Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 634,078 T→A 26.2% intergenic (‑49/‑323) dsbG ← / → ahpC thiol:disulfide interchange protein, periplasmic/alkyl hydroperoxide reductase, C22 subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273634,0780TA26.2% 645.0 / 263.4 355intergenic (‑49/‑323)dsbG/ahpCthiol:disulfide interchange protein, periplasmic/alkyl hydroperoxide reductase, C22 subunit
Reads supporting (aligned to +/- strand):  ref base T (149/112);  new base A (48/45);  total (198/157)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

ATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTACACGGT  >  CP009273/633929‑634227
                                                                                                                                                     |                                                                                                                                                     
atTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAATGCATAACCt                                                                                                                                                       >  1:627285/1‑150 (MQ=255)
   gTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATc                                                                                                                                                    >  1:1574448/1‑150 (MQ=255)
   gTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATc                                                                                                                                                    >  2:362783/1‑150 (MQ=255)
     aaTGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCAc                                                                                                                                                  <  2:313667/150‑1 (MQ=255)
     aaTGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCAc                                                                                                                                                  <  2:3301520/150‑1 (MQ=255)
      aTGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACt                                                                                                                                                 <  1:2906653/150‑1 (MQ=255)
        gCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGt                                                                                                                                               <  1:478429/150‑1 (MQ=255)
           cTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCAt                                                                                                                                            <  1:3174880/150‑1 (MQ=255)
               ttttCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATATCCTATCACTGTCATAGGt                                                                                                                                        >  2:4264311/1‑150 (MQ=255)
                tttCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTa                                                                                                                                       >  2:4960906/1‑150 (MQ=255)
                 ttCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTaa                                                                                                                                      >  1:5211948/1‑150 (MQ=255)
                 ttCAATCGCTTTTACTGGAGCAGGAAGGTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTaa                                                                                                                                      <  2:4485643/150‑1 (MQ=255)
                  tCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAag                                                                                                                                     <  1:3665739/150‑1 (MQ=255)
                    aaTCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAagag                                                                                                                                   >  2:1239773/1‑150 (MQ=255)
                    aaTCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAagag                                                                                                                                   <  2:4138308/150‑1 (MQ=255)
                     aTCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGc                                                                                                                                  >  1:2126277/1‑150 (MQ=255)
                     aTCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGc                                                                                                                                  >  1:3416221/1‑150 (MQ=255)
                       cGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCtt                                                                                                                                >  2:1500178/1‑150 (MQ=255)
                        gCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTa                                                                                                                               <  1:4546660/150‑1 (MQ=255)
                         cTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAg                                                                                                                              >  2:4000666/1‑150 (MQ=255)
                         cTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAg                                                                                                                              >  2:3807702/1‑150 (MQ=255)
                           tttACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGAt                                                                                                                            <  2:60733/150‑1 (MQ=255)
                           tttACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGAt                                                                                                                            <  1:3344412/150‑1 (MQ=255)
                            ttACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATc                                                                                                                           <  1:4094497/150‑1 (MQ=255)
                            ttACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATc                                                                                                                           >  1:4349221/1‑150 (MQ=255)
                               cTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAgg                                                                                                                        >  1:2818449/1‑150 (MQ=255)
                                tGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGt                                                                                                                       >  1:105401/1‑150 (MQ=255)
                                  gAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGtta                                                                                                                     >  1:2350480/1‑148 (MQ=255)
                                  gAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGa                                                                                                                     <  1:2289882/150‑1 (MQ=255)
                                   aGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGAt                                                                                                                    <  2:4657947/150‑1 (MQ=255)
                                   aGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGAt                                                                                                                    <  1:1842750/150‑1 (MQ=255)
                                     cAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTTATTg                                                                                                                  >  2:2763981/1‑150 (MQ=255)
                                     cAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTg                                                                                                                  >  2:2447857/1‑150 (MQ=255)
                                         aaGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCt                                                                                                              <  2:5220465/150‑1 (MQ=255)
                                         aaGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCt                                                                                                              <  1:325887/150‑1 (MQ=255)
                                          aGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCtt                                                                                                             <  1:4838424/150‑1 (MQ=255)
                                            ttCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTg                                                                                                           >  1:1506150/1‑150 (MQ=255)
                                               ctctGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGttt                                                                                                        <  1:3948537/150‑1 (MQ=255)
                                                      aaGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAggg                                                                                                 >  2:3948111/1‑150 (MQ=255)
                                                          cGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTTATTGCCCTTTGTTTATGAGGGtgtt                                                                                             <  1:3030263/150‑1 (MQ=255)
                                                               gCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAAt                                                                                        >  2:1381899/1‑150 (MQ=255)
                                                                cAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATc                                                                                       <  1:1103627/150‑1 (MQ=255)
                                                                 aGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATcc                                                                                      >  2:2080065/1‑150 (MQ=255)
                                                                 aGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATcc                                                                                      <  2:1708496/150‑1 (MQ=255)
                                                                     aGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGt                                                                                  >  1:1400598/1‑150 (MQ=255)
                                                                      gCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTc                                                                                 <  1:1896384/150‑1 (MQ=255)
                                                                        agagCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCgt                                                                               <  2:5273011/150‑1 (MQ=255)
                                                                         gagCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCgtt                                                                              >  2:3763222/1‑150 (MQ=255)
                                                                          agCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCgttg                                                                             <  2:2396025/150‑1 (MQ=255)
                                                                            ccAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCgttgtt                                                                           >  1:854379/1‑150 (MQ=255)
                                                                                tAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTTATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCAt                                                                       >  2:4338262/1‑150 (MQ=255)
                                                                                 aaaaGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCAtt                                                                      >  2:1010107/1‑150 (MQ=255)
                                                                                   aaGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTg                                                                    >  2:1134092/1‑150 (MQ=255)
                                                                                    aGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGt                                                                   >  2:3234642/1‑150 (MQ=255)
                                                                                    aGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGAAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATAAGGAGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGt                                                                   >  2:735325/1‑150 (MQ=255)
                                                                                     gTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTa                                                                  >  1:1793680/1‑150 (MQ=255)
                                                                                       aTCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAg                                                                >  1:4770776/1‑150 (MQ=255)
                                                                                       aTCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAg                                                                >  2:4918644/1‑150 (MQ=255)
                                                                                        tCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAgg                                                               <  1:3329703/150‑1 (MQ=255)
                                                                                             tttAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAac                                                          <  2:2218965/150‑1 (MQ=255)
                                                                                             tttAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAac                                                          >  1:3777850/1‑150 (MQ=255)
                                                                                               tAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAacac                                                        <  1:1197395/150‑1 (MQ=255)
                                                                                                aaCATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTTATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACAcc                                                       >  1:166462/1‑150 (MQ=255)
                                                                                                aaCATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCCATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACAcc                                                       >  1:20723/1‑150 (MQ=255)
                                                                                                  cATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTc                                                     >  2:1422034/1‑150 (MQ=255)
                                                                                                   aTTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCa                                                    >  2:5189807/1‑150 (MQ=255)
                                                                                                       aTTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCt                                                <  2:3044273/150‑1 (MQ=255)
                                                                                                        tttGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTg                                               >  1:1176331/1‑150 (MQ=255)
                                                                                                           gTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTgcag                                            <  1:779457/150‑1 (MQ=255)
                                                                                                           gTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTgcag                                            >  1:2854986/1‑150 (MQ=255)
                                                                                                             ccTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTgcaggc                                          >  2:2579198/1‑150 (MQ=255)
                                                                                                              cTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTgcaggca                                         <  1:1607835/150‑1 (MQ=255)
                                                                                                              cTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCACAGCCTgcaggcc                                         >  1:4905345/1‑149 (MQ=255)
                                                                                                               ttttCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTTATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTgcaggcag                                        >  1:3596371/1‑150 (MQ=255)
                                                                                                                   cagtcagtGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCAc                                    >  1:4049467/1‑150 (MQ=255)
                                                                                                                   cagtcagtGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCAc                                    >  1:2621146/1‑150 (MQ=255)
                                                                                                                    agtcagtGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACt                                   >  2:1909573/1‑150 (MQ=255)
                                                                                                                    agtcagtGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCAGAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACt                                   <  2:2815176/150‑1 (MQ=255)
                                                                                                                    agtcagtGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACt                                   <  2:2466916/150‑1 (MQ=255)
                                                                                                                      tcagtGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGa                                 >  2:2174620/1‑150 (MQ=255)
                                                                                                                        agtGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAg                               >  2:1194720/1‑150 (MQ=255)
                                                                                                                        agtGCAAAAGTCGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAg                               >  1:3900180/1‑150 (MQ=255)
                                                                                                                             aaaaGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTTATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATAcc                          <  2:1623170/150‑1 (MQ=255)
                                                                                                                             aaaaGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCGTTGTTTATGAGGGTGTAGTAATCCATGTCTTTGTTGAATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTTAAGATAcc                          >  1:2185244/1‑150 (MQ=255)
                                                                                                                              aaaGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCa                         <  2:820593/150‑1 (MQ=255)
                                                                                                                              aaaGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCa                         <  1:5115457/150‑1 (MQ=255)
                                                                                                                                   cGAGTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAggg                    <  1:362783/150‑1 (MQ=255)
                                                                                                                                    gAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGt                   >  1:2523212/1‑150 (MQ=255)
                                                                                                                                     aGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTa                  <  2:5122741/150‑1 (MQ=255)
                                                                                                                                      gTAAAAGGCATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTATTCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAg                 >  2:4599050/1‑150 (MQ=255)
                                                                                                                                          aaGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCa             <  2:974897/150‑1 (MQ=255)
                                                                                                                                          aaGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCa             >  2:3179300/1‑150 (MQ=255)
                                                                                                                                           aGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAAAACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAg            >  2:1996387/1‑150 (MQ=255)
                                                                                                                                              cATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGAtt         >  1:5279942/1‑150 (MQ=255)
                                                                                                                                              cATAACCAATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGAtt         <  2:1051608/150‑1 (MQ=255)
                                                                                                                                               aTAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTa        <  2:3426690/150‑1 (MQ=255)
                                                                                                                                                tAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTac       >  1:2991367/1‑150 (MQ=255)
                                                                                                                                                 aaCCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGGTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTaca      <  1:4264311/150‑1 (MQ=255)
                                                                                                                                                   ccTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTACACg    >  1:3975518/1‑150 (MQ=255)
                                                                                                                                                     tATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTTTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTAAACGGt  >  1:5038531/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGTAATGCCCTGTTTTTCAATCGCTTTTACTGGAGCAGGAAGTTCCTCTGCGAAGGCGATTGCAGGAAGCAGAGCCAGTAAAAGTATCTTTTTTAACATTAATTTGTCCTTTTCAGTCAGTGCAAAAGTCGAGTAAAAGGCATAACCTATCACTGTCATAGGTAAGAGCTTAGATCAGGTGATTGCCCTTTGTTTATGAGGGTGTTGTAATCCATGTCGTTGTTGCATTTGTAAGGGCAACACCTCAGCCTGCAGGCAGGCACTGAAGATACCAAAGGGTAGTTCAGATTACACGGT  >  CP009273/633929‑634227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A