Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,743,438 C→A 100% intergenic (+19/+36) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,743,4380CA99.4% 566.7 / ‑4.2 174intergenic (+19/+36)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (79/94);  total (80/94)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

ATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCA  >  CP009273/2743291‑2743582
                                                                                                                                                   |                                                                                                                                                
aTCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTc                                                                                                                                                  >  2:1734550/1‑148 (MQ=255)
  cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGc                                                                                                                                                <  2:2310793/148‑1 (MQ=255)
  cccGGTCGCAGGCACCCGCGTGCGTATGGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGc                                                                                                                                                <  2:3450557/148‑1 (MQ=255)
    cTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCAt                                                                                                                                              >  1:586370/1‑148 (MQ=255)
     tGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAATAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                             >  2:2477568/1‑148 (MQ=255)
     tGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATg                                                                                                                                             >  1:360291/1‑148 (MQ=255)
      gTCGCTGGCACCCGCGTGCTTATTGGCGATTACTATATCGATTTTCTCGCCGTACAGGAAAATATTATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGc                                                                                                                                            <  1:2510676/148‑1 (MQ=255)
      gTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGATTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGc                                                                                                                                            >  1:2840684/1‑148 (MQ=255)
            ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGcc                                                                                                                                      >  1:1644399/1‑148 (MQ=255)
             gCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCg                                                                                                                                     >  1:3317706/1‑148 (MQ=255)
              cACCCGCGTGCGTATTGGCGAGTACGATAACGATATTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGAGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGt                                                                                                                                    <  2:1495419/148‑1 (MQ=255)
              cACCCGCGTGCGTATGGGCTAGTACGATATCGAAATTCTCGACGTACCGTACAATATGATTACCCAGGAAAAAGTTTTACCGGTCAAACCGCTGCGCGAGAGTGTGGCGTAGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGt                                                                                                                                    <  2:3237855/148‑1 (MQ=255)
                cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTCAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                  <  2:197172/148‑1 (MQ=255)
                cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCTGGTAAAAGTTTTGCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                  <  2:743038/148‑1 (MQ=255)
                cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGa                                                                                                                                  <  1:2304451/148‑1 (MQ=255)
                     gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattat                                                                                                                             <  2:177419/148‑1 (MQ=255)
                      tgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAattatt                                                                                                                            >  1:3267385/1‑148 (MQ=255)
                          tATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGa                                                                                                                        >  1:798349/1‑148 (MQ=255)
                                   gTACTATATCGATATTCTCGACGTACTGGACAATATTATTAAGCAGGTAAATGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGGGGTGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGaaa                                                                                                               <  1:3004465/148‑1 (MQ=255)
                                    tACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGATAGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAt                                                                                                              <  2:1528536/148‑1 (MQ=255)
                                     aCGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAtt                                                                                                             >  2:2363343/1‑148 (MQ=255)
                                        atatctatatTCTCTACGTACAGGAAAATTTTATTAAGCATTTAAAAGTTTTTCCTGTTAAACCGCTGCGCGAGTGTGTGGCGTAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                          <  1:213316/148‑1 (MQ=255)
                                        atatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                          <  1:823586/148‑1 (MQ=255)
                                         tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAACTTACGc                                                                                                         >  2:2257012/1‑148 (MQ=255)
                                         tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAACTTAAGc                                                                                                         >  2:1608090/1‑148 (MQ=255)
                                         tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGc                                                                                                         <  2:3372313/148‑1 (MQ=255)
                                          atcgatatTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                                        <  1:2542694/148‑1 (MQ=255)
                                            cgatttTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCttt                                                                                                      <  2:681319/148‑1 (MQ=255)
                                            cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCttt                                                                                                      <  1:1401346/148‑1 (MQ=255)
                                              atatTCTCGACGTACAGGACAATATTATTAAGAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                    <  1:2463014/148‑1 (MQ=255)
                                              atatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                    >  2:2837457/1‑148 (MQ=255)
                                                atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                  >  2:1527755/1‑148 (MQ=255)
                                                    tcGACGTACAGTAAAATATGATTAAGCAGTTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGAAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCg                                                                                              <  1:1574564/148‑1 (MQ=255)
                                                     cGACGTACAGGACAATATGATTAAGCAGGTAAATGTTGTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                             <  1:3399745/148‑1 (MQ=255)
                                                     cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTGTCCTGTGAACCCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                             <  1:3398504/148‑1 (MQ=255)
                                                         gTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACa                                                                                         <  2:2459885/148‑1 (MQ=255)
                                                             aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTaa                                                                                     >  2:1511245/1‑148 (MQ=255)
                                                              ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                    >  2:1098151/1‑148 (MQ=255)
                                                              ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                    <  1:3193579/148‑1 (MQ=255)
                                                               gACAATATGATTAAGCAGGTAAAAGTTTTTCCGGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAcc                                                                                   <  2:1729507/148‑1 (MQ=255)
                                                                    tatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                              <  2:292460/148‑1 (MQ=255)
                                                                     atGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                             >  2:2494214/1‑148 (MQ=255)
                                                                     atGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                             >  2:2201083/1‑148 (MQ=255)
                                                                       gATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgccgc                                                                           >  2:1386625/1‑148 (MQ=255)
                                                                        aTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCa                                                                          >  2:1950569/1‑148 (MQ=255)
                                                                         ttAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCAc                                                                         >  2:723336/1‑148 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                        >  2:2339587/1‑148 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                        >  2:2339551/1‑148 (MQ=255)
                                                                          tAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTAAGAAAGCAGAAATTAATCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                        >  1:2402798/1‑148 (MQ=255)
                                                                           aaGCATGTAACAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                       <  2:1221131/148‑1 (MQ=255)
                                                                           aaGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGTGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                       <  2:1221716/148‑1 (MQ=255)
                                                                           aaGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGa                                                                       <  2:3154961/148‑1 (MQ=255)
                                                                              ctggTAAAAGTTTGTCCTGTGAACCCGCTGCGCGAGAGGGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                    <  1:2459901/146‑1 (MQ=255)
                                                                              cAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                    >  1:139630/1‑148 (MQ=255)
                                                                              cAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATc                                                                    >  1:2075594/1‑148 (MQ=255)
                                                                               agttAAATGTTTTTCCTGTGAAACCGCTGCGCGTGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCg                                                                   <  1:1623188/145‑1 (MQ=255)
                                                                                ggTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                                  >  1:1865225/1‑148 (MQ=255)
                                                                                 gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGtc                                                                 >  2:3239464/1‑147 (MQ=255)
                                                                                 gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTa                                                                 >  2:1106242/1‑148 (MQ=255)
                                                                                     aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                                             <  2:3293085/148‑1 (MQ=255)
                                                                                      aGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAcc                                                            >  1:1042205/1‑148 (MQ=255)
                                                                                        tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAt                                                          >  2:1598538/1‑148 (MQ=255)
                                                                                        tttttCCGGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAt                                                          <  2:1300551/148‑1 (MQ=255)
                                                                                         ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAtt                                                         <  1:3061764/148‑1 (MQ=255)
                                                                                               tgtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                   >  2:2638593/1‑148 (MQ=255)
                                                                                                gtgAACCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                  <  2:979035/148‑1 (MQ=255)
                                                                                                  taacccGCTGCGCGAGTGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                <  1:1224522/144‑1 (MQ=255)
                                                                                                  gacaCCGCTGCGCGAGAGTGTGGCGTAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtata                                                <  1:1224583/145‑1 (MQ=255)
                                                                                                     aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAcc                                             >  2:3273869/1‑148 (MQ=255)
                                                                                                      ccGCTGCGCTAGAGTGGGGCGGTGTATATAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                            <  1:2192739/148‑1 (MQ=255)
                                                                                                      ccGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                            >  1:1341044/1‑148 (MQ=255)
                                                                                                       cGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                           <  2:586370/148‑1 (MQ=255)
                                                                                                         cTGCGCGTGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                         <  1:3098191/148‑1 (MQ=255)
                                                                                                         cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                         <  2:1757220/148‑1 (MQ=255)
                                                                                                         cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                         >  1:1129968/1‑148 (MQ=255)
                                                                                                            cgcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGc                                      <  1:1827981/148‑1 (MQ=255)
                                                                                                              cgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                    >  2:1116508/1‑148 (MQ=255)
                                                                                                                agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATaa                                  <  2:3317706/148‑1 (MQ=255)
                                                                                                                   gtgtGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATgt                               <  1:886147/148‑2 (MQ=255)
                                                                                                                    tgtgGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGcc                              >  2:1039713/1‑148 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                         <  1:2477568/148‑1 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                         <  2:3121325/148‑1 (MQ=255)
                                                                                                                         cGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                         <  2:3121415/148‑1 (MQ=255)
                                                                                                                            aGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                      <  1:2704334/148‑1 (MQ=255)
                                                                                                                            aGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                      >  2:1875670/1‑148 (MQ=255)
                                                                                                                             gTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt                     <  1:950670/148‑1 (MQ=255)
                                                                                                                               acagAAAACGGACCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGTATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                   <  2:516948/145‑1 (MQ=255)
                                                                                                                                ccGACAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAcc                  <  1:949857/147‑1 (MQ=255)
                                                                                                                                aCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGTCCAGTACGTTAcc                  <  1:1343612/148‑1 (MQ=255)
                                                                                                                                aCGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAca                  >  2:3018597/1‑147 (MQ=255)
                                                                                                                                  gAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTg                <  1:799904/148‑1 (MQ=255)
                                                                                                                                   aaaaCGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCGACAGTAACCATCGCCGCACGAATCGTACCACCATTCAGCGTATAACCCATCTTCATAATGCCCAGTACGTTACCTgg               >  1:1492134/1‑148 (MQ=255)
                                                                                                                                        ggCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCaa          <  2:2840375/148‑1 (MQ=255)
                                                                                                                                            cGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc      <  1:1033655/148‑1 (MQ=255)
                                                                                                                                            cGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc      <  1:1033490/148‑1 (MQ=255)
                                                                                                                                             ggCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca     <  1:323540/148‑1 (MQ=255)
                                                                                                                                              gCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcat    >  2:2432547/1‑148 (MQ=255)
                                                                                                                                               cATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTCTAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc   >  2:1273573/1‑148 (MQ=255)
                                                                                                                                               cATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc   >  1:2812170/1‑148 (MQ=255)
                                                                                                                                               cATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc   >  2:1273667/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCA  >  CP009273/2743291‑2743582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATC  >  CP009273/2743293‑2743581
                                                                                                                                                 |                                                                                                                                               
CCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCAT                                                                                                                                             >  SRR19973065.590017/1‑150 (MQ=60)
 CCTGTCGCAGGCACCCGCGTGCTTATTGGCGAGTACTATATCTATATTCTCGACTTACAGCAAAATATTATTAAGAAGTTAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGTAGTAACGAAAACGGCCCGGCATTAGCATG                                                                                                                                            <  SRR19973065.2206092/150‑1 (MQ=60)
 CCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATG                                                                                                                                            >  SRR19973065.362823/1‑150 (MQ=60)
  CTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGATTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGC                                                                                                                                           >  SRR19973065.2858172/1‑150 (MQ=60)
  CTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGATTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGC                                                                                                                                           >  SRR19973065.2857863/1‑150 (MQ=60)
    GTCGCTGGCACCCGCGTGCTTATTGGCGATTACTATATCGATTTTCTCGCCGTACAGGAAAATATTATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAG                                                                                                                                         <  SRR19973065.2526453/150‑1 (MQ=60)
        CAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCC                                                                                                                                     >  SRR19973065.1655437/1‑150 (MQ=60)
         AGGCACCCGCGTGCGTATTGGCGAGTACTATATCGATATTCTCGACGTACAGTACAATTTGATTAAGAAGTTAAATGTTTTGCCGGTGAACCCGCTGGGCGAGAGGGGGGCGGAGTACCCAACACGGCCCGGCATTAGCATGCAGGGCCG                                                                                                                                    <  SRR19973065.353842/150‑1 (MQ=60)
         AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCG                                                                                                                                    >  SRR19973065.3337943/1‑150 (MQ=60)
              CCCGCGTGCGTTTGGGCGAGTACGATATCGATATTCTCGACGTACAGGAAAATTTTATTAAGCAGTTAAAATTTTTTCCTTTTAAACCGGTGCGCGTGTGTGTGGGGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAAT                                                                                                                               <  SRR19973065.330264/150‑1 (MQ=60)
              CCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATTATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAAT                                                                                                                               <  SRR19973065.2318939/150‑1 (MQ=60)
                  CGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATT                                                                                                                           >  SRR19973065.3287196/1‑150 (MQ=60)
                      CGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGA                                                                                                                       >  SRR19973065.803624/1‑150 (MQ=60)
                            ggcgataaacaaaaccaaatacaccACGTACAGCAAAATATTATTAAGCAGTTAAAATTTTTTCCTGTCAAACCGCTGCGCGAGAGTGTGGCGCATAAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAG                                                                                                                 <  SRR19973065.3259618/125‑1 (MQ=60)
                               GAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAA                                                                                                              >  SRR19973065.823118/1‑150 (MQ=60)
                                agtagtttatttatattctcgcggaccagcaaattattattaacaattaaaaatttctccttttcaatcggttgcgggtgtgggtgggggattaacaAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAT                                                                                                             <  SRR19973065.1815421/53‑1 (MQ=60)
                                 GTACTATATCGATATTCTCGACGTACTGGACAATATTATTAAGCAGGTAAATGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGGGGTGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATT                                                                                                            <  SRR19973065.3022873/150‑1 (MQ=60)
                                   ACTATATCGATTTTCTCGACGTACTGGACAATTTTTTTAAGCTTTTAAATGTTTTTCCTTTTAAACCGCTGGGCGATAGTGTGGGGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAA                                                                                                          <  SRR19973065.1874173/150‑1 (MQ=60)
                                   ACTATATCGATAGTCTCGACGTACTGTACAATATGATTAAGCTGTTAAAAGTTTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGGAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAA                                                                                                          <  SRR19973065.85850/150‑1 (MQ=60)
                                      ATATCTATATTCTCTACGTACAGGAAAATTTTATTAAGCATTTAAAAGTTTTTCCTGTTAAACCGCTGCGCGAGTGTGTGGCGTAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCT                                                                                                       <  SRR19973065.214931/150‑1 (MQ=60)
                                      ATATCGATATTCTCGACGTACAGGAAAATATTATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCT                                                                                                       <  SRR19973065.828988/150‑1 (MQ=60)
                                        atgtattggttcgtcgtataggacaatttttttatgctgttaatttttttgactgtgaacccggtgggcgagaggtTGGCGGTGTAACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTT                                                                                                     <  SRR19973065.788804/74‑1 (MQ=60)
                                        ATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTT                                                                                                     <  SRR19973065.2558620/150‑1 (MQ=60)
                                        ATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGTTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTT                                                                                                     <  SRR19973065.2637647/150‑1 (MQ=60)
                                          CGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTG                                                                                                   <  SRR19973065.1410535/150‑1 (MQ=60)
                                           GATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGTAATTAAGCTTTTGC                                                                                                  >  SRR19973065.3299481/1‑150 (MQ=60)
                                            ATATTCTCGACGTACAGGACAATATTATTAAGAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACTAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCT                                                                                                 <  SRR19973065.2478538/150‑1 (MQ=60)
                                             TATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTT                                                                                                >  SRR19973065.794369/1‑150 (MQ=60)
                                                  TCGACGTACAGTAAAATATGATTAAGCAGTTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGAAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCT                                                                                           <  SRR19973065.1585047/150‑1 (MQ=60)
                                                   CGACGTACAGGACAATATGATTAAGCAGGTAAATGTTGTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTA                                                                                          <  SRR19973065.3420524/150‑1 (MQ=60)
                                                   CGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTGTCCTGTGAACCCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTA                                                                                          <  SRR19973065.3419278/150‑1 (MQ=60)
                                                            GGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCA                                                                                 <  SRR19973065.3212836/150‑1 (MQ=60)
                                                               CAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCG                                                                              >  SRR19973065.3352944/1‑150 (MQ=60)
                                                                   ATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGC                                                                          >  SRR19973065.1787706/1‑150 (MQ=60)
                                                                      ATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTAAGAAAGCAGAAATTAATCTTTTGCTTTCGCTACAGTAACCATCGCCGCACG                                                                       >  SRR19973065.2417906/1‑150 (MQ=60)
                                                                          AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                   >  SRR19973065.2088860/1‑150 (MQ=60)
                                                                          AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATC                                                                   >  SRR19973065.140825/1‑150 (MQ=60)
                                                                          AGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAATAAGCATTTTCTTTCGCTACAGTAACCATCGCCGCACGAAAC                                                                   >  SRR19973065.163993/1‑150 (MQ=60)
                                                                            CTGGTAAAAGTTTGTCCTGTGAACCCGCTGCGCGAGAGGGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGT                                                                 <  SRR19973065.2475402/150‑1 (MQ=60)
                                                                            CAGGTAAATGTTTTGCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGT                                                                 <  SRR19973065.2475429/150‑1 (MQ=60)
                                                                            CAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGT                                                                 >  SRR19973065.2208015/1‑150 (MQ=60)
                                                                            CAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGT                                                                 >  SRR19973065.1877500/1‑150 (MQ=60)
                                                                            CAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGT                                                                 >  SRR19973065.427185/1‑150 (MQ=60)
                                                                             AGTTAAATGTTTTTCCTGTGAAACCGCTGCGCGTGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTA                                                                <  SRR19973065.1634068/150‑1 (MQ=60)
                                                                              GGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTAC                                                               >  SRR19973065.2016516/1‑150 (MQ=60)
                                                                                TAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGA                                                             >  SRR19973065.2514450/1‑150 (MQ=60)
                                                                                  AAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACC                                                           >  SRR19973065.1049095/1‑150 (MQ=60)
                                                                                  AAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACC                                                           >  SRR19973065.124949/1‑150 (MQ=60)
                                                                                  AAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACC                                                           >  SRR19973065.3513564/1‑150 (MQ=60)
                                                                                       TTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGGAGAAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCA                                                      <  SRR19973065.3081003/150‑1 (MQ=60)
                                                                                       TTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCA                                                      <  SRR19973065.3080484/150‑1 (MQ=60)
                                                                                           CCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGT                                                  >  SRR19973065.3042097/1‑150 (MQ=60)
                                                                                           CCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTAGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGT                                                  >  SRR19973065.1530082/1‑150 (MQ=60)
                                                                                                taacCCGCTGCGCGAGTGTGTGGCGGAGTAACCAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAC                                             <  SRR19973065.1232507/146‑1 (MQ=60)
                                                                                                GACACCGCTGCGCGAGAGTGTGGCGTAGTAACTAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAC                                             <  SRR19973065.1232568/150‑1 (MQ=60)
                                                                                                  AACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCC                                           >  SRR19973065.1349920/1‑150 (MQ=60)
                                                                                                    CCGCTGCGCTAGAGTGGGGCGGTGTATATAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTT                                         <  SRR19973065.2206796/150‑1 (MQ=60)
                                                                                                     CGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTC                                        >  SRR19973065.1138120/1‑150 (MQ=60)
                                                                                                     CGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTC                                        >  SRR19973065.1458652/1‑150 (MQ=60)
                                                                                                     CGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTC                                        >  SRR19973065.1137311/1‑150 (MQ=60)
                                                                                                       CTGCGCGTGTGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTG                                      <  SRR19973065.3117108/150‑1 (MQ=60)
                                                                                                       CTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTG                                      <  SRR19973065.241542/150‑1 (MQ=60)
                                                                                                          CGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAT                                   <  SRR19973065.1840022/150‑1 (MQ=60)
                                                                                                             GAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAAT                                >  SRR19973065.2487569/1‑150 (MQ=60)
                                                                                                                 GTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGTCC                            <  SRR19973065.891777/150‑1 (MQ=60)
                                                                                                                 GTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGTCC                            <  SRR19973065.891959/150‑1 (MQ=60)
                                                                                                                  TGTGGCGGTGTAACGAAAACGGCCCGGCATTAGCNTNCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTNCGNTACAGTAACCATCGCCGCACGAATNGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCA                           <  SRR19973065.3119120/150‑1 (MQ=60)
                                                                                                                       CGGATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACG                      <  SRR19973065.2493169/150‑1 (MQ=60)
                                                                                                                       CGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACG                      >  SRR19973065.1602286/1‑150 (MQ=60)
                                                                                                                       CGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAATAAGCTTTTGCTTTCGCTACATTAACCATAGCCGCACGAATCGTACGACCATTCAGCGTATAAACCTTCTGCATAATGCCCAGTAAG                      >  SRR19973065.3354569/1‑150 (MQ=60)
                                                                                                                          ATTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTA                   <  SRR19973065.2721852/150‑1 (MQ=60)
                                                                                                                          AGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTA                   <  SRR19973065.2721143/150‑1 (MQ=60)
                                                                                                                           GTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAC                  <  SRR19973065.956951/150‑1 (MQ=60)
                                                                                                                            TAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACC                 >  SRR19973065.3258747/1‑150 (MQ=60)
                                                                                                                             AACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCATCTGCATAATGCCCAGTACGTTACCT                >  SRR19973065.106677/1‑150 (MQ=60)
                                                                                                                              ccgacAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTG               <  SRR19973065.956133/145‑1 (MQ=60)
                                                                                                                              ACGAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGTCCAGTACGTTACCTG               <  SRR19973065.1352490/150‑1 (MQ=60)
                                                                                                                              ACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGTCCAGTACGTTACCTG               <  SRR19973065.1352499/150‑1 (MQ=60)
                                                                                                                               CGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGG              >  SRR19973065.1354973/1‑150 (MQ=60)
                                                                                                                               CGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCGACAGTAACCATCGCCGCACGAATCGTACCACCATTCAGCGTATAACCCATCTTCATAATGCCCAGTACGTTACCTGG              >  SRR19973065.1501708/1‑150 (MQ=60)
                                                                                                                                GAACACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGC             <  SRR19973065.805189/150‑1 (MQ=60)
                                                                                                                                          CGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCAT   <  SRR19973065.1040522/150‑1 (MQ=60)
                                                                                                                                          CGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCAT   <  SRR19973065.1040498/150‑1 (MQ=60)
                                                                                                                                          CGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCAT   <  SRR19973065.1040332/150‑1 (MQ=60)
                                                                                                                                           GGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATC  <  SRR19973065.325789/150‑1 (MQ=60)
                                                                                                                                           GGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATC  >  SRR19973065.2829557/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                               
CCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATC  >  CP009273/2743293‑2743581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑