Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,086,574 T→A 49.3% F93L (TTT→TTA)  yqgE → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,086,5740TA49.3% 10.1 / inf 215F93L (TTT→TTA) yqgEuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (60/49);  new base A (62/44);  total (122/93)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT  >  CP009273/3086429‑3086720
                                                                                                                                                 |                                                                                                                                                  
aTCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGc                                                                                                                                                  >  1:3560224/1‑148 (MQ=255)
 tctcAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCt                                                                                                                                                 >  2:2416077/1‑148 (MQ=255)
   tcAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTc                                                                                                                                               <  1:993567/148‑1 (MQ=255)
   tcAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTc                                                                                                                                               <  1:2442937/148‑1 (MQ=255)
     aaaaTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCa                                                                                                                                             >  1:2945105/1‑148 (MQ=255)
      aaaTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAg                                                                                                                                            >  2:3098861/1‑148 (MQ=255)
        aTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCa                                                                                                                                          <  1:2347044/148‑1 (MQ=255)
          tGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCAtt                                                                                                                                        <  2:3045130/148‑1 (MQ=255)
            aaGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCg                                                                                                                                      >  2:1223664/1‑148 (MQ=255)
             aGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGc                                                                                                                                     >  1:1231646/1‑148 (MQ=255)
              gggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCa                                                                                                                                    <  2:2992639/148‑1 (MQ=255)
              gggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCa                                                                                                                                    >  2:1259825/1‑148 (MQ=255)
                  ttCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTc                                                                                                                                <  1:3268138/148‑1 (MQ=255)
                  ttCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTc                                                                                                                                <  1:475315/148‑1 (MQ=255)
                      ggAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGac                                                                                                                            >  1:1560188/1‑148 (MQ=255)
                       gAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGaca                                                                                                                           >  2:702962/1‑148 (MQ=255)
                       gAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAAACCGACTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGaca                                                                                                                           >  2:283183/1‑148 (MQ=255)
                        aaaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaa                                                                                                                          >  2:1979787/1‑148 (MQ=255)
                          aaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaaca                                                                                                                        <  2:2285979/148‑1 (MQ=255)
                             cTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACgg                                                                                                                     >  1:2046137/1‑148 (MQ=255)
                               gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTa                                                                                                                   <  2:2673835/148‑1 (MQ=255)
                               gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTa                                                                                                                   <  2:3557972/148‑1 (MQ=255)
                                aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTaa                                                                                                                  <  2:1149338/148‑1 (MQ=255)
                                aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTaa                                                                                                                  <  1:2236990/148‑1 (MQ=255)
                                aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTaa                                                                                                                  <  2:2604500/148‑1 (MQ=255)
                                 aGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAAt                                                                                                                 >  1:2853634/1‑148 (MQ=255)
                                  gATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATg                                                                                                                >  1:34931/1‑148 (MQ=255)
                                    tCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGAc                                                                                                              >  2:537456/1‑148 (MQ=255)
                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                       <  2:929708/148‑1 (MQ=255)
                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                       <  1:1210328/148‑1 (MQ=255)
                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                       <  2:3380310/148‑1 (MQ=255)
                                             aGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcg                                                                                                     >  2:1557789/1‑148 (MQ=255)
                                               ccGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcgcg                                                                                                   >  2:1790848/1‑148 (MQ=255)
                                                cgcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGa                                                                                                  <  2:862574/148‑1 (MQ=255)
                                                cgcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGa                                                                                                  <  2:2581632/148‑1 (MQ=255)
                                                  cgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtg                                                                                                >  1:1472356/1‑148 (MQ=255)
                                                    tgatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtgtg                                                                                              <  1:225644/148‑1 (MQ=255)
                                                           tcaatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGaaa                                                                                       >  2:3497079/1‑148 (MQ=255)
                                                            caatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                      >  2:2221698/1‑148 (MQ=255)
                                                            caatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                      >  2:3521016/1‑148 (MQ=255)
                                                            caatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                      >  2:2728397/1‑148 (MQ=255)
                                                             aatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACg                                                                                     <  2:638921/148‑1 (MQ=255)
                                                              atcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGc                                                                                    >  2:3186093/1‑148 (MQ=255)
                                                              atcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGc                                                                                    >  1:2161774/1‑148 (MQ=255)
                                                               tcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCt                                                                                   <  2:203236/148‑1 (MQ=255)
                                                                   tCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGc                                                                               >  1:3430120/1‑148 (MQ=255)
                                                                    cTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAGTTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                              >  1:2102922/1‑148 (MQ=255)
                                                                    cTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                              >  2:290149/1‑148 (MQ=255)
                                                                    cTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                              >  1:1857409/1‑148 (MQ=255)
                                                                        aTAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGa                                                                          >  1:1902805/1‑148 (MQ=255)
                                                                        aTAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGa                                                                          <  1:1742263/148‑1 (MQ=255)
                                                                           aaCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATaa                                                                       >  2:40196/1‑148 (MQ=255)
                                                                               ggTGATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaacaa                                                                   <  2:1101242/148‑1 (MQ=255)
                                                                                  tATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCg                                                                >  2:2226221/1‑148 (MQ=255)
                                                                                   aTGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGt                                                               <  2:2046137/148‑1 (MQ=255)
                                                                                    tGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTc                                                              <  2:1716525/148‑1 (MQ=255)
                                                                                     gCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCt                                                             >  1:2431378/1‑148 (MQ=255)
                                                                                     gCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCt                                                             >  1:641174/1‑148 (MQ=255)
                                                                                          gcggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGt                                                        <  1:2786903/148‑1 (MQ=255)
                                                                                           cggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTa                                                       >  1:2239150/1‑148 (MQ=255)
                                                                                            ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAt                                                      <  1:1548508/148‑1 (MQ=255)
                                                                                            ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAt                                                      <  2:2161774/148‑1 (MQ=255)
                                                                                               ccGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtgg                                                   <  1:1009809/148‑1 (MQ=255)
                                                                                               ccGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtgg                                                   >  1:3243025/1‑148 (MQ=255)
                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                <  1:1407569/148‑1 (MQ=255)
                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                >  2:1674860/1‑148 (MQ=255)
                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                >  2:3433818/1‑148 (MQ=255)
                                                                                                  ctggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggtgg                                                >  2:2463690/1‑148 (MQ=255)
                                                                                                   tggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGc                                               <  2:3384437/148‑1 (MQ=255)
                                                                                                     gctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctc                                             >  1:341931/1‑148 (MQ=255)
                                                                                                     gctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctc                                             >  1:1689326/1‑148 (MQ=255)
                                                                                                     gctgAAGATCGCGGGCTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctc                                             >  1:1240889/1‑148 (MQ=255)
                                                                                                       tgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTg                                           >  2:2880857/1‑148 (MQ=255)
                                                                                                        gAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                          >  1:3122956/1‑148 (MQ=255)
                                                                                                        gAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                          <  2:2061639/148‑1 (MQ=255)
                                                                                                         aaGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTggg                                         >  1:3003457/1‑148 (MQ=255)
                                                                                                         aaGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTggg                                         >  2:3211095/1‑148 (MQ=255)
                                                                                                          aGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGt                                        <  1:3079122/148‑1 (MQ=255)
                                                                                                              cgcgGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATg                                    >  1:3176640/1‑148 (MQ=255)
                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                <  2:2953521/148‑1 (MQ=255)
                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                <  2:2982223/148‑1 (MQ=255)
                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                >  2:816372/1‑148 (MQ=255)
                                                                                                                   gTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcc                               <  1:2774602/148‑1 (MQ=255)
                                                                                                                   gTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcc                               <  1:2675669/148‑1 (MQ=255)
                                                                                                                   gTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcc                               >  1:3389328/1‑148 (MQ=255)
                                                                                                                    tttATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                              >  2:93857/1‑148 (MQ=255)
                                                                                                                    tttATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                              >  2:2658299/1‑148 (MQ=255)
                                                                                                                    tttATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                              <  1:3004405/148‑1 (MQ=255)
                                                                                                                       aTTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTggg                           >  1:886713/1‑148 (MQ=255)
                                                                                                                           tGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGaa                       <  1:2966033/148‑1 (MQ=255)
                                                                                                                              aTACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAgg                    <  1:2555447/148‑1 (MQ=255)
                                                                                                                               tACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGt                   <  1:3060514/148‑1 (MQ=255)
                                                                                                                                   ccGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               <  1:2280539/148‑1 (MQ=255)
                                                                                                                                   ccGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               <  1:1368121/148‑1 (MQ=255)
                                                                                                                                   ccGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGGAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               <  1:535270/148‑1 (MQ=255)
                                                                                                                                   ccGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc               >  1:1888003/1‑148 (MQ=255)
                                                                                                                                           cAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGGCTCCTGGGAGAAAGGTCAACTGGAACaa       <  1:747805/148‑1 (MQ=255)
                                                                                                                                            aaTTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAg      <  2:3210651/148‑1 (MQ=255)
                                                                                                                                              ttttGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGaa    <  1:1110585/148‑1 (MQ=255)
                                                                                                                                              tttaGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGaa    <  2:1611556/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT  >  CP009273/3086429‑3086720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTCT  >  CP009273/3086427‑3086723
                                                                                                                                                   |                                                                                                                                                     
AAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGC                                                                                                                                                     >  SRR19973066.3583834/1‑150 (MQ=60)
AAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGC                                                                                                                                                     <  SRR19973066.2904674/150‑1 (MQ=60)
 AATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCT                                                                                                                                                    >  SRR19973066.2023398/1‑150 (MQ=60)
 AATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCT                                                                                                                                                    <  SRR19973066.1308006/150‑1 (MQ=60)
  ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTT                                                                                                                                                   <  SRR19973066.3506172/150‑1 (MQ=60)
     TCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCA                                                                                                                                                >  SRR19973066.2964661/1‑150 (MQ=60)
     TCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCA                                                                                                                                                <  SRR19973066.1000886/150‑1 (MQ=60)
     TCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCA                                                                                                                                                <  SRR19973066.1000841/150‑1 (MQ=60)
     TCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCA                                                                                                                                                <  SRR19973066.2459580/150‑1 (MQ=60)
       AAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGC                                                                                                                                              >  SRR19973066.560728/1‑150 (MQ=60)
        AAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCA                                                                                                                                             >  SRR19973066.2526906/1‑150 (MQ=60)
          ATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATT                                                                                                                                           <  SRR19973066.2363007/150‑1 (MQ=60)
          ATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATT                                                                                                                                           <  SRR19973066.2362913/150‑1 (MQ=60)
            TGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCG                                                                                                                                         <  SRR19973066.736468/150‑1 (MQ=60)
             GAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGC                                                                                                                                        >  SRR19973066.1109238/1‑150 (MQ=60)
             GAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGC                                                                                                                                        >  SRR19973066.1240400/1‑150 (MQ=60)
                 GGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTT                                                                                                                                    >  SRR19973066.2898347/1‑150 (MQ=60)
                   ATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCA                                                                                                                                  >  SRR19973066.2703076/1‑150 (MQ=60)
                    TTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAG                                                                                                                                 <  SRR19973066.3289604/150‑1 (MQ=60)
                    TTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAG                                                                                                                                 <  SRR19973066.478871/150‑1 (MQ=60)
                    TTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAG                                                                                                                                 <  SRR19973066.478905/150‑1 (MQ=60)
                      CTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGAC                                                                                                                               >  SRR19973066.1571414/1‑150 (MQ=60)
                       TGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACA                                                                                                                              >  SRR19973066.3446085/1‑150 (MQ=60)
                       TGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACA                                                                                                                              >  SRR19973066.3530585/1‑150 (MQ=60)
                          AAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACA                                                                                                                           >  SRR19973066.1728970/1‑150 (MQ=60)
                             AGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGG                                                                                                                        >  SRR19973066.2060370/1‑150 (MQ=60)
                                TGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAA                                                                                                                     >  SRR19973066.443090/1‑150 (MQ=60)
                                 GAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAAT                                                                                                                    >  SRR19973066.2872891/1‑150 (MQ=60)
                                 GAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAAT                                                                                                                    >  SRR19973066.2872811/1‑150 (MQ=60)
                                  AAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATG                                                                                                                   <  SRR19973066.2252419/150‑1 (MQ=60)
                                  AAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATG                                                                                                                   >  SRR19973066.35408/1‑150 (MQ=60)
                                  AAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATG                                                                                                                   <  SRR19973066.2252469/150‑1 (MQ=60)
                                       CACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCAC                                                                                                              >  SRR19973066.1494172/1‑150 (MQ=60)
                                         CGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTT                                                                                                            <  SRR19973066.849419/150‑1 (MQ=60)
                                            CGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCC                                                                                                         >  SRR19973066.2075929/1‑150 (MQ=60)
                                             GGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCG                                                                                                        >  SRR19973066.1442970/1‑150 (MQ=60)
                                             GGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCG                                                                                                        <  SRR19973066.1218798/150‑1 (MQ=60)
                                                  CGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATG                                                                                                   >  SRR19973066.1482733/1‑150 (MQ=60)
                                                      TGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCT                                                                                               <  SRR19973066.227460/150‑1 (MQ=60)
                                                      TGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCT                                                                                               <  SRR19973066.227438/150‑1 (MQ=60)
                                                      TGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCT                                                                                               <  SRR19973066.227504/150‑1 (MQ=60)
                                                           AATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAA                                                                                          >  SRR19973066.308786/1‑150 (MQ=60)
                                                              CAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGC                                                                                       >  SRR19973066.2176794/1‑150 (MQ=60)
                                                              CAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTACCCGCGATGTGATGGAAACGC                                                                                       >  SRR19973066.2176802/1‑150 (MQ=60)
                                                                   CGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGC                                                                                  >  SRR19973066.3453062/1‑150 (MQ=60)
                                                                    GTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAGTTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCA                                                                                 >  SRR19973066.2117403/1‑150 (MQ=60)
                                                                    GTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCA                                                                                 >  SRR19973066.53514/1‑150 (MQ=60)
                                                                    GTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCA                                                                                 >  SRR19973066.1870694/1‑150 (MQ=60)
                                                                        GGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGA                                                                             >  SRR19973066.1916372/1‑150 (MQ=60)
                                                                          ATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATA                                                                           <  SRR19973066.1754818/150‑1 (MQ=60)
                                                                             AACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAAC                                                                        <  SRR19973066.2384460/150‑1 (MQ=60)
                                                                                     ATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCT                                                                <  SRR19973066.2477676/150‑1 (MQ=60)
                                                                                     ATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCT                                                                >  SRR19973066.645809/1‑150 (MQ=60)
                                                                                     ATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCT                                                                >  SRR19973066.2447927/1‑150 (MQ=60)
                                                                                     ATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCT                                                                <  SRR19973066.3119560/150‑1 (MQ=60)
                                                                                      TGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTG                                                               >  SRR19973066.1245341/1‑150 (MQ=60)
                                                                                       GCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGA                                                              <  SRR19973066.1398189/150‑1 (MQ=60)
                                                                                        CTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGAC                                                             >  SRR19973066.3265049/1‑150 (MQ=60)
                                                                                           GGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTA                                                          >  SRR19973066.2254591/1‑150 (MQ=60)
                                                                                            GCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAT                                                         <  SRR19973066.3006342/150‑1 (MQ=60)
                                                                                            GCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAT                                                         <  SRR19973066.2805666/150‑1 (MQ=60)
                                                                                              GGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTG                                                       <  SRR19973066.1559598/150‑1 (MQ=60)
                                                                                               GTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGG                                                      >  SRR19973066.3264416/1‑150 (MQ=60)
                                                                                                 CCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTG                                                    <  SRR19973066.1017248/150‑1 (MQ=60)
                                                                                                    cttgctgtacatgtcgtgtttttttttctttctgcgccgTCCAATTTTGCTTCCTGCTTTAGCATTTCAGACAACACGGTAATGACAACTTCCCGCGATGTGCTTGATACGCTCGGCTCCTTTTAACATCCGTCTGAGGTATTGGTGGCT                                                 <  SRR19973066.2847803/111‑1 (MQ=49)
                                                                                                    CTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCT                                                 <  SRR19973066.1417687/150‑1 (MQ=60)
                                                                                                     TGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTC                                                >  SRR19973066.1701622/1‑150 (MQ=60)
                                                                                                     TGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTC                                                >  SRR19973066.344573/1‑150 (MQ=60)
                                                                                                     TGGCTGAAGATCGCGGGCTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTC                                                >  SRR19973066.1249763/1‑150 (MQ=60)
                                                                                                      GGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCT                                               <  SRR19973066.1233534/150‑1 (MQ=60)
                                                                                                        CTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGG                                             >  SRR19973066.3143814/1‑150 (MQ=60)
                                                                                                         TGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGG                                            >  SRR19973066.3023428/1‑150 (MQ=60)
                                                                                                         TGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGG                                            >  SRR19973066.3023435/1‑150 (MQ=60)
                                                                                                            AGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTA                                         >  SRR19973066.1697596/1‑150 (MQ=60)
                                                                                                            AGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTA                                         <  SRR19973066.3099693/150‑1 (MQ=60)
                                                                                                              ATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATG                                       >  SRR19973066.3197784/1‑150 (MQ=60)
                                                                                                              ATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATG                                       >  SRR19973066.3197366/1‑150 (MQ=60)
                                                                                                                 GCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCT                                    <  SRR19973066.2455137/150‑1 (MQ=60)
                                                                                                                 GCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCT                                    <  SRR19973066.2152976/150‑1 (MQ=60)
                                                                                                                 GCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCT                                    >  SRR19973066.902889/1‑150 (MQ=60)
                                                                                                                  CGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTC                                   >  SRR19973066.1655912/1‑150 (MQ=60)
                                                                                                                   GGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCC                                  >  SRR19973066.3414231/1‑150 (MQ=60)
                                                                                                                   GGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCC                                  >  SRR19973066.3411950/1‑150 (MQ=60)
                                                                                                                   GGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCC                                  >  SRR19973066.2972609/1‑150 (MQ=60)
                                                                                                                     GTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTG                                <  SRR19973066.2793281/150‑1 (MQ=60)
                                                                                                                     GTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTG                                <  SRR19973066.2693540/150‑1 (MQ=60)
                                                                                                                      TTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGG                               <  SRR19973066.3024387/150‑1 (MQ=60)
                                                                                                                      TTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGG                               <  SRR19973066.2839984/150‑1 (MQ=60)
                                                                                                                       TTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGG                              >  SRR19973066.893010/1‑150 (MQ=60)
                                                                                                                             TGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAG                        >  SRR19973066.3308836/1‑150 (MQ=60)
                                                                                                                             TGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAG                        <  SRR19973066.2985656/150‑1 (MQ=60)
                                                                                                                                ATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTC                     <  SRR19973066.2572800/150‑1 (MQ=60)
                                                                                                                                 TACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAACCGCTCGGCACCGATAAACAACCGTCTGACTTCTTGGTGGCTCTGGGTTATGCCTCCTGCGAGAAAGGTCA                    >  SRR19973066.2255519/1‑150 (MQ=60)
                                                                                                                                 TACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCA                    <  SRR19973066.3080985/150‑1 (MQ=60)
                                                                                                                                 TACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCA                    <  SRR19973066.3081669/150‑1 (MQ=60)
                                                                                                                                   CTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAC                  <  SRR19973066.2801739/150‑1 (MQ=60)
                                                                                                                                   CTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAC                  <  SRR19973066.1756520/150‑1 (MQ=60)
                                                                                                                                   CTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAC                  >  SRR19973066.1901467/1‑150 (MQ=60)
                                                                                                                                   CTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAC                  >  SRR19973066.1265714/1‑150 (MQ=60)
                                                                                                                                   CTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAC                  >  SRR19973066.1901609/1‑150 (MQ=60)
                                                                                                                                    TCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACT                 <  SRR19973066.1268911/150‑1 (MQ=60)
                                                                                                                                    TCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACT                 <  SRR19973066.3330063/150‑1 (MQ=60)
                                                                                                                                     CCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTG                <  SRR19973066.2296201/150‑1 (MQ=60)
                                                                                                                                     CCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTG                <  SRR19973066.1378018/150‑1 (MQ=60)
                                                                                                                                     CCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGGAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTG                <  SRR19973066.539169/150‑1 (MQ=60)
                                                                                                                                         CCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAAC            >  SRR19973066.2035098/1‑150 (MQ=60)
                                                                                                                                         CCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAAC            >  SRR19973066.2655279/1‑150 (MQ=60)
                                                                                                                                          CTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACA           >  SRR19973066.3027006/1‑150 (MQ=60)
                                                                                                                                           TCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAA          <  SRR19973066.1568991/150‑1 (MQ=60)
                                                                                                                                             CAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGGCTCCTGGGAGAAAGGTCAACTGGAACAAGA        <  SRR19973066.753356/150‑1 (MQ=60)
                                                                                                                                                TTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT     <  SRR19973066.1118617/150‑1 (MQ=60)
                                                                                                                                                TTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT     <  SRR19973066.1192271/150‑1 (MQ=60)
                                                                                                                                                TTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAT     <  SRR19973066.188621/150‑1 (MQ=60)
                                                                                                                                                  TTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTC   <  SRR19973066.3207284/150‑1 (MQ=60)
                                                                                                                                                  TAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTC   <  SRR19973066.112799/150‑1 (MQ=60)
                                                                                                                                                   AGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTCT  <  SRR19973066.708150/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTCT  >  CP009273/3086427‑3086723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑