Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,610,919 C→A 100% intergenic (+3/‑18) bepA → / → yfgD periplasmic metalloprotease and chaperone for OM protein maintenance and assembly/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,610,9190CA99.8% 861.9 / ‑6.4 241intergenic (+3/‑18)bepA/yfgDperiplasmic metalloprotease and chaperone for OM protein maintenance and assembly/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (107/133);  minor base T (0/1);  total (107/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

CGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCA  >  CP009273/2610772‑2611065
                                                                                                                                                   |                                                                                                                                                  
cGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGc                                                                                                                                                  >  2:2154812/1‑150 (MQ=255)
 ggACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCa                                                                                                                                                 <  2:2625606/150‑1 (MQ=255)
          gATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaa                                                                                                                                        >  1:4068388/1‑150 (MQ=255)
          gATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaa                                                                                                                                        >  1:1161787/1‑150 (MQ=255)
          gATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaa                                                                                                                                        <  2:3676683/150‑1 (MQ=255)
           aTCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaag                                                                                                                                       >  1:811269/1‑150 (MQ=255)
           aTCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaag                                                                                                                                       <  1:2044654/150‑1 (MQ=255)
            tCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaaga                                                                                                                                      <  1:3565028/150‑1 (MQ=255)
             cAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGgaagaa                                                                                                                                     <  2:1462730/150‑1 (MQ=255)
              aGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAc                                                                                                                                    <  1:1426381/150‑1 (MQ=255)
              aGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAc                                                                                                                                    >  2:3934474/1‑150 (MQ=255)
               ggCCATTTCGCTGTTGAGTAGCGCCTGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACa                                                                                                                                   <  1:3192690/150‑1 (MQ=255)
                gCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGTCCAGTTGCGCCAGCTGCAGGATCGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACaa                                                                                                                                  <  2:2186468/150‑1 (MQ=255)
                gCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACaa                                                                                                                                  >  2:1221964/1‑150 (MQ=255)
                gCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACaa                                                                                                                                  <  2:2218849/150‑1 (MQ=255)
                  cATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATg                                                                                                                                >  1:1686106/1‑150 (MQ=255)
                     ttCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGAcc                                                                                                                             >  1:2001963/1‑150 (MQ=255)
                      tCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCa                                                                                                                            <  1:3767386/150‑1 (MQ=255)
                        gCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCaaa                                                                                                                          >  2:1774422/1‑150 (MQ=255)
                         cTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAc                                                                                                                         <  1:593578/150‑1 (MQ=255)
                          tGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACa                                                                                                                        <  2:2681857/150‑1 (MQ=255)
                           gTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAg                                                                                                                       <  2:3470749/150‑1 (MQ=255)
                                gTAGCGCCAGTTCGCAGGTGAACTTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTaaa                                                                                                                  <  2:742175/150‑1 (MQ=255)
                                gTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTaaa                                                                                                                  <  1:1571742/150‑1 (MQ=255)
                                gTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTaaa                                                                                                                  <  2:1325624/150‑1 (MQ=255)
                                 tAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTaaaa                                                                                                                 >  2:3084518/1‑150 (MQ=255)
                                  aGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTaaaaa                                                                                                                >  1:300419/1‑150 (MQ=255)
                                     gcCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCt                                                                                                             <  2:364056/150‑1 (MQ=255)
                                     gcCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCt                                                                                                             >  2:89904/1‑150 (MQ=255)
                                     gcCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCt                                                                                                             <  2:1487788/150‑1 (MQ=255)
                                       cAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGAGGAAATTAGCATTAAAGGAAGAACAATGACAAAACAGGTAAAAATCTAc                                                                                                           >  2:3024630/1‑150 (MQ=255)
                                        aGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTAcc                                                                                                          >  2:939012/1‑150 (MQ=255)
                                        aGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTAcc                                                                                                          <  1:2514883/150‑1 (MQ=255)
                                        aGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAAATAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTAca                                                                                                          >  1:887637/1‑149 (MQ=255)
                                         gTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCa                                                                                                         <  1:3392785/150‑1 (MQ=255)
                                            cGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATaa                                                                                                      >  1:2711049/1‑150 (MQ=255)
                                             gCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAc                                                                                                     <  1:1943751/150‑1 (MQ=255)
                                                ggTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCa                                                                                                  <  1:1434553/150‑1 (MQ=255)
                                                 gTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCAc                                                                                                 <  2:1014435/150‑1 (MQ=255)
                                                 gTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCAc                                                                                                 >  1:2318656/1‑150 (MQ=255)
                                                  tGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACg                                                                                                <  2:3719875/150‑1 (MQ=255)
                                                   gAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGc                                                                                               >  1:113683/1‑150 (MQ=255)
                                                   gAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGc                                                                                               <  2:3295599/150‑1 (MQ=255)
                                                      aTTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGt                                                                                            <  2:4020380/150‑1 (MQ=255)
                                                       ttAGGCAGCCTGTAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGtt                                                                                           <  1:4148380/150‑1 (MQ=255)
                                                       ttAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGtt                                                                                           <  2:2484618/150‑1 (MQ=255)
                                                             aGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAaga                                                                                     >  2:3418413/1‑150 (MQ=255)
                                                                cTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGcc                                                                                  >  2:3604017/1‑150 (MQ=255)
                                                                 tGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCg                                                                                 <  1:3027759/150‑1 (MQ=255)
                                                                 tGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCg                                                                                 >  1:3157158/1‑150 (MQ=255)
                                                                  gCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCgg                                                                                <  2:1334126/150‑1 (MQ=255)
                                                                    aacaaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCCGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGa                                                                              >  2:4064498/1‑150 (MQ=255)
                                                                    aacaaGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGa                                                                              >  1:951611/1‑150 (MQ=255)
                                                                        aGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACg                                                                          >  2:2151337/1‑150 (MQ=255)
                                                                               tACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAAcc                                                                   <  1:2058383/150‑1 (MQ=255)
                                                                                   aTGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACctgct                                                               <  1:551547/150‑1 (MQ=255)
                                                                                      cgcgcATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTgaa                                                            <  1:1677404/150‑1 (MQ=255)
                                                                                        cgcATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTgaaag                                                          >  1:348537/1‑150 (MQ=255)
                                                                                           aTCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTgaaagaaa                                                       <  2:1024299/150‑1 (MQ=255)
                                                                                           aTCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTgaaagaaa                                                       >  2:1285995/1‑150 (MQ=255)
                                                                                            tCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCACACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGaaaa                                                      <  1:889809/150‑1 (MQ=255)
                                                                                             cGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAc                                                     <  2:248646/150‑1 (MQ=255)
                                                                                              gACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACg                                                    <  2:3628197/150‑1 (MQ=255)
                                                                                              gACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACg                                                    <  1:375087/150‑1 (MQ=255)
                                                                                               aCCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACgg                                                   >  2:3002777/1‑150 (MQ=255)
                                                                                                 cAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCg                                                 >  1:1001356/1‑150 (MQ=255)
                                                                                                    ttGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAATATGTAATTTGAATTAAAGTAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTgg                                              <  2:3485582/150‑1 (MQ=255)
                                                                                                    ttGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACCGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTgg                                              <  2:3846048/150‑1 (MQ=255)
                                                                                                    ttGCGCCAGCTGCAGGAACGATTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTgg                                              <  2:3485630/150‑1 (MQ=255)
                                                                                                     tGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGa                                             >  2:2907467/1‑150 (MQ=255)
                                                                                                     tGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGa                                             <  2:2826941/150‑1 (MQ=255)
                                                                                                       cgcCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGANc                                           <  2:3157451/150‑1 (MQ=255)
                                                                                                        gcCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGcc                                          >  1:69932/1‑150 (MQ=255)
                                                                                                        gcCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGcc                                          <  1:3019246/150‑1 (MQ=255)
                                                                                                        gcCAGCTGCAGGAACGCTTTAAGCCATATACCAAGATGTAATTAGAATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGcc                                          <  2:3500681/150‑1 (MQ=255)
                                                                                                          cAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAg                                        >  1:2237531/1‑150 (MQ=255)
                                                                                                          cAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAg                                        >  2:1183654/1‑150 (MQ=255)
                                                                                                           aGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGa                                       >  1:1259023/1‑150 (MQ=255)
                                                                                                              tGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgt                                    >  1:1736431/1‑150 (MQ=255)
                                                                                                              tGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgt                                    >  2:1678351/1‑150 (MQ=255)
                                                                                                               gCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAgtg                                   >  2:1932956/1‑150 (MQ=255)
                                                                                                                   gAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGc                               >  2:517761/1‑150 (MQ=255)
                                                                                                                   gAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGc                               >  2:1803614/1‑150 (MQ=255)
                                                                                                                    aaCGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGct                              >  1:2515780/1‑150 (MQ=255)
                                                                                                                      cGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGctct                            <  1:2993288/150‑1 (MQ=255)
                                                                                                                      cGCTTTAAGCATTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGctct                            <  1:2993335/150‑1 (MQ=255)
                                                                                                                        cTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTAc                          >  1:3538206/1‑150 (MQ=255)
                                                                                                                           tAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCtt                       <  1:209997/150‑1 (MQ=255)
                                                                                                                           tAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCtt                       <  1:3924245/150‑1 (MQ=255)
                                                                                                                            aaGCCTTATACCAAGCTGTAATTAGCATTAAAGGAAGAACAATGCCCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTg                      <  1:892026/150‑1 (MQ=255)
                                                                                                                            aaGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTg                      >  1:1119585/1‑150 (MQ=255)
                                                                                                                             aGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTga                     <  2:2335245/150‑1 (MQ=255)
                                                                                                                                cTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGac                  >  2:1048422/1‑150 (MQ=255)
                                                                                                                                  tataCCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGacac                >  1:383329/1‑150 (MQ=255)
                                                                                                                                        aaGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACccgccg          >  1:3660583/1‑150 (MQ=255)
                                                                                                                                         aGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGa         >  2:3256723/1‑150 (MQ=255)
                                                                                                                                          gATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGAt        >  2:2862490/1‑150 (MQ=255)
                                                                                                                                             gTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCg     >  1:376791/1‑150 (MQ=255)
                                                                                                                                               aaTTAGCATTAAAGGAAGAACAATGCCCAACCAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGc   <  2:1845095/150‑1 (MQ=255)
                                                                                                                                                aTTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCa  >  1:2679208/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCA  >  CP009273/2610772‑2611065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACG  >  CP009273/2610770‑2611068
                                                                                                                                                     |                                                                                                                                                     
GCCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTA                                                                                                                                                       <  SRR19973074.3554522/150‑1 (MQ=60)
 CCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAG                                                                                                                                                      <  SRR19973074.203086/150‑1 (MQ=60)
  CGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGC                                                                                                                                                     >  SRR19973074.2096377/1‑150 (MQ=60)
  CGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGC                                                                                                                                                     >  SRR19973074.2096412/1‑150 (MQ=60)
   GGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCA                                                                                                                                                    <  SRR19973074.172550/150‑1 (MQ=60)
     ACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACACTTTAAGCCTTATACCAAGATGTAATTAGCATT                                                                                                                                                  <  SRR19973074.2923875/150‑1 (MQ=60)
      CGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTA                                                                                                                                                 >  SRR19973074.3479667/1‑150 (MQ=60)
      CGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTA                                                                                                                                                 <  SRR19973074.570072/150‑1 (MQ=60)
         CTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAG                                                                                                                                              <  SRR19973074.3767314/150‑1 (MQ=60)
            GATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAA                                                                                                                                           >  SRR19973074.350362/1‑150 (MQ=60)
            GATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAA                                                                                                                                           >  SRR19973074.1163999/1‑150 (MQ=60)
            GATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAA                                                                                                                                           >  SRR19973074.4075916/1‑150 (MQ=60)
             ATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAG                                                                                                                                          >  SRR19973074.812831/1‑150 (MQ=60)
             ATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAG                                                                                                                                          <  SRR19973074.2048505/150‑1 (MQ=60)
              TCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGA                                                                                                                                         <  SRR19973074.3571290/150‑1 (MQ=60)
              TCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGA                                                                                                                                         <  SRR19973074.3815457/150‑1 (MQ=60)
              TCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGA                                                                                                                                         <  SRR19973074.3571603/150‑1 (MQ=60)
                AGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAAC                                                                                                                                       <  SRR19973074.1429020/150‑1 (MQ=60)
                 GGCCATTTCGCTGTTGAGTAGCGCCTGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACA                                                                                                                                      <  SRR19973074.3198571/150‑1 (MQ=60)
                   CCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAAT                                                                                                                                    <  SRR19973074.2051350/150‑1 (MQ=60)
                   CCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAAT                                                                                                                                    <  SRR19973074.3552398/150‑1 (MQ=60)
                    CATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATG                                                                                                                                   >  SRR19973074.1689256/1‑150 (MQ=60)
                    CATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATG                                                                                                                                   >  SRR19973074.1689259/1‑150 (MQ=60)
                       TTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACC                                                                                                                                >  SRR19973074.2005800/1‑150 (MQ=60)
                       TTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACC                                                                                                                                >  SRR19973074.2005758/1‑150 (MQ=60)
                        TCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCA                                                                                                                               <  SRR19973074.3774305/150‑1 (MQ=60)
                        TCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCA                                                                                                                               <  SRR19973074.3774360/150‑1 (MQ=60)
                           CTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAC                                                                                                                            <  SRR19973074.594717/150‑1 (MQ=60)
                           CTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAC                                                                                                                            <  SRR19973074.1123785/150‑1 (MQ=60)
                           CTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAAC                                                                                                                            <  SRR19973074.3197135/150‑1 (MQ=60)
                              TTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGG                                                                                                                         <  SRR19973074.408798/150‑1 (MQ=60)
                                  GTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAA                                                                                                                     <  SRR19973074.1574725/150‑1 (MQ=60)
                                    AGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAA                                                                                                                   >  SRR19973074.300981/1‑150 (MQ=60)
                                    AGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAA                                                                                                                   >  SRR19973074.300984/1‑150 (MQ=60)
                                    AGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAA                                                                                                                   >  SRR19973074.300934/1‑150 (MQ=60)
                                       GCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCT                                                                                                                <  SRR19973074.1436352/150‑1 (MQ=60)
                                       GCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCT                                                                                                                <  SRR19973074.582629/150‑1 (MQ=60)
                                         CAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGGTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTAC                                                                                                              <  SRR19973074.49267/150‑1 (MQ=60)
                                          AGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACC                                                                                                             >  SRR19973074.889374/1‑150 (MQ=60)
                                          AGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACC                                                                                                             <  SRR19973074.1285959/150‑1 (MQ=60)
                                          AGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACC                                                                                                             <  SRR19973074.2519574/150‑1 (MQ=60)
                                          AGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACC                                                                                                             <  SRR19973074.2519588/150‑1 (MQ=60)
                                          AGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAAATAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACA                                                                                                             >  SRR19973074.889338/1‑150 (MQ=60)
                                           GTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCA                                                                                                            <  SRR19973074.3399067/150‑1 (MQ=60)
                                           GTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCA                                                                                                            <  SRR19973074.409769/150‑1 (MQ=60)
                                              CGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAA                                                                                                         >  SRR19973074.2716070/1‑150 (MQ=60)
                                               GCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAAC                                                                                                        <  SRR19973074.1947464/150‑1 (MQ=60)
                                                CAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACC                                                                                                       >  SRR19973074.2033172/1‑150 (MQ=60)
                                                  GGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCA                                                                                                     <  SRR19973074.1437222/150‑1 (MQ=60)
                                                   GTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCAC                                                                                                    >  SRR19973074.2322989/1‑150 (MQ=60)
                                                    TGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACG                                                                                                   <  SRR19973074.2667902/150‑1 (MQ=60)
                                                     GAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGC                                                                                                  >  SRR19973074.113955/1‑150 (MQ=60)
                                                         TTAGGCAGCCTGTAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTT                                                                                              <  SRR19973074.4156021/150‑1 (MQ=60)
                                                           AGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTTAAAATCTACCATAACCCACGCTGTTCA                                                                                            >  SRR19973074.818982/1‑150 (MQ=60)
                                                               AGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGA                                                                                        >  SRR19973074.3720381/1‑150 (MQ=60)
                                                                   TGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCG                                                                                    <  SRR19973074.3033358/150‑1 (MQ=60)
                                                                   TGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCG                                                                                    >  SRR19973074.3162962/1‑150 (MQ=60)
                                                                   TGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCG                                                                                    <  SRR19973074.1056492/150‑1 (MQ=60)
                                                                   TGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCG                                                                                    <  SRR19973074.1282845/150‑1 (MQ=60)
                                                                   TGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAAGGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCG                                                                                    <  SRR19973074.3033230/150‑1 (MQ=60)
                                                                      AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973074.2236421/1‑150 (MQ=60)
                                                                      AACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGA                                                                                 >  SRR19973074.953418/1‑150 (MQ=60)
                                                                            CGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCT                                                                           <  SRR19973074.1153672/150‑1 (MQ=60)
                                                                              CGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGA                                                                         <  SRR19973074.2248425/150‑1 (MQ=60)
                                                                                 TACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACC                                                                      <  SRR19973074.2062250/150‑1 (MQ=60)
                                                                                     ATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCT                                                                  <  SRR19973074.552634/150‑1 (MQ=60)
                                                                                       GCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGA                                                                <  SRR19973074.3014728/150‑1 (MQ=60)
                                                                                        CGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAA                                                               <  SRR19973074.1680551/150‑1 (MQ=60)
                                                                                          CGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAG                                                             >  SRR19973074.349168/1‑150 (MQ=60)
                                                                                              TCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCACACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAA                                                         <  SRR19973074.891511/150‑1 (MQ=60)
                                                                                              TCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAA                                                         <  SRR19973074.891599/150‑1 (MQ=60)
                                                                                              TCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAA                                                         <  SRR19973074.891517/150‑1 (MQ=60)
                                                                                              TCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAA                                                         <  SRR19973074.2962364/150‑1 (MQ=60)
                                                                                                GACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACG                                                       <  SRR19973074.375789/150‑1 (MQ=60)
                                                                                                GACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACG                                                       >  SRR19973074.969901/1‑150 (MQ=60)
                                                                                                GACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACG                                                       >  SRR19973074.969923/1‑150 (MQ=60)
                                                                                                   CAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCG                                                    >  SRR19973074.1003216/1‑150 (MQ=60)
                                                                                                   CAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCG                                                    >  SRR19973074.3280791/1‑150 (MQ=60)
                                                                                                   CAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCG                                                    <  SRR19973074.1676270/150‑1 (MQ=60)
                                                                                                      TTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGG                                                 <  SRR19973074.3652498/150‑1 (MQ=60)
                                                                                                        GCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATACCCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAG                                               <  SRR19973074.270338/150‑1 (MQ=60)
                                                                                                          GCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCC                                             <  SRR19973074.3024834/150‑1 (MQ=60)
                                                                                                          GCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCC                                             >  SRR19973074.70107/1‑150 (MQ=60)
                                                                                                           CCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCA                                            >  SRR19973074.3065212/1‑150 (MQ=60)
                                                                                                           CCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCA                                            >  SRR19973074.3065185/1‑150 (MQ=60)
                                                                                                           CCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCA                                            <  SRR19973074.1551322/150‑1 (MQ=60)
                                                                                                            CAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAG                                           >  SRR19973074.2241737/1‑150 (MQ=60)
                                                                                                             AGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGA                                          >  SRR19973074.1261442/1‑150 (MQ=60)
                                                                                                              GCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAA                                         <  SRR19973074.1898729/150‑1 (MQ=60)
                                                                                                               CTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAG                                        >  SRR19973074.3481844/1‑150 (MQ=60)
                                                                                                                TGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGT                                       >  SRR19973074.1739642/1‑150 (MQ=60)
                                                                                                                TGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGT                                       <  SRR19973074.790371/150‑1 (MQ=60)
                                                                                                                TGCAGGAACGATTTAAGCCTTATACCACGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGT                                       <  SRR19973074.790355/150‑1 (MQ=60)
                                                                                                                   AGGAACGATTTAAGCCTTATACCAAGATGTAATTAGAATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGT                                    <  SRR19973074.3854442/150‑1 (MQ=60)
                                                                                                                      AACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCT                                 >  SRR19973074.1726801/1‑150 (MQ=60)
                                                                                                                      AACGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCT                                 >  SRR19973074.2520472/1‑150 (MQ=60)
                                                                                                                        CGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCT                               >  SRR19973074.3827118/1‑150 (MQ=60)
                                                                                                                        CGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCT                               <  SRR19973074.2564457/150‑1 (MQ=60)
                                                                                                                        CGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCT                               <  SRR19973074.2998869/150‑1 (MQ=60)
                                                                                                                        CGCTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCT                               <  SRR19973074.2998850/150‑1 (MQ=60)
                                                                                                                        CGCTTTAAGCATTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCT                               <  SRR19973074.2998897/150‑1 (MQ=60)
                                                                                                                          CTTTAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTAC                             >  SRR19973074.3544728/1‑150 (MQ=60)
                                                                                                                          CTTTAAGCCTTATACCAAGATGTAATAAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCTGGAAACGCTGAACCTGCTGAAAGAACACGGCGTGGAGCCAGAAGTGGTGCTCTAC                             >  SRR19973074.4041372/1‑150 (MQ=60)
                                                                                                                             TAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTT                          <  SRR19973074.210436/150‑1 (MQ=60)
                                                                                                                             TAAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTT                          <  SRR19973074.3931460/150‑1 (MQ=60)
                                                                                                                              AAGCCTTATACCAAGCTGTAATTAGCATTAAAGGAAGAACAATGCCCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTG                         <  SRR19973074.893731/150‑1 (MQ=60)
                                                                                                                              AAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTG                         >  SRR19973074.1121697/1‑150 (MQ=60)
                                                                                                                              AAGCCTTATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTG                         <  SRR19973074.1236597/150‑1 (MQ=60)
                                                                                                                                 CCTTATACCAATATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGA                      >  SRR19973074.1146203/1‑150 (MQ=60)
                                                                                                                                    TATACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACAC                   >  SRR19973074.384052/1‑150 (MQ=60)
                                                                                                                                       ACCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCG                <  SRR19973074.2452012/150‑1 (MQ=60)
                                                                                                                                        CCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGC               >  SRR19973074.921414/1‑150 (MQ=60)
                                                                                                                                        CCAAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGC               >  SRR19973074.1648053/1‑150 (MQ=60)
                                                                                                                                          AAGATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCG             >  SRR19973074.3667356/1‑150 (MQ=60)
                                                                                                                                            GATGTAATTAGCATTAAAGGAAGAACAATCACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGAT           <  SRR19973074.2357258/150‑1 (MQ=60)
                                                                                                                                             ATGTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATG          <  SRR19973074.652266/150‑1 (MQ=60)
                                                                                                                                               GTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCG        >  SRR19973074.377387/1‑150 (MQ=60)
                                                                                                                                               GTAATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCG        >  SRR19973074.377500/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCA     >  SRR19973074.2684192/1‑150 (MQ=60)
                                                                                                                                                  ATTAGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCA     >  SRR19973074.1980338/1‑150 (MQ=60)
                                                                                                                                                    TAGCATTAAAGGAAGAACAATGACCAAACTTGTAAAAATCTACCATACCCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAAC   <  SRR19973074.745631/150‑1 (MQ=60)
                                                                                                                                                     AGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACG  >  SRR19973074.560177/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCCGGACGACTCGATCAGGCCATTTCGCTGTTGAGTAGCGCCAGTTCGCAGGTGAAATTAGGCAGCCTGCAACAAGCGCGTTACGATGCGCGCATCGACCAGTTGCGCCAGCTGCAGGAACGCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAACGGCGTGGAGCCAGAAGTGGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACG  >  CP009273/2610770‑2611068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑