Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 400,594 G→T 100% V169V (GTC→GTA)  proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273400,5940GT96.5% 625.5 / 12.0 212V169V (GTC→GTA) proCpyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (3/5);  new base T (94/110);  total (97/115)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

TTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTT  >  CP009273/400449‑400740
                                                                                                                                                 |                                                                                                                                                  
tttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTac                                                                                                                                                  >  1:1592553/1‑148 (MQ=255)
 ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                                 <  1:1895152/148‑1 (MQ=255)
 ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                                 <  1:1833738/148‑1 (MQ=255)
    cAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTaccacc                                                                                                                                              <  2:424487/148‑1 (MQ=255)
      gCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCgg                                                                                                                                            <  1:2862459/148‑1 (MQ=255)
       cACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCggg                                                                                                                                           <  1:3051460/148‑1 (MQ=255)
        aCCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGt                                                                                                                                          >  2:2402110/1‑148 (MQ=255)
        aCCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGt                                                                                                                                          <  1:1298738/148‑1 (MQ=255)
        aCCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGt                                                                                                                                          >  2:2226561/1‑148 (MQ=255)
         ccATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTg                                                                                                                                         <  2:2441487/148‑1 (MQ=255)
          cATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTgg                                                                                                                                        >  1:3343714/1‑148 (MQ=255)
           aTTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGa                                                                                                                                       <  1:1153423/148‑1 (MQ=255)
             ttttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatc                                                                                                                                     >  2:3130548/1‑148 (MQ=255)
             ttttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatc                                                                                                                                     >  2:1614033/1‑148 (MQ=255)
              tttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatca                                                                                                                                    <  1:3256144/148‑1 (MQ=255)
                 gCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCg                                                                                                                                 <  1:2295892/148‑1 (MQ=255)
                   ggAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGc                                                                                                                               <  2:1435274/148‑1 (MQ=255)
                    gAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCt                                                                                                                              <  2:2780810/148‑1 (MQ=255)
                       cccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAg                                                                                                                           >  2:523587/1‑148 (MQ=255)
                         cATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCa                                                                                                                         >  2:400907/1‑148 (MQ=255)
                          aTTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCaa                                                                                                                        <  2:1273947/148‑1 (MQ=255)
                            tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                      >  2:2034400/1‑148 (MQ=255)
                            tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                      >  2:222593/1‑148 (MQ=255)
                              ccGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTAc                                                                                                                    <  2:2481536/148‑1 (MQ=255)
                              ccGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTAc                                                                                                                    <  1:2647771/148‑1 (MQ=255)
                                gCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACtt                                                                                                                  <  1:2204244/148‑1 (MQ=255)
                                 ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                                 <  1:2812313/148‑1 (MQ=255)
                                 ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                                 <  1:1511787/148‑1 (MQ=255)
                                  cTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTcc                                                                                                                >  1:1084199/1‑148 (MQ=255)
                                    gAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGc                                                                                                              <  2:1600037/148‑1 (MQ=255)
                                      gCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCtt                                                                                                            <  1:615383/148‑1 (MQ=255)
                                       cGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTc                                                                                                           >  2:2059515/1‑148 (MQ=255)
                                         gCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGc                                                                                                         >  1:4463/1‑148 (MQ=255)
                                         gCAAATTTATACGCCTGGGCCCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGc                                                                                                         >  1:394918/1‑148 (MQ=255)
                                          cAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGcc                                                                                                        >  1:890748/1‑148 (MQ=255)
                                            aaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaa                                                                                                      >  1:1034138/1‑148 (MQ=255)
                                             aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                     >  1:377061/1‑148 (MQ=255)
                                             aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                     >  2:2985826/1‑148 (MQ=255)
                                               ttATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagc                                                                                                   <  2:889776/148‑1 (MQ=255)
                                               ttATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagc                                                                                                   >  1:2521949/1‑148 (MQ=255)
                                               ttATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagc                                                                                                   <  1:1380980/148‑1 (MQ=255)
                                                tataCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagca                                                                                                  <  1:2443505/148‑1 (MQ=255)
                                                  taCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagcagc                                                                                                <  2:737863/148‑1 (MQ=255)
                                                   aCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCg                                                                                               >  1:2780124/1‑148 (MQ=255)
                                                   aCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCg                                                                                               >  1:995454/1‑148 (MQ=255)
                                                   aCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCg                                                                                               >  1:933430/1‑148 (MQ=255)
                                                      ccTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaa                                                                                            <  1:1566681/148‑1 (MQ=255)
                                                      ccTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaa                                                                                            >  2:1553225/1‑148 (MQ=255)
                                                        tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                          >  2:1697289/1‑148 (MQ=255)
                                                        tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                          <  1:1023395/148‑1 (MQ=255)
                                                        tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                          <  1:2494155/148‑1 (MQ=255)
                                                          ggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAata                                                                                        >  1:813826/1‑148 (MQ=255)
                                                          ggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAata                                                                                        >  1:372042/1‑148 (MQ=255)
                                                             gcgTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTc                                                                                     <  1:3322579/148‑1 (MQ=255)
                                                               gTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAg                                                                                   <  1:2142678/148‑1 (MQ=255)
                                                                  gCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGcac                                                                                <  2:2702344/148‑1 (MQ=255)
                                                                   cATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGcaca                                                                               >  1:318168/1‑148 (MQ=255)
                                                                      cccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                            >  1:2786976/1‑148 (MQ=255)
                                                                      cccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                            <  1:1731154/148‑1 (MQ=255)
                                                                       ccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAg                                                                           <  2:192544/148‑1 (MQ=255)
                                                                           ccAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGt                                                                       <  1:2243278/148‑1 (MQ=255)
                                                                             aGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAt                                                                     <  2:820008/148‑1 (MQ=255)
                                                                                   gcggcgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTg                                                               <  1:1360907/148‑1 (MQ=255)
                                                                                          cGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTAc                                                        <  1:198814/148‑1 (MQ=255)
                                                                                             ccATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAg                                                     >  1:1009593/1‑148 (MQ=255)
                                                                                              cATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgc                                                    <  1:401271/148‑1 (MQ=255)
                                                                                               aTCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgcg                                                   >  2:3470407/1‑148 (MQ=255)
                                                                                               aTCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgcg                                                   <  2:2904220/148‑1 (MQ=255)
                                                                                               aTCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAgcg                                                   >  1:2378576/1‑148 (MQ=255)
                                                                                                   cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCGAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                               <  2:1009039/148‑1 (MQ=255)
                                                                                                   cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                               >  1:3112080/1‑148 (MQ=255)
                                                                                                   cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                               >  1:704941/1‑148 (MQ=255)
                                                                                                     tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                             >  2:2287453/1‑148 (MQ=255)
                                                                                                        aTAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCg                                          <  1:837650/148‑1 (MQ=255)
                                                                                                         tAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGt                                         >  1:1568544/1‑148 (MQ=255)
                                                                                                         tAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGt                                         >  2:938147/1‑148 (MQ=255)
                                                                                                            aCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTAc                                      >  1:864578/1‑148 (MQ=255)
                                                                                                             cATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACg                                     >  1:1229402/1‑148 (MQ=255)
                                                                                                              aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                    <  1:2268996/148‑1 (MQ=255)
                                                                                                                    aCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCa                              <  2:2774267/148‑1 (MQ=255)
                                                                                                                      gTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATc                            <  2:2144462/148‑1 (MQ=255)
                                                                                                                      gTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATc                            <  2:377061/148‑1 (MQ=255)
                                                                                                                       tAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                           <  2:258549/148‑1 (MQ=255)
                                                                                                                           cTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                       <  2:2242590/148‑1 (MQ=255)
                                                                                                                            tGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCa                      <  1:3195429/148‑1 (MQ=255)
                                                                                                                               cGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTa                   <  1:508651/148‑1 (MQ=255)
                                                                                                                                gaagaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTaa                  <  1:2526543/148‑1 (MQ=255)
                                                                                                                                  agaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAcc                <  1:3465700/148‑1 (MQ=255)
                                                                                                                                  agaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAcc                <  2:253407/148‑1 (MQ=255)
                                                                                                                                   gaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCa               >  2:3457925/1‑148 (MQ=255)
                                                                                                                                   gaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCa               >  2:810542/1‑148 (MQ=255)
                                                                                                                                   gaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCa               <  2:863051/148‑1 (MQ=255)
                                                                                                                                    aaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAg              >  2:1103060/1‑148 (MQ=255)
                                                                                                                                     aCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGt             >  1:2946494/1‑148 (MQ=255)
                                                                                                                                     aCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGt             >  2:659076/1‑148 (MQ=255)
                                                                                                                                      ccGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCATAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTg            <  2:3343714/148‑1 (MQ=255)
                                                                                                                                      ccGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTg            <  2:2446689/148‑1 (MQ=255)
                                                                                                                                          tCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCggg        <  1:2282601/148‑1 (MQ=255)
                                                                                                                                           cacaCCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa       <  1:2034400/148‑1 (MQ=255)
                                                                                                                                            acacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAg      >  1:381762/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTT  >  CP009273/400449‑400740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCG  >  CP009273/400447‑400742
                                                                                                                                                   |                                                                                                                                                    
CGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTAC                                                                                                                                                    >  SRR19973065.1603192/1‑150 (MQ=60)
   TTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCAC                                                                                                                                                 <  SRR19973065.1907605/150‑1 (MQ=60)
   TTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCAC                                                                                                                                                 <  SRR19973065.1845827/150‑1 (MQ=60)
    TCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACC                                                                                                                                                >  SRR19973065.2443791/1‑150 (MQ=60)
        GCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGT                                                                                                                                            <  SRR19973065.2880031/150‑1 (MQ=60)
         CACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTG                                                                                                                                           <  SRR19973065.3070120/150‑1 (MQ=60)
          ACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGG                                                                                                                                          >  SRR19973065.3364144/1‑150 (MQ=60)
          ACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGG                                                                                                                                          <  SRR19973065.1307308/150‑1 (MQ=60)
          ACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGG                                                                                                                                          <  SRR19973065.1307295/150‑1 (MQ=60)
           CCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGA                                                                                                                                         <  SRR19973065.2294579/150‑1 (MQ=60)
             ATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATC                                                                                                                                       <  SRR19973065.1160839/150‑1 (MQ=60)
                TTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATC                                                                                                                                    <  SRR19973065.3275841/150‑1 (MQ=60)
                   GCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGC                                                                                                                                 <  SRR19973065.2310349/150‑1 (MQ=60)
                          CCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAA                                                                                                                          >  SRR19973065.2508819/1‑150 (MQ=60)
                           CATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAT                                                                                                                         <  SRR19973065.410196/150‑1 (MQ=60)
                                CCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTT                                                                                                                    <  SRR19973065.2664092/150‑1 (MQ=60)
                                 CGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTC                                                                                                                   <  SRR19973065.2302733/150‑1 (MQ=60)
                                  GCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCC                                                                                                                  >  SRR19973065.1091353/1‑150 (MQ=60)
                                  GCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCC                                                                                                                  <  SRR19973065.2218349/150‑1 (MQ=60)
                                  GCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCC                                                                                                                  >  SRR19973065.1091417/1‑150 (MQ=60)
                                  GCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCC                                                                                                                  >  SRR19973065.2853181/1‑150 (MQ=60)
                                   CCTGAGCGGCATATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.502220/150‑1 (MQ=60)
                                   CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.2015689/150‑1 (MQ=60)
                                   CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.190473/150‑1 (MQ=60)
                                   CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.2828780/150‑1 (MQ=60)
                                   CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.2829700/150‑1 (MQ=60)
                                   CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.591503/150‑1 (MQ=60)
                                   CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                 <  SRR19973065.1521638/150‑1 (MQ=60)
                                      GAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTT                                                                                                              <  SRR19973065.1590336/150‑1 (MQ=60)
                                       AGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTC                                                                                                             >  SRR19973065.148900/1‑150 (MQ=60)
                                        GCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCG                                                                                                            <  SRR19973065.619328/150‑1 (MQ=60)
                                         CGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGC                                                                                                           >  SRR19973065.4522/1‑150 (MQ=60)
                                         CGGCAAATTTATACGCCTGGGCCCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGC                                                                                                           >  SRR19973065.397679/1‑150 (MQ=60)
                                          GGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCC                                                                                                          >  SRR19973065.896397/1‑150 (MQ=60)
                                          GGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCC                                                                                                          <  SRR19973065.2413706/150‑1 (MQ=60)
                                            CAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAA                                                                                                        >  SRR19973065.1040983/1‑150 (MQ=60)
                                            CAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAA                                                                                                        >  SRR19973065.3344161/1‑150 (MQ=60)
                                            CAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAA                                                                                                        >  SRR19973065.2261740/1‑150 (MQ=60)
                                             AAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAA                                                                                                       >  SRR19973065.379688/1‑150 (MQ=60)
                                             AAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAA                                                                                                       >  SRR19973065.2158254/1‑150 (MQ=60)
                                             AAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAA                                                                                                       >  SRR19973065.379684/1‑150 (MQ=60)
                                               ATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGC                                                                                                     >  SRR19973065.1688433/1‑150 (MQ=60)
                                               ATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGC                                                                                                     >  SRR19973065.2537786/1‑150 (MQ=60)
                                                 TTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAG                                                                                                   <  SRR19973065.1390072/150‑1 (MQ=60)
                                                  TATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGC                                                                                                  <  SRR19973065.2458904/150‑1 (MQ=60)
                                                  TATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGC                                                                                                  <  SRR19973065.384984/150‑1 (MQ=60)
                                                   ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                 >  SRR19973065.939592/1‑150 (MQ=60)
                                                   ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                 >  SRR19973065.2797375/1‑150 (MQ=60)
                                                   ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                 >  SRR19973065.2797578/1‑150 (MQ=60)
                                                   ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                 >  SRR19973065.1002183/1‑150 (MQ=60)
                                                   ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                 >  SRR19973065.1002070/1‑150 (MQ=60)
                                                   ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGGGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                 >  SRR19973065.1034057/1‑150 (MQ=60)
                                                      CGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAA                                                                                              >  SRR19973065.3225574/1‑150 (MQ=60)
                                                      CGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAA                                                                                              >  SRR19973065.176595/1‑150 (MQ=60)
                                                        CCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAA                                                                                            <  SRR19973065.1577095/150‑1 (MQ=60)
                                                        CCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAA                                                                                            <  SRR19973065.1577117/150‑1 (MQ=60)
                                                          TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGCACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                          <  SRR19973065.825617/150‑1 (MQ=60)
                                                          TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                          >  SRR19973065.819192/1‑150 (MQ=60)
                                                          TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                          <  SRR19973065.1029725/150‑1 (MQ=60)
                                                          TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                          <  SRR19973065.1030173/150‑1 (MQ=60)
                                                          TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                          >  SRR19973065.374643/1‑150 (MQ=60)
                                                          TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                          <  SRR19973065.2509848/150‑1 (MQ=60)
                                                               GCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAG                                                                                     <  SRR19973065.3342849/150‑1 (MQ=60)
                                                                 GTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCA                                                                                   <  SRR19973065.999612/150‑1 (MQ=60)
                                                                 GTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCA                                                                                   <  SRR19973065.1463908/150‑1 (MQ=60)
                                                                 GTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCA                                                                                   <  SRR19973065.2156461/150‑1 (MQ=60)
                                                                   GGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACA                                                                                 >  SRR19973065.2602442/1‑150 (MQ=60)
                                                                   GGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACA                                                                                 >  SRR19973065.320516/1‑150 (MQ=60)
                                                                   GGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACA                                                                                 >  SRR19973065.320354/1‑150 (MQ=60)
                                                                      ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                              <  SRR19973065.736605/150‑1 (MQ=60)
                                                                      ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                              >  SRR19973065.2804257/1‑150 (MQ=60)
                                                                      ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                              >  SRR19973065.849710/1‑150 (MQ=60)
                                                                        CCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGC                                                                            <  SRR19973065.1742616/150‑1 (MQ=60)
                                                                         CCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCG                                                                           <  SRR19973065.1566056/150‑1 (MQ=60)
                                                                             CCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAT                                                                       <  SRR19973065.2257518/150‑1 (MQ=60)
                                                                             CCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAT                                                                       <  SRR19973065.2257528/150‑1 (MQ=60)
                                                                              CAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATC                                                                      >  SRR19973065.3100499/1‑150 (MQ=60)
                                                                                  ACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCT                                                                  <  SRR19973065.1207044/150‑1 (MQ=60)
                                                                                   CGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTG                                                                 <  SRR19973065.1075517/150‑1 (MQ=60)
                                                                                     GCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGG                                                               <  SRR19973065.1369917/150‑1 (MQ=60)
                                                                                     GCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGG                                                               <  SRR19973065.1371135/150‑1 (MQ=60)
                                                                                         CGTCGGCCATCGCTTCGATAAACATAAAGACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTA                                                           <  SRR19973065.2533884/150‑1 (MQ=60)
                                                                                           TCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACC                                                         <  SRR19973065.2014873/150‑1 (MQ=60)
                                                                                            CGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCA                                                        <  SRR19973065.200356/150‑1 (MQ=60)
                                                                                            CGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCA                                                        <  SRR19973065.196980/150‑1 (MQ=60)
                                                                                             GGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAG                                                       >  SRR19973065.1239373/1‑150 (MQ=60)
                                                                                             GGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAG                                                       >  SRR19973065.1016287/1‑150 (MQ=60)
                                                                                             GGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAG                                                       <  SRR19973065.3396896/150‑1 (MQ=60)
                                                                                              GCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGC                                                      >  SRR19973065.554525/1‑150 (MQ=60)
                                                                                              GCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGC                                                      >  SRR19973065.2330489/1‑150 (MQ=60)
                                                                                               CCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCG                                                     >  SRR19973065.2393452/1‑150 (MQ=60)
                                                                                                CATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGC                                                    <  SRR19973065.404084/150‑1 (MQ=60)
                                                                                                CATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGC                                                    <  SRR19973065.403717/150‑1 (MQ=60)
                                                                                                 ATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCG                                                   >  SRR19973065.2689767/1‑150 (MQ=60)
                                                                                                   CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                 >  SRR19973065.709634/1‑150 (MQ=60)
                                                                                                   CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                 >  SRR19973065.3131048/1‑150 (MQ=60)
                                                                                                   CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTAACACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                 >  SRR19973065.708259/1‑150 (MQ=60)
                                                                                                       TCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGC                                             <  SRR19973065.3149598/150‑1 (MQ=60)
                                                                                                        CGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCG                                            >  SRR19973065.2948592/1‑150 (MQ=60)
                                                                                                         GATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGT                                           >  SRR19973065.3327533/1‑150 (MQ=60)
                                                                                                         GATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGT                                           >  SRR19973065.1578976/1‑150 (MQ=60)
                                                                                                          ATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTT                                          <  SRR19973065.843107/150‑1 (MQ=60)
                                                                                                            AAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTAC                                        >  SRR19973065.870137/1‑150 (MQ=60)
                                                                                                             AACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACG                                       >  SRR19973065.1237439/1‑150 (MQ=60)
                                                                                                              ACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGG                                      >  SRR19973065.2114700/1‑150 (MQ=60)
                                                                                                                ATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAG                                    <  SRR19973065.2283326/150‑1 (MQ=60)
                                                                                                                ATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCACAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAG                                    >  SRR19973065.888054/1‑150 (MQ=60)
                                                                                                                              TGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATT                      <  SRR19973065.3214688/150‑1 (MQ=60)
                                                                                                                                 CGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAC                   <  SRR19973065.512031/150‑1 (MQ=60)
                                                                                                                                  GAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAACGCAGCGGAAAATATTCAGCACATCCGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACC                  <  SRR19973065.2937621/150‑1 (MQ=60)
                                                                                                                                  GAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACC                  <  SRR19973065.2542129/150‑1 (MQ=60)
                                                                                                                                  GAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACC                  <  SRR19973065.2542406/150‑1 (MQ=60)
                                                                                                                                    AGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAG                <  SRR19973065.3486806/150‑1 (MQ=60)
                                                                                                                                    AGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAG                <  SRR19973065.3487647/150‑1 (MQ=60)
                                                                                                                                     GAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGT               >  SRR19973065.2964414/1‑150 (MQ=60)
                                                                                                                                       ACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGC             >  SRR19973065.3397359/1‑150 (MQ=60)
                                                                                                                                         CGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGG           >  SRR19973065.2969886/1‑150 (MQ=60)
                                                                                                                                            TCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG        >  SRR19973065.384432/1‑150 (MQ=60)
                                                                                                                                            TCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG        <  SRR19973065.906513/150‑1 (MQ=60)
                                                                                                                                            TCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG        <  SRR19973065.2296995/150‑1 (MQ=60)
                                                                                                                                             CACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGANT       <  SRR19973065.2047446/150‑1 (MQ=60)
                                                                                                                                                 CCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTC   <  SRR19973065.2014528/150‑1 (MQ=60)
                                                                                                                                                 CCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTC   <  SRR19973065.1718874/150‑1 (MQ=60)
                                                                                                                                                 CCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTC   <  SRR19973065.1718947/150‑1 (MQ=60)
                                                                                                                                                  CGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCG  <  SRR19973065.381138/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCG  >  CP009273/400447‑400742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑