Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,779,067 C→A 100% intergenic (‑697/+54) ypjC ← / ← ileY pseudogene/tRNA‑Ile

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,779,0670CA100.0% 796.6 / NA 239intergenic (‑697/+54)ypjC/ileYpseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (125/114);  total (125/114)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA  >  CP009273/2778923‑2779211
                                                                                                                                                |                                                                                                                                                
cAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGcc                                                                                                                                               <  1:490223/148‑1 (MQ=255)
cAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGcc                                                                                                                                               >  2:3320568/1‑148 (MQ=255)
  ggCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAt                                                                                                                                             >  1:328073/1‑148 (MQ=255)
  ggCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAt                                                                                                                                             <  2:1560640/148‑1 (MQ=255)
  ggCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAt                                                                                                                                             >  1:1111852/1‑148 (MQ=255)
  ggCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAt                                                                                                                                             <  1:3532170/148‑1 (MQ=255)
   gCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATc                                                                                                                                            >  1:822686/1‑148 (MQ=255)
   gCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATc                                                                                                                                            >  1:2737220/1‑148 (MQ=255)
      ttttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAg                                                                                                                                         >  1:1796480/1‑148 (MQ=255)
      ttttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAg                                                                                                                                         >  2:3459084/1‑148 (MQ=255)
       tttttCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGt                                                                                                                                        <  1:953184/148‑1 (MQ=255)
           tCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAccc                                                                                                                                    <  1:3094267/148‑1 (MQ=255)
              aGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCtt                                                                                                                                 >  1:1398859/1‑148 (MQ=255)
               ggTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTg                                                                                                                                >  1:1987685/1‑148 (MQ=255)
               ggTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTg                                                                                                                                >  2:3245058/1‑148 (MQ=255)
                gTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGt                                                                                                                               >  2:3808075/1‑148 (MQ=255)
                gTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGt                                                                                                                               >  2:113630/1‑148 (MQ=255)
                gTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGt                                                                                                                               >  1:693511/1‑148 (MQ=255)
                gTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGt                                                                                                                               >  1:1478746/1‑148 (MQ=255)
                 tACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTa                                                                                                                              <  1:857702/148‑1 (MQ=255)
                 tACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTa                                                                                                                              <  2:530318/148‑1 (MQ=255)
                    ttttGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTAccc                                                                                                                           >  1:2643246/1‑148 (MQ=255)
                       tGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTa                                                                                                                        <  2:1746274/148‑1 (MQ=255)
                        gAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTaa                                                                                                                       <  2:1340725/148‑1 (MQ=255)
                          aTGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                     <  1:1017032/148‑1 (MQ=255)
                          aTGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAAct                                                                                                                     <  1:2922841/148‑1 (MQ=255)
                           tGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAActc                                                                                                                    >  1:2890347/1‑148 (MQ=255)
                             aGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACAGCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCtt                                                                                                                  >  2:2255425/1‑148 (MQ=255)
                              gTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCGTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTc                                                                                                                 <  2:1512728/148‑1 (MQ=255)
                               tACTGATGGATAAATACATTGCAGTGGTGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTcc                                                                                                                <  1:581651/148‑1 (MQ=255)
                               tACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTcc                                                                                                                <  1:1950326/148‑1 (MQ=255)
                                aCTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCt                                                                                                               >  1:3408175/1‑148 (MQ=255)
                                 cTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTg                                                                                                              <  2:3327187/148‑1 (MQ=255)
                                  tGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGa                                                                                                             >  2:61404/1‑148 (MQ=255)
                                   gATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGAt                                                                                                            <  1:3602884/148‑1 (MQ=255)
                                     tGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAc                                                                                                          >  2:532137/1‑148 (MQ=255)
                                      ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACg                                                                                                         <  2:2279517/148‑1 (MQ=255)
                                      ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACg                                                                                                         <  1:235955/148‑1 (MQ=255)
                                      ggATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACg                                                                                                         >  1:479714/1‑148 (MQ=255)
                                       gATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGt                                                                                                        >  2:827892/1‑148 (MQ=255)
                                          aaaTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTaaa                                                                                                     >  2:2302449/1‑148 (MQ=255)
                                            aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                   <  1:1035486/148‑1 (MQ=255)
                                            aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                   >  2:1908502/1‑148 (MQ=255)
                                            aTACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaata                                                                                                   <  2:3569389/148‑1 (MQ=255)
                                             tACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataa                                                                                                  >  1:240710/1‑148 (MQ=255)
                                             tACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataa                                                                                                  <  2:1307104/148‑1 (MQ=255)
                                              aCATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataat                                                                                                 >  1:2052867/1‑148 (MQ=255)
                                              aCATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAaataat                                                                                                 >  1:859833/1‑148 (MQ=255)
                                                aTTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGa                                                                                               >  1:2176414/1‑148 (MQ=255)
                                                aTTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGa                                                                                               <  1:2262976/148‑1 (MQ=255)
                                                 ttGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAt                                                                                              <  2:904898/148‑1 (MQ=255)
                                                 ttGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAt                                                                                              >  1:3468322/1‑148 (MQ=255)
                                                      gTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTtggt                                                                                         <  2:3206531/148‑1 (MQ=255)
                                                           gTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGccc                                                                                    <  2:1627592/148‑1 (MQ=255)
                                                                   tACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGa                                                                            <  2:2737220/148‑1 (MQ=255)
                                                                   tACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGa                                                                            <  2:383484/148‑1 (MQ=255)
                                                                    aCCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                           <  2:3523990/148‑1 (MQ=255)
                                                                    aCCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAc                                                                           >  2:2084452/1‑148 (MQ=255)
                                                                     ccAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACt                                                                          <  1:106488/148‑1 (MQ=255)
                                                                     ccAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACt                                                                          >  1:2140036/1‑148 (MQ=255)
                                                                      cAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGACCTTGCTGGACtt                                                                         >  2:1088612/1‑148 (MQ=255)
                                                                       aaaaCACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTg                                                                        >  1:3140819/1‑148 (MQ=255)
                                                                          acacCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAc                                                                     <  2:1536679/148‑1 (MQ=255)
                                                                           cacCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAAcc                                                                    <  2:3084340/148‑1 (MQ=255)
                                                                              cAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGc                                                                 <  2:631875/148‑1 (MQ=255)
                                                                                gCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGa                                                               >  2:2707095/1‑148 (MQ=255)
                                                                                   cTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCa                                                            >  2:3488745/1‑148 (MQ=255)
                                                                                    tCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCaa                                                           >  1:2798718/1‑148 (MQ=255)
                                                                                     cATTCGAAACCACCCACCGCACGTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAg                                                          >  2:252124/1‑148 (MQ=255)
                                                                                        tCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCGTGCTGGACTTGAACCAGCGACCAAGCGa                                                       <  2:3732322/148‑1 (MQ=255)
                                                                                         cGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGAt                                                      <  2:282917/148‑1 (MQ=255)
                                                                                           aaaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTa                                                    >  2:3655018/1‑148 (MQ=255)
                                                                                            aaCCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATATACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAt                                                   >  1:2677774/1‑148 (MQ=255)
                                                                                             accaccCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATg                                                  >  1:1269062/1‑148 (MQ=255)
                                                                                               cacccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAg                                                >  1:3321801/1‑148 (MQ=255)
                                                                                                acccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGt                                               <  1:2483876/148‑1 (MQ=255)
                                                                                                 cccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTc                                              >  1:319722/1‑148 (MQ=255)
                                                                                                 cccaccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTc                                              >  2:2869863/1‑148 (MQ=255)
                                                                                                   caccGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGc                                            >  1:3711178/1‑148 (MQ=255)
                                                                                                        cACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGct                                       <  1:3067389/148‑1 (MQ=255)
                                                                                                          cttcttCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGctct                                     >  2:193868/1‑148 (MQ=255)
                                                                                                                ccTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCAc                               <  1:2261352/148‑1 (MQ=255)
                                                                                                                  ttGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                             <  2:51497/148‑1 (MQ=255)
                                                                                                                  ttGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTg                             <  2:1394926/148‑1 (MQ=255)
                                                                                                                   tGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGa                            >  2:2407900/1‑148 (MQ=255)
                                                                                                                     aaaTGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGc                          >  2:1244745/1‑148 (MQ=255)
                                                                                                                        tGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTaa                       >  1:596779/1‑148 (MQ=255)
                                                                                                                          gCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAg                     <  1:3476784/148‑1 (MQ=255)
                                                                                                                              tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGcc                 <  1:727358/148‑1 (MQ=255)
                                                                                                                              tAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATCATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGcc                 >  1:1672669/1‑148 (MQ=255)
                                                                                                                                 tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTg              <  1:166265/148‑1 (MQ=255)
                                                                                                                                 tCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTg              >  2:1162833/1‑148 (MQ=255)
                                                                                                                                    tGAAATATAGACAGCCATCTAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgt           >  2:2470724/1‑148 (MQ=255)
                                                                                                                                    tGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgt           <  2:2191131/148‑1 (MQ=255)
                                                                                                                                      aaaTATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAgtgt         >  1:622692/1‑148 (MQ=255)
                                                                                                                                        atatAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGc       >  1:1819919/1‑148 (MQ=255)
                                                                                                                                        atatAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGc       >  1:3230329/1‑148 (MQ=255)
                                                                                                                                         tataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCa      <  1:113630/148‑1 (MQ=255)
                                                                                                                                         tataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCAATGAGCTAAAGGGCCTTGAGTGTGCa      <  1:1088612/148‑1 (MQ=255)
                                                                                                                                          ataGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCaa     >  2:2792088/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAATAA  >  CP009273/2778923‑2779211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAT  >  CP009273/2778918‑2779209
                                                                                                                                                     |                                                                                                                                              
CATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACA                                                                                                                                                <  SRR19973069.2776067/150‑1 (MQ=60)
     CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                           >  SRR19973069.330289/1‑150 (MQ=60)
     CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                           >  SRR19973069.2224732/1‑150 (MQ=60)
     CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                           <  SRR19973069.493756/150‑1 (MQ=60)
     CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                           <  SRR19973069.493745/150‑1 (MQ=60)
     CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                           <  SRR19973069.2744116/150‑1 (MQ=60)
     CAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCAT                                                                                                                                           >  SRR19973069.1119610/1‑150 (MQ=60)
      AGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATC                                                                                                                                          >  SRR19973069.2755530/1‑150 (MQ=60)
      AGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATC                                                                                                                                          >  SRR19973069.386291/1‑150 (MQ=60)
      AGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATC                                                                                                                                          >  SRR19973069.828490/1‑150 (MQ=60)
       GGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCG                                                                                                                                         <  SRR19973069.3158072/150‑1 (MQ=60)
       GGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCG                                                                                                                                         <  SRR19973069.3555052/150‑1 (MQ=60)
         CATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAG                                                                                                                                       >  SRR19973069.1809132/1‑150 (MQ=60)
          ATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGT                                                                                                                                      >  SRR19973069.1304859/1‑150 (MQ=60)
           TTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTA                                                                                                                                     <  SRR19973069.2772176/150‑1 (MQ=60)
            TTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAC                                                                                                                                    <  SRR19973069.959227/150‑1 (MQ=60)
            TTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTAC                                                                                                                                    <  SRR19973069.959583/150‑1 (MQ=60)
                TCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCT                                                                                                                                <  SRR19973069.3114659/150‑1 (MQ=60)
                TCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCT                                                                                                                                <  SRR19973069.620784/150‑1 (MQ=60)
                 CCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCNTTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTT                                                                                                                               <  SRR19973069.1950717/150‑1 (MQ=60)
                 CCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTT                                                                                                                               <  SRR19973069.809152/150‑1 (MQ=60)
                 CCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTT                                                                                                                               >  SRR19973069.1408623/1‑150 (MQ=60)
                 CCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTT                                                                                                                               <  SRR19973069.1871044/150‑1 (MQ=60)
                  CAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTG                                                                                                                              >  SRR19973069.2001532/1‑150 (MQ=60)
                   AGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGT                                                                                                                             >  SRR19973069.1489035/1‑150 (MQ=60)
                   AGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGT                                                                                                                             >  SRR19973069.698378/1‑150 (MQ=60)
                   AGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGT                                                                                                                             >  SRR19973069.3494142/1‑150 (MQ=60)
                      TACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACC                                                                                                                          <  SRR19973069.863706/150‑1 (MQ=60)
                      TACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACC                                                                                                                          <  SRR19973069.2647237/150‑1 (MQ=60)
                       ACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCC                                                                                                                         >  SRR19973069.2660949/1‑150 (MQ=60)
                        CTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCT                                                                                                                        >  SRR19973069.2466628/1‑150 (MQ=60)
                              AATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTC                                                                                                                  >  SRR19973069.2909396/1‑150 (MQ=60)
                               ATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCT                                                                                                                 <  SRR19973069.2942199/150‑1 (MQ=60)
                               ATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCT                                                                                                                 <  SRR19973069.2942198/150‑1 (MQ=60)
                               ATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCT                                                                                                                 <  SRR19973069.1024052/150‑1 (MQ=60)
                                TGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTT                                                                                                                <  SRR19973069.689133/150‑1 (MQ=60)
                                   GTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCT                                                                                                             <  SRR19973069.1139117/150‑1 (MQ=60)
                                   GTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCT                                                                                                             >  SRR19973069.3430391/1‑150 (MQ=60)
                                   GTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCT                                                                                                             >  SRR19973069.2173949/1‑150 (MQ=60)
                                   GTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCT                                                                                                             <  SRR19973069.865697/150‑1 (MQ=60)
                                    TACTGATGGATAAATACATTGCAGTGGTGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTG                                                                                                            <  SRR19973069.585638/150‑1 (MQ=60)
                                    TACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTG                                                                                                            <  SRR19973069.1963806/150‑1 (MQ=60)
                                      CTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGAT                                                                                                          <  SRR19973069.1425276/150‑1 (MQ=60)
                                        GATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATAC                                                                                                        <  SRR19973069.3626403/150‑1 (MQ=60)
                                         ATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACG                                                                                                       >  SRR19973069.483192/1‑150 (MQ=60)
                                           GGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTA                                                                                                     <  SRR19973069.237740/150‑1 (MQ=60)
                                            GATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAA                                                                                                    >  SRR19973069.105204/1‑150 (MQ=60)
                                             ATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAA                                                                                                   >  SRR19973069.1096774/1‑150 (MQ=60)
                                               AAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGTTACGTAAATA                                                                                                 <  SRR19973069.3087902/150‑1 (MQ=60)
                                               AAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATA                                                                                                 >  SRR19973069.3534927/1‑150 (MQ=60)
                                               AAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATA                                                                                                 <  SRR19973069.1249873/150‑1 (MQ=60)
                                                AATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAA                                                                                                >  SRR19973069.242456/1‑150 (MQ=60)
                                                AATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAA                                                                                                >  SRR19973069.242512/1‑150 (MQ=60)
                                                 ATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAAT                                                                                               >  SRR19973069.1661240/1‑150 (MQ=60)
                                                 ATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAAT                                                                                               <  SRR19973069.1042675/150‑1 (MQ=60)
                                                 ATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAAT                                                                                               >  SRR19973069.865843/1‑150 (MQ=60)
                                                 ATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAAT                                                                                               >  SRR19973069.2067111/1‑150 (MQ=60)
                                                  TACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATG                                                                                              <  SRR19973069.3144059/150‑1 (MQ=60)
                                                   ACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGA                                                                                             >  SRR19973069.2191262/1‑150 (MQ=60)
                                                    CATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGAT                                                                                            >  SRR19973069.3490759/1‑150 (MQ=60)
                                                     ATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATT                                                                                           <  SRR19973069.2278300/150‑1 (MQ=60)
                                                        GCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGG                                                                                        <  SRR19973069.2129757/150‑1 (MQ=60)
                                                        GCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGG                                                                                        <  SRR19973069.3392016/150‑1 (MQ=60)
                                                         CAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGT                                                                                       >  SRR19973069.2280745/1‑150 (MQ=60)
                                                          AGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTG                                                                                      >  SRR19973069.555596/1‑150 (MQ=60)
                                                                  GCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGC                                                                              >  SRR19973069.820168/1‑150 (MQ=60)
                                                                  GCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGC                                                                              >  SRR19973069.3395369/1‑150 (MQ=60)
                                                                       GTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGAC                                                                         >  SRR19973069.1807621/1‑150 (MQ=60)
                                                                        TACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACT                                                                        >  SRR19973069.2154732/1‑150 (MQ=60)
                                                                        TACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACT                                                                        >  SRR19973069.2153377/1‑150 (MQ=60)
                                                                        TACCAAAACACCAGCCATCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACT                                                                        <  SRR19973069.3326221/150‑1 (MQ=60)
                                                                          CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                      >  SRR19973069.1245221/1‑150 (MQ=60)
                                                                          CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                      <  SRR19973069.107550/150‑1 (MQ=60)
                                                                          CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                      <  SRR19973069.107540/150‑1 (MQ=60)
                                                                          CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                      <  SRR19973069.2060084/150‑1 (MQ=60)
                                                                          CCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTG                                                                      >  SRR19973069.3161380/1‑150 (MQ=60)
                                                                           CAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCACTTGTACCCTTAACTCTTCCTGATACGTAAATAAAGATTTGGTGGCCCTAGCTGGACTTGA                                                                     >  SRR19973069.3504164/1‑150 (MQ=60)
                                                                            AAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAA                                                                    >  SRR19973069.3456026/1‑150 (MQ=60)
                                                                                 ACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGC                                                               >  SRR19973069.1039693/1‑150 (MQ=60)
                                                                                  CCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCG                                                              >  SRR19973069.2258140/1‑150 (MQ=60)
                                                                                  CCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCG                                                              >  SRR19973069.2960594/1‑150 (MQ=60)
                                                                                    AGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGAC                                                            >  SRR19973069.66063/1‑150 (MQ=60)
                                                                                       CCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAA                                                         >  SRR19973069.2817303/1‑150 (MQ=60)
                                                                                               GAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATATACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAT                                                 >  SRR19973069.2695727/1‑150 (MQ=60)
                                                                                               GAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAT                                                 <  SRR19973069.1991554/150‑1 (MQ=60)
                                                                                               GAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTAT                                                 <  SRR19973069.3252889/150‑1 (MQ=60)
                                                                                                AAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATG                                                >  SRR19973069.1277597/1‑150 (MQ=60)
                                                                                                  ACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAG                                              >  SRR19973069.3343579/1‑150 (MQ=60)
                                                                                                  ACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAG                                              >  SRR19973069.1427458/1‑150 (MQ=60)
                                                                                                  ACCACCAACCGCACTTCTTCCTTGAAATTGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAG                                              <  SRR19973069.3195822/150‑1 (MQ=60)
                                                                                                    CNCCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTC                                            >  SRR19973069.321821/1‑150 (MQ=60)
                                                                                                    CACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTC                                            >  SRR19973069.321881/1‑150 (MQ=60)
                                                                                                     ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                           <  SRR19973069.573571/150‑1 (MQ=60)
                                                                                                     ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                           <  SRR19973069.2894955/150‑1 (MQ=60)
                                                                                                     ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                           <  SRR19973069.712832/150‑1 (MQ=60)
                                                                                                     ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                           <  SRR19973069.1798658/150‑1 (MQ=60)
                                                                                                     ACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCG                                           <  SRR19973069.2500500/150‑1 (MQ=60)
                                                                                                      CCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGC                                          >  SRR19973069.3735422/1‑150 (MQ=60)
                                                                                                           CGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCT                                     >  SRR19973069.2421537/1‑150 (MQ=60)
                                                                                                             CACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCT                                   <  SRR19973069.3087663/150‑1 (MQ=60)
                                                                                                                 TCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACC                               <  SRR19973069.1089014/150‑1 (MQ=60)
                                                                                                                  CTTCCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCA                              >  SRR19973069.920026/1‑150 (MQ=60)
                                                                                                                     CCTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTG                           <  SRR19973069.2276657/150‑1 (MQ=60)
                                                                                                                      CTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGA                          >  SRR19973069.1830105/1‑150 (MQ=60)
                                                                                                                      CTTGAAATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGA                          >  SRR19973069.2783556/1‑150 (MQ=60)
                                                                                                                           AATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAA                     >  SRR19973069.600815/1‑150 (MQ=60)
                                                                                                                           AATGGCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACACTTCCTGATACGTAAAAAAAGATTTGGTGGCCCATGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGATCTAACCACTGAGCTAA                     >  SRR19973069.600741/1‑150 (MQ=60)
                                                                                                                               GCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGG                 >  SRR19973069.844430/1‑150 (MQ=60)
                                                                                                                               GCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGG                 <  SRR19973069.2277956/150‑1 (MQ=60)
                                                                                                                               GCGTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGG                 <  SRR19973069.3499258/150‑1 (MQ=60)
                                                                                                                                 GTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCC               >  SRR19973069.3589595/1‑150 (MQ=60)
                                                                                                                                 GTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCC               >  SRR19973069.1683628/1‑150 (MQ=60)
                                                                                                                                 GTTAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATCATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCC               >  SRR19973069.1684303/1‑150 (MQ=60)
                                                                                                                                  TGAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCT              <  SRR19973069.3700885/150‑1 (MQ=60)
                                                                                                                                   TAGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTT             <  SRR19973069.732498/150‑1 (MQ=60)
                                                                                                                                    AGTCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTG            >  SRR19973069.3030952/1‑150 (MQ=60)
                                                                                                                                      TCATGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAG          <  SRR19973069.167695/150‑1 (MQ=60)
                                                                                                                                         TGAAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGT       >  SRR19973069.626831/1‑150 (MQ=60)
                                                                                                                                           AAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGC     >  SRR19973069.2683328/1‑150 (MQ=60)
                                                                                                                                           AAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGC     >  SRR19973069.2683403/1‑150 (MQ=60)
                                                                                                                                           AAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGC     >  SRR19973069.3251456/1‑150 (MQ=60)
                                                                                                                                           AAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGC     >  SRR19973069.1832696/1‑150 (MQ=60)
                                                                                                                                           AAATATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGC     >  SRR19973069.3251726/1‑150 (MQ=60)
                                                                                                                                              TATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAT  <  SRR19973069.3832729/150‑1 (MQ=60)
                                                                                                                                              TATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAT  <  SRR19973069.114739/150‑1 (MQ=60)
                                                                                                                                              TATAGACAGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCAATGAGCTAAAGGGCCTTGAGTGTGCAAT  <  SRR19973069.1096215/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                              
CATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAGTACCCCTTGTACCCTTAACTCTTCCTGATACGTAAATAATGATTTGGTGGCCCTTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAAGGGCCTTGAGTGTGCAAT  >  CP009273/2778918‑2779209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑