Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,135,168 G→T 100% V416L (GTA→TTA)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,135,1680GT98.0% 631.3 / 3.6 200V416L (GTA→TTA) pflDputative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (88/108);  total (88/112)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

CGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  CP009273/4135023‑4135312
                                                                                                                                                 |                                                                                                                                                
cGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTa                                                                                                                                                >  1:698293/1‑148 (MQ=255)
 gCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAg                                                                                                                                               <  1:3707289/148‑1 (MQ=255)
     tGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTggg                                                                                                                                           >  1:3008708/1‑148 (MQ=255)
     tGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTggg                                                                                                                                           >  1:3008715/1‑148 (MQ=255)
     tGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTggg                                                                                                                                           <  1:1704785/148‑1 (MQ=255)
        tCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCtg                                                                                                                                        <  2:3757487/148‑1 (MQ=255)
         cGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgt                                                                                                                                       >  1:2951210/1‑148 (MQ=255)
          gACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgtg                                                                                                                                      <  1:2044616/148‑1 (MQ=255)
          gACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgtg                                                                                                                                      <  1:2018178/148‑1 (MQ=255)
           acacGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAGTCCTTAGTGGGCtgtgt                                                                                                                                     <  2:2930600/148‑1 (MQ=255)
           acacGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCCTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgtgt                                                                                                                                     >  2:1294106/1‑148 (MQ=255)
                  gTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGACTTa                                                                                                                              >  1:446546/1‑148 (MQ=255)
                  gTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTa                                                                                                                              >  2:1651383/1‑148 (MQ=255)
                   ttCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTAt                                                                                                                             <  2:340815/148‑1 (MQ=255)
                   ttCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTAt                                                                                                                             <  1:3139263/148‑1 (MQ=255)
                   ttCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTAt                                                                                                                             >  1:2897150/1‑148 (MQ=255)
                     ccTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCt                                                                                                                           <  2:2681020/148‑1 (MQ=255)
                        gatgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAtt                                                                                                                        >  1:1624510/1‑148 (MQ=255)
                        gatgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAtt                                                                                                                        >  1:1474836/1‑148 (MQ=255)
                          tgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTcc                                                                                                                      <  2:1251528/148‑1 (MQ=255)
                          tgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTcc                                                                                                                      >  1:957222/1‑148 (MQ=255)
                            aaaaCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGATGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCg                                                                                                                    >  2:1628813/1‑148 (MQ=255)
                              aaCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAGTATCTATTCCCGGc                                                                                                                  >  2:844806/1‑148 (MQ=255)
                               aCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCa                                                                                                                 >  2:1593050/1‑148 (MQ=255)
                                ccgccgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg                                                                                                                >  2:2042322/1‑148 (MQ=255)
                                 cgccgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATATTTAACGATGAAGTGGTGGTGCCAGCGTTACTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGa                                                                                                               <  1:3658299/148‑1 (MQ=255)
                                      aaaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTa                                                                                                          <  1:3638397/148‑1 (MQ=255)
                                      aaaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTa                                                                                                          <  1:2020157/148‑1 (MQ=255)
                                       aaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAc                                                                                                         <  1:1720289/148‑1 (MQ=255)
                                         ccATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACgg                                                                                                       >  2:1347820/1‑148 (MQ=255)
                                          cATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc                                                                                                      <  1:2429047/148‑1 (MQ=255)
                                          cATTCGCTTCGATACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGc                                                                                                      <  1:2583643/148‑1 (MQ=255)
                                              cGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGc                                                                                                  <  2:1451615/148‑1 (MQ=255)
                                              cGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGc                                                                                                  <  2:1451247/148‑1 (MQ=255)
                                              cGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTGTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAGTAGCTATTCCCGGCAGAACCTACGGCGTGc                                                                                                  <  2:3202563/148‑1 (MQ=255)
                                               gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGAGGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa                                                                                                 <  1:2271545/148‑1 (MQ=255)
                                                 ttCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATg                                                                                               <  2:3538597/148‑1 (MQ=255)
                                                  tCGGTACCGGTATTCCGCATAACTTTAACGATGAAGTTGTGGTGCCAGCGTTCCTCTACCGTGGCGTTTCGCTGGAAGAGGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTTGGGCTTGCATGa                                                                                              <  2:2852011/148‑1 (MQ=255)
                                                  tCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGa                                                                                              <  1:1518743/148‑1 (MQ=255)
                                                  tCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGa                                                                                              >  1:2476882/1‑148 (MQ=255)
                                                   cGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGAc                                                                                             <  2:2350305/148‑1 (MQ=255)
                                                    ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACa                                                                                            <  2:3595455/148‑1 (MQ=255)
                                                    ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACa                                                                                            <  2:956822/148‑1 (MQ=255)
                                                        ccGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                                                                                        >  2:3252081/1‑148 (MQ=255)
                                                         cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                                                                                       <  2:2957873/148‑1 (MQ=255)
                                                         cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                                                                                       <  2:2956942/148‑1 (MQ=255)
                                                         cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                                                                                       <  2:1849150/148‑1 (MQ=255)
                                                                ccGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTa                                                                                <  1:3369453/148‑1 (MQ=255)
                                                                ccGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTa                                                                                <  2:1302553/148‑1 (MQ=255)
                                                                   cAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATc                                                                             <  2:283219/148‑1 (MQ=255)
                                                                    aaaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATct                                                                            >  1:1548560/1‑148 (MQ=255)
                                                                     aaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                           <  2:2214923/148‑1 (MQ=255)
                                                                     aaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                           >  1:3404877/1‑148 (MQ=255)
                                                                     aaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                           <  1:2138405/148‑1 (MQ=255)
                                                                     aaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                           <  2:2764182/148‑1 (MQ=255)
                                                                       tCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctgct                                                                         >  1:2594033/1‑148 (MQ=255)
                                                                       tCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctgct                                                                         >  2:3658825/1‑148 (MQ=255)
                                                                        cTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGACTTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctgctg                                                                        >  1:1781454/1‑148 (MQ=255)
                                                                        cTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctgctg                                                                        >  1:3675305/1‑148 (MQ=255)
                                                                         tttAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa                                                                       >  1:792601/1‑148 (MQ=255)
                                                                          ttAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGaa                                                                      <  1:633887/148‑1 (MQ=255)
                                                                          ttAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGaa                                                                      >  1:1858121/1‑148 (MQ=255)
                                                                            aaCGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAg                                                                    <  1:2326135/148‑1 (MQ=255)
                                                                              cGATGAAGTGGTGGTGCCAGCGTTCCACAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTg                                                                  >  1:2222965/1‑148 (MQ=255)
                                                                                aTGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                >  1:3507133/1‑148 (MQ=255)
                                                                                aTGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                >  1:1823881/1‑148 (MQ=255)
                                                                                   aaGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGa                                                             >  1:1969249/1‑148 (MQ=255)
                                                                                    aGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaa                                                            <  2:3727264/148‑1 (MQ=255)
                                                                                      tggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                          <  2:1239584/148‑1 (MQ=255)
                                                                                      tggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                          <  1:1593050/148‑1 (MQ=255)
                                                                                      tggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                          >  2:47158/1‑148 (MQ=255)
                                                                                          ggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATctgc                                                      <  1:3747446/148‑1 (MQ=255)
                                                                                           gtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATctgcc                                                     <  1:652230/148‑1 (MQ=255)
                                                                                                    ttCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGaaa                                            >  2:1801197/1‑148 (MQ=255)
                                                                                                       cTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATg                                         >  2:3101185/1‑148 (MQ=255)
                                                                                                        tCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGa                                        <  2:3777007/148‑1 (MQ=255)
                                                                                                        tCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGa                                        >  1:890604/1‑148 (MQ=255)
                                                                                                        tCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGa                                        <  2:1515952/148‑1 (MQ=255)
                                                                                                         cAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGaa                                       <  2:525329/148‑1 (MQ=255)
                                                                                                         cAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGaa                                       >  1:1151759/1‑148 (MQ=255)
                                                                                                             cGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                   <  1:412272/148‑1 (MQ=255)
                                                                                                             cGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                   <  1:3315215/148‑1 (MQ=255)
                                                                                                             cGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                   <  1:3315440/148‑1 (MQ=255)
                                                                                                               tGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAt                                 <  1:1995704/148‑1 (MQ=255)
                                                                                                                   gTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtgctg                             <  1:1294379/148‑1 (MQ=255)
                                                                                                                        gCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTg                        <  1:835935/148‑1 (MQ=255)
                                                                                                                         cTGGAAGAGGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATGATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGa                       <  2:1982140/148‑1 (MQ=255)
                                                                                                                           ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACt                     >  2:2573544/1‑148 (MQ=255)
                                                                                                                           ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACt                     >  2:748338/1‑148 (MQ=255)
                                                                                                                           ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACt                     >  2:1335372/1‑148 (MQ=255)
                                                                                                                           ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACt                     >  2:1552365/1‑148 (MQ=255)
                                                                                                                                aTGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGa                >  1:577578/1‑148 (MQ=255)
                                                                                                                                 tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGaa               >  2:112491/1‑148 (MQ=255)
                                                                                                                                 tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGaa               <  2:577578/148‑1 (MQ=255)
                                                                                                                                    gcgcgACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGt            <  2:698293/148‑1 (MQ=255)
                                                                                                                                     cgcgACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGtt           <  2:633276/148‑1 (MQ=255)
                                                                                                                                           tATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTgg     >  2:3275578/1‑148 (MQ=255)
                                                                                                                                            aTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGa    >  2:3581428/1‑148 (MQ=255)
                                                                                                                                            aTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGa    >  2:3581391/1‑148 (MQ=255)
                                                                                                                                            aTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGa    >  1:2900972/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  CP009273/4135023‑4135312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  >  CP009273/4135019‑4135316
                                                                                                                                                     |                                                                                                                                                    
CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCT                                                                                                                                                      <  SRR19973069.2152794/150‑1 (MQ=60)
CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCT                                                                                                                                                      <  SRR19973069.207955/150‑1 (MQ=60)
  AACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTA                                                                                                                                                    >  SRR19973069.703193/1‑150 (MQ=60)
    CGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGT                                                                                                                                                  <  SRR19973069.1386050/150‑1 (MQ=60)
     GCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTG                                                                                                                                                 <  SRR19973069.3731511/150‑1 (MQ=60)
       CTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGG                                                                                                                                               >  SRR19973069.3028669/1‑150 (MQ=60)
       CTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGG                                                                                                                                               >  SRR19973069.3028676/1‑150 (MQ=60)
         TGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCT                                                                                                                                             >  SRR19973069.1527524/1‑150 (MQ=60)
         TGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCT                                                                                                                                             <  SRR19973069.1716728/150‑1 (MQ=60)
         TGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCT                                                                                                                                             <  SRR19973069.1716718/150‑1 (MQ=60)
           ATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGT                                                                                                                                           <  SRR19973069.588245/150‑1 (MQ=60)
           ATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGT                                                                                                                                           >  SRR19973069.2970793/1‑150 (MQ=60)
             CGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGT                                                                                                                                         >  SRR19973069.2023360/1‑150 (MQ=60)
             CGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGT                                                                                                                                         >  SRR19973069.3123361/1‑150 (MQ=60)
              GACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTG                                                                                                                                        <  SRR19973069.2058808/150‑1 (MQ=60)
              GACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTG                                                                                                                                        <  SRR19973069.2032213/150‑1 (MQ=60)
                    CCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGACTTA                                                                                                                                  >  SRR19973069.449814/1‑150 (MQ=60)
                     CGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTAT                                                                                                                                 >  SRR19973069.2916266/1‑150 (MQ=60)
                       TTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCT                                                                                                                               <  SRR19973069.3159821/150‑1 (MQ=60)
                          CTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATT                                                                                                                            >  SRR19973069.1635553/1‑150 (MQ=60)
                          CTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATT                                                                                                                            >  SRR19973069.3049232/1‑150 (MQ=60)
                          CTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATT                                                                                                                            >  SRR19973069.1485109/1‑150 (MQ=60)
                            GATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCC                                                                                                                          >  SRR19973069.963633/1‑150 (MQ=60)
                            GATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCC                                                                                                                          >  SRR19973069.3618925/1‑150 (MQ=60)
                            GATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCC                                                                                                                          >  SRR19973069.963233/1‑150 (MQ=60)
                                     CGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAAC                                                                                                                 <  SRR19973069.3682299/150‑1 (MQ=60)
                                     CGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATATTTAACGATGAAGTGGTGGTGCCAGCGTTACTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAAC                                                                                                                 <  SRR19973069.3682254/150‑1 (MQ=60)
                                          AAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACG                                                                                                            <  SRR19973069.3662212/150‑1 (MQ=60)
                                          AAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACG                                                                                                            <  SRR19973069.2034081/150‑1 (MQ=60)
                                          AAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACG                                                                                                            <  SRR19973069.2034199/150‑1 (MQ=60)
                                           AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGG                                                                                                           <  SRR19973069.1732346/150‑1 (MQ=60)
                                             CCAGTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCT                                                                                                         <  SRR19973069.554366/150‑1 (MQ=60)
                                              CATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTT                                                                                                        <  SRR19973069.2445431/150‑1 (MQ=60)
                                              CATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTT                                                                                                        <  SRR19973069.2445876/150‑1 (MQ=60)
                                              CATTCGCTTCGATACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTT                                                                                                        <  SRR19973069.2600830/150‑1 (MQ=60)
                                              CATTCGCTTCGATACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAGTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTT                                                                                                        <  SRR19973069.2601234/150‑1 (MQ=60)
                                                   GCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGAGGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATG                                                                                                   <  SRR19973069.2286949/150‑1 (MQ=60)
                                                    CTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGA                                                                                                  >  SRR19973069.2493478/1‑150 (MQ=60)
                                                      TCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACA                                                                                                <  SRR19973069.1529224/150‑1 (MQ=60)
                                                           ACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCG                                                                                           <  SRR19973069.1249289/150‑1 (MQ=60)
                                                                TATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTT                                                                                      >  SRR19973069.3198050/1‑150 (MQ=60)
                                                                 ATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                     <  SRR19973069.1833175/150‑1 (MQ=60)
                                                                    CCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAT                                                                                  <  SRR19973069.3391482/150‑1 (MQ=60)
                                                                    CCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGAGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAT                                                                                  <  SRR19973069.838677/150‑1 (MQ=60)
                                                                      GCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCT                                                                                >  SRR19973069.1559150/1‑150 (MQ=60)
                                                                      GCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCT                                                                                <  SRR19973069.3705398/150‑1 (MQ=60)
                                                                       CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                               >  SRR19973069.3427082/1‑150 (MQ=60)
                                                                       CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                               >  SRR19973069.3427083/1‑150 (MQ=60)
                                                                       CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                               >  SRR19973069.3427093/1‑150 (MQ=60)
                                                                        AAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGC                                                                              >  SRR19973069.2620997/1‑150 (MQ=60)
                                                                        AAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGC                                                                              >  SRR19973069.214697/1‑150 (MQ=60)
                                                                         AATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCT                                                                             <  SRR19973069.2153092/150‑1 (MQ=60)
                                                                         AATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCT                                                                             >  SRR19973069.2611335/1‑150 (MQ=60)
                                                                          ATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGACTTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTG                                                                            >  SRR19973069.1794000/1‑150 (MQ=60)
                                                                          ATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTG                                                                            >  SRR19973069.3699346/1‑150 (MQ=60)
                                                                          ATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTG                                                                            <  SRR19973069.1783479/150‑1 (MQ=60)
                                                                           TCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGA                                                                           >  SRR19973069.798229/1‑150 (MQ=60)
                                                                            CTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAA                                                                          >  SRR19973069.1871096/1‑150 (MQ=60)
                                                                              TTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAG                                                                        <  SRR19973069.638068/150‑1 (MQ=60)
                                                                                AACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTG                                                                      <  SRR19973069.2341955/150‑1 (MQ=60)
                                                                                AACGATGAAGTGGTGGTGCCAGCGTTCCACAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTG                                                                      >  SRR19973069.2237999/1‑150 (MQ=60)
                                                                                  CGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAT                                                                    <  SRR19973069.2951571/150‑1 (MQ=60)
                                                                                  CGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAT                                                                    >  SRR19973069.1836675/1‑150 (MQ=60)
                                                                                  CGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAT                                                                    >  SRR19973069.3529700/1‑150 (MQ=60)
                                                                                     TGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA                                                                 >  SRR19973069.1982919/1‑150 (MQ=60)
                                                                                          TGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCT                                                            <  SRR19973069.1604807/150‑1 (MQ=60)
                                                                                          TGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCT                                                            <  SRR19973069.1603816/150‑1 (MQ=60)
                                                                                            GTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGC                                                          >  SRR19973069.3784681/1‑150 (MQ=60)
                                                                                              GGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTGCCGGGAGAAACTACGGATTTAATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                        <  SRR19973069.3770700/150‑1 (MQ=60)
                                                                                              GGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                        <  SRR19973069.2314754/150‑1 (MQ=60)
                                                                                              GGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                        <  SRR19973069.3771854/150‑1 (MQ=60)
                                                                                              GGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                        <  SRR19973069.2056504/150‑1 (MQ=60)
                                                                                               GTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTG                                                       <  SRR19973069.656576/150‑1 (MQ=60)
                                                                                                    AGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGA                                                  >  SRR19973069.65261/1‑150 (MQ=60)
                                                                                                         TCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATG                                             <  SRR19973069.2193032/150‑1 (MQ=60)
                                                                                                          CCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGA                                            >  SRR19973069.896409/1‑150 (MQ=60)
                                                                                                          CCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGA                                            >  SRR19973069.896751/1‑150 (MQ=60)
                                                                                                          CCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGA                                            <  SRR19973069.1887555/150‑1 (MQ=60)
                                                                                                           CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAA                                           >  SRR19973069.1159792/1‑150 (MQ=60)
                                                                                                              AACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGC                                        >  SRR19973069.225902/1‑150 (MQ=60)
                                                                                                                 CGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAT                                     <  SRR19973069.415301/150‑1 (MQ=60)
                                                                                                                 CGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAT                                     <  SRR19973069.415290/150‑1 (MQ=60)
                                                                                                                 CGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAT                                     <  SRR19973069.3336952/150‑1 (MQ=60)
                                                                                                                 CGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAT                                     <  SRR19973069.3337179/150‑1 (MQ=60)
                                                                                                                 CGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAT                                     <  SRR19973069.3337164/150‑1 (MQ=60)
                                                                                                                   TGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGC                                   <  SRR19973069.2009604/150‑1 (MQ=60)
                                                                                                                     GCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTG                                 <  SRR19973069.1662792/150‑1 (MQ=60)
                                                                                                                       GTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCG                               <  SRR19973069.1303077/150‑1 (MQ=60)
                                                                                                                            GCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGAC                          <  SRR19973069.841815/150‑1 (MQ=60)
                                                                                                                            GCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGAC                          <  SRR19973069.700374/150‑1 (MQ=60)
                                                                                                                             CTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACT                         <  SRR19973069.2006840/150‑1 (MQ=60)
                                                                                                                              TGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTT                        >  SRR19973069.15518/1‑150 (MQ=60)
                                                                                                                                GAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTAT                      <  SRR19973069.2980785/150‑1 (MQ=60)
                                                                                                                                  AGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGA                    >  SRR19973069.581555/1‑150 (MQ=60)
                                                                                                                                  AGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGA                    <  SRR19973069.710306/150‑1 (MQ=60)
                                                                                                                                       TGCGCGACTATTCCGTAGGGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTT               <  SRR19973069.3680217/150‑1 (MQ=60)
                                                                                                                                         CGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTA             >  SRR19973069.2526073/1‑150 (MQ=60)
                                                                                                                                           CGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACT           <  SRR19973069.2405569/150‑1 (MQ=60)
                                                                                                                                             ACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGG         >  SRR19973069.1861675/1‑150 (MQ=60)
                                                                                                                                              CTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGA        >  SRR19973069.2920130/1‑150 (MQ=60)
                                                                                                                                              CTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGA        >  SRR19973069.2919958/1‑150 (MQ=60)
                                                                                                                                              CTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGA        >  SRR19973069.1600776/1‑150 (MQ=60)
                                                                                                                                                ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGGAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC      >  SRR19973069.3388722/1‑150 (MQ=60)
                                                                                                                                                    CTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  >  SRR19973069.3451957/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCAGAT  >  CP009273/4135019‑4135316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑