Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 581,633 G→T 72.5% L244L (CTC→CTA)  envY ← porin thermoregulatory transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273581,6330GT72.5% 338.8 / 215.0 258L244L (CTC→CTA) envYporin thermoregulatory transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (36/35);  new base T (90/96);  total (126/132)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTC  >  CP009273/581486‑581779
                                                                                                                                                   |                                                                                                                                                  
tgttgACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCtag                                                                                                                                                  >  2:1892275/1‑150 (MQ=255)
 gttgACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCTCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCgaga                                                                                                                                                 >  2:943920/1‑150 (MQ=255)
 gttgACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCgaga                                                                                                                                                 <  2:1541796/150‑1 (MQ=255)
   tgACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATa                                                                                                                                               <  1:4372217/150‑1 (MQ=255)
   tgACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATa                                                                                                                                               <  2:2714522/150‑1 (MQ=255)
       ttAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTc                                                                                                                                           <  1:2764223/150‑1 (MQ=255)
         aaaaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCaa                                                                                                                                         >  2:3949506/1‑150 (MQ=255)
           aaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACg                                                                                                                                       <  1:2251757/150‑1 (MQ=255)
            aTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACgg                                                                                                                                      >  1:4529559/1‑150 (MQ=255)
            aTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACgg                                                                                                                                      <  2:1575053/150‑1 (MQ=255)
             tGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGgt                                                                                                                                     >  1:3155320/1‑150 (MQ=255)
             tGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgt                                                                                                                                     <  2:4348443/150‑1 (MQ=255)
                ctttCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTc                                                                                                                                  <  1:2312042/148‑1 (MQ=255)
                 tttCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCa                                                                                                                                 >  1:636983/2‑150 (MQ=255)
                     tAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGcc                                                                                                                             >  2:4359981/1‑150 (MQ=255)
                     tAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGcc                                                                                                                             <  1:894349/150‑1 (MQ=255)
                        gAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTa                                                                                                                          >  1:953582/1‑150 (MQ=255)
                        gAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTa                                                                                                                          <  1:4553657/150‑1 (MQ=255)
                        gAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTa                                                                                                                          <  1:1163715/150‑1 (MQ=255)
                        gAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCGTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTa                                                                                                                          <  1:2725227/150‑1 (MQ=255)
                           cTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTaaaa                                                                                                                       >  2:189445/1‑150 (MQ=255)
                           cTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTaaaa                                                                                                                       >  2:129462/1‑150 (MQ=255)
                             tCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACg                                                                                                                     <  2:2369280/150‑1 (MQ=255)
                               tAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGcc                                                                                                                   >  1:2157413/1‑150 (MQ=255)
                               tAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGcc                                                                                                                   <  1:659848/150‑1 (MQ=255)
                                 aGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCtt                                                                                                                 <  1:3339061/150‑1 (MQ=255)
                                 aGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCtt                                                                                                                 >  1:4168531/1‑150 (MQ=255)
                                  gTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTa                                                                                                                <  1:4653447/150‑1 (MQ=255)
                                    cGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaa                                                                                                              <  2:2561037/150‑1 (MQ=255)
                                    cGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaa                                                                                                              <  1:3690942/150‑1 (MQ=255)
                                      tGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaaaa                                                                                                            >  1:4729468/1‑150 (MQ=255)
                                       gggCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCTGGCCGTAAAACGCCTTAAAAAc                                                                                                           >  2:1945239/1‑150 (MQ=255)
                                           cGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACagag                                                                                                       >  2:564586/1‑150 (MQ=255)
                                           cGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACagag                                                                                                       >  1:2421122/1‑150 (MQ=255)
                                               ggTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATaa                                                                                                   >  1:1735067/1‑150 (MQ=255)
                                                gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCTCTTTGCCCGTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATaaa                                                                                                  <  2:2004795/150‑1 (MQ=255)
                                                    gAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTac                                                                                              <  2:826107/150‑1 (MQ=255)
                                                     aaTCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTCGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTacg                                                                                             >  2:1856260/1‑150 (MQ=255)
                                                      aTCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTacga                                                                                            >  1:2448143/1‑150 (MQ=255)
                                                             aGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                     <  2:5127094/150‑1 (MQ=255)
                                                             aGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                     <  2:3645955/150‑1 (MQ=255)
                                                             aGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                     >  2:2589104/1‑150 (MQ=255)
                                                             aGGGCGCGCCATTTCGTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                     <  2:2509722/150‑1 (MQ=255)
                                                                gcgcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCta                                                                                  <  1:126269/150‑1 (MQ=255)
                                                                 cgcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCtat                                                                                 >  1:995197/1‑150 (MQ=255)
                                                                 cgcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCtat                                                                                 <  1:2563604/150‑1 (MQ=255)
                                                                 cgcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCtat                                                                                 >  1:4030575/1‑150 (MQ=255)
                                                                  gcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCtata                                                                                <  2:755489/150‑1 (MQ=255)
                                                                  gcgcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCtata                                                                                >  1:4350278/1‑150 (MQ=255)
                                                                       aTTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCcac                                                                           <  2:4550032/150‑1 (MQ=255)
                                                                          tcttcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATa                                                                        <  2:2233087/150‑1 (MQ=255)
                                                                               cTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                   <  1:672511/150‑1 (MQ=255)
                                                                               cTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                   >  1:4969168/1‑150 (MQ=255)
                                                                                       tGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTg                                                           <  1:1639039/150‑1 (MQ=255)
                                                                                       tGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTg                                                           <  2:2904003/150‑1 (MQ=255)
                                                                                       tGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTg                                                           <  1:5020492/150‑1 (MQ=255)
                                                                                        gCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGa                                                          <  2:1040080/150‑1 (MQ=255)
                                                                                        gCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGa                                                          <  2:2198914/150‑1 (MQ=255)
                                                                                          ccGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGt                                                        <  2:3911427/150‑1 (MQ=255)
                                                                                          ccGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGt                                                        <  1:4878401/150‑1 (MQ=255)
                                                                                          ccGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGt                                                        <  1:3595580/150‑1 (MQ=255)
                                                                                           cGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTg                                                       >  2:3340418/1‑150 (MQ=255)
                                                                                            gTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGa                                                      >  2:2774234/1‑150 (MQ=255)
                                                                                            gTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGa                                                      <  2:4133167/150‑1 (MQ=255)
                                                                                             tCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGat                                                     <  1:1664782/150‑1 (MQ=255)
                                                                                                tATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAtt                                                  <  2:2599033/150‑1 (MQ=255)
                                                                                                 aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttt                                                 <  2:1495018/150‑1 (MQ=255)
                                                                                                 aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttt                                                 >  2:3229597/1‑150 (MQ=255)
                                                                                                 aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttt                                                 >  2:5061473/1‑150 (MQ=255)
                                                                                                  tCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAtttt                                                >  1:1045150/1‑150 (MQ=255)
                                                                                                  tCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAtttt                                                >  1:4035345/1‑150 (MQ=255)
                                                                                                   ccGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAttttt                                               >  2:3823515/1‑150 (MQ=255)
                                                                                                    cGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTg                                              >  2:3042396/1‑150 (MQ=255)
                                                                                                    cGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTg                                              >  1:597514/1‑150 (MQ=255)
                                                                                                     gTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGt                                             <  1:2073392/150‑1 (MQ=255)
                                                                                                         ccGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATc                                         >  2:4642538/1‑150 (MQ=255)
                                                                                                           gCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCa                                       >  2:4207579/1‑150 (MQ=255)
                                                                                                           gCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCa                                       >  1:3039167/1‑150 (MQ=255)
                                                                                                           gCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCa                                       <  2:1738721/150‑1 (MQ=255)
                                                                                                            cTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGATAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAATCAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGAGCCACCTGAGTGATATTTTTGTTATCCAt                                      >  1:2413738/1‑150 (MQ=255)
                                                                                                             tttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATc                                     >  1:4718482/1‑150 (MQ=255)
                                                                                                              ttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCa                                    <  2:4729143/150‑1 (MQ=255)
                                                                                                              ttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCa                                    <  2:15538/150‑1 (MQ=255)
                                                                                                                      caccacATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATc                            <  2:4756648/150‑1 (MQ=255)
                                                                                                                         cacaTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGt                         >  1:3263501/1‑150 (MQ=255)
                                                                                                                           caTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAGACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTAc                       >  2:3333238/1‑150 (MQ=255)
                                                                                                                           caTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTAc                       >  2:436948/1‑150 (MQ=255)
                                                                                                                           caTCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTAc                       <  1:350541/150‑1 (MQ=255)
                                                                                                                                 tttttGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATCGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTa                 <  2:2362757/150‑1 (MQ=255)
                                                                                                                                 tttttGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAAGTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTa                 <  2:2421122/150‑1 (MQ=255)
                                                                                                                                 ttgttGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTa                 <  2:212625/147‑1 (MQ=255)
                                                                                                                                  ttttGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTaa                >  1:1027931/1‑150 (MQ=255)
                                                                                                                                   tttGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAAc               <  1:1443651/150‑1 (MQ=255)
                                                                                                                                    ttGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACg              <  2:3468056/150‑1 (MQ=255)
                                                                                                                                    ttGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACg              <  1:2833375/150‑1 (MQ=255)
                                                                                                                                       tCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAt           >  1:1774665/1‑150 (MQ=255)
                                                                                                                                        cGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATa          <  2:4611768/150‑1 (MQ=255)
                                                                                                                                         gCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATAc         <  1:378750/150‑1 (MQ=255)
                                                                                                                                          cTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACg        >  2:4684016/1‑150 (MQ=255)
                                                                                                                                          cTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACg        <  2:2988403/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTC  >  CP009273/581486‑581779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGT  >  CP009273/581485‑581782
                                                                                                                                                    |                                                                                                                                                     
TTGTTGACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTA                                                                                                                                                      >  SRR19973063.2207729/1‑150 (MQ=60)
    TGACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATA                                                                                                                                                  <  SRR19973063.4386147/150‑1 (MQ=60)
    TGACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATA                                                                                                                                                  <  SRR19973063.4386209/150‑1 (MQ=60)
    TGACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATA                                                                                                                                                  <  SRR19973063.46138/150‑1 (MQ=60)
     GACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAA                                                                                                                                                 <  SRR19973063.5012214/150‑1 (MQ=60)
        TTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTC                                                                                                                                              <  SRR19973063.2773134/150‑1 (MQ=60)
           AAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAAC                                                                                                                                           <  SRR19973063.672066/150‑1 (MQ=60)
            AATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACG                                                                                                                                          <  SRR19973063.329985/150‑1 (MQ=60)
            AATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACG                                                                                                                                          <  SRR19973063.2258922/150‑1 (MQ=60)
            AATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACG                                                                                                                                          <  SRR19973063.1690173/150‑1 (MQ=60)
             ATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGG                                                                                                                                         >  SRR19973063.4544087/1‑150 (MQ=60)
              TGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGT                                                                                                                                        >  SRR19973063.3165466/1‑150 (MQ=60)
               GTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTG                                                                                                                                       <  SRR19973063.2771260/150‑1 (MQ=60)
                 CTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTC                                                                                                                                     <  SRR19973063.2319435/150‑1 (MQ=60)
                  TTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCA                                                                                                                                    >  SRR19973063.638859/1‑150 (MQ=60)
                  TTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCA                                                                                                                                    <  SRR19973063.959778/150‑1 (MQ=60)
                     CTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCCGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGC                                                                                                                                 >  SRR19973063.4232338/1‑150 (MQ=60)
                     CTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGC                                                                                                                                 >  SRR19973063.315365/1‑150 (MQ=60)
                      TAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCC                                                                                                                                <  SRR19973063.896949/150‑1 (MQ=60)
                      TAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCC                                                                                                                                <  SRR19973063.897237/150‑1 (MQ=60)
                       AGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCG                                                                                                                               <  SRR19973063.3627863/150‑1 (MQ=60)
                        TGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGT                                                                                                                              >  SRR19973063.3860691/1‑150 (MQ=60)
                        GGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGT                                                                                                                              <  SRR19973063.1484126/150‑1 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             <  SRR19973063.4568247/150‑1 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             >  SRR19973063.956557/1‑150 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             >  SRR19973063.956446/1‑150 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             <  SRR19973063.1167227/150‑1 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             <  SRR19973063.1060057/150‑1 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             <  SRR19973063.1167010/150‑1 (MQ=60)
                         GAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCGTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTA                                                                                                                             <  SRR19973063.2734026/150‑1 (MQ=60)
                             TTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAAC                                                                                                                         >  SRR19973063.4467269/1‑150 (MQ=60)
                             TTCTAAGTCGAGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAAC                                                                                                                         >  SRR19973063.2229058/1‑150 (MQ=60)
                                TAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCC                                                                                                                      >  SRR19973063.2164172/1‑150 (MQ=60)
                                TAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCC                                                                                                                      <  SRR19973063.661779/150‑1 (MQ=60)
                                  AGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTT                                                                                                                    <  SRR19973063.3349708/150‑1 (MQ=60)
                                  AGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTT                                                                                                                    >  SRR19973063.4181856/1‑150 (MQ=60)
                                   GTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTA                                                                                                                   <  SRR19973063.4668303/150‑1 (MQ=60)
                                     CGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAA                                                                                                                 >  SRR19973063.2111612/1‑150 (MQ=60)
                                     CGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAA                                                                                                                 <  SRR19973063.3702760/150‑1 (MQ=60)
                                     CGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAA                                                                                                                 <  SRR19973063.3702759/150‑1 (MQ=60)
                                      GTGGGCCGCAGGCTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAA                                                                                                                <  SRR19973063.2345862/150‑1 (MQ=60)
                                       TGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAA                                                                                                               >  SRR19973063.4744541/1‑150 (MQ=60)
                                            CGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAG                                                                                                          >  SRR19973063.4885923/1‑150 (MQ=60)
                                            CGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAG                                                                                                          >  SRR19973063.2428878/1‑150 (MQ=60)
                                            CGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAG                                                                                                          >  SRR19973063.2428864/1‑150 (MQ=60)
                                            CGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAG                                                                                                          >  SRR19973063.2428860/1‑150 (MQ=60)
                                               AGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATA                                                                                                       >  SRR19973063.4447739/1‑150 (MQ=60)
                                                GGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAA                                                                                                      >  SRR19973063.1740348/1‑150 (MQ=60)
                                                   TCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGT                                                                                                   >  SRR19973063.4567459/1‑150 (MQ=60)
                                                     GAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTAC                                                                                                 >  SRR19973063.3120719/1‑150 (MQ=60)
                                                       ATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGA                                                                                               >  SRR19973063.2455958/1‑150 (MQ=60)
                                                       ATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGA                                                                                               >  SRR19973063.2455818/1‑150 (MQ=60)
                                                         CCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACG                                                                                             <  SRR19973063.2385766/150‑1 (MQ=60)
                                                             CAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCT                                                                                         >  SRR19973063.389388/1‑150 (MQ=60)
                                                               GGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGC                                                                                       <  SRR19973063.668031/150‑1 (MQ=60)
                                                                 GCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTA                                                                                     <  SRR19973063.126678/150‑1 (MQ=60)
                                                                  CGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTAT                                                                                    >  SRR19973063.998200/1‑150 (MQ=60)
                                                                  CGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTAT                                                                                    <  SRR19973063.2571770/150‑1 (MQ=60)
                                                                  CGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTAT                                                                                    >  SRR19973063.4043495/1‑150 (MQ=60)
                                                                  CGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTAT                                                                                    >  SRR19973063.4043632/1‑150 (MQ=60)
                                                                   GCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATA                                                                                   >  SRR19973063.4364176/1‑150 (MQ=60)
                                                                       CATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCA                                                                               >  SRR19973063.2663578/1‑150 (MQ=60)
                                                                          TTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCCAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACAT                                                                            <  SRR19973063.584091/150‑1 (MQ=60)
                                                                             TTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAAT                                                                         >  SRR19973063.4129346/1‑150 (MQ=60)
                                                                             TTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAAT                                                                         >  SRR19973063.1621376/1‑150 (MQ=60)
                                                                              TCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATT                                                                        <  SRR19973063.290398/150‑1 (MQ=60)
                                                                               CCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTG                                                                       >  SRR19973063.1319058/1‑150 (MQ=60)
                                                                                CTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGCGATAAAGTACGACGTGCTGCTATAGCCACATAATTGC                                                                      <  SRR19973063.3460898/150‑1 (MQ=60)
                                                                                CTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGC                                                                      <  SRR19973063.674476/150‑1 (MQ=60)
                                                                                CTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGC                                                                      >  SRR19973063.4985225/1‑150 (MQ=60)
                                                                                 TCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCG                                                                     <  SRR19973063.4755723/150‑1 (MQ=60)
                                                                                 TCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCG                                                                     <  SRR19973063.825356/150‑1 (MQ=60)
                                                                                    TTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCA                                                                  <  SRR19973063.3217584/150‑1 (MQ=60)
                                                                                     TTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCAC                                                                 >  SRR19973063.4944028/1‑150 (MQ=60)
                                                                                        TGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTG                                                              >  SRR19973063.2161109/1‑150 (MQ=60)
                                                                                        TGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTG                                                              <  SRR19973063.5036691/150‑1 (MQ=60)
                                                                                        TGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTG                                                              <  SRR19973063.1644051/150‑1 (MQ=60)
                                                                                           CCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGT                                                           <  SRR19973063.4894131/150‑1 (MQ=60)
                                                                                           CCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGT                                                           <  SRR19973063.3607111/150‑1 (MQ=60)
                                                                                           CCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGT                                                           <  SRR19973063.3304186/150‑1 (MQ=60)
                                                                                              TCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGAT                                                        <  SRR19973063.1669859/150‑1 (MQ=60)
                                                                                                  ATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTT                                                    >  SRR19973063.2809055/1‑150 (MQ=60)
                                                                                                   TCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTT                                                   >  SRR19973063.1048315/1‑150 (MQ=60)
                                                                                                   TCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTT                                                   >  SRR19973063.4048275/1‑150 (MQ=60)
                                                                                                    CCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTT                                                  <  SRR19973063.287702/150‑1 (MQ=60)
                                                                                                     CGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTG                                                 >  SRR19973063.599275/1‑150 (MQ=60)
                                                                                                     CGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTG                                                 >  SRR19973063.599337/1‑150 (MQ=60)
                                                                                                      GTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGT                                                <  SRR19973063.2079875/150‑1 (MQ=60)
                                                                                                      GTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGT                                                <  SRR19973063.1847086/150‑1 (MQ=60)
                                                                                                         TCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTAT                                             >  SRR19973063.1411305/1‑150 (MQ=60)
                                                                                                            GCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCA                                          >  SRR19973063.3048877/1‑150 (MQ=60)
                                                                                                             CTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCAT                                         >  SRR19973063.4941096/1‑150 (MQ=60)
                                                                                                             CTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGATAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAATCAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGAGCCACCTGAGTGATATTTTTGTTATCCAT                                         >  SRR19973063.2421460/1‑150 (MQ=60)
                                                                                                              TTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATC                                        >  SRR19973063.4732421/1‑150 (MQ=60)
                                                                                                              TTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATC                                        >  SRR19973063.4733527/1‑150 (MQ=60)
                                                                                                                 GCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAAT                                     <  SRR19973063.2703869/150‑1 (MQ=60)
                                                                                                                 GCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAAT                                     <  SRR19973063.4151169/150‑1 (MQ=60)
                                                                                                                  CCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATA                                    >  SRR19973063.1651490/1‑150 (MQ=60)
                                                                                                                   CCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAA                                   <  SRR19973063.455071/150‑1 (MQ=60)
                                                                                                                   CATTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAA                                   >  SRR19973063.473703/1‑150 (MQ=60)
                                                                                                                     TTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACA                                 >  SRR19973063.358724/1‑150 (MQ=60)
                                                                                                                       CACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATC                               >  SRR19973063.231742/1‑150 (MQ=60)
                                                                                                                       CACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATC                               >  SRR19973063.231769/1‑150 (MQ=60)
                                                                                                                       CACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATC                               >  SRR19973063.4204895/1‑150 (MQ=60)
                                                                                                                        ACCACATCACTTTTTTTAGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACTTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCT                              <  SRR19973063.946741/150‑1 (MQ=60)
                                                                                                                          CACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGT                            >  SRR19973063.3273980/1‑150 (MQ=60)
                                                                                                                           ACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTA                           >  SRR19973063.505602/1‑150 (MQ=60)
                                                                                                                            CATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTAC                          <  SRR19973063.1802396/150‑1 (MQ=60)
                                                                                                                            CATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTAC                          <  SRR19973063.351550/150‑1 (MQ=60)
                                                                                                                                   TTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAA                   >  SRR19973063.4697874/1‑150 (MQ=60)
                                                                                                                                   TTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAA                   >  SRR19973063.1031031/1‑150 (MQ=60)
                                                                                                                                    TTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAAC                  <  SRR19973063.1448059/150‑1 (MQ=60)
                                                                                                                                     TTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACG                 <  SRR19973063.2478148/150‑1 (MQ=60)
                                                                                                                                     TTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACG                 <  SRR19973063.2842474/150‑1 (MQ=60)
                                                                                                                                      TGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGC                >  SRR19973063.1168121/1‑150 (MQ=60)
                                                                                                                                       GTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCA               <  SRR19973063.66034/150‑1 (MQ=60)
                                                                                                                                       GTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCA               >  SRR19973063.616826/1‑150 (MQ=60)
                                                                                                                                        TCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAT              >  SRR19973063.1780134/1‑150 (MQ=60)
                                                                                                                                        TCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAT              >  SRR19973063.1780132/1‑150 (MQ=60)
                                                                                                                                          GCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATAC            <  SRR19973063.379831/150‑1 (MQ=60)
                                                                                                                                           CTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACG           >  SRR19973063.1734119/1‑150 (MQ=60)
                                                                                                                                            TGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGA          <  SRR19973063.357008/150‑1 (MQ=60)
                                                                                                                                            TGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGA          >  SRR19973063.2757524/1‑150 (MQ=60)
                                                                                                                                                 GGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTC     >  SRR19973063.3634360/1‑150 (MQ=60)
                                                                                                                                                 GGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTC     >  SRR19973063.1045485/1‑150 (MQ=60)
                                                                                                                                                    TAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGT  <  SRR19973063.5019104/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACTCTGT  >  CP009273/581485‑581782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑