Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,779,067 C→A 66.6% intergenic (‑697/+54) ypjC ← / ← ileY pseudogene/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,779,0670CA66.6% 212.9 / 242.0 201intergenic (‑697/+54)ypjC/ileYpseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base C (35/32);  new base A (63/71);  total (98/103)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 202 total aligned reads displayed.

TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  CP009273/2778920‑2779216
                                                                                                                                                   |                                                                                                                                                     
tAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGc                                                                                                                                                     >  1:870666/1‑150 (MQ=255)
 aGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGcc                                                                                                                                                    >  1:1584627/1‑150 (MQ=255)
        aTTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGt                                                                                                                                             >  2:4224307/1‑150 (MQ=255)
            tttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAccc                                                                                                                                         >  1:2786026/1‑150 (MQ=255)
             ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTAcccc                                                                                                                                        <  1:4745182/150‑1 (MQ=255)
             ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcccc                                                                                                                                        <  1:4846458/150‑1 (MQ=255)
             ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcccc                                                                                                                                        <  1:2252998/150‑1 (MQ=255)
             ttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAcccc                                                                                                                                        <  2:4702927/150‑1 (MQ=255)
               ccAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCtt                                                                                                                                      <  2:1281806/150‑1 (MQ=255)
               ccAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCtt                                                                                                                                      <  1:3241722/150‑1 (MQ=255)
               ccAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCtt                                                                                                                                      <  2:3518875/150‑1 (MQ=255)
                 aGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGt                                                                                                                                    >  1:1572426/1‑150 (MQ=255)
                    tACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTAcc                                                                                                                                 >  2:1875183/1‑150 (MQ=255)
                         ttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTaa                                                                                                                            <  2:1704741/150‑1 (MQ=255)
                         ttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTaa                                                                                                                            <  1:4413156/150‑1 (MQ=255)
                           gAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                          >  1:1758940/1‑150 (MQ=255)
                               gAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTc                                                                                                                      >  1:564048/1‑150 (MQ=255)
                               gAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTc                                                                                                                      <  2:2064939/150‑1 (MQ=255)
                                 gTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCt                                                                                                                    >  1:229208/1‑150 (MQ=255)
                                  tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                   <  1:51621/150‑1 (MQ=255)
                                  tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                                   <  1:53515/150‑1 (MQ=255)
                                     tGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTCCCCCTGGTACCCTTCACTCTTCCTGATa                                                                                                                >  2:2628266/1‑150 (MQ=255)
                                      gATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAc                                                                                                               <  1:983858/150‑1 (MQ=255)
                                         ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTa                                                                                                            >  1:3029526/1‑150 (MQ=255)
                                          gATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTaa                                                                                                           >  2:375983/1‑150 (MQ=255)
                                           aTAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTaaa                                                                                                          >  1:1125223/1‑150 (MQ=255)
                                           aTAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTaaa                                                                                                          >  2:2743011/1‑150 (MQ=255)
                                           aTAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTaaa                                                                                                          >  2:2979515/1‑150 (MQ=255)
                                             aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                        <  1:65728/150‑1 (MQ=255)
                                             aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                        <  1:2437251/150‑1 (MQ=255)
                                             aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                        <  2:2527174/150‑1 (MQ=255)
                                                tACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATg                                                                                                     >  1:3087987/1‑150 (MQ=255)
                                                  cATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAt                                                                                                   >  2:839753/1‑150 (MQ=255)
                                                  cATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAt                                                                                                   <  2:4421248/150‑1 (MQ=255)
                                                    ttGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAttt                                                                                                 <  2:3069437/150‑1 (MQ=255)
                                                     tGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtg                                                                                                <  2:1516628/150‑1 (MQ=255)
                                                     tGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtg                                                                                                <  1:1729703/150‑1 (MQ=255)
                                                      gCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtgg                                                                                               >  2:2598710/1‑150 (MQ=255)
                                                      gCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtgg                                                                                               <  1:2016477/150‑1 (MQ=255)
                                                       cAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggt                                                                                              <  2:4804421/150‑1 (MQ=255)
                                                         gTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggtgg                                                                                            >  2:485690/1‑150 (MQ=255)
                                                          tGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGAGACGTAAATAATGATTTGGTGGc                                                                                           >  2:2133647/1‑150 (MQ=255)
                                                              gTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCtt                                                                                       <  2:3179300/150‑1 (MQ=255)
                                                               tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                      >  2:1078335/1‑150 (MQ=255)
                                                               tGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTg                                                                                      >  2:1872084/1‑150 (MQ=255)
                                                                 ccACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCt                                                                                    <  2:4784707/150‑1 (MQ=255)
                                                                  cACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTg                                                                                   >  2:3979589/1‑150 (MQ=255)
                                                                  cACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTg                                                                                   <  2:950373/150‑1 (MQ=255)
                                                                    cGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGa                                                                                 >  1:2909823/1‑150 (MQ=255)
                                                                    cGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGa                                                                                 >  1:479669/1‑150 (MQ=255)
                                                                     gTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                                >  2:1722905/1‑150 (MQ=255)
                                                                     gTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                                >  2:3324521/1‑150 (MQ=255)
                                                                         cAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGa                                                                            <  2:933276/150‑1 (MQ=255)
                                                                            aaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                         <  1:668411/150‑1 (MQ=255)
                                                                            aaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                         >  1:415089/1‑150 (MQ=255)
                                                                              cacCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAg                                                                       <  2:3167676/150‑1 (MQ=255)
                                                                               acCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGc                                                                      >  2:3582944/1‑150 (MQ=255)
                                                                                     ccTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCaa                                                                <  1:22414/150‑1 (MQ=255)
                                                                                     ccTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATACTGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCaa                                                                <  2:4160196/150‑1 (MQ=255)
                                                                                         aTTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGa                                                            >  2:1183344/1‑150 (MQ=255)
                                                                                          ttCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAt                                                           >  1:2062265/1‑150 (MQ=255)
                                                                                           tCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAtt                                                          >  1:3187389/1‑150 (MQ=255)
                                                                                           tCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAATCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAtt                                                          >  1:2076495/1‑150 (MQ=255)
                                                                                            cGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTa                                                         <  2:4804746/150‑1 (MQ=255)
                                                                                              aaaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTacg                                                       >  2:1444066/1‑148 (MQ=255)
                                                                                               aaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGa                                                      <  1:3438599/150‑1 (MQ=255)
                                                                                               aaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGa                                                      >  2:707150/1‑150 (MQ=255)
                                                                                                   acccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCg                                                  <  1:979176/150‑1 (MQ=255)
                                                                                                       accGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTg                                              >  1:2962116/1‑150 (MQ=255)
                                                                                                         cGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGct                                            <  1:1501613/150‑1 (MQ=255)
                                                                                                           cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                          <  1:3233901/150‑1 (MQ=255)
                                                                                                           cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                          >  2:2537010/1‑150 (MQ=255)
                                                                                                               tcttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAcc                                      >  2:4565672/1‑150 (MQ=255)
                                                                                                               tcttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAAcc                                      >  2:4565313/1‑150 (MQ=255)
                                                                                                                cttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCa                                     >  2:468240/1‑150 (MQ=255)
                                                                                                                cttcCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAGTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCa                                     >  2:4684828/1‑150 (MQ=255)
                                                                                                                cttcCTTGAAATGGCGTTAGTCAAGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCa                                     <  2:3392406/150‑1 (MQ=255)
                                                                                                                     ttGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAg                                >  2:2557682/1‑150 (MQ=255)
                                                                                                                        aaaTGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTa                             <  2:3199308/150‑1 (MQ=255)
                                                                                                                            ggCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAgg                         <  2:2903343/150‑1 (MQ=255)
                                                                                                                               gTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGcc                      >  1:1071319/1‑150 (MQ=255)
                                                                                                                                 tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCtt                    <  1:4559237/150‑1 (MQ=255)
                                                                                                                                  aGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTg                   <  1:3976479/150‑1 (MQ=255)
                                                                                                                                  aGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTg                   <  2:870666/150‑1 (MQ=255)
                                                                                                                                   gTCATGAAATATGGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGa                  >  1:3129292/1‑150 (MQ=255)
                                                                                                                                    tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAg                 >  1:3420953/1‑150 (MQ=255)
                                                                                                                                    tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACACTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCGAAAGGGCCTTGAg                 >  1:3961053/1‑150 (MQ=255)
                                                                                                                                     cATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgt                <  2:564048/150‑1 (MQ=255)
                                                                                                                                       tGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgtgt              <  1:4800169/150‑1 (MQ=255)
                                                                                                                                       tGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgtgt              <  2:3081738/150‑1 (MQ=255)
                                                                                                                                        gAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg             <  2:1393802/150‑1 (MQ=255)
                                                                                                                                        gAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg             <  1:762078/150‑1 (MQ=255)
                                                                                                                                        gAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGtgtg             <  2:2927028/150‑1 (MQ=255)
                                                                                                                                          aaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCa           <  2:627120/150‑1 (MQ=255)
                                                                                                                                            tataGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAt         >  2:1983625/1‑150 (MQ=255)
                                                                                                                                             ataGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa        <  2:201401/150‑1 (MQ=255)
                                                                                                                                             ataGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATa        <  2:1584615/150‑1 (MQ=255)
                                                                                                                                              taGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATaa       >  1:1293867/1‑150 (MQ=255)
                                                                                                                                              taGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATaa       <  1:4568204/150‑1 (MQ=255)
                                                                                                                                              taGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATaa       <  1:3538264/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  CP009273/2778920‑2779216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  CP009273/2778919‑2779216
                                                                                                                                                    |                                                                                                                                                     
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAG                                                                                                                                                      <  SRR19973081.721118/150‑1 (MQ=60)
 TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGC                                                                                                                                                     >  SRR19973081.876404/1‑150 (MQ=60)
 TAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGC                                                                                                                                                     >  SRR19973081.2224428/1‑150 (MQ=60)
 TAGCAGGCATTTTTTCCAGGTACTTTAGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTACTTCCTTGAAATGGCGTTAGTCATGAAATATATACAGC                                                                                                                                                     >  SRR19973081.280933/1‑150 (MQ=60)
  AGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCC                                                                                                                                                    >  SRR19973081.1594810/1‑150 (MQ=60)
  AGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCC                                                                                                                                                    >  SRR19973081.1594822/1‑150 (MQ=60)
      GGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATGGACCGCCATCG                                                                                                                                                <  SRR19973081.670981/150‑1 (MQ=60)
      GGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCG                                                                                                                                                >  SRR19973081.1403147/1‑150 (MQ=60)
         ATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGT                                                                                                                                             <  SRR19973081.3467646/150‑1 (MQ=60)
             TTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCC                                                                                                                                         >  SRR19973081.2803056/1‑150 (MQ=60)
              TTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCC                                                                                                                                        <  SRR19973081.4773883/150‑1 (MQ=60)
              TTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCC                                                                                                                                        <  SRR19973081.4875560/150‑1 (MQ=60)
              TTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCC                                                                                                                                        <  SRR19973081.4733636/150‑1 (MQ=60)
              TTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCC                                                                                                                                        <  SRR19973081.2267239/150‑1 (MQ=60)
                CCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTT                                                                                                                                      <  SRR19973081.1094207/150‑1 (MQ=60)
                CCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTT                                                                                                                                      <  SRR19973081.3261233/150‑1 (MQ=60)
                  AGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGT                                                                                                                                    >  SRR19973081.1582582/1‑150 (MQ=60)
                  AGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTGCTTCCTTGAAATGGCGTTAGTCTTGAAATATAGACAGCCATCGAGTACCCCTTGT                                                                                                                                    >  SRR19973081.1581900/1‑150 (MQ=60)
                        TTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTT                                                                                                                              >  SRR19973081.631487/1‑150 (MQ=60)
                          TTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAA                                                                                                                            <  SRR19973081.4439728/150‑1 (MQ=60)
                            GAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACT                                                                                                                          >  SRR19973081.1770458/1‑150 (MQ=60)
                                GAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTC                                                                                                                      >  SRR19973081.568058/1‑150 (MQ=60)
                                 AGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCC                                                                                                                     <  SRR19973081.3255262/150‑1 (MQ=60)
                                 AGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCC                                                                                                                     <  SRR19973081.3128927/150‑1 (MQ=60)
                                  GTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCT                                                                                                                    >  SRR19973081.231119/1‑150 (MQ=60)
                                   TACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTG                                                                                                                   <  SRR19973081.831566/150‑1 (MQ=60)
                                   TACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTG                                                                                                                   <  SRR19973081.52224/150‑1 (MQ=60)
                                   TACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTG                                                                                                                   <  SRR19973081.54150/150‑1 (MQ=60)
                                       GATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAC                                                                                                               <  SRR19973081.990553/150‑1 (MQ=60)
                                          GGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTA                                                                                                            >  SRR19973081.3100666/1‑150 (MQ=60)
                                          GGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTA                                                                                                            <  SRR19973081.2283558/150‑1 (MQ=60)
                                          GGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTA                                                                                                            >  SRR19973081.3048190/1‑150 (MQ=60)
                                           GATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAA                                                                                                           >  SRR19973081.2663687/1‑150 (MQ=60)
                                            ATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAA                                                                                                          >  SRR19973081.1132578/1‑150 (MQ=60)
                                              AAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATA                                                                                                        <  SRR19973081.66490/150‑1 (MQ=60)
                                              AAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATA                                                                                                        <  SRR19973081.2452241/150‑1 (MQ=60)
                                               AATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAA                                                                                                       >  SRR19973081.956797/1‑150 (MQ=60)
                                                 TACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATG                                                                                                     >  SRR19973081.3106947/1‑150 (MQ=60)
                                                 TACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATACAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATG                                                                                                     >  SRR19973081.4173923/1‑150 (MQ=60)
                                                    ATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATT                                                                                                  >  SRR19973081.3822626/1‑150 (MQ=60)
                                                      TGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTG                                                                                                <  SRR19973081.1741051/150‑1 (MQ=60)
                                                       GCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGG                                                                                               <  SRR19973081.2029045/150‑1 (MQ=60)
                                                       GCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGG                                                                                               <  SRR19973081.501738/150‑1 (MQ=60)
                                                         AGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTG                                                                                             >  SRR19973081.3384145/1‑150 (MQ=60)
                                                                   CACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTG                                                                                   >  SRR19973081.4809405/1‑150 (MQ=60)
                                                                     CGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGA                                                                                 >  SRR19973081.2927753/1‑150 (MQ=60)
                                                                     CGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGA                                                                                 >  SRR19973081.483043/1‑150 (MQ=60)
                                                                       TACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACT                                                                               <  SRR19973081.562128/150‑1 (MQ=60)
                                                                             AACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACC                                                                         <  SRR19973081.672957/150‑1 (MQ=60)
                                                                             AACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACC                                                                         <  SRR19973081.673909/150‑1 (MQ=60)
                                                                             AACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACC                                                                         >  SRR19973081.417784/1‑150 (MQ=60)
                                                                              ACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCA                                                                        <  SRR19973081.2258454/150‑1 (MQ=60)
                                                                                  CAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGA                                                                    >  SRR19973081.3227027/1‑150 (MQ=60)
                                                                                      CCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAA                                                                >  SRR19973081.3032439/1‑150 (MQ=60)
                                                                                      CCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAA                                                                <  SRR19973081.22696/150‑1 (MQ=60)
                                                                                           TTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAT                                                           >  SRR19973081.2075283/1‑150 (MQ=60)
                                                                                           TTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAT                                                           >  SRR19973081.2075298/1‑150 (MQ=60)
                                                                                           TTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAT                                                           >  SRR19973081.2076822/1‑150 (MQ=60)
                                                                                            TCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATT                                                          >  SRR19973081.3206152/1‑150 (MQ=60)
                                                                                            TCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATT                                                          >  SRR19973081.3206727/1‑150 (MQ=60)
                                                                                            TCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAATCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATT                                                          >  SRR19973081.2089659/1‑150 (MQ=60)
                                                                                            TCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAATCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATT                                                          >  SRR19973081.4557245/1‑150 (MQ=60)
                                                                                               AAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATG                                                       <  SRR19973081.1984688/150‑1 (MQ=60)
                                                                                                AACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGA                                                      <  SRR19973081.3459749/150‑1 (MQ=60)
                                                                                                    ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                                  <  SRR19973081.985921/150‑1 (MQ=60)
                                                                                                    ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                                  <  SRR19973081.985841/150‑1 (MQ=60)
                                                                                                      CCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTGCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCC                                                >  SRR19973081.243367/1‑150 (MQ=60)
                                                                                                        ACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTG                                              >  SRR19973081.2980379/1‑150 (MQ=60)
                                                                                                          CGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCT                                            <  SRR19973081.1511525/150‑1 (MQ=60)
                                                                                                            CACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCT                                          <  SRR19973081.3253385/150‑1 (MQ=60)
                                                                                                                               CGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGC                       <  SRR19973081.3447013/150‑1 (MQ=60)
                                                                                                                                GTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCC                      >  SRR19973081.1078445/1‑150 (MQ=60)
                                                                                                                                  TAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTT                    <  SRR19973081.4586889/150‑1 (MQ=60)
                                                                                                                                   AGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTG                   <  SRR19973081.4000730/150‑1 (MQ=60)
                                                                                                                                   AGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTG                   <  SRR19973081.4000729/150‑1 (MQ=60)
                                                                                                                                    GTCATGAAATATGGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGA                  >  SRR19973081.3148439/1‑150 (MQ=60)
                                                                                                                                    GTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAGTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGA                  >  SRR19973081.1179433/1‑150 (MQ=60)
                                                                                                                                     TCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAG                 >  SRR19973081.3441989/1‑150 (MQ=60)
                                                                                                                                     TCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAG                 >  SRR19973081.2930057/1‑150 (MQ=60)
                                                                                                                                     TCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACACTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCGAAAGGGCCTTGAG                 >  SRR19973081.3985273/1‑150 (MQ=60)
                                                                                                                                        TGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGT              <  SRR19973081.4829094/150‑1 (MQ=60)
                                                                                                                                        TGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGT              >  SRR19973081.4860545/1‑150 (MQ=60)
                                                                                                                                        TGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGT              <  SRR19973081.4829070/150‑1 (MQ=60)
                                                                                                                                         GAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTG             <  SRR19973081.767004/150‑1 (MQ=60)
                                                                                                                                         GAAATATAGACAGCCATCGAGTACCCCGTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTG             <  SRR19973081.524451/150‑1 (MQ=60)
                                                                                                                                               TAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA       >  SRR19973081.1302469/1‑150 (MQ=60)
                                                                                                                                               TAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA       <  SRR19973081.4595905/150‑1 (MQ=60)
                                                                                                                                               TAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA       <  SRR19973081.1742299/150‑1 (MQ=60)
                                                                                                                                               TAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA       <  SRR19973081.4595904/150‑1 (MQ=60)
                                                                                                                                               TAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA       <  SRR19973081.3559875/150‑1 (MQ=60)
                                                                                                                                                 GACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACA     >  SRR19973081.4241682/1‑150 (MQ=60)
                                                                                                                                                   CAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAAT   >  SRR19973081.2915255/1‑150 (MQ=60)
                                                                                                                                                    CGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  <  SRR19973081.1703819/150‑1 (MQ=60)
                                                                                                                                                    AGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  <  SRR19973081.79443/150‑1 (MQ=60)
                                                                                                                                                    AGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  <  SRR19973081.4148709/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAACAATA  >  CP009273/2778919‑2779216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑