Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,086,574 T→A 92.2% F93L (TTT→TTA)  yqgE → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,086,5740TA92.2% 661.3 / 37.2 219F93L (TTT→TTA) yqgEuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (10/7);  new base A (98/104);  total (108/111)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 219 total aligned reads displayed.

ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGA  >  CP009273/3086429‑3086717
                                                                                                                                                 |                                                                                                                                               
aTCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGc                                                                                                                                               >  1:2775319/1‑148 (MQ=255)
aTCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGc                                                                                                                                               <  2:1434690/148‑1 (MQ=255)
aTCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGc                                                                                                                                               <  1:1396117/148‑1 (MQ=255)
    cAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTcc                                                                                                                                           <  1:2758274/148‑1 (MQ=255)
      aaaTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAg                                                                                                                                         <  2:2177065/148‑1 (MQ=255)
        aTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCa                                                                                                                                       >  1:1480063/1‑148 (MQ=255)
         ttGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCAt                                                                                                                                      >  2:776410/1‑148 (MQ=255)
         ttGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCAt                                                                                                                                      >  1:571609/1‑148 (MQ=255)
         ttGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCAt                                                                                                                                      <  2:1305656/148‑1 (MQ=255)
         ttGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCAt                                                                                                                                      <  2:1685150/148‑1 (MQ=255)
            aaGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCg                                                                                                                                   >  1:24180/1‑148 (MQ=255)
            aaGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCg                                                                                                                                   <  2:1596182/148‑1 (MQ=255)
              gggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCa                                                                                                                                 >  2:2769507/1‑148 (MQ=255)
              gggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCa                                                                                                                                 >  2:1024175/1‑148 (MQ=255)
                 aTTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATCAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATGTAGCTTCCAGCATTCGCAttt                                                                                                                              >  2:1723854/1‑148 (MQ=255)
                  ttCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTc                                                                                                                             <  2:632271/148‑1 (MQ=255)
                   tCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCa                                                                                                                            >  2:2393768/1‑148 (MQ=255)
                      ggAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGac                                                                                                                         >  2:2607807/1‑148 (MQ=255)
                         aaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaac                                                                                                                      >  1:1061695/1‑148 (MQ=255)
                         aaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGacaac                                                                                                                      <  2:2421296/148‑1 (MQ=255)
                         aaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGacaac                                                                                                                      <  1:78242/148‑1 (MQ=255)
                           aGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAacac                                                                                                                    <  2:1857091/148‑1 (MQ=255)
                            gCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACg                                                                                                                   >  2:1155519/1‑148 (MQ=255)
                            gCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACg                                                                                                                   <  2:2576569/148‑1 (MQ=255)
                            gCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACg                                                                                                                   <  2:2037516/148‑1 (MQ=255)
                                      aCGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCa                                                                                                         >  1:3215432/1‑148 (MQ=255)
                                         ccGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACtt                                                                                                      <  2:1628237/148‑1 (MQ=255)
                                         ccGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACtt                                                                                                      <  1:2326263/148‑1 (MQ=255)
                                           ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTAGGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTcc                                                                                                    <  1:930771/148‑1 (MQ=255)
                                            gAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTccc                                                                                                   >  2:2468223/1‑148 (MQ=255)
                                            gAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTccc                                                                                                   >  2:509794/1‑148 (MQ=255)
                                             aGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcg                                                                                                  <  2:2884058/148‑1 (MQ=255)
                                               ccGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcgcg                                                                                                >  1:2209103/1‑148 (MQ=255)
                                                 gcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAt                                                                                              <  1:633142/148‑1 (MQ=255)
                                                  cgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtg                                                                                             <  1:1273296/148‑1 (MQ=255)
                                                    tgatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtgtg                                                                                           >  1:2796883/1‑148 (MQ=255)
                                                     gatgaATCACTCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGc                                                                                          <  2:1043743/148‑1 (MQ=255)
                                                     gatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGc                                                                                          >  2:348768/1‑148 (MQ=255)
                                                      atgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCt                                                                                         >  2:37857/1‑148 (MQ=255)
                                                      atgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCt                                                                                         <  1:482069/148‑1 (MQ=255)
                                                       tgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTg                                                                                        >  1:2795495/1‑148 (MQ=255)
                                                       tgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTg                                                                                        >  1:3440535/1‑148 (MQ=255)
                                                          atcaatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGaa                                                                                     <  1:242126/148‑1 (MQ=255)
                                                           tcaatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTAACCTTCCAGCATTCGCATTTCAGACAACACGGTAAAGACCACTTCCAGCGATGTGCTGGaaa                                                                                    >  2:1968263/1‑148 (MQ=255)
                                                               tcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACACt                                                                                >  1:2551584/1‑148 (MQ=255)
                                                                  gTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCgg                                                                             >  1:2933794/1‑148 (MQ=255)
                                                                        aTAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGa                                                                       <  2:13867/148‑1 (MQ=255)
                                                                         tAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGAt                                                                      <  1:2405720/148‑1 (MQ=255)
                                                                          aaaCCGGTTATGCTCGGCGGACCGCTGGCTGAAGATCGCTGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGCTAATGACCACTTCCCGCGATGTGCTGGACACGCACGGCACCGATa                                                                     >  2:2100086/1‑148 (MQ=255)
                                                                           aaCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATaa                                                                    >  1:1130636/1‑148 (MQ=255)
                                                                             ccGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaac                                                                  <  1:96233/148‑1 (MQ=255)
                                                                              cGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaaca                                                                 <  1:1673765/148‑1 (MQ=255)
                                                                              cGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaaca                                                                 <  1:2549751/148‑1 (MQ=255)
                                                                               ggTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaacaa                                                                <  1:2387528/148‑1 (MQ=255)
                                                                                gTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaacaac                                                               <  1:1024990/148‑1 (MQ=255)
                                                                                  tATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCg                                                             <  1:2904627/148‑1 (MQ=255)
                                                                                  tATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCg                                                             <  1:1153558/148‑1 (MQ=255)
                                                                                  tATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCg                                                             <  1:3103174/148‑1 (MQ=255)
                                                                                   aggCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGt                                                            <  1:299400/146‑1 (MQ=255)
                                                                                    tGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTc                                                           >  1:1511221/1‑148 (MQ=255)
                                                                                          gcggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGt                                                     <  2:1886672/148‑1 (MQ=255)
                                                                                          gcggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGt                                                     >  1:2231297/1‑148 (MQ=255)
                                                                                           cggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTa                                                    >  1:185845/1‑148 (MQ=255)
                                                                                            ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAt                                                   <  1:331527/148‑1 (MQ=255)
                                                                                              tCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtg                                                 >  1:1953127/1‑148 (MQ=255)
                                                                                              tCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtg                                                 <  2:2231297/148‑1 (MQ=255)
                                                                                              tCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtg                                                 <  1:1758311/148‑1 (MQ=255)
                                                                                                    ggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGct                                           <  1:509794/148‑1 (MQ=255)
                                                                                                     gctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGGATTGGTGGctc                                          >  2:3272755/1‑148 (MQ=255)
                                                                                                      ctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctct                                         <  2:3124223/148‑1 (MQ=255)
                                                                                                      ctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctct                                         <  1:348768/148‑1 (MQ=255)
                                                                                                        gAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                       >  1:2702968/1‑148 (MQ=255)
                                                                                                        gAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                       <  2:2339650/148‑1 (MQ=255)
                                                                                                         aaGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTATggg                                      <  1:234968/148‑1 (MQ=255)
                                                                                                           gATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGtt                                    <  1:1866778/148‑1 (MQ=255)
                                                                                                           gATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGtt                                    <  1:2597552/148‑1 (MQ=255)
                                                                                                           gATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGtt                                    >  2:2587035/1‑148 (MQ=255)
                                                                                                            aTCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTa                                   >  1:4191/1‑148 (MQ=255)
                                                                                                              cgcgGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATg                                 >  1:1898179/1‑148 (MQ=255)
                                                                                                              cgcgGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTACAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTATGGGTTATg                                 <  1:1086008/148‑1 (MQ=255)
                                                                                                               gcgGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGc                                <  2:2419199/148‑1 (MQ=255)
                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                             <  2:725460/148‑1 (MQ=255)
                                                                                                                  ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                             <  2:3119683/148‑1 (MQ=255)
                                                                                                                     ttATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTg                          >  2:1563706/1‑148 (MQ=255)
                                                                                                                     ttATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTg                          >  2:1194686/1‑148 (MQ=255)
                                                                                                                     ttATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTg                          >  1:2294033/1‑148 (MQ=255)
                                                                                                                      tATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCAACGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTgg                         >  2:1631559/1‑148 (MQ=255)
                                                                                                                         tttGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGgag                      >  2:405827/1‑148 (MQ=255)
                                                                                                                            gCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGaaa                   <  1:853505/148‑1 (MQ=255)
                                                                                                                              aTACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAgg                 <  2:1061958/148‑1 (MQ=255)
                                                                                                                              aTACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAgg                 >  2:1917498/1‑148 (MQ=255)
                                                                                                                               tACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGt                >  2:1423060/1‑148 (MQ=255)
                                                                                                                               tACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGt                >  2:269406/1‑148 (MQ=255)
                                                                                                                                aCTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTc               >  2:1073427/1‑148 (MQ=255)
                                                                                                                                   ccGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAc            >  1:437124/1‑148 (MQ=255)
                                                                                                                                      cccTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTgg         >  2:1398601/1‑148 (MQ=255)
                                                                                                                                       ccTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGa        <  2:56422/148‑1 (MQ=255)
                                                                                                                                        cTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGaa       >  1:101214/1‑148 (MQ=255)
                                                                                                                                        cTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGaa       >  2:3219190/1‑148 (MQ=255)
                                                                                                                                            aaTTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAg   <  1:2088488/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGA  >  CP009273/3086429‑3086717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATT  >  CP009273/3086425‑3086721
                                                                                                                                                     |                                                                                                                                                   
GAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTA                                                                                                                                                     <  SRR19973065.140722/150‑1 (MQ=60)
 AAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAG                                                                                                                                                    >  SRR19973065.1678033/1‑150 (MQ=60)
  AAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGC                                                                                                                                                   >  SRR19973065.2792548/1‑150 (MQ=60)
   AATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCT                                                                                                                                                  <  SRR19973065.2236651/150‑1 (MQ=60)
    ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTT                                                                                                                                                 <  SRR19973065.1405606/150‑1 (MQ=60)
    ATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTT                                                                                                                                                 <  SRR19973065.1405282/150‑1 (MQ=60)
      CTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCC                                                                                                                                               >  SRR19973065.345702/1‑150 (MQ=60)
       TCAAACTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCA                                                                                                                                              <  SRR19973065.2519936/150‑1 (MQ=60)
        CAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAG                                                                                                                                             <  SRR19973065.546203/150‑1 (MQ=60)
        CAAAATGGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAG                                                                                                                                             <  SRR19973065.2775427/150‑1 (MQ=60)
         AAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGC                                                                                                                                            >  SRR19973065.402357/1‑150 (MQ=60)
          AAATTGAAGGGATTCTGGAAAAGCTGAAGATCTCGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGTCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCCGCA                                                                                                                                           <  SRR19973065.713233/150‑1 (MQ=60)
          AAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCA                                                                                                                                           >  SRR19973065.1489520/1‑150 (MQ=60)
           AATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCAT                                                                                                                                          >  SRR19973065.575218/1‑150 (MQ=60)
              TGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCG                                                                                                                                       >  SRR19973065.24502/1‑150 (MQ=60)
                AAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCA                                                                                                                                     >  SRR19973065.1861615/1‑150 (MQ=60)
                   GGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTT                                                                                                                                  <  SRR19973065.59314/150‑1 (MQ=60)
                     ATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCA                                                                                                                                >  SRR19973065.1068997/1‑150 (MQ=60)
                      TTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAG                                                                                                                               <  SRR19973065.3089433/150‑1 (MQ=60)
                        CTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGAC                                                                                                                             <  SRR19973065.463703/150‑1 (MQ=60)
                        CTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGAC                                                                                                                             >  SRR19973065.243611/1‑150 (MQ=60)
                        CTGGAAAAGCTGAAGATCACGCCGGAGCCGCGAGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGAC                                                                                                                             >  SRR19973065.1876946/1‑150 (MQ=60)
                           GAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAAC                                                                                                                          >  SRR19973065.1068734/1‑150 (MQ=60)
                             AAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACAC                                                                                                                        <  SRR19973065.2935750/150‑1 (MQ=60)
                             AAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACAC                                                                                                                        <  SRR19973065.79033/150‑1 (MQ=60)
                             AAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACAC                                                                                                                        <  SRR19973065.312541/150‑1 (MQ=60)
                               AGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGG                                                                                                                      >  SRR19973065.2236510/1‑150 (MQ=60)
                                 CTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTA                                                                                                                    >  SRR19973065.1935413/1‑150 (MQ=60)
                                    AAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATG                                                                                                                 >  SRR19973065.312023/1‑150 (MQ=60)
                                     AGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGA                                                                                                                <  SRR19973065.1039642/150‑1 (MQ=60)
                                        TCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCA                                                                                                             >  SRR19973065.3234770/1‑150 (MQ=60)
                                        TCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCA                                                                                                             >  SRR19973065.1904089/1‑150 (MQ=60)
                                            GCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTC                                                                                                         >  SRR19973065.3308564/1‑150 (MQ=60)
                                            GCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTC                                                                                                         <  SRR19973065.1212654/150‑1 (MQ=60)
                                             CCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCC                                                                                                        <  SRR19973065.2340822/150‑1 (MQ=60)
                                               GGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCG                                                                                                      >  SRR19973065.1152779/1‑150 (MQ=60)
                                               GGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCG                                                                                                      <  SRR19973065.936912/150‑1 (MQ=60)
                                               GGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTAGGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCG                                                                                                      <  SRR19973065.936901/150‑1 (MQ=60)
                                                GAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGC                                                                                                     >  SRR19973065.2087445/1‑150 (MQ=60)
                                                GAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGC                                                                                                     >  SRR19973065.818672/1‑150 (MQ=60)
                                                GAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGC                                                                                                     >  SRR19973065.133549/1‑150 (MQ=60)
                                                GAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGC                                                                                                     >  SRR19973065.2588937/1‑150 (MQ=60)
                                                 AGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCG                                                                                                    >  SRR19973065.2223229/1‑150 (MQ=60)
                                                     GCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGT                                                                                                <  SRR19973065.638403/150‑1 (MQ=60)
                                                     GCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGT                                                                                                <  SRR19973065.637267/150‑1 (MQ=60)
                                                      CGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTG                                                                                               <  SRR19973065.1281691/150‑1 (MQ=60)
                                                      CGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTG                                                                                               >  SRR19973065.2812978/1‑150 (MQ=60)
                                                      CGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTG                                                                                               >  SRR19973065.2814210/1‑150 (MQ=60)
                                                       GTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGC                                                                                              >  SRR19973065.976865/1‑150 (MQ=60)
                                                       GTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGC                                                                                              >  SRR19973065.23718/1‑150 (MQ=60)
                                                         GATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTG                                                                                            >  SRR19973065.2812816/1‑150 (MQ=60)
                                                         GATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTG                                                                                            >  SRR19973065.2812800/1‑150 (MQ=60)
                                                         GATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTG                                                                                            >  SRR19973065.3461519/1‑150 (MQ=60)
                                                          ATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGG                                                                                           <  SRR19973065.485350/150‑1 (MQ=60)
                                                           TGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGA                                                                                          >  SRR19973065.2337855/1‑150 (MQ=60)
                                                              ATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAC                                                                                       <  SRR19973065.243850/150‑1 (MQ=60)
                                                                 AATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGTAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACACT                                                                                    >  SRR19973065.2567550/1‑150 (MQ=60)
                                                                 AATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACACT                                                                                    >  SRR19973065.2567167/1‑150 (MQ=60)
                                                                 AATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACACT                                                                                    >  SRR19973065.2567554/1‑150 (MQ=60)
                                                                   TCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCG                                                                                  <  SRR19973065.2011059/150‑1 (MQ=60)
                                                                    CCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGG                                                                                 >  SRR19973065.2951589/1‑150 (MQ=60)
                                                                      GTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCA                                                                               >  SRR19973065.1766057/1‑150 (MQ=60)
                                                                           GATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGAT                                                                          >  SRR19973065.2402076/1‑150 (MQ=60)
                                                                             TAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAA                                                                        >  SRR19973065.1137982/1‑150 (MQ=60)
                                                                             TAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAA                                                                        <  SRR19973065.2420856/150‑1 (MQ=60)
                                                                             GAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATCA                                                                        >  SRR19973065.1137960/1‑150 (MQ=60)
                                                                                ACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACA                                                                     <  SRR19973065.432556/150‑1 (MQ=60)
                                                                                 CCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAA                                                                    <  SRR19973065.97129/150‑1 (MQ=60)
                                                                                  CGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAAC                                                                   <  SRR19973065.1684959/150‑1 (MQ=60)
                                                                                  CGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAAC                                                                   <  SRR19973065.2565711/150‑1 (MQ=60)
                                                                                   GGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACC                                                                  <  SRR19973065.1658045/150‑1 (MQ=60)
                                                                                   GGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACC                                                                  <  SRR19973065.2402505/150‑1 (MQ=60)
                                                                                    GTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCG                                                                 <  SRR19973065.1031774/150‑1 (MQ=60)
                                                                                    GTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCG                                                                 <  SRR19973065.1030954/150‑1 (MQ=60)
                                                                                    GTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCG                                                                 <  SRR19973065.2786710/150‑1 (MQ=60)
                                                                                     TTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGT                                                                <  SRR19973065.434530/150‑1 (MQ=60)
                                                                                      TATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTC                                                               >  SRR19973065.1521064/1‑150 (MQ=60)
                                                                                      TATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTC                                                               >  SRR19973065.1520988/1‑150 (MQ=60)
                                                                                      TATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTC                                                               <  SRR19973065.2922335/150‑1 (MQ=60)
                                                                                      TATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTC                                                               <  SRR19973065.1160974/150‑1 (MQ=60)
                                                                                      TATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTC                                                               <  SRR19973065.3122108/150‑1 (MQ=60)
                                                                                       AGGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCT                                                              <  SRR19973065.301340/150‑1 (MQ=60)
                                                                                            CGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGT                                                         >  SRR19973065.2245495/1‑150 (MQ=60)
                                                                                             GGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTA                                                        >  SRR19973065.187305/1‑150 (MQ=60)
                                                                                                GGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTG                                                     >  SRR19973065.1965889/1‑150 (MQ=60)
                                                                                                GGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTG                                                     <  SRR19973065.333848/150‑1 (MQ=60)
                                                                                                  TCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGT                                                   <  SRR19973065.1769864/150‑1 (MQ=60)
                                                                                                        GGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCT                                             <  SRR19973065.513176/150‑1 (MQ=60)
                                                                                                        GGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCT                                             <  SRR19973065.2408811/150‑1 (MQ=60)
                                                                                                          CTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGG                                           <  SRR19973065.351213/150‑1 (MQ=60)
                                                                                                          CTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGG                                           >  SRR19973065.2719771/1‑150 (MQ=60)
                                                                                                             AAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTT                                        <  SRR19973065.215948/150‑1 (MQ=60)
                                                                                                             AAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTATGGGTT                                        <  SRR19973065.236667/150‑1 (MQ=60)
                                                                                                              AGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTA                                       >  SRR19973065.4247/1‑150 (MQ=60)
                                                                                                              AGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTA                                       <  SRR19973065.2373349/150‑1 (MQ=60)
                                                                                                               GATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTAT                                      <  SRR19973065.1879065/150‑1 (MQ=60)
                                                                                                               GATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTAT                                      <  SRR19973065.2613695/150‑1 (MQ=60)
                                                                                                                ATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATG                                     >  SRR19973065.1910654/1‑150 (MQ=60)
                                                                                                                ATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATG                                     <  SRR19973065.2980190/150‑1 (MQ=60)
                                                                                                                  CGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCC                                   <  SRR19973065.3476596/150‑1 (MQ=60)
                                                                                                                  CGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTACAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTATGGGTTATGCC                                   <  SRR19973065.1093167/150‑1 (MQ=60)
                                                                                                                   GCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCT                                  >  SRR19973065.1708789/1‑150 (MQ=60)
                                                                                                                    CGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTC                                 <  SRR19973065.2623987/150‑1 (MQ=60)
                                                                                                                    CGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTC                                 >  SRR19973065.2757576/1‑150 (MQ=60)
                                                                                                                     GGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCC                                >  SRR19973065.2792620/1‑150 (MQ=60)
                                                                                                                       GTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTG                              >  SRR19973065.2308485/1‑150 (MQ=60)
                                                                                                                                GCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGG                     >  SRR19973065.2422144/1‑150 (MQ=60)
                                                                                                                                GCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGG                     <  SRR19973065.859027/150‑1 (MQ=60)
                                                                                                                                GCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGG                     <  SRR19973065.859490/150‑1 (MQ=60)
                                                                                                                                   TACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCA                  <  SRR19973065.2900070/150‑1 (MQ=60)
                                                                                                                                     CTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAAC                >  SRR19973065.440156/1‑150 (MQ=60)
                                                                                                                                      TCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACT               >  SRR19973065.506881/1‑150 (MQ=60)
                                                                                                                                      TCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACT               >  SRR19973065.2287550/1‑150 (MQ=60)
                                                                                                                                          CCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAA           >  SRR19973065.102145/1‑150 (MQ=60)
                                                                                                                                                AATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAA     <  SRR19973065.2101890/150‑1 (MQ=60)
                                                                                                                                                 ATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAA    <  SRR19973065.1276931/150‑1 (MQ=60)
                                                                                                                                                   TTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATT  >  SRR19973065.3104923/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GAAAATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATT  >  CP009273/3086425‑3086721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑