Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,597,173 T→G 100% L666R (CTG→CGG)  hyfB → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,597,1730TG98.6% 876.4 / ‑2.2 216L666R (CTG→CGG) hyfBhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (114/99);  total (116/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

CAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGC  >  CP009273/2597031‑2597315
                                                                                                                                              |                                                                                                                                              
cAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTaa                                                                                                                                           >  1:567524/1‑148 (MQ=255)
 agagCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAt                                                                                                                                          <  1:2423618/148‑1 (MQ=255)
 agagCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAt                                                                                                                                          >  1:1985401/1‑148 (MQ=255)
  gagCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATc                                                                                                                                         >  2:621581/1‑148 (MQ=255)
   agCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTCTGTGGTCGCCGCACTGGTTGTGCGGCTAATCg                                                                                                                                        >  2:721051/1‑148 (MQ=255)
         gAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTg                                                                                                                                  >  2:1616086/1‑148 (MQ=255)
          aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGc                                                                                                                                 >  2:809498/1‑148 (MQ=255)
          aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGc                                                                                                                                 >  1:1556901/1‑148 (MQ=255)
           aCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGcc                                                                                                                                >  1:3208424/1‑148 (MQ=255)
           aCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGcc                                                                                                                                >  2:2684698/1‑148 (MQ=255)
           aCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGcc                                                                                                                                >  1:2307074/1‑148 (MQ=255)
            cccTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCg                                                                                                                               >  1:1893023/1‑148 (MQ=255)
             ccTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGt                                                                                                                              >  2:1185367/1‑148 (MQ=255)
               ttCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCt                                                                                                                            >  2:3239189/1‑148 (MQ=255)
                tCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTa                                                                                                                           >  2:3408304/1‑148 (MQ=255)
                 cTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTaa                                                                                                                          <  1:352717/148‑1 (MQ=255)
                 cTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTaa                                                                                                                          <  1:2808683/148‑1 (MQ=255)
                   gggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAgg                                                                                                                        >  1:1471080/1‑148 (MQ=255)
                    ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGa                                                                                                                       <  1:2029899/148‑1 (MQ=255)
                    ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGa                                                                                                                       >  1:3265302/1‑148 (MQ=255)
                    ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGa                                                                                                                       <  1:3108491/148‑1 (MQ=255)
                    ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGa                                                                                                                       >  2:2491802/1‑148 (MQ=255)
                    ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGa                                                                                                                       <  1:1804126/148‑1 (MQ=255)
                       tGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAAt                                                                                                                    <  2:1744205/148‑1 (MQ=255)
                       tGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAAt                                                                                                                    <  2:1128619/148‑1 (MQ=255)
                       tGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAAt                                                                                                                    <  1:1764028/148‑1 (MQ=255)
                        gAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATc                                                                                                                   <  2:1308898/148‑1 (MQ=255)
                           cGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCAcc                                                                                                                <  1:717938/148‑1 (MQ=255)
                               tGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATga                                                                                                            >  2:177735/1‑148 (MQ=255)
                                    cGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaa                                                                                                       <  2:1763503/148‑1 (MQ=255)
                                    cGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaa                                                                                                       <  2:2628123/148‑1 (MQ=255)
                                    cGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaa                                                                                                       >  2:3106794/1‑148 (MQ=255)
                                     gCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaaa                                                                                                      <  2:477536/148‑1 (MQ=255)
                                      ccccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAAc                                                                                                     <  1:2063595/148‑1 (MQ=255)
                                       cccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAAct                                                                                                    <  1:1819482/148‑1 (MQ=255)
                                        ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActc                                                                                                   >  1:214636/1‑148 (MQ=255)
                                        ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActc                                                                                                   <  2:801360/148‑1 (MQ=255)
                                        ccATCGTGAGCGCCACAAAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGTCTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActc                                                                                                   <  2:2773036/148‑1 (MQ=255)
                                         cATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActct                                                                                                  >  1:3585634/1‑148 (MQ=255)
                                           tCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCttt                                                                                                >  1:192670/1‑148 (MQ=255)
                                           tCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCttt                                                                                                >  2:3630730/1‑148 (MQ=255)
                                            cGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTg                                                                                               >  1:3183490/1‑148 (MQ=255)
                                              tGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCg                                                                                             <  1:1312907/148‑1 (MQ=255)
                                               gAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGa                                                                                            <  2:2474350/148‑1 (MQ=255)
                                               gAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGa                                                                                            <  2:986352/148‑1 (MQ=255)
                                                  cgcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACgg                                                                                         >  1:1481346/1‑148 (MQ=255)
                                                       cccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATc                                                                                    >  2:1683492/1‑148 (MQ=255)
                                                        ccAACGGCTGGCCAACGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCt                                                                                   <  1:1538190/148‑1 (MQ=255)
                                                         cAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTg                                                                                  >  1:2996058/1‑148 (MQ=255)
                                                          aaCGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCCTGAGACAAACTCTTTGCGACGGATATCTgg                                                                                 >  2:3466897/1‑148 (MQ=255)
                                                          aaCGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTgg                                                                                 >  2:2899442/1‑148 (MQ=255)
                                                          aaCGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTgg                                                                                 >  1:2699153/1‑148 (MQ=255)
                                                           aCGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGt                                                                                >  1:3679447/1‑148 (MQ=255)
                                                           aCGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCAAGATACAAACTCTTTTAGACGGATATCTgtt                                                                                >  1:3033397/1‑146 (MQ=255)
                                                            cGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTc                                                                               >  1:309401/1‑148 (MQ=255)
                                                                     aaaGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTCTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCg                                                                      <  2:3652/148‑1 (MQ=255)
                                                                      aaGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGt                                                                     >  2:578697/1‑148 (MQ=255)
                                                                       aGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt                                                                    >  1:2548571/1‑148 (MQ=255)
                                                                       aGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt                                                                    >  1:2491815/1‑148 (MQ=255)
                                                                       aGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt                                                                    >  1:2205889/1‑148 (MQ=255)
                                                                        gAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTa                                                                   >  2:1201379/1‑148 (MQ=255)
                                                                            tACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGcac                                                               >  2:1338396/1‑148 (MQ=255)
                                                                            tACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGcac                                                               <  2:2264169/148‑1 (MQ=255)
                                                                             aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGcaca                                                              >  2:373417/1‑148 (MQ=255)
                                                                               aGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAgg                                                            <  2:1685564/148‑1 (MQ=255)
                                                                               aGCATCTGCAAAGCGGCGACTTTCGTCTCTAGTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAgg                                                            <  2:497463/148‑1 (MQ=255)
                                                                                gCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGc                                                           >  1:1065909/1‑148 (MQ=255)
                                                                                gCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGc                                                           >  1:3619417/1‑148 (MQ=255)
                                                                                 cATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGcc                                                          <  2:3326384/148‑1 (MQ=255)
                                                                                  aTCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCg                                                         <  1:2869956/148‑1 (MQ=255)
                                                                                      gCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt                                                     >  2:413983/1‑148 (MQ=255)
                                                                                       cAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt                                                    <  1:2650264/148‑1 (MQ=255)
                                                                                       cAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt                                                    <  1:2371722/148‑1 (MQ=255)
                                                                                       cAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt                                                    >  1:1530973/1‑148 (MQ=255)
                                                                                        aaaGCGGCGACTTTCGCCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTc                                                   <  1:1096576/148‑1 (MQ=255)
                                                                                          aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctg                                                 <  2:2198069/148‑1 (MQ=255)
                                                                                          aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctg                                                 >  2:751061/1‑148 (MQ=255)
                                                                                          aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctg                                                 >  2:1658669/1‑148 (MQ=255)
                                                                                          aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATAACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctg                                                 >  1:1022395/1‑148 (MQ=255)
                                                                                              gcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGa                                             >  1:1141620/1‑148 (MQ=255)
                                                                                                     tCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAc                                      >  1:2885893/1‑148 (MQ=255)
                                                                                                      cGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcc                                     >  1:3128993/1‑148 (MQ=255)
                                                                                                         ctctATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCa                                  >  2:3180285/1‑148 (MQ=255)
                                                                                                          tctATTGCCTGTATGTGGTCGCAGCACTGGTTGTGCGGCTATTAGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAACCCAc                                 <  2:1591253/148‑1 (MQ=255)
                                                                                                           ctATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACt                                <  1:3761894/148‑1 (MQ=255)
                                                                                                                  cTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACg                         >  2:606357/1‑148 (MQ=255)
                                                                                                                  cTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACg                         <  2:2753844/148‑1 (MQ=255)
                                                                                                                   tGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACgg                        >  2:1004118/1‑148 (MQ=255)
                                                                                                                     tATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGt                      <  1:474023/148‑1 (MQ=255)
                                                                                                                     tATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGt                      <  2:567524/148‑1 (MQ=255)
                                                                                                                      aTGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTATTTACGGGTa                     >  2:2662345/1‑148 (MQ=255)
                                                                                                                         tgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAttt                  >  2:3034125/1‑148 (MQ=255)
                                                                                                                         tgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTCATGCTAACCCCACTAATTACGGGTAtat                  >  2:734623/1‑146 (MQ=255)
                                                                                                                         tgGTCGCCGCACTGGTTGTGCGACTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAttt                  <  2:1092965/148‑1 (MQ=255)
                                                                                                                           gTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTcc                >  2:1889685/1‑148 (MQ=255)
                                                                                                                                cgcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCa           >  2:1519077/1‑148 (MQ=255)
                                                                                                                                 gcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAg          <  1:3630765/148‑1 (MQ=255)
                                                                                                                                        ttGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcg   >  2:1989130/1‑148 (MQ=255)
                                                                                                                                         tgtgCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgc  >  1:452801/1‑148 (MQ=255)
                                                                                                                                         tgtgCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgc  >  1:775893/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGC  >  CP009273/2597031‑2597315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTAT  >  CP009273/2597024‑2597321
                                                                                                                                                     |                                                                                                                                                    
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCGGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGTGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                      <  SRR19973069.2858321/150‑1 (MQ=60)
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                      >  SRR19973069.3483980/1‑150 (MQ=60)
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                      <  SRR19973069.2858395/150‑1 (MQ=60)
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCG                                                                                                                                                      >  SRR19973069.3480451/1‑150 (MQ=60)
   GGCTCTGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCT                                                                                                                                                   <  SRR19973069.2524593/150‑1 (MQ=60)
   GGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCT                                                                                                                                                   <  SRR19973069.596060/150‑1 (MQ=60)
   GGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCT                                                                                                                                                   <  SRR19973069.2542987/150‑1 (MQ=60)
   GGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCT                                                                                                                                                   <  SRR19973069.3403623/150‑1 (MQ=60)
   GGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCT                                                                                                                                                   <  SRR19973069.2524625/150‑1 (MQ=60)
     CTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAA                                                                                                                                                 >  SRR19973069.571465/1‑150 (MQ=60)
      TCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAT                                                                                                                                                >  SRR19973069.1999223/1‑150 (MQ=60)
       CAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATC                                                                                                                                               <  SRR19973069.2974053/150‑1 (MQ=60)
        AGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCG                                                                                                                                              <  SRR19973069.2439979/150‑1 (MQ=60)
        AGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCG                                                                                                                                              <  SRR19973069.506689/150‑1 (MQ=60)
        AGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCG                                                                                                                                              <  SRR19973069.2069673/150‑1 (MQ=60)
               AGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGC                                                                                                                                       >  SRR19973069.45696/1‑150 (MQ=60)
               AGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGC                                                                                                                                       >  SRR19973069.1567537/1‑150 (MQ=60)
                GAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCC                                                                                                                                      >  SRR19973069.2322774/1‑150 (MQ=60)
                GAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCC                                                                                                                                      >  SRR19973069.3229332/1‑150 (MQ=60)
                GAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCC                                                                                                                                      >  SRR19973069.3229728/1‑150 (MQ=60)
                GAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCC                                                                                                                                      <  SRR19973069.1497330/150‑1 (MQ=60)
                 AACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCG                                                                                                                                     >  SRR19973069.1906142/1‑150 (MQ=60)
                        CTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGG                                                                                                                              <  SRR19973069.2827301/150‑1 (MQ=60)
                        CTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGG                                                                                                                              <  SRR19973069.355233/150‑1 (MQ=60)
                        CTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGG                                                                                                                              >  SRR19973069.1481374/1‑150 (MQ=60)
                        CTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGG                                                                                                                              >  SRR19973069.1481334/1‑150 (MQ=60)
                        CTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGG                                                                                                                              <  SRR19973069.2292193/150‑1 (MQ=60)
                         TGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGA                                                                                                                             >  SRR19973069.3286691/1‑150 (MQ=60)
                           GGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAA                                                                                                                           <  SRR19973069.3128938/150‑1 (MQ=60)
                           GGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAA                                                                                                                           <  SRR19973069.3128799/150‑1 (MQ=60)
                           GGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAA                                                                                                                           <  SRR19973069.1816818/150‑1 (MQ=60)
                           GGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAA                                                                                                                           <  SRR19973069.2044001/150‑1 (MQ=60)
                              TGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCA                                                                                                                        <  SRR19973069.1776466/150‑1 (MQ=60)
                               GAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCAC                                                                                                                       <  SRR19973069.2147473/150‑1 (MQ=60)
                                  CGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCAT                                                                                                                    <  SRR19973069.722997/150‑1 (MQ=60)
                                    GGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGA                                                                                                                  >  SRR19973069.1519708/1‑150 (MQ=60)
                                       GATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGAC                                                                                                               >  SRR19973069.169302/1‑150 (MQ=60)
                                            GCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACT                                                                                                          <  SRR19973069.3386331/150‑1 (MQ=60)
                                             CCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTC                                                                                                         <  SRR19973069.2077903/150‑1 (MQ=60)
                                             CCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTC                                                                                                         >  SRR19973069.216321/1‑150 (MQ=60)
                                              CCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCT                                                                                                        <  SRR19973069.1832516/150‑1 (MQ=60)
                                              CCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCT                                                                                                        <  SRR19973069.1832308/150‑1 (MQ=60)
                                              CCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCT                                                                                                        <  SRR19973069.1832255/150‑1 (MQ=60)
                                              CCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCT                                                                                                        <  SRR19973069.2448139/150‑1 (MQ=60)
                                              CCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCT                                                                                                        >  SRR19973069.3609021/1‑150 (MQ=60)
                                                CATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTT                                                                                                      >  SRR19973069.194237/1‑150 (MQ=60)
                                                CATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTT                                                                                                      >  SRR19973069.1792022/1‑150 (MQ=60)
                                                 ATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTG                                                                                                     <  SRR19973069.2354231/150‑1 (MQ=60)
                                                 ATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTG                                                                                                     >  SRR19973069.3204187/1‑150 (MQ=60)
                                                    GTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGA                                                                                                  >  SRR19973069.2398929/1‑150 (MQ=60)
                                                    GTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGA                                                                                                  >  SRR19973069.462597/1‑150 (MQ=60)
                                                     TGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAC                                                                                                 <  SRR19973069.1322621/150‑1 (MQ=60)
                                                     TGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAC                                                                                                 <  SRR19973069.1321894/150‑1 (MQ=60)
                                                       AGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGG                                                                                               >  SRR19973069.1491649/1‑150 (MQ=60)
                                                             ACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCT                                                                                         <  SRR19973069.2041592/150‑1 (MQ=60)
                                                              CCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTG                                                                                        >  SRR19973069.3015945/1‑150 (MQ=60)
                                                               CCAACGGCTGGCCAACGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGG                                                                                       <  SRR19973069.1548737/150‑1 (MQ=60)
                                                               CCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGG                                                                                       >  SRR19973069.2717240/1‑150 (MQ=60)
                                                               CCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGG                                                                                       <  SRR19973069.1549095/150‑1 (MQ=60)
                                                                CAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGT                                                                                      >  SRR19973069.3703512/1‑150 (MQ=60)
                                                                CAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGT                                                                                      >  SRR19973069.3081501/1‑150 (MQ=60)
                                                                CAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCAAGATACAAACTCTTTTAGACGGATATCTGTT                                                                                      >  SRR19973069.3053509/1‑150 (MQ=60)
                                                                 AACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTC                                                                                     >  SRR19973069.311475/1‑150 (MQ=60)
                                                                     GCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTT                                                                                 <  SRR19973069.3102804/150‑1 (MQ=60)
                                                                       TGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTT                                                                               <  SRR19973069.2205156/150‑1 (MQ=60)
                                                                       TGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTT                                                                               <  SRR19973069.802459/150‑1 (MQ=60)
                                                                           CAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGT                                                                           >  SRR19973069.2539310/1‑150 (MQ=60)
                                                                            AAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTT                                                                          >  SRR19973069.2565416/1‑150 (MQ=60)
                                                                            AAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTT                                                                          >  SRR19973069.2508483/1‑150 (MQ=60)
                                                                            AAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTT                                                                          >  SRR19973069.2220849/1‑150 (MQ=60)
                                                                                  ATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCCTTTTTGCGTTAGCACA                                                                    >  SRR19973069.3694925/1‑150 (MQ=60)
                                                                                  ATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACA                                                                    >  SRR19973069.1821658/1‑150 (MQ=60)
                                                                                     CAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGC                                                                 >  SRR19973069.3643063/1‑150 (MQ=60)
                                                                                     CAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGC                                                                 >  SRR19973069.1072904/1‑150 (MQ=60)
                                                                                     CAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGC                                                                 >  SRR19973069.1073333/1‑150 (MQ=60)
                                                                                         ATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTG                                                             <  SRR19973069.2888807/150‑1 (MQ=60)
                                                                                         ATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTG                                                             >  SRR19973069.2858329/1‑150 (MQ=60)
                                                                                         ATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTG                                                             >  SRR19973069.3767585/1‑150 (MQ=60)
                                                                                           CTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACGGGCCGTGAT                                                           <  SRR19973069.1913132/150‑1 (MQ=60)
                                                                                            TGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATT                                                          >  SRR19973069.1541495/1‑150 (MQ=60)
                                                                                              CAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCT                                                        <  SRR19973069.2387817/150‑1 (MQ=60)
                                                                                              CAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCT                                                        <  SRR19973069.2668017/150‑1 (MQ=60)
                                                                                              CAAAGCGGCGACTTTCGTATCTATTGCCTGTATGTGGTCGCCGCACTTGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCT                                                        <  SRR19973069.2668005/150‑1 (MQ=60)
                                                                                               AAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTG                                                       <  SRR19973069.2642802/150‑1 (MQ=60)
                                                                                               AAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATAACCATGAGACAAACTCTTTGAGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTG                                                       >  SRR19973069.1029467/1‑150 (MQ=60)
                                                                                               AAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACAGGTTGTGCGGCTAATCGCTATTTCCGTCTAAGGAAATCACCATGAGACAAACTCGTTGCGACGGATAACTGGTCATTTTTTCGTTAGCACAGGCCGTGATTCTG                                                       >  SRR19973069.1545745/1‑150 (MQ=60)
                                                                                               AAAGCGGCGACTTTCGCCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTG                                                       <  SRR19973069.1104240/150‑1 (MQ=60)
                                                                                               AAAGCGGCGACTTTCGCCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTG                                                       <  SRR19973069.1104202/150‑1 (MQ=60)
                                                                                                 AGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCT                                                     >  SRR19973069.3338206/1‑150 (MQ=60)
                                                                                                   CGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGA                                                   >  SRR19973069.1149586/1‑150 (MQ=60)
                                                                                                    GGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGAT                                                  <  SRR19973069.1136559/150‑1 (MQ=60)
                                                                                                    GGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGAT                                                  <  SRR19973069.2210316/150‑1 (MQ=60)
                                                                                                          TTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTTCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAC                                            >  SRR19973069.2904881/1‑150 (MQ=60)
                                                                                                          TTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAC                                            >  SRR19973069.2904889/1‑150 (MQ=60)
                                                                                                           TTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACC                                           >  SRR19973069.3149519/1‑150 (MQ=60)
                                                                                                             CGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCC                                         >  SRR19973069.1960063/1‑150 (MQ=60)
                                                                                                              GTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCA                                        >  SRR19973069.3138765/1‑150 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                    <  SRR19973069.765179/150‑1 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                    >  SRR19973069.3591554/1‑150 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                    <  SRR19973069.3228204/150‑1 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTT                                    <  SRR19973069.3786349/150‑1 (MQ=60)
                                                                                                                  CTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAATGCCGTCTAAGGAAATAACCAAGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGTTGCTAACCCCACTTT                                    >  SRR19973069.1193331/1‑150 (MQ=60)
                                                                                                                            TATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAT                          >  SRR19973069.3023375/1‑150 (MQ=60)
                                                                                                                            TATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAT                          <  SRR19973069.477474/150‑1 (MQ=60)
                                                                                                                                  GTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAATCTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCG                    <  SRR19973069.625715/150‑1 (MQ=60)
                                                                                                                                        GCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAATGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAT              <  SRR19973069.3654463/150‑1 (MQ=60)
                                                                                                                                        GCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAT              >  SRR19973069.3166707/1‑150 (MQ=60)
                                                                                                                                        GCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAT              <  SRR19973069.3654498/150‑1 (MQ=60)
                                                                                                                                           CTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACG           <  SRR19973069.2702690/150‑1 (MQ=60)
                                                                                                                                            TGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACTGGTATTTCCCGGCAGATACGC          >  SRR19973069.363169/1‑150 (MQ=60)
                                                                                                                                             GGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCG         >  SRR19973069.2821460/1‑150 (MQ=60)
                                                                                                                                              GTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGC        >  SRR19973069.456112/1‑150 (MQ=60)
                                                                                                                                              GTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGC        >  SRR19973069.456090/1‑150 (MQ=60)
                                                                                                                                              GTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGC        >  SRR19973069.781394/1‑150 (MQ=60)
                                                                                                                                                    CGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTAT  >  SRR19973069.1422879/1‑150 (MQ=60)
                                                                                                                                                    CGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTAT  >  SRR19973069.1723757/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTAT  >  CP009273/2597024‑2597321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑