Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,730,042	T→G	79.8%	S180A (TCC→GCC)	bamD →	BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,730,042	0	T	G	79.8%	689.5 / 122.4	271	S180A (TCC→GCC)	bamD	BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand): ref base T (23/32); new base G (99/117); total (122/149)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 273 total aligned reads displayed.

GATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  CP009273/2729895‑2730191

 aTCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCg                                                                                                                                                    <  2:3670301/150‑1 (MQ=255)

 aTCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTACAAACTTGTGCGCGGCTATCCGAACAGTCAGTACACCACAGATGCCAACAAACGTATGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCg                                                                                                                                                    <  1:3533868/150‑1 (MQ=255)

    gTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTgg                                                                                                                                                 >  2:4961402/1‑150 (MQ=255)

    gTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTgg                                                                                                                                                 <  1:1555607/150‑1 (MQ=255)

     tAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGc                                                                                                                                                >  1:2061849/1‑150 (MQ=255)

      aGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGcc                                                                                                                                               <  2:1257439/150‑1 (MQ=255)

      aGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGcc                                                                                                                                               >  1:2518764/1‑150 (MQ=255)

      aGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGcc                                                                                                                                               >  1:4586085/1‑150 (MQ=255)

      aGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGcc                                                                                                                                               <  1:4934347/150‑1 (MQ=255)

      aGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGcc                                                                                                                                               >  2:4465608/1‑150 (MQ=255)

         gatcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAg                                                                                                                                            >  1:2202801/1‑150 (MQ=255)

         gatcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAg                                                                                                                                            <  2:336510/150‑1 (MQ=255)

         gatcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAg                                                                                                                                            >  1:4209880/1‑150 (MQ=255)

         gatcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAg                                                                                                                                            <  1:2286865/150‑1 (MQ=255)

         gatcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAg                                                                                                                                            >  1:1049929/1‑150 (MQ=255)

             gcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACt                                                                                                                                        <  1:1637700/150‑1 (MQ=255)

              cgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACta                                                                                                                                       >  2:4581167/1‑150 (MQ=255)

               gatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtat                                                                                                                                      <  2:1144224/150‑1 (MQ=255)

               gatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtat                                                                                                                                      >  1:5075722/1‑150 (MQ=255)

                 tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATAc                                                                                                                                    >  2:2758656/1‑150 (MQ=255)

                  ccTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACa                                                                                                                                   >  1:713339/1‑150 (MQ=255)

                        cATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGt                                                                                                                             >  1:1894268/1‑150 (MQ=255)

                          tGCATGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGAAGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTgg                                                                                                                           >  1:4947925/1‑150 (MQ=255)

                          tGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTgg                                                                                                                           >  1:4795917/1‑150 (MQ=255)

                           gCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTgga                                                                                                                          <  2:2384591/150‑2 (MQ=255)

                             aCGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGgcgc                                                                                                                        <  2:3426301/150‑1 (MQ=255)

                              cGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCa                                                                                                                       >  1:2513719/1‑150 (MQ=255)

                               gAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGAGGCGCAt                                                                                                                      >  2:4326681/1‑150 (MQ=255)

                               gAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCAt                                                                                                                      >  1:1217221/1‑150 (MQ=255)

                                aGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATg                                                                                                                     <  1:796637/150‑1 (MQ=255)

                                 gCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATgg                                                                                                                    >  2:1627222/1‑150 (MQ=255)

                                  cTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATggg                                                                                                                   >  2:932942/1‑150 (MQ=255)

                                     cGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                                >  2:2446097/1‑150 (MQ=255)

                                       tttAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGcc                                                                                                              >  1:660145/1‑150 (MQ=255)

                                        ttAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcg                                                                                                             >  2:223232/1‑150 (MQ=255)

                                         tAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcgt                                                                                                            >  2:304045/1‑150 (MQ=255)

                                           gTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcgtcg                                                                                                          <  1:2140824/150‑1 (MQ=255)

                                           gTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcgtcg                                                                                                          <  2:4696719/150‑1 (MQ=255)

                                              aCTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTa                                                                                                       <  2:2972987/150‑1 (MQ=255)

                                                  ttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcg                                                                                                   >  1:529528/1‑150 (MQ=255)

                                                   tCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcgc                                                                                                  <  2:54820/150‑1 (MQ=255)

                                                   tCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcgc                                                                                                  >  2:2939278/1‑150 (MQ=255)

                                                               cgcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATg                                                                                      >  2:2846333/1‑150 (MQ=255)

                                                               cgcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATg                                                                                      >  1:3839855/1‑150 (MQ=255)

                                                               cgcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATg                                                                                      <  2:2651058/150‑1 (MQ=255)

                                                                 cgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGtt                                                                                    <  1:595772/150‑1 (MQ=255)

                                                                   gCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTgc                                                                                  <  2:354585/150‑1 (MQ=255)

                                                                   gCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTgc                                                                                  >  1:4152201/1‑150 (MQ=255)

                                                                    cTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTgcg                                                                                 >  2:594623/1‑150 (MQ=255)

                                                                      agccGAACAGTCAGTACACCACCGAGGCCACCAAACGTCTGGTAGTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGcgcg                                                                               <  2:2942345/148‑1 (MQ=255)

                                                                      aTCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGTCGAAATATGAATACTCCGTGGACGAGTTTTATACATATAGTTTCTCTTTTGTTGCCGTCGTTAACCGAGTAGATGGCTTGTTGcgcg                                                                               <  2:4060608/150‑1 (MQ=255)

                                                                        ccGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGAc                                                                             <  1:2162812/150‑1 (MQ=255)

                                                                         cGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACt                                                                            >  1:193738/1‑150 (MQ=255)

                                                                             cagtcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTAccc                                                                        <  2:2902662/150‑1 (MQ=255)

                                                                             cagtcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTAccc                                                                        <  1:3623328/150‑1 (MQ=255)

                                                                              agtcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCg                                                                       <  2:3079514/150‑1 (MQ=255)

                                                                               gtcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCgg                                                                      <  2:3281066/150‑1 (MQ=255)

                                                                                   gtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAc                                                                  <  2:3003551/150‑1 (MQ=255)

                                                                                     acacCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcnc                                                                <  1:2440478/150‑1 (MQ=255)

                                                                                      caccacCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCa                                                               <  2:5072838/150‑1 (MQ=255)

                                                                                      caccacCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCa                                                               <  1:3295739/150‑1 (MQ=255)

                                                                                       accaccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAg                                                              >  1:2959677/1‑150 (MQ=255)

                                                                                         caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGc                                                            <  2:4872131/150‑1 (MQ=255)

                                                                                            cGATGCCACCAAACGTCTGGTAGTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTAc                                                         <  2:716594/150‑1 (MQ=255)

                                                                                             gATGCCACCAAACGTCTGGTATTCCTGAAAGAACGTCTGGCGAAATATGAATACGCCCTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAACCAGGCTAcg                                                        >  1:1386733/1‑150 (MQ=255)

                                                                                                gCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTATAAGGCATGTTGAGCTACTACCCGGATACCCAGGCTACGCGt                                                     >  1:1012388/1‑150 (MQ=255)

                                                                                                 ccaccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTg                                                    <  1:929663/150‑1 (MQ=255)

                                                                                                  caccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGa                                                   <  1:1673518/150‑1 (MQ=255)

                                                                                                    ccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATg                                                 <  2:1322435/150‑1 (MQ=255)

                                                                                                    ccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATg                                                 <  2:3902927/150‑1 (MQ=255)

                                                                                                    ccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATg                                                 >  1:2207001/1‑150 (MQ=255)

                                                                                                         cGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTg                                            >  2:729630/1‑150 (MQ=255)

                                                                                                            cTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCg                                         <  2:1996105/150‑1 (MQ=255)

                                                                                                               gTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTg                                      <  2:2061676/150‑1 (MQ=255)

                                                                                                                tATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGa                                     <  1:3114479/150‑1 (MQ=255)

                                                                                                                  ttCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATg                                   <  1:3012870/150‑1 (MQ=255)

                                                                                                                   tCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATgg                                  <  2:2330895/150‑1 (MQ=255)

                                                                                                                   tCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATgg                                  <  2:2331098/150‑1 (MQ=255)

                                                                                                                    ccTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGa                                 >  2:253876/1‑150 (MQ=255)

                                                                                                                    ccTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGa                                 <  1:4301009/150‑1 (MQ=255)

                                                                                                                    ccTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGa                                 >  1:4959124/1‑150 (MQ=255)

                                                                                                                       gAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCTGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaaaa                              >  1:1324961/1‑150 (MQ=255)

                                                                                                                       gAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaaaa                              <  2:322364/150‑1 (MQ=255)

                                                                                                                        aaaGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAAt                             >  2:889012/1‑150 (MQ=255)

                                                                                                                         aaGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCACTGCCGCTGATGGACAATg                            >  1:4825187/1‑150 (MQ=255)

                                                                                                                              cGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAc                       <  1:1696802/150‑1 (MQ=255)

                                                                                                                               gTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAcc                      >  1:287048/1‑150 (MQ=255)

                                                                                                                               gTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAcc                      >  1:425093/1‑150 (MQ=255)

                                                                                                                                  tGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTc                   >  2:2145663/1‑150 (MQ=255)

                                                                                                                                   ggCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTca                  <  2:1482458/150‑1 (MQ=255)

                                                                                                                                   ggCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTca                  <  2:3950543/150‑1 (MQ=255)

                                                                                                                                      gAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagat               <  2:4177551/150‑1 (MQ=255)

                                                                                                                                      gAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagat               >  2:2964221/1‑150 (MQ=255)

                                                                                                                                          tatGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCTTGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAACATGCATACCGTcagatgcag           >  2:3749189/1‑150 (MQ=255)

                                                                                                                                          tatGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatgcaa           <  2:3774859/150‑2 (MQ=255)

                                                                                                                                             gAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatgcagatg        <  1:872912/150‑1 (MQ=255)

                                                                                                                                              aaTACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGa       >  1:3184174/1‑150 (MQ=255)

                                                                                                                                                tACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAAt     <  1:4441126/150‑1 (MQ=255)

                                                                                                                                                   tCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATgcg  >  1:3657283/1‑150 (MQ=255)

                                                                                                                                                   tCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATgcg  >  1:2801671/1‑150 (MQ=255)

GATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  CP009273/2729895‑2730191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  CP009273/2729893‑2730191

TCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACG                                                                                                                                                       <  SRR19973063.1723411/150‑1 (MQ=60)

 CGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGC                                                                                                                                                      >  SRR19973063.4060812/1‑150 (MQ=60)

  GATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCC                                                                                                                                                     <  SRR19973063.2826177/150‑1 (MQ=60)

   ATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCG                                                                                                                                                    >  SRR19973063.323302/1‑150 (MQ=60)

   ATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTACAAACTTGTGCGCGGCTATCCGAACAGTCAGTACACCACAGATGCCAACAAACGTATGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCG                                                                                                                                                    <  SRR19973063.3545192/150‑1 (MQ=60)

      GTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGG                                                                                                                                                 <  SRR19973063.1560372/150‑1 (MQ=60)

      GTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGG                                                                                                                                                 <  SRR19973063.3313942/150‑1 (MQ=60)

       TAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGC                                                                                                                                                >  SRR19973063.2068118/1‑150 (MQ=60)

       TAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGC                                                                                                                                                >  SRR19973063.2068291/1‑150 (MQ=60)

       TAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGC                                                                                                                                                <  SRR19973063.217691/150‑1 (MQ=60)

       TAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGC                                                                                                                                                >  SRR19973063.1037121/1‑150 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               >  SRR19973063.4600762/1‑150 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               <  SRR19973063.4908458/150‑1 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               <  SRR19973063.4950288/150‑1 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               >  SRR19973063.2526801/1‑150 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               >  SRR19973063.2526793/1‑150 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               <  SRR19973063.4950353/150‑1 (MQ=60)

        AGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCC                                                                                                                                               <  SRR19973063.4950490/150‑1 (MQ=60)

         GCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCG                                                                                                                                              >  SRR19973063.2159336/1‑150 (MQ=60)

         GCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCG                                                                                                                                              <  SRR19973063.2193883/150‑1 (MQ=60)

          CGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGA                                                                                                                                             <  SRR19973063.434368/150‑1 (MQ=60)

          CGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGA                                                                                                                                             <  SRR19973063.2984505/150‑1 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAG                                                                                                                                            >  SRR19973063.2209733/1‑150 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            >  SRR19973063.2378921/1‑150 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            <  SRR19973063.3179961/150‑1 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            <  SRR19973063.2294163/150‑1 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            >  SRR19973063.4223147/1‑150 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            >  SRR19973063.4223308/1‑150 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            <  SRR19973063.1322983/150‑1 (MQ=60)

           GATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAG                                                                                                                                            >  SRR19973063.1053104/1‑150 (MQ=60)

            ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCACACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGT                                                                                                                                           <  SRR19973063.3884644/150‑1 (MQ=60)

            ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGT                                                                                                                                           >  SRR19973063.3646581/1‑150 (MQ=60)

             TCGCGATCCTCAACATTCACGATCTGAGTTTAGTGACTTTTCCAAACTGGTGAGCGTCTATCAGAACAGTCAGTACACCACCTATGCCACCAAACGTCTGTTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTA                                                                                                                                          <  SRR19973063.663381/150‑1 (MQ=60)

               GCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACT                                                                                                                                        <  SRR19973063.1642709/150‑1 (MQ=60)

                 GATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTAT                                                                                                                                      >  SRR19973063.5092047/1‑150 (MQ=60)

                    CCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACA                                                                                                                                   >  SRR19973063.715434/1‑150 (MQ=60)

                    CCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACA                                                                                                                                   <  SRR19973063.2888374/150‑1 (MQ=60)

                    CCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACA                                                                                                                                   >  SRR19973063.586523/1‑150 (MQ=60)

                          CATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGT                                                                                                                             >  SRR19973063.1900193/1‑150 (MQ=60)

                            TGCATGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGAAGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGG                                                                                                                           >  SRR19973063.4963929/1‑150 (MQ=60)

                            TGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGG                                                                                                                           >  SRR19973063.4811242/1‑150 (MQ=60)

                                CGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCA                                                                                                                       >  SRR19973063.2521595/1‑150 (MQ=60)

                                CGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCA                                                                                                                       >  SRR19973063.2521732/1‑150 (MQ=60)

                                 GAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCAT                                                                                                                      >  SRR19973063.1220866/1‑150 (MQ=60)

                                  AGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATG                                                                                                                     >  SRR19973063.4186545/1‑150 (MQ=60)

                                  AGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATG                                                                                                                     <  SRR19973063.798935/150‑1 (MQ=60)

                                   GCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGG                                                                                                                    <  SRR19973063.934061/150‑1 (MQ=60)

                                     TGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGT                                                                                                                  <  SRR19973063.4458444/150‑1 (MQ=60)

                                      GCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTT                                                                                                                 <  SRR19973063.556506/150‑1 (MQ=60)

                                       CGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTG                                                                                                                <  SRR19973063.86473/150‑1 (MQ=60)

                                         TTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCC                                                                                                              >  SRR19973063.2061850/1‑150 (MQ=60)

                                         TTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCC                                                                                                              >  SRR19973063.662077/1‑150 (MQ=60)

                                         TTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCC                                                                                                              >  SRR19973063.662079/1‑150 (MQ=60)

                                          TTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCG                                                                                                             >  SRR19973063.274619/1‑150 (MQ=60)

                                            AGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTC                                                                                                           >  SRR19973063.4825635/1‑150 (MQ=60)

                                             GTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCG                                                                                                          <  SRR19973063.2147506/150‑1 (MQ=60)

                                                    TTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCG                                                                                                   >  SRR19973063.531083/1‑150 (MQ=60)

                                                       CAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGT                                                                                                <  SRR19973063.3329333/150‑1 (MQ=60)

                                                           CTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAA                                                                                            >  SRR19973063.2608181/1‑150 (MQ=60)

                                                            TGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAG                                                                                           >  SRR19973063.4223625/1‑150 (MQ=60)

                                                            TGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAG                                                                                           >  SRR19973063.1130017/1‑150 (MQ=60)

                                                             GGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGG                                                                                          <  SRR19973063.2862338/150‑1 (MQ=60)

                                                             GGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGG                                                                                          >  SRR19973063.5000131/1‑150 (MQ=60)

                                                                 CGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATG                                                                                      >  SRR19973063.3852066/1‑150 (MQ=60)

                                                                   CGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTT                                                                                    <  SRR19973063.597526/150‑1 (MQ=60)

                                                                     GCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGC                                                                                  >  SRR19973063.4165479/1‑150 (MQ=60)

                                                                        ATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCG                                                                               <  SRR19973063.1916814/150‑1 (MQ=60)

                                                                         TCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGA                                                                              <  SRR19973063.3064435/150‑1 (MQ=60)

                                                                          CCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGAC                                                                             <  SRR19973063.2169584/150‑1 (MQ=60)

                                                                           CGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACT                                                                            <  SRR19973063.5136097/150‑1 (MQ=60)

                                                                           CGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACT                                                                            <  SRR19973063.4553832/150‑1 (MQ=60)

                                                                           CGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACT                                                                            >  SRR19973063.194330/1‑150 (MQ=60)

                                                                            GAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTA                                                                           <  SRR19973063.4938021/150‑1 (MQ=60)

                                                                               CAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCC                                                                        <  SRR19973063.3634956/150‑1 (MQ=60)

                                                                               CAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCC                                                                        <  SRR19973063.3635049/150‑1 (MQ=60)

                                                                                 GTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGG                                                                      <  SRR19973063.889574/150‑1 (MQ=60)

                                                                                     GTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAC                                                                  <  SRR19973063.1652406/150‑1 (MQ=60)

                                                                                       ACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACNC                                                                <  SRR19973063.2448277/150‑1 (MQ=60)

                                                                                        CACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCA                                                               <  SRR19973063.3306293/150‑1 (MQ=60)

                                                                                         ACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAG                                                              >  SRR19973063.2969141/1‑150 (MQ=60)

                                                                                           CACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGC                                                            >  SRR19973063.2414502/1‑150 (MQ=60)

                                                                                               GATGCCACCAAACGTCTGGTATTCCTGAAAGAACGTCTGGCGAAATATGAATACGCCCTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAACCAGGCTACG                                                        >  SRR19973063.1390925/1‑150 (MQ=60)

                                                                                                 TGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCG                                                      >  SRR19973063.4773670/1‑150 (MQ=60)

                                                                                                  GCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTATAAGGCATGTTGAGCTACTACCCGGATACCCAGGCTACGCGT                                                     >  SRR19973063.1015440/1‑150 (MQ=60)

                                                                                                   CCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTG                                                    >  SRR19973063.2149181/1‑150 (MQ=60)

                                                                                                   CCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTG                                                    <  SRR19973063.5140610/150‑1 (MQ=60)

                                                                                                   CCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTG                                                    <  SRR19973063.1340210/150‑1 (MQ=60)

                                                                                                   CCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTG                                                    <  SRR19973063.932434/150‑1 (MQ=60)

                                                                                                    CACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGA                                                   <  SRR19973063.1678618/150‑1 (MQ=60)

                                                                                                     ACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGAT                                                  <  SRR19973063.1629293/150‑1 (MQ=60)

                                                                                                      CCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG                                                 <  SRR19973063.1910234/150‑1 (MQ=60)

                                                                                                      CCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG                                                 >  SRR19973063.2213952/1‑150 (MQ=60)

                                                                                                      CCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG                                                 >  SRR19973063.2213937/1‑150 (MQ=60)

                                                                                                       CAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGC                                                >  SRR19973063.3603708/1‑150 (MQ=60)

                                                                                                       CAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGC                                                >  SRR19973063.1679673/1‑150 (MQ=60)

                                                                                                        AAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCG                                               >  SRR19973063.1273559/1‑150 (MQ=60)

                                                                                                          ACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCT                                             <  SRR19973063.4630027/150‑1 (MQ=60)

                                                                                                                 GTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTG                                      >  SRR19973063.309631/1‑150 (MQ=60)

                                                                                                                  TATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGA                                     <  SRR19973063.3124505/150‑1 (MQ=60)

                                                                                                                    TTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATG                                   <  SRR19973063.3022480/150‑1 (MQ=60)

                                                                                                                      CCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGA                                 <  SRR19973063.4314676/150‑1 (MQ=60)

                                                                                                                      CCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGA                                 >  SRR19973063.4975157/1‑150 (MQ=60)

                                                                                                                        TGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAA                               <  SRR19973063.1887713/150‑1 (MQ=60)

                                                                                                                        TGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAA                               <  SRR19973063.1605559/150‑1 (MQ=60)

                                                                                                                         GAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCTGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAA                              >  SRR19973063.1328924/1‑150 (MQ=60)

                                                                                                                          AAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTAAACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGAGATGCGCTGCCGCTGAAGGAAAAT                             >  SRR19973063.816647/1‑150 (MQ=60)

                                                                                                                           AAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATG                            >  SRR19973063.1667926/1‑150 (MQ=60)

                                                                                                                           AAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCACTGCCGCTGATGGACAATG                            >  SRR19973063.4840644/1‑150 (MQ=60)

                                                                                                                            AGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGC                           <  SRR19973063.294474/150‑1 (MQ=60)

                                                                                                                             GATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCA                          <  SRR19973063.223897/150‑1 (MQ=60)

                                                                                                                             GATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCA                          <  SRR19973063.222991/150‑1 (MQ=60)

                                                                                                                               TCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATA                        <  SRR19973063.658778/150‑1 (MQ=60)

                                                                                                                                CGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAC                       <  SRR19973063.1701974/150‑1 (MQ=60)

                                                                                                                                 GTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACC                      >  SRR19973063.287897/1‑150 (MQ=60)

                                                                                                                                 GTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACC                      >  SRR19973063.3135218/1‑150 (MQ=60)

                                                                                                                                 GTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACC                      >  SRR19973063.426283/1‑150 (MQ=60)

                                                                                                                                    TGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTC                   >  SRR19973063.2103308/1‑150 (MQ=60)

                                                                                                                                    TGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTC                   <  SRR19973063.1501059/150‑1 (MQ=60)

                                                                                                                                     GGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCA                  >  SRR19973063.4043706/1‑150 (MQ=60)

                                                                                                                                     GGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCA                  >  SRR19973063.4971536/1‑150 (MQ=60)

                                                                                                                                     GGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCA                  <  SRR19973063.4224217/150‑1 (MQ=60)

                                                                                                                                       CGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGA                >  SRR19973063.1731669/1‑150 (MQ=60)

                                                                                                                                       CGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGA                <  SRR19973063.3619653/150‑1 (MQ=60)

                                                                                                                                        GAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGAT               >  SRR19973063.5019113/1‑150 (MQ=60)

                                                                                                                                        GAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGAT               >  SRR19973063.746405/1‑150 (MQ=60)

                                                                                                                                            TATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCTTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCTGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGTAAAATGCATACCGTCAGATGCAG           <  SRR19973063.2453910/150‑1 (MQ=60)

                                                                                                                                            TATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAG           <  SRR19973063.1177887/150‑1 (MQ=60)

                                                                                                                                              TGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGAT         <  SRR19973063.4290901/150‑1 (MQ=60)

                                                                                                                                              TGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGAT         <  SRR19973063.373359/150‑1 (MQ=60)

                                                                                                                                              TGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGAT         <  SRR19973063.1415845/150‑1 (MQ=60)

                                                                                                                                               GAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATG        >  SRR19973063.4381379/1‑150 (MQ=60)

                                                                                                                                               GAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATG        <  SRR19973063.875421/150‑1 (MQ=60)

                                                                                                                                                AATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGA       >  SRR19973063.3194410/1‑150 (MQ=60)

                                                                                                                                                  TACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAAT     <  SRR19973063.4455377/150‑1 (MQ=60)

                                                                                                                                                  TACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAAT     <  SRR19973063.737871/150‑1 (MQ=60)

                                                                                                                                                     TCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  SRR19973063.3669010/1‑150 (MQ=60)

                                                                                                                                                     TCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  SRR19973063.2810678/1‑150 (MQ=60)

TCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  CP009273/2729893‑2730191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`