Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,279,816 G→T 55.5% A27A (GCG→GCT)  nrfC → formate‑dependent nitrite reductase, 4Fe4S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,279,8160GT55.5% 6.7 / inf 241A27A (GCG→GCT) nrfCformate‑dependent nitrite reductase, 4Fe4S subunit
Reads supporting (aligned to +/- strand):  ref base G (49/58);  new base T (69/65);  total (118/123)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

Only 100 of 241 total aligned reads displayed.

CCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC  >  CP009273/4279670‑4279957
                                                                                                                                                  |                                                                                                                                             
  acaGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTaaa                                                                                                                                            <  2:580780/148‑1 (MQ=255)
  acaGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGaaa                                                                                                                                            <  2:798227/148‑1 (MQ=255)
   caGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGaaaa                                                                                                                                           >  2:2763363/1‑148 (MQ=255)
    aGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAAc                                                                                                                                          >  2:3428664/1‑148 (MQ=255)
         tCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGa                                                                                                                                     >  1:3392622/1‑148 (MQ=255)
         tCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAAAGTTGa                                                                                                                                     >  2:2201824/1‑148 (MQ=255)
          cAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGaa                                                                                                                                    <  2:101925/148‑1 (MQ=255)
          cAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGACAACGGTGaa                                                                                                                                    >  1:9829/1‑148 (MQ=255)
          cAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGaa                                                                                                                                    >  1:1944864/1‑148 (MQ=255)
          cAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGaa                                                                                                                                    >  1:1946093/1‑148 (MQ=255)
             cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAatat                                                                                                                                 >  2:82386/1‑148 (MQ=255)
             cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAatat                                                                                                                                 >  2:1978641/1‑148 (MQ=255)
             cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAatat                                                                                                                                 <  1:2810178/148‑1 (MQ=255)
               cACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCa                                                                                                                               <  1:3466141/148‑1 (MQ=255)
               cACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCa                                                                                                                               >  2:2711611/1‑148 (MQ=255)
               cACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCa                                                                                                                               <  2:2803433/148‑1 (MQ=255)
                aCCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCaa                                                                                                                              <  1:2041822/148‑1 (MQ=255)
                  cAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATg                                                                                                                            >  2:401481/1‑148 (MQ=255)
                     tCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATgggg                                                                                                                         >  2:3787501/1‑148 (MQ=255)
                      ccGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGt                                                                                                                        >  1:280469/1‑148 (MQ=255)
                         aaCTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCTCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCg                                                                                                                     <  2:3068701/148‑1 (MQ=255)
                         aaCTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCg                                                                                                                     >  2:1392341/1‑148 (MQ=255)
                            tttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTa                                                                                                                  >  2:1300534/1‑148 (MQ=255)
                            tttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTa                                                                                                                  <  1:3355989/148‑1 (MQ=255)
                            tttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTa                                                                                                                  >  2:3205252/1‑148 (MQ=255)
                            tttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTa                                                                                                                  <  2:12825/148‑1 (MQ=255)
                             ttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTAc                                                                                                                 >  1:3016410/1‑148 (MQ=255)
                             ttAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTAc                                                                                                                 >  2:594346/1‑148 (MQ=255)
                                aCCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGc                                                                                                              <  1:3600201/148‑1 (MQ=255)
                                aCCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGc                                                                                                              >  1:457269/1‑148 (MQ=255)
                                 cccGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCa                                                                                                             <  2:1701467/148‑1 (MQ=255)
                                   cGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATg                                                                                                           <  1:401481/148‑1 (MQ=255)
                                         cTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCAt                                                                                                     >  1:1770055/1‑148 (MQ=255)
                                           gTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGa                                                                                                   <  1:347027/148‑1 (MQ=255)
                                           gTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGa                                                                                                   <  1:2492074/148‑1 (MQ=255)
                                           gTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGa                                                                                                   <  2:3664615/148‑1 (MQ=255)
                                             tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACg                                                                                                 >  2:1079091/1‑148 (MQ=255)
                                             tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACg                                                                                                 >  1:1995001/1‑148 (MQ=255)
                                             tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACg                                                                                                 >  1:1996184/1‑148 (MQ=255)
                                             tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACg                                                                                                 >  1:2160397/1‑148 (MQ=255)
                                               cggtGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAg                                                                                               <  2:1836832/145‑1 (MQ=255)
                                                gTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGt                                                                                              <  2:1709327/148‑1 (MQ=255)
                                                gTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGt                                                                                              <  2:1448514/148‑1 (MQ=255)
                                                  tGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCg                                                                                            >  1:1203775/1‑148 (MQ=255)
                                                   gCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGt                                                                                           >  1:1642529/1‑148 (MQ=255)
                                                    cTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGtt                                                                                          <  2:3592817/148‑1 (MQ=255)
                                                    cTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGtt                                                                                          <  2:2122002/148‑1 (MQ=255)
                                                     ttAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTa                                                                                         <  1:1406045/148‑1 (MQ=255)
                                                      tAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCa                                                                                                                               <  2:1586962/109‑1 (MQ=255)
                                                      tAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTAt                                                                                        >  1:1833140/1‑148 (MQ=255)
                                                        aGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGc                                                                                      <  1:1560027/148‑1 (MQ=255)
                                                        aGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGc                                                                                      <  2:1632932/148‑1 (MQ=255)
                                                         ggAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCa                                                                                     <  2:903954/148‑1 (MQ=255)
                                                         ggAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATTCa                                                                                     >  1:2831050/1‑148 (MQ=255)
                                                            gcagcCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCg                                                                                  >  2:3707239/1‑148 (MQ=255)
                                                             cagcCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCgg                                                                                 <  2:2626052/148‑1 (MQ=255)
                                                                      ccTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGc                                                                        <  2:1874574/148‑1 (MQ=255)
                                                                       cTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGcc                                                                       >  1:427912/1‑148 (MQ=255)
                                                                       cTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGcc                                                                       >  1:2349813/1‑148 (MQ=255)
                                                                         ggTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTg                                                                     >  2:157621/1‑148 (MQ=255)
                                                                         ggTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTg                                                                     >  2:313179/1‑148 (MQ=255)
                                                                         ggTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTg                                                                     >  2:3150019/1‑148 (MQ=255)
                                                                         ggTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTg                                                                     >  2:154345/1‑148 (MQ=255)
                                                                          gTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGACTATCAATGGGGTGCGTTACGGGCTGGGGCCTGAGGAGTCGTTCTGCTTCGGCTGTGCCGGCTgg                                                                    >  2:2564934/1‑147 (MQ=255)
                                                                          gTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGt                                                                    >  2:1876889/1‑148 (MQ=255)
                                                                            ctcGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTAt                                                                  >  2:3339692/1‑148 (MQ=255)
                                                                            ctcGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTAt                                                                  <  1:523230/148‑1 (MQ=255)
                                                                                 cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATg                                                             >  1:1940173/1‑148 (MQ=255)
                                                                                 cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATg                                                             <  1:3322775/148‑1 (MQ=255)
                                                                                     aGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTg                                                         >  2:3436986/1‑148 (MQ=255)
                                                                                     aGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTg                                                         <  1:102497/148‑1 (MQ=255)
                                                                                     aGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTg                                                         <  1:102689/148‑1 (MQ=255)
                                                                                            cACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGaa                                                  >  1:3312392/1‑148 (MQ=255)
                                                                                             aCCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAg                                                 >  2:948489/1‑148 (MQ=255)
                                                                                             aCCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAg                                                 >  2:2452304/1‑148 (MQ=255)
                                                                                                 gcgtcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGaa                                             <  2:2093338/148‑1 (MQ=255)
                                                                                                   gtcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACa                                           <  1:825973/148‑1 (MQ=255)
                                                                                                   gtcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACa                                           <  2:717131/148‑1 (MQ=255)
                                                                                                    tcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACaa                                          <  2:3502270/148‑1 (MQ=255)
                                                                                                         gtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGc                                     <  2:3021868/148‑1 (MQ=255)
                                                                                                         gtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCCTGGTGGCTAAAACGTTGAATATCCATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGc                                     <  2:3520799/148‑1 (MQ=255)
                                                                                                                cAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCTATGGGGTGCGTTACGGCTTGGTGCATGACGAGTCGTTATTCATCGGCTGTACCTCCTGTATGGATGCTTGTCGGGTAGTGAACAATGTGCCGGAAgg                              <  1:675952/148‑1 (MQ=255)
                                                                                                                 aGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGc                             <  2:2949264/148‑1 (MQ=255)
                                                                                                                  gCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCg                            >  2:1205145/1‑148 (MQ=255)
                                                                                                                   ccGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGt                           <  2:3385171/148‑1 (MQ=255)
                                                                                                                   ccGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGt                           >  1:666082/1‑148 (MQ=255)
                                                                                                                     gTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGtct                         <  1:594346/148‑1 (MQ=255)
                                                                                                                       cAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTcg                       >  2:307352/1‑148 (MQ=255)
                                                                                                                           ggCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGcc                   <  1:75388/148‑1 (MQ=255)
                                                                                                                           ggCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGcc                   <  1:774696/148‑1 (MQ=255)
                                                                                                                              aCCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGa                <  1:3140848/148‑1 (MQ=255)
                                                                                                                                cGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACg              >  1:2239093/1‑148 (MQ=255)
                                                                                                                                   aGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatt           <  1:3489705/148‑1 (MQ=255)
                                                                                                                                    gggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatta          <  1:1079091/148‑1 (MQ=255)
                                                                                                                                     ggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATTCTTGTCGGGAAGTGAACAAGGTGCCGGAATGCGTCTCGCGCCTGACGattat         >  1:3643029/1‑148 (MQ=255)
                                                                                                                                     ggCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGattat         >  1:263605/1‑148 (MQ=255)
                                                                                                                                        gcgTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCg      <  2:1946093/148‑1 (MQ=255)
                                                                                                                                          gtggtgGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTa    <  1:1337824/148‑1 (MQ=255)
                                                                                                                                           tggtggCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAg   <  1:2876906/148‑1 (MQ=255)
                                                                                                                                            ggtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGc  <  1:2711611/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC  >  CP009273/4279670‑4279957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCA  >  CP009273/4279668‑4279965
                                                                                                                                                    |                                                                                                                                                     
TGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGA                                                                                                                                                      >  SRR19973069.2045815/1‑150 (MQ=60)
      AGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGT                                                                                                                                                >  SRR19973069.1410729/1‑150 (MQ=60)
         GATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTCAAACGTTGA                                                                                                                                             >  SRR19973069.3414793/1‑150 (MQ=60)
         GATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGA                                                                                                                                             >  SRR19973069.3414774/1‑150 (MQ=60)
         GATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGA                                                                                                                                             >  SRR19973069.3414887/1‑150 (MQ=60)
          ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGACAACGGTGAA                                                                                                                                            >  SRR19973069.9975/1‑150 (MQ=60)
          ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAACACGTTGAA                                                                                                                                            >  SRR19973069.9989/1‑150 (MQ=60)
          ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAA                                                                                                                                            >  SRR19973069.1958279/1‑150 (MQ=60)
          ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAA                                                                                                                                            >  SRR19973069.1959514/1‑150 (MQ=60)
          ATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAA                                                                                                                                            >  SRR19973069.1959526/1‑150 (MQ=60)
           TCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAAT                                                                                                                                           <  SRR19973069.1033333/150‑1 (MQ=60)
           TCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAAT                                                                                                                                           <  SRR19973069.1076708/150‑1 (MQ=60)
               CGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCA                                                                                                                                       <  SRR19973069.2830651/150‑1 (MQ=60)
               CGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCA                                                                                                                                       <  SRR19973069.2828802/150‑1 (MQ=60)
               CGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCA                                                                                                                                       <  SRR19973069.2329077/150‑1 (MQ=60)
                 CACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAAT                                                                                                                                     <  SRR19973069.3488570/150‑1 (MQ=60)
                  ACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATG                                                                                                                                    <  SRR19973069.3798672/150‑1 (MQ=60)
                  ACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATG                                                                                                                                    <  SRR19973069.2056000/150‑1 (MQ=60)
                    CAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGG                                                                                                                                  >  SRR19973069.725850/1‑150 (MQ=60)
                    CAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGG                                                                                                                                  >  SRR19973069.1411985/1‑150 (MQ=60)
                      ATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGT                                                                                                                                >  SRR19973069.282452/1‑150 (MQ=60)
                      ATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGT                                                                                                                                >  SRR19973069.282428/1‑150 (MQ=60)
                             CTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTAC                                                                                                                         >  SRR19973069.3036420/1‑150 (MQ=60)
                             CTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTAC                                                                                                                         >  SRR19973069.2726562/1‑150 (MQ=60)
                              TTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGTTGGCGACAAAGTTGAATATCAATGGGGTGCGTTACG                                                                                                                        >  SRR19973069.1137914/1‑150 (MQ=60)
                              TTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACG                                                                                                                        <  SRR19973069.3377948/150‑1 (MQ=60)
                              TTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACG                                                                                                                        <  SRR19973069.2356933/150‑1 (MQ=60)
                              TTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACG                                                                                                                        >  SRR19973069.316807/1‑150 (MQ=60)
                                TAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGC                                                                                                                      >  SRR19973069.460599/1‑150 (MQ=60)
                                TAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGC                                                                                                                      >  SRR19973069.460619/1‑150 (MQ=60)
                                  ACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCAT                                                                                                                    <  SRR19973069.2984006/150‑1 (MQ=60)
                                  ACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCAT                                                                                                                    <  SRR19973069.3623699/150‑1 (MQ=60)
                                     CGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGT                                                                                                                 <  SRR19973069.404422/150‑1 (MQ=60)
                                     CGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGT                                                                                                                 >  SRR19973069.7385/1‑150 (MQ=60)
                                         GTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCAT                                                                                                             >  SRR19973069.1782525/1‑150 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         <  SRR19973069.2508742/150‑1 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         <  SRR19973069.349294/150‑1 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         <  SRR19973069.349499/150‑1 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         >  SRR19973069.2204643/1‑150 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         >  SRR19973069.2175178/1‑150 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         >  SRR19973069.2008899/1‑150 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         >  SRR19973069.2009994/1‑150 (MQ=60)
                                             GTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACG                                                                                                         >  SRR19973069.2010084/1‑150 (MQ=60)
                                                CCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGT                                                                                                      >  SRR19973069.866176/1‑150 (MQ=60)
                                                  GTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCG                                                                                                    >  SRR19973069.1212053/1‑150 (MQ=60)
                                                   TTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGT                                                                                                   >  SRR19973069.1653827/1‑150 (MQ=60)
                                                    TGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTT                                                                                                  >  SRR19973069.1836356/1‑150 (MQ=60)
                                                    TGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTT                                                                                                  <  SRR19973069.3834338/150‑1 (MQ=60)
                                                      CTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATagatcggaagagcacacgtctgaactccagtcacatc                                                                                                >  SRR19973069.1597711/1‑113 (MQ=60)
                                                      CTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTAT                                                                                                >  SRR19973069.1845981/1‑150 (MQ=60)
                                                       TTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCANCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATG                                                                                               <  SRR19973069.1945176/150‑1 (MQ=60)
                                                       TTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATG                                                                                               <  SRR19973069.1415852/150‑1 (MQ=60)
                                                       TTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATG                                                                                               <  SRR19973069.3386750/150‑1 (MQ=60)
                                                         AAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCA                                                                                             >  SRR19973069.676727/1‑150 (MQ=60)
                                                         AAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCA                                                                                             >  SRR19973069.2765422/1‑150 (MQ=60)
                                                         AAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATTCA                                                                                             >  SRR19973069.2849754/1‑150 (MQ=60)
                                                         AAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCA                                                                                             <  SRR19973069.3116866/150‑1 (MQ=60)
                                                          AGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCAT                                                                                            <  SRR19973069.1570680/150‑1 (MQ=60)
                                                               CAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCT                                                                                       >  SRR19973069.40773/1‑150 (MQ=60)
                                                               CAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCT                                                                                       <  SRR19973069.1722157/150‑1 (MQ=60)
                                                                   CATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTAC                                                                                   <  SRR19973069.476349/150‑1 (MQ=60)
                                                                    ATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACC                                                                                  >  SRR19973069.1101460/1‑150 (MQ=60)
                                                                       ACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTTGCAGACGTCAGCCGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATTTGGTGCGTTACGGCATTGTGCATGACGAGTCGTTATGCATCCGCTGTACCGCC                                                                               >  SRR19973069.1673501/1‑150 (MQ=60)
                                                                       ACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCC                                                                               >  SRR19973069.81419/1‑150 (MQ=60)
                                                                       ACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCC                                                                               >  SRR19973069.431055/1‑150 (MQ=60)
                                                                       ACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCC                                                                               >  SRR19973069.2365794/1‑150 (MQ=60)
                                                                        CCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCT                                                                              >  SRR19973069.933688/1‑150 (MQ=60)
                                                                              CTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGG                                                                        <  SRR19973069.526940/150‑1 (MQ=60)
                                                                                 GTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTCTGGATG                                                                     >  SRR19973069.1953553/1‑150 (MQ=60)
                                                                                 GTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATG                                                                     >  SRR19973069.1953528/1‑150 (MQ=60)
                                                                                 GTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATG                                                                     >  SRR19973069.1953516/1‑150 (MQ=60)
                                                                                   CGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCT                                                                   <  SRR19973069.3344562/150‑1 (MQ=60)
                                                                                   CGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCT                                                                   <  SRR19973069.3344545/150‑1 (MQ=60)
                                                                                   CGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCT                                                                   <  SRR19973069.477058/150‑1 (MQ=60)
                                                                                       AGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTC                                                               <  SRR19973069.103522/150‑1 (MQ=60)
                                                                                       AGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTC                                                               <  SRR19973069.103716/150‑1 (MQ=60)
                                                                                       AGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTC                                                               >  SRR19973069.2862750/1‑150 (MQ=60)
                                                                                          TTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGG                                                            <  SRR19973069.2485420/150‑1 (MQ=60)
                                                                                            CTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAA                                                          >  SRR19973069.3334100/1‑150 (MQ=60)
                                                                                              CACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGT                                                        <  SRR19973069.1987952/150‑1 (MQ=60)
                                                                                                     GTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAG                                                 <  SRR19973069.831798/150‑1 (MQ=60)
                                                                                                       CGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGT                                               <  SRR19973069.3358470/150‑1 (MQ=60)
                                                                                                       CGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGT                                               <  SRR19973069.3358738/150‑1 (MQ=60)
                                                                                                               TGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAG                                       <  SRR19973069.2522406/150‑1 (MQ=60)
                                                                                                                  CAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCTATGGGGTGCGTTACGGCTTGGTGCATGACGAGTCGTTATTCATCGGCTGTACCTCCTGTATGGATGCTTGTCGGGTAGTGAACAATGTGCCGGAAGGCG                                    <  SRR19973069.680621/150‑1 (MQ=60)
                                                                                                                   AGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGT                                   >  SRR19973069.2600964/1‑150 (MQ=60)
                                                                                                                   AGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGT                                   >  SRR19973069.670616/1‑150 (MQ=60)
                                                                                                                       GTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGCTGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCG                               >  SRR19973069.660100/1‑150 (MQ=60)
                                                                                                                       GTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCG                               <  SRR19973069.598377/150‑1 (MQ=60)
                                                                                                                       GTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCG                               <  SRR19973069.597947/150‑1 (MQ=60)
                                                                                                                             GGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                         <  SRR19973069.76225/150‑1 (MQ=60)
                                                                                                                             GGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                         <  SRR19973069.2020954/150‑1 (MQ=60)
                                                                                                                             GGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                         <  SRR19973069.2774737/150‑1 (MQ=60)
                                                                                                                             GGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                         <  SRR19973069.1530995/150‑1 (MQ=60)
                                                                                                                             GGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                         <  SRR19973069.780139/150‑1 (MQ=60)
                                                                                                                             GGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTG                         <  SRR19973069.780183/150‑1 (MQ=60)
                                                                                                                                ACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACG                      <  SRR19973069.3161409/150‑1 (MQ=60)
                                                                                                                                ACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACG                      >  SRR19973069.2254191/1‑150 (MQ=60)
                                                                                                                                    CAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTA                  >  SRR19973069.2126079/1‑150 (MQ=60)
                                                                                                                                    CAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTA                  >  SRR19973069.622274/1‑150 (MQ=60)
                                                                                                                                     AGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATTCTTGTCGGGAAGTGAACAAGGTGCCGGAATGCGTCTCGCGCCTGACGATTAT                 >  SRR19973069.3666895/1‑150 (MQ=60)
                                                                                                                                     AGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTAT                 >  SRR19973069.265497/1‑150 (MQ=60)
                                                                                                                                     AGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTAT                 >  SRR19973069.3666883/1‑150 (MQ=60)
                                                                                                                                     AGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTAT                 <  SRR19973069.3512212/150‑1 (MQ=60)
                                                                                                                                      GGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATT                <  SRR19973069.3497257/150‑1 (MQ=60)
                                                                                                                                      GGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATT                <  SRR19973069.1086619/150‑1 (MQ=60)
                                                                                                                                       GGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTC               <  SRR19973069.3606831/150‑1 (MQ=60)
                                                                                                                                       GGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTC               <  SRR19973069.3606416/150‑1 (MQ=60)
                                                                                                                                           CGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAG           <  SRR19973069.3043824/150‑1 (MQ=60)
                                                                                                                                            GTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC          <  SRR19973069.1998305/150‑1 (MQ=60)
                                                                                                                                            GTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC          <  SRR19973069.1347059/150‑1 (MQ=60)
                                                                                                                                            GTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGC          <  SRR19973069.1346956/150‑1 (MQ=60)
                                                                                                                                             TGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCG         <  SRR19973069.2895775/150‑1 (MQ=60)
                                                                                                                                             TGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCG         <  SRR19973069.2895780/150‑1 (MQ=60)
                                                                                                                                              GGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGA        <  SRR19973069.2729793/150‑1 (MQ=60)
                                                                                                                                                   CGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGC   >  SRR19973069.3547522/1‑150 (MQ=60)
                                                                                                                                                    TAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCA  >  SRR19973069.2891312/1‑150 (MQ=60)
                                                                                                                                                    TAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCA  >  SRR19973069.3348452/1‑150 (MQ=60)
                                                                                                                                                    TAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAAAAAGGTGCCGGAAGGCGTCTCGCGCCTGACGAATATTCGTAGCGAGCCGCA  >  SRR19973069.3350838/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCA  >  CP009273/4279668‑4279965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑