Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,730,042 T→G 90.6% S180A (TCC→GCC)  bamD → BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,730,0420TG90.6% 600.4 / 25.4 170S180A (TCC→GCC) bamDBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (7/9);  new base G (81/73);  total (88/82)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

CGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGA  >  CP009273/2729898‑2730186
                                                                                                                                                |                                                                                                                                                
      gatcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCg                                                                                                                                         <  2:1355865/148‑1 (MQ=255)
       atcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGa                                                                                                                                        >  2:1821411/1‑148 (MQ=255)
        tcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAg                                                                                                                                       >  2:950909/1‑148 (MQ=255)
        tcgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAg                                                                                                                                       <  2:3478411/148‑1 (MQ=255)
         cgcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGt                                                                                                                                      >  2:1340079/1‑148 (MQ=255)
          gcgatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTa                                                                                                                                     <  1:2609349/148‑1 (MQ=255)
            gatcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACt                                                                                                                                   <  2:926120/148‑1 (MQ=255)
             atcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACta                                                                                                                                  <  1:1116507/148‑1 (MQ=255)
              tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtat                                                                                                                                 >  2:3161672/1‑148 (MQ=255)
              tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtat                                                                                                                                 >  1:2375011/1‑148 (MQ=255)
               ccTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtata                                                                                                                                >  2:1009787/1‑148 (MQ=255)
               ccTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtata                                                                                                                                <  2:789097/148‑1 (MQ=255)
               ccTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACtata                                                                                                                                <  1:643755/148‑1 (MQ=255)
                  cAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAg                                                                                                                             >  2:1118274/1‑148 (MQ=255)
                   aaCATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGa                                                                                                                            <  2:1241197/148‑1 (MQ=255)
                    aCATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGaa                                                                                                                           >  1:992095/1‑148 (MQ=255)
                     cATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAAc                                                                                                                          >  2:3406500/1‑148 (MQ=255)
                     cATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAAc                                                                                                                          >  2:3406747/1‑148 (MQ=255)
                      aTGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACg                                                                                                                         <  1:3243369/148‑1 (MQ=255)
                      aTGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACg                                                                                                                         <  1:3242373/148‑1 (MQ=255)
                        gCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTg                                                                                                                       >  1:3376821/1‑148 (MQ=255)
                        gCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTg                                                                                                                       >  1:1999295/1‑148 (MQ=255)
                        gCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTg                                                                                                                       >  1:1957644/1‑148 (MQ=255)
                           cGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATCTGAATACGCCGTGGCCGAGTACTATACAGAACGTGgcg                                                                                                                    >  1:132673/1‑148 (MQ=255)
                           cGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGgcg                                                                                                                    >  1:1237991/1‑148 (MQ=255)
                              gCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCAt                                                                                                                 <  2:2078224/148‑1 (MQ=255)
                                 gCGTTTAGTGACTTTTCCAAACTGGTGCGCGTCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATggg                                                                                                              <  1:2566691/148‑1 (MQ=255)
                                 gCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATggg                                                                                                              <  1:2567422/148‑1 (MQ=255)
                                     ttAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGc                                                                                                          <  2:1829413/148‑1 (MQ=255)
                                     ttAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGc                                                                                                          <  2:763466/148‑1 (MQ=255)
                                       aGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcg                                                                                                        >  2:2578254/1‑148 (MQ=255)
                                         tGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcgtc                                                                                                      >  2:3351970/1‑148 (MQ=255)
                                          gACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcgtcg                                                                                                     >  2:2406243/1‑148 (MQ=255)
                                           aCTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCcgtcgt                                                                                                    <  2:639850/148‑1 (MQ=255)
                                             ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTa                                                                                                  >  1:3362927/1‑148 (MQ=255)
                                             ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTa                                                                                                  <  1:1087482/148‑1 (MQ=255)
                                               ttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAAc                                                                                                <  2:155876/148‑1 (MQ=255)
                                                   aaaCTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcgcg                                                                                            >  2:3166812/1‑148 (MQ=255)
                                                   aaaCTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcgcg                                                                                            >  1:1588557/1‑148 (MQ=255)
                                                       tGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGa                                                                                        >  1:877254/1‑148 (MQ=255)
                                                       tGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGa                                                                                        >  1:877190/1‑148 (MQ=255)
                                                       tGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGa                                                                                        >  1:2654323/1‑148 (MQ=255)
                                                         gTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAACGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAg                                                                                      <  2:3508663/148‑1 (MQ=255)
                                                           gcgcGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGc                                                                                    <  2:974292/148‑1 (MQ=255)
                                                             gcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCAt                                                                                  <  2:473086/148‑1 (MQ=255)
                                                             gcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCAt                                                                                  <  1:1271633/148‑1 (MQ=255)
                                                                gCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGtt                                                                               >  1:1156110/1‑148 (MQ=255)
                                                                 cTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTg                                                                              >  2:1906654/1‑148 (MQ=255)
                                                                         aCAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTa                                                                      <  2:437428/148‑1 (MQ=255)
                                                                           agtcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTAcc                                                                    >  1:2499572/1‑148 (MQ=255)
                                                                            gtcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTAccc                                                                   <  2:2728425/148‑1 (MQ=255)
                                                                              cagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCgg                                                                 <  2:269052/148‑1 (MQ=255)
                                                                               agtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGa                                                                <  2:1373967/148‑1 (MQ=255)
                                                                               agtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGa                                                                <  1:1175225/148‑1 (MQ=255)
                                                                                   caccacCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcc                                                            <  2:1028462/148‑1 (MQ=255)
                                                                                   caccacCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTAGACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcc                                                            <  2:2991797/148‑1 (MQ=255)
                                                                                    accaccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAccc                                                           >  1:2052562/1‑148 (MQ=255)
                                                                                     ccaccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCa                                                          >  2:2513639/1‑148 (MQ=255)
                                                                                       accGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAgg                                                        >  1:285920/1‑148 (MQ=255)
                                                                                        ccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGc                                                       <  1:2249406/148‑1 (MQ=255)
                                                                                         cGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCt                                                      <  1:1469769/148‑1 (MQ=255)
                                                                                         cGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCt                                                      >  1:897596/1‑148 (MQ=255)
                                                                                         cGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCt                                                      >  2:1475735/1‑148 (MQ=255)
                                                                                          gATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTa                                                     <  2:3453630/148‑1 (MQ=255)
                                                                                               caccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGt                                                <  2:2891210/148‑1 (MQ=255)
                                                                                                 ccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGa                                              <  1:302863/148‑1 (MQ=255)
                                                                                                 ccaAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGa                                              <  1:1811436/148‑1 (MQ=255)
                                                                                                    aaCGTCTTGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTAAACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATgc                                           >  1:3465513/1‑148 (MQ=255)
                                                                                                     aCGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATgcg                                          >  2:388345/1‑148 (MQ=255)
                                                                                                     aCGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATgcg                                          <  2:902552/148‑1 (MQ=255)
                                                                                                        tCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTg                                       >  1:72080/1‑148 (MQ=255)
                                                                                                           ggTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCg                                    <  1:1572454/148‑1 (MQ=255)
                                                                                                           ggTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCg                                    >  1:3175035/1‑148 (MQ=255)
                                                                                                            gTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGc                                   >  2:343173/1‑148 (MQ=255)
                                                                                                             tATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCt                                  <  2:845610/148‑1 (MQ=255)
                                                                                                              aTTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTg                                 <  1:2287265/148‑1 (MQ=255)
                                                                                                               ttCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGa                                >  2:2346070/1‑148 (MQ=255)
                                                                                                                tCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGAt                               >  1:770072/1‑148 (MQ=255)
                                                                                                                   tGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGa                            <  1:1858129/148‑1 (MQ=255)
                                                                                                                   tGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGa                            <  1:2693685/148‑1 (MQ=255)
                                                                                                                   tGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGa                            >  2:3508079/1‑148 (MQ=255)
                                                                                                                    gAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaa                           >  2:3320762/1‑148 (MQ=255)
                                                                                                                    gAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaa                           >  2:2011159/1‑148 (MQ=255)
                                                                                                                    gAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaa                           >  2:2011101/1‑148 (MQ=255)
                                                                                                                    gAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaa                           >  2:2010854/1‑148 (MQ=255)
                                                                                                                      aaGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaaaa                         >  1:2381815/1‑148 (MQ=255)
                                                                                                                      aaGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGaaaa                         <  1:2422473/148‑1 (MQ=255)
                                                                                                                       aGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCCGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAAt                        >  2:2557054/1‑148 (MQ=255)
                                                                                                                        gATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATg                       >  2:420926/1‑148 (MQ=255)
                                                                                                                        gATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATg                       >  2:421927/1‑148 (MQ=255)
                                                                                                                           cGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCAt                    <  2:2907121/148‑1 (MQ=255)
                                                                                                                           cGTCTGGCGAAAGATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCAt                    <  1:2422094/148‑1 (MQ=255)
                                                                                                                             tCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAc                  >  1:3494766/1‑148 (MQ=255)
                                                                                                                             tCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAc                  >  2:2500870/1‑148 (MQ=255)
                                                                                                                                 gCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTc              <  2:1643374/148‑1 (MQ=255)
                                                                                                                                   gAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcag            <  1:31999/148‑1 (MQ=255)
                                                                                                                                      atatGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatg         <  2:2499572/148‑1 (MQ=255)
                                                                                                                                       tatGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatgc        >  1:3307669/1‑148 (MQ=255)
                                                                                                                                           aaTACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatgcagat    <  2:2439128/148‑1 (MQ=255)
                                                                                                                                             tACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGa  <  1:1295926/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGA  >  CP009273/2729898‑2730186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  CP009273/2729902‑2730191
                                                                                                                                            |                                                                                                                                                     
GCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCG                                                                                                                                              <  SRR19973065.1000385/150‑1 (MQ=60)
      GCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACT                                                                                                                                        <  SRR19973065.2625531/150‑1 (MQ=60)
      GCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACT                                                                                                                                        <  SRR19973065.2625559/150‑1 (MQ=60)
        GATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTAT                                                                                                                                      >  SRR19973065.2389837/1‑150 (MQ=60)
         ATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATA                                                                                                                                     <  SRR19973065.1123797/150‑1 (MQ=60)
           CCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACA                                                                                                                                   <  SRR19973065.647979/150‑1 (MQ=60)
           CCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACA                                                                                                                                   >  SRR19973065.2060945/1‑150 (MQ=60)
           CCTCAACACCCACGAGCTGCGTTTAGTGACCTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACA                                                                                                                                   <  SRR19973065.3069967/150‑1 (MQ=60)
              CAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAA                                                                                                                                >  SRR19973065.998685/1‑150 (MQ=60)
                  ATGCACGAGCTGCTTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGAGGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTG                                                                                                                            <  SRR19973065.3261948/150‑1 (MQ=60)
                  ATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTG                                                                                                                            <  SRR19973065.3262928/150‑1 (MQ=60)
                  ATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTG                                                                                                                            <  SRR19973065.3261918/150‑1 (MQ=60)
                  ATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTG                                                                                                                            >  SRR19973065.3397469/1‑150 (MQ=60)
                  ATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTG                                                                                                                            >  SRR19973065.2012239/1‑150 (MQ=60)
                  ATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTG                                                                                                                            >  SRR19973065.1970434/1‑150 (MQ=60)
                     CACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATCTGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCG                                                                                                                         >  SRR19973065.133814/1‑150 (MQ=60)
                     CACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCG                                                                                                                         >  SRR19973065.1246116/1‑150 (MQ=60)
                     CACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCG                                                                                                                         >  SRR19973065.1919060/1‑150 (MQ=60)
                         AGCTGCGTGTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATG                                                                                                                     >  SRR19973065.47432/1‑150 (MQ=60)
                             GCGTTTAGTGACTTTTCCAAACTGGTGCGCGTCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTT                                                                                                                 <  SRR19973065.2582721/150‑1 (MQ=60)
                             GCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTT                                                                                                                 <  SRR19973065.2583454/150‑1 (MQ=60)
                                       ACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTA                                                                                                       >  SRR19973065.1289381/1‑150 (MQ=60)
                                       ACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTA                                                                                                       >  SRR19973065.3383487/1‑150 (MQ=60)
                                         TTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAAC                                                                                                     <  SRR19973065.1094649/150‑1 (MQ=60)
                                         TTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAAC                                                                                                     >  SRR19973065.3499889/1‑150 (MQ=60)
                                             CCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCG                                                                                                 >  SRR19973065.1598754/1‑150 (MQ=60)
                                             CCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCG                                                                                                 >  SRR19973065.1599161/1‑150 (MQ=60)
                                                 ACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGA                                                                                             >  SRR19973065.2670720/1‑150 (MQ=60)
                                                 ACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGA                                                                                             >  SRR19973065.882852/1‑150 (MQ=60)
                                                 ACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGA                                                                                             >  SRR19973065.882834/1‑150 (MQ=60)
                                                 ACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGA                                                                                             >  SRR19973065.882788/1‑150 (MQ=60)
                                                     GTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGC                                                                                         <  SRR19973065.1251679/150‑1 (MQ=60)
                                                       GCGGGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGTAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCAT                                                                                       <  SRR19973065.3384305/150‑1 (MQ=60)
                                                         GCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGT                                                                                     <  SRR19973065.1280023/150‑1 (MQ=60)
                                                          CGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTT                                                                                    >  SRR19973065.1163533/1‑150 (MQ=60)
                                                          CGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTT                                                                                    >  SRR19973065.1163546/1‑150 (MQ=60)
                                                           GGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTG                                                                                   <  SRR19973065.2311991/150‑1 (MQ=60)
                                                           GGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTG                                                                                   <  SRR19973065.2602160/150‑1 (MQ=60)
                                                                     ACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACC                                                                         >  SRR19973065.2515292/1‑150 (MQ=60)
                                                                           AGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATA                                                                   <  SRR19973065.1182700/150‑1 (MQ=60)
                                                                              ACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCC                                                                >  SRR19973065.2065667/1‑150 (MQ=60)
                                                                                 CCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGG                                                             >  SRR19973065.287801/1‑150 (MQ=60)
                                                                                 CCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGG                                                             >  SRR19973065.287126/1‑150 (MQ=60)
                                                                                   ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCT                                                           >  SRR19973065.903337/1‑150 (MQ=60)
                                                                                    CCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTA                                                          <  SRR19973065.2263668/150‑1 (MQ=60)
                                                                                     CGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTAC                                                         <  SRR19973065.1479191/150‑1 (MQ=60)
                                                                                            ACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGAT                                                  <  SRR19973065.1125572/150‑1 (MQ=60)
                                                                                             CCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG                                                 <  SRR19973065.304849/150‑1 (MQ=60)
                                                                                             CCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG                                                 <  SRR19973065.1823472/150‑1 (MQ=60)
                                                                                             CCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG                                                 <  SRR19973065.1823355/150‑1 (MQ=60)
                                                                                              CAAACGTCTTGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTAAACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACAACCCGCATACCCAGGCTACGCGTGATGC                                                >  SRR19973065.3486618/1‑150 (MQ=60)
                                                                                                  CGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTG                                            >  SRR19973065.72831/1‑150 (MQ=60)
                                                                                                   GTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGC                                           >  SRR19973065.2440805/1‑150 (MQ=60)
                                                                                                    TCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCC                                          <  SRR19973065.325766/150‑1 (MQ=60)
                                                                                                     CTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCG                                         >  SRR19973065.3194233/1‑150 (MQ=60)
                                                                                                     CTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGAGACCCAGGCTACGCGTGATGCGCTGCCG                                         >  SRR19973065.2120681/1‑150 (MQ=60)
                                                                                                       GGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCT                                       <  SRR19973065.1582920/150‑1 (MQ=60)
                                                                                                          ATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGAT                                    <  SRR19973065.2301679/150‑1 (MQ=60)
                                                                                                          ATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGAT                                    >  SRR19973065.775192/1‑150 (MQ=60)
                                                                                                          ATTCCTGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGAT                                    >  SRR19973065.3357031/1‑150 (MQ=60)
                                                                                                               TGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAA                               <  SRR19973065.1870352/150‑1 (MQ=60)
                                                                                                               TGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAA                               <  SRR19973065.945156/150‑1 (MQ=60)
                                                                                                               TGAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAA                               <  SRR19973065.2710410/150‑1 (MQ=60)
                                                                                                                GAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAA                              >  SRR19973065.2395518/1‑150 (MQ=60)
                                                                                                                GAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAA                              >  SRR19973065.2396727/1‑150 (MQ=60)
                                                                                                                GAAAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAA                              >  SRR19973065.2396779/1‑150 (MQ=60)
                                                                                                                  AAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATG                            <  SRR19973065.804456/150‑1 (MQ=60)
                                                                                                                  AAGATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATG                            <  SRR19973065.2437752/150‑1 (MQ=60)
                                                                                                                     ATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCAT                         <  SRR19973065.1576503/150‑1 (MQ=60)
                                                                                                                     ATCGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCAT                         >  SRR19973065.2956710/1‑150 (MQ=60)
                                                                                                                       CGTCTGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAC                       >  SRR19973065.3516009/1‑150 (MQ=60)
                                                                                                                       CGTCTGGCGAAAGATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATAC                       <  SRR19973065.2437371/150‑1 (MQ=60)
                                                                                                                           TGGCGAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTC                   >  SRR19973065.133935/1‑150 (MQ=60)
                                                                                                                               GAAATATGAATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGAT               <  SRR19973065.32398/150‑1 (MQ=60)
                                                                                                                                 AATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGC             >  SRR19973065.3327828/1‑150 (MQ=60)
                                                                                                                                       AATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGA       >  SRR19973065.659539/1‑150 (MQ=60)
                                                                                                                                        ATACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAA      >  SRR19973065.1135272/1‑150 (MQ=60)
                                                                                                                                         TACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAAT     <  SRR19973065.2529757/150‑1 (MQ=60)
                                                                                                                                         TACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAAT     <  SRR19973065.1304464/150‑1 (MQ=60)
                                                                                                                                          ACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATG    <  SRR19973065.562814/150‑1 (MQ=60)
                                                                                                                                          ACGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATG    <  SRR19973065.562811/150‑1 (MQ=60)
                                                                                                                                           CGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGC   >  SRR19973065.636521/1‑150 (MQ=60)
                                                                                                                                           CGCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGC   >  SRR19973065.2409615/1‑150 (MQ=60)
                                                                                                                                            GCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  SRR19973065.1440720/1‑150 (MQ=60)
                                                                                                                                            GCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  SRR19973065.2524262/1‑150 (MQ=60)
                                                                                                                                            GCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  SRR19973065.426687/1‑150 (MQ=60)
                                                                                                                                            |                                                                                                                                                     
GCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCG  >  CP009273/2729902‑2730191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑