Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,743,438 C→A 100% intergenic (+19/+36) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,743,4380CA99.3% 904.5 / ‑2.5 272intergenic (+19/+36)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (141/129);  total (143/129)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

TCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGA  >  CP009273/2743292‑2743584
                                                                                                                                                  |                                                                                                                                                  
 cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCAt                                                                                                                                                <  2:2658810/150‑1 (MQ=255)
 cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCAt                                                                                                                                                <  2:3725866/150‑1 (MQ=255)
  ccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                               >  1:4705301/1‑150 (MQ=255)
    tGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCa                                                                                                                                             <  2:2489587/150‑1 (MQ=255)
      tCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAgg                                                                                                                                           >  2:3824231/1‑150 (MQ=255)
       cGCAGGCACCCGCGTGCGTATTGGCGAGTCCGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAggg                                                                                                                                          >  2:1190526/1‑150 (MQ=255)
       cGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAggg                                                                                                                                          >  2:1064713/1‑150 (MQ=255)
          aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                       <  2:1822788/150‑1 (MQ=255)
          aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                       >  1:4208533/1‑150 (MQ=255)
           ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGt                                                                                                                                      >  1:2164228/1‑150 (MQ=255)
            gCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTg                                                                                                                                     <  1:4191773/150‑1 (MQ=255)
             cACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGCCGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                    <  2:2565027/150‑1 (MQ=255)
             cACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                    >  2:4269819/1‑150 (MQ=255)
              aCCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGaa                                                                                                                                   <  2:2849613/150‑1 (MQ=255)
               cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAat                                                                                                                                  <  2:1649926/150‑1 (MQ=255)
                 cgcgTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAatta                                                                                                                                <  1:1306222/150‑1 (MQ=255)
                  gcgtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattat                                                                                                                               >  1:3562279/1‑150 (MQ=255)
                   cgtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattatt                                                                                                                              <  1:1461629/150‑1 (MQ=255)
                       cgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGa                                                                                                                          >  2:2411351/1‑150 (MQ=255)
                        gtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGaa                                                                                                                         >  1:5113855/1‑150 (MQ=255)
                           ttGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGc                                                                                                                      <  1:4462498/150‑1 (MQ=255)
                           gtGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATGAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGc                                                                                                                      <  2:4364138/149‑1 (MQ=255)
                              gctaGTACGATATCGATATTCTCGACTTACTGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGa                                                                                                                   <  1:439047/147‑1 (MQ=255)
                              gCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGa                                                                                                                   <  2:2154675/150‑1 (MQ=255)
                              gCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGa                                                                                                                   <  1:3028037/150‑1 (MQ=255)
                               cGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGaa                                                                                                                  >  2:2784091/1‑150 (MQ=255)
                                 aGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAt                                                                                                                >  2:493484/1‑150 (MQ=255)
                                      gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGc                                                                                                           >  1:2013124/1‑150 (MQ=255)
                                      gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGc                                                                                                           >  2:4649501/1‑150 (MQ=255)
                                        tatcgatatTCTCGACGTACAGGACAATATGATTAAGAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtt                                                                                                         >  2:2511472/1‑150 (MQ=255)
                                        tatcgatagTCTCGACGTACAGGACAAGATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtt                                                                                                         <  2:3160388/150‑1 (MQ=255)
                                          tcgatatTCTCGACGTACCGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtttt                                                                                                       <  2:197306/150‑1 (MQ=255)
                                           cgatatTCTCGACGTACAGGACACGATGATTAAGCAGGTAAAAGTTTTTCCTGTGACACCGCTGCGCGAGCGTGTGGCGGAGTACCGACAACGGCCCGGCCTTAGCATGCAGCGCCGTGAATTATTCCGAAAGCAGACATTCAGCgttgg                                                                                                      >  2:4392135/1‑148 (MQ=255)
                                              tatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCtt                                                                                                   <  2:317764/150‑1 (MQ=255)
                                               atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                  <  2:2611813/150‑1 (MQ=255)
                                                ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTc                                                                                                 <  2:633929/150‑1 (MQ=255)
                                                ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTc                                                                                                 >  1:3482588/1‑150 (MQ=255)
                                                ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTc                                                                                                 <  1:3150762/150‑1 (MQ=255)
                                                ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTc                                                                                                 <  2:5015309/150‑1 (MQ=255)
                                                    cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTTCTTTCGCTa                                                                                             >  1:913602/1‑150 (MQ=255)
                                                    cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                                                                                             <  2:3109523/150‑1 (MQ=255)
                                                       cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                          <  2:2252181/150‑1 (MQ=255)
                                                       cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                          <  1:752980/150‑1 (MQ=255)
                                                              gACAATATTATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                                   <  1:3685083/150‑1 (MQ=255)
                                                              gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                                   <  1:1414726/150‑1 (MQ=255)
                                                              gACAATATGATTAAGAAGGTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                                   <  1:3968990/150‑1 (MQ=255)
                                                                cAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg                                                                                 <  2:5141723/150‑1 (MQ=255)
                                                                 aaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                                >  1:404500/1‑150 (MQ=255)
                                                                       aTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                          >  1:380950/1‑150 (MQ=255)
                                                                        ttAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                         <  2:3364102/150‑1 (MQ=255)
                                                                           aGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                      <  1:688231/150‑1 (MQ=255)
                                                                           aGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                      >  2:2929049/1‑150 (MQ=255)
                                                                           aGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                      <  1:4247462/150‑1 (MQ=255)
                                                                           aGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                      >  1:1913713/1‑150 (MQ=255)
                                                                           aGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                      <  1:1534946/150‑1 (MQ=255)
                                                                             cAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                                    >  1:2428696/1‑150 (MQ=255)
                                                                               ggTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTAc                                                                  >  2:3551149/1‑150 (MQ=255)
                                                                                gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTaag                                                                 <  2:4545348/150‑3 (MQ=255)
                                                                                gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACg                                                                 <  1:4525991/150‑1 (MQ=255)
                                                                                 tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                >  2:4032133/1‑150 (MQ=255)
                                                                                 tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                >  2:4524315/1‑150 (MQ=255)
                                                                                  aaaaGTATTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                                               <  2:1280860/150‑1 (MQ=255)
                                                                                        ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCa                                                         >  1:1229536/1‑150 (MQ=255)
                                                                                        ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCa                                                         <  1:493484/150‑1 (MQ=255)
                                                                                        ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCa                                                         >  1:429854/1‑150 (MQ=255)
                                                                                         tttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAg                                                        >  1:2251285/1‑150 (MQ=255)
                                                                                         tttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAg                                                        >  1:2751745/1‑150 (MQ=255)
                                                                                            ccTGTGAAACCGCTGCGCGATAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGTCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                     <  2:4713991/150‑1 (MQ=255)
                                                                                             cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTTAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                    <  2:1400447/150‑1 (MQ=255)
                                                                                             cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                    <  2:550630/150‑1 (MQ=255)
                                                                                                tgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                 >  1:4258272/1‑150 (MQ=255)
                                                                                                 gAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAc                                                <  1:3255761/150‑1 (MQ=255)
                                                                                                  aaaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAcc                                               <  2:1935347/150‑1 (MQ=255)
                                                                                                  aaaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAcc                                               >  1:4660481/1‑150 (MQ=255)
                                                                                                    aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                             <  1:5039489/150‑1 (MQ=255)
                                                                                                    aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                             <  1:2650868/150‑1 (MQ=255)
                                                                                                    aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                             <  1:4792664/150‑1 (MQ=255)
                                                                                                     ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCtt                                            >  2:3403108/1‑150 (MQ=255)
                                                                                                     ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCtt                                            <  2:4974136/150‑1 (MQ=255)
                                                                                                       gCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                          >  2:5123825/1‑150 (MQ=255)
                                                                                                           cgcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                      <  2:1858849/150‑1 (MQ=255)
                                                                                                               agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                  >  1:2103670/1‑150 (MQ=255)
                                                                                                               agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                  >  1:1006173/1‑150 (MQ=255)
                                                                                                                 agTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGcc                                >  1:3365798/1‑150 (MQ=255)
                                                                                                                    gtgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAg                             >  1:3842604/1‑150 (MQ=255)
                                                                                                                         ggAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                        >  2:4144570/1‑150 (MQ=255)
                                                                                                                         ggAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                        >  1:3140873/1‑150 (MQ=255)
                                                                                                                           aGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTCCGTTa                      >  2:4541824/1‑150 (MQ=255)
                                                                                                                           aGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTa                      <  2:248333/150‑1 (MQ=255)
                                                                                                                             tAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAcc                    >  1:1872327/1‑150 (MQ=255)
                                                                                                                              aaCGAAAACGGCCCGGCATGAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGGACGTTACCt                   <  2:883052/150‑1 (MQ=255)
                                                                                                                               aCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTg                  >  2:731389/1‑150 (MQ=255)
                                                                                                                                cGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTgg                 >  1:3659518/1‑150 (MQ=255)
                                                                                                                                   aaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgcgc              >  1:3519429/1‑150 (MQ=255)
                                                                                                                                   aaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgcgc              >  1:2798100/1‑150 (MQ=255)
                                                                                                                                         cccGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc        <  2:4597425/150‑1 (MQ=255)
                                                                                                                                          ccGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca       >  2:27402/1‑150 (MQ=255)
                                                                                                                                          ccGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca       >  2:1383948/1‑150 (MQ=255)
                                                                                                                                           cGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcat      >  2:3964904/1‑150 (MQ=255)
                                                                                                                                             gCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatca    <  2:4777431/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGA  >  CP009273/2743292‑2743584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  CP009273/2743290‑2743587
                                                                                                                                                    |                                                                                                                                                     
GATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAG                                                                                                                                                      <  SRR19973063.4227062/150‑1 (MQ=60)
  TCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCA                                                                                                                                                    >  SRR19973063.1864656/1‑150 (MQ=60)
    CCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATG                                                                                                                                                  >  SRR19973063.4720294/1‑150 (MQ=60)
    CCTGGCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATG                                                                                                                                                  <  SRR19973063.150299/150‑1 (MQ=60)
            AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCG                                                                                                                                          >  SRR19973063.4097341/1‑150 (MQ=60)
            AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCG                                                                                                                                          >  SRR19973063.4221957/1‑150 (MQ=60)
             GGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGT                                                                                                                                         >  SRR19973063.2171007/1‑150 (MQ=60)
             GGCACCCGCGTGCGTATGGGCGAGTACGATATCGATATTCTCGACTTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGT                                                                                                                                         <  SRR19973063.1456682/150‑1 (MQ=60)
              GCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTG                                                                                                                                        <  SRR19973063.4229335/150‑1 (MQ=60)
              GCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTG                                                                                                                                        <  SRR19973063.4205224/150‑1 (MQ=60)
              GCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTG                                                                                                                                        <  SRR19973063.4205208/150‑1 (MQ=60)
              GCACCCGCGTGCGTATTGGCGAGTACGATATCGATAGTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTG                                                                                                                                        <  SRR19973063.4205158/150‑1 (MQ=60)
               CACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGA                                                                                                                                       >  SRR19973063.2141688/1‑150 (MQ=60)
                 CCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAAT                                                                                                                                     >  SRR19973063.4201230/1‑150 (MQ=60)
                   CGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTA                                                                                                                                   <  SRR19973063.1310111/150‑1 (MQ=60)
                    GCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTAT                                                                                                                                  >  SRR19973063.3573700/1‑150 (MQ=60)
                    GCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTAT                                                                                                                                  >  SRR19973063.868534/1‑150 (MQ=60)
                    GCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTAT                                                                                                                                  >  SRR19973063.3464781/1‑150 (MQ=60)
                     CGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATT                                                                                                                                 <  SRR19973063.1466089/150‑1 (MQ=60)
                      GTGCGTATTGGCGAGTACGATATCGATAGTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTA                                                                                                                                <  SRR19973063.647126/150‑1 (MQ=60)
                          GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGTCCGTGAATTATTACGAA                                                                                                                            >  SRR19973063.1252128/1‑150 (MQ=60)
                          GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973063.3536459/1‑150 (MQ=60)
                          GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973063.3406373/1‑150 (MQ=60)
                          GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973063.5130307/1‑150 (MQ=60)
                          GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973063.3536421/1‑150 (MQ=60)
                          GTATAGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973063.5130261/1‑150 (MQ=60)
                             TTGGCGAGTACTATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGC                                                                                                                         <  SRR19973063.4350770/150‑1 (MQ=60)
                             TTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGC                                                                                                                         <  SRR19973063.4476816/150‑1 (MQ=60)
                              TGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCA                                                                                                                        <  SRR19973063.3684178/150‑1 (MQ=60)
                               GGCGAGTACGATATCGTTATTCTCGACGTACTGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTTTGAAACCGCTGCGGGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAG                                                                                                                       <  SRR19973063.3955428/150‑1 (MQ=60)
                               GGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAG                                                                                                                       <  SRR19973063.693281/150‑1 (MQ=60)
                                GCTAGTACGATATCGATATTCTCGACTTACTGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGA                                                                                                                      <  SRR19973063.440273/150‑1 (MQ=60)
                                GCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGA                                                                                                                      <  SRR19973063.3037693/150‑1 (MQ=60)
                                  GAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCACTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAA                                                                                                                    >  SRR19973063.2871510/1‑150 (MQ=60)
                                  GAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCACTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAA                                                                                                                    >  SRR19973063.268961/1‑150 (MQ=60)
                                   AGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAT                                                                                                                   >  SRR19973063.1981543/1‑150 (MQ=60)
                                        GATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGC                                                                                                              >  SRR19973063.2019408/1‑150 (MQ=60)
                                         ATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCT                                                                                                             >  SRR19973063.2610405/1‑150 (MQ=60)
                                           ATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTT                                                                                                           <  SRR19973063.1483373/150‑1 (MQ=60)
                                           ATCGATATGCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTGTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTT                                                                                                           <  SRR19973063.4913316/150‑1 (MQ=60)
                                             CGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                         >  SRR19973063.3196735/1‑150 (MQ=60)
                                             CGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                         >  SRR19973063.3972635/1‑150 (MQ=60)
                                              GATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGC                                                                                                        >  SRR19973063.3076671/1‑150 (MQ=60)
                                                  TTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTC                                                                                                    <  SRR19973063.4776410/150‑1 (MQ=60)
                                                  TTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTC                                                                                                    >  SRR19973063.3493703/1‑150 (MQ=60)
                                                  TTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTC                                                                                                    <  SRR19973063.3160889/150‑1 (MQ=60)
                                                      TGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTA                                                                                                >  SRR19973063.2679628/1‑150 (MQ=60)
                                                      CGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTTCTTTCGCTA                                                                                                >  SRR19973063.916287/1‑150 (MQ=60)
                                                      CGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTA                                                                                                >  SRR19973063.2773559/1‑150 (MQ=60)
                                                      CGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTA                                                                                                <  SRR19973063.2032903/150‑1 (MQ=60)
                                                       GACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAC                                                                                               >  SRR19973063.3158237/1‑150 (MQ=60)
                                                        ACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACA                                                                                              >  SRR19973063.3282381/1‑150 (MQ=60)
                                                         CGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAG                                                                                             <  SRR19973063.2103603/150‑1 (MQ=60)
                                                         CGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAG                                                                                             <  SRR19973063.755176/150‑1 (MQ=60)
                                                          GTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGT                                                                                            <  SRR19973063.3849288/150‑1 (MQ=60)
                                                            ACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAA                                                                                          <  SRR19973063.734524/150‑1 (MQ=60)
                                                             CAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAC                                                                                         <  SRR19973063.902205/150‑1 (MQ=60)
                                                             CAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGTCGGAGTAACGAAAACGGACCGGCATTAGCATGCAGGTCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGATTTCTCTACACTAAC                                                                                         >  SRR19973063.2457594/1‑150 (MQ=60)
                                                                GACAATATTATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAT                                                                                      <  SRR19973063.3696881/150‑1 (MQ=60)
                                                                GACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAT                                                                                      >  SRR19973063.2153881/1‑150 (MQ=60)
                                                                GACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAT                                                                                      <  SRR19973063.3696811/150‑1 (MQ=60)
                                                                GACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAT                                                                                      <  SRR19973063.1419022/150‑1 (MQ=60)
                                                                GACAATATGATTAAGAAGGTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAT                                                                                      <  SRR19973063.3981691/150‑1 (MQ=60)
                                                                   AATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGC                                                                                   >  SRR19973063.405638/1‑150 (MQ=60)
                                                                    ATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCC                                                                                  <  SRR19973063.1404117/150‑1 (MQ=60)
                                                                     TATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCG                                                                                 <  SRR19973063.2206978/150‑1 (MQ=60)
                                                                       TGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAAAGGCCCGGCATTAGCATGCAGGGCCGTGACTTATTACTAAATCAGAAATTAAGCTGTTGCTTTCGCTACAGAAACCATCGCCGCA                                                                               >  SRR19973063.1818756/1‑150 (MQ=60)
                                                                         ATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACG                                                                             >  SRR19973063.382039/1‑150 (MQ=60)
                                                                          TTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGAGCGAGAGTGTGGCGGAGTAACGAAAACGGCCAGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGATTTTGCTTTCGCTACAGTAAACATCGCCGCACGA                                                                            >  SRR19973063.5025856/1‑150 (MQ=60)
                                                                           TAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAAAGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAATCTTTTGCTTTAGCTACAGTAACCATCGCCGCACGAC                                                                           >  SRR19973063.2895913/1‑150 (MQ=60)
                                                                             AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                         <  SRR19973063.4260996/150‑1 (MQ=60)
                                                                             AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                         <  SRR19973063.1539646/150‑1 (MQ=60)
                                                                             AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                         <  SRR19973063.4261006/150‑1 (MQ=60)
                                                                             AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                         >  SRR19973063.1919700/1‑150 (MQ=60)
                                                                             AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                         <  SRR19973063.690248/150‑1 (MQ=60)
                                                                               CAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGT                                                                       >  SRR19973063.2436458/1‑150 (MQ=60)
                                                                                AGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTA                                                                      <  SRR19973063.4335226/150‑1 (MQ=60)
                                                                                  GTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACG                                                                    <  SRR19973063.4540506/150‑1 (MQ=60)
                                                                                  GTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACG                                                                    >  SRR19973063.1068106/1‑150 (MQ=60)
                                                                                    AAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAC                                                                  <  SRR19973063.1892230/150‑1 (MQ=60)
                                                                                    AAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAC                                                                  <  SRR19973063.1892221/150‑1 (MQ=60)
                                                                                          TTTTCCTGTGAAACCGGTGCGCGAGAGTGTGGCGGTGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCA                                                            <  SRR19973063.494933/150‑1 (MQ=60)
                                                                                          TTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCA                                                            >  SRR19973063.431051/1‑150 (MQ=60)
                                                                                          TTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCA                                                            >  SRR19973063.1233210/1‑150 (MQ=60)
                                                                                          TTTGCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCA                                                            <  SRR19973063.494901/150‑1 (MQ=60)
                                                                                           TTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAG                                                           >  SRR19973063.2760620/1‑150 (MQ=60)
                                                                                           TTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAG                                                           >  SRR19973063.2258450/1‑150 (MQ=60)
                                                                                             TCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCG                                                         >  SRR19973063.4528468/1‑150 (MQ=60)
                                                                                             TCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCG                                                         <  SRR19973063.575696/150‑1 (MQ=60)
                                                                                                TGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTAT                                                      >  SRR19973063.4249757/1‑150 (MQ=60)
                                                                                                  TGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAA                                                    >  SRR19973063.4271823/1‑150 (MQ=60)
                                                                                                  TGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAA                                                    >  SRR19973063.4308019/1‑150 (MQ=60)
                                                                                                  TGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAA                                                    >  SRR19973063.1284038/1‑150 (MQ=60)
                                                                                                   GAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAC                                                   <  SRR19973063.3266206/150‑1 (MQ=60)
                                                                                                    AAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACC                                                  >  SRR19973063.3057556/1‑150 (MQ=60)
                                                                                                    AAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACC                                                  >  SRR19973063.4675348/1‑150 (MQ=60)
                                                                                                     AACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCC                                                 >  SRR19973063.2322187/1‑150 (MQ=60)
                                                                                                      ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                <  SRR19973063.4807975/150‑1 (MQ=60)
                                                                                                      ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                <  SRR19973063.5055730/150‑1 (MQ=60)
                                                                                                      ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                <  SRR19973063.2659344/150‑1 (MQ=60)
                                                                                                          CTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTG                                            <  SRR19973063.2403251/150‑1 (MQ=60)
                                                                                                          CTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCGTCTG                                            >  SRR19973063.4926544/1‑150 (MQ=60)
                                                                                                            GCGCGATTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCA                                          <  SRR19973063.3335284/150‑1 (MQ=60)
                                                                                                             CGCGAGAGTGTGGCGTAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAT                                         <  SRR19973063.364204/150‑1 (MQ=60)
                                                                                                               CGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAA                                       >  SRR19973063.3664855/1‑150 (MQ=60)
                                                                                                                 AGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATG                                     >  SRR19973063.1009204/1‑150 (MQ=60)
                                                                                                                 AGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATG                                     >  SRR19973063.2110251/1‑150 (MQ=60)
                                                                                                                 AGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATCAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATG                                     <  SRR19973063.3173945/150‑1 (MQ=60)
                                                                                                                   AGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCC                                   >  SRR19973063.3376515/1‑150 (MQ=60)
                                                                                                                     TGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCA                                 >  SRR19973063.4033162/1‑150 (MQ=60)
                                                                                                                      GTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAG                                >  SRR19973063.3854824/1‑150 (MQ=60)
                                                                                                                       TGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGT                               <  SRR19973063.4719558/150‑1 (MQ=60)
                                                                                                                          CTGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGTACTTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACG                            <  SRR19973063.3524253/150‑1 (MQ=60)
                                                                                                                           GGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGT                           >  SRR19973063.3150974/1‑150 (MQ=60)
                                                                                                                               TAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACC                       >  SRR19973063.1878183/1‑150 (MQ=60)
                                                                                                                               TAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACC                       >  SRR19973063.4591317/1‑150 (MQ=60)
                                                                                                                                  CGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGG                    >  SRR19973063.3739889/1‑150 (MQ=60)
                                                                                                                                  CGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGG                    >  SRR19973063.3671248/1‑150 (MQ=60)
                                                                                                                                     AAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGC                 >  SRR19973063.2807094/1‑150 (MQ=60)
                                                                                                                                     AAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGC                 >  SRR19973063.3530689/1‑150 (MQ=60)
                                                                                                                                      AACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCA                >  SRR19973063.4556847/1‑150 (MQ=60)
                                                                                                                                        CGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAAC              >  SRR19973063.4440021/1‑150 (MQ=60)
                                                                                                                                           CCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTC           <  SRR19973063.4684917/150‑1 (MQ=60)
                                                                                                                                           CCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTC           >  SRR19973063.98529/1‑150 (MQ=60)
                                                                                                                                             CGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCAT         <  SRR19973063.3836402/150‑1 (MQ=60)
                                                                                                                                              GGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATC        <  SRR19973063.4283394/150‑1 (MQ=60)
                                                                                                                                                CATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAG      >  SRR19973063.4485381/1‑150 (MQ=60)
                                                                                                                                                 ATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGA     >  SRR19973063.1312085/1‑150 (MQ=60)
                                                                                                                                                   TAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATT   >  SRR19973063.4040281/1‑150 (MQ=60)
                                                                                                                                                    AGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  SRR19973063.3638667/1‑150 (MQ=60)
                                                                                                                                                    AGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  SRR19973063.3250900/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  CP009273/2743290‑2743587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑