Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F361 I0 R1	1353	250.7	8230166	96.0%	7900959	148.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,135,168	G→T	100%	V416L (GTA→TTA)	pflD →	putative glycine radical domain‑containing pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,135,168	0	G	T	97.3%	826.6 / 10.6	262	V416L (GTA→TTA)	pflD	putative glycine radical domain‑containing pyruvate formate‑lyase
Reads supporting (aligned to +/- strand): ref base G (5/2); new base T (96/159); total (101/161)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 262 total aligned reads displayed.

ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCA  >  CP009273/4135022‑4135313

aCGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTa                                                                                                                                                 >  2:1710232/1‑149 (MQ=255)

   ccTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTg                                                                                                                                              <  2:720424/149‑1 (MQ=255)

    cTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTgg                                                                                                                                             >  1:1514869/1‑149 (MQ=255)

     ttGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTggg                                                                                                                                            >  2:635581/1‑149 (MQ=255)

          cGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCtgtg                                                                                                                                       >  1:633256/1‑149 (MQ=255)

              acGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGa                                                                                                                                   >  1:3010088/1‑149 (MQ=255)

                gCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAt                                                                                                                                 >  1:1865973/1‑149 (MQ=255)

                gCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGGACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAt                                                                                                                                 <  1:3190235/149‑1 (MQ=255)

                    ttCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATc                                                                                                                             >  2:393028/1‑149 (MQ=255)

                      ccTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTa                                                                                                                           <  2:88425/149‑1 (MQ=255)

                       cTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAt                                                                                                                          <  1:1967261/149‑1 (MQ=255)

                        tgatgaAAACCGCCGAAACCAGTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAtt                                                                                                                         <  2:2810864/149‑1 (MQ=255)

                          atgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTcc                                                                                                                       >  1:2779897/1‑149 (MQ=255)

                          atgaAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTcc                                                                                                                       <  1:3761913/149‑1 (MQ=255)

                           tgaAAACCGCCGAAACCATTCGCTTTGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTccc                                                                                                                      <  1:2613260/149‑1 (MQ=255)

                                aCCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAg                                                                                                                 <  1:1044733/149‑1 (MQ=255)

                                     cgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                            >  1:366589/1‑149 (MQ=255)

                                     cgAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                            <  2:3263856/149‑1 (MQ=255)

                                     cgAAACCATTCGCGTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCt                                                                                                            <  2:2285804/149‑1 (MQ=255)

                                      gAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTa                                                                                                           <  1:2701579/149‑1 (MQ=255)

                                       aaaCCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAc                                                                                                          <  1:3355024/149‑1 (MQ=255)

                                         aCCATTCGCTTCGGTACCGGTATTCCTCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGAGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACgg                                                                                                        >  1:1468426/1‑149 (MQ=255)

                                           cATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCt                                                                                                      <  2:2255293/149‑1 (MQ=255)

                                            cttCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCtt                                                                                                     <  2:463885/148‑1 (MQ=255)

                                            aTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCtt                                                                                                     >  2:3903369/1‑149 (MQ=255)

                                               cGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCa                                                                                                  <  1:2027947/149‑1 (MQ=255)

                                                gcgtCGGGACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTAGTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 <  2:1433472/146‑1 (MQ=255)

                                                gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 <  1:2895549/149‑1 (MQ=255)

                                                gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 <  2:211375/149‑1 (MQ=255)

                                                gCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAt                                                                                                 >  2:1207302/1‑149 (MQ=255)

                                                 cTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATg                                                                                                <  1:3923600/149‑1 (MQ=255)

                                                 cTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGCGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATg                                                                                                >  1:3475587/1‑149 (MQ=255)

                                                  ttCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGa                                                                                               <  1:2469651/149‑1 (MQ=255)

                                                  ttCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGa                                                                                               <  1:2350828/149‑1 (MQ=255)

                                                    cGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACa                                                                                             <  2:2608064/149‑1 (MQ=255)

                                                     ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                            <  1:560781/149‑1 (MQ=255)

                                                     ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                            >  2:1813338/1‑149 (MQ=255)

                                                     ggTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAt                                                                                            >  2:1994370/1‑149 (MQ=255)

                                                      gTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTCTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATc                                                                                           >  1:3920549/1‑149 (MQ=255)

                                                        aCCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgc                                                                                         <  2:1647904/149‑1 (MQ=255)

                                                         ccGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATcgcg                                                                                        >  2:2421758/1‑149 (MQ=255)

                                                          cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGATGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATGGCGa                                                                                       <  2:686443/149‑1 (MQ=255)

                                                          cGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGa                                                                                       <  2:3627165/149‑1 (MQ=255)

                                                          cGGTAGTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGa                                                                                       <  1:3104860/149‑1 (MQ=255)

                                                            gTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATg                                                                                     >  2:92389/1‑149 (MQ=255)

                                                             tATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGTGCTGTGTGGAATTATCTATTACCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                    >  2:2925553/1‑149 (MQ=255)

                                                             tATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGt                                                                                    <  2:1538827/149‑1 (MQ=255)

                                                              aTTCCACAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGtt                                                                                   <  1:3938386/149‑1 (MQ=255)

                                                              aTTCCACAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGtt                                                                                   <  1:3077099/149‑1 (MQ=255)

                                                                  cGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                               <  2:1880170/149‑1 (MQ=255)

                                                                  cGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                               >  1:2073065/1‑149 (MQ=255)

                                                                  cGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAt                                                                               <  1:3627053/149‑1 (MQ=255)

                                                                     aaaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                            >  1:3671246/1‑149 (MQ=255)

                                                                     aaaTCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATctg                                                                            >  2:130626/1‑149 (MQ=255)

                                                                         cTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa                                                                        <  2:585060/149‑1 (MQ=255)

                                                                         cTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGa                                                                        <  1:175994/149‑1 (MQ=255)

                                                                          tttAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGaa                                                                       <  1:1939809/149‑1 (MQ=255)

                                                                             aaCGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGt                                                                    >  2:3724995/1‑149 (MQ=255)

                                                                             aaCGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGt                                                                    >  2:3728410/1‑149 (MQ=255)

                                                                               cGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGa                                                                  <  2:443631/149‑1 (MQ=255)

                                                                               cGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGa                                                                  <  1:3879783/149‑1 (MQ=255)

                                                                                gATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                 <  1:524205/149‑1 (MQ=255)

                                                                                gATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                 <  2:1975962/149‑1 (MQ=255)

                                                                                gATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAt                                                                 <  1:3329029/149‑1 (MQ=255)

                                                                                  tGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATgg                                                               >  2:1760510/1‑149 (MQ=255)

                                                                                    aaGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaa                                                             <  1:824215/149‑1 (MQ=255)

                                                                                     aGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCCCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGaaa                                                            <  2:961840/149‑1 (MQ=255)

                                                                                      gtggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                           <  1:1314164/149‑1 (MQ=255)

                                                                                      gtggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                           >  1:1250476/1‑149 (MQ=255)

                                                                                      gtggtggtgCCAGCGTTCCTCAACCGCGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAt                                                           <  2:2564816/149‑1 (MQ=255)

                                                                                       tggtggtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATc                                                          <  2:160245/149‑1 (MQ=255)

                                                                                            gtgCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATctgcct                                                     <  1:1584531/149‑1 (MQ=255)

                                                                                              gCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGCCATCGCGATGTTTAATCTGCTGAAAGTGATGGCAATctgcctgc                                                   >  1:2148262/1‑149 (MQ=255)

                                                                                                cAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCAt                                                 <  1:960580/149‑1 (MQ=255)

                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGa                                               >  2:2486816/1‑149 (MQ=255)

                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGa                                               >  2:2339235/1‑149 (MQ=255)

                                                                                                  gCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGa                                               >  1:2251145/1‑149 (MQ=255)

                                                                                                    gTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGaaa                                             <  1:2863431/149‑1 (MQ=255)

                                                                                                        cTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGa                                         >  1:484619/1‑149 (MQ=255)

                                                                                                             ccGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                    <  2:1179326/149‑1 (MQ=255)

                                                                                                             ccGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCa                                    <  1:267393/149‑1 (MQ=255)

                                                                                                               gTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAt                                  >  2:3687240/1‑149 (MQ=255)

                                                                                                                   cGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtgctg                              <  1:4025309/149‑1 (MQ=255)

                                                                                                                    gTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAAtgctgc                             <  1:854295/149‑1 (MQ=255)

                                                                                                                      ttCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTTTCTCGTCACTTCATAACCTAGGGCTTGCATTACATCTCGATGTTTACTCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTgcgc                           <  1:3555522/149‑1 (MQ=255)

                                                                                                                            ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACtt                     >  1:1032318/1‑149 (MQ=255)

                                                                                                                            ggAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACtt                     <  1:2339235/149‑1 (MQ=255)

                                                                                                                                gATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGa                 >  2:3952054/1‑149 (MQ=255)

                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  2:2336004/1‑149 (MQ=255)

                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  2:3011268/1‑149 (MQ=255)

                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  2:1071394/1‑149 (MQ=255)

                                                                                                                                  tGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               <  2:2512426/149‑1 (MQ=255)

                                                                                                                                  tGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAg               >  1:4082283/1‑149 (MQ=255)

                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  1:3498702/1‑149 (MQ=255)

                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  2:313827/1‑149 (MQ=255)

                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  2:1387547/1‑149 (MQ=255)

                                                                                                                                          aCTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGAGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTg       >  2:3046186/1‑149 (MQ=255)

                                                                                                                                            tATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGa     <  2:3475907/149‑1 (MQ=255)

                                                                                                                                             aTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAg    <  2:3323918/149‑1 (MQ=255)

                                                                                                                                              ttCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGc   >  2:2924292/1‑149 (MQ=255)

ACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGCA  >  CP009273/4135022‑4135313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  CP009273/4135019‑4135312

CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCT                                                                                                                                                  >  SRR19973068.1573616/1‑150 (MQ=60)

CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCT                                                                                                                                                  >  SRR19973068.129635/1‑150 (MQ=60)

 TAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTT                                                                                                                                                 <  SRR19973068.3480198/150‑1 (MQ=60)

      CCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGG                                                                                                                                            <  SRR19973068.2616965/150‑1 (MQ=60)

      CCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGG                                                                                                                                            >  SRR19973068.1524835/1‑150 (MQ=60)

      CCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGG                                                                                                                                            <  SRR19973068.2621139/150‑1 (MQ=60)

          GATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTG                                                                                                                                        >  SRR19973068.1933973/1‑150 (MQ=60)

            TCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTG                                                                                                                                      >  SRR19973068.2714041/1‑150 (MQ=60)

            TCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTG                                                                                                                                      >  SRR19973068.637430/1‑150 (MQ=60)

             CGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCATTAGTGGGCTGTGT                                                                                                                                     >  SRR19973068.1824369/1‑150 (MQ=60)

               ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGG                                                                                                                                   >  SRR19973068.1280639/1‑150 (MQ=60)

               ACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGG                                                                                                                                   <  SRR19973068.1993234/150‑1 (MQ=60)

                CACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGA                                                                                                                                  >  SRR19973068.3028647/1‑150 (MQ=60)

                  CGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAAT                                                                                                                                >  SRR19973068.1878313/1‑150 (MQ=60)

                   GCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGGACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATT                                                                                                                               <  SRR19973068.3209874/150‑1 (MQ=60)

                   GCCGTTACTGATGAAAACCGCCGAATCCTTTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGAGTTTCGCTGGTAGATGCGCGCGAATATTCCTTAGTGGGCTGTGTGGAATT                                                                                                                               <  SRR19973068.35345/150‑1 (MQ=60)

                    CCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTA                                                                                                                              <  SRR19973068.2742111/150‑1 (MQ=60)

                    CCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTA                                                                                                                              >  SRR19973068.42840/1‑150 (MQ=60)

                          CTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATT                                                                                                                        <  SRR19973068.1980303/150‑1 (MQ=60)

                            GATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCC                                                                                                                      >  SRR19973068.2797086/1‑150 (MQ=60)

                             ATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCC                                                                                                                     <  SRR19973068.3784386/150‑1 (MQ=60)

                             ATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCC                                                                                                                     <  SRR19973068.2253185/150‑1 (MQ=60)

                              TGAAAACCGCCGAAACCATTCGCTTTGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCG                                                                                                                    <  SRR19973068.2629725/150‑1 (MQ=60)

                              TGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCG                                                                                                                    <  SRR19973068.512157/150‑1 (MQ=60)

                                  AACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTTGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG                                                                                                                >  SRR19973068.3273065/1‑150 (MQ=60)

                                   ACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGA                                                                                                               <  SRR19973068.1051451/150‑1 (MQ=60)

                                       CCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCT                                                                                                           >  SRR19973068.369050/1‑150 (MQ=60)

                                       CCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCT                                                                                                           >  SRR19973068.369041/1‑150 (MQ=60)

                                         GAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAC                                                                                                         <  SRR19973068.55463/150‑1 (MQ=60)

                                         GAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTAC                                                                                                         <  SRR19973068.2718349/150‑1 (MQ=60)

                                          AAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACG                                                                                                        <  SRR19973068.3375450/150‑1 (MQ=60)

                                           AACCATTCGCTTCGGTACCGGTATTCCTCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGAGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGG                                                                                                       >  SRR19973068.1478075/1‑150 (MQ=60)

                                           AACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGG                                                                                                       >  SRR19973068.1478104/1‑150 (MQ=60)

                                               ATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTG                                                                                                   >  SRR19973068.2069751/1‑150 (MQ=60)

                                                  CGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAT                                                                                                <  SRR19973068.2041236/150‑1 (MQ=60)

                                                  CGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCAT                                                                                                <  SRR19973068.1159926/150‑1 (MQ=60)

                                                   GCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATG                                                                                               <  SRR19973068.2913528/150‑1 (MQ=60)

                                                   GCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATG                                                                                               <  SRR19973068.2913574/150‑1 (MQ=60)

                                                   GCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATG                                                                                               >  SRR19973068.2962621/1‑150 (MQ=60)

                                                   GCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGCGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATG                                                                                               >  SRR19973068.3496674/1‑150 (MQ=60)

                                                    CTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGA                                                                                              >  SRR19973068.3982221/1‑150 (MQ=60)

                                                    CTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGA                                                                                              <  SRR19973068.3945905/150‑1 (MQ=60)

                                                    CTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGA                                                                                              <  SRR19973068.3947359/150‑1 (MQ=60)

                                                    CTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAGTCCCGGCAGAACCTACGGCTTGCATGA                                                                                              <  SRR19973068.3945967/150‑1 (MQ=60)

                                                     TTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGAC                                                                                             <  SRR19973068.2365900/150‑1 (MQ=60)

                                                     TTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGAC                                                                                             <  SRR19973068.2485414/150‑1 (MQ=60)

                                                     TTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGAC                                                                                             <  SRR19973068.155687/150‑1 (MQ=60)

                                                      TCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACA                                                                                            <  SRR19973068.3563747/150‑1 (MQ=60)

                                                       CGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACAT                                                                                           <  SRR19973068.3415573/150‑1 (MQ=60)

                                                        GGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCGACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATC                                                                                          >  SRR19973068.2135480/1‑150 (MQ=60)

                                                        GGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTCTTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATC                                                                                          >  SRR19973068.3944285/1‑150 (MQ=60)

                                                        GGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATC                                                                                          <  SRR19973068.564644/150‑1 (MQ=60)

                                                           ACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTAGTCCCGGCAGAACCTACGGCTTGCATGACATCGCG                                                                                       <  SRR19973068.3442658/150‑1 (MQ=60)

                                                           ACCGGGATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCG                                                                                       <  SRR19973068.2892184/150‑1 (MQ=60)

                                                             CGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGAT                                                                                     <  SRR19973068.3123750/150‑1 (MQ=60)

                                                             CGGTAGTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGAT                                                                                     <  SRR19973068.3123780/150‑1 (MQ=60)

                                                              GGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATG                                                                                    <  SRR19973068.618307/150‑1 (MQ=60)

                                                              GGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATG                                                                                    <  SRR19973068.618366/150‑1 (MQ=60)

                                                                 ATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                 <  SRR19973068.2074857/150‑1 (MQ=60)

                                                                 ATTCCACAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                 <  SRR19973068.3962232/150‑1 (MQ=60)

                                                                 ATTCCACAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTT                                                                                 <  SRR19973068.3095870/150‑1 (MQ=60)

                                                                  TTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTA                                                                                >  SRR19973068.3973680/1‑150 (MQ=60)

                                                                    CCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAAT                                                                              >  SRR19973068.2086499/1‑150 (MQ=60)

                                                                     CGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATC                                                                             <  SRR19973068.3649051/150‑1 (MQ=60)

                                                                     CGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATC                                                                             >  SRR19973068.2403584/1‑150 (MQ=60)

                                                                     CGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATC                                                                             >  SRR19973068.317534/1‑150 (MQ=60)

                                                                     CGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATC                                                                             <  SRR19973068.3059961/150‑1 (MQ=60)

                                                                     CGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATC                                                                             <  SRR19973068.3060372/150‑1 (MQ=60)

                                                                     CGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATC                                                                             <  SRR19973068.3649040/150‑1 (MQ=60)

                                                                      GCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCT                                                                            <  SRR19973068.1387117/150‑1 (MQ=60)

                                                                      GCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCT                                                                            <  SRR19973068.395761/150‑1 (MQ=60)

                                                                       CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                           >  SRR19973068.3693434/1‑150 (MQ=60)

                                                                       CAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTG                                                                           >  SRR19973068.407010/1‑150 (MQ=60)

                                                                            CTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAA                                                                      >  SRR19973068.1972587/1‑150 (MQ=60)

                                                                            CTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAA                                                                      <  SRR19973068.177412/150‑1 (MQ=60)

                                                                             TTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAA                                                                     <  SRR19973068.1953217/150‑1 (MQ=60)

                                                                             TTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAA                                                                     <  SRR19973068.1952703/150‑1 (MQ=60)

                                                                             TTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAA                                                                     <  SRR19973068.1952701/150‑1 (MQ=60)

                                                                              TTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAG                                                                    <  SRR19973068.337509/150‑1 (MQ=60)

                                                                              TTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAG                                                                    <  SRR19973068.3614260/150‑1 (MQ=60)

                                                                                 ACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGA                                                                 <  SRR19973068.823914/150‑1 (MQ=60)

                                                                                  CGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGAT                                                                <  SRR19973068.3903217/150‑1 (MQ=60)

                                                                                  CGATGAAGTGGTGGTGCCAGCGTTCCTAATCCGTGGCGTTTAGCTGGATGATGCGCGCGACTATTACTTAGTTGGCTGTGTGGAATTATCTATTCCAGGCAGAAGCTTCGGCTTGCTTGACATCGCGTTGTTTAATCTGCTGAAAGTGAT                                                                <  SRR19973068.3903102/150‑1 (MQ=60)

                                                                                   GATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATG                                                               <  SRR19973068.3349351/150‑1 (MQ=60)

                                                                                   GATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATG                                                               <  SRR19973068.2867320/150‑1 (MQ=60)

                                                                                   GATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATG                                                               <  SRR19973068.2372206/150‑1 (MQ=60)

                                                                                   GATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATG                                                               <  SRR19973068.527825/150‑1 (MQ=60)

                                                                                    ATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGG                                                              <  SRR19973068.3914867/150‑1 (MQ=60)

                                                                                     TGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGA                                                             <  SRR19973068.3935480/150‑1 (MQ=60)

                                                                                      GAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAA                                                            <  SRR19973068.3736409/150‑1 (MQ=60)

                                                                                       AAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAA                                                           <  SRR19973068.829859/150‑1 (MQ=60)

                                                                                       AAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAA                                                           <  SRR19973068.829815/150‑1 (MQ=60)

                                                                                        AGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAAT                                                          >  SRR19973068.1258745/1‑150 (MQ=60)

                                                                                         GTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATC                                                         <  SRR19973068.1322753/150‑1 (MQ=60)

                                                                                              GGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                    <  SRR19973068.2747286/150‑1 (MQ=60)

                                                                                              GGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                    <  SRR19973068.2713852/150‑1 (MQ=60)

                                                                                              GGTGCCAGCGGTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCT                                                    <  SRR19973068.3099144/150‑1 (MQ=60)

                                                                                               GTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTG                                                   <  SRR19973068.1594940/150‑1 (MQ=60)

                                                                                                TGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGCCATCGCGATGTTTAATCTGCTGAAAGTGATGGCAATCTGCCTGC                                                  >  SRR19973068.2162331/1‑150 (MQ=60)

                                                                                                   CAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATG                                               <  SRR19973068.966975/150‑1 (MQ=60)

                                                                                                    AGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGA                                              >  SRR19973068.2265799/1‑150 (MQ=60)

                                                                                                       GTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAA                                           <  SRR19973068.2881269/150‑1 (MQ=60)

                                                                                                       GTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAA                                           >  SRR19973068.1727678/1‑150 (MQ=60)

                                                                                                        TTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAAT                                          <  SRR19973068.1045391/150‑1 (MQ=60)

                                                                                                          CCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGA                                        >  SRR19973068.488023/1‑150 (MQ=60)

                                                                                                           CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAA                                       <  SRR19973068.1150566/150‑1 (MQ=60)

                                                                                                           CTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAA                                       <  SRR19973068.503868/150‑1 (MQ=60)

                                                                                                              AACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGC                                    <  SRR19973068.4092991/150‑1 (MQ=60)

                                                                                                                CCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAA                                  <  SRR19973068.269278/150‑1 (MQ=60)

                                                                                                                CCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAA                                  <  SRR19973068.2355651/150‑1 (MQ=60)

                                                                                                                   TGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGC                               >  SRR19973068.3659559/1‑150 (MQ=60)

                                                                                                                   TGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGC                               >  SRR19973068.3659424/1‑150 (MQ=60)

                                                                                                                    GGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCT                              <  SRR19973068.3512027/150‑1 (MQ=60)

                                                                                                                    GGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCT                              <  SRR19973068.2311682/150‑1 (MQ=60)

                                                                                                                    GGCGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCT                              <  SRR19973068.2311663/150‑1 (MQ=60)

                                                                                                                      CGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGC                            <  SRR19973068.4049593/150‑1 (MQ=60)

                                                                                                                      CGTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGC                            <  SRR19973068.1693607/150‑1 (MQ=60)

                                                                                                                       GTTTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCG                           <  SRR19973068.860103/150‑1 (MQ=60)

                                                                                                                         TTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTTTCTCGTCACTTCATAACCTAGGGCTTGCATTACATCTCGATGTTTACTCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCT                         <  SRR19973068.3577120/150‑1 (MQ=60)

                                                                                                                         TTCGCTGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCT                         <  SRR19973068.3303106/150‑1 (MQ=60)

                                                                                                                              TGGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTT                    >  SRR19973068.1038965/1‑150 (MQ=60)

                                                                                                                               GGAAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTA                   <  SRR19973068.2354265/150‑1 (MQ=60)

                                                                                                                                 AAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATG                 <  SRR19973068.947622/150‑1 (MQ=60)

                                                                                                                                 AAGATGCGCGCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATG                 >  SRR19973068.953670/1‑150 (MQ=60)

                                                                                                                                    ATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAG              >  SRR19973068.4106772/1‑150 (MQ=60)

                                                                                                                                          GCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTAC        <  SRR19973068.1510933/150‑1 (MQ=60)

                                                                                                                                          GCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTAC        <  SRR19973068.698522/150‑1 (MQ=60)

                                                                                                                                          GCGACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGNTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTAC        <  SRR19973068.2098751/150‑1 (MQ=60)

                                                                                                                                            GACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTG      >  SRR19973068.3519957/1‑150 (MQ=60)

                                                                                                                                            GACTATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGATGACTTATGAAGGTTTACTG      >  SRR19973068.2635084/1‑150 (MQ=60)

                                                                                                                                               TATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAG   >  SRR19973068.3697981/1‑150 (MQ=60)

                                                                                                                                                ATTCCTTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  SRR19973068.134297/1‑150 (MQ=60)

CTAACGCCTTGATCGACACGCCGTTCCTGATGAAAACCGCCGAAACCATTCGCTTCGGTACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCGTTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAGAACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATGCTGCGCTGACTTATGAAGGTTTACTGGAGC  >  CP009273/4135019‑4135312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`