Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,743,438 C→A 100% intergenic (+19/+36) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,743,4380CA99.2% 789.7 / ‑2.1 243intergenic (+19/+36)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (138/103);  total (139/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

CTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAG  >  CP009273/2743295‑2743583
                                                                                                                                               |                                                                                                                                                 
cTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACTATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                              <  2:896658/149‑1 (MQ=255)
cTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACTGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                              <  2:896202/149‑1 (MQ=255)
 tGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATTTTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACTAAAACGGCCCGGCATTAGCATGc                                                                                                                                             <  2:3464664/149‑1 (MQ=255)
       aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATAGGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCTAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGcc                                                                                                                                       <  2:3562567/149‑1 (MQ=255)
        ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                      >  1:452617/1‑149 (MQ=255)
        ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                      >  2:3423883/1‑149 (MQ=255)
        ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                      >  2:4113479/1‑149 (MQ=255)
         gCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGt                                                                                                                                     >  2:2691293/1‑149 (MQ=255)
              cgcgTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAatt                                                                                                                                >  2:1404207/1‑149 (MQ=255)
               gcgtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAatta                                                                                                                               >  2:3467456/1‑149 (MQ=255)
               gcgtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAatta                                                                                                                               <  2:2280074/149‑1 (MQ=255)
                  tgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAAttatta                                                                                                                            >  1:264628/1‑149 (MQ=255)
                     gtATTGGCGAGTACGATATCTATATTCTCGACGTACAGGACAATATGTTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCGGCGCGAGTTTGTGGCGGAGAAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGa                                                                                                                         <  2:770465/149‑1 (MQ=255)
                     gtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGa                                                                                                                         <  2:618335/149‑1 (MQ=255)
                      tATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGaa                                                                                                                        >  1:3870387/1‑149 (MQ=255)
                      tATTGGCGAGTACGATATCGATATTCTCCACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGaa                                                                                                                        <  2:2456116/149‑1 (MQ=255)
                       aTTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGaaa                                                                                                                       >  1:1867920/1‑149 (MQ=255)
                       aTTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGaaa                                                                                                                       >  1:2727051/1‑149 (MQ=255)
                          ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCa                                                                                                                    >  2:2478157/1‑149 (MQ=255)
                            cGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGa                                                                                                                  <  2:934380/149‑1 (MQ=255)
                            cGAGTACGATATCGATATTCGCGCCGTACAGGAAATTATGATTAAGCAGTAAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGCGTGTGGCGGAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGa                                                                                                                  <  1:873511/149‑1 (MQ=255)
                              aGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGaaa                                                                                                                >  1:1082317/1‑149 (MQ=255)
                               gTACGTTATCGATATTCTCGACTTCAAGCAAAATTTGATTACGATGTTAAAAGTTTTTCCTGTCAACCCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAt                                                                                                               <  1:1159501/149‑1 (MQ=255)
                                tACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAtt                                                                                                              >  2:2580900/1‑149 (MQ=255)
                                   gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                           >  2:3189882/1‑149 (MQ=255)
                                    atatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAATCAGAAATTAAGc                                                                                                          >  1:2249100/1‑149 (MQ=255)
                                     tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                                         >  2:4012214/1‑149 (MQ=255)
                                       tcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCttt                                                                                                       >  2:3511414/1‑149 (MQ=255)
                                        ctatttgctcGACGTACAGCACAATATGATTAAGCTGGTAAAAGTTTTGCCGGTGAACCCGCTGCGCGAGCGGGTGGGGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtttt                                                                                                      <  1:3518016/142‑1 (MQ=255)
                                         gatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                     >  1:1048191/1‑149 (MQ=255)
                                           tatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                   >  1:3431503/1‑149 (MQ=255)
                                           tagtctcGACGTACAGGACAATATGATTACGCAGGTAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                   <  2:60047/146‑1 (MQ=255)
                                           taagctcGACGCACAGGACAATAGGATTACGCAGGTAAAAGTTTTGCCTGTGACACCGCGGCGCGAGAGGGTGGCGGAGTAACCAACACGGCCCGGCATTAGCAGGCAGGGCCGTGAATGATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                   <  2:3984745/145‑1 (MQ=255)
                                            atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCtt                                                                                                  >  2:3083379/1‑149 (MQ=255)
                                             ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                 <  1:2019107/149‑1 (MQ=255)
                                             ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                 >  1:3780571/1‑149 (MQ=255)
                                               ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCg                                                                                               >  1:513440/1‑149 (MQ=255)
                                                tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                              <  2:2408612/149‑1 (MQ=255)
                                                 ggACGTACAGGACATTTTGATTAAGCTGTTAAAATTTTTTCCTGTTAAACCGCTGCGCGAGTGTGTGGCGTTGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCt                                                                                             <  2:1059384/148‑1 (MQ=255)
                                                  gACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                                                                                            >  1:477254/1‑149 (MQ=255)
                                                   aCGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAc                                                                                           >  2:1932944/1‑149 (MQ=255)
                                                         agtaCAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                     >  2:156753/4‑149 (MQ=255)
                                                          ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAcc                                                                                    >  1:1782597/1‑149 (MQ=255)
                                                           gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATTCAGGGCCGTGAATTATTAAGAAAGCAGAAATTAAGCTTTTGCTTACGCTACAGTaaaaa                                                                                   >  1:1223624/1‑146 (MQ=255)
                                                           gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCa                                                                                   >  2:2494901/1‑149 (MQ=255)
                                                            aCAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                                  >  1:2383117/1‑149 (MQ=255)
                                                              aaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg                                                                                >  2:2577504/1‑149 (MQ=255)
                                                              aaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg                                                                                >  1:1888079/1‑149 (MQ=255)
                                                               ttatGATTAAGCAGTTAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                               <  1:2869017/148‑1 (MQ=255)
                                                               atatGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                               <  1:2818905/149‑1 (MQ=255)
                                                               atatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                               >  1:1199191/1‑149 (MQ=255)
                                                                tatGGTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                              >  2:3645636/1‑149 (MQ=255)
                                                                     ttAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                         >  2:1605757/1‑149 (MQ=255)
                                                                     ttAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                         >  1:2115490/1‑149 (MQ=255)
                                                                       aaGCTGTTAAAAGTTTTTCCTGTGAAACCGGTGCGCTAGTGTGTGGCGGAGTAACAAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGaa                                                                       <  1:3109028/149‑1 (MQ=255)
                                                                       aaGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGaa                                                                       <  1:1713572/149‑1 (MQ=255)
                                                                        aGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAAt                                                                      >  2:3718161/1‑149 (MQ=255)
                                                                         gCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAATCGGCCCGGCATGAGCATGAAGGTCAGTGAATTATTACGAAATCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                     <  2:665563/149‑1 (MQ=255)
                                                                         gCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGACTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                     <  2:2996237/149‑1 (MQ=255)
                                                                           aGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTCCAGTAACCATCGCCGCACGAAGCGt                                                                   >  2:2774816/1‑149 (MQ=255)
                                                                           aGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                                   <  2:1840414/149‑1 (MQ=255)
                                                                           aGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                                   >  2:323918/1‑149 (MQ=255)
                                                                            ggTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTa                                                                  <  2:907320/149‑1 (MQ=255)
                                                                            ggTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTa                                                                  >  1:3991458/1‑149 (MQ=255)
                                                                              tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGAGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACCGTAACCCTCGCCGCACGAATCGTACg                                                                >  2:1981651/1‑149 (MQ=255)
                                                                                aaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                                              >  2:3289781/1‑149 (MQ=255)
                                                                                          cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                    >  2:2313113/1‑149 (MQ=255)
                                                                                           tgtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                   <  1:3578673/149‑1 (MQ=255)
                                                                                            gtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtat                                                  >  2:2797809/1‑149 (MQ=255)
                                                                                            gtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtat                                                  >  2:468259/1‑149 (MQ=255)
                                                                                             tgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                 <  1:316205/149‑1 (MQ=255)
                                                                                              gAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                >  1:4087766/1‑149 (MQ=255)
                                                                                                aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAcc                                              >  2:765049/1‑149 (MQ=255)
                                                                                                 aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                             <  1:3067536/149‑1 (MQ=255)
                                                                                                 aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                             >  2:3927770/1‑149 (MQ=255)
                                                                                                 aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                             >  1:1002978/1‑149 (MQ=255)
                                                                                                  ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGACTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                            >  1:2855469/1‑149 (MQ=255)
                                                                                                  ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                            >  1:4110817/1‑149 (MQ=255)
                                                                                                  ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                            >  2:2049713/1‑149 (MQ=255)
                                                                                                   cGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCtt                                           <  2:2920812/149‑1 (MQ=255)
                                                                                                    gCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                          <  1:1556533/149‑1 (MQ=255)
                                                                                                       gcgcGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGc                                       >  2:3339367/1‑149 (MQ=255)
                                                                                                            agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAAt                                  >  1:1950240/1‑149 (MQ=255)
                                                                                                            agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCATTCGCAACAGTAACCATCGCCGCCCGAATCGTACGACCATTCAGCGGATAACCCTTCTGCATAAt                                  >  1:3709537/1‑149 (MQ=255)
                                                                                                             gagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                 >  1:1861960/1‑149 (MQ=255)
                                                                                                             gagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                 >  2:1676235/1‑149 (MQ=255)
                                                                                                              agTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGc                                <  1:1663592/149‑1 (MQ=255)
                                                                                                               gtgtGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGcc                               >  1:3351715/1‑149 (MQ=255)
                                                                                                                 gtgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa                             >  1:2535820/1‑149 (MQ=255)
                                                                                                                       gAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                       <  2:3797401/149‑1 (MQ=255)
                                                                                                                       gAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                       <  2:1219012/149‑1 (MQ=255)
                                                                                                                       gAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                       <  2:3780571/149‑1 (MQ=255)
                                                                                                                       gAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                       <  1:169384/149‑1 (MQ=255)
                                                                                                                        aGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt                      <  1:1932944/149‑1 (MQ=255)
                                                                                                                         gTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTa                     >  1:2414587/1‑149 (MQ=255)
                                                                                                                          tAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACTACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                    <  2:1795893/149‑1 (MQ=255)
                                                                                                                            aCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                  <  1:615960/149‑1 (MQ=255)
                                                                                                                             cGAAAACGGCCCGGCATTAGCATGCAGGGGCGTGTATTATTACGAAAGCAGTAATTTAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTg                 <  1:1612822/149‑1 (MQ=255)
                                                                                                                               aaaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgc               <  1:3669121/149‑1 (MQ=255)
                                                                                                                                   cGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCaa           <  2:1048212/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAG  >  CP009273/2743295‑2743583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  CP009273/2743297‑2743587
                                                                                                                                             |                                                                                                                                                     
ggcgccggcacccgggggcgtttgggctAGTACGATATCGATATTCTCGCCGTACAGCACAATTTTTTTAAGCTGGTAAAAGTTTGCCGGGTGAACCCGCTGCGCGTGTGTGGGGCGGAGCAACCAACACGGCCCGGCATTAGCATGCAG                                                                                                                                               <  SRR19973068.475369/122‑1 (MQ=16)
  CGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGTTAAATTTTTTTCCTGTTAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGG                                                                                                                                             <  SRR19973068.574775/150‑1 (MQ=60)
     AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCG                                                                                                                                          >  SRR19973068.455823/1‑150 (MQ=60)
     AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCG                                                                                                                                          >  SRR19973068.455803/1‑150 (MQ=60)
        CACCCGCGTGCGTTTTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGGGTGGCGGAGTACCGAACACGGCCCGGCATTAGCATGCAGGGCCGTGA                                                                                                                                       <  SRR19973068.2262127/150‑1 (MQ=60)
        CACCCGCGTGCGTATTGGCGAGTACTATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGAAAATGTTTTTCCTGTCAAACCGCGGCGCGAGCGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGA                                                                                                                                       <  SRR19973068.3603873/150‑1 (MQ=60)
        CACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGCAAAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGAAACCAACACGGCCCGGCATTAGCATGCAGGGCCGTGA                                                                                                                                       <  SRR19973068.3603881/150‑1 (MQ=60)
         ACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAA                                                                                                                                      >  SRR19973068.183958/1‑150 (MQ=60)
           CCGGGTGCGTCTGGGCGAGTACTAAATCGATTTTCTCGACGTACAGGACAATATTATTAAGCAGGAAAATGTTTTTCCTGTCAAACCGGGGCGCGAGTGGGGGGCGCATTAACCAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATT                                                                                                                                    <  SRR19973068.2439049/150‑1 (MQ=41)
           CCGGGTGCGTATGGGCGAGAACTATATCTATATTCTCGACTTACCGTACATTATGATTAAGCAGTAAAATGTTTTTCCTGTGAACCCGCGGCGCGTGTGTGTGGCGTAGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATT                                                                                                                                    <  SRR19973068.18606/150‑1 (MQ=39)
            cgggtggtttTTGGCGAGTCCGATATCGTTATTCTCGACGTACAGTAAAATATGTTAACCCAGGTAAATGTTTTTCCTGTGAAACCGCTGCGCGTGTGGGTGGGGGTTTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTA                                                                                                                                   <  SRR19973068.2822926/140‑1 (MQ=56)
            CGCGTGCGTTTTGGCGAGTACTATATCTATATTCTCGACTTACCGGACAATATGTTTAAGCAGTTAAAATTTTTTCGTGTGAAACCGCTGCGCGAGTGTGGGGCGGTGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTA                                                                                                                                   <  SRR19973068.2822858/150‑1 (MQ=60)
              CGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATT                                                                                                                                 >  SRR19973068.1223588/1‑150 (MQ=60)
               ggggGTATTGGCGAGTACGATATCGATTTTCACGACGTACAGGAAAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGATAGTGTGGCGTAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTA                                                                                                                                <  SRR19973068.765889/146‑1 (MQ=60)
               GTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTA                                                                                                                                >  SRR19973068.266504/1‑150 (MQ=60)
               GTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTA                                                                                                                                >  SRR19973068.260252/1‑150 (MQ=60)
                tggtttttggggtgaactATATCGATATGCTCGACGTACAGGAAAATTTTATTAAGCTGTTAAATTTTTTTCCTGTCAACCCGCTGCGCGTGTGTGTGGGGTATTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTAC                                                                                                                               <  SRR19973068.355580/132‑1 (MQ=37)
                 GCGTATTGGCGATTACGATATCGATAGTCTCTACTTACAGTACAATATGTTTAAGCAGTTAAAATTTTTTCCTGTGAAACCGGTGCGCGTGTGTGGGGCGGAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACG                                                                                                                              <  SRR19973068.3523442/150‑1 (MQ=60)
                  CGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGACCGGCATTAGAAAGCAGGGCCGTGAATAATTACTA                                                                                                                             >  SRR19973068.412286/1‑150 (MQ=60)
                   GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973068.3893523/1‑150 (MQ=60)
                   GTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAA                                                                                                                            >  SRR19973068.3893753/1‑150 (MQ=60)
                    TATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAA                                                                                                                           >  SRR19973068.2743885/1‑150 (MQ=60)
                    TATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAA                                                                                                                           >  SRR19973068.1880274/1‑150 (MQ=60)
                    TATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAA                                                                                                                           >  SRR19973068.1880250/1‑150 (MQ=60)
                       TGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCA                                                                                                                        >  SRR19973068.2738721/1‑150 (MQ=60)
                       TGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGGGAATTATTACGAAAGCA                                                                                                                        >  SRR19973068.2030017/1‑150 (MQ=60)
                          CGAGTACGATATCGATATTCGCGCCGTACAGGAAATTATGATTAAGCAGTAAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGCGTGTGGCGGAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAA                                                                                                                     <  SRR19973068.879441/150‑1 (MQ=60)
                           GAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAA                                                                                                                    >  SRR19973068.1089492/1‑150 (MQ=60)
                            AGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGAAGAAAT                                                                                                                   >  SRR19973068.1710741/1‑150 (MQ=60)
                             gtacgttatcgatattctcgacttcaagcaaaatttgattacgatgtTAAAAGTTTTTCCTGTCAACCCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATT                                                                                                                  <  SRR19973068.1167262/103‑1 (MQ=60)
                             TTACGATATCGATATTCTCGACGTACAGGACAATATTATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATT                                                                                                                  <  SRR19973068.2388507/150‑1 (MQ=60)
                                 GATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGC                                                                                                              >  SRR19973068.1139819/1‑150 (MQ=60)
                                 GATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAATCAGAAATTAAGC                                                                                                              >  SRR19973068.2263751/1‑150 (MQ=60)
                                  ATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCT                                                                                                             >  SRR19973068.2196575/1‑150 (MQ=60)
                                      ctatttgCTCGACGTACAGCACAATATGATTAAGCTGGTAAAAGTTTTGCCGGTGAACCCGCTGCGCGAGCGGGTGGGGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                         <  SRR19973068.3539409/143‑1 (MQ=60)
                                      CGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                         >  SRR19973068.1054957/1‑150 (MQ=60)
                                      CGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                         >  SRR19973068.1054965/1‑150 (MQ=60)
                                      CGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                         >  SRR19973068.1054988/1‑150 (MQ=60)
                                       GATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTTAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGC                                                                                                        <  SRR19973068.1636291/150‑1 (MQ=60)
                                       GATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGC                                                                                                        >  SRR19973068.379168/1‑150 (MQ=60)
                                       GATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGC                                                                                                        >  SRR19973068.770977/1‑150 (MQ=60)
                                        ATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCT                                                                                                       >  SRR19973068.3452166/1‑150 (MQ=60)
                                          ATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTT                                                                                                     >  SRR19973068.1592911/1‑150 (MQ=60)
                                          ATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTT                                                                                                     >  SRR19973068.3803135/1‑150 (MQ=60)
                                          ATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTT                                                                                                     >  SRR19973068.3803129/1‑150 (MQ=60)
                                           TTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTACCCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTC                                                                                                    <  SRR19973068.2032315/150‑1 (MQ=60)
                                           TTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTC                                                                                                    <  SRR19973068.2032357/150‑1 (MQ=60)
                                            TCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCG                                                                                                   >  SRR19973068.517005/1‑150 (MQ=60)
                                            TCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCG                                                                                                   >  SRR19973068.517088/1‑150 (MQ=60)
                                               CGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTA                                                                                                >  SRR19973068.480622/1‑150 (MQ=60)
                                                  CGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAG                                                                                             >  SRR19973068.1227124/1‑150 (MQ=60)
                                                       AGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACC                                                                                        >  SRR19973068.2496103/1‑150 (MQ=60)
                                                       AGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACC                                                                                        >  SRR19973068.1794203/1‑150 (MQ=60)
                                                       AGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACC                                                                                        >  SRR19973068.1794171/1‑150 (MQ=60)
                                                        GGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATTCAGGGCCGTGAATTATTAAGAAAGCAGAAATTAAGCTTTTGCTTACGCTACAGTAAaaa                                                                                       >  SRR19973068.1231761/1‑147 (MQ=60)
                                                         GACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAT                                                                                      >  SRR19973068.2398320/1‑150 (MQ=60)
                                                           cattttgtttatgcagtTAAAATTTTTGCCTGTGAAACCGGGGCGGGTGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCG                                                                                    <  SRR19973068.2963215/133‑1 (MQ=60)
                                                           CAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCG                                                                                    >  SRR19973068.1900622/1‑150 (MQ=60)
                                                            AATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGC                                                                                   >  SRR19973068.1206838/1‑150 (MQ=60)
                                                            AATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGC                                                                                   >  SRR19973068.1207189/1‑150 (MQ=60)
                                                             TTATGATTAAGCAGTTAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCC                                                                                  <  SRR19973068.2886892/150‑1 (MQ=60)
                                                             ATATTATTAAGCAGTTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCC                                                                                  <  SRR19973068.288214/150‑1 (MQ=60)
                                                             ATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCC                                                                                  <  SRR19973068.2836440/150‑1 (MQ=60)
                                                               ATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTTCTTTCGCTACAGTAACCATCGCCGC                                                                                >  SRR19973068.1522333/1‑150 (MQ=60)
                                                                  ATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACG                                                                             >  SRR19973068.2129299/1‑150 (MQ=60)
                                                                  ATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACG                                                                             >  SRR19973068.1603283/1‑150 (MQ=60)
                                                                  ATTAAGAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACG                                                                             <  SRR19973068.3967173/150‑1 (MQ=60)
                                                                     AAGCTGTTAAAAGTTTTTCCTGTGAAACCGGTGCGCTAGTGTGTGGCGGAGTAACAAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAAT                                                                          <  SRR19973068.3127999/150‑1 (MQ=60)
                                                                     AAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAAT                                                                          <  SRR19973068.1724494/150‑1 (MQ=60)
                                                                     AAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAAT                                                                          <  SRR19973068.1724441/150‑1 (MQ=60)
                                                                       GCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCG                                                                        >  SRR19973068.3712607/1‑150 (MQ=60)
                                                                       GCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCG                                                                        >  SRR19973068.495652/1‑150 (MQ=60)
                                                                         AGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTA                                                                      >  SRR19973068.4015583/1‑150 (MQ=60)
                                                                           GTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACG                                                                    >  SRR19973068.456823/1‑150 (MQ=60)
                                                                             AAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAC                                                                  >  SRR19973068.2863180/1‑150 (MQ=60)
                                                                               AAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCA                                                                >  SRR19973068.1675813/1‑150 (MQ=60)
                                                                               AAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCA                                                                >  SRR19973068.3364197/1‑150 (MQ=60)
                                                                               AAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCA                                                                >  SRR19973068.3363424/1‑150 (MQ=60)
                                                                                AGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAT                                                               >  SRR19973068.3450357/1‑150 (MQ=60)
                                                                                      TCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGGATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCG                                                         >  SRR19973068.412989/1‑150 (MQ=60)
                                                                                         TGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTAT                                                      >  SRR19973068.1899615/1‑150 (MQ=60)
                                                                                         TGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTAT                                                      >  SRR19973068.1899683/1‑150 (MQ=60)
                                                                                         TGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTAT                                                      <  SRR19973068.3599285/150‑1 (MQ=60)
                                                                                         TGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTAT                                                      <  SRR19973068.3600411/150‑1 (MQ=60)
                                                                                           TGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAA                                                    <  SRR19973068.318267/150‑1 (MQ=60)
                                                                                           TGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAA                                                    >  SRR19973068.4112277/1‑150 (MQ=60)
                                                                                             AAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACC                                                  >  SRR19973068.1827017/1‑150 (MQ=60)
                                                                                             AAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACC                                                  >  SRR19973068.2992808/1‑150 (MQ=60)
                                                                                              AACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCC                                                 >  SRR19973068.1009531/1‑150 (MQ=60)
                                                                                               ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                <  SRR19973068.3086277/150‑1 (MQ=60)
                                                                                               ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGACTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                >  SRR19973068.2873259/1‑150 (MQ=60)
                                                                                               ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                >  SRR19973068.4135413/1‑150 (MQ=60)
                                                                                               ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                >  SRR19973068.4135406/1‑150 (MQ=60)
                                                                                               ACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATAGCCGCACGAATCGTACGACCATTCAGCGTATAACCCT                                                >  SRR19973068.2873254/1‑150 (MQ=60)
                                                                                                  GCTGCGCGAGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCT                                             <  SRR19973068.1566778/150‑1 (MQ=60)
                                                                                                  GCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCT                                             <  SRR19973068.2663758/150‑1 (MQ=60)
                                                                                                   CTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTG                                            <  SRR19973068.2402948/150‑1 (MQ=60)
                                                                                                   CTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTG                                            <  SRR19973068.2402945/150‑1 (MQ=60)
                                                                                                   CTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTG                                            >  SRR19973068.2110358/1‑150 (MQ=60)
                                                                                                     GCGCGAGAGTGTGGCGGAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCA                                          <  SRR19973068.1089828/150‑1 (MQ=60)
                                                                                                         GAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAAT                                      >  SRR19973068.1963183/1‑150 (MQ=60)
                                                                                                         GAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCATTCGCAACAGTAACCATCGCCGCCCGAATCGTACGACCATTCAGCGGATAACCCTTCTGCATAAT                                      >  SRR19973068.3731847/1‑150 (MQ=60)
                                                                                                          AGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATG                                     >  SRR19973068.1874269/1‑150 (MQ=60)
                                                                                                            AGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCC                                   >  SRR19973068.3372127/1‑150 (MQ=60)
                                                                                                            AGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCC                                   <  SRR19973068.282892/150‑1 (MQ=60)
                                                                                                            AGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCC                                   <  SRR19973068.1674347/150‑1 (MQ=60)
                                                                                                              TGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCA                                 >  SRR19973068.2551935/1‑150 (MQ=60)
                                                                                                                     GTGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTT                          <  SRR19973068.170766/150‑1 (MQ=60)
                                                                                                                      AGTACCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTA                         <  SRR19973068.1945792/150‑1 (MQ=60)
                                                                                                                      AGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTA                         >  SRR19973068.2429890/1‑150 (MQ=60)
                                                                                                                      AGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTA                         <  SRR19973068.1945853/150‑1 (MQ=60)
                                                                                                                         AACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCT                      <  SRR19973068.3128835/150‑1 (MQ=60)
                                                                                                                          ACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTG                     <  SRR19973068.620070/150‑1 (MQ=60)
                                                                                                                           CGAAAACGGCCCGGCATTAGCATGCAGGGGCGTGTATTATTACGAAAGCAGTAATTTAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGG                    <  SRR19973068.1623375/150‑1 (MQ=60)
                                                                                                                           CGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGG                    >  SRR19973068.2896655/1‑150 (MQ=60)
                                                                                                                             AAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCG                  <  SRR19973068.3088469/150‑1 (MQ=60)
                                                                                                                             AAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCG                  <  SRR19973068.3691296/150‑1 (MQ=60)
                                                                                                                                      CGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCAT         <  SRR19973068.3488467/150‑1 (MQ=60)
                                                                                                                                        GCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCA       <  SRR19973068.1926605/150‑1 (MQ=60)
                                                                                                                                         CATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAG      <  SRR19973068.4036447/150‑1 (MQ=60)
                                                                                                                                         CATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAG      <  SRR19973068.4036319/150‑1 (MQ=60)
                                                                                                                                             AGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  SRR19973068.600880/1‑150 (MQ=60)
                                                                                                                                             AGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  SRR19973068.600901/1‑150 (MQ=60)
                                                                                                                                             |                                                                                                                                                     
GTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTC  >  CP009273/2743297‑2743587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑