Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,135,168 G→T 94.2% V416L (GTA→TTA)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,135,1680GT94.2% 512.3 / 21.4 182V416L (GTA→TTA) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (5/6);  new base T (99/72);  total (104/78)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 182 total aligned reads displayed.

ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  CP009273/4135022‑4135312
                                                                                                                                                  |                                                                                                                                                
  gCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAg                                                                                                                                               <  2:3008152/148‑1 (MQ=255)
   ccTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGt                                                                                                                                              <  1:3051988/148‑1 (MQ=255)
      tGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTggg                                                                                                                                           >  2:460578/1‑148 (MQ=255)
          cGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgt                                                                                                                                       <  1:2976681/148‑1 (MQ=255)
            atacGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtgtgt                                                                                                                                     <  2:1849099/146‑1 (MQ=255)
            acacGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgtgt                                                                                                                                     >  2:2287916/1‑148 (MQ=255)
                 ccGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAt                                                                                                                                >  2:1320371/1‑148 (MQ=255)
                 ccGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAt                                                                                                                                >  2:742536/1‑148 (MQ=255)
                 ccGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAAt                                                                                                                                >  1:2895022/1‑148 (MQ=255)
                  cGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAtt                                                                                                                               <  1:1718502/148‑1 (MQ=255)
                   gTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTa                                                                                                                              >  2:3504750/1‑148 (MQ=255)
                         gatgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAtt                                                                                                                        <  1:951869/148‑1 (MQ=255)
                          atgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTc                                                                                                                       <  2:2529528/148‑1 (MQ=255)
                              aaaCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCgg                                                                                                                   >  1:840241/1‑148 (MQ=255)
                              aaaCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCgg                                                                                                                   >  2:3154463/1‑148 (MQ=255)
                                aCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                                                                                                 >  2:1802431/1‑148 (MQ=255)
                                aCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                                                                                                 >  2:1031285/1‑148 (MQ=255)
                                 ccgccgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg                                                                                                                >  1:773609/1‑148 (MQ=255)
                                  cgccgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGa                                                                                                               <  2:38403/148‑1 (MQ=255)
                                     cgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAAcc                                                                                                            <  2:111733/148‑1 (MQ=255)
                                     cgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAAcc                                                                                                            <  2:1915162/148‑1 (MQ=255)
                                      gAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                           >  2:3199454/1‑148 (MQ=255)
                                        aaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAc                                                                                                         >  2:2691351/1‑148 (MQ=255)
                                        aaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAc                                                                                                         >  2:2969693/1‑148 (MQ=255)
                                        aaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAc                                                                                                         <  1:1747220/148‑1 (MQ=255)
                                          ccATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACgg                                                                                                       >  1:1249686/1‑148 (MQ=255)
                                          ccATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACgg                                                                                                       >  2:2349536/1‑148 (MQ=255)
                                            aTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCt                                                                                                     >  1:2022371/1‑148 (MQ=255)
                                            aTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCt                                                                                                     >  1:2892690/1‑148 (MQ=255)
                                            aTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCt                                                                                                     >  1:220944/1‑148 (MQ=255)
                                             ttCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCtt                                                                                                    >  1:3556338/1‑148 (MQ=255)
                                                 cTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTCCGGCTTGCAt                                                                                                >  2:3510878/1‑148 (MQ=255)
                                                 cTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                >  2:3510915/1‑148 (MQ=255)
                                                      gTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                           <  1:443006/148‑1 (MQ=255)
                                                       tACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc                                                                                          >  1:1588198/1‑148 (MQ=255)
                                                          cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                                                                                       >  2:2874689/1‑148 (MQ=255)
                                                            gTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGAt                                                                                     <  1:2349536/148‑1 (MQ=255)
                                                            gTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGAt                                                                                     <  2:34749/148‑1 (MQ=255)
                                                            gTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGAt                                                                                     <  2:1863299/148‑1 (MQ=255)
                                                            gTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGAt                                                                                     <  2:1862733/148‑1 (MQ=255)
                                                             tATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATg                                                                                    >  2:2349333/1‑148 (MQ=255)
                                                             tATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATg                                                                                    <  2:3130124/148‑1 (MQ=255)
                                                              aTTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                   >  1:55690/1‑148 (MQ=255)
                                                              aTTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                   >  1:55892/1‑148 (MQ=255)
                                                              aTTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                   <  2:2937912/148‑1 (MQ=255)
                                                                 ccGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTa                                                                                <  2:1613071/148‑1 (MQ=255)
                                                                   gCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                              <  1:1320371/148‑1 (MQ=255)
                                                                   gCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                              <  1:742536/148‑1 (MQ=255)
                                                                    cAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATc                                                                             <  1:187521/148‑1 (MQ=255)
                                                                     aaaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATct                                                                            >  2:1268034/1‑148 (MQ=255)
                                                                       aTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctgc                                                                          >  1:2653764/1‑148 (MQ=255)
                                                                        tCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctgct                                                                         >  1:67427/1‑148 (MQ=255)
                                                                            tAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGaaa                                                                     <  2:2638899/148‑1 (MQ=255)
                                                                            tAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGaaa                                                                     <  2:851510/148‑1 (MQ=255)
                                                                             aaCGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAg                                                                    >  2:136426/1‑148 (MQ=255)
                                                                               cGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTg                                                                  <  2:1350038/148‑1 (MQ=255)
                                                                               cGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTg                                                                  >  1:1605895/1‑148 (MQ=255)
                                                                                 aTGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                <  1:2718946/148‑1 (MQ=255)
                                                                                   gAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATgg                                                              >  1:27617/1‑148 (MQ=255)
                                                                                   gAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATgg                                                              >  1:1986214/1‑148 (MQ=255)
                                                                                    aaGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGa                                                             >  1:3253282/1‑148 (MQ=255)
                                                                                     aGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaa                                                            >  2:2654964/1‑148 (MQ=255)
                                                                                      gtggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaaa                                                           >  1:2575932/1‑148 (MQ=255)
                                                                                       tggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                          >  1:2036186/1‑148 (MQ=255)
                                                                                        ggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATc                                                         <  2:2508186/148‑1 (MQ=255)
                                                                                           ggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATctgc                                                      <  2:67427/148‑1 (MQ=255)
                                                                                           ggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATctgc                                                      <  2:2989073/148‑1 (MQ=255)
                                                                                                 aGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCAt                                                >  1:2876805/1‑148 (MQ=255)
                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATg                                               >  2:1102398/1‑148 (MQ=255)
                                                                                                     ttCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGaaa                                            >  1:2291940/1‑148 (MQ=255)
                                                                                                      tCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGaaaa                                           >  1:338874/1‑148 (MQ=255)
                                                                                                        cTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATg                                         >  1:2900370/1‑148 (MQ=255)
                                                                                                         tCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGa                                        <  1:923482/148‑1 (MQ=255)
                                                                                                          cAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGaa                                       <  2:2028844/148‑1 (MQ=255)
                                                                                                          cAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGaa                                       >  2:3238235/1‑148 (MQ=255)
                                                                                                            aCCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATATGCCTGCATGAAAATGAAgg                                     >  2:3489336/1‑148 (MQ=255)
                                                                                                               gTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCaa                                  >  1:683216/1‑148 (MQ=255)
                                                                                                                tGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAt                                 >  1:307202/1‑148 (MQ=255)
                                                                                                                 ggCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAATGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtg                                <  2:2665806/148‑1 (MQ=255)
                                                                                                                 ggCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtg                                >  2:1632372/1‑148 (MQ=255)
                                                                                                                     tttCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtgctgc                            >  1:3370338/1‑148 (MQ=255)
                                                                                                                      ttCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTgcg                           >  1:3040509/1‑148 (MQ=255)
                                                                                                                        cGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCt                         <  1:460578/148‑1 (MQ=255)
                                                                                                                          cTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGa                       >  1:2218408/1‑148 (MQ=255)
                                                                                                                           tGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGAc                      <  2:27617/148‑1 (MQ=255)
                                                                                                                           tGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGAc                      <  2:1458492/148‑1 (MQ=255)
                                                                                                                            ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACt                     >  2:3037812/1‑148 (MQ=255)
                                                                                                                             gAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACtt                    >  2:486954/1‑148 (MQ=255)
                                                                                                                                 aTGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGa                >  2:802994/1‑148 (MQ=255)
                                                                                                                                   gcgcgcGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg              >  1:603037/1‑148 (MQ=255)
                                                                                                                                     gcgcgACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGt            <  2:970123/148‑1 (MQ=255)
                                                                                                                                       gcgACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGttt          <  1:2568268/148‑1 (MQ=255)
                                                                                                                                        cgACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATATGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTa         <  1:3232619/148‑1 (MQ=255)
                                                                                                                                        cgACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTa         <  2:2895022/148‑1 (MQ=255)
                                                                                                                                           cTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg      >  1:1504880/1‑148 (MQ=255)
                                                                                                                                              ttCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAg   <  1:717995/148‑1 (MQ=255)
                                                                                                                                               tCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGatc  >  1:3224155/1‑146 (MQ=255)
                                                                                                                                               tCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGatc  >  1:3224307/1‑146 (MQ=255)
                                                                                                                                               tCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGc  >  1:2395433/1‑148 (MQ=255)
                                                                                                                                               tCCTTAGTGGGCTGTGTGGAATTATCTAGTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGc  <  2:588576/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  CP009273/4135022‑4135312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  >  CP009273/4135020‑4135316
                                                                                                                                                    |                                                                                                                                                    
TAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTT                                                                                                                                                     <  SRR19973066.3313933/150‑1 (MQ=60)
 AACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTA                                                                                                                                                    <  SRR19973066.626763/150‑1 (MQ=60)
 AACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTA                                                                                                                                                    <  SRR19973066.626698/150‑1 (MQ=60)
  ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAG                                                                                                                                                   <  SRR19973066.2689963/150‑1 (MQ=60)
   CGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGT                                                                                                                                                  <  SRR19973066.2119210/150‑1 (MQ=60)
   CGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGT                                                                                                                                                  <  SRR19973066.202671/150‑1 (MQ=60)
     CCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGG                                                                                                                                                <  SRR19973066.3072399/150‑1 (MQ=60)
            CGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGT                                                                                                                                         <  SRR19973066.2996352/150‑1 (MQ=60)
                 CGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAAT                                                                                                                                    >  SRR19973066.2914374/1‑150 (MQ=60)
                    CGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTAT                                                                                                                                 <  SRR19973066.1730953/150‑1 (MQ=60)
                     GTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATC                                                                                                                                >  SRR19973066.407218/1‑150 (MQ=60)
                     GTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATC                                                                                                                                >  SRR19973066.1850087/1‑150 (MQ=60)
                     GTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATC                                                                                                                                >  SRR19973066.2673572/1‑150 (MQ=60)
                      TTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCT                                                                                                                               >  SRR19973066.492684/1‑150 (MQ=60)
                          TGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGACGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTC                                                                                                                           >  SRR19973066.3151019/1‑150 (MQ=60)
                           GATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCC                                                                                                                          <  SRR19973066.958825/150‑1 (MQ=60)
                            ATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCC                                                                                                                         <  SRR19973066.451848/150‑1 (MQ=60)
                              GAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGG                                                                                                                       >  SRR19973066.846352/1‑150 (MQ=60)
                                AAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCA                                                                                                                     <  SRR19973066.169655/150‑1 (MQ=60)
                                 AACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG                                                                                                                    <  SRR19973066.972591/150‑1 (MQ=60)
                                 AACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG                                                                                                                    >  SRR19973066.779331/1‑150 (MQ=60)
                                       CGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTC                                                                                                              >  SRR19973066.3377958/1‑150 (MQ=60)
                                       CGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTA                                                                                                              >  SRR19973066.2425250/1‑150 (MQ=60)
                                        GAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAC                                                                                                             >  SRR19973066.2836534/1‑150 (MQ=60)
                                          AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGG                                                                                                           >  SRR19973066.1258659/1‑150 (MQ=60)
                                          AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGG                                                                                                           <  SRR19973066.1759795/150‑1 (MQ=60)
                                           ACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGC                                                                                                          >  SRR19973066.2406957/1‑150 (MQ=60)
                                           ACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTACGACAACCTTGGCGTTTCGATGGAAGATGCGCGCGACAATACGTTAGTGGGCTGTGTGGAATTATAAATTCCCGGCAGAACCTACGGC                                                                                                          >  SRR19973066.1944419/1‑150 (MQ=60)
                                            CCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCT                                                                                                         >  SRR19973066.222741/1‑150 (MQ=60)
                                            CCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCT                                                                                                         >  SRR19973066.2036517/1‑150 (MQ=60)
                                            CCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCT                                                                                                         >  SRR19973066.2912027/1‑150 (MQ=60)
                                             CATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTT                                                                                                        <  SRR19973066.2341338/150‑1 (MQ=60)
                                             CATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTT                                                                                                        >  SRR19973066.3579926/1‑150 (MQ=60)
                                                       GGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATC                                                                                              >  SRR19973066.1599688/1‑150 (MQ=60)
                                                       GGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATC                                                                                              >  SRR19973066.1599767/1‑150 (MQ=60)
                                                        GTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCG                                                                                             <  SRR19973066.446431/150‑1 (MQ=60)
                                                              GTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGT                                                                                       >  SRR19973066.56384/1‑150 (MQ=60)
                                                              GTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGT                                                                                       <  SRR19973066.2365505/150‑1 (MQ=60)
                                                              GTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGT                                                                                       >  SRR19973066.56589/1‑150 (MQ=60)
                                                                ATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                     >  SRR19973066.646886/1‑150 (MQ=60)
                                                                ATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                     <  SRR19973066.2039446/150‑1 (MQ=60)
                                                                ATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                     >  SRR19973066.897338/1‑150 (MQ=60)
                                                                     GCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCT                                                                                <  SRR19973066.1329944/150‑1 (MQ=60)
                                                                     GCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCT                                                                                <  SRR19973066.748049/150‑1 (MQ=60)
                                                                      CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                               <  SRR19973066.1461874/150‑1 (MQ=60)
                                                                      CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                               >  SRR19973066.2908188/1‑150 (MQ=60)
                                                                      CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                               <  SRR19973066.189145/150‑1 (MQ=60)
                                                                       AAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGC                                                                              >  SRR19973066.2671568/1‑150 (MQ=60)
                                                                        AATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCT                                                                             >  SRR19973066.68243/1‑150 (MQ=60)
                                                                          TCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGA                                                                           >  SRR19973066.300717/1‑150 (MQ=60)
                                                                               AACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTG                                                                      >  SRR19973066.1617546/1‑150 (MQ=60)
                                                                                   ATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGG                                                                  <  SRR19973066.2737228/150‑1 (MQ=60)
                                                                                   ATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGG                                                                  >  SRR19973066.2000216/1‑150 (MQ=60)
                                                                                   ATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGG                                                                  >  SRR19973066.28007/1‑150 (MQ=60)
                                                                                    TGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA                                                                 >  SRR19973066.3274692/1‑150 (MQ=60)
                                                                                    TGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA                                                                 >  SRR19973066.3274705/1‑150 (MQ=60)
                                                                                      AAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAA                                                               >  SRR19973066.2593408/1‑150 (MQ=60)
                                                                                       AGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAT                                                              >  SRR19973066.2050387/1‑150 (MQ=60)
                                                                                                 CCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCAT                                                    >  SRR19973066.2896077/1‑150 (MQ=60)
                                                                                                 CCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCAT                                                    >  SRR19973066.1549117/1‑150 (MQ=60)
                                                                                                     CGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAA                                                >  SRR19973066.2307655/1‑150 (MQ=60)
                                                                                                      GTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAA                                               >  SRR19973066.2917240/1‑150 (MQ=60)
                                                                                                      GTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAA                                               >  SRR19973066.341481/1‑150 (MQ=60)
                                                                                                       TTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAAT                                              <  SRR19973066.3579336/150‑1 (MQ=60)
                                                                                                        TCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATG                                             >  SRR19973066.2919753/1‑150 (MQ=60)
                                                                                                           TCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAG                                          <  SRR19973066.930146/150‑1 (MQ=60)
                                                                                                               CCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAA                                      >  SRR19973066.688239/1‑150 (MQ=60)
                                                                                                               CCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAA                                      >  SRR19973066.688198/1‑150 (MQ=60)
                                                                                                               CCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAA                                      >  SRR19973066.688236/1‑150 (MQ=60)
                                                                                                                CGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAT                                     >  SRR19973066.309425/1‑150 (MQ=60)
                                                                                                                 GTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATG                                    >  SRR19973066.932217/1‑150 (MQ=60)
                                                                                                                    GCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTG                                 <  SRR19973066.2931509/150‑1 (MQ=60)
                                                                                                                     CGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGC                                >  SRR19973066.3392819/1‑150 (MQ=60)
                                                                                                                     CGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGC                                >  SRR19973066.2765578/1‑150 (MQ=60)
                                                                                                                      GTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCG                               >  SRR19973066.306440/1‑150 (MQ=60)
                                                                                                                      GTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCG                               >  SRR19973066.3060827/1‑150 (MQ=60)
                                                                                                                          CGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGA                           <  SRR19973066.464102/150‑1 (MQ=60)
                                                                                                                          CGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGA                           >  SRR19973066.2233759/1‑150 (MQ=60)
                                                                                                                           GCTGGAAGATGCGAGCGACTATTCCTTAGTGGGCTGTGTTGAATTATCTATTACCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGAC                          <  SRR19973066.726541/150‑1 (MQ=60)
                                                                                                                             TGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTT                        >  SRR19973066.2235396/1‑150 (MQ=60)
                                                                                                                                   ATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAG                  >  SRR19973066.607114/1‑150 (MQ=60)
                                                                                                                                   ATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAG                  >  SRR19973066.607189/1‑150 (MQ=60)
                                                                                                                                     GCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAGTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGT                <  SRR19973066.82611/150‑1 (MQ=60)
                                                                                                                                         GCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTAC            <  SRR19973066.2585695/150‑1 (MQ=60)
                                                                                                                                          CGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATATGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACT           <  SRR19973066.3253969/150‑1 (MQ=60)
                                                                                                                                          CGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATATGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACT           <  SRR19973066.3253524/150‑1 (MQ=60)
                                                                                                                                           GACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTG          >  SRR19973066.1515360/1‑150 (MQ=60)
                                                                                                                                               ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGATC      >  SRR19973066.3245628/1‑150 (MQ=60)
                                                                                                                                               ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGATC      >  SRR19973066.3245555/1‑150 (MQ=60)
                                                                                                                                               ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGATC      >  SRR19973066.3245476/1‑150 (MQ=60)
                                                                                                                                               ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC      >  SRR19973066.2608022/1‑150 (MQ=60)
                                                                                                                                               ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC      >  SRR19973066.2411576/1‑150 (MQ=60)
                                                                                                                                                TTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCA     <  SRR19973066.723306/150‑1 (MQ=60)
                                                                                                                                                   CTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  <  SRR19973066.3404255/150‑1 (MQ=60)
                                                                                                                                                   CTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  <  SRR19973066.2050163/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  >  CP009273/4135020‑4135316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑