Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,986,052 G→T 100% L200F (TTG→TTT)  ygeH → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,986,0520GT98.0% 648.6 / 2.8 200L200F (TTG→TTT) ygeHputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (104/92);  total (107/93)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

GGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGG  >  CP009273/2985906‑2986198
                                                                                                                                                  |                                                                                                                                                  
ggTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATc                                                                                                                                                 <  1:3268668/150‑1 (MQ=255)
 gTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCg                                                                                                                                                <  2:2564304/150‑1 (MQ=255)
       ataCCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTa                                                                                                                                          >  1:10057/1‑150 (MQ=255)
       ataCCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTa                                                                                                                                          <  1:2848161/150‑1 (MQ=255)
       ataCCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTa                                                                                                                                          >  2:4107258/1‑150 (MQ=255)
        taCCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTaa                                                                                                                                         <  2:1933499/150‑1 (MQ=255)
        taCCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTaa                                                                                                                                         <  1:2361422/150‑1 (MQ=255)
           cTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTaaaaa                                                                                                                                      <  1:4407498/150‑1 (MQ=255)
           cTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTaaaaa                                                                                                                                      >  1:2486188/1‑150 (MQ=255)
               ccGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCtt                                                                                                                                  >  1:2552569/1‑150 (MQ=255)
                 gATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCtttt                                                                                                                                <  2:4174656/150‑1 (MQ=255)
                 gATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCtttt                                                                                                                                <  2:4580403/150‑1 (MQ=255)
                  aTGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTc                                                                                                                               >  2:2278543/1‑150 (MQ=255)
                  aTGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTc                                                                                                                               >  2:3477779/1‑150 (MQ=255)
                  aTGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTc                                                                                                                               >  2:1810287/1‑150 (MQ=255)
                      cTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCa                                                                                                                           <  2:2326252/150‑1 (MQ=255)
                      cTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCa                                                                                                                           >  2:3404676/1‑150 (MQ=255)
                       tGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCAt                                                                                                                          >  2:2780441/1‑150 (MQ=255)
                        gCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATc                                                                                                                         <  2:3462853/150‑1 (MQ=255)
                         cGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCg                                                                                                                        <  2:4816041/150‑1 (MQ=255)
                         cGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCg                                                                                                                        >  1:1695730/1‑150 (MQ=255)
                          gACAAATTTTTGTAATGATCACATATCTCAAAATTCNTTCTTGAGCAGATTAAATCAAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAAACTTTTCGTAATCGc                                                                                                                       >  1:2678409/1‑150 (MQ=255)
                          gACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGc                                                                                                                       >  2:2364874/1‑150 (MQ=255)
                          gACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGc                                                                                                                       >  2:3121085/1‑150 (MQ=255)
                           aCAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGcc                                                                                                                      <  1:2564930/150‑1 (MQ=255)
                              aaTTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAAAACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGt                                                                                                                   <  2:3332924/150‑1 (MQ=255)
                                tttttGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTaa                                                                                                                 <  1:2780441/150‑1 (MQ=255)
                                 ttttGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAat                                                                                                                <  2:815762/150‑1 (MQ=255)
                                          gATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCt                                                                                                       <  1:1789672/150‑1 (MQ=255)
                                          gATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCt                                                                                                       <  2:4737149/150‑1 (MQ=255)
                                              acaTATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtg                                                                                                   <  1:3404676/150‑1 (MQ=255)
                                                atatCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtgat                                                                                                 >  1:581554/1‑150 (MQ=255)
                                                atatCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtgat                                                                                                 <  1:115403/150‑1 (MQ=255)
                                                 tatCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtgatg                                                                                                <  1:4604391/150‑1 (MQ=255)
                                                  atCTCAAAATTCATTCTTGAGCAGATTCAAGCCATATTATTTCGTTACAGGAAGAATAAAACAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATAATCTCCCTGTtgatga                                                                                               <  2:5149956/150‑1 (MQ=255)
                                                  atCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtgatga                                                                                               <  1:4211338/150‑1 (MQ=255)
                                                  atCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtgatga                                                                                               <  1:1637101/150‑1 (MQ=255)
                                                  atCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTtgatga                                                                                               <  1:4808449/150‑1 (MQ=255)
                                                     tcAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACt                                                                                            <  2:3003922/150‑1 (MQ=255)
                                                       aaaaTTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTAc                                                                                          >  2:1635097/1‑150 (MQ=255)
                                                        aaaTTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACa                                                                                         <  1:5001415/150‑1 (MQ=255)
                                                         aaTTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACat                                                                                        >  2:922696/1‑150 (MQ=255)
                                                          attcattcTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACata                                                                                       <  2:4101930/150‑1 (MQ=255)
                                                          attcattcTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACata                                                                                       <  1:2762775/150‑1 (MQ=255)
                                                           ttcattcTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACataa                                                                                      <  1:942340/150‑1 (MQ=255)
                                                           ttcattcTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACataa                                                                                      <  1:1857343/150‑1 (MQ=255)
                                                             cattcTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACataata                                                                                    >  1:3225309/1‑150 (MQ=255)
                                                              attcTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATAc                                                                                   >  1:2882576/1‑150 (MQ=255)
                                                                 cTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATc                                                                                <  2:2691344/150‑1 (MQ=255)
                                                                 cTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATc                                                                                >  2:3621118/1‑150 (MQ=255)
                                                                   tGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTAAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATcac                                                                              >  2:3720567/1‑150 (MQ=255)
                                                                   tGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTAAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATcac                                                                              >  2:3720479/1‑150 (MQ=255)
                                                                        aGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAAttt                                                                         <  1:2994625/150‑1 (MQ=255)
                                                                        aGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAAttt                                                                         <  2:3683338/150‑1 (MQ=255)
                                                                        aGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAAttt                                                                         <  2:3683191/150‑1 (MQ=255)
                                                                             cAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTtattat                                                                    >  1:474617/1‑150 (MQ=255)
                                                                              aaGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATtata                                                                   >  2:2483486/1‑150 (MQ=255)
                                                                              aaGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATtata                                                                   <  2:2727809/150‑1 (MQ=255)
                                                                              aaGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATtata                                                                   >  1:4518666/1‑150 (MQ=255)
                                                                                   aGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGataa                                                              >  1:1550984/1‑150 (MQ=255)
                                                                                    gATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGataat                                                             >  1:4581030/1‑150 (MQ=255)
                                                                                     attattTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGataata                                                            >  2:1137030/1‑150 (MQ=255)
                                                                                        attTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATcc                                                         <  2:1401073/150‑1 (MQ=255)
                                                                                         tttCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCt                                                        >  1:3509414/1‑150 (MQ=255)
                                                                                             gTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCaa                                                    >  1:3904452/1‑150 (MQ=255)
                                                                                             gTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCaa                                                    <  1:5121739/150‑1 (MQ=255)
                                                                                               tACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAc                                                  >  1:3922240/1‑150 (MQ=255)
                                                                                               tACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAc                                                  <  1:2731298/150‑1 (MQ=255)
                                                                                                aCAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACg                                                 >  1:4907301/1‑150 (MQ=255)
                                                                                                  aGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGt                                               <  1:2044884/150‑1 (MQ=255)
                                                                                                  aGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGt                                               <  1:1573714/150‑1 (MQ=255)
                                                                                                  aGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGt                                               >  1:4900251/1‑150 (MQ=255)
                                                                                                  aGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGt                                               >  1:3672712/1‑150 (MQ=255)
                                                                                                     aagaaTAAACCAGAATAATGCAGTAAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACa                                            >  1:3653008/1‑150 (MQ=255)
                                                                                                             aCCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAg                                    >  2:4429727/1‑150 (MQ=255)
                                                                                                              ccAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGa                                   >  1:538559/1‑150 (MQ=255)
                                                                                                               cAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGaa                                  <  1:3972119/150‑1 (MQ=255)
                                                                                                                aGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAc                                 >  1:5096777/1‑150 (MQ=255)
                                                                                                                aGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAc                                 >  2:497823/1‑150 (MQ=255)
                                                                                                                aGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAc                                 >  2:2108295/1‑150 (MQ=255)
                                                                                                                  aataatGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGa                               >  2:1897721/1‑150 (MQ=255)
                                                                                                                   ataatGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGAt                              <  2:2156362/150‑1 (MQ=255)
                                                                                                                     aatGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAAGATCt                            >  2:4712726/1‑150 (MQ=255)
                                                                                                                       tGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGt                          <  2:1642023/150‑1 (MQ=255)
                                                                                                                         cAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTaa                        <  2:4531637/150‑1 (MQ=255)
                                                                                                                           gTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGa                      >  1:4370624/1‑150 (MQ=255)
                                                                                                                              aaCACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTa                   <  1:2474254/150‑1 (MQ=255)
                                                                                                                              aaCACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTa                   <  1:3477779/150‑1 (MQ=255)
                                                                                                                                cacTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGc                 >  1:528893/1‑150 (MQ=255)
                                                                                                                                    ttATACATTGAGTTTATCGACGCTAAAAACCTTTTACTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAg             <  2:2552586/150‑1 (MQ=255)
                                                                                                                                      ataCATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCa           >  2:1201612/1‑150 (MQ=255)
                                                                                                                                      ataCATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCa           >  2:3972518/1‑150 (MQ=255)
                                                                                                                                       taCATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAg          <  1:3121085/150‑1 (MQ=255)
                                                                                                                                         cATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGa        >  1:220070/1‑150 (MQ=255)
                                                                                                                                         cATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGa        >  2:4929070/1‑150 (MQ=255)
                                                                                                                                          aTTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGAc       <  1:613768/150‑1 (MQ=255)
                                                                                                                                          aTTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGAc       <  2:2882576/150‑1 (MQ=255)
                                                                                                                                            tGAGTTTATCGACGCTAAAAACCTTTTCCTAATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCa     >  2:1244450/1‑150 (MQ=255)
                                                                                                                                              aGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAg   <  2:4581522/150‑1 (MQ=255)
                                                                                                                                               gTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAgg  <  1:644614/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGG  >  CP009273/2985906‑2986198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATA  >  CP009273/2985904‑2986201
                                                                                                                                                    |                                                                                                                                                     
ATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTA                                                                                                                                                      >  SRR19973063.3197491/1‑150 (MQ=60)
  GGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATC                                                                                                                                                    <  SRR19973063.3279158/150‑1 (MQ=60)
     CTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGAC                                                                                                                                                 >  SRR19973063.4596177/1‑150 (MQ=60)
       CTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGC                                                                                                                                               >  SRR19973063.1887955/1‑150 (MQ=60)
         ATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTA                                                                                                                                             >  SRR19973063.10099/1‑150 (MQ=60)
         ATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTA                                                                                                                                             <  SRR19973063.2857298/150‑1 (MQ=60)
         ATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTA                                                                                                                                             >  SRR19973063.9998/1‑150 (MQ=60)
          TACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAA                                                                                                                                            <  SRR19973063.2368987/150‑1 (MQ=60)
             CTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAA                                                                                                                                         >  SRR19973063.2494133/1‑150 (MQ=60)
             CTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAA                                                                                                                                         <  SRR19973063.4421634/150‑1 (MQ=60)
              TATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAAC                                                                                                                                        >  SRR19973063.2064420/1‑150 (MQ=60)
                TCCGATGGCTGCGACAAATTTTTGTAACGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCT                                                                                                                                      <  SRR19973063.3706674/150‑1 (MQ=60)
                 CCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTT                                                                                                                                     >  SRR19973063.2560720/1‑150 (MQ=60)
                 CCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTT                                                                                                                                     >  SRR19973063.2560703/1‑150 (MQ=60)
                  CGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTT                                                                                                                                    >  SRR19973063.2704607/1‑150 (MQ=60)
                   GATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAAatccag                                                                                                                                   >  SRR19973063.5143794/1‑144 (MQ=60)
                   GATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTT                                                                                                                                   >  SRR19973063.58090/1‑150 (MQ=60)
                     TGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCC                                                                                                                                 >  SRR19973063.2215436/1‑150 (MQ=60)
                           CGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCG                                                                                                                           >  SRR19973063.1700898/1‑150 (MQ=60)
                            GACAAATTTTTGTAATGATCACATATCTCAAAATTCNTTCTTGAGCAGATTAAATCAAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAAACTTTTCGTAATCGC                                                                                                                          >  SRR19973063.2687022/1‑150 (MQ=60)
                            GACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGC                                                                                                                          >  SRR19973063.1443027/1‑150 (MQ=60)
                             ACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCC                                                                                                                         <  SRR19973063.2573102/150‑1 (MQ=60)
                              CAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCA                                                                                                                        <  SRR19973063.5021227/150‑1 (MQ=60)
                                 ATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTA                                                                                                                     >  SRR19973063.3837324/1‑150 (MQ=60)
                                  TTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAA                                                                                                                    <  SRR19973063.2789395/150‑1 (MQ=60)
                                        TAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCT                                                                                                              <  SRR19973063.249446/150‑1 (MQ=60)
                                            GATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCT                                                                                                          <  SRR19973063.753724/150‑1 (MQ=60)
                                            GATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCT                                                                                                          <  SRR19973063.2388370/150‑1 (MQ=60)
                                            GATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCT                                                                                                          <  SRR19973063.1795210/150‑1 (MQ=60)
                                               CACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTT                                                                                                       <  SRR19973063.4773059/150‑1 (MQ=60)
                                               CACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTT                                                                                                       >  SRR19973063.3202237/1‑150 (MQ=60)
                                                ACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTG                                                                                                      <  SRR19973063.3415505/150‑1 (MQ=60)
                                                 CATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGA                                                                                                     >  SRR19973063.1841947/1‑150 (MQ=60)
                                                  ATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGAT                                                                                                    <  SRR19973063.4897471/150‑1 (MQ=60)
                                                  ATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGAT                                                                                                    >  SRR19973063.583272/1‑150 (MQ=60)
                                                  ATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGAT                                                                                                    >  SRR19973063.3252243/1‑150 (MQ=60)
                                                  ATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGAT                                                                                                    <  SRR19973063.115787/150‑1 (MQ=60)
                                                   TATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATG                                                                                                   <  SRR19973063.4619118/150‑1 (MQ=60)
                                                    ATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGA                                                                                                  <  SRR19973063.4823825/150‑1 (MQ=60)
                                                    ATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGA                                                                                                  <  SRR19973063.4224769/150‑1 (MQ=60)
                                                    ATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGA                                                                                                  <  SRR19973063.1642108/150‑1 (MQ=60)
                                                          AAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACA                                                                                            <  SRR19973063.3104515/150‑1 (MQ=60)
                                                          AAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACA                                                                                            <  SRR19973063.5017563/150‑1 (MQ=60)
                                                          AAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACA                                                                                            <  SRR19973063.5017511/150‑1 (MQ=60)
                                                            ATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATA                                                                                          <  SRR19973063.2771680/150‑1 (MQ=60)
                                                            ATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATA                                                                                          <  SRR19973063.4517245/150‑1 (MQ=60)
                                                             TTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAA                                                                                         <  SRR19973063.1863148/150‑1 (MQ=60)
                                                             TTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAA                                                                                         <  SRR19973063.945152/150‑1 (MQ=60)
                                                              TCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAAT                                                                                        >  SRR19973063.2837713/1‑150 (MQ=60)
                                                               CATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATA                                                                                       >  SRR19973063.3235671/1‑150 (MQ=60)
                                                                ATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATAC                                                                                      >  SRR19973063.2891791/1‑150 (MQ=60)
                                                                        GCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAAT                                                                              >  SRR19973063.643177/1‑150 (MQ=60)
                                                                         CAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATT                                                                             <  SRR19973063.4290493/150‑1 (MQ=60)
                                                                          AGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTT                                                                            >  SRR19973063.1033342/1‑150 (MQ=60)
                                                                          AGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTT                                                                            <  SRR19973063.3004181/150‑1 (MQ=60)
                                                                            ATTCAAGCCATATTATTTCGTTACAGGAAGAATAAACCATAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTAT                                                                          <  SRR19973063.4670430/150‑1 (MQ=60)
                                                                              TCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTA                                                                        >  SRR19973063.3595927/1‑150 (MQ=60)
                                                                               CAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTAT                                                                       >  SRR19973063.475960/1‑150 (MQ=60)
                                                                                AAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATA                                                                      >  SRR19973063.4390486/1‑150 (MQ=60)
                                                                                AAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATA                                                                      >  SRR19973063.4533167/1‑150 (MQ=60)
                                                                                    CAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATA                                                                  >  SRR19973063.5897/1‑150 (MQ=60)
                                                                                     AGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAA                                                                 >  SRR19973063.1555733/1‑150 (MQ=60)
                                                                                     AGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAA                                                                 >  SRR19973063.1556012/1‑150 (MQ=60)
                                                                                      GATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAAT                                                                >  SRR19973063.4595683/1‑150 (MQ=60)
                                                                                      GATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAAT                                                                >  SRR19973063.4595470/1‑150 (MQ=60)
                                                                                         TATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATC                                                             >  SRR19973063.278595/1‑150 (MQ=60)
                                                                                           TTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCT                                                           >  SRR19973063.3520637/1‑150 (MQ=60)
                                                                                               GTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAA                                                       <  SRR19973063.5138202/150‑1 (MQ=60)
                                                                                               GTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAA                                                       >  SRR19973063.3916893/1‑150 (MQ=60)
                                                                                               GTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAA                                                       <  SRR19973063.5138216/150‑1 (MQ=60)
                                                                                                 TACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAC                                                     >  SRR19973063.3932713/1‑150 (MQ=60)
                                                                                                 TACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAC                                                     >  SRR19973063.3934754/1‑150 (MQ=60)
                                                                                                 TACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAC                                                     <  SRR19973063.2740112/150‑1 (MQ=60)
                                                                                                 TACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAC                                                     <  SRR19973063.2740153/150‑1 (MQ=60)
                                                                                                  ACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACG                                                    <  SRR19973063.1608555/150‑1 (MQ=60)
                                                                                                  ACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACG                                                    >  SRR19973063.4923149/1‑150 (MQ=60)
                                                                                                   CAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAAAGG                                                   >  SRR19973063.171475/1‑150 (MQ=60)
                                                                                                    AGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGT                                                  >  SRR19973063.3684477/1‑150 (MQ=60)
                                                                                                    AGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGT                                                  <  SRR19973063.1578542/150‑1 (MQ=60)
                                                                                                    AGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGT                                                  >  SRR19973063.4916072/1‑150 (MQ=60)
                                                                                                    AGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGT                                                  <  SRR19973063.2051273/150‑1 (MQ=60)
                                                                                                    AGGAAGAATAAACCAGAATAATGAATTGAACACTTTATAAATTGAGATTATCGACGCTAAAAACCTTTTCATCAACGCCAGTAATCATCTCCCTGTTGATGAACTACATAATAAATCACAATTTATTATAGATAATATCCTTCAAAagtt                                                  >  SRR19973063.4916087/1‑146 (MQ=60)
                                                                                                     GGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTA                                                 <  SRR19973063.2881320/150‑1 (MQ=60)
                                                                                                      GAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTAC                                                >  SRR19973063.2492717/1‑150 (MQ=60)
                                                                                                       AAGAATAAACCAGAATAATGCAGTAAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACA                                               >  SRR19973063.3664720/1‑150 (MQ=60)
                                                                                                         GAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATA                                             >  SRR19973063.2903218/1‑150 (MQ=60)
                                                                                                             AAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACC                                         >  SRR19973063.3308600/1‑150 (MQ=60)
                                                                                                             AAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACC                                         >  SRR19973063.3982359/1‑150 (MQ=60)
                                                                                                              AACCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCA                                        >  SRR19973063.3493970/1‑150 (MQ=60)
                                                                                                                CCAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGA                                      >  SRR19973063.540150/1‑150 (MQ=60)
                                                                                                                 CAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAA                                     <  SRR19973063.3984835/150‑1 (MQ=60)
                                                                                                                 CAGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAA                                     >  SRR19973063.1644437/1‑150 (MQ=60)
                                                                                                                  AGAATAATGCAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAAC                                    >  SRR19973063.5113177/1‑150 (MQ=60)
                                                                                                                           CAGTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAA                           <  SRR19973063.2609449/150‑1 (MQ=60)
                                                                                                                             GTGAACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGA                         >  SRR19973063.4384607/1‑150 (MQ=60)
                                                                                                                                AACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTA                      <  SRR19973063.2482162/150‑1 (MQ=60)
                                                                                                                                AACACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTA                      <  SRR19973063.3488832/150‑1 (MQ=60)
                                                                                                                                AACACTTTATACATTGAGTTTATCGACGCTAAAAACCATTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTA                      <  SRR19973063.3488868/150‑1 (MQ=60)
                                                                                                                                  CACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGC                    >  SRR19973063.530444/1‑150 (MQ=60)
                                                                                                                                  CACTTTATACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGC                    >  SRR19973063.530464/1‑150 (MQ=60)
                                                                                                                                         TACATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAG             <  SRR19973063.3131132/150‑1 (MQ=60)
                                                                                                                                           CATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGA           >  SRR19973063.220731/1‑150 (MQ=60)
                                                                                                                                            ATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGAC          <  SRR19973063.615570/150‑1 (MQ=60)
                                                                                                                                            ATTGAGTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGAC          <  SRR19973063.4120412/150‑1 (MQ=60)
                                                                                                                                                 GTTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGG     <  SRR19973063.646504/150‑1 (MQ=60)
                                                                                                                                                  TTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGA    >  SRR19973063.3562524/1‑150 (MQ=60)
                                                                                                                                                  TTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGA    >  SRR19973063.973400/1‑150 (MQ=60)
                                                                                                                                                  TTTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGA    <  SRR19973063.1162408/150‑1 (MQ=60)
                                                                                                                                                   TTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGAT   <  SRR19973063.2285817/150‑1 (MQ=60)
                                                                                                                                                   TTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGAT   >  SRR19973063.272327/1‑150 (MQ=60)
                                                                                                                                                   TTATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGAT   <  SRR19973063.839445/150‑1 (MQ=60)
                                                                                                                                                    TATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATA  >  SRR19973063.2476196/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTCAAGCCAGATTATTTCGTTACAGGAAGAATAAACCAGAATAATGCAGTGAACACTTTATACATTGAGTTGATCGACGCTAAAAACCTTTTCCTCATCGCCAGTAATCATCTCCCTGTTGATGAACTACATAATACATCACAATTTATTATAGATAATATCCTTCAAACGGTACATAAACCAGAACGATCTGTAAGATTAGCTAAGCAGGACCAAGGATA  >  CP009273/2985904‑2986201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑