Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,779,067 C→A 100% intergenic (‑697/+54) ypjC ← / ← ileY pseudogene/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,779,0670CA99.5% 668.5 / ‑4.6 205intergenic (‑697/+54)ypjC/ileYpseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (93/110);  total (93/112)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA  >  CP009273/2778920‑2779213
                                                                                                                                                   |                                                                                                                                                  
tAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGc                                                                                                                                                  <  2:4706073/150‑1 (MQ=255)
     ggCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCg                                                                                                                                             >  2:3288727/1‑150 (MQ=255)
     ggCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCg                                                                                                                                             <  2:5025718/150‑1 (MQ=255)
             ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcccc                                                                                                                                     <  2:1178750/150‑1 (MQ=255)
             ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcccc                                                                                                                                     >  1:2341730/1‑150 (MQ=255)
              tCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCt                                                                                                                                    <  2:2489897/150‑1 (MQ=255)
                 aGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGt                                                                                                                                 >  2:3502141/1‑150 (MQ=255)
                  ggTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTa                                                                                                                                >  1:2308819/1‑150 (MQ=255)
                   gTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTAc                                                                                                                               <  1:145472/150‑1 (MQ=255)
                     aCTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTAccc                                                                                                                             <  2:2539364/150‑1 (MQ=255)
                       ttttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCtt                                                                                                                           <  2:4972776/150‑1 (MQ=255)
                       ttttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCtt                                                                                                                           <  1:474441/150‑1 (MQ=255)
                         ttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTaa                                                                                                                         <  2:856286/150‑1 (MQ=255)
                          tGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAc                                                                                                                        >  1:1837509/1‑150 (MQ=255)
                           gAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                       <  2:4483147/150‑1 (MQ=255)
                              tGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCtt                                                                                                                    >  2:3636326/1‑150 (MQ=255)
                               gAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTc                                                                                                                   >  2:3828362/1‑150 (MQ=255)
                                  tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                <  1:2608988/150‑1 (MQ=255)
                                     tGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATa                                                                                                             >  2:3781204/1‑150 (MQ=255)
                                            tAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaat                                                                                                      >  1:2635442/1‑150 (MQ=255)
                                            tAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaat                                                                                                      >  2:1293945/1‑150 (MQ=255)
                                             aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                     >  2:1635474/1‑150 (MQ=255)
                                               aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataat                                                                                                   <  2:2175209/150‑1 (MQ=255)
                                               aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataat                                                                                                   <  2:289475/150‑1 (MQ=255)
                                                 aCATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGa                                                                                                 >  2:3194723/1‑150 (MQ=255)
                                                  cATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAt                                                                                                >  2:3135600/1‑150 (MQ=255)
                                                   aTTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAtt                                                                                               >  2:263529/1‑150 (MQ=255)
                                                     tGCAGTGGCGTGCCACGTACC‑AAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtgg                                                                                            >  1:57127/1‑150 (MQ=255)
                                                      gCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtgg                                                                                            >  1:3427208/1‑150 (MQ=255)
                                                       cAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggt                                                                                           >  2:1525992/1‑150 (MQ=255)
                                                       cAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggt                                                                                           <  1:2483655/150‑1 (MQ=255)
                                                        aGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggtg                                                                                          >  1:3340915/1‑150 (MQ=255)
                                                          tGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGc                                                                                        >  1:4924835/1‑150 (MQ=255)
                                                               tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                   >  1:3198845/1‑150 (MQ=255)
                                                               tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                   <  2:4625106/150‑1 (MQ=255)
                                                                gCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGc                                                                                  <  2:2373187/150‑1 (MQ=255)
                                                                 ccACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCt                                                                                 >  1:4330422/1‑150 (MQ=255)
                                                                 ccACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCt                                                                                 >  2:469196/1‑150 (MQ=255)
                                                                     gTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                             >  2:66278/1‑150 (MQ=255)
                                                                      tACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACt                                                                            >  2:3560801/1‑150 (MQ=255)
                                                                      tACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACt                                                                            <  1:3045708/150‑1 (MQ=255)
                                                                        ccAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTg                                                                          >  1:794445/1‑150 (MQ=255)
                                                                        ccAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATCGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTg                                                                          <  1:3711097/150‑1 (MQ=255)
                                                                         cAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGa                                                                         <  2:532114/150‑1 (MQ=255)
                                                                         cAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGa                                                                         <  2:2127209/150‑1 (MQ=255)
                                                                            aaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                      <  1:2056774/150‑1 (MQ=255)
                                                                            aaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                      >  1:626597/1‑150 (MQ=255)
                                                                                ccAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCg                                                                  <  2:4591738/150‑1 (MQ=255)
                                                                                 cAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGa                                                                 <  1:2895088/150‑1 (MQ=255)
                                                                                 cAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCATAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGa                                                                 >  1:5004751/1‑150 (MQ=255)
                                                                                   gCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGAcc                                                               >  2:3228317/1‑150 (MQ=255)
                                                                                   gCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGAcc                                                               <  2:2810036/150‑1 (MQ=255)
                                                                                     ccTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCaa                                                             <  2:3538509/150‑1 (MQ=255)
                                                                                     ccTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCACTTGTACCCTTAAATCTTCCTGATAAGTAAATACTGATTTGGTGGCGCTTGCTGGACTTGAACCAGCGACCaa                                                             <  1:2213645/150‑1 (MQ=255)
                                                                                        cATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCg                                                          <  1:1686176/150‑1 (MQ=255)
                                                                                         aTTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGa                                                         <  2:748485/150‑1 (MQ=255)
                                                                                         aTTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGa                                                         >  2:3010116/1‑150 (MQ=255)
                                                                                          ttCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAt                                                        <  2:4369339/150‑1 (MQ=255)
                                                                                             gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     >  2:1203442/1‑150 (MQ=255)
                                                                                             gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     <  2:391809/150‑1 (MQ=255)
                                                                                             gAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                     >  2:1903701/1‑150 (MQ=255)
                                                                                              aaaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATg                                                    >  1:4128327/1‑150 (MQ=255)
                                                                                              aaaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATg                                                    <  2:336413/150‑1 (MQ=255)
                                                                                                 ccacccacCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGt                                                 >  1:4704572/1‑150 (MQ=255)
                                                                                                  cacccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTc                                                <  1:1476331/150‑1 (MQ=255)
                                                                                                   acccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGTCACTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                               >  2:4794586/1‑150 (MQ=255)
                                                                                                     ccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGcc                                             <  2:1473958/150‑1 (MQ=255)
                                                                                                         cGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGct                                         >  1:2926003/1‑150 (MQ=255)
                                                                                                          gCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctc                                        >  1:1201308/1‑150 (MQ=255)
                                                                                                          gCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctc                                        >  1:2193631/1‑150 (MQ=255)
                                                                                                          gCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCATAACTATTCCTGATAAGTAAATAATGATTTGTTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctc                                        >  2:2439821/1‑150 (MQ=38)
                                                                                                           cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       >  1:4190491/1‑150 (MQ=255)
                                                                                                           cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                       <  1:1025505/150‑1 (MQ=255)
                                                                                                            aCTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTa                                      <  1:1525992/150‑1 (MQ=255)
                                                                                                            aCTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTa                                      <  2:370708/150‑1 (MQ=255)
                                                                                                                  tcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACt                                <  2:4173525/150‑1 (MQ=255)
                                                                                                                   ccTTGATATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               <  1:3420022/150‑1 (MQ=255)
                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               <  1:1668670/150‑1 (MQ=255)
                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               <  2:676328/150‑1 (MQ=255)
                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               >  2:2918866/1‑150 (MQ=255)
                                                                                                                   ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                               <  2:4250381/150‑1 (MQ=255)
                                                                                                                    cTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGa                              <  1:3102281/150‑1 (MQ=255)
                                                                                                                           tGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCCTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAg                       >  1:2132586/1‑150 (MQ=255)
                                                                                                                               gTTAGTCATGAAATATAGACATCCATCGAGTACCCCTTGTACCCTTAAGTCTTCCTGATAAGTAATTACTGATTTGGTGGCCCTTGCTGGAATTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGcc                   <  1:3547284/150‑1 (MQ=25)
                                                                                                                                ttAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCt                  <  2:781633/150‑1 (MQ=255)
                                                                                                                                 tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCtt                 <  1:4674848/150‑1 (MQ=255)
                                                                                                                                 tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCGTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCtt                 <  2:4383570/150‑1 (MQ=255)
                                                                                                                                   gTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGa               <  2:1037451/150‑1 (MQ=255)
                                                                                                                                     cATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCGTGAgt             >  2:1826795/1‑150 (MQ=255)
                                                                                                                                      aTGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgtg            >  1:3931049/1‑150 (MQ=255)
                                                                                                                                        gAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg          <  2:3414457/150‑1 (MQ=255)
                                                                                                                                          aaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCa        <  2:4054904/150‑1 (MQ=255)
                                                                                                                                          aaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCa        <  2:2562023/150‑1 (MQ=255)
                                                                                                                                           atctaGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCaa       <  2:4846819/147‑1 (MQ=255)
                                                                                                                                           atatAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCaa       >  2:310630/1‑150 (MQ=255)
                                                                                                                                           atatAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCaa       >  2:3098832/1‑150 (MQ=255)
                                                                                                                                           atatAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCaa       <  2:2286650/150‑1 (MQ=255)
                                                                                                                                               aGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAAc   <  1:4423827/150‑1 (MQ=255)
                                                                                                                                                gACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACa  <  2:1707055/150‑1 (MQ=255)
                                                                                                                                                gACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACa  <  2:4116922/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA  >  CP009273/2778920‑2779213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  CP009273/2778919‑2779216
                                                                                                                                                    |                                                                                                                                                     
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAG                                                                                                                                                      >  SRR19973063.4632391/1‑150 (MQ=60)
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAG                                                                                                                                                      >  SRR19973063.4883070/1‑150 (MQ=60)
    CAGGCAATTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                                  <  SRR19973063.3319809/150‑1 (MQ=60)
     AGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATC                                                                                                                                                 <  SRR19973063.2924395/150‑1 (MQ=60)
         ATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGT                                                                                                                                             <  SRR19973063.1049370/150‑1 (MQ=60)
          TTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTA                                                                                                                                            <  SRR19973063.2423588/150‑1 (MQ=60)
              TTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCC                                                                                                                                        >  SRR19973063.2349220/1‑150 (MQ=60)
                 CAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTG                                                                                                                                     >  SRR19973063.263748/1‑150 (MQ=60)
                   GGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTA                                                                                                                                   >  SRR19973063.2316198/1‑150 (MQ=60)
                   GGTACTTTTGAATAAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTA                                                                                                                                   <  SRR19973063.141875/150‑1 (MQ=60)
                    GTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTAC                                                                                                                                  <  SRR19973063.145996/150‑1 (MQ=60)
                    GTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTAC                                                                                                                                  <  SRR19973063.145922/150‑1 (MQ=60)
                      ACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCC                                                                                                                                <  SRR19973063.4748835/150‑1 (MQ=60)
                        TTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTT                                                                                                                              <  SRR19973063.475783/150‑1 (MQ=60)
                        TTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTT                                                                                                                              >  SRR19973063.756996/1‑150 (MQ=60)
                           TGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAC                                                                                                                           >  SRR19973063.1843243/1‑150 (MQ=60)
                                 AGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCC                                                                                                                     <  SRR19973063.3105783/150‑1 (MQ=60)
                                  GTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCT                                                                                                                    <  SRR19973063.5137985/150‑1 (MQ=60)
                                   TACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTG                                                                                                                   <  SRR19973063.2617277/150‑1 (MQ=60)
                                       GATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAC                                                                                                               <  SRR19973063.4051244/150‑1 (MQ=60)
                                        ATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACG                                                                                                              >  SRR19973063.920816/1‑150 (MQ=60)
                                             TAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAAT                                                                                                         >  SRR19973063.2235577/1‑150 (MQ=60)
                                             TAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAAT                                                                                                         >  SRR19973063.2643854/1‑150 (MQ=60)
                                                  ACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTGACTCTTCCTGATACGTAAATcagga                                                                                                    >  SRR19973063.1823352/1‑145 (MQ=60)
                                                      TGCAGTGGCGTGCCACGTACC‑AAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGG                                                                                               >  SRR19973063.57328/1‑150 (MQ=60)
                                                       GCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGG                                                                                               >  SRR19973063.3438106/1‑150 (MQ=60)
                                                        CAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGT                                                                                              <  SRR19973063.2491589/150‑1 (MQ=60)
                                                        CAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGT                                                                                              >  SRR19973063.3026518/1‑150 (MQ=60)
                                                        AAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTACTTGAAATTGCGTTAGTCATGAAATATAGACATCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGT                                                                                              <  SRR19973063.2491747/150‑1 (MQ=60)
                                                         AGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTG                                                                                             >  SRR19973063.3351566/1‑150 (MQ=60)
                                                         AGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTG                                                                                             >  SRR19973063.4312152/1‑150 (MQ=60)
                                                           TGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGC                                                                                           >  SRR19973063.4940738/1‑150 (MQ=60)
                                                                TGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTG                                                                                      >  SRR19973063.3209129/1‑150 (MQ=60)
                                                                 GCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTACATAATGAGTTGGTGGCCCTTGC                                                                                     >  SRR19973063.4399648/1‑150 (MQ=60)
                                                                  CCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCT                                                                                    >  SRR19973063.4344220/1‑150 (MQ=60)
                                                                   CACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTG                                                                                   <  SRR19973063.3702628/150‑1 (MQ=60)
                                                                    ACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGG                                                                                  <  SRR19973063.2103444/150‑1 (MQ=60)
                                                                       TACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACT                                                                               <  SRR19973063.3055451/150‑1 (MQ=60)
                                                                        ACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTT                                                                              >  SRR19973063.4886944/1‑150 (MQ=60)
                                                                         CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                             >  SRR19973063.796738/1‑150 (MQ=60)
                                                                         CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATCGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                             <  SRR19973063.3722961/150‑1 (MQ=60)
                                                                            AAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAC                                                                          <  SRR19973063.3741203/150‑1 (MQ=60)
                                                                             AACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACC                                                                         <  SRR19973063.2063200/150‑1 (MQ=60)
                                                                             AACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACC                                                                         >  SRR19973063.628435/1‑150 (MQ=60)
                                                                             AACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACC                                                                         >  SRR19973063.628440/1‑150 (MQ=60)
                                                                                  CAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGA                                                                    <  SRR19973063.2904323/150‑1 (MQ=60)
                                                                                  CAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCATAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGA                                                                    >  SRR19973063.5020911/1‑150 (MQ=60)
                                                                                    GCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACACTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAACAGCGACC                                                                  >  SRR19973063.4446699/1‑150 (MQ=60)
                                                                                      CCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAA                                                                <  SRR19973063.2220609/150‑1 (MQ=60)
                                                                                      CCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCACTTGTACCCTTAAATCTTCCTGATAAGTAAATACTGATTTGGTGGCGCTTGCTGGACTTGAACCAGCGACCAA                                                                <  SRR19973063.2220625/150‑1 (MQ=60)
                                                                                         CATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCG                                                             <  SRR19973063.1691312/150‑1 (MQ=60)
                                                                                          ATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGA                                                            >  SRR19973063.85943/1‑150 (MQ=60)
                                                                                               AAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATG                                                       >  SRR19973063.4141540/1‑150 (MQ=60)
                                                                                                 ACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAG                                                     >  SRR19973063.3084140/1‑150 (MQ=60)
                                                                                                  CCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGT                                                    >  SRR19973063.4719562/1‑150 (MQ=60)
                                                                                                   CACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTC                                                   <  SRR19973063.1480844/150‑1 (MQ=60)
                                                                                                     CCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGC                                                 <  SRR19973063.1543939/150‑1 (MQ=60)
                                                                                                          CGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCT                                            >  SRR19973063.2935331/1‑150 (MQ=60)
                                                                                                          CGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCT                                            >  SRR19973063.2347677/1‑150 (MQ=60)
                                                                                                           GCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTC                                           >  SRR19973063.2200522/1‑150 (MQ=60)
                                                                                                           GCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTC                                           >  SRR19973063.1204918/1‑150 (MQ=60)
                                                                                                            CACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCT                                          <  SRR19973063.1028597/150‑1 (MQ=60)
                                                                                                            CACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCT                                          >  SRR19973063.4203869/1‑150 (MQ=60)
                                                                                                            CACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCT                                          >  SRR19973063.4203858/1‑150 (MQ=60)
                                                                                                             ACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTA                                         <  SRR19973063.1530667/150‑1 (MQ=60)
                                                                                                             ACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTA                                         >  SRR19973063.2783148/1‑150 (MQ=60)
                                                                                                             ACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTA                                         >  SRR19973063.3464885/1‑150 (MQ=60)
                                                                                                                  TTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCAC                                    >  SRR19973063.1896383/1‑150 (MQ=60)
                                                                                                                    CCTTGATATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTG                                  <  SRR19973063.3430905/150‑1 (MQ=60)
                                                                                                                    CCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTG                                  <  SRR19973063.1673759/150‑1 (MQ=60)
                                                                                                                    CCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTG                                  <  SRR19973063.1673861/150‑1 (MQ=60)
                                                                                                                     CTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGA                                 <  SRR19973063.3112264/150‑1 (MQ=60)
                                                                                                                        GAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCT                              <  SRR19973063.3154959/150‑1 (MQ=60)
                                                                                                                            TGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCCTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAG                          >  SRR19973063.2139248/1‑150 (MQ=60)
                                                                                                                               CGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGC                       <  SRR19973063.4698229/150‑1 (MQ=60)
                                                                                                                               CGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGC                       >  SRR19973063.1514661/1‑150 (MQ=60)
                                                                                                                                GTTAGTCATGAAATATAGACATCCATCGAGTACCCCTTGTACCCTTAAGTCTTCCTGATAAGTAATTACTGATTTGGTGGCCCTTGCTGGAATTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCC                      <  SRR19973063.3558652/150‑1 (MQ=60)
                                                                                                                                GTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCTATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCC                      >  SRR19973063.3378919/1‑150 (MQ=60)
                                                                                                                                  TAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTT                    <  SRR19973063.4689759/150‑1 (MQ=60)
                                                                                                                                  TAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTT                    >  SRR19973063.3431877/1‑150 (MQ=60)
                                                                                                                                       ATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTG               >  SRR19973063.3943601/1‑150 (MQ=60)
                                                                                                                                               TAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA       <  SRR19973063.5018184/150‑1 (MQ=60)
                                                                                                                                                AGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAAC      <  SRR19973063.4438030/150‑1 (MQ=60)
                                                                                                                                                 GACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAAAA     <  SRR19973063.2171645/150‑1 (MQ=60)
                                                                                                                                                    AGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  SRR19973063.518730/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  CP009273/2778919‑2779216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑