Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 400,594 G→T 100% V169V (GTC→GTA)  proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273400,5940GT98.8% 562.0 / ‑1.7 173V169V (GTC→GTA) proCpyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (82/89);  total (82/91)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 173 total aligned reads displayed.

TTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG  >  CP009273/400450‑400736
                                                                                                                                                |                                                                                                                                              
ttCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTacc                                                                                                                                             >  2:1215952/1‑148 (MQ=255)
    aGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCg                                                                                                                                         <  1:1694132/148‑1 (MQ=255)
     gCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCgg                                                                                                                                        >  1:1362636/1‑148 (MQ=255)
     gCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCgg                                                                                                                                        >  1:340186/1‑148 (MQ=255)
       aCCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGt                                                                                                                                      <  1:2985621/148‑1 (MQ=255)
            ttttGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGatc                                                                                                                                 <  1:1257967/148‑1 (MQ=255)
                      cccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAg                                                                                                                       <  1:3336654/148‑1 (MQ=255)
                       ccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGc                                                                                                                      >  2:1604030/1‑148 (MQ=255)
                       ccATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGc                                                                                                                      <  1:2648872/148‑1 (MQ=255)
                        cATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCa                                                                                                                     >  1:2538324/1‑148 (MQ=255)
                        cATTACCGCCTGAGCGGCAAATCTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCa                                                                                                                     <  2:2756402/148‑1 (MQ=255)
                         aTTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCaa                                                                                                                    <  2:2845686/148‑1 (MQ=255)
                         aTTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCaa                                                                                                                    <  2:3571974/148‑1 (MQ=255)
                          ttACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAt                                                                                                                   <  1:3595828/148‑1 (MQ=255)
                          ttACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAt                                                                                                                   <  1:3595933/148‑1 (MQ=255)
                           tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                  >  2:581522/1‑148 (MQ=255)
                           tACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAAtt                                                                                                                  >  2:2060191/1‑148 (MQ=255)
                             ccGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTAc                                                                                                                >  2:3255928/1‑148 (MQ=255)
                                ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAACTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                             <  1:1948243/148‑1 (MQ=255)
                                ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                             <  1:1948484/148‑1 (MQ=255)
                                ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                             <  1:16178/148‑1 (MQ=255)
                                ccTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTc                                                                                                             <  2:1801765/148‑1 (MQ=255)
                                 cTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATCAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTcc                                                                                                            >  2:485451/1‑148 (MQ=255)
                                   gAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGc                                                                                                          >  1:2164183/1‑148 (MQ=255)
                                     gCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCtt                                                                                                        <  1:2607269/148‑1 (MQ=255)
                                          aaaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCa                                                                                                   >  2:392692/1‑148 (MQ=255)
                                           aaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaa                                                                                                  <  2:184792/148‑1 (MQ=255)
                                           aaTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaa                                                                                                  <  2:2890886/148‑1 (MQ=255)
                                            aTTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCaaa                                                                                                 >  2:1679018/1‑148 (MQ=255)
                                                ataCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAagcag                                                                                             <  1:1047558/148‑1 (MQ=255)
                                                  aCGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCg                                                                                           <  2:2347621/148‑1 (MQ=255)
                                                       tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                      <  1:2894959/148‑1 (MQ=255)
                                                       tGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGaaaa                                                                                      <  1:2186467/148‑1 (MQ=255)
                                                        gggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAat                                                                                     <  1:2270395/148‑1 (MQ=255)
                                                        gggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAat                                                                                     >  1:3392628/1‑148 (MQ=255)
                                                         ggCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAata                                                                                    <  1:1000612/148‑1 (MQ=255)
                                                               tGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGc                                                                              <  1:905604/148‑1 (MQ=255)
                                                                ggCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGca                                                                             >  2:1248847/1‑148 (MQ=255)
                                                                ggCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCATCGATAAACATAAATACGAAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTACGCTTCGCCAAACCAGCGGAAAATATTCAGca                                                                             >  1:2134494/1‑148 (MQ=255)
                                                                     cccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                        >  1:3752627/1‑148 (MQ=255)
                                                                     cccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                        >  1:3752616/1‑148 (MQ=255)
                                                                     cccGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCa                                                                        >  1:3188933/1‑148 (MQ=255)
                                                                       cGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGc                                                                      <  1:3797469/148‑1 (MQ=255)
                                                                        gCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCg                                                                     <  1:3703831/148‑1 (MQ=255)
                                                                        gCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCg                                                                     <  1:2964443/148‑1 (MQ=255)
                                                                           cAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTa                                                                  <  1:939609/148‑1 (MQ=255)
                                                                            aGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAt                                                                 <  1:1306764/148‑1 (MQ=255)
                                                                             gCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtc                                                                <  2:1065966/148‑1 (MQ=255)
                                                                             gCACGGCGGCGTCGGCCATCGCTTCGATAAACAGAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtc                                                                <  2:1066558/148‑1 (MQ=255)
                                                                              cACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCCACGCCGCGGAAAATATTCAGCTCATCAGCGGTAtct                                                               >  2:3526328/1‑148 (MQ=255)
                                                                              cACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtct                                                               >  2:2929598/1‑148 (MQ=255)
                                                                              cACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtct                                                               >  2:3527603/1‑148 (MQ=255)
                                                                              cACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtct                                                               >  2:3527676/1‑148 (MQ=255)
                                                                               aCGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtctt                                                              <  2:3048156/148‑1 (MQ=255)
                                                                                cggcggCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAtcttc                                                             <  2:1009023/148‑1 (MQ=255)
                                                                                   cggcgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTgg                                                          >  2:3429194/1‑148 (MQ=255)
                                                                                     gcgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTgggg                                                        <  2:3210860/148‑1 (MQ=255)
                                                                                     gcgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTgggg                                                        <  1:2600603/148‑1 (MQ=255)
                                                                                      cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGt                                                       <  2:3410555/148‑1 (MQ=255)
                                                                                      cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGt                                                       <  2:3095385/148‑1 (MQ=255)
                                                                                      cgTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGt                                                       >  1:141879/1‑148 (MQ=255)
                                                                                            ccATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAg                                                 >  2:2540946/1‑148 (MQ=255)
                                                                                                 gCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGt                                            <  1:2666849/148‑1 (MQ=255)
                                                                                                 gCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGt                                            <  1:2667327/148‑1 (MQ=255)
                                                                                                  cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                           >  1:3442311/1‑148 (MQ=255)
                                                                                                  cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                           >  1:2410678/1‑148 (MQ=255)
                                                                                                  cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                           >  1:2376314/1‑148 (MQ=255)
                                                                                                  cTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGtt                                           >  1:1760525/1‑148 (MQ=255)
                                                                                                    tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGACAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                         >  2:3613466/1‑148 (MQ=255)
                                                                                                    tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                         >  1:735387/1‑148 (MQ=255)
                                                                                                    tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                         >  1:1261103/1‑148 (MQ=255)
                                                                                                    tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTg                                         >  1:1716236/1‑148 (MQ=255)
                                                                                                    tCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGAACATCAGCGGTATCTTCTGGGGTTACCAGAGCGTTTg                                         >  1:3302491/1‑148 (MQ=255)
                                                                                                       aTAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCg                                      >  1:239393/1‑148 (MQ=255)
                                                                                                        tAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGt                                     >  2:757322/1‑148 (MQ=255)
                                                                                                         aaaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGtt                                    >  2:2405456/1‑148 (MQ=255)
                                                                                                          aaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTa                                   >  1:3182985/1‑148 (MQ=255)
                                                                                                             aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                <  1:485451/148‑1 (MQ=255)
                                                                                                             aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                >  1:3073349/1‑148 (MQ=255)
                                                                                                             aTAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACgg                                >  1:1168452/1‑148 (MQ=255)
                                                                                                                 aTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGt                            >  1:925929/1‑148 (MQ=255)
                                                                                                                 aTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGt                            >  1:924995/1‑148 (MQ=255)
                                                                                                                   aCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCa                          >  1:2818589/1‑148 (MQ=255)
                                                                                                                   aCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCa                          <  2:633494/148‑1 (MQ=255)
                                                                                                                   aCGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCa                          >  1:2992814/1‑148 (MQ=255)
                                                                                                                          cTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                   <  1:1840435/148‑1 (MQ=255)
                                                                                                                          cTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                   <  1:1838945/148‑1 (MQ=255)
                                                                                                                           tGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCa                  <  2:3716377/148‑1 (MQ=255)
                                                                                                                             gCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAtt                >  1:2507238/1‑148 (MQ=255)
                                                                                                                              cGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTa               <  1:823845/148‑1 (MQ=255)
                                                                                                                                aagaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAc             >  1:1499191/1‑148 (MQ=255)
                                                                                                                                 agaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAcc            <  1:299093/148‑1 (MQ=255)
                                                                                                                                  gaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCa           >  2:1809861/1‑148 (MQ=255)
                                                                                                                                  gaaCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGACATTAACCa           >  1:566350/1‑148 (MQ=255)
                                                                                                                                    aCCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGt         <  1:1409189/148‑1 (MQ=255)
                                                                                                                                     ccGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTg        <  2:989525/148‑1 (MQ=255)
                                                                                                                                        cTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCgg     >  2:2179421/1‑148 (MQ=255)
                                                                                                                                         tCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCggg    <  1:3638247/148‑1 (MQ=255)
                                                                                                                                           acacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAg  >  2:335008/1‑148 (MQ=255)
                                                                                                                                           acacCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAg  >  1:2698832/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG  >  CP009273/400450‑400736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTC  >  CP009273/400445‑400741
                                                                                                                                                     |                                                                                                                                                   
CCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCT                                                                                                                                                     >  SRR19973069.2375281/1‑150 (MQ=60)
CCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCT                                                                                                                                                     >  SRR19973069.436771/1‑150 (MQ=60)
CCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCT                                                                                                                                                     >  SRR19973069.436767/1‑150 (MQ=60)
  CGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTAC                                                                                                                                                   <  SRR19973069.444852/150‑1 (MQ=60)
        CAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGG                                                                                                                                             >  SRR19973069.342574/1‑150 (MQ=60)
        CAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGG                                                                                                                                             >  SRR19973069.1372112/1‑150 (MQ=60)
        CAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGG                                                                                                                                             >  SRR19973069.178784/1‑150 (MQ=60)
         AGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGG                                                                                                                                            <  SRR19973069.1705969/150‑1 (MQ=60)
           CACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTG                                                                                                                                          <  SRR19973069.3406791/150‑1 (MQ=60)
           CACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTG                                                                                                                                          <  SRR19973069.1491346/150‑1 (MQ=60)
            ACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGG                                                                                                                                         <  SRR19973069.3005450/150‑1 (MQ=60)
              CATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGAT                                                                                                                                       <  SRR19973069.3445972/150‑1 (MQ=60)
               ATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATC                                                                                                                                      >  SRR19973069.3740645/1‑150 (MQ=60)
                 TTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCAT                                                                                                                                    <  SRR19973069.1266459/150‑1 (MQ=60)
                           CCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCA                                                                                                                          >  SRR19973069.302733/1‑150 (MQ=60)
                           CCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCA                                                                                                                          <  SRR19973069.3358503/150‑1 (MQ=60)
                           CCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCA                                                                                                                          >  SRR19973069.2555132/1‑150 (MQ=60)
                            CCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAA                                                                                                                         <  SRR19973069.2666614/150‑1 (MQ=60)
                              ATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATT                                                                                                                       >  SRR19973069.2192373/1‑150 (MQ=60)
                               TTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTA                                                                                                                      <  SRR19973069.3619305/150‑1 (MQ=60)
                               TTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTA                                                                                                                      <  SRR19973069.3619409/150‑1 (MQ=60)
                               TTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTA                                                                                                                      <  SRR19973069.3352587/150‑1 (MQ=60)
                               TTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTA                                                                                                                      <  SRR19973069.3619412/150‑1 (MQ=60)
                                 ACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACT                                                                                                                    <  SRR19973069.3784973/150‑1 (MQ=60)
                                  CCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTT                                                                                                                   <  SRR19973069.2772793/150‑1 (MQ=60)
                                    GCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCC                                                                                                                 <  SRR19973069.1743103/150‑1 (MQ=60)
                                    GCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTCCC                                                                                                                 <  SRR19973069.194580/150‑1 (MQ=60)
                                     CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAACTACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                <  SRR19973069.1961703/150‑1 (MQ=60)
                                     CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                <  SRR19973069.16406/150‑1 (MQ=60)
                                     CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                <  SRR19973069.1961900/150‑1 (MQ=60)
                                     CCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCG                                                                                                                <  SRR19973069.1961946/150‑1 (MQ=60)
                                      CTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGC                                                                                                               >  SRR19973069.2178979/1‑150 (MQ=60)
                                        GAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTT                                                                                                             <  SRR19973069.3524387/150‑1 (MQ=60)
                                          GCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCG                                                                                                           <  SRR19973069.915409/150‑1 (MQ=60)
                                          GCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCG                                                                                                           <  SRR19973069.2624688/150‑1 (MQ=60)
                                          GCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCG                                                                                                           <  SRR19973069.2624639/150‑1 (MQ=60)
                                          GCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCG                                                                                                           <  SRR19973069.991932/150‑1 (MQ=60)
                                              CAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAA                                                                                                       >  SRR19973069.2577963/1‑150 (MQ=60)
                                                     ATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCG                                                                                                <  SRR19973069.1054831/150‑1 (MQ=60)
                                                        CGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAA                                                                                             >  SRR19973069.2089394/1‑150 (MQ=60)
                                                           CTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAAT                                                                                          >  SRR19973069.3414780/1‑150 (MQ=60)
                                                            TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                         <  SRR19973069.2914056/150‑1 (MQ=60)
                                                            TGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATA                                                                                         <  SRR19973069.2201357/150‑1 (MQ=60)
                                                             GGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATAT                                                                                        <  SRR19973069.2285791/150‑1 (MQ=60)
                                                              GGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATT                                                                                       <  SRR19973069.1007472/150‑1 (MQ=60)
                                                               GCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTC                                                                                      <  SRR19973069.167725/150‑1 (MQ=60)
                                                                CGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCA                                                                                     <  SRR19973069.3683621/150‑1 (MQ=60)
                                                                CGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGATTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCA                                                                                     <  SRR19973069.1249382/150‑1 (MQ=60)
                                                                   GTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCATCGATAAACATAAATACGAAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTACGCTTCGCCAAACCAGCGGAAAATATTCAGCA                                                                                  >  SRR19973069.2149167/1‑150 (MQ=60)
                                                                    TGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCAC                                                                                 <  SRR19973069.911816/150‑1 (MQ=60)
                                                                        ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                             >  SRR19973069.3209644/1‑150 (MQ=60)
                                                                        ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                             >  SRR19973069.934310/1‑150 (MQ=60)
                                                                        ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                             >  SRR19973069.3777036/1‑150 (MQ=60)
                                                                        ATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCA                                                                             >  SRR19973069.3777047/1‑150 (MQ=60)
                                                                           CCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCG                                                                          <  SRR19973069.239120/150‑1 (MQ=60)
                                                                            CGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGG                                                                         <  SRR19973069.3822072/150‑1 (MQ=60)
                                                                             GCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGT                                                                        <  SRR19973069.2984117/150‑1 (MQ=60)
                                                                             GCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGT                                                                        <  SRR19973069.3728038/150‑1 (MQ=60)
                                                                                CAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATC                                                                     <  SRR19973069.945959/150‑1 (MQ=60)
                                                                                CAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATC                                                                     <  SRR19973069.1586693/150‑1 (MQ=60)
                                                                                 AGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCT                                                                    <  SRR19973069.1315678/150‑1 (MQ=60)
                                                                                 AGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCT                                                                    <  SRR19973069.314226/150‑1 (MQ=60)
                                                                                  GCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTT                                                                   <  SRR19973069.2519608/150‑1 (MQ=60)
                                                                                      GGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAGAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGG                                                               <  SRR19973069.3277249/150‑1 (MQ=60)
                                                                                         GGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGT                                                            >  SRR19973069.1107946/1‑150 (MQ=60)
                                                                                         GGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGT                                                            >  SRR19973069.143168/1‑150 (MQ=60)
                                                                                          GCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTT                                                           <  SRR19973069.2617964/150‑1 (MQ=60)
                                                                                               GGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAG                                                      >  SRR19973069.493133/1‑150 (MQ=60)
                                                                                                     CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                >  SRR19973069.2211520/1‑150 (MQ=60)
                                                                                                     CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                >  SRR19973069.2392432/1‑150 (MQ=60)
                                                                                                     CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                >  SRR19973069.1772936/1‑150 (MQ=60)
                                                                                                     CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                >  SRR19973069.3464658/1‑150 (MQ=60)
                                                                                                     CGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTT                                                >  SRR19973069.2426975/1‑150 (MQ=60)
                                                                                                      GCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTT                                               <  SRR19973069.2684730/150‑1 (MQ=60)
                                                                                                      GCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTT                                               <  SRR19973069.2685196/150‑1 (MQ=60)
                                                                                                      GCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTT                                               <  SRR19973069.2685213/150‑1 (MQ=60)
                                                                                                       CTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTG                                              >  SRR19973069.740600/1‑150 (MQ=60)
                                                                                                       CTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTG                                              >  SRR19973069.1269605/1‑150 (MQ=60)
                                                                                                       CTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTG                                              >  SRR19973069.1728253/1‑150 (MQ=60)
                                                                                                       CTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGAACATCAGCGGTATCTTCTGGGGTTACCAGAGCGTTTG                                              >  SRR19973069.3324117/1‑150 (MQ=60)
                                                                                                          CGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCG                                           >  SRR19973069.241191/1‑150 (MQ=60)
                                                                                                          CGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCG                                           >  SRR19973069.241203/1‑150 (MQ=60)
                                                                                                             TAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTA                                        >  SRR19973069.3203679/1‑150 (MQ=60)
                                                                                                                ACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGG                                     >  SRR19973069.3093643/1‑150 (MQ=60)
                                                                                                                ACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGG                                     >  SRR19973069.1176562/1‑150 (MQ=60)
                                                                                                                ACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGG                                     >  SRR19973069.2999465/1‑150 (MQ=60)
                                                                                                                  ATAAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAG                                   <  SRR19973069.488962/150‑1 (MQ=60)
                                                                                                                    AAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGT                                 >  SRR19973069.931291/1‑150 (MQ=60)
                                                                                                                    AAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGT                                 <  SRR19973069.2686059/150‑1 (MQ=60)
                                                                                                                    AAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGT                                 >  SRR19973069.1327676/1‑150 (MQ=60)
                                                                                                                    AAATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGT                                 >  SRR19973069.932227/1‑150 (MQ=60)
                                                                                                                      ATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCA                               >  SRR19973069.3012686/1‑150 (MQ=60)
                                                                                                                      ATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCA                               >  SRR19973069.911711/1‑150 (MQ=60)
                                                                                                                      ATACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCA                               >  SRR19973069.2837245/1‑150 (MQ=60)
                                                                                                                       TACGTAGGCTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGTCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCAT                              >  SRR19973069.2647100/1‑150 (MQ=60)
                                                                                                                               CTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAT                      <  SRR19973069.1851811/150‑1 (MQ=60)
                                                                                                                               CTGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAT                      <  SRR19973069.1853307/150‑1 (MQ=60)
                                                                                                                                TGGCGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATT                     >  SRR19973069.2523955/1‑150 (MQ=60)
                                                                                                                                   CGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAC                  <  SRR19973069.829655/150‑1 (MQ=60)
                                                                                                                                   CGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAC                  <  SRR19973069.2941729/150‑1 (MQ=60)
                                                                                                                                   CGAAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAC                  >  SRR19973069.1509583/1‑150 (MQ=60)
                                                                                                                                     AAGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGACATTAACCA                >  SRR19973069.570283/1‑150 (MQ=60)
                                                                                                                                      AGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAG               <  SRR19973069.300915/150‑1 (MQ=60)
                                                                                                                                      AGAACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAG               <  SRR19973069.301124/150‑1 (MQ=60)
                                                                                                                                        AACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTG             >  SRR19973069.1280315/1‑150 (MQ=60)
                                                                                                                                         ACCGCTCACACCTACCTCCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGC            <  SRR19973069.1418716/150‑1 (MQ=60)
                                                                                                                                         ACCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGC            <  SRR19973069.1419021/150‑1 (MQ=60)
                                                                                                                                          CCGCTCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCG           <  SRR19973069.3833469/150‑1 (MQ=60)
                                                                                                                                              TCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG       >  SRR19973069.2716916/1‑150 (MQ=60)
                                                                                                                                              TCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG       >  SRR19973069.787898/1‑150 (MQ=60)
                                                                                                                                              TCACACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAG       <  SRR19973069.3662061/150‑1 (MQ=60)
                                                                                                                                                  ACCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTT   >  SRR19973069.2483478/1‑150 (MQ=60)
                                                                                                                                                   CCTACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTC  <  SRR19973069.3623523/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
CCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTC  >  CP009273/400445‑400741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑