Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,102,786 C→T 35.9% V94I (GTT→ATT)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,102,7860CT35.9% 316.3 / 284.2 262V94I (GTT→ATT) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base C (88/80);  new base T (52/42);  total (140/122)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 267 total aligned reads displayed.

TTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATA  >  CP009273/2102637‑2102935
                                                                                                                                                     |                                                                                                                                                      
    tcttttcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTAtt                                                                                                                                                    <  2:1138420/150‑1 (MQ=255)
     cttttcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTGATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTAttt                                                                                                                                                   >  2:4419605/1‑150 (MQ=255)
        ttcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTg                                                                                                                                                >  1:3666423/1‑150 (MQ=255)
         tcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGa                                                                                                                                               >  1:1692654/1‑150 (MQ=255)
         tcCATGAAACAGCCTGATAGGGTATATTAAATTCCCGACTAATTCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGa                                                                                                                                               <  2:2071100/150‑1 (MQ=255)
                aaCAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATc                                                                                                                                        >  1:661051/1‑150 (MQ=255)
                aaCAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATc                                                                                                                                        <  2:2045751/150‑1 (MQ=255)
                 aCAGCCTGATAGGGTAGATTAATTTCCCGAATAATGCAGTAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCa                                                                                                                                       <  2:1547731/150‑1 (MQ=255)
                   aGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCAcc                                                                                                                                     >  2:4042176/1‑150 (MQ=255)
                    gCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCg                                                                                                                                    >  1:3318106/1‑150 (MQ=255)
                       aGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGccc                                                                                                                                 >  1:3930707/1‑150 (MQ=255)
                        gATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑a                                                                                                                               >  1:58789/1‑150 (MQ=255)
                        gATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCAGCGCCC‑a                                                                                                                               >  2:1189985/1‑150 (MQ=255)
                         aTAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑aa                                                                                                                              >  1:4122091/1‑150 (MQ=255)
                          tAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑aaa                                                                                                                             >  1:1726306/1‑150 (MQ=255)
                          tAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑aaa                                                                                                                             >  1:4381302/1‑150 (MQ=255)
                           aGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑aaaa                                                                                                                            >  2:4130279/1‑150 (MQ=255)
                            gggTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑aaaaa                                                                                                                           <  2:1049809/150‑1 (MQ=255)
                              gTAGATTAAATTCCCGACTATTGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAAt                                                                                                                         <  2:2172442/150‑1 (MQ=255)
                              gTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAAt                                                                                                                         >  1:816405/1‑150 (MQ=255)
                               tAGATTAAATTCCCGACTATTGCAGTAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATa                                                                                                                        <  1:1585101/150‑1 (MQ=255)
                               tAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATa                                                                                                                        <  1:723059/150‑1 (MQ=255)
                                aGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAg                                                                                                                       >  2:3349453/1‑150 (MQ=255)
                                aGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAg                                                                                                                       >  2:223559/1‑150 (MQ=255)
                                    tAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGt                                                                                                                   <  2:3604023/150‑1 (MQ=255)
                                       aTTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTAc                                                                                                                <  2:1396699/150‑1 (MQ=255)
                                        ttCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACt                                                                                                               >  2:1828718/1‑150 (MQ=255)
                                          cccGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACttt                                                                                                             >  2:1526006/1‑150 (MQ=255)
                                            ctaatAATGAAGTAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTAt                                                                                                           <  1:1327862/146‑1 (MQ=255)
                                            cGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTAt                                                                                                           <  1:2257252/150‑1 (MQ=255)
                                             gACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATc                                                                                                          >  1:3482732/1‑150 (MQ=255)
                                              aCTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCt                                                                                                         >  2:3815359/1‑150 (MQ=255)
                                              aCTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCt                                                                                                         <  2:2992546/150‑1 (MQ=255)
                                               cTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCtt                                                                                                        <  1:3358884/150‑1 (MQ=255)
                                                tAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCttt                                                                                                       >  2:967010/1‑150 (MQ=255)
                                                 aaTGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTa                                                                                                      >  2:1460299/1‑150 (MQ=255)
                                                  aTGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTAc                                                                                                     <  1:502845/150‑1 (MQ=255)
                                                  aTGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTAc                                                                                                     <  2:1481932/150‑1 (MQ=255)
                                                   tGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACt                                                                                                    >  1:523564/1‑150 (MQ=255)
                                                   tGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACt                                                                                                    >  2:1655754/1‑150 (MQ=255)
                                                        gAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctg                                                                                               <  1:1821846/150‑1 (MQ=255)
                                                        gAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctg                                                                                               >  1:4176760/1‑150 (MQ=255)
                                                         aaCAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctgc                                                                                              >  2:3464734/1‑150 (MQ=255)
                                                          aCAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctgct                                                                                             <  1:1977357/150‑1 (MQ=255)
                                                           cAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctgctg                                                                                            <  2:2129041/150‑1 (MQ=255)
                                                            aaaCGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCtgctgc                                                                                           >  1:1905135/1‑150 (MQ=255)
                                                             aaCGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTgctgct                                                                                          >  1:3905385/1‑150 (MQ=255)
                                                             aaCGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTgctgct                                                                                          <  2:3886345/150‑1 (MQ=255)
                                                                  ccATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGc                                                                                     >  1:1055831/1‑150 (MQ=255)
                                                                  ccATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGc                                                                                     <  1:2086839/150‑1 (MQ=255)
                                                                  ccATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGc                                                                                     >  1:1055656/1‑150 (MQ=255)
                                                                   cATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCa                                                                                    <  1:3588807/150‑1 (MQ=255)
                                                                     tataATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAAt                                                                                  <  2:1903249/150‑1 (MQ=255)
                                                                     tataATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAAt                                                                                  <  2:4232004/150‑1 (MQ=255)
                                                                      ataATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATa                                                                                 >  1:963855/1‑150 (MQ=255)
                                                                       taATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCCAAAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATa                                                                                 <  1:4359083/150‑1 (MQ=255)
                                                                        aaTTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATAct                                                                               >  2:2571221/1‑150 (MQ=255)
                                                                           tACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTg                                                                            <  1:3239068/150‑1 (MQ=255)
                                                                               gCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGtt                                                                        <  2:3069087/150‑1 (MQ=255)
                                                                                cATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTg                                                                       <  2:3195819/150‑1 (MQ=255)
                                                                                    cTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCtgt                                                                   >  1:1493947/1‑150 (MQ=255)
                                                                                      tGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTga                                                                 >  1:2695711/1‑150 (MQ=255)
                                                                                       gTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTgag                                                                <  2:4525928/150‑1 (MQ=255)
                                                                                       gTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTgag                                                                >  2:225895/1‑150 (MQ=255)
                                                                                       gTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTgag                                                                >  2:225917/1‑150 (MQ=255)
                                                                                          agatTCAGGAACCAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGAtt                                                             <  2:2379771/148‑1 (MQ=255)
                                                                                           tatTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTg                                                            <  1:783333/150‑1 (MQ=255)
                                                                                              tCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGaaa                                                         >  1:1635571/1‑150 (MQ=255)
                                                                                                  gAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGACACCat                                                     >  2:2751213/1‑150 (MQ=255)
                                                                                                   aaCAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCata                                                    <  2:4261596/150‑1 (MQ=255)
                                                                                                      aagcaagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTc                                                 <  2:1386553/150‑1 (MQ=255)
                                                                                                         caagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTcaac                                              <  1:295480/150‑1 (MQ=255)
                                                                                                         caagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTcaac                                              >  2:841495/1‑150 (MQ=255)
                                                                                                          aagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTcaaca                                             >  2:2098820/1‑150 (MQ=255)
                                                                                                          aagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTcaaca                                             <  1:4508695/150‑1 (MQ=255)
                                                                                                            ggTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCaacaaa                                           <  2:1035515/150‑1 (MQ=255)
                                                                                                              tCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCaacaaaca                                         >  1:3775310/1‑150 (MQ=255)
                                                                                                              tCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCaacaaaca                                         >  2:1788751/1‑150 (MQ=255)
                                                                                                                  cGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTAt                                     <  1:4251627/150‑1 (MQ=255)
                                                                                                                  cGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTAt                                     >  1:3755001/1‑150 (MQ=255)
                                                                                                                   gACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATc                                    >  1:2454491/1‑150 (MQ=255)
                                                                                                                          aGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCAtatata                             <  2:3318106/150‑1 (MQ=255)
                                                                                                                           gAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAg                            >  1:2830919/1‑150 (MQ=255)
                                                                                                                                 cAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGt                      <  1:273387/150‑1 (MQ=255)
                                                                                                                                 cAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGt                      <  1:2533686/150‑1 (MQ=255)
                                                                                                                                   aTGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCg                    <  2:2325977/150‑1 (MQ=255)
                                                                                                                                   aTGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCg                    <  2:3510581/150‑1 (MQ=255)
                                                                                                                                   aTGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCg                    <  2:2577202/150‑1 (MQ=255)
                                                                                                                                      tAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCtt                 >  2:1076963/1‑150 (MQ=255)
                                                                                                                                       aaTGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTg                <  1:2930923/150‑1 (MQ=255)
                                                                                                                                        aTGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTgg               <  2:2415957/150‑1 (MQ=255)
                                                                                                                                         tGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGc              <  2:1864056/150‑1 (MQ=255)
                                                                                                                                         tGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGc              >  1:3475694/1‑150 (MQ=255)
                                                                                                                                          gACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAACCATTATCATATATAGCATAGTCGCTTGGCa             <  2:297452/150‑1 (MQ=255)
                                                                                                                                            cGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaa           >  1:3146218/1‑150 (MQ=255)
                                                                                                                                            cGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaa           >  1:4597645/1‑150 (MQ=255)
                                                                                                                                              aTAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaaaa         >  1:2369985/1‑150 (MQ=255)
                                                                                                                                              aTAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaaaa         >  1:1518226/1‑150 (MQ=255)
                                                                                                                                                   aaCTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCACTACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGaa    >  2:532011/1‑150 (MQ=255)
                                                                                                                                                     cTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAata  <  1:1742727/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAATA  >  CP009273/2102637‑2102935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A