Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,279,816 G→T 100% A27A (GCG→GCT)  nrfC → formate‑dependent nitrite reductase, 4Fe4S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,279,8160GT99.5% 644.7 / ‑4.3 200A27A (GCG→GCT) nrfCformate‑dependent nitrite reductase, 4Fe4S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (86/113);  total (86/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

CACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAG  >  CP009273/4279671‑4279960
                                                                                                                                                 |                                                                                                                                                
  caGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTaaaa                                                                                                                                              >  2:1373221/1‑148 (MQ=255)
     cGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGt                                                                                                                                           <  2:2378622/148‑1 (MQ=255)
      gATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGtt                                                                                                                                          <  2:528237/148‑1 (MQ=255)
       aTCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTg                                                                                                                                         <  1:951518/148‑1 (MQ=255)
        tCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGa                                                                                                                                        <  2:1346348/148‑1 (MQ=255)
         cAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGaa                                                                                                                                       >  1:3339233/1‑148 (MQ=255)
         atgcgcACGAATGCGAACTTTAACTCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCAGCGGCGTCGGCGTGGTGGCTGCCGTCAGCGGCTCCGCAGGGCGCGTGGTGGCTAAATGGTTGaa                                                                                                                                       <  2:2021803/146‑1 (MQ=255)
          aGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAat                                                                                                                                      <  2:1008294/148‑1 (MQ=255)
          aGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAat                                                                                                                                      <  1:2302021/148‑1 (MQ=255)
            cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGaggtg                                                                                                                                    >  2:3207673/1‑144 (MQ=255)
            cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAatat                                                                                                                                    >  2:3252935/1‑148 (MQ=255)
              cACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCa                                                                                                                                  <  1:3081110/148‑1 (MQ=255)
               aCCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCaa                                                                                                                                 <  1:794737/148‑1 (MQ=255)
               aCCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCaa                                                                                                                                 <  1:1358609/148‑1 (MQ=255)
                 cAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATg                                                                                                                               >  2:925547/1‑148 (MQ=255)
                  aaTCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATgg                                                                                                                              <  2:449010/148‑1 (MQ=255)
                   aTCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCANCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATggg                                                                                                                             <  1:1783921/148‑1 (MQ=255)
                    tCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTCAAACGTTGAATATCAATgggg                                                                                                                            >  2:2997802/1‑148 (MQ=255)
                    tCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATgggg                                                                                                                            >  2:3158605/1‑148 (MQ=255)
                         aCTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGt                                                                                                                       >  1:1214764/1‑148 (MQ=255)
                         aCTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGt                                                                                                                       >  1:1215104/1‑148 (MQ=255)
                           tttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTa                                                                                                                     >  2:3343255/1‑148 (MQ=255)
                                cccGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCa                                                                                                                <  2:1506448/148‑1 (MQ=255)
                                  cGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATg                                                                                                              <  1:1615627/148‑1 (MQ=255)
                                   ggCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATgg                                                                                                             <  2:1909255/148‑1 (MQ=255)
                                   ggCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATgg                                                                                                             <  2:964357/148‑1 (MQ=255)
                                   ggCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATgg                                                                                                             <  2:2842609/148‑1 (MQ=255)
                                     cGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTg                                                                                                           <  1:2111715/148‑1 (MQ=255)
                                       tCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAACACGTTGAAGATCAATGGGGTGCGTTACGGCATGGTGCa                                                                                                         <  2:2297956/148‑1 (MQ=255)
                                       tCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCa                                                                                                         <  2:2298033/148‑1 (MQ=255)
                                         tGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATg                                                                                                       >  1:267998/1‑148 (MQ=255)
                                         tGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATg                                                                                                       >  2:1905840/1‑148 (MQ=255)
                                          gTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGa                                                                                                      <  2:1275897/148‑1 (MQ=255)
                                           ttCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGAc                                                                                                     >  1:1526991/1‑148 (MQ=255)
                                            tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACg                                                                                                    >  2:1075832/1‑148 (MQ=255)
                                            tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACg                                                                                                    >  2:1075670/1‑148 (MQ=255)
                                             ccGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGa                                                                                                   <  1:2527846/148‑1 (MQ=255)
                                             ccGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGa                                                                                                   <  2:2261845/148‑1 (MQ=255)
                                             ccGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGa                                                                                                   <  2:1612706/148‑1 (MQ=255)
                                               gTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGt                                                                                                 >  1:762320/1‑148 (MQ=255)
                                                   cTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGtt                                                                                             <  2:584961/148‑1 (MQ=255)
                                                   cTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGtt                                                                                             >  1:1632861/1‑148 (MQ=255)
                                                    ttAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGTTGCGTTACGGCATGGTGCATGACGAGTCGTTa                                                                                            <  2:3236659/148‑1 (MQ=255)
                                                    ttAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTa                                                                                            <  1:249397/148‑1 (MQ=255)
                                                     tAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTAt                                                                                           >  2:2945337/1‑148 (MQ=255)
                                                       aGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGc                                                                                         >  1:546871/1‑148 (MQ=255)
                                                        ggAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCa                                                                                        >  1:1160140/1‑148 (MQ=255)
                                                        ggAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCa                                                                                        >  2:3137924/1‑148 (MQ=255)
                                                        ggAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCa                                                                                        >  1:3489436/1‑148 (MQ=255)
                                                         gAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCAt                                                                                       >  1:284342/1‑148 (MQ=255)
                                                         gAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCAt                                                                                       <  2:3303886/148‑1 (MQ=255)
                                                          agcagcCATGACCTGGTCTCGTCGCCAGTTTCTCACAGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATc                                                                                      <  1:952346/148‑1 (MQ=255)
                                                          agcagcCATGACCTGGTCTCGTCGCCAGTTTCTCACAGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATc                                                                                      <  1:952387/148‑1 (MQ=255)
                                                               ccATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTg                                                                                 <  2:2236681/148‑1 (MQ=255)
                                                                cATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGt                                                                                <  1:2410095/148‑1 (MQ=255)
                                                                 aTGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTTGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTa                                                                               <  1:1480996/148‑1 (MQ=255)
                                                                 aTGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTa                                                                               <  1:1929344/148‑1 (MQ=255)
                                                                 aTGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTa                                                                               <  1:2530456/148‑1 (MQ=255)
                                                                     ccTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGc                                                                           <  1:157001/148‑1 (MQ=255)
                                                                      cTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGcc                                                                          <  1:2570525/148‑1 (MQ=255)
                                                                       tGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCt                                                                         >  1:2799817/1‑148 (MQ=255)
                                                                       tGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCt                                                                         >  1:1048609/1‑148 (MQ=255)
                                                                         gTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGt                                                                       <  2:790177/148‑1 (MQ=255)
                                                                         gTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGt                                                                       <  1:454289/148‑1 (MQ=255)
                                                                           ctcGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTAt                                                                     <  1:2028845/148‑1 (MQ=255)
                                                                             cgtcgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTGGCATATGAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATgg                                                                   <  2:578731/148‑1 (MQ=255)
                                                                                cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATg                                                                <  2:342963/148‑1 (MQ=255)
                                                                                 gCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGc                                                               <  1:2402583/148‑1 (MQ=255)
                                                                                    aGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTg                                                            <  1:3058640/148‑1 (MQ=255)
                                                                                     gTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGt                                                           <  2:3419122/148‑1 (MQ=255)
                                                                                           cACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGaa                                                     >  2:1737092/1‑148 (MQ=255)
                                                                                           cACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGaa                                                     <  2:3273233/148‑1 (MQ=255)
                                                                                              cggcgtcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTg                                                  >  1:387992/1‑148 (MQ=255)
                                                                                                 cgtcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAAc                                               >  2:2586930/1‑148 (MQ=255)
                                                                                                   tcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACaa                                             <  2:1048609/148‑1 (MQ=255)
                                                                                                   tcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACaa                                             <  2:1523352/148‑1 (MQ=255)
                                                                                                    cggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGACGTGAACAAg                                            >  2:1396708/1‑148 (MQ=255)
                                                                                                      gcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGt                                          <  2:632672/148‑1 (MQ=255)
                                                                                                            tGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGa                                    <  2:2522587/148‑1 (MQ=255)
                                                                                                             ggCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGaa                                   >  2:3244857/1‑148 (MQ=255)
                                                                                                                aGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGc                                >  2:2984704/1‑148 (MQ=255)
                                                                                                                   cGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGtc                             <  2:1586249/148‑1 (MQ=255)
                                                                                                                         cGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCgcgc                       <  2:2905052/148‑1 (MQ=255)
                                                                                                                         cGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCgcgc                       >  1:3062640/1‑148 (MQ=255)
                                                                                                                         cGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCAGGAAGGCGTCTCgcgc                       >  2:220473/1‑148 (MQ=255)
                                                                                                                            cACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTg                    <  1:925547/148‑1 (MQ=255)
                                                                                                                              ccGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGAc                  >  1:963822/1‑148 (MQ=255)
                                                                                                                                 cAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGat               >  1:1615200/1‑148 (MQ=255)
                                                                                                                                   gggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatta             <  2:353525/148‑1 (MQ=255)
                                                                                                                                   gggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatta             <  2:354035/148‑1 (MQ=255)
                                                                                                                                   gggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatta             >  2:2862849/1‑148 (MQ=255)
                                                                                                                                    ggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGattat            <  2:2360658/148‑1 (MQ=255)
                                                                                                                                     gcgcGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGattatt           <  2:1208831/148‑1 (MQ=255)
                                                                                                                                        cgTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGt        >  1:3368046/1‑148 (MQ=255)
                                                                                                                                        cgTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGt        >  2:2740766/1‑148 (MQ=255)
                                                                                                                                        cgTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGt        <  1:2914648/148‑1 (MQ=255)
                                                                                                                                            gtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCg    <  1:1342911/148‑1 (MQ=255)
                                                                                                                                            gtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCg    >  1:3467182/1‑148 (MQ=255)
                                                                                                                                              ggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAg  >  2:478725/1‑148 (MQ=255)
                                                                                                                                              ggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAg  <  1:3241955/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAG  >  CP009273/4279671‑4279960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGC  >  CP009273/4279667‑4279964
                                                                                                                                                     |                                                                                                                                                    
TTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCT                                                                                                                                                      <  SRR19973065.2751184/150‑1 (MQ=60)
        GCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTT                                                                                                                                              <  SRR19973065.865826/150‑1 (MQ=60)
          GATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGA                                                                                                                                            >  SRR19973065.3226482/1‑150 (MQ=60)
           ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAA                                                                                                                                           <  SRR19973065.957806/150‑1 (MQ=60)
           ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAA                                                                                                                                           >  SRR19973065.3359628/1‑150 (MQ=60)
           ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAA                                                                                                                                           >  SRR19973065.1610134/1‑150 (MQ=60)
              AGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATAT                                                                                                                                        <  SRR19973065.2316501/150‑1 (MQ=60)
                  CACCAATCCGAACTTTAACCCGGAGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAAT                                                                                                                                    <  SRR19973065.3099927/150‑1 (MQ=60)
                   ACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATG                                                                                                                                   <  SRR19973065.1367608/150‑1 (MQ=60)
                   ACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATG                                                                                                                                   <  SRR19973065.799995/150‑1 (MQ=60)
                    CCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGG                                                                                                                                  <  SRR19973065.2092493/150‑1 (MQ=60)
                       ATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCANCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGT                                                                                                                               <  SRR19973065.1795587/150‑1 (MQ=60)
                       ATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGT                                                                                                                               <  SRR19973065.1900791/150‑1 (MQ=60)
                           GAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGT                                                                                                                           >  SRR19973065.1222976/1‑150 (MQ=60)
                           GAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGT                                                                                                                           >  SRR19973065.1222635/1‑150 (MQ=60)
                           GAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGT                                                                                                                           >  SRR19973065.1222630/1‑150 (MQ=60)
                             ACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTA                                                                                                                         <  SRR19973065.1539378/150‑1 (MQ=60)
                              CTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTAC                                                                                                                        >  SRR19973065.1757073/1‑150 (MQ=60)
                               TTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACG                                                                                                                       >  SRR19973065.2256239/1‑150 (MQ=60)
                                      CGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGT                                                                                                                <  SRR19973065.1626459/150‑1 (MQ=60)
                                      CGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGT                                                                                                                <  SRR19973065.1626499/150‑1 (MQ=60)
                                      CGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGT                                                                                                                <  SRR19973065.1626468/150‑1 (MQ=60)
                                      CGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGT                                                                                                                <  SRR19973065.119240/150‑1 (MQ=60)
                                         CGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCA                                                                                                             <  SRR19973065.2125305/150‑1 (MQ=60)
                                           TCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATG                                                                                                           >  SRR19973065.269821/1‑150 (MQ=60)
                                            CTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGA                                                                                                          >  SRR19973065.3117285/1‑150 (MQ=60)
                                            CTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGA                                                                                                          >  SRR19973065.1365478/1‑150 (MQ=60)
                                             TGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGAC                                                                                                         <  SRR19973065.334143/150‑1 (MQ=60)
                                             TGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGAC                                                                                                         >  SRR19973065.1537026/1‑150 (MQ=60)
                                               TTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGA                                                                                                       <  SRR19973065.2783675/150‑1 (MQ=60)
                                                 CCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGT                                                                                                     <  SRR19973065.2543813/150‑1 (MQ=60)
                                                 CCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGT                                                                                                     <  SRR19973065.2543713/150‑1 (MQ=60)
                                                 CCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGT                                                                                                     >  SRR19973065.2538427/1‑150 (MQ=60)
                                                 CCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGT                                                                                                     >  SRR19973065.767396/1‑150 (MQ=60)
                                                     TGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTT                                                                                                 >  SRR19973065.1643818/1‑150 (MQ=60)
                                                        TTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATG                                                                                              <  SRR19973065.2034828/150‑1 (MQ=60)
                                                        TTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATG                                                                                              <  SRR19973065.251150/150‑1 (MQ=60)
                                                         TAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGC                                                                                             >  SRR19973065.550399/1‑150 (MQ=60)
                                                          AAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCA                                                                                            >  SRR19973065.3510655/1‑150 (MQ=60)
                                                          AAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCA                                                                                            >  SRR19973065.1167568/1‑150 (MQ=60)
                                                           AGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCAT                                                                                           >  SRR19973065.286217/1‑150 (MQ=60)
                                                            GGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATC                                                                                          >  SRR19973065.282352/1‑150 (MQ=60)
                                                              AGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACAGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGG                                                                                        <  SRR19973065.958682/150‑1 (MQ=60)
                                                              AGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACAGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGG                                                                                        <  SRR19973065.958152/150‑1 (MQ=60)
                                                              AGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACAGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGG                                                                                        <  SRR19973065.958641/150‑1 (MQ=60)
                                                              AGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACAGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGG                                                                                        <  SRR19973065.958670/150‑1 (MQ=60)
                                                                  TCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGT                                                                                    >  SRR19973065.2922760/1‑150 (MQ=60)
                                                                   CCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTA                                                                                   >  SRR19973065.1119722/1‑150 (MQ=60)
                                                                    CATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTAC                                                                                  <  SRR19973065.2425270/150‑1 (MQ=60)
                                                                     ATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTTGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACC                                                                                 <  SRR19973065.1490455/150‑1 (MQ=60)
                                                                     ATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACC                                                                                 <  SRR19973065.1941985/150‑1 (MQ=60)
                                                                     ATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACC                                                                                 <  SRR19973065.2546339/150‑1 (MQ=60)
                                                                         CCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTCAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCT                                                                             >  SRR19973065.2817161/1‑150 (MQ=60)
                                                                         CCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCT                                                                             >  SRR19973065.1055548/1‑150 (MQ=60)
                                                                         CCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCT                                                                             <  SRR19973065.158302/150‑1 (MQ=60)
                                                                          CTGGTCTCGTCGCCATTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGTTGGCTAAAACGTTGAATATCAATGGTGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTG                                                                            <  SRR19973065.342766/150‑1 (MQ=60)
                                                                          CTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTG                                                                            <  SRR19973065.2586571/150‑1 (MQ=60)
                                                                          CTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTG                                                                            <  SRR19973065.2586563/150‑1 (MQ=60)
                                                                           TGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGT                                                                           <  SRR19973065.1306497/150‑1 (MQ=60)
                                                                            GGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTA                                                                          <  SRR19973065.1436374/150‑1 (MQ=60)
                                                                             GTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTAT                                                                         <  SRR19973065.457433/150‑1 (MQ=60)
                                                                             GTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTAT                                                                         <  SRR19973065.457372/150‑1 (MQ=60)
                                                                               CTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGG                                                                       <  SRR19973065.2041874/150‑1 (MQ=60)
                                                                                     GCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTT                                                                 <  SRR19973065.2417690/150‑1 (MQ=60)
                                                                                       CAGTTTCTCCCCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGT                                                               <  SRR19973065.95516/150‑1 (MQ=60)
                                                                                        AGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTC                                                              <  SRR19973065.3081004/150‑1 (MQ=60)
                                                                                        AGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTC                                                              <  SRR19973065.3077334/150‑1 (MQ=60)
                                                                                                ACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTG                                                      >  SRR19973065.390701/1‑150 (MQ=60)
                                                                                                ACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAAGGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTG                                                      >  SRR19973065.390567/1‑150 (MQ=60)
                                                                                                   GGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCGTGTCGGGAAGTGAAC                                                   >  SRR19973065.2418666/1‑150 (MQ=60)
                                                                                                        CGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGT                                              <  SRR19973065.529719/150‑1 (MQ=60)
                                                                                                           CGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCC                                           >  SRR19973065.2439652/1‑150 (MQ=60)
                                                                                                                     GCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTC                                 >  SRR19973065.799634/1‑150 (MQ=60)
                                                                                                                      CCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCT                                <  SRR19973065.3060708/150‑1 (MQ=60)
                                                                                                                        GTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCG                              <  SRR19973065.3491314/150‑1 (MQ=60)
                                                                                                                           AGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGC                           >  SRR19973065.3081362/1‑150 (MQ=60)
                                                                                                                              GGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                        <  SRR19973065.3372355/150‑1 (MQ=60)
                                                                                                                                CACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGAC                      <  SRR19973065.931624/150‑1 (MQ=60)
                                                                                                                                CACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGAC                      >  SRR19973065.970216/1‑150 (MQ=60)
                                                                                                                                   CGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGAT                   >  SRR19973065.1626039/1‑150 (MQ=60)
                                                                                                                                     CAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTA                 >  SRR19973065.964916/1‑150 (MQ=60)
                                                                                                                                          CGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGT            >  SRR19973065.3388640/1‑150 (MQ=60)
                                                                                                                                            CGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAG          <  SRR19973065.2932376/150‑1 (MQ=60)
                                                                                                                                            CGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAG          <  SRR19973065.1532866/150‑1 (MQ=60)
                                                                                                                                             GTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC         <  SRR19973065.627632/150‑1 (MQ=60)
                                                                                                                                             GTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC         <  SRR19973065.2664018/150‑1 (MQ=60)
                                                                                                                                              TGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCG        >  SRR19973065.3488293/1‑150 (MQ=60)
                                                                                                                                                GTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAG      <  SRR19973065.1351795/150‑1 (MQ=60)
                                                                                                                                                  GGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCC    <  SRR19973065.3261497/150‑1 (MQ=60)
                                                                                                                                                  GGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCC    <  SRR19973065.2376035/150‑1 (MQ=60)
                                                                                                                                                   GCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCGGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCG   >  SRR19973065.682665/1‑150 (MQ=60)
                                                                                                                                                   GCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCGGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCG   >  SRR19973065.2453926/1‑150 (MQ=60)
                                                                                                                                                    CTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGC  >  SRR19973065.616600/1‑150 (MQ=60)
                                                                                                                                                    CTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGC  >  SRR19973065.616394/1‑150 (MQ=60)
                                                                                                                                                    CTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGGATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGC  >  SRR19973065.1497548/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGC  >  CP009273/4279667‑4279964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑