Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,102,786 C→T 44.0% V94I (GTT→ATT)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,102,7860CT44.0% 130.6 / inf 232V94I (GTT→ATT) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base C (71/59);  new base T (64/38);  total (135/97)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 234 total aligned reads displayed.

TTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAA  >  CP009273/2102637‑2102933
                                                                                                                                                     |                                                                                                                                                    
tttttCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAAc                                                                                                                                                      <  1:4125175/150‑1 (MQ=255)
    tcttttcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAattatt                                                                                                                                                  <  1:1690961/150‑1 (MQ=255)
     cttttcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTAttt                                                                                                                                                 >  2:3998396/1‑150 (MQ=255)
      ttttcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTAtttt                                                                                                                                                <  2:2349645/150‑1 (MQ=255)
         tcCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGa                                                                                                                                             >  2:1763742/1‑150 (MQ=255)
            cTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGaaaa                                                                                                                                          >  1:896911/1‑150 (MQ=255)
             tGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAat                                                                                                                                         >  2:31262/1‑150 (MQ=255)
              gAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAata                                                                                                                                        >  2:751009/1‑150 (MQ=255)
              gAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAata                                                                                                                                        <  1:2037115/150‑1 (MQ=255)
              gAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAata                                                                                                                                        <  1:1690535/150‑1 (MQ=255)
               aaaCAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAatat                                                                                                                                       <  2:3768256/150‑1 (MQ=255)
                aaCAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATc                                                                                                                                      >  1:1580623/1‑150 (MQ=255)
                 aCAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCa                                                                                                                                     <  2:3582744/150‑1 (MQ=255)
                 aCAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCa                                                                                                                                     >  1:3656059/1‑150 (MQ=255)
                        gATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑a                                                                                                                             >  1:3656074/1‑150 (MQ=255)
                            gggTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑aaaaa                                                                                                                         >  1:3022365/1‑150 (MQ=255)
                              gTAGATTAAATTCCCGACTAATGCAGTAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAAt                                                                                                                       <  1:2248350/150‑1 (MQ=255)
                              gTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAAt                                                                                                                       >  1:1976131/1‑150 (MQ=255)
                               tataTTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATa                                                                                                                      <  1:3257015/147‑1 (MQ=255)
                               tAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATa                                                                                                                      >  1:534774/1‑150 (MQ=255)
                               tAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATa                                                                                                                      <  2:338293/150‑1 (MQ=255)
                                 gATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGa                                                                                                                    >  1:1879332/1‑150 (MQ=255)
                                  aTTAAATTCCCGACTAATGCAGTAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGAc                                                                                                                   <  1:2493985/150‑1 (MQ=255)
                                    tAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGt                                                                                                                 <  1:1323936/150‑1 (MQ=255)
                                     aaaTTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGtt                                                                                                                >  2:3979724/1‑150 (MQ=255)
                                       aTTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTAc                                                                                                              >  1:1328124/1‑150 (MQ=255)
                                        ttCCCTACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGATTTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACt                                                                                                             <  1:3829344/150‑1 (MQ=255)
                                          cccTACTATTGAAGTAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACttt                                                                                                           <  1:1101529/150‑1 (MQ=255)
                                          cccGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACttt                                                                                                           <  2:1409497/150‑1 (MQ=255)
                                                tAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCttt                                                                                                     >  1:2086166/1‑150 (MQ=255)
                                                  aTGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTAc                                                                                                   >  1:4118558/1‑150 (MQ=255)
                                                  aTGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTAc                                                                                                   <  2:3031895/150‑1 (MQ=255)
                                                      aGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATc                                                                                               >  2:1529828/1‑150 (MQ=255)
                                                      aGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATc                                                                                               >  2:484146/1‑150 (MQ=255)
                                                          aaaaaCGAAAATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctgct                                                                                           <  1:718515/148‑1 (MQ=255)
                                                           cAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctgctg                                                                                          >  1:1002906/1‑150 (MQ=255)
                                                           cAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATctgctg                                                                                          >  2:3474651/1‑150 (MQ=255)
                                                            aaaCGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCtgctgc                                                                                         >  1:2107935/1‑150 (MQ=255)
                                                              aCGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCtt                                                                                       <  1:692202/150‑1 (MQ=255)
                                                              aCGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCtt                                                                                       <  1:554908/150‑1 (MQ=255)
                                                               cGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCttt                                                                                      <  1:1716326/150‑1 (MQ=255)
                                                                   cATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCa                                                                                  >  2:2715806/1‑150 (MQ=255)
                                                                        aaTTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATAct                                                                             >  1:3537300/1‑150 (MQ=255)
                                                                          ttACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATActct                                                                           >  2:3206274/1‑150 (MQ=255)
                                                                           tACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTg                                                                          >  2:3417771/1‑150 (MQ=255)
                                                                               gaaTGATTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGtt                                                                      <  2:1143072/148‑1 (MQ=255)
                                                                               gCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGtt                                                                      <  1:1496906/150‑1 (MQ=255)
                                                                               gCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGtt                                                                      >  2:1675423/1‑150 (MQ=255)
                                                                                cATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTg                                                                     >  1:72921/1‑150 (MQ=255)
                                                                                 aTGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGc                                                                    >  2:1893539/1‑150 (MQ=255)
                                                                                  tGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCt                                                                   <  1:419737/150‑1 (MQ=255)
                                                                                   gCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCtg                                                                  >  2:2082950/1‑150 (MQ=255)
                                                                                      tGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTga                                                               <  1:1477025/150‑1 (MQ=255)
                                                                                        ttATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTgaga                                                             >  2:2395604/1‑150 (MQ=255)
                                                                                        ttATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTgaga                                                             <  1:641911/150‑1 (MQ=255)
                                                                                         tataTTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGAt                                                            <  2:3970586/150‑1 (MQ=255)
                                                                                          atatTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGAtt                                                           <  1:1383061/150‑1 (MQ=255)
                                                                                           tatTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTg                                                          >  1:993854/1‑150 (MQ=255)
                                                                                            atTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGa                                                         >  1:3068395/1‑150 (MQ=255)
                                                                                            atTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGATTTGGCAATACTCTGAGTTGCTGTGAGATTGa                                                         >  2:1172806/1‑150 (MQ=255)
                                                                                               cAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAAc                                                      >  1:3642374/1‑150 (MQ=255)
                                                                                                 ggAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCa                                                    <  1:2810720/150‑1 (MQ=255)
                                                                                                     caagcaagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATAtt                                                <  2:3695179/150‑1 (MQ=255)
                                                                                                      aagcaagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTc                                               >  2:1616183/1‑150 (MQ=255)
                                                                                                      aagcaagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTc                                               >  2:2044230/1‑150 (MQ=255)
                                                                                                      aagcaagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTc                                               >  2:3388629/1‑150 (MQ=255)
                                                                                                       agcaagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTca                                              <  2:3299155/150‑1 (MQ=255)
                                                                                                          aagGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTcaaca                                           <  2:2929164/150‑1 (MQ=255)
                                                                                                           agGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTcaacaa                                          >  2:3868417/1‑150 (MQ=255)
                                                                                                            ggTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCaacaaa                                         >  2:3466173/1‑150 (MQ=255)
                                                                                                             gTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCaacaaac                                        <  1:3722781/150‑1 (MQ=255)
                                                                                                               cATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACAt                                      <  1:3396766/150‑1 (MQ=255)
                                                                                                               cATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACAt                                      >  1:3753610/1‑150 (MQ=255)
                                                                                                               cATCGACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACAt                                      <  1:2678715/150‑1 (MQ=255)
                                                                                                                 tCGACGTTAAGAAAATAAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑‑AAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTAt                                   <  2:1344084/150‑1 (MQ=255)
                                                                                                                  cGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTAt                                   >  2:2471275/1‑150 (MQ=255)
                                                                                                                   gACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATc                                  >  2:2977507/1‑150 (MQ=255)
                                                                                                                   gACGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATc                                  <  2:3574361/150‑1 (MQ=255)
                                                                                                                   gACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATc                                  >  1:3729651/1‑150 (MQ=255)
                                                                                                                    aCGTTAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACATTATCTTTACTATCACCTGCTTTGGCACTACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACAATATCa                                 >  1:256270/1‑150 (MQ=255)
                                                                                                                       ttAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCCAAAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCata                               >  2:4159316/1‑150 (MQ=255)
                                                                                                                       ttAAGAAAATCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCatat                              >  2:3644758/1‑150 (MQ=255)
                                                                                                                        tAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCatata                             <  2:715773/150‑1 (MQ=255)
                                                                                                                        tAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCatata                             <  2:3406091/150‑1 (MQ=255)
                                                                                                                            aaaaTCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGc                         >  2:3457261/1‑150 (MQ=255)
                                                                                                                             aaaTCAATGTAATGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCa                        >  1:3778095/1‑150 (MQ=255)
                                                                                                                             aaaTCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCa                        <  1:520014/150‑1 (MQ=255)
                                                                                                                                      tAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCtt               >  1:2233417/1‑150 (MQ=255)
                                                                                                                                       aaTTACGATAAGAACTATATTTGAAAATATCACCGCCA‑AAAAAATAGACGTTACTTTATATTTAATATCTGATGCTTTGGCAATACTCTGAGTTGCTGTGAGATTTAAACCATATTAAAAAAACATAATCATATATAGAATAGTCGCttt              >  1:1311027/1‑149 (MQ=255)
                                                                                                                                       aaTGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTg              >  1:3404974/1‑150 (MQ=255)
                                                                                                                                         tGACGATAAGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGc            >  2:228288/1‑150 (MQ=255)
                                                                                                                                          gACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCa           >  2:627509/1‑150 (MQ=255)
                                                                                                                                           aCGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaa          >  1:2368559/1‑150 (MQ=255)
                                                                                                                                            cGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaa         >  1:319600/1‑150 (MQ=255)
                                                                                                                                             gATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaaa        >  2:2126757/1‑150 (MQ=255)
                                                                                                                                              aTAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCaaaaa       >  2:3394545/1‑150 (MQ=255)
                                                                                                                                                aaGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGATGTTAGTTTGAAACCATATTCACCAAACATTATCATATATAGCATAGTCGCTTGGCAAAAAcc     <  2:20574/150‑1 (MQ=255)
                                                                                                                                                 aGAATTATTTTTGAAAATATCACCGCCC‑AANAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCg    >  1:3851686/1‑150 (MQ=255)
                                                                                                                                                 aGAATTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCg    >  1:2856527/1‑150 (MQ=255)
                                                                                                                                                   aaCTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGaa  >  1:2134239/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAAAATATCACCGCCC‑AAAAAATAGACGTTACTTTATCTTTACTATCTGCTGCTTTGGCAATACTCTGAGTTGCTGTGAGATTGAAACCATATTCAACAAACATTATCATATATAGCATAGTCGCTTGGCAAAAACCGAA  >  CP009273/2102637‑2102933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A