Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,597,173	T→G	43.6%	L666R (CTG→CGG)	hyfB →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,597,173	0	T	G	43.6%	51.8 / inf	264	L666R (CTG→CGG)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand): ref base T (79/70); new base G (56/59); total (135/129)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 279 total aligned reads displayed.

CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATG  >  CP009273/2597024‑2597322

cAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGtgct                                                                                                                                                       <  2:383484/150‑1 (MQ=255)

 aGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGtgctg                                                                                                                                                      >  2:985051/1‑150 (MQ=255)

 aGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGtgctg                                                                                                                                                      >  1:3741528/1‑150 (MQ=255)

  gggCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGACGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGtgcggc                                                                                                                                                     <  2:2623120/150‑1 (MQ=255)

       cAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATc                                                                                                                                                >  1:4342176/1‑150 (MQ=255)

       cAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATc                                                                                                                                                <  1:2385670/150‑1 (MQ=255)

       cAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATc                                                                                                                                                <  2:1630456/150‑1 (MQ=255)

          agCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCt                                                                                                                                             <  2:4265356/150‑1 (MQ=255)

               aGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGc                                                                                                                                        <  2:4041897/150‑1 (MQ=255)

                gAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGcc                                                                                                                                       <  2:3442927/150‑1 (MQ=255)

                 aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCg                                                                                                                                      >  1:821358/1‑150 (MQ=255)

                 aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCg                                                                                                                                      <  2:2964003/150‑1 (MQ=255)

                       tCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAg                                                                                                                                >  1:3092158/1‑150 (MQ=255)

                       tCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAg                                                                                                                                <  1:1314359/150‑1 (MQ=255)

                        cTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAgg                                                                                                                               >  2:3541099/1‑150 (MQ=255)

                         tGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTCTTGCCGTCTAAGGa                                                                                                                              >  2:398471/1‑150 (MQ=255)

                           ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGaaa                                                                                                                            >  1:1502284/1‑150 (MQ=255)

                           ggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGaaa                                                                                                                            <  1:1269415/150‑1 (MQ=255)

                              tGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCa                                                                                                                         <  2:2785585/150‑1 (MQ=255)

                                   gggTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGTATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATg                                                                                                                    >  1:3115454/1‑150 (MQ=255)

                                   gggTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATg                                                                                                                    <  1:1759831/150‑1 (MQ=255)

                                       gATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGAc                                                                                                                <  2:2404075/150‑1 (MQ=255)

                                        aTCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACa                                                                                                               >  2:2073611/1‑150 (MQ=255)

                                        aTCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACa                                                                                                               >  2:1296128/1‑150 (MQ=255)

                                           cGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAAc                                                                                                            <  1:4181959/150‑1 (MQ=255)

                                                   cGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATAACCATGAGACAAACTCTTTGCg                                                                                                    <  2:1417152/150‑1 (MQ=255)

                                                    gTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGa                                                                                                   >  2:1837708/1‑150 (MQ=255)

                                                     tGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAc                                                                                                  <  1:170338/150‑1 (MQ=255)

                                                     tGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAc                                                                                                  <  2:3118205/150‑1 (MQ=255)

                                                     tGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAc                                                                                                  >  2:297329/1‑150 (MQ=255)

                                                      gAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACg                                                                                                 <  1:744984/150‑1 (MQ=255)

                                                           ccACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGatat                                                                                            <  2:4335124/150‑1 (MQ=255)

                                                           ccACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGatat                                                                                            <  2:2184732/150‑1 (MQ=255)

                                                              cccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTg                                                                                         <  1:1017957/150‑1 (MQ=255)

                                                              cccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTg                                                                                         >  1:1900840/1‑150 (MQ=255)

                                                              cccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTg                                                                                         >  2:2527351/1‑150 (MQ=255)

                                                               ccAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTgg                                                                                        <  1:960767/150‑1 (MQ=255)

                                                                   cGGCTGGCCAAAGAAAGACAGCCTCGGCAAAGCGGCGACTTGCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGGGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAt                                                                                    <  2:3327932/150‑1 (MQ=255)

                                                                    ggCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGTAAAACACCATGAGACAAACTCTGTCCGACGGATATCTGGTCAtt                                                                                   >  2:2380344/1‑150 (MQ=255)

                                                                     gCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAttt                                                                                  <  1:2212771/150‑1 (MQ=255)

                                                                     gCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAttt                                                                                  <  2:1697414/150‑1 (MQ=255)

                                                                     gCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAttt                                                                                  <  2:669817/150‑1 (MQ=255)

                                                                         gCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGc                                                                              <  1:2320326/150‑1 (MQ=255)

                                                                          ccAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCg                                                                             >  1:2439510/1‑150 (MQ=255)

                                                                            aaaGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt                                                                           >  1:1161415/1‑150 (MQ=255)

                                                                              aGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAg                                                                         >  2:2205714/1‑150 (MQ=255)

                                                                               gAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGc                                                                        >  2:730665/1‑150 (MQ=255)

                                                                               gAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAGTTTTGCGTTAGc                                                                        >  2:2861742/1‑150 (MQ=255)

                                                                                aaaTACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTTCGTTAGca                                                                       >  1:1841444/1‑150 (MQ=255)

                                                                                   tACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAg                                                                    >  1:1090635/1‑150 (MQ=255)

                                                                                   tACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAg                                                                    <  2:148891/150‑1 (MQ=255)

                                                                                    aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAgg                                                                   >  1:439193/1‑150 (MQ=255)

                                                                                    aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAgg                                                                   <  2:229642/150‑1 (MQ=255)

                                                                                    aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAGTTTTGCGTTAGCACAgg                                                                   >  2:3199140/1‑150 (MQ=255)

                                                                                         aTCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTg                                                              >  2:2903026/1‑150 (MQ=255)

                                                                                            tGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt                                                           >  1:4216847/1‑150 (MQ=255)

                                                                                            tGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt                                                           >  1:2761407/1‑150 (MQ=255)

                                                                                              cAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTct                                                         <  1:1320194/150‑1 (MQ=255)

                                                                                               aaaGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctg                                                        <  2:1878216/150‑1 (MQ=255)

                                                                                               aaaGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGATAGCACAGGCCGTGATTctg                                                        >  2:1191635/1‑150 (MQ=255)

                                                                                                aaGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgc                                                       >  1:3192931/1‑150 (MQ=255)

                                                                                                 aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgct                                                      <  2:3704077/150‑1 (MQ=255)

                                                                                                  gcggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgctg                                                     <  2:3493723/150‑1 (MQ=255)

                                                                                                   cggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGa                                                    >  2:506740/1‑150 (MQ=255)

                                                                                                    ggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGAt                                                   >  1:75597/1‑150 (MQ=255)

                                                                                                    ggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGAt                                                   <  1:556011/150‑1 (MQ=255)

                                                                                                      cgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGc                                                 >  1:2701124/1‑150 (MQ=255)

                                                                                                       gACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCt                                                >  2:3425737/1‑150 (MQ=255)

                                                                                                       gACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCt                                                <  2:1750251/150‑1 (MQ=255)

                                                                                                          tttCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAc                                             >  1:2638872/1‑150 (MQ=255)

                                                                                                           ttCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcc                                            <  2:3721250/150‑1 (MQ=255)

                                                                                                           ttCGTCTCTATGGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcc                                            <  2:761492/150‑1 (MQ=255)

                                                                                                              gTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCa                                         <  2:3792723/150‑1 (MQ=255)

                                                                                                               tctcTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCAc                                        <  1:3477734/150‑1 (MQ=255)

                                                                                                                ctctATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACt                                       <  2:4277017/150‑1 (MQ=255)

                                                                                                                    aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttttt                                   <  2:3643302/150‑1 (MQ=255)

                                                                                                                    aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttttt                                   <  2:1041256/150‑1 (MQ=255)

                                                                                                                     ttGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTTCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACATTTTa                                  >  1:2923514/1‑150 (MQ=255)

                                                                                                                       gCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACg                                >  2:4154312/1‑150 (MQ=255)

                                                                                                                         cTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACggg                              >  1:2505490/1‑150 (MQ=255)

                                                                                                                              tgtgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAttt                         <  2:2077348/150‑1 (MQ=255)

                                                                                                                                tgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTcc                       >  1:450322/1‑150 (MQ=255)

                                                                                                                                 ggTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTccc                      >  2:2678800/1‑150 (MQ=255)

                                                                                                                                 ggTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTccc                      <  2:266392/150‑1 (MQ=255)

                                                                                                                                 ggTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTccc                      >  2:787796/1‑150 (MQ=255)

                                                                                                                                  gTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCg                     <  1:1673391/150‑1 (MQ=255)

                                                                                                                                     gccgcACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCa                  >  1:3008593/1‑150 (MQ=255)

                                                                                                                                      ccgcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAg                 >  1:1569677/1‑150 (MQ=255)

                                                                                                                                        gcACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt               <  1:3125499/150‑1 (MQ=255)

                                                                                                                                        gcACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt               <  1:940329/150‑1 (MQ=255)

                                                                                                                                          aCTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAc             <  2:4147989/150‑1 (MQ=255)

                                                                                                                                           cTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcg            >  1:3427096/1‑150 (MQ=255)

                                                                                                                                             ggTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcg          >  1:2071994/1‑150 (MQ=255)

                                                                                                                                                tgtgCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgcgc       >  1:2655347/1‑150 (MQ=255)

                                                                                                                                                 gtgCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgcgcg      >  1:3599381/1‑150 (MQ=255)

                                                                                                                                                  tgctgcTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAGTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGt     >  2:601587/1‑150 (MQ=255)

                                                                                                                                                   gctgctAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTa    >  2:3920223/1‑150 (MQ=255)

                                                                                                                                                    ctgctAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTAt   <  1:3322213/150‑1 (MQ=255)

                                                                                                                                                     tgctAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATg  >  1:2918062/1‑150 (MQ=255)

                                                                                                                                                     tgctAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATg  <  1:4017635/150‑1 (MQ=255)

CAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATG  >  CP009273/2597024‑2597322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A