MQ=255)
  cTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTgcggct                                                                                                                                               >  2:789492/1‑148 (MQ=255)
     agagCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAt                                                                                                                                            >  1:766067/1‑148 (MQ=255)
         cacaGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCt                                                                                                                                        >  2:2506932/1‑148 (MQ=255)
          acaGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTa                                                                                                                                       <  2:3351340/148‑1 (MQ=255)
          acaGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTa                                                                                                                                       <  1:1782985/148‑1 (MQ=255)
          acaGAACCCTTCTGGGATGAGCGGGCGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTa                                                                                                                                       <  1:2425221/148‑1 (MQ=255)
           caGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAt                                                                                                                                      <  2:799146/148‑1 (MQ=255)
           caGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAt                                                                                                                                      <  1:256707/148‑1 (MQ=255)
           caGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAt                                                                                                                                      <  2:2863554/148‑1 (MQ=255)
             gAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTg                                                                                                                                    <  1:1494846/148‑1 (MQ=255)
              aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGc                                                                                                                                   >  1:2442858/1‑148 (MQ=255)
               aCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTCATCGCTATTGcc                                                                                                                                  >  2:1863156/1‑148 (MQ=255)
                cccTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGACg                                                                                                                                 >  1:2638261/1‑148 (MQ=255)
                 ccTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGt                                                                                                                                <  1:3353653/148‑1 (MQ=255)
                 ccTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGt                                                                                                                                >  1:1290284/1‑148 (MQ=255)
                 ccTTCTGGGAAGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGt                                                                                                                                <  1:1666817/148‑1 (MQ=255)
                           tGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAAt                                                                                                                      <  1:3400688/148‑1 (MQ=255)
                            gAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATc                                                                                                                     <  2:184812/148‑1 (MQ=255)
                             aGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCa                                                                                                                    >  1:908481/1‑148 (MQ=255)
                                   tGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAACTCACCATga                                                                                                              >  2:1712108/1‑148 (MQ=255)
                                   tGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATga                                                                                                              <  1:2777736/148‑1 (MQ=255)
                                   tGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATga                                                                                                              >  2:3476142/1‑148 (MQ=255)
                                     aTCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATgaga                                                                                                            <  1:145777/148‑1 (MQ=255)
                                      tCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGAc                                                                                                           <  2:1594621/148‑1 (MQ=255)
                                      tCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGAc                                                                                                           >  1:1947520/1‑148 (MQ=255)
                                        cGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaa                                                                                                         <  1:995695/148‑1 (MQ=255)
                                         gCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaaa                                                                                                        <  2:2336350/148‑1 (MQ=255)
                                         gCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaaa                                                                                                        <  2:1891273/148‑1 (MQ=255)
                                          ccccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAAc                                                                                                       >  1:707949/1‑148 (MQ=255)
                                            ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActc                                                                                                     >  2:1980279/1‑148 (MQ=255)
                                            ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActc                                                                                                     >  2:910918/1‑148 (MQ=255)
                                             cATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActct                                                                                                    <  2:2734073/148‑1 (MQ=255)
                                             cATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActct                                                                                                    >  1:554901/1‑148 (MQ=255)
                                              aTCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCtt                                                                                                   <  1:2201687/148‑1 (MQ=255)
                                              aTCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCtt                                                                                                   <  2:2413658/148‑1 (MQ=255)
                                                cGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTg                                                                                                 >  1:1995606/1‑148 (MQ=255)
                                                  tGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCg                                                                                               <  2:3427622/148‑1 (MQ=255)
                                                   gAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGa                                                                                              >  2:2664317/1‑148 (MQ=255)
                                                    aGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAc                                                                                             <  1:327936/148‑1 (MQ=255)
                                                    aGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAc                                                                                             <  1:2878126/148‑1 (MQ=255)
                                                     gcgcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACg                                                                                            >  2:408880/1‑148 (MQ=255)
                                                     gcgcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACg                                                                                            >  2:3185087/1‑148 (MQ=255)
                                                       gcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGa                                                                                          <  1:1724869/148‑1 (MQ=255)
                                                        ccACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGat                                                                                         >  1:567622/1‑148 (MQ=255)
                                                           cccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATc                                                                                      <  2:503666/148‑1 (MQ=255)
                                                            ccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCt                                                                                     <  1:677767/148‑1 (MQ=255)
                                                               aCGGCTGGCCAAAGACATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGt                                                                                  <  2:2201578/148‑1 (MQ=255)
                                                               aCGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGt                                                                                  <  2:1816522/148‑1 (MQ=255)
                                                                cGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTc                                                                                 >  2:869398/1‑148 (MQ=255)
                                                                cGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTc                                                                                 >  2:1090391/1‑148 (MQ=255)
                                                                  gCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAt                                                                               <  1:93217/148‑1 (MQ=255)
                                                                   cTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAtt                                                                              <  1:408420/148‑1 (MQ=255)
                                                                   cTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAtt                                                                              <  1:3549142/148‑1 (MQ=255)
                                                                     ggCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGACTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAtttt                                                                            <  2:2106169/148‑1 (MQ=255)
                                                                      gCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAttttt                                                                           <  2:1468146/148‑1 (MQ=255)
                                                                           aGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt                                                                      >  1:1060233/1‑148 (MQ=255)
                                                                           aGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt                                                                      >  1:882726/1‑148 (MQ=255)
                                                                              aaTACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGc                                                                   <  2:3148704/148‑1 (MQ=255)
                                                                                tACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGcac                                                                 >  2:662471/1‑148 (MQ=255)
                                                                                    gCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGc                                                             <  1:112495/148‑1 (MQ=255)
                                                                                    gCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGc                                                             <  1:2104592/148‑1 (MQ=255)
                                                                                     cATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGcc                                                            >  2:2790783/1‑148 (MQ=255)
                                                                                      aTCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCg                                                           <  1:2418706/148‑1 (MQ=255)
                                                                                          gCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt                                                       <  2:139098/148‑1 (MQ=255)
                                                                                           cAAAGCGGCGACTTTCGTCTCTATTGCCTGTAAGTGGTAGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGaat                                                      >  2:2954277/1‑146 (MQ=255)
                                                                                            aaaGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTc                                                     <  2:2306089/148‑1 (MQ=255)
                                                                                              aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctg                                                   >  1:3260916/1‑148 (MQ=255)
                                                                                               gcggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgc                                                  <  2:2529074/148‑1 (MQ=255)
                                                                                               gcggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgc                                                  <  2:2047515/148‑1 (MQ=255)
                                                                                               gcggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgc                                                  >  1:3464461/1‑148 (MQ=255)
                                                                                                  gcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGa                                               <  2:3066941/148‑1 (MQ=255)
                                                                                                    gACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATg                                             <  1:2506932/148‑1 (MQ=255)
                                                                                                     aCTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGc                                            <  2:832935/148‑1 (MQ=255)
                                                                                                        ttCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTaa                                         >  1:3524519/1‑148 (MQ=255)
                                                                                                        ttCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATAGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATATGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTaa                                         >  1:1339698/1‑148 (MQ=255)
                                                                                                            tctcTATTGCCTGTATGTGGTCGCCTCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcccc                                     >  1:1269777/1‑148 (MQ=255)
                                                                                                            tctcTATTGCCTGTATGTGGTCGCCTCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcccc                                     >  1:3048758/1‑148 (MQ=255)
                                                                                                             ctctATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCa                                    <  2:1947520/148‑1 (MQ=255)
                                                                                                                 aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttt                                >  1:2722624/1‑148 (MQ=255)
                                                                                                                 aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttt                                <  1:2786004/148‑1 (MQ=255)
                                                                                                                 aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttt                                <  1:2854770/148‑1 (MQ=255)
                                                                                                                 aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCGATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAGTCTGCTGATGCTAACCCCACttt                                >  1:3103780/1‑148 (MQ=255)
                                                                                                                  ttGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACtttt                               <  2:3518103/148‑1 (MQ=255)
                                                                                                                   tGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttttt                              >  2:1354898/1‑148 (MQ=255)
                                                                                                                   tGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttttt                              >  2:3129027/1‑148 (MQ=255)
                                                                                                                    gCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTa                             <  1:2653198/148‑1 (MQ=255)
                                                                                                                     ccTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTAc                            >  2:2252802/1‑148 (MQ=255)
                                                                                                                      cTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAACGCTATTGCCGTCTAAGGAAATCAACATGATACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTATTCTGATGCTAACCCCACATTTTACg                           >  1:1596368/1‑148 (MQ=255)
                                                                                                                        gTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACggg                         <  2:1507442/148‑1 (MQ=255)
                                                                                                                          aTGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTa                       >  1:2204289/1‑148 (MQ=255)
                                                                                                                           tgtgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAt                      <  1:1516839/148‑1 (MQ=255)
                                                                                                                             tgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTCttt                    >  2:1643632/1‑148 (MQ=255)
                                                                                                                             tgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAttt                    <  1:1532856/148‑1 (MQ=255)
                                                                                                                                tCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTccc                 >  1:186625/1‑148 (MQ=255)
                                                                                                                                  gccgcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCgg               <  2:1246329/148‑1 (MQ=255)
                                                                                                                                       aCTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt          <  2:707949/148‑1 (MQ=255)
                                                                                                                                       aCTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt          <  1:1020456/148‑1 (MQ=255)
                                                                                                                                            ttGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATGTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTCTTTCCCGGCAGATAcgcg     >  2:2160925/1‑148 (MQ=255)
                                                                                                                                               tgcggcTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgcgc  >  2:329413/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGC  >  CP009273/2597027‑2597317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGC  >  CP009273/2597024‑2597317
                                                                                                                                                     |                                                                                                                                                
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                  <  SRR19973066.3199526/150‑1 (MQ=60)
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                  <  SRR19973066.3027990/150‑1 (MQ=60)
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                  >  SRR19973066.1812202/1‑150 (MQ=60)
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                  >  SRR19973066.1255268/1‑150 (MQ=60)
 AGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATCGCCTGTATGTGGTCGCCGCACTGGTTGTGCGG                                                                                                                                                 >  SRR19973066.1932943/1‑150 (MQ=60)
      TCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAT                                                                                                                                            >  SRR19973066.771741/1‑150 (MQ=60)
        AGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCG                                                                                                                                          <  SRR19973066.784361/150‑1 (MQ=60)
            CACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAT                                                                                                                                      <  SRR19973066.1625284/150‑1 (MQ=60)
             ACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATT                                                                                                                                     <  SRR19973066.1795033/150‑1 (MQ=60)
             ACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATT                                                                                                                                     <  SRR19973066.2441590/150‑1 (MQ=60)
             ACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATT                                                                                                                                     <  SRR19973066.1795716/150‑1 (MQ=60)
             ACAGAACCCTTCTGGGATGAGCGGGCGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATT                                                                                                                                     <  SRR19973066.2441693/150‑1 (MQ=60)
              CAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTG                                                                                                                                    <  SRR19973066.258653/150‑1 (MQ=60)
               AGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGC                                                                                                                                   >  SRR19973066.2459500/1‑150 (MQ=60)
                GAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCC                                                                                                                                  <  SRR19973066.1505285/150‑1 (MQ=60)
                GAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCC                                                                                                                                  >  SRR19973066.3354159/1‑150 (MQ=60)
                 AACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGACG                                                                                                                                 >  SRR19973066.2656016/1‑150 (MQ=60)
                  ACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGT                                                                                                                                >  SRR19973066.1299621/1‑150 (MQ=60)
                  ACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGT                                                                                                                                >  SRR19973066.1299620/1‑150 (MQ=60)
                    CCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCT                                                                                                                              <  SRR19973066.904889/150‑1 (MQ=60)
                    CCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCT                                                                                                                              <  SRR19973066.1523030/150‑1 (MQ=60)
                    CCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCT                                                                                                                              <  SRR19973066.3375998/150‑1 (MQ=60)
                    CCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCT                                                                                                                              <  SRR19973066.1980910/150‑1 (MQ=60)
                    CCTTCTGGGAAGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCT                                                                                                                              <  SRR19973066.1678963/150‑1 (MQ=60)
                       TCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAG                                                                                                                           <  SRR19973066.2019483/150‑1 (MQ=60)
                        CTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGG                                                                                                                          <  SRR19973066.1100607/150‑1 (MQ=60)
                          GGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAA                                                                                                                        >  SRR19973066.3420018/1‑150 (MQ=60)
                           GGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAA                                                                                                                       >  SRR19973066.1538953/1‑150 (MQ=60)
                             ATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATC                                                                                                                     <  SRR19973066.3568617/150‑1 (MQ=60)
                             ATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATC                                                                                                                     >  SRR19973066.3260906/1‑150 (MQ=60)
                              TGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCA                                                                                                                    <  SRR19973066.3423392/150‑1 (MQ=60)
                              TGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCA                                                                                                                    >  SRR19973066.914911/1‑150 (MQ=60)
                                      TGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGA                                                                                                            <  SRR19973066.2796438/150‑1 (MQ=60)
                                      TGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGA                                                                                                            <  SRR19973066.2796385/150‑1 (MQ=60)
                                      TGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGA                                                                                                            <  SRR19973066.1661710/150‑1 (MQ=60)
                                       GATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGAC                                                                                                           >  SRR19973066.1961316/1‑150 (MQ=60)
                                        ATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACA                                                                                                          <  SRR19973066.147160/150‑1 (MQ=60)
                                        ATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACA                                                                                                          <  SRR19973066.147161/150‑1 (MQ=60)
                                           CGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAAC                                                                                                       >  SRR19973066.713188/1‑150 (MQ=60)
                                           CGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAAC                                                                                                       <  SRR19973066.1003032/150‑1 (MQ=60)
                                            GCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACT                                                                                                      <  SRR19973066.718481/150‑1 (MQ=60)
                                              CCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCT                                                                                                    >  SRR19973066.558880/1‑150 (MQ=60)
                                               CCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTT                                                                                                   <  SRR19973066.2556704/150‑1 (MQ=60)
                                                 ATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTG                                                                                                 <  SRR19973066.2216953/150‑1 (MQ=60)
                                                 ATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTG                                                                                                 <  SRR19973066.2216980/150‑1 (MQ=60)
                                                 ATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTG                                                                                                 >  SRR19973066.2009645/1‑150 (MQ=60)
                                                  TCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGC                                                                                                <  SRR19973066.560095/150‑1 (MQ=60)
                                                  TCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGC                                                                                                <  SRR19973066.635450/150‑1 (MQ=60)
                                                   CGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCG                                                                                               >  SRR19973066.1867489/1‑150 (MQ=60)
                                                       AGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGG                                                                                           <  SRR19973066.330433/150‑1 (MQ=60)
                                                       AGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGG                                                                                           <  SRR19973066.2897409/150‑1 (MQ=60)
                                                       AGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGG                                                                                           <  SRR19973066.2896999/150‑1 (MQ=60)
                                                         CGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGAT                                                                                         >  SRR19973066.571642/1‑150 (MQ=60)
                                                          GCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATA                                                                                        <  SRR19973066.1737348/150‑1 (MQ=60)
                                                            CACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATC                                                                                      >  SRR19973066.1988973/1‑150 (MQ=60)
                                                               CCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGG                                                                                   <  SRR19973066.682728/150‑1 (MQ=60)
                                                               CCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGG                                                                                   >  SRR19973066.967644/1‑150 (MQ=60)
                                                                     GCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTT                                                                             <  SRR19973066.94223/150‑1 (MQ=60)
                                                                      CTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTT                                                                            <  SRR19973066.3572700/150‑1 (MQ=60)
                                                                      CTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTT                                                                            <  SRR19973066.411631/150‑1 (MQ=60)
                                                                      CTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTT                                                                            <  SRR19973066.3572743/150‑1 (MQ=60)
                                                                           CAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGAGATCTGGTCATTTTTGCGT                                                                       >  SRR19973066.2642449/1‑150 (MQ=60)
                                                                            AAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTT                                                                      >  SRR19973066.1067558/1‑150 (MQ=60)
                                                                            AAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTT                                                                      >  SRR19973066.1068016/1‑150 (MQ=60)
                                                                            AAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTT                                                                      >  SRR19973066.889000/1‑150 (MQ=60)
                                                                               GAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGAGTTAGC                                                                   >  SRR19973066.2811092/1‑150 (MQ=60)
                                                                                AAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCA                                                                  >  SRR19973066.1733312/1‑150 (MQ=60)
                                                                                  ATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACA                                                                >  SRR19973066.3032918/1‑150 (MQ=60)
                                                                                     CAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGC                                                             >  SRR19973066.428256/1‑150 (MQ=60)
                                                                                      AGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCC                                                            <  SRR19973066.2449422/150‑1 (MQ=60)
                                                                                       GCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCG                                                           <  SRR19973066.1929638/150‑1 (MQ=60)
                                                                                       GCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCG                                                           <  SRR19973066.113640/150‑1 (MQ=60)
                                                                                       GCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCG                                                           <  SRR19973066.2119092/150‑1 (MQ=60)
                                                                                         ATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTTCCGTCTAAGGAAATCACCCTGAGACAAACTCTTTTCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTG                                                         >  SRR19973066.3408201/1‑150 (MQ=60)
                                                                                         ATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTG                                                         <  SRR19973066.2435111/150‑1 (MQ=60)
                                                                                         ATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTG                                                         <  SRR19973066.2431652/150‑1 (MQ=60)
                                                                                            TGCAAAGCGGCGACTTTCGTCTCTCTTGCCTGTATGTGGTCGCCGCACGGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATT                                                      <  SRR19973066.3016107/150‑1 (MQ=60)
                                                                                              ctggagttcagacgtgtgctcttccgatctTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCT                                                    <  SRR19973066.1403896/120‑1 (MQ=60)
                                                                                               AAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTG                                                   >  SRR19973066.3282363/1‑150 (MQ=60)
                                                                                                AAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGC                                                  >  SRR19973066.2459503/1‑150 (MQ=60)
                                                                                                AAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGC                                                  >  SRR19973066.3487063/1‑150 (MQ=60)
                                                                                                AAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGC                                                  >  SRR19973066.3487618/1‑150 (MQ=60)
                                                                                                     GCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCGTGAAACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATG                                             <  SRR19973066.2684049/150‑1 (MQ=60)
                                                                                                      CGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGC                                            >  SRR19973066.3069320/1‑150 (MQ=60)
                                                                                                       GACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCT                                           <  SRR19973066.2524005/150‑1 (MQ=60)
                                                                                                         CTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAA                                         >  SRR19973066.1348440/1‑150 (MQ=60)
                                                                                                         CTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAA                                         >  SRR19973066.3547978/1‑150 (MQ=60)
                                                                                                         CTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATAGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATATGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAA                                         >  SRR19973066.1349422/1‑150 (MQ=60)
                                                                                                             CGTCTCTATTGCCTGTATGTGGTCGCCTCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCC                                     >  SRR19973066.3069140/1‑150 (MQ=60)
                                                                                                             CGTCTCTATTGCCTGTATGTGGTCGCCTCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCC                                     >  SRR19973066.1278947/1‑150 (MQ=60)
                                                                                                                CTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACT                                  >  SRR19973066.559675/1‑150 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                <  SRR19973066.1209668/150‑1 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                >  SRR19973066.2740940/1‑150 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                <  SRR19973066.3536058/150‑1 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCGATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAGTCTGCTGATGCTAACCCCACTTT                                >  SRR19973066.3124510/1‑150 (MQ=60)
                                                                                                                    ATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTT                              <  SRR19973066.2804760/150‑1 (MQ=60)
                                                                                                                    ATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTT                              <  SRR19973066.2873951/150‑1 (MQ=60)
                                                                                                                       GCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACG                           <  SRR19973066.2671006/150‑1 (MQ=60)
                                                                                                                       GCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACG                           <  SRR19973066.2671001/150‑1 (MQ=60)
                                                                                                                       GCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAACGCTATTGCCGTCTAAGGAAATCAACATGATACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTATTCTGATGCTAACCCCACATTTTACG                           >  SRR19973066.1607930/1‑150 (MQ=60)
                                                                                                                        CCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAATTACGTCTAAGGAAAACACCAATAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTTCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTATTACGG                          >  SRR19973066.2454880/1‑150 (MQ=60)
                                                                                                                           GTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTA                       >  SRR19973066.2657157/1‑150 (MQ=60)
                                                                                                                           GTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTA                       >  SRR19973066.2219567/1‑150 (MQ=60)
                                                                                                                             ATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATT                     <  SRR19973066.2210787/150‑1 (MQ=60)
                                                                                                                             ATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTTCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTAAGGGTATT                     >  SRR19973066.1553214/1‑150 (MQ=60)
                                                                                                                              TGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTT                    <  SRR19973066.1527499/150‑1 (MQ=60)
                                                                                                                                TGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCC                  <  SRR19973066.1543767/150‑1 (MQ=60)
                                                                                                                                 GGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCC                 <  SRR19973066.694871/150‑1 (MQ=60)
                                                                                                                                 GGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCC                 >  SRR19973066.188245/1‑150 (MQ=60)
                                                                                                                                 GGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCC                 <  SRR19973066.280276/150‑1 (MQ=60)
                                                                                                                                         CACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCCAACCCCACTTTTTACGGGTATTTCCCGGCAGATA         >  SRR19973066.3289864/1‑150 (MQ=60)
                                                                                                                                          ACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAC        >  SRR19973066.911093/1‑150 (MQ=60)
                                                                                                                                          ACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAC        <  SRR19973066.1027963/150‑1 (MQ=60)
                                                                                                                                           CTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACG       <  SRR19973066.936755/150‑1 (MQ=60)
                                                                                                                                                TGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGC  >  SRR19973066.1495689/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGC  >  CP009273/2597024‑2597317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑