Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,720,180 C→A 11.1% intergenic (‑3/‑100) ydhL ← / → nemR DUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,720,1800CA11.1% 983.7 / 61.8 297intergenic (‑3/‑100)ydhL/nemRDUF1289 family protein/transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base C (106/158);  new base A (12/21);  total (118/179)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 299 total aligned reads displayed.

CTCTTGTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGG  >  CP009273/1720031‑1720329
                                                                                                                                                     |                                                                                                                                                     
 tctTGTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCg                                                                                                                                                      >  2:307310/1‑150 (MQ=255)
 tctTGTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCg                                                                                                                                                      <  1:2310362/150‑1 (MQ=255)
  ctTGTTGTTCGCCATCGCTCATTTTATTCCAGTGAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGc                                                                                                                                                     <  1:4972628/150‑1 (MQ=255)
    tGTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGccc                                                                                                                                                   >  2:3108721/1‑150 (MQ=255)
     gTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCcct                                                                                                                                                  <  2:25686/150‑1 (MQ=255)
     gTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCcct                                                                                                                                                  >  2:5006581/1‑150 (MQ=255)
     gTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCcct                                                                                                                                                  >  2:2620307/1‑150 (MQ=255)
        tttCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCcctcct                                                                                                                                               <  1:1613071/150‑1 (MQ=255)
        tttCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCACTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCcctcct                                                                                                                                               <  2:2238070/150‑1 (MQ=255)
            gCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGa                                                                                                                                           >  2:4740954/1‑150 (MQ=255)
             ccATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGAt                                                                                                                                          >  2:3368379/1‑150 (MQ=255)
                tCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAg                                                                                                                                       <  1:1879228/150‑1 (MQ=255)
                  gCTCATTTTATTCCAGTTAAAACGCTCATCACGACTATGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGat                                                                                                                                     <  1:2457648/150‑1 (MQ=255)
                  gCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGat                                                                                                                                     <  2:2714955/150‑1 (MQ=255)
                  gCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGat                                                                                                                                     >  2:1554686/1‑150 (MQ=255)
                  gCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGat                                                                                                                                     <  1:4579301/150‑1 (MQ=255)
                  gCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACACGCGTTCGTCAGACTGGCAAATTCCCCGGAACGGGCTCTGGACGGGAAATAACTCTAATTGCTCCGCAACTTCGCCCTCCTCAGATAAGat                                                                                                                                     >  2:2365726/1‑150 (MQ=255)
                    tCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGatta                                                                                                                                   <  2:1405091/150‑1 (MQ=255)
                      aTTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGattatt                                                                                                                                 >  2:1193037/1‑150 (MQ=255)
                      aTTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGattatt                                                                                                                                 <  2:4330823/150‑1 (MQ=255)
                      aTTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGattatt                                                                                                                                 <  2:4230930/150‑1 (MQ=255)
                       ttttATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGAttatta                                                                                                                                >  1:1028295/1‑150 (MQ=255)
                         ttATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGACCTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTAcc                                                                                                                              >  2:4289209/1‑150 (MQ=255)
                         ttATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTAcc                                                                                                                              <  2:1240890/150‑1 (MQ=255)
                           aTTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCat                                                                                                                            >  1:3620813/1‑150 (MQ=255)
                            ttCCAGTTAAAACGCTCATCACGACTACGAACACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAAGTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCatt                                                                                                                           <  2:4796023/150‑1 (MQ=255)
                               cAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCattatt                                                                                                                        <  1:3461268/150‑1 (MQ=255)
                                aGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTg                                                                                                                       >  1:4214877/1‑150 (MQ=255)
                                   tAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAg                                                                                                                    >  2:601402/1‑150 (MQ=255)
                                   tAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAg                                                                                                                    >  2:1919553/1‑150 (MQ=255)
                                    aaaaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGc                                                                                                                   >  2:408250/1‑150 (MQ=255)
                                    aaaaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGc                                                                                                                   >  2:329898/1‑150 (MQ=255)
                                    aaaaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGc                                                                                                                   <  2:2839416/150‑1 (MQ=255)
                                     aaaCGCTCATCTCGTCTACGAAAACAGCCTCGACTAAACAAGCGTTCGTCAGACTGGCATATTCCCCGGCACGGGCTCTGGACGGGTAAGAACTCTATTTGCTCCGCCACTTCGCCCTCCTCAGTTAAGATTATTACCATTATTGAAGCt                                                                                                                  <  2:2792982/150‑1 (MQ=255)
                                     aaaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCt                                                                                                                  <  2:2417597/150‑1 (MQ=255)
                                     aaaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCt                                                                                                                  >  1:5071273/1‑150 (MQ=255)
                                      aaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTg                                                                                                                 >  1:1248749/1‑150 (MQ=255)
                                      aaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTg                                                                                                                 <  1:224821/150‑1 (MQ=255)
                                      aaCGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTg                                                                                                                 >  2:4114108/1‑150 (MQ=255)
                                           tcatcaCGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAAt                                                                                                            <  2:2451247/150‑1 (MQ=255)
                                           tcatcaCGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAAt                                                                                                            <  1:2946286/150‑1 (MQ=255)
                                              tcaCGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTc                                                                                                         <  2:2912789/150‑1 (MQ=255)
                                               caCGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTcc                                                                                                        <  2:4655989/150‑1 (MQ=255)
                                                 cGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCaa                                                                                                      <  1:4465411/150‑1 (MQ=255)
                                                 cGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCaa                                                                                                      >  2:2607650/1‑150 (MQ=255)
                                                   aCTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAg                                                                                                    <  1:5181913/150‑1 (MQ=255)
                                                    cTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGt                                                                                                   >  2:3970228/1‑150 (MQ=255)
                                                        gAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCa                                                                                               <  2:3093164/150‑1 (MQ=255)
                                                         aaaaCAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCaa                                                                                              <  2:5081213/150‑1 (MQ=255)
                                                            aCAGCCGCGACAACACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACtt                                                                                           <  2:4177296/150‑1 (MQ=255)
                                                             cAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACttt                                                                                          <  1:3468773/150‑1 (MQ=255)
                                                             cAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAAGTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACttt                                                                                          <  1:2579567/150‑1 (MQ=255)
                                                              aGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTttg                                                                                         >  1:537802/1‑150 (MQ=255)
                                                              aGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTttg                                                                                         <  1:4360270/150‑1 (MQ=255)
                                                              aGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTttg                                                                                         >  1:1978707/1‑150 (MQ=255)
                                                                 cgcgACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTttgctt                                                                                      <  1:1007298/150‑1 (MQ=255)
                                                                 cgcgACAAAACCCGCGTTCGTCAGACTGGCAAAGTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTttgctt                                                                                      <  2:66352/150‑1 (MQ=255)
                                                                  gcgACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTttgcttg                                                                                     <  2:1480313/150‑1 (MQ=255)
                                                                    gACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCa                                                                                   >  2:348313/1‑150 (MQ=255)
                                                                      cAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACt                                                                                 <  2:3877384/150‑1 (MQ=255)
                                                                      cAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACt                                                                                 >  1:2903195/1‑150 (MQ=255)
                                                                      cAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACt                                                                                 >  1:2795211/1‑150 (MQ=255)
                                                                      cAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACt                                                                                 <  1:2592948/150‑1 (MQ=255)
                                                                       aaaaCCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTa                                                                                >  1:3181465/1‑150 (MQ=255)
                                                                        aaaCCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAg                                                                               <  2:831191/150‑1 (MQ=255)
                                                                           cccGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTTATGTCCAAAGTAGCAACTTTGCTTGCACTAGAcc                                                                            <  2:109661/150‑1 (MQ=255)
                                                                           cccGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGAcc                                                                            <  2:4298357/150‑1 (MQ=255)
                                                                           cccGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGAcc                                                                            <  1:2033114/150‑1 (MQ=255)
                                                                                gTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGACAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGAGTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTg                                                                       >  1:683178/1‑150 (MQ=255)
                                                                                    gTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTct                                                                   >  1:4789731/1‑150 (MQ=255)
                                                                                      cAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTctac                                                                 >  1:340294/1‑150 (MQ=255)
                                                                                              cAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTcc                                                         <  2:3128536/150‑1 (MQ=255)
                                                                                               aaaTTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCa                                                        >  2:932450/1‑150 (MQ=255)
                                                                                                 aTTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAAc                                                      >  1:827749/1‑150 (MQ=255)
                                                                                                   tCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGc                                                    >  1:564039/1‑150 (MQ=255)
                                                                                                     cccGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCAt                                                  <  2:2989521/150‑1 (MQ=255)
                                                                                                     cccGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCAt                                                  <  2:1532011/150‑1 (MQ=255)
                                                                                                      ccGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATg                                                 <  2:709955/150‑1 (MQ=255)
                                                                                                      ccGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATg                                                 >  1:4050778/1‑150 (MQ=255)
                                                                                                             gggCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGaacaaac                                          <  2:4032030/150‑1 (MQ=255)
                                                                                                               tatctGGACGGGAAAGAACTCTAATTGCTCATACACTTAGCACTACTCAGATAAGATTATTACCTTTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAacac                                        <  1:3738987/148‑1 (MQ=255)
                                                                                                                ctctGGACGGGAAAGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAacaca                                       >  2:2966829/1‑150 (MQ=255)
                                                                                                                        gggAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACAt                               <  1:3224792/150‑1 (MQ=255)
                                                                                                                        gggAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACAt                               <  1:3128029/150‑1 (MQ=255)
                                                                                                                        gggAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTATACTACACTCCAACGCATGAACAAACACACCGAACAt                               <  2:5121562/150‑1 (MQ=255)
                                                                                                                         ggAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATg                              <  1:117745/150‑1 (MQ=255)
                                                                                                                           aaaGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGAt                            <  1:4740943/150‑1 (MQ=255)
                                                                                                                            aaGAACTCTAATTGCTCCGCCACTTAGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATa                           >  1:4857870/1‑150 (MQ=255)
                                                                                                                             aGAACTATATTTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTTGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATAc                          <  1:2387243/150‑1 (MQ=255)
                                                                                                                                 ctctAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTcgc                      >  1:624570/1‑150 (MQ=255)
                                                                                                                                   ctAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGa                    >  2:5295955/1‑150 (MQ=255)
                                                                                                                                   ctAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCAATAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGa                    <  2:2106603/150‑1 (MQ=255)
                                                                                                                                     aaTTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAAc                  >  2:4601010/1‑150 (MQ=255)
                                                                                                                                       ttGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACAt                <  1:4289209/150‑1 (MQ=255)
                                                                                                                                        tGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACAtc               >  1:3785096/1‑150 (MQ=255)
                                                                                                                                         gCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACAtct              <  2:365596/150‑1 (MQ=255)
                                                                                                                                          cTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACAtctc             >  1:2188334/1‑150 (MQ=255)
                                                                                                                                            ccGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCt           <  1:881127/150‑1 (MQ=255)
                                                                                                                                                 aCTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAAAATGATACTCGCGAACATCTCCTGGCGa      >  2:2409951/1‑150 (MQ=255)
                                                                                                                                                     cGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACggg  >  1:4339999/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTTGTTTTTCGCCATCGCTCATTTTATTCCAGTTAAAACGCTCATCACGACTACGAAAACAGCCGCGACAAAACCCGCGTTCGTCAGACTGGCAAATTCCCCGGCACGGGCTCTGGACGGGAAAGAACTCTAATTGCTCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGG  >  CP009273/1720031‑1720329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A