Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,610,919 C→A 100% intergenic (+3/‑18) bepA → / → yfgD periplasmic metalloprotease and chaperone for OM protein maintenance and assembly/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,610,9190CA99.1% 778.7 / ‑1.6 240intergenic (+3/‑18)bepA/yfgDperiplasmic metalloprotease and chaperone for OM protein maintenance and assembly/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (130/107);  total (133/107)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

GACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGG  >  CP009273/2610774‑2611063
                                                                                                                                                 |                                                                                                                                                
gacgacTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGc                                                                                                                                                >  1:2366906/1‑148 (MQ=255)
 acgacTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCa                                                                                                                                               >  2:1720877/1‑148 (MQ=255)
 acgacTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCa                                                                                                                                               <  1:864582/148‑1 (MQ=255)
 acgacTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCa                                                                                                                                               <  1:2835619/148‑1 (MQ=255)
  cgacTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCAt                                                                                                                                              <  1:3550177/148‑1 (MQ=255)
    acTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTa                                                                                                                                            >  1:2245721/1‑148 (MQ=255)
     cTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTaa                                                                                                                                           >  2:1913033/1‑148 (MQ=255)
      tCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTaaa                                                                                                                                          <  1:2132260/148‑1 (MQ=255)
         aTCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGga                                                                                                                                       <  2:265937/148‑1 (MQ=255)
           cAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaag                                                                                                                                     <  1:729811/148‑1 (MQ=255)
             ggCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaagaa                                                                                                                                   <  1:2311360/148‑1 (MQ=255)
              gCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAc                                                                                                                                  >  1:1619578/1‑148 (MQ=255)
                cATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACaa                                                                                                                                <  1:1099180/148‑1 (MQ=255)
                 aTTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAAt                                                                                                                               >  2:2838288/1‑148 (MQ=255)
                  tttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATg                                                                                                                              <  1:2495893/148‑1 (MQ=255)
                  tttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATg                                                                                                                              <  1:1357789/148‑1 (MQ=255)
                  tttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATg                                                                                                                              <  1:942355/148‑1 (MQ=255)
                  tttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATg                                                                                                                              >  2:2238902/1‑148 (MQ=255)
                  tttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATg                                                                                                                              >  1:454176/1‑148 (MQ=255)
                   ttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGa                                                                                                                             >  2:241969/1‑148 (MQ=255)
                   ttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGa                                                                                                                             <  2:3702614/148‑1 (MQ=255)
                       cTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCaa                                                                                                                         <  1:381467/148‑1 (MQ=255)
                       cTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCaa                                                                                                                         <  1:2751522/148‑1 (MQ=255)
                       cTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCaa                                                                                                                         <  1:1012540/148‑1 (MQ=255)
                         gTTGAGTAGCGCCAGGTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAc                                                                                                                       <  1:1296545/148‑1 (MQ=255)
                          ttGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACa                                                                                                                      <  2:438041/148‑1 (MQ=255)
                           tGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAg                                                                                                                     <  2:1542084/148‑1 (MQ=255)
                           tGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAg                                                                                                                     <  2:3441030/148‑1 (MQ=255)
                            gAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAgg                                                                                                                    <  2:796247/148‑1 (MQ=255)
                             aGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGt                                                                                                                   <  1:486909/148‑1 (MQ=255)
                                  cgcCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTaaaaa                                                                                                              <  1:2105440/148‑1 (MQ=255)
                                       gTTCGCAGGTGAAATTAGGCAGCCTGCCACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAAGTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAATTCTAc                                                                                                         >  2:493935/1‑148 (MQ=255)
                                         tCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCa                                                                                                       >  2:1754236/1‑148 (MQ=255)
                                         tCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACAAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCa                                                                                                       <  2:128779/148‑1 (MQ=255)
                                           gCAGGTGAATTTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATa                                                                                                     <  2:2035737/148‑1 (MQ=255)
                                           gCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATa                                                                                                     <  1:610963/148‑1 (MQ=255)
                                               gTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAccc                                                                                                 >  2:2775819/1‑148 (MQ=255)
                                                tGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCa                                                                                                <  2:3621030/148‑1 (MQ=255)
                                                     ttAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTg                                                                                           >  1:330451/1‑148 (MQ=255)
                                                      tAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAGCGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGt                                                                                          >  2:648437/1‑148 (MQ=255)
                                                      tAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGt                                                                                          >  1:2229093/1‑148 (MQ=255)
                                                        ggCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTc                                                                                        >  1:1565191/1‑148 (MQ=255)
                                                         gCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCa                                                                                       >  1:3219868/1‑148 (MQ=255)
                                                            gCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAag                                                                                    <  1:1972422/148‑1 (MQ=255)
                                                             ccTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAaga                                                                                   >  2:1387718/1‑148 (MQ=255)
                                                             ccTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAaga                                                                                   >  1:3539975/1‑148 (MQ=255)
                                                             ccTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAaga                                                                                   >  2:546692/1‑148 (MQ=255)
                                                                gCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGcc                                                                                <  1:1881012/148‑1 (MQ=255)
                                                                 caacaaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCg                                                                               <  1:232461/148‑1 (MQ=255)
                                                                   acaaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCggg                                                                             <  1:739326/148‑1 (MQ=255)
                                                                   acaaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCggg                                                                             <  1:3099357/148‑1 (MQ=255)
                                                                    caaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGa                                                                            >  1:350542/1‑148 (MQ=255)
                                                                    caaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGa                                                                            >  2:3769900/1‑148 (MQ=255)
                                                                    caaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGa                                                                            >  1:2518568/1‑148 (MQ=255)
                                                                    caaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGa                                                                            >  1:1283484/1‑148 (MQ=255)
                                                                     aaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTACAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGaa                                                                           >  1:770274/1‑148 (MQ=255)
                                                                     aaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGaa                                                                           >  1:168563/1‑148 (MQ=255)
                                                                      aGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGaaa                                                                          <  1:2000229/148‑1 (MQ=255)
                                                                         gcgTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGc                                                                       <  1:3177471/148‑1 (MQ=255)
                                                                          cgTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCt                                                                      >  2:3033377/1‑148 (MQ=255)
                                                                            ttACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGa                                                                    >  2:803778/1‑148 (MQ=255)
                                                                             tACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGaa                                                                   >  1:1864055/1‑148 (MQ=255)
                                                                              aCGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAAc                                                                  <  2:274305/148‑1 (MQ=255)
                                                                                 aTGCGCGCATCTACCAGTTTCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACctg                                                               <  2:2136228/148‑1 (MQ=255)
                                                                                 aTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACctg                                                               <  1:3551370/148‑1 (MQ=255)
                                                                                 aTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACctg                                                               >  2:1798360/1‑148 (MQ=255)
                                                                                 aTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACctg                                                               >  2:356370/1‑148 (MQ=255)
                                                                                 aTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACctg                                                               >  2:355935/1‑148 (MQ=255)
                                                                                           cGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTgaaagaaa                                                     <  2:1645506/148‑1 (MQ=255)
                                                                                             aCCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAc                                                   >  1:1908142/1‑148 (MQ=255)
                                                                                             aCCAGTTGCGCCAGCTGCAGGAACGCTTTAAGACTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAc                                                   >  2:708625/1‑148 (MQ=255)
                                                                                                   tGCGCCAGCTGCAGGAACGATTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTg                                             <  2:772718/148‑1 (MQ=255)
                                                                                                       ccAGGTGCAGTAACGATTTAAGCCTTATACCAAGATGTATTTAGAATTAAAGGAAGAACAATCACCAAACAGTTAAAAATCTACCAAAACCCACGCTTTTAAAAGAGCCGGTAAACGCTTAACGTGCTTAAAGAAAACGGCGTGGAGc                                         <  1:1780937/148‑1 (MQ=255)
                                                                                                          gCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAg                                      >  2:168829/1‑148 (MQ=255)
                                                                                                          gCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAg                                      >  2:2480157/1‑148 (MQ=255)
                                                                                                          gCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAg                                      >  2:2481174/1‑148 (MQ=255)
                                                                                                           cTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGa                                     >  2:2069231/1‑148 (MQ=255)
                                                                                                           cTGAAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGa                                     <  2:572011/148‑1 (MQ=255)
                                                                                                             gCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAAAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAg                                   <  2:1841436/148‑1 (MQ=255)
                                                                                                              cAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgt                                  >  2:1896302/1‑148 (MQ=255)
                                                                                                                ggAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgtgg                                >  1:889491/1‑148 (MQ=255)
                                                                                                                 gAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgtggt                               >  2:1589300/1‑148 (MQ=255)
                                                                                                                 gAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgtggt                               >  1:340201/1‑148 (MQ=255)
                                                                                                                 gAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgtggt                               >  1:3647069/1‑148 (MQ=255)
                                                                                                                   aCGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGc                             >  2:2614824/1‑148 (MQ=255)
                                                                                                                    cGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGct                            >  1:1615715/1‑148 (MQ=255)
                                                                                                                      cTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGctct                          >  2:1289181/1‑148 (MQ=255)
                                                                                                                       tttAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTa                         >  1:2780556/1‑148 (MQ=255)
                                                                                                                        ttAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTAc                        >  2:2373123/1‑148 (MQ=255)
                                                                                                                         tAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTAcc                       <  2:2241270/148‑1 (MQ=255)
                                                                                                                          aaGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCt                      >  2:1005204/1‑148 (MQ=255)
                                                                                                                             ccTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTga                   >  2:105542/1‑148 (MQ=255)
                                                                                                                             ccTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTga                   >  1:896502/1‑148 (MQ=255)
                                                                                                                                 ataCCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGaca               <  2:2531771/148‑1 (MQ=255)
                                                                                                                                   aCCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACAcc             >  1:971076/1‑148 (MQ=255)
                                                                                                                                    ccAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACAccc            >  1:3216673/1‑148 (MQ=255)
                                                                                                                                      aaGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAGGAAAACGGCGTGGAGCCCGAGGTGGTGGTCTGCGGGGAGACGCccgc          >  1:2078127/1‑148 (MQ=255)
                                                                                                                                      aaGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACccgc          >  1:64083/1‑148 (MQ=255)
                                                                                                                                         aTGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGa       >  2:3244441/1‑148 (MQ=255)
                                                                                                                                              aTTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCgg  >  1:547566/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGG  >  CP009273/2610774‑2611063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACG  >  CP009273/2610771‑2611068
                                                                                                                                                    |                                                                                                                                                     
CCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAG                                                                                                                                                      <  SRR19973069.873647/150‑1 (MQ=60)
CCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAG                                                                                                                                                      <  SRR19973069.873627/150‑1 (MQ=60)
 CGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGC                                                                                                                                                     >  SRR19973069.2382976/1‑150 (MQ=60)
    ACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATT                                                                                                                                                  <  SRR19973069.870621/150‑1 (MQ=60)
    ACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATT                                                                                                                                                  <  SRR19973069.2854337/150‑1 (MQ=60)
     CGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTA                                                                                                                                                 >  SRR19973069.2260841/1‑150 (MQ=60)
     CGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTA                                                                                                                                                 >  SRR19973069.664523/1‑150 (MQ=60)
     CGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTA                                                                                                                                                 <  SRR19973069.3573245/150‑1 (MQ=60)
     CGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTA                                                                                                                                                 <  SRR19973069.1440849/150‑1 (MQ=60)
      GACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAA                                                                                                                                                >  SRR19973069.3048894/1‑150 (MQ=60)
       ACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAA                                                                                                                                               >  SRR19973069.930916/1‑150 (MQ=60)
         TCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGG                                                                                                                                             <  SRR19973069.2146924/150‑1 (MQ=60)
         TCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGG                                                                                                                                             <  SRR19973069.2512885/150‑1 (MQ=60)
           GATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAA                                                                                                                                           >  SRR19973069.585652/1‑150 (MQ=60)
              CAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAA                                                                                                                                        <  SRR19973069.1801516/150‑1 (MQ=60)
              CAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAA                                                                                                                                        <  SRR19973069.3566372/150‑1 (MQ=60)
              CAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAA                                                                                                                                        <  SRR19973069.734969/150‑1 (MQ=60)
              CAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAA                                                                                                                                        <  SRR19973069.735667/150‑1 (MQ=60)
               AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCTTTACGATGCGCGAATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       <  SRR19973069.275665/150‑1 (MQ=60)
               AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       <  SRR19973069.999345/150‑1 (MQ=60)
               AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       >  SRR19973069.2011275/1‑150 (MQ=60)
               AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       <  SRR19973069.1080875/150‑1 (MQ=60)
               AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       >  SRR19973069.1630542/1‑150 (MQ=60)
               AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGAAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       <  SRR19973069.999400/150‑1 (MQ=60)
                GGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACA                                                                                                                                      <  SRR19973069.2327087/150‑1 (MQ=60)
                GGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACA                                                                                                                                      <  SRR19973069.1892425/150‑1 (MQ=60)
                GGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACA                                                                                                                                      <  SRR19973069.1871478/150‑1 (MQ=60)
                 GCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAA                                                                                                                                     >  SRR19973069.1624427/1‑150 (MQ=60)
                   CATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATG                                                                                                                                   <  SRR19973069.1106861/150‑1 (MQ=60)
                   CATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATG                                                                                                                                   >  SRR19973069.457501/1‑150 (MQ=60)
                     TTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGAC                                                                                                                                 <  SRR19973069.948718/150‑1 (MQ=60)
                     TTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGAC                                                                                                                                 <  SRR19973069.948717/150‑1 (MQ=60)
                     TTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGAC                                                                                                                                 <  SRR19973069.1367218/150‑1 (MQ=60)
                     TTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGAC                                                                                                                                 <  SRR19973069.2512579/150‑1 (MQ=60)
                      TTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACC                                                                                                                                <  SRR19973069.624459/150‑1 (MQ=60)
                       TCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCA                                                                                                                               <  SRR19973069.3159887/150‑1 (MQ=60)
                          CTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAC                                                                                                                            <  SRR19973069.2769893/150‑1 (MQ=60)
                          CTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAC                                                                                                                            <  SRR19973069.384250/150‑1 (MQ=60)
                          CTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAC                                                                                                                            <  SRR19973069.1019522/150‑1 (MQ=60)
                            GTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAG                                                                                                                          <  SRR19973069.3815807/150‑1 (MQ=60)
                            GTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAG                                                                                                                          <  SRR19973069.2338850/150‑1 (MQ=60)
                            GTTGAGTAGCGCCAGGTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAG                                                                                                                          <  SRR19973069.1305278/150‑1 (MQ=60)
                               GAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTA                                                                                                                       <  SRR19973069.1121040/150‑1 (MQ=60)
                                AGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAA                                                                                                                      <  SRR19973069.490423/150‑1 (MQ=60)
                                  TAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAA                                                                                                                    >  SRR19973069.672255/1‑150 (MQ=60)
                                  TAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAA                                                                                                                    >  SRR19973069.3379850/1‑150 (MQ=60)
                                  TAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAA                                                                                                                    >  SRR19973069.1467052/1‑150 (MQ=60)
                                    GCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAAT                                                                                                                  <  SRR19973069.2453536/150‑1 (MQ=60)
                                     cggctgttctctGGTGAAATTAGGCAGACTGCAACAAGCGCGTTACGTTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGTTGTTATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATC                                                                                                                 <  SRR19973069.2121029/138‑1 (MQ=60)
                                     CGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATC                                                                                                                 <  SRR19973069.2119968/150‑1 (MQ=60)
                                      GCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCAggcccggacgaacacggcccaaacaggtaataatct                                                                                                                >  SRR19973069.3663621/1‑114 (MQ=60)
                                      GCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCT                                                                                                                <  SRR19973069.2314409/150‑1 (MQ=60)
                                      GCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCT                                                                                                                <  SRR19973069.3549834/150‑1 (MQ=60)
                                              GCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAC                                                                                                        <  SRR19973069.2170452/150‑1 (MQ=60)
                                              GCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAC                                                                                                        <  SRR19973069.615033/150‑1 (MQ=60)
                                              GCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAC                                                                                                        >  SRR19973069.2788170/1‑150 (MQ=60)
                                              GCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAC                                                                                                        <  SRR19973069.615049/150‑1 (MQ=60)
                                               CAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACC                                                                                                       <  SRR19973069.2322433/150‑1 (MQ=60)
                                                 GGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCA                                                                                                     >  SRR19973069.3025520/1‑150 (MQ=60)
                                                      AATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTG                                                                                                >  SRR19973069.332707/1‑150 (MQ=60)
                                                      AATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTG                                                                                                >  SRR19973069.333672/1‑150 (MQ=60)
                                                       ATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGT                                                                                               >  SRR19973069.2244154/1‑150 (MQ=60)
                                                       ATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGT                                                                                               >  SRR19973069.2244823/1‑150 (MQ=60)
                                                         TAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTC                                                                                             >  SRR19973069.1575865/1‑150 (MQ=60)
                                                          AGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCA                                                                                            >  SRR19973069.3240898/1‑150 (MQ=60)
                                                            GCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAA                                                                                          >  SRR19973069.1337170/1‑150 (MQ=60)
                                                            GCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAA                                                                                          >  SRR19973069.880378/1‑150 (MQ=60)
                                                              AGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGA                                                                                        >  SRR19973069.3562750/1‑150 (MQ=60)
                                                              AGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGA                                                                                        >  SRR19973069.3562940/1‑150 (MQ=60)
                                                               GCCTGCAACAAGCGCGTTAGGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAG                                                                                       <  SRR19973069.1984428/150‑1 (MQ=60)
                                                               GCCTGCAACAAGCGCGTTACGTTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAG                                                                                       <  SRR19973069.1986118/150‑1 (MQ=60)
                                                                   GCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGG                                                                                   <  SRR19973069.1894082/150‑1 (MQ=60)
                                                                    CAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGG                                                                                  <  SRR19973069.2630458/150‑1 (MQ=60)
                                                                    CAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGG                                                                                  <  SRR19973069.2176211/150‑1 (MQ=60)
                                                                    CAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGG                                                                                  <  SRR19973069.234230/150‑1 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.353040/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.455521/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.1292992/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.1292071/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.849558/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.2535319/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.1684304/1‑150 (MQ=60)
                                                                     AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973069.1293548/1‑150 (MQ=60)
                                                                      ACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTACAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAA                                                                                >  SRR19973069.775722/1‑150 (MQ=60)
                                                                      ACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAA                                                                                >  SRR19973069.170007/1‑150 (MQ=60)
                                                                      ACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAA                                                                                <  SRR19973069.3119776/150‑1 (MQ=60)
                                                                      ACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAA                                                                                <  SRR19973069.744567/150‑1 (MQ=60)
                                                                         AGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACG                                                                             <  SRR19973069.2014162/150‑1 (MQ=60)
                                                                            GCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTG                                                                          <  SRR19973069.3198142/150‑1 (MQ=60)
                                                                            GCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTG                                                                          <  SRR19973069.3428463/150‑1 (MQ=60)
                                                                              GTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAA                                                                        >  SRR19973069.1877055/1‑150 (MQ=60)
                                                                                  CGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTG                                                                    <  SRR19973069.3211425/150‑1 (MQ=60)
                                                                                   GATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGC                                                                   >  SRR19973069.329837/1‑150 (MQ=60)
                                                                                    ATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCT                                                                  <  SRR19973069.3574451/150‑1 (MQ=60)
                                                                                    ATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCT                                                                  <  SRR19973069.3574418/150‑1 (MQ=60)
                                                                                              CGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC                                                        >  SRR19973069.1920243/1‑150 (MQ=60)
                                                                                              CGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC                                                        >  SRR19973069.1921319/1‑150 (MQ=60)
                                                                                               GACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACG                                                       >  SRR19973069.3665294/1‑150 (MQ=60)
                                                                                               GACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACG                                                       >  SRR19973069.1930597/1‑150 (MQ=60)
                                                                                               GACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACG                                                       >  SRR19973069.2347285/1‑150 (MQ=60)
                                                                                                 CCAGTTGCGCCAGCTGCAGGAACGATTTAAGCCTTATACCAAGATTTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGC                                                     <  SRR19973069.1732935/150‑1 (MQ=60)
                                                                                                          CCAGGTGCAGTAACGATTTAAGCCTTATACCAAGATGTATTTAGAATTAAAGGAAGAACAATCACCAAACAGTTAAAAATCTACCAAAACCCACGCTTTTAAAAGAGCCGGTAAACGCTTAACGTGCTTAAAGAAAACGGCGTGGAGCCA                                            <  SRR19973069.1793480/150‑1 (MQ=60)
                                                                                                                 CAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGG                                     >  SRR19973069.896547/1‑150 (MQ=60)
                                                                                                                 CAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGG                                     >  SRR19973069.895635/1‑150 (MQ=60)
                                                                                                                  AGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGT                                    >  SRR19973069.3670953/1‑150 (MQ=60)
                                                                                                                  AGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGT                                    >  SRR19973069.2833296/1‑150 (MQ=60)
                                                                                                                  AGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGT                                    >  SRR19973069.342589/1‑150 (MQ=60)
                                                                                                                     AACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCT                                 >  SRR19973069.1626621/1‑150 (MQ=60)
                                                                                                                     AACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCT                                 >  SRR19973069.1627688/1‑150 (MQ=60)
                                                                                                                      ACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTC                                >  SRR19973069.2427400/1‑150 (MQ=60)
                                                                                                                        GCTTTAAGCCTTATCCCAAGATGTAATTAGCATTAAAGCAACAACAATCACAAAACATTTAAAAATCTACCATAACCCCCGCTGTTAAAAGAGCCGGGAAACGCTGAACCTGCTGAAATAAAACGGCGTGGAGCCAGAAGTGGTGCTCTA                              <  SRR19973069.744602/150‑1 (MQ=60)
                                                                                                                        GCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTA                              >  SRR19973069.2799069/1‑150 (MQ=60)
                                                                                                                        GCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTA                              >  SRR19973069.1671322/1‑150 (MQ=60)
                                                                                                                        GCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTA                              >  SRR19973069.2119947/1‑150 (MQ=60)
                                                                                                                              AGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGA                        >  SRR19973069.902678/1‑150 (MQ=60)
                                                                                                                              AGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGA                        >  SRR19973069.902661/1‑150 (MQ=60)
                                                                                                                                 CTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGAC                     >  SRR19973069.467263/1‑150 (MQ=60)
                                                                                                                                 CTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGAC                     >  SRR19973069.442848/1‑150 (MQ=60)
                                                                                                                                 CTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGAC                     >  SRR19973069.3199660/1‑150 (MQ=60)
                                                                                                                                    ATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACC                  >  SRR19973069.977564/1‑150 (MQ=60)
                                                                                                                                     TACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCC                 >  SRR19973069.3237674/1‑150 (MQ=60)
                                                                                                                                     TACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCC                 >  SRR19973069.3237670/1‑150 (MQ=60)
                                                                                                                                       CCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAGGAAAACGGCGTGGAGCCcgaggtggtggtctgcggggagacgcccgc               >  SRR19973069.2092509/1‑120 (MQ=60)
                                                                                                                                       CCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGC               >  SRR19973069.2092331/1‑150 (MQ=60)
                                                                                                                                       CCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGC               >  SRR19973069.2092130/1‑150 (MQ=60)
                                                                                                                                       CCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGC               >  SRR19973069.64835/1‑150 (MQ=60)
                                                                                                                                              GTAATTAGCATTAAAGGAAGAACAATCACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCG        <  SRR19973069.505233/150‑1 (MQ=60)
                                                                                                                                               TAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGG       >  SRR19973069.80162/1‑150 (MQ=60)
                                                                                                                                               TAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGG       >  SRR19973069.551405/1‑150 (MQ=60)
                                                                                                                                                AATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGC      >  SRR19973069.1766439/1‑150 (MQ=60)
                                                                                                                                                AATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGC      >  SRR19973069.790320/1‑150 (MQ=60)
                                                                                                                                                AATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGC      >  SRR19973069.1766516/1‑150 (MQ=60)
                                                                                                                                                 ATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCA     >  SRR19973069.1219281/1‑150 (MQ=60)
                                                                                                                                                    AGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACG  >  SRR19973069.3726695/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACG  >  CP009273/2610771‑2611068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑