Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,122,266 G→T 100% S35* (TCG→TAG)  wcaF ← putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,122,2660GT99.2% 823.2 / ‑2.1 254S35* (TCG→TAG) wcaFputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (150/101);  total (151/103)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

TCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCG  >  CP009273/2122122‑2122410
                                                                                                                                                |                                                                                                                                                
tCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTAcc                                                                                                                                               <  1:354143/148‑1 (MQ=255)
 cATCGCCGACCCACGCGTAAACACCTAAGGTTAATTTCCACGTATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCa                                                                                                                                              <  2:2218297/148‑1 (MQ=255)
      ccGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCaa                                                                                                                                         >  1:935783/1‑148 (MQ=255)
         aCCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAata                                                                                                                                      >  2:3507118/1‑148 (MQ=255)
              cgcgTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATAttgtt                                                                                                                                 >  1:1363736/1‑148 (MQ=255)
              cgcgTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATAttgtt                                                                                                                                 >  2:1408547/1‑148 (MQ=255)
                  tAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCt                                                                                                                             >  1:1748507/1‑148 (MQ=255)
                   aaTCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACTGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTg                                                                                                                            >  1:2500746/1‑148 (MQ=255)
                    aTCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGt                                                                                                                           >  1:1952570/1‑148 (MQ=255)
                     tCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTa                                                                                                                          >  1:2904397/1‑148 (MQ=255)
                     tCACCTAAGGTTAATGTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTa                                                                                                                          <  2:1867313/148‑1 (MQ=255)
                      cACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTAc                                                                                                                         >  1:2175608/1‑148 (MQ=255)
                      cACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTAc                                                                                                                         >  2:1742795/1‑148 (MQ=255)
                         cTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGc                                                                                                                      >  1:576166/1‑148 (MQ=255)
                         cTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGc                                                                                                                      >  2:2509261/1‑148 (MQ=255)
                          tAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGcc                                                                                                                     >  2:430567/1‑148 (MQ=255)
                              gTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCccacc                                                                                                                 <  1:2529051/148‑1 (MQ=255)
                              gTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCccacc                                                                                                                 >  1:2812098/1‑148 (MQ=255)
                              gTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCccacc                                                                                                                 >  2:1149184/1‑148 (MQ=255)
                                  aTTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATaa                                                                                                             <  2:3146491/148‑1 (MQ=255)
                                   tttCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAAt                                                                                                            >  2:2759720/1‑148 (MQ=255)
                                   tttCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAAt                                                                                                            >  2:3316817/1‑148 (MQ=255)
                                    ttCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAAtt                                                                                                           >  1:1098717/1‑148 (MQ=255)
                                    ttCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAAtt                                                                                                           >  1:1916006/1‑148 (MQ=255)
                                           gATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACttt                                                                                                    <  1:2621042/148‑1 (MQ=255)
                                           gATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACttt                                                                                                    <  1:52020/148‑1 (MQ=255)
                                            aTAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTa                                                                                                   >  1:363435/1‑148 (MQ=255)
                                              aGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAAt                                                                                                 >  2:2057954/1‑148 (MQ=255)
                                                gTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAg                                                                                               >  1:2370765/1‑148 (MQ=255)
                                                gTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAg                                                                                               <  1:2759720/148‑1 (MQ=255)
                                                 tAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGc                                                                                              >  1:864420/1‑148 (MQ=255)
                                                 tAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGc                                                                                              >  2:734136/1‑148 (MQ=255)
                                                  aaTTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCg                                                                                             <  1:939916/148‑1 (MQ=255)
                                                   aTTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGt                                                                                            >  1:1573149/1‑148 (MQ=255)
                                                    tttttACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGtt                                                                                           <  2:2675111/148‑1 (MQ=255)
                                                           tGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGccc                                                                                    <  1:3014113/148‑1 (MQ=255)
                                                                 acgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAAc                                                                              >  2:1390588/1‑148 (MQ=255)
                                                                  cgAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAAcc                                                                             <  2:481463/148‑1 (MQ=255)
                                                                    aaTAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCt                                                                           <  1:2022305/148‑1 (MQ=255)
                                                                     aTAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCtt                                                                          <  2:1251715/148‑1 (MQ=255)
                                                                     aTAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCtt                                                                          <  2:1361777/148‑1 (MQ=255)
                                                                     aTAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCtt                                                                          <  1:2717186/148‑1 (MQ=255)
                                                                     aTAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCtt                                                                          <  2:3511540/148‑1 (MQ=255)
                                                                     aTAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCtt                                                                          <  2:3288119/148‑1 (MQ=255)
                                                                       aaCTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTATTAGCGTTGCCGCCCCGGAACCCTTTc                                                                        >  1:320330/1‑148 (MQ=255)
                                                                       aaCTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTc                                                                        >  2:2093188/1‑148 (MQ=255)
                                                                       aaCTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTc                                                                        <  2:2703666/148‑1 (MQ=255)
                                                                       aaCTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTc                                                                        >  1:321840/1‑148 (MQ=255)
                                                                         cTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAg                                                                                               >  1:1719524/1‑123 (MQ=255)
                                                                         cTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCgg                                                                      >  1:2196653/1‑148 (MQ=255)
                                                                                   tCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCACATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGaaa                                                            >  2:1567511/1‑148 (MQ=255)
                                                                                   tCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGaaa                                                            >  1:441981/1‑148 (MQ=255)
                                                                                    ccTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAAc                                                           >  2:2651749/1‑148 (MQ=255)
                                                                                     cTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAAcc                                                          >  2:920512/1‑148 (MQ=255)
                                                                                      tATTTTTGCTCCGAATATACGTAATAAAAAAGCCCGCCAGCGATACTATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCg                                                         <  2:764066/148‑1 (MQ=255)
                                                                                      tATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCg                                                         >  2:2326409/1‑148 (MQ=255)
                                                                                        tttttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCt                                                       >  2:1123283/1‑148 (MQ=255)
                                                                                        tttttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCt                                                       >  1:1628708/1‑148 (MQ=255)
                                                                                        tttttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCt                                                       >  2:2370595/1‑148 (MQ=255)
                                                                                        tttttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCt                                                       <  1:1053462/148‑1 (MQ=255)
                                                                                         ttttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCtt                                                      >  1:434995/1‑148 (MQ=255)
                                                                                           ttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTaa                                                    >  2:3169469/1‑148 (MQ=255)
                                                                                           ttGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTaa                                                    >  1:1322801/1‑148 (MQ=255)
                                                                                            tGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTaaa                                                   <  2:2812098/148‑1 (MQ=255)
                                                                                             gCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAAt                                                  <  2:2571424/148‑1 (MQ=255)
                                                                                             gCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAAt                                                  <  2:1196469/148‑1 (MQ=255)
                                                                                              cTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATc                                                 <  1:2999043/148‑1 (MQ=255)
                                                                                               tCCGAATAAACGGAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCACATATTGTTGCCTGTACTGCCCACCATAATGGCACTCTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCt                                                <  2:1691965/148‑1 (MQ=255)
                                                                                                   aaTAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCa                                            <  1:1217440/148‑1 (MQ=255)
                                                                                                   aaTAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCa                                            <  2:1798732/148‑1 (MQ=255)
                                                                                                       aaCGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTgg                                        >  1:2095181/1‑148 (MQ=255)
                                                                                                        aCGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGt                                       <  1:2363001/148‑1 (MQ=255)
                                                                                                         cGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGtt                                      >  2:1992214/1‑148 (MQ=255)
                                                                                                         cGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGtt                                      >  2:1991525/1‑148 (MQ=255)
                                                                                                            aataaAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTc                                   >  1:217837/1‑148 (MQ=255)
                                                                                                            aataaAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTc                                   >  1:733608/1‑148 (MQ=255)
                                                                                                            aataaAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTc                                   >  1:733653/1‑148 (MQ=255)
                                                                                                               aaaaaaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCtt                                <  2:1098717/148‑1 (MQ=255)
                                                                                                               aaaaaaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCtt                                >  1:920457/1‑148 (MQ=255)
                                                                                                               aaaaaaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCtt                                >  1:2674622/1‑148 (MQ=255)
                                                                                                                 aaaaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTtat                              <  1:1408547/148‑1 (MQ=255)
                                                                                                                 aaaaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTtat                              <  1:3388018/148‑1 (MQ=255)
                                                                                                                  aaaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTtata                             <  1:1277585/148‑1 (MQ=255)
                                                                                                                   aaGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTatat                            <  2:916455/148‑1 (MQ=255)
                                                                                                                    aGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt                           <  2:1432315/148‑1 (MQ=255)
                                                                                                                     gCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTc                          <  2:1630135/148‑1 (MQ=255)
                                                                                                                            aGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACttt                   >  1:1597578/1‑148 (MQ=255)
                                                                                                                             gCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTg                  >  2:2161086/1‑148 (MQ=255)
                                                                                                                             gCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTg                  >  2:536610/1‑148 (MQ=255)
                                                                                                                              cGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAg                        <  2:3414529/141‑1 (MQ=255)
                                                                                                                                aTACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTta               <  1:2264209/148‑1 (MQ=255)
                                                                                                                                 tACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTtat              >  1:828257/1‑148 (MQ=255)
                                                                                                                                   cAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTtatat            >  2:1221110/1‑148 (MQ=255)
                                                                                                                                     aTACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATaa          >  1:1741968/1‑148 (MQ=255)
                                                                                                                                      tACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAg         >  1:2882473/1‑148 (MQ=255)
                                                                                                                                        cTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGc       >  2:157408/1‑148 (MQ=255)
                                                                                                                                        cTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGc       <  2:935783/148‑1 (MQ=255)
                                                                                                                                         ttGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCt      <  2:411808/148‑1 (MQ=255)
                                                                                                                                          tgtgGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCtt     <  2:363435/148‑1 (MQ=255)
                                                                                                                                             ggCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCg  >  2:2648422/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCG  >  CP009273/2122122‑2122410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCT  >  CP009273/2122118‑2122413
                                                                                                                                                    |                                                                                                                                                   
GACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTA                                                                                                                                                    <  SRR19973065.399696/150‑1 (MQ=60)
GACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTA                                                                                                                                                    <  SRR19973065.399699/150‑1 (MQ=60)
    TCATCGCCGACCCCCGCTTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAG                                                                                                                                                <  SRR19973065.356699/150‑1 (MQ=60)
    TCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAG                                                                                                                                                <  SRR19973065.356635/150‑1 (MQ=60)
     CATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGG                                                                                                                                               >  SRR19973065.1370792/1‑150 (MQ=60)
        CGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAA                                                                                                                                            >  SRR19973065.941986/1‑150 (MQ=60)
        CGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAA                                                                                                                                            >  SRR19973065.941966/1‑150 (MQ=60)
        CGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAA                                                                                                                                            >  SRR19973065.941955/1‑150 (MQ=60)
             ACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATT                                                                                                                                       <  SRR19973065.2765984/150‑1 (MQ=60)
              CCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTG                                                                                                                                      >  SRR19973065.907768/1‑150 (MQ=60)
              CCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTG                                                                                                                                      <  SRR19973065.532359/150‑1 (MQ=60)
                CACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTT                                                                                                                                    >  SRR19973065.1372758/1‑150 (MQ=60)
                 ACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTG                                                                                                                                   <  SRR19973065.2200441/150‑1 (MQ=60)
                 ACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTG                                                                                                                                   <  SRR19973065.2200533/150‑1 (MQ=60)
                    CGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCT                                                                                                                                >  SRR19973065.1760025/1‑150 (MQ=60)
                    CGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCT                                                                                                                                >  SRR19973065.1759937/1‑150 (MQ=60)
                     GTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACTGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTG                                                                                                                               >  SRR19973065.2516470/1‑150 (MQ=60)
                     GTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACTGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTG                                                                                                                               >  SRR19973065.1608911/1‑150 (MQ=60)
                      TAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGT                                                                                                                              >  SRR19973065.1965329/1‑150 (MQ=60)
                       AATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCCAATATTGTTGCCTGTA                                                                                                                             >  SRR19973065.2922097/1‑150 (MQ=60)
                       AATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTA                                                                                                                             >  SRR19973065.2922105/1‑150 (MQ=60)
                        ATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTAC                                                                                                                            >  SRR19973065.491865/1‑150 (MQ=60)
                        ATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTAC                                                                                                                            >  SRR19973065.2189561/1‑150 (MQ=60)
                        ATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTAC                                                                                                                            >  SRR19973065.2189562/1‑150 (MQ=60)
                           ACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGC                                                                                                                         >  SRR19973065.2353814/1‑150 (MQ=60)
                           ACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGC                                                                                                                         >  SRR19973065.579781/1‑150 (MQ=60)
                                AGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACC                                                                                                                    >  SRR19973065.2829485/1‑150 (MQ=60)
                                AGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACC                                                                                                                    >  SRR19973065.2829479/1‑150 (MQ=60)
                                 GGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCA                                                                                                                   >  SRR19973065.1517155/1‑150 (MQ=60)
                                  GTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCAT                                                                                                                  <  SRR19973065.2544925/150‑1 (MQ=60)
                                      ATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATT                                                                                                              >  SRR19973065.2990390/1‑150 (MQ=60)
                                      ATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATT                                                                                                              >  SRR19973065.1105944/1‑150 (MQ=60)
                                      ATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATT                                                                                                              >  SRR19973065.2532768/1‑150 (MQ=60)
                                      ATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATT                                                                                                              >  SRR19973065.1928577/1‑150 (MQ=60)
                                      ATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATT                                                                                                              >  SRR19973065.2862692/1‑150 (MQ=60)
                                      ATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATT                                                                                                              >  SRR19973065.2862736/1‑150 (MQ=60)
                                         TCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCA                                                                                                           <  SRR19973065.1915441/150‑1 (MQ=60)
                                          CCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCAC                                                                                                          >  SRR19973065.1737936/1‑150 (MQ=60)
                                           CACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACT                                                                                                         <  SRR19973065.2434270/150‑1 (MQ=60)
                                              GGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTA                                                                                                      >  SRR19973065.365966/1‑150 (MQ=60)
                                              GGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTA                                                                                                      >  SRR19973065.365978/1‑150 (MQ=60)
                                               GATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAA                                                                                                     <  SRR19973065.2637265/150‑1 (MQ=60)
                                               GATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAA                                                                                                     <  SRR19973065.52590/150‑1 (MQ=60)
                                                 TAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATA                                                                                                   >  SRR19973065.1970360/1‑150 (MQ=60)
                                                  AGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAG                                                                                                  >  SRR19973065.2385551/1‑150 (MQ=60)
                                                   GGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGC                                                                                                 >  SRR19973065.238272/1‑150 (MQ=60)
                                                   GGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGC                                                                                                 >  SRR19973065.2639425/1‑150 (MQ=60)
                                                   GGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGC                                                                                                 >  SRR19973065.2072640/1‑150 (MQ=60)
                                                   GGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGC                                                                                                 >  SRR19973065.869979/1‑150 (MQ=60)
                                                    GTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCG                                                                                                <  SRR19973065.3337047/150‑1 (MQ=60)
                                                    GTAATTTTTACTGACGGAAGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCG                                                                                                <  SRR19973065.2776878/150‑1 (MQ=60)
                                                     TAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGT                                                                                               >  SRR19973065.1583620/1‑150 (MQ=60)
                                                      AATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTT                                                                                              <  SRR19973065.793621/150‑1 (MQ=60)
                                                      AATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTT                                                                                              <  SRR19973065.946124/150‑1 (MQ=60)
                                                      AATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTT                                                                                              <  SRR19973065.223726/150‑1 (MQ=60)
                                                            TACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCC                                                                                        >  SRR19973065.2768984/1‑150 (MQ=60)
                                                               TGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCG                                                                                     <  SRR19973065.3032570/150‑1 (MQ=60)
                                                               TGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCG                                                                                     <  SRR19973065.3032574/150‑1 (MQ=60)
                                                               TGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCG                                                                                     <  SRR19973065.1274581/150‑1 (MQ=60)
                                                               TGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCG                                                                                     <  SRR19973065.3032565/150‑1 (MQ=60)
                                                               TGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCG                                                                                     >  SRR19973065.414689/1‑150 (MQ=60)
                                                                  CGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAA                                                                                  >  SRR19973065.2637862/1‑150 (MQ=60)
                                                                    GACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACC                                                                                <  SRR19973065.1950347/150‑1 (MQ=60)
                                                                        AATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTT                                                                            <  SRR19973065.2035313/150‑1 (MQ=60)
                                                                         ATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTATTAGCGTTGCCGCCCCGGAACCCTTTC                                                                           >  SRR19973065.322538/1‑150 (MQ=60)
                                                                         ATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTC                                                                           <  SRR19973065.2732781/150‑1 (MQ=60)
                                                                         ATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTC                                                                           <  SRR19973065.2734088/150‑1 (MQ=60)
                                                                         ATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTC                                                                           >  SRR19973065.324067/1‑150 (MQ=60)
                                                                         ATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTC                                                                           >  SRR19973065.322858/1‑150 (MQ=60)
                                                                           AACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGagatcggaagagcacacgtctga                                                                         >  SRR19973065.1730949/1‑127 (MQ=60)
                                                                           AACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGG                                                                         >  SRR19973065.2210726/1‑150 (MQ=60)
                                                                                CGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCG                                                                    >  SRR19973065.915099/1‑150 (MQ=60)
                                                                                   TTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGA                                                                 >  SRR19973065.1666671/1‑150 (MQ=60)
                                                                                    TTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAA                                                                >  SRR19973065.1080505/1‑150 (MQ=60)
                                                                                    TTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAA                                                                >  SRR19973065.1389081/1‑150 (MQ=60)
                                                                                     TTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAA                                                               >  SRR19973065.445042/1‑150 (MQ=60)
                                                                                      TTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAAC                                                              >  SRR19973065.1289629/1‑150 (MQ=60)
                                                                                      TTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAAC                                                              >  SRR19973065.2109849/1‑150 (MQ=60)
                                                                                          TATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCT                                                          >  SRR19973065.1639626/1‑150 (MQ=60)
                                                                                          TATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCT                                                          >  SRR19973065.3389065/1‑150 (MQ=60)
                                                                                           ATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTT                                                         >  SRR19973065.438020/1‑150 (MQ=60)
                                                                                            TTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTA                                                        <  SRR19973065.1060446/150‑1 (MQ=60)
                                                                                             TTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAA                                                       <  SRR19973065.1965686/150‑1 (MQ=60)
                                                                                             TTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAA                                                       >  SRR19973065.1331548/1‑150 (MQ=60)
                                                                                                 GCTCCGAATAAACTTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGAACTGACCACCATAATTGCACTTTAATATCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCT                                                   >  SRR19973065.2417331/1‑150 (MQ=60)
                                                                                                  CTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTT                                                  <  SRR19973065.3017417/150‑1 (MQ=60)
                                                                                                  CTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTT                                                  >  SRR19973065.625543/1‑150 (MQ=60)
                                                                                                  CTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTT                                                  >  SRR19973065.625669/1‑150 (MQ=60)
                                                                                                   TCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTG                                                 <  SRR19973065.84532/150‑1 (MQ=60)
                                                                                                     CGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCA                                               >  SRR19973065.484522/1‑150 (MQ=60)
                                                                                                       AATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATT                                             <  SRR19973065.1225343/150‑1 (MQ=60)
                                                                                                         TAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGACCCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGG                                           >  SRR19973065.1592494/1‑150 (MQ=60)
                                                                                                         TAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGG                                           >  SRR19973065.432610/1‑150 (MQ=60)
                                                                                                         TAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGG                                           >  SRR19973065.2108647/1‑150 (MQ=60)
                                                                                                         TAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGG                                           >  SRR19973065.2109324/1‑150 (MQ=60)
                                                                                                            ACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTT                                        <  SRR19973065.2377708/150‑1 (MQ=60)
                                                                                                              GTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTC                                      >  SRR19973065.738514/1‑150 (MQ=60)
                                                                                                              GTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTC                                      >  SRR19973065.738538/1‑150 (MQ=60)
                                                                                                              GTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTC                                      >  SRR19973065.219466/1‑150 (MQ=60)
                                                                                                              GTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTC                                      >  SRR19973065.738559/1‑150 (MQ=60)
                                                                                                                 ATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTT                                   >  SRR19973065.2691200/1‑150 (MQ=60)
                                                                                                                 ATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTT                                   >  SRR19973065.926489/1‑150 (MQ=60)
                                                                                                                    AAAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATA                                <  SRR19973065.1754296/150‑1 (MQ=60)
                                                                                                                     AAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAT                               <  SRR19973065.2331324/150‑1 (MQ=60)
                                                                                                                     AAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAT                               <  SRR19973065.1417756/150‑1 (MQ=60)
                                                                                                                     AAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAT                               <  SRR19973065.1417763/150‑1 (MQ=60)
                                                                                                                     AAAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAT                               <  SRR19973065.3408738/150‑1 (MQ=60)
                                                                                                                      AAAGCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATT                              <  SRR19973065.1286019/150‑1 (MQ=60)
                                                                                                                        AGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGAACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCA                            >  SRR19973065.1783461/1‑150 (MQ=60)
                                                                                                                         GCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAG                           <  SRR19973065.1694399/150‑1 (MQ=60)
                                                                                                                         GCCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAAGAGCGTTGCCGCCCCGGAACCCTTTAGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAG                           <  SRR19973065.3180222/150‑1 (MQ=60)
                                                                                                                          CCCGCCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGA                          >  SRR19973065.1325104/1‑150 (MQ=60)
                                                                                                                              CCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTT                      >  SRR19973065.1608216/1‑150 (MQ=60)
                                                                                                                              CCAGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTT                      >  SRR19973065.1608248/1‑150 (MQ=60)
                                                                                                                                AGCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACAGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACagatc                    >  SRR19973065.3435393/1‑145 (MQ=60)
                                                                                                                                 GCGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTT                   <  SRR19973065.943232/150‑1 (MQ=60)
                                                                                                                                  CGATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTA                  >  SRR19973065.1154219/1‑150 (MQ=60)
                                                                                                                                   GATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTAT                 >  SRR19973065.832488/1‑150 (MQ=60)
                                                                                                                                   GATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTAT                 >  SRR19973065.833676/1‑150 (MQ=60)
                                                                                                                                    ATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATA                <  SRR19973065.2616699/150‑1 (MQ=60)
                                                                                                                                    ATACAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATA                <  SRR19973065.2278523/150‑1 (MQ=60)
                                                                                                                                       CAATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAA             >  SRR19973065.1753466/1‑150 (MQ=60)
                                                                                                                                        AATACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAG            >  SRR19973065.2900112/1‑150 (MQ=60)
                                                                                                                                          TACTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGC          <  SRR19973065.1209781/150‑1 (MQ=60)
                                                                                                                                            CTTGTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTT        >  SRR19973065.259870/1‑150 (MQ=60)
                                                                                                                                               GTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCG     <  SRR19973065.2963954/150‑1 (MQ=60)
                                                                                                                                               GTGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCG     <  SRR19973065.440217/150‑1 (MQ=60)
                                                                                                                                                TGGCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGT    >  SRR19973065.3438325/1‑150 (MQ=60)
                                                                                                                                                  GCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCT  <  SRR19973065.1399890/150‑1 (MQ=60)
                                                                                                                                                  GCTACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCT  <  SRR19973065.1399720/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
GACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTGCTCCGAATAAACGTAATAAAAAAGCCCGCCAGCGATACAATACTTGTGGCGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCT  >  CP009273/2122118‑2122413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑